CN102031303A - Method for detecting and evaluating environmental risks caused by transgene escape - Google Patents
Method for detecting and evaluating environmental risks caused by transgene escape Download PDFInfo
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Abstract
The invention belongs to the technical field of environmental biological safety detection and analysis, and particularly relates to a method for detecting and evaluating environmental risks caused by transgene escape. Insect-resistant transgenic rice, non-transgenic rice and wild allied population are used as analysis materials. The method comprises the following steps of: obtaining hybrid F1 populations containing transgenes and containing no transgenes and selfing separation F2 generation populations containing transgenic hybrid F1 generation by combination and artificial crossing of the analysis materials; planting the F1 populations and the F2 separation populations in fields under the environments with insect pressure or without insect pressure, and measuring the fitness related traits of the populations; and calculating the environmental risks (percent) of the escaping transgenes to the wild allied population by comparing and analyzing the fitness benefit and fitness cost of the transgenic populations. The method detects the environmental risks of the transgenes under controlled conditions, has accurate detection result and high efficiency, and has good application prospect on the environmental safety guarantee of commercialized production of the transgenic rice.
Description
Technical field
The invention belongs to environmental organism security detection and analysis technical field, be specifically related to the detection evaluation method of the environmental risk that a kind of transgene escape causes.
Background technology
Developing rapidly of transgenic technology, cultivation and the plantation of transgenic plant have greatly been promoted, the commercializations of transgenic plant is used, for the raising of crop yield, reduce agricultural chemicals use, improve agroecological environment and the grain security problem has been brought new opportunity.The High-Interest Object that the locked genetically modified organism technology of China is future development will have more transgenic plant breed of variety to come out.Yet, detection and evaluation that the huge bottleneck that transgenic plant commercialization plantation faces is exactly an environmental organism safety, particularly genetically modified escape and the environmental risk that may bring thereof, being transgenosis escapes into after the wild nearly edge species by genetic drift, may cause a series of consequences such as local in imminent danger and extinction of serious weeds problem and wild relatives colony.The existence of this environmental risk has seriously restricted the decision-making of transgenic crop commercialization plantation.Therefore, before the commercialization plantation, must the latency environment risk that transgene escape brought be detected, with safety and the sustainable use of guaranteeing transgenic plant.
Transgenic plant are discharged into after the environment, and its foreign gene will escape into the wild near edge species of transgenic plant by genetic drift, whether has the potential environmental risk for transgene escape, and the degree of its risk all must detect and estimate.But, up to the present, have only the relatively more effective detection method of genetic drift frequency detecting, and for whether bringing the degree of environmental risk and risk still to lack effective detection method after the genetic drift escape.
Summary of the invention
The purpose of this invention is to provide a kind of method whether transgenosis of escaping causes environmental risk of estimating that detects.
Whether transgene escape can bring environmental risk, this depends on to a great extent whether this gene causes that wild nearly edge species fitness (refers under the certain condition environment, organism can survive and transmit the ability that its gene is given the offspring) change largely, when having to increase (fitness interests) largely or reduce (fitness cost), fitness all may cause different environmental risks.The present invention utilizes transgenosis wild near edge species colony to plant under artificial controlled different experimental conditions to bring the crucial proterties of fitness " interests " and " cost " variation to analyze, whether cause the degree of environmental risk and risk to detect to transgene escape, to reach the purpose of transgenic plant safety plantation.
