CN102021534A - Method for preparing substrate glass slide with effect of surface enhanced Raman scattering - Google Patents
Method for preparing substrate glass slide with effect of surface enhanced Raman scattering Download PDFInfo
- Publication number
- CN102021534A CN102021534A CN2010105845241A CN201010584524A CN102021534A CN 102021534 A CN102021534 A CN 102021534A CN 2010105845241 A CN2010105845241 A CN 2010105845241A CN 201010584524 A CN201010584524 A CN 201010584524A CN 102021534 A CN102021534 A CN 102021534A
- Authority
- CN
- China
- Prior art keywords
- slide glass
- raman spectrum
- acid
- effect
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a substrate glass slide with the effect of surface enhanced Raman scattering and a preparation method thereof. The prepared metal oxide film substrate glass slide can be stored for a long time, is convenient to use and easy to commercialize, has obvious effect on surface enhanced Raman signal, can better remove fluorescent effect and especially has obvious effect on Raman measurement of organics.
Description
Technical field
The present invention relates to a kind of preparation method of substrate slide glass, particularly relate to the preparation method that a kind of Raman spectrum surface strengthens the substrate slide glass.
Background technology
In recent years, along with the raising of laser apparatus and detector technologies, and Raman can directly measure gas, liquid and solid, and test fast, Raman spectroscopy is applied to daily detection gradually, and deeply is applied in the fields such as archaeology, petrochemical complex, dyestuff, biology, material.But, in organic test, fluorescent effect often appears, Raman signal is covered, have the fluorescent effect of more effectively having eliminated the part organic compound to a certain extent that is created in of laser apparatus now, also can improve Raman signal to a certain extent by strengthening laser radiation intensity with long-time irradiation simultaneously, but there are a lot of organic compound to have fluorescent effect, big bag appears, and long-time and high-intensity laser radiation might cause damage to sample, and these have all limited the application of Raman spectrum in organism to a great extent.
Surface enhanced Raman scattering (SERS) is a kind of unusual surface optical phenomenon, it can amplify the Raman signal of surface molecular about 1,000,000 times, even it is scalable to 100,000,000,000,000 times for some nanoparticle system, as caused electromagnetism strengthens because surperficial local plasmon excimer is excited to be adsorbed on the compound of roughening metallic surface, and the cluster on the uneven surface and the molecule that adsorbs on it constitute Raman enhanced active site, and the two effect makes the Raman scattering of determinand produce great reinforcing effect.But surface enhanced Raman scattering has very big limitation---only have a few metal (gold and silver, copper) can produce so powerful surface enhanced Raman scattering effect, and the substrate of these metals must be the coarse nanoparticle that maybe needs to be prepared into.
The surface enhanced Raman spectroscopy technology has become the focus of Raman signal enhancement techniques in recent years.
Summary of the invention
The present invention proposes the preparation method that a kind of Raman spectrum surface strengthens the substrate slide glass, but the Raman spectrum substrate slide glass prolonged preservation of preparation is easy to use, and is easy to commercialization.
The invention provides the preparation method that a kind of Raman spectrum surface strengthens the substrate slide glass, it is characterized in that, may further comprise the steps:
(1) deposits the metal coating of one deck 400-500nm on slide glass by atomic deposition technique;
(2) with appropriate amount of acid metallic film is carried out mild corrosion;
(3) remove acid remaining on the metallic film with appropriate bases again, washed with de-ionized water makes the Raman spectrum surface and strengthens the substrate slide glass for several times.
Described metal coating is a zinc oxide; Described acid is hydrochloric acid, or nitric acid, or phosphoric acid, and its concentration is 0.0001%-10%; Described alkali comprises sodium hydroxide or potassium hydroxide or ammoniacal liquor, and its concentration is 0.0001%-30%.
The slide glass substrate for preparing can prolonged preservation, is easy to be fixed in microscopically and carries out the Raman spectrum test, and have the effect of significant surface enhanced Raman spectroscopy, and is particularly remarkable to the organism effect.The invention belongs to the technical field of Raman spectrum test.
