CN102030837A - Method for purifying and concentrating zymotic fluid of sodium hyaluronate - Google Patents
Method for purifying and concentrating zymotic fluid of sodium hyaluronate Download PDFInfo
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- CN102030837A CN102030837A CN2009102047270A CN200910204727A CN102030837A CN 102030837 A CN102030837 A CN 102030837A CN 2009102047270 A CN2009102047270 A CN 2009102047270A CN 200910204727 A CN200910204727 A CN 200910204727A CN 102030837 A CN102030837 A CN 102030837A
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- purifying
- hyaluronate sodium
- fermented liquid
- concentrates
- sodium hyaluronate
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Abstract
The invention discloses a method for purifying and concentrating zymotic fluid of sodium hyaluronate, which is characterized in that the zymotic fluid of sodium hyaluronate is firstly purified to remove impurities by resin adsorption and air agitation, and then is concentrated by adding salt and ethanol, and finally, the protein content of sodium hyaluronat precipitate does not exceed 0.05, the endotoxin does not exceed 0.01Eu, the clarity of solution is not lower than 99 percent, and the sodium hyaluronate is highly purified. At the same time, more than 50 percent of the ethanol can be saved, and the method for purifying and concentrating zymotic fluid of sodium hyaluronate has the advantages that the cost is lowered, and the product is improved.
Description
Affiliated technical field
The present invention relates to a kind of purifying and concentrate hyaluronate sodium fermented liquid method, belong to technical field of bioengineering.
Background technology:
In the fermented liquid that produces in China's fermentative production hyaluronate sodium process, the hyaluronic acid sodium content is about 0.3% at present.Usually extracting method is that fermented liquid is input in 3 times the ethanol, makes hyaluronate sodium produce the flocculence precipitation, but also many impurity and intracellular toxin is also got off with hyaluronate sodium is precipitated simultaneously.Protein content≤0.1 in the spissated hyaluronate sodium throw out of traditional method purifying according to surveying and determination, intracellular toxin≤0.5Eu, clarity of solution 〉=98% is for later filtration causes very big difficulty.
The invention provides a kind of purifying and concentrate hyaluronate sodium fermented liquid method, use protein content≤0.05 in the spissated hyaluronate sodium throw out of this method purifying, intracellular toxin≤0.01Eu, clarity of solution 〉=99%, greatly purifying hyaluronate sodium.Simultaneously can save ethanol 50% above consumption, save cost, improve the quality of product.
Summary of the invention
The present invention provides a kind of purifying and concentrates hyaluronate sodium fermented liquid method, it is characterized in that the hyaluronate sodium fermented liquid is removed the impurity purifying through carrying out resin absorption and pneumatic blending, concentrate with salt and ethanol, make protein content in the hyaluronate sodium throw out≤0.05 at last, intracellular toxin≤0.01Eu, clarity of solution 〉=99%, greatly purifying hyaluronate sodium.Simultaneously can save ethanol 50% above consumption, save cost, improve product.
Technical scheme:
The present invention provides a kind of purifying and concentrates hyaluronate sodium fermented liquid method, it is characterized in that the hyaluronate sodium fermented liquid is input to a stainless steel reaction jar, remove the impurity purifying through resin absorption and pneumatic blending, concentrate with salt and ethanol again, make protein content in the hyaluronate sodium throw out≤0.05 at last, intracellular toxin≤0.01Eu, clarity of solution 〉=99%, greatly purifying hyaluronate sodium.Simultaneously can save ethanol 50% above consumption, save cost, improve product.
The invention has the advantages that:
A kind of purifying provided by the invention concentrates hyaluronate sodium fermented liquid method, the hyaluronate sodium fermented liquid is removed the impurity purifying through being input into capable resin absorption and pneumatic blending earlier, concentrate with salt and ethanol again, make protein content in the hyaluronate sodium throw out≤0.05 at last, intracellular toxin≤0.01Eu, clarity of solution 〉=99%, greatly purifying hyaluronate sodium.Simultaneously can save ethanol 50% above consumption, save cost, improve product.
Embodiment:
Enforcement of the present invention is like this: the resin that will clean is filled into above the interior stainless steel filtering net of stainless steel reaction jar, and loading level is the 1/4-1/5 of tank volume; Inject the hyaluronate sodium fermented liquid, temperature remains on 40-60 ℃, feeds filtered air, carries out pneumatic blending, makes resin absorption 4-8 hour, the mother liquor of draining; Put into pure water again, resin several in the purge tank, bubbling air stirs simultaneously, quickens cleaning speed, washes a large amount of impurity off; Bleed off washing water then, add 5-15% salt solution, pneumatic blending 3-5 hour; As many again again 95% ethanol concentrates, and it is standby to collect concentrated solution for washing.The continuous washing secondary is collected the merging concentrated solution for washing and is entered next procedure.
Claims (4)
1. the present invention provides a kind of purifying and concentrates hyaluronate sodium fermented liquid method, it is characterized in that the hyaluronate sodium fermented liquid is input to a stainless steel reaction jar, remove the impurity purifying through resin absorption and pneumatic blending, concentrate with salt and ethanol, make protein content in the hyaluronate sodium throw out≤0.05 at last, intracellular toxin≤0.01Eu, clarity of solution 〉=99%, greatly purifying hyaluronate sodium.
2. a kind of purifying as claimed in claim 1 concentrates hyaluronate sodium fermented liquid method, it is characterized in that the resin loading level is the 1/4-1/5 of tank volume.。
3. a kind of purifying as claimed in claim 1 concentrates hyaluronate sodium fermented liquid method, it is characterized in that injecting the hyaluronate sodium fermented liquid after, temperature remains on 40-60 ℃; The line space of going forward side by side gas mixing was mixed 4-8 hour.
4. a kind of purifying as claimed in claim 1 concentrates hyaluronate sodium fermented liquid method, it is characterized in that adding brinish concentration 5-15%, and the line space of going forward side by side gas mixing was mixed 3-5 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102047270A CN102030837A (en) | 2009-09-27 | 2009-09-27 | Method for purifying and concentrating zymotic fluid of sodium hyaluronate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102047270A CN102030837A (en) | 2009-09-27 | 2009-09-27 | Method for purifying and concentrating zymotic fluid of sodium hyaluronate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102030837A true CN102030837A (en) | 2011-04-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102047270A Pending CN102030837A (en) | 2009-09-27 | 2009-09-27 | Method for purifying and concentrating zymotic fluid of sodium hyaluronate |
Country Status (1)
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| CN (1) | CN102030837A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110981992A (en) * | 2019-12-27 | 2020-04-10 | 华熙生物科技股份有限公司 | Preparation method of hyaluronic acid for injection |
-
2009
- 2009-09-27 CN CN2009102047270A patent/CN102030837A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110981992A (en) * | 2019-12-27 | 2020-04-10 | 华熙生物科技股份有限公司 | Preparation method of hyaluronic acid for injection |
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Application publication date: 20110427 |