The detection evaluation method of the environmental risk that the transgene escape that the present invention proposes causes, concrete steps comprise: (1) makes up and contains transgenosis and do not contain the wild nearly edge species experimental population of transgenosis; (2) be designed for the field planting experiment that transgene escape causes environmental risk to detect; (3) set up detection and the judgement criteria that transgene escape causes environmental risk.Wherein:
Described structure contains transgenosis and does not contain the wild nearly edge species experimental population of transgenosis, is according to certain array mode, utilizes artificial hybridization to obtain the hybrid F of target transgenic plant and wild nearly edge species
1In generation, separated F with selfing
2Realize for experimental population.Concrete method is:
Select target transgenic plant and non-transgenic parental breed thereof are hybridized with the individuality of wild nearly edge species respectively, obtain to contain the hybrid F of genetically modified transgenic plant-wild species
1Colony (A) and do not contain the hybrid F of genetically modified parental breed-wild species
1Colony (B); By to containing genetically modified hybrid F
1Carry out the bagging selfing, obtain respectively to contain transgenosis and do not contain genetically modified F
2For segregating population; Utilize the primer of target transgenosis design, to F
2Carry out pcr amplification for segregating population, identify and contain genetically modified F
2Colony (C) and do not contain genetically modified F
2Colony (D).
According to the genetically modified hybrid F that contains that aforesaid method obtained
1Colony (A), do not contain genetically modified hybrid F
1Genetically modified selfing F is contained in colony (B)
2Segregating population (C) and do not contain genetically modified selfing F
2Segregating population (D) carries out fitness analysis experiment under the controlled experiment condition.
The described transgene escape that is designed for causes the field planting of environmental risk detection to be tested, be A, the B that will obtain, C, D experimental population respectively by plantation spacing in the rows and the line-spacing of 20cm * 20cm or 40cm * 40cm, will contain transgenosis and not contain genetically modified A and B colony or C and D colony carry out single cropping and mixed planting in a sub-district.Be the single cropping pattern of transfer-gen plant, the single cropping pattern of non-transgenic plant, the alternate mixed planting pattern of transfer-gen plant and non-transgenic plant is seen Fig. 1-shown in Figure 3.
According to genetically modified type or function, controlled experimental situation (for example: the environment that is set to target pest and no target pest worm pressure at the insect-resistant transgenic plants environment) is designed to selection environment of pressing and the environment that does not have the pressure selected respectively, above-mentioned experimental population is planted respectively in the environment that the pressure selected the is arranged environment that selection is pressed with nothing, and, carry out the measurement of fitness correlated character respectively in experimental population plant each growth phase of the life history.
Be used to detect required form of measuring of ecological fitness and economical character, mainly contain plant height (cm), individual plant tiller number (individual), single-strain tassel number (individual), single-strain seed output (grain), thousand seed weight (g), seed germination rate (%), seedling rate, or the like, specifically should be selected according to the practical situation of detection plant.
Describedly set up detection and the judgement criteria that transgene escape causes environmental risk, promptly set up compound fitness index, estimate the degree of environmental risk (%):
The fitness of each morphological characters is: contain the observed value of transfer-gen plant/do not contain observed value * 100% of transfer-gen plant.
Compound fitness is: the mean number of the fitness of each morphological characters.
Estimate transgene escape by the value (%) of compound fitness and cause environmental risk (%).
The following fitness correlated character of concrete selection is measured and is analyzed:
According to each handles comparative analysis (A: B and the C: D) that contains genetically modified experimental population and do not contain the measured data of transgenic experiments colony in the controlled experiment, detect fitness interests (WB) or fitness cost (WC) that transgenosis is brought wild nearly edge species, and determine the environmental risk that transgene escape causes on this basis.The environmental risk of the high more then transgene escape of fitness intensity of variation that experimental population is brought as transgenosis is high more, on the contrary the low or devoid of risk of risk then.
The calculation formula of fitness interests (WB) or fitness cost (WC) is:
W=(transfer-gen plant observed value-non-transgenic plant observed value)/non-transgenic plant observed value ... (1)
The value of W changes between-1～1, when
WValue on the occasion of, there are the fitness interests in expression, when
WThere is the fitness cost in value for negative value, expression, when
WValue is 0 or approaches 0 that expression does not have fitness to change.
Compound fitness index is the mean value of each proterties fitness, and calculation formula is:
W comp?=?∑
W i-j ?/N …………………………………………………………………(2)
Wherein i-j is different fitness shape, and N is for measuring the number of proterties.