Description of drawings
The glycine Raman spectrum of Fig. 1 for obtaining on the common slide glass
Fig. 2 is that embodiment 1 makes the glycine Raman spectrum on the Nano zinc oxide film
Embodiment
Below embodiments of the invention are elaborated: present embodiment is being to implement under the prerequisite with the technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
1) preparation of metal oxide coating: with the atomic layer deposition system of Finland picosun company, utilize the presoma zinc methide with deposition of zinc oxide on slide glass, the thick 400nm of zinc oxide coating;
2) mild corrosion of coating: being 0.005% hydrochloric acid with concentration carries out mild corrosion to the slide glass of plated film, soaks 2 minutes;
3) clean: be residual hydrochloric acid in 0.005% sodium hydroxide and on the slide glass after the corrosion with concentration earlier, use washed with de-ionized water again 10 times, make Raman spectrum surface enhancing substrate slide glass.
Glycine is prepared into the solution of saturation concentration, drips on the several slide glasss after corrosion is cleaned, place microscopically to observe slide glass, obtain Raman spectrogram.
Embodiment 2
1) preparation of metal oxide coating: with the atomic layer deposition system of Finland picosun company, utilize the presoma zinc methide with deposition of zinc oxide on slide glass, the thick 500nm of zinc oxide coating;
2) mild corrosion of coating: being 0.001% nitric acid with concentration carries out mild corrosion to the slide glass of plated film, soaks 2 minutes.
3) clean: be residual hydrochloric acid in 0.001% potassium hydroxide and on the slide glass after the corrosion with concentration earlier, use washed with de-ionized water again 10 times, make Raman spectrum surface enhancing substrate slide glass.
Embodiment 3
1) preparation of metal oxide coating: utilize atomic deposition technique, with the atomic layer deposition system of Finland picosun company, utilize the presoma zinc methide with deposition of zinc oxide on slide glass, the thick 400nm of zinc oxide coating;
2) mild corrosion of coating: being 10% phosphoric acid with concentration carries out mild corrosion to the slide glass of plated film, soaks 2 minutes;
3) clean: be residual hydrochloric acid in 30% ammoniacal liquor and on the slide glass after the corrosion with concentration earlier, use washed with de-ionized water again 10 times, make Raman spectrum surface enhancing substrate slide glass.
Claims (3)
1. the Raman spectrum surface strengthens the preparation method of substrate slide glass, it is characterized in that, may further comprise the steps:
(1) deposits the metal coating of one deck 400-500nm on slide glass by atomic deposition technique;
(2) with appropriate amount of acid metallic film is carried out mild corrosion;
(3) remove acid remaining on the metallic film with appropriate bases again, washed with de-ionized water makes the Raman spectrum surface and strengthens the substrate slide glass for several times.
2. strengthen the preparation method of substrate slide glass as claims 1 described Raman spectrum surface, it is characterized in that described metal coating is a zinc oxide.
