CN102043029B - Repressing system of high performance liquid chromatography - Google Patents
Repressing system of high performance liquid chromatography Download PDFInfo
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- CN102043029B CN102043029B CN 200910205151 CN200910205151A CN102043029B CN 102043029 B CN102043029 B CN 102043029B CN 200910205151 CN200910205151 CN 200910205151 CN 200910205151 A CN200910205151 A CN 200910205151A CN 102043029 B CN102043029 B CN 102043029B
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- compression travel
- performance liquid
- stopper rod
- pump
- liquid chromatograph
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Abstract
The invention relates to a repressing system of a high performance liquid chromatography, comprising a pressurizing device for pressurizing fluid by a compression stroke, a pressure detecting unit for detecting the maximum pressure value of the pressurizing device in each compression stroke and a central controller, wherein the central controller is used for driving the pressurizing device to return to a pre-pressing position from the end point of the compression stroke at a first displacement velocity, and then pressurizing the fluid from the pre-pressing position at a second displacement velocity slower than the first displacement velocity; and the pre-pressing position is a new start point of next compression stroke, and a pressure value is equal to the maximum pressure value of the former compression stroke. Therefore, on the premise of not influencing the pressurizing effect, and by using a mode of reducing the compression stroke, the detecting time is effectively shortened and the detecting benefit is improved greatly.
Description
Technical field
The present invention relates to a kind of ftercompction system, particularly relate to a kind of ftercompction system for high-performance liquid chromatograph.
Background technology
High performance liquid chroma-tography technology (High Performance Liquid Chromatography, HPLC) is most widely used a kind of chromatography in analytical technology, is mainly to utilize moving phase (mobile phase; Buffer solution or solvent) drive sample by quiet phase (the stationary phase of absorption use; Solid material or matrix), to flowing through the quiet different affinity that has mutually, thereby can reach the effect of separation due to compositions different in sample.Whereby, only need to see through the composition that a further Analyze ﹠ separate of light analyzer goes out, just can obtain required sample information, and be applicable to all kinds of sample analysis such as chemistry, chemical industry, pharmaceutical test, agriculture, environmental test, biochemistry, food and drink.
Due to sample by quiet analysis ability after mutually, can promote because the hold-up time (retention time) increases, therefore, quiet phase normally is filled in a chromatography pipe in tightly compacted mode, so, sample can not be smoothly by the chromatography pipe according to the gravity of itself, and the sample that must pressurize just can make the sample infiltration by this chromatography pipe.
Consult Fig. 1, take the pressue device 1 that generally is applied to high-performance liquid chromatograph (HPLC) as example, consist predominantly of a pedestal 11, a slide block 12, a screw rod 13, a pump 14, a stopper rod 15, and a motor 16.This slide block 12 slides against this pedestal 11 along an axis X direction.This screw rod 13 is hubbed on this pedestal 11 and spiral shell is worn this slide block 12 along this axis X direction.This pump 14 is arranged on this pedestal 11 1 sides, and have for sample into and out of entrance 141 export 142 with one.This stopper rod 15 slides on this pump 14 along this axis X direction, and with the fixed formation interlock of this slide block 12.This motor 16 is arranged on this pedestal 11 opposite sides, and with the fixed formation interlock of this screw rod 13.
When the entrance 141 of sample by this pump 14 enters in this pump 14, this motor 16 can drive this screw rod 13 rotations, make slide block 12 follow this screw rod 13 to drive this stopper rod 15 and be moved to the terminal point P1 of compression travel L by the starting point P0 of a compression travel L, the extruding sample is by the outlet 142 foregoing chromatography pipe (not shown) of infiltration of this pump 14.
only, in compression travel L each time, the pressure that 15 pairs of samples of this stopper rod produce, can diminish along with the space in this pump 14 and promote gradually, therefore, when this stopper rod 15 normally slowly marches to the application point P of contiguous terminal point P1 by starting point P0, could produce enough pressure extrusion sample permeation chromatography pipes, so, this stopper rod 15 is moved to a precompressed stroke L ' between this application point P gradually by the starting point P0 of compression travel L each time, normally do not produce the backlash of pressurization effect, under the situation that these stopper rod 15 velocity of displacements remain unchanged, obviously arranged the pressing time of delaying, reduce the disappearance of detection efficiency.
Summary of the invention
The objective of the invention is to shorten pressing time providing a kind of, and promote the ftercompction system of the high-performance liquid chromatograph of detection efficiency.