The present invention is a kind of method of utilizing fitness analyzing and testing transgene escape to bring environmental risk to the farm crop wild relatives.It is analysis of material that the present invention specifically utilizes pest-resistant transgenic rice and non-transgenic paddy rice and wild relatives (wild-rice/weeds rice) colony thereof, carry out artificial hybridization with wild-rice and weeds rice respectively with transgenic paddy rice and non-transgenic parent, obtain to contain transgenosis and do not contain genetically modified hybrid F
1Colony and contain transgenosis hybrid F
1The selfing in generation separates F
2For colony.To contain transgenosis and not contain genetically modified hybrid F
1Colony and F
2Segregating population carries out field planting under the environment that has worm to press and do not have worm to press, and to the fitness correlated character of each colony: plant height, individual plant tiller number, single-strain tassel number, the solid number of individual plant and thousand seed weight are measured.By the fitness cost (WC) of comparative analysis transgenosis colony under fitness interests (WB) that transgenosis colony under the worm pressure ring border is arranged and no worm pressure ring border, the environmental risk (%) that the transgenosis that calculating is escaped is brought wild relatives.The present invention detects genetically modified environmental risk under controlled condition, detected result is accurate, efficient is high, and the environmental safety of producing for the transgenic paddy rice commercialization guarantees to have a good application prospect.
Description of drawings
Fig. 1 be transfer-gen plant (
*) the single cropping pattern.
Fig. 2 be the non-transgenic plant (
Zero) the single cropping pattern.
Fig. 3 is the alternate mixed planting pattern of transfer-gen plant and non-transgenic plant.
Embodiment
Embodiment 1:
Utilize method of the present invention to detect the environmental risk that pest-resistant transgenic rice Bt gene escapes into weeds rice colony.Experiment material is as shown in table 1.
The constructing environment Biosafety detects contain transgenosis and do not contain the step of transgenosis wild relatives hybrid experimental population as follows:
(1) determine cross combination:
A. transgenic paddy rice * wild-rice,
B. non-transgenic parental rice * wild-rice,
C. transgenic paddy rice * weeds rice,
D. non-transgenic paddy rice * weeds rice;
(2), obtain the hybrid F that contains transgenosis (cross combination A and C) and do not contain transgenosis (cross combination B and D) respectively by artificial hybridization
1Colony;
(3) to making up the hybrid F of A and C
1Colony carries out bagging selfing and genetically modified detection, identifies F
2Whether the individuality of segregating population comprises transgenosis;
(4) obtain to contain genetically modified F respectively
2Segregating population (+) and do not contain genetically modified F
2Segregating population (-).
Utilize the artificial hybridization method, with transgenosis cultivated rice strain MF1 and non-transgenic cultivated rice parent bright extensive 86 be male parent (♂) respectively with maternal W1 of weeds rice or W2(♀) hybridize, obtain to contain
BtThe F of insect-resistant transgenic
1For hybrid (MF1/W1 and MF1/W2) with do not contain
BtThe F of insect-resistant transgenic
1Hybrid (M86/W1 and M86/W2).Simultaneously, to containing genetically modified F
1Hybrid carries out the bagging selfing, obtains selfing F
2For segregating population, utilize according to insect-resistant transgenic (
Bt) Auele Specific Primer of dna sequence dna design is right, to F
2Segregating population carries out PCR to be identified, everyly contains genetically modified plant and is accredited as
Bt(+) do not contain genetically modified plant and is accredited as
Bt(-).
When field experiment, be provided with 2 and contain
BtThe F of gene
1Generation, 2 F that do not contain the Bt gene
1Generation, 2 F
2For Bt (+) colony and 2 F
2For single kind of pattern of Bt (-) colony, 4 F
1Generation and F
2Generation
Bt(+) and
Bt(-) colony uniform mixing competition species implant model.Therefore this experiment comprises 12 kinds of processing altogether, and every kind of processing is provided with 4 repetitions (48 sub-districts).Each sub-district plantation 8 row * 8 avenue system strain, strain spacing 20cm.The scheme of sub-district completely random (district's group) is carried out field layout and plantation.This experiment is carried out in two different worm pressure ring borders: the extremely low worm pressure ring border and 2 of 1) spraying chemical pesticide) the natural worm pressure ring border of not using any chemical pesticide by routine.In experiment material different breeding time, respectively single-strain tassel number, single fitness proterties such as seed number, thousand seed weight and seed germination rate of planting after the individual plant tiller number of jointing stage, the plant height in ripening stage, the plant maturation are measured, promptly select following proterties:
(1) plant height (cm): from the ground of plant to the height of long shoot leaf (fringe);
(2) individual plant tiller number (individual): all of each single plant sum totals of tillering;
(3) single-strain tassel number (individual): the spike number sum total that each single plant is all;
(4) single-strain seed output (grain): the full fruit number sum total of all of each single plant;
(5) thousand seed weight (g): the weight of 1000 seeds;
(6) seed germination rate (%): germinating seed number/be used to sprout seed number * 100% of experiment;
(7) seedling rate (%): seedling number/be used to sprout seed number * 100% of experiment.
The result is as shown in table 2.From separate analysis to each fitness proterties, in low worm pressure ring border, contain genetically modified material and do not contain genetically modified material basically identical all on each proterties, the minority proterties is only arranged, and there were significant differences, insect-resistant transgenic is described under the situation that does not have worm to press, to the almost not influence of fitness of weeds rice colony.And in high worm pressure ring border, the fitness character value that contains transgenic line will be than non-transgenic material height, especially on the single-strain seed yield traits, present significant difference, it can be said that bright insect-resistant transgenic can bring the advantage of fitness to the weeds rice under high worm press strip spare.
And adopt compound fitness index method, the value that will contain each proterties of transgenic line fitness earlier changes into the ratio (W value) that increases or reduce with respect to the non-transgenic material, investigates the influence (table 3) of insect-resistant transgenic to fitness by calculating compound fitness index.The external source of transform insect-resistant gene paddy rice
BtGene escapes into after the weeds rice, contains transgenosis F
1And F
2Colony is in the environment that low worm is pressed, except do not contain genetically modified F than it on single-strain tassel number
1And F
2Colony wants beyond the height, and other fitness proterties does not all have unified trend, contains genetically modified F
1Generation and F
2Compound fitness index under Dai Zaidan kind and the miscegenation condition is also only high by 5.5% than the latter's compound fitness, 1.8%, 5.0% and 4.8%, this shows and contains genetically modified hybrid F
1And F
2Colony does not contain the fitness basically identical (＜5%) of genetically modified colony with it in the environment that low worm is pressed.And in the environment that high naturally worm is pressed, contain genetically modified F
1And F
2Colony on the important single-strain seed yield traits than its non-transgenic colony height far away beyond, therefore other fitness proterties also all is improved to some extent, and contains genetically modified F
1Generation and F
2The Dai Zaigao worm is pressed single plant and the miscegenation condition under compound fitness index also high by 10.8% than the latter's compound fitness, 7.7%, 22.0% and 12.0%, this shows and contains genetically modified hybrid F
1And F
2The fitness of colony does not contain genetically modified colony (〉 10% far above it in the environment that high worm is pressed).Show that also containing genetically modified segregating population does not contain genetically modified colony than it and have higher existence and competitive edge.Further infer, change pest-resistant Bt gene and escape into weeds rice colony, can under damaged by vermin situation, cause the more vigorous growth of weeds rice, bring certain environmental risk.
Embodiment 2:
Utilize the inventive method to detect the antiweed transgenic paddy rice
AroaGene escapes into the environmental risk of common wild-rice.Experiment material is as shown in table 4.