The ftercompction system of high-performance liquid chromatograph of the present invention comprises a pressue device, a pressure detection unit, and a central controller.This pressue device has a pump, and with limited compression travel slide in this pump and pressure fluid into and out of a stopper rod of this pump.This pressure detection unit is used for the detecting fluid at this stopper rod compression travel maximum pressure value that can produce each time.This central controller is connected with this pressure detection unit, and the maximum pressure value according to a front compression travel, drive this stopper rod by the terminal point of compression travel with one first velocity of displacement playback to a precompressed position, again by this precompressed position to be slower than one second velocity of displacement pressure fluid of this first velocity of displacement, this precompressed position is the ground zero of compression travel next time, and force value equates with the maximum pressure value of a front compression travel.
Effect of the present invention is the mode of reducing compression travel, effectively shortens detection time, reaches to promote the purpose that detects benefit.
Description of drawings
Fig. 1 is a schematic diagram, and the pressue device that generally is applied to high-performance liquid chromatograph (HPLC) is described;
Fig. 2 is a calcspar, and a preferred embodiment of the ftercompction system of the present invention's one high-performance liquid chromatograph is described;
Fig. 3 is a three-dimensional cutaway view of a pressue device in this preferred embodiment; And
Fig. 4 be in this preferred embodiment stopper rod by the three-dimensional cutaway view of a precompressed position towards a terminal point displacement.
Embodiment
The present invention is described in detail below in conjunction with drawings and Examples:
Consult Fig. 2, Fig. 3, ftercompction system one preferred embodiment of high-performance liquid chromatograph of the present invention is to be communicated with a chromatography pipe (not shown) of a high-performance liquid chromatograph (HPLC), comprise a pressue device 2, a pressure detection unit 3, and a central controller 4.
This pressue device 2 has a pedestal 21, a slide block 22, a screw rod 23, a pump 24, a stopper rod 25, reaches a motor 26 as actuator.This slide block 22 slides against this pedestal 21 along an axis X direction.This screw rod 23 is hubbed on this pedestal 21 and spiral shell is worn this slide block 22 along this axis X direction.This pump 24 is arranged on this pedestal 21 1 sides, and have for solvent into and out of entrance 241 export 242 with one.This stopper rod 25 slides on this pump 24 along this axis X direction, and with the fixed formation interlock of this slide block 22.This motor 26 is arranged on this pedestal 21 opposite sides, and with the fixed formation interlock of this screw rod 23.
This pressure detection unit 3 has a sensor 31 that is communicated with this pump 24 and is used for the detecting fluid pressure value, a converter 32 that analog signal is converted to digital signals, and is electrically connected to this sensor 31, this converter 32 and for a signal amplifier 33 of reinforcement aforementioned electric signal.
This central controller 4 has one and receives module 41, a control module 42, a control circuit 43, and a driving circuit 44.This receives module 41 and sees through this converter 32 and the mutual communication of this sensor 31.This controls rotating speed and driving force that module 42 sees through this control circuit 43 and this driving circuit 44 these motors 26 of control.
When the entrance 241 of solvent by this pump 24 enters in this pump 24, this central controller 4 can see through this control circuit 43 and drive this screw rod 23 rotations of these motor 26 drives with this driving circuit 44, make slide block 22 follow these screw rod 23 displacements and drive this stopper rod 25 to be moved to the terminal point P1 of compression travel L by the starting point P0 of a compression travel L, the extruding solvent is by the outlet 142 permeation chromatography pipe (not shown) of this pump 14.
importantly, at this stopper rod 25 each time during pressurized solvent, the maximum pressure value that the sensor 31 of this pressure detection unit 3 can these stopper rods 25 of detecting can produce at compression travel L each time, convert analog signal to digital signals through this converter 32, and after strengthening the aforementioned electric signal with this signal amplifier 33, return to the reception module 41 of this central controller 4, at this moment, this controls module 42 can be according to the maximum pressure value of a front compression travel L, see through this control circuit 43 and this driving circuit 44 and control rotating speed and the driving force of these motors 26, this motor 26 is revved up, and drive this stopper rod 25 with one first velocity of displacement fast offset, terminal point P1 by compression travel playbacks to a precompressed position P immediately through starting point P0, and the judgement of this precompressed position P is to continue to detect by this sensor 31 force value that produces in these stopper rod 25 displacement processes, when equating with the maximum pressure value of a front compression travel L to force value, represent that namely this stopper rod 25 has arrived this precompressed position P, and as the ground zero of compression travel L ' next time.