Utilize the method for artificial hybridization, with antiweed transgenic paddy rice MA and non-transgenic paddy rice M86 be male parent (♂) respectively with common wild-rice W2(as maternal ♀) hybridize, obtain to include the genetically modified F of antiweed
1(W2-MA) and not contain the genetically modified F of antiweed
1(W2-M86) hybrid population.
When carrying out field experiment, the F that contains antiweed is set
1The single F that plants, do not contain antiweed of colony (W2-MA)
1The processing of single kind of colony (W2-M86) and W2-MA and W2-M86 uniform mixing competition plantation is planted in the environment of not spraying herbicide and spraying herbicide.Experiment comprises that 6 kinds of processing, each processing comprise 4 repetitions (totally 24 sub-districts), each sub-district plantation 8 row * 8 avenue system strain, strain spacing 40cm.The scheme of sub-district completely random (district's group) is carried out field layout and plantation.In experiment material different breeding time, respectively single-strain tassel number, single fitness proterties such as seed number, thousand seed weight and seed germination rate of planting after the individual plant tiller number of jointing stage, the plant height in ripening stage, the plant maturation are measured, the result is as shown in table 4.
From the separate analysis to each fitness proterties, in the environment of spraying herbicide, because the non-transgenic paddy rice is subjected to weedicide influence, great majority can not normal growth and growth, and are very low in the reproduction proterties; Transgenic line is then unaffected substantially, and both field performance difference is huge.And in not having the environment of spraying herbicide, anti-herbicide gene still shows certain fitness advantage, and this is mainly reflected on individual plant tiller number, single-strain tassel number and the single-strain seed number (table 5).
And adopt compound fitness method to containing genetically modified F1 for colony with when not containing genetically modified F1 and carrying out comprehensive assessment for colony, find to be contained under the situation of using weedicide genetically modified F
1For the fitness of colony single plant and the miscegenation pattern under will compared to the fitness that does not contain transgenosis colony distinguish high 172.9% and 255.3%(table 6), this shows antiweed
AroaSelect to press if gene runs into weedicide after escape, huge fitness advantage will be provided.And do not using under the weedicide situation, the multiplicity index of transgenic line is 8.8% and 12.0% with respect to the non-transgenic material, shows
AroaGene escape into do not have in the environment that weedicide uses after, also, cause corresponding ecological result for wild-rice colony raising phase fitness.
The experiment material that is comprised among table 1, the embodiment 1.
| Sequence number | Title material | The source | Characteristic |
| 1 | Transgenic paddy rice strain (MF1) | Fujian China | Cultivated rice |
| 2 | The bright extensive 86(M86 of non-transgenic parent) | Fujian China | Cultivated rice |
| 3 | The weeds rice W1 of colony | China Hainan | The weeds rice |
。
The no worm of table 2 is pressed and has in the worm pressure ring border, the fitness proterties of each experiment material and contain transgenic line and non-transgenic between comparative result.Wherein statistically significant (p＜0.05) is compared in runic band " * " expression with the non-transgenic material, the runic band "
" expression compares statistics clean significantly (p＜0.1) with non-transgenic cross combination.
Table 3, according to table 2 result, calculate each experiment and handle down and contain each fitness proterties of transgenic line compared to W value that does not contain transgenic line and compound fitness index thereof.
The experiment material that is comprised among table 4, the embodiment 2
| Sequence number | Title material | The source | Characteristic |
| 1 | Antiweed transgenic paddy rice strain (MA) (contains AroaGene) | Fujian China | Cultivated rice |
| 2 | The bright extensive 86(M86 of non-transgenic parent) | Fujian China | Cultivated rice |
| 3 | Wild-rice colony (W2) | Chinese Guangdong | Wild-rice |
Table 5, use and do not use contain transgenosis in the weedicide environment and do not contain each fitness character value of transgenosis wild-rice colony and contain transgenic line and non-transgenic between comparative result.Wherein statistically significant (p＜0.05) is compared in runic band " * " expression with the non-transgenic material.