then, this central controller 4 can be controlled these motors 26 rotating speed that slows down, and drive this stopper rod 25 with one second velocity of displacement that is slower than this first velocity of displacement by this precompressed position P towards terminal point P1 position displacement, and synchronous pressurized solvent, the extruding solvent is by the outlet 242 foregoing chromatography pipe (not shown) of infiltration of this pump 24, at this moment, the maximum pressure value that is produced by these stopper rod 25 pressurized solvents might equal or be not equal to a front maximum pressure value, only, this sensor 31 can continue the force value that repayment is detected, by this central controller 4 this stopper rod 25 of judgement precompressed position P each time, and then reach the purpose that shortens compression travel L.
According to the above as can be known, the ftercompction system of high-performance liquid chromatograph of the present invention has following advantages and effect:
The present invention is under the front topic that does not affect the pressurization effect, mode take reduction compression travel L as compression travel L ', shorten the time of backlash, and this compression travel L ' can coordinate each time the required force value of pressurized solvent to revise, the precompressed position P that this stopper rod 25 is returned back to rapidly can produce effective compressed action, not only can accelerate compression motion, effectively shorten detection time, and can significantly promote the detection benefit.
Claims (4)
1. the ftercompction system of a high-performance liquid chromatograph, is characterized in that, it comprises:
A pressue device, have a pedestal, pump, with limited compression travel slide in this pump and pressure fluid into and out of a stopper rod of this pump, slide against this pedestal and with a slide block of the fixed formation interlock of this stopper rod, be hubbed on this pedestal and spiral shell is worn screw rod of this slide block along this axis direction along an axis direction, and form interlock with this screw rod and drive an actuator of this stopper rod motion;
A pressure detection unit is used for the detecting fluid at this stopper rod compression travel maximum pressure value that can produce each time; And
A central controller, be electrically connected to this pressure detection unit, this actuator, and the maximum pressure value according to a front compression travel, drive this stopper rod by the terminal point of compression travel with one first velocity of displacement playback to a precompressed position, again by this precompressed position to be slower than one second velocity of displacement pressure fluid of this first velocity of displacement, this precompressed position is the ground zero of compression travel next time, and force value equates with the maximum pressure value of a front compression travel.
2. the ftercompction system of high-performance liquid chromatograph as claimed in claim 1, is characterized in that, this actuator is a motor.
3. the ftercompction system of high-performance liquid chromatograph as claimed in claim 1, it is characterized in that, this pressure detection unit has a sensor that is communicated with this pump and is used for the detecting force value, a converter that analog signal is converted to digital signals, and a signal amplifier that is electrically connected to this sensor, this converter.
4. the ftercompction system of high-performance liquid chromatograph as claimed in claim 3, it is characterized in that, this central controller has one and receives module, a control module, a control circuit, and driving circuit, this receives module and sees through this converter and the mutual communication of this sensor, and this is controlled module and is used for controlling rotating speed and the driving force of this actuator through this control circuit and this driving circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910205151 CN102043029B (en) | 2009-10-16 | 2009-10-16 | Repressing system of high performance liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910205151 CN102043029B (en) | 2009-10-16 | 2009-10-16 | Repressing system of high performance liquid chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102043029A CN102043029A (en) | 2011-05-04 |
| CN102043029B true CN102043029B (en) | 2013-06-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910205151 Expired - Fee Related CN102043029B (en) | 2009-10-16 | 2009-10-16 | Repressing system of high performance liquid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102043029B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101059486A (en) * | 2007-05-10 | 2007-10-24 | 复旦大学 | Hot expansion continuous microflow high pressure gradient pump for high-performance liquid chromatographer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6997683B2 (en) * | 2003-01-10 | 2006-02-14 | Teledyne Isco, Inc. | High pressure reciprocating pump and control of the same |
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2009
- 2009-10-16 CN CN 200910205151 patent/CN102043029B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101059486A (en) * | 2007-05-10 | 2007-10-24 | 复旦大学 | Hot expansion continuous microflow high pressure gradient pump for high-performance liquid chromatographer |
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| Publication number | Publication date |
|---|---|
| CN102043029A (en) | 2011-05-04 |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130619 Termination date: 20191016 |