Table 6, according to table 5 result, calculate each experiment and handle down and contain each fitness proterties of transgenic line compared to W value that does not contain transgenic line and compound fitness index thereof.
Claims (4)
1. the detection evaluation method of the environmental risk that causes of a transgene escape, it is characterized in that concrete steps are: (1) makes up and contains transgenosis and do not contain the wild nearly edge species experimental population of transgenosis; (2) be designed for the field planting experiment that transgene escape causes environmental risk to detect; (3) set up detection and the judgement criteria that transgene escape causes environmental risk; Wherein:
Described structure contains transgenosis and does not contain the wild nearly edge species experimental population of transgenosis, is according to certain array mode, utilizes artificial hybridization to obtain the hybrid F of target transgenic plant and wild nearly edge species
1In generation, separated F with selfing
2Realize that for experimental population concrete steps are:
Select target transgenic plant and non-transgenic parental breed thereof are hybridized with the individuality of wild nearly edge species respectively, obtain to contain the hybrid F of genetically modified transgenic plant-wild species
1Colony (A) and do not contain the hybrid F of genetically modified parental breed-wild species
1Colony (B); By to containing genetically modified hybrid F
1Carry out the bagging selfing, obtain respectively to contain transgenosis and do not contain genetically modified F
2For segregating population; Utilize the primer of target transgenosis design, to F
2Carry out pcr amplification for segregating population, identify and contain genetically modified F
2Colony (C) and do not contain genetically modified F
2Colony (D);
According to the genetically modified hybrid F that contains that aforesaid method obtained
1Colony (A), do not contain genetically modified hybrid F
1Genetically modified selfing F is contained in colony (B)
2Segregating population (C) and do not contain genetically modified selfing F
2Segregating population (D) carries out fitness analysis experiment under the controlled experiment condition;
The described transgene escape that is designed for causes the field planting of environmental risk detection to be tested, be A, the B that will obtain, C, D experimental population respectively by plantation spacing in the rows and the line-spacing of 20cm * 20cm or 40cm * 40cm, will contain transgenosis and not contain genetically modified A and B colony or C and D colony carry out single cropping and alternate mixed planting in a sub-district; Promptly be divided into 3 kinds of cropping patterns: the single cropping pattern of transfer-gen plant, the single cropping pattern of non-transgenic plant, the alternate mixed planting pattern of transfer-gen plant and non-transgenic plant;
According to genetically modified type or function, controlled experimental situation is designed to select the environment of pressing respectively and does not have the environment that selection is pressed, above-mentioned experimental population is planted respectively in the environment that the pressure selected the is arranged environment that selection is pressed with nothing, and, carry out the measurement of fitness correlated character respectively in experimental population plant each growth phase of the life history;
Describedly set up detection and the judgement criteria that transgene escape causes environmental risk, promptly set up compound fitness index, estimate the degree of environmental risk, represent with %:
The fitness of each morphological characters is: contain the observed value of transfer-gen plant/do not contain observed value * 100% of transfer-gen plant;
Compound fitness is: the mean number of the fitness of each morphological characters;
Estimate transgene escape by the value (%) of compound fitness and cause environmental risk (%).
2. the detection evaluation method of the environmental risk that transgene escape according to claim 1 causes is characterized in that describedly according to certain array mode, utilizes artificial hybridization to obtain the hybrid F of target transgenic plant and wild nearly edge species
1In generation, separated F with selfing
2Realize that for experimental population concrete steps are:
(1) determine cross combination:
A. transgenic paddy rice * wild-rice;
B. non-transgenic parental rice * wild-rice;
C. transgenic paddy rice * weeds rice;
D. non-transgenic paddy rice * weeds rice;
(2), obtain to contain transgenosis respectively and comprise cross combination A and C, and do not contain the hybrid F that transgenosis comprises cross combination B and D by artificial hybridization
1Colony;
(3) to making up the hybrid F of A and C
1Colony carries out bagging selfing and genetically modified detection, identifies F
2Whether the individuality of segregating population comprises transgenosis;
(4) obtain to contain genetically modified F respectively
2Segregating population (+) and do not contain genetically modified F
2Segregating population (-).
3. the detection evaluation method of the environmental risk that transgene escape according to claim 1 causes is characterized in that described fitness correlated character selection is as follows:
Plant height, cm: from the ground of plant to the height of long shoot leaf or fringe;
The individual plant tiller number, individual: all of each single plant sum totals of tillering;
Single-strain tassel number, individual: the spike number sum total that each single plant is all;
Single-strain seed output, grain: the full fruit number sum total of all of each single plant;
Thousand seed weight, the weight of g:1000 grain seed;
Seed germination rate, %: germinating seed number/be used to sprout seed number * 100% of experiment;
Seedling rate, %: seedling number/be used to sprout seed number * 100% of experiment.
4. the detection evaluation method of the environmental risk that transgene escape according to claim 1 causes is characterized in that the following fitness correlated character of described concrete selection measures and analyze:
According to each handles the comparative analysis that contains genetically modified experimental population and do not contain the measured data of transgenic experiments colony in the controlled experiment, detect fitness interests or fitness cost that transgenosis is brought wild nearly edge species, and determine the environmental risk that transgene escape causes on this basis; The environmental risk of the high more then transgene escape of fitness intensity of variation that experimental population is brought as transgenosis is high more, on the contrary the low or devoid of risk of risk then;
The calculation formula of fitness interests or fitness cost is:
W=(transfer-gen plant observed value-non-transgenic plant observed value)/non-transgenic plant observed value ... (1)
The value of W changes between-1～1, when
WValue on the occasion of, there are the fitness interests in expression, when
WThere is the fitness cost in value for negative value, expression, when
WValue is 0 or approaches 0 that expression does not have fitness to change;
Compound fitness index is the mean value of each proterties fitness, and calculation formula is:
W comp?=?∑
W i-j ?/N …………………………………………………………………(2)
Wherein i-j is different fitness shape, and N is for measuring the number of proterties.
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| CN103563739A (en) * | 2013-11-22 | 2014-02-12 | 复旦大学 | Detecting and evaluating method for environmental risk caused by transgene escape of herbicide-resistant rice |
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| CN108796034A (en) * | 2018-06-25 | 2018-11-13 | 复旦大学 | A kind of comparison hybrid population sampling method for establishing transgenosis drift environment safety detection |
| CN111139311A (en) * | 2020-01-17 | 2020-05-12 | 南京农业大学 | Evaluation method and application of transgenic rice weediness gene drift risk |
| CN111387050A (en) * | 2020-05-08 | 2020-07-10 | 南京农业大学 | Method for evaluating fitness of filial generation of transgenic soybean and wild soybean in hybridization or backcross |
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| CN103563739B (en) * | 2013-11-22 | 2015-12-09 | 复旦大学 | A kind of rice herbicide resistant transgene escape causes the method for determination and evaluation of environmental risk |
| CN103858629A (en) * | 2014-02-14 | 2014-06-18 | 中国农业大学 | Method for evaluating influence of entomophilous insects on gene drift of transgenic crops |
| CN108796034A (en) * | 2018-06-25 | 2018-11-13 | 复旦大学 | A kind of comparison hybrid population sampling method for establishing transgenosis drift environment safety detection |
| CN108796034B (en) * | 2018-06-25 | 2021-04-30 | 复旦大学 | Comparative hybrid population sampling method for establishing safety detection of transgenic drift environment |
| CN111139311A (en) * | 2020-01-17 | 2020-05-12 | 南京农业大学 | Evaluation method and application of transgenic rice weediness gene drift risk |
| CN111387050A (en) * | 2020-05-08 | 2020-07-10 | 南京农业大学 | Method for evaluating fitness of filial generation of transgenic soybean and wild soybean in hybridization or backcross |
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