3. strengthen the preparation method of substrate slide glass as claims 1 described Raman spectrum surface, it is characterized in that described acid is hydrochloric acid, or nitric acid, or phosphoric acid, its concentration is 0.0001%-10%; Described alkali comprises sodium hydroxide or potassium hydroxide or ammoniacal liquor, and its concentration is 0.0001%-30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105845241A CN102021534A (en) | 2010-12-13 | 2010-12-13 | Method for preparing substrate glass slide with effect of surface enhanced Raman scattering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105845241A CN102021534A (en) | 2010-12-13 | 2010-12-13 | Method for preparing substrate glass slide with effect of surface enhanced Raman scattering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102021534A true CN102021534A (en) | 2011-04-20 |
Family
ID=43863303
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105845241A Pending CN102021534A (en) | 2010-12-13 | 2010-12-13 | Method for preparing substrate glass slide with effect of surface enhanced Raman scattering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102021534A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102311095A (en) * | 2011-08-09 | 2012-01-11 | 吉林大学 | Method for preparing multistage metal micro-nanostructures inside micro fluidic chip |
| CN102507531A (en) * | 2011-10-28 | 2012-06-20 | 中国科学院理化技术研究所 | Raman scattering enhanced substrate of oxidation zinc base semiconductor and preparation method and application thereof |
| CN102706843A (en) * | 2012-05-31 | 2012-10-03 | 东南大学 | Method for preparing transparent biological substrate with spectral enhancement function |
| CN102944542A (en) * | 2012-10-18 | 2013-02-27 | 胡建明 | Surface-enhanced Raman substrate of ground glass, and preparation method thereof |
| CN103103494A (en) * | 2013-01-29 | 2013-05-15 | 南京丰强纳米科技有限公司 | Method for preparing oxide surface on surface enhanced raman scattering (SERS) substrate through atomic layer deposition technology |
| CN104280378A (en) * | 2014-09-28 | 2015-01-14 | 李伟 | Method for detecting content of SBS (Styrene Butadiene Styrene) modifier in modified asphalt |
| CN104746049A (en) * | 2015-04-07 | 2015-07-01 | 南京大学 | Method for preparing surface-enhanced Raman scattering base with metal nanometer gaps by utilizing ALD (atomic layer deposition) |
| CN105331943A (en) * | 2015-11-09 | 2016-02-17 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparing method for obtaining Raman enhanced substrate based on sequential co-sputtering and corrosion modification |
| CN106319487A (en) * | 2016-09-09 | 2017-01-11 | 深圳大学 | Manufacture method and manufacture device for plastic surface-enhancing Raman chip |
| CN109540829A (en) * | 2018-12-12 | 2019-03-29 | 暨南大学 | A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole |
| WO2019104438A1 (en) * | 2017-12-01 | 2019-06-06 | Valorisation-Recherche, Limited Partnership | Coated glass slide for enhanced thin tissue section adhesion |
-
2010
- 2010-12-13 CN CN2010105845241A patent/CN102021534A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102311095A (en) * | 2011-08-09 | 2012-01-11 | 吉林大学 | Method for preparing multistage metal micro-nanostructures inside micro fluidic chip |
| CN102311095B (en) * | 2011-08-09 | 2013-11-06 | 吉林大学 | Method for preparing multistage metal micro-nanostructures inside micro fluidic chip |
| CN102507531B (en) * | 2011-10-28 | 2014-03-12 | 中国科学院理化技术研究所 | Raman scattering enhanced substrate of oxidation zinc base semiconductor and preparation method and application thereof |
| CN102507531A (en) * | 2011-10-28 | 2012-06-20 | 中国科学院理化技术研究所 | Raman scattering enhanced substrate of oxidation zinc base semiconductor and preparation method and application thereof |
| CN102706843A (en) * | 2012-05-31 | 2012-10-03 | 东南大学 | Method for preparing transparent biological substrate with spectral enhancement function |
| CN102706843B (en) * | 2012-05-31 | 2014-08-13 | 东南大学 | Method for preparing transparent biological substrate with spectral enhancement function |
| CN102944542A (en) * | 2012-10-18 | 2013-02-27 | 胡建明 | Surface-enhanced Raman substrate of ground glass, and preparation method thereof |
| CN103103494A (en) * | 2013-01-29 | 2013-05-15 | 南京丰强纳米科技有限公司 | Method for preparing oxide surface on surface enhanced raman scattering (SERS) substrate through atomic layer deposition technology |
| CN104280378A (en) * | 2014-09-28 | 2015-01-14 | 李伟 | Method for detecting content of SBS (Styrene Butadiene Styrene) modifier in modified asphalt |
| CN104746049A (en) * | 2015-04-07 | 2015-07-01 | 南京大学 | Method for preparing surface-enhanced Raman scattering base with metal nanometer gaps by utilizing ALD (atomic layer deposition) |
| CN105331943A (en) * | 2015-11-09 | 2016-02-17 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparing method for obtaining Raman enhanced substrate based on sequential co-sputtering and corrosion modification |
| CN106319487A (en) * | 2016-09-09 | 2017-01-11 | 深圳大学 | Manufacture method and manufacture device for plastic surface-enhancing Raman chip |
| WO2019104438A1 (en) * | 2017-12-01 | 2019-06-06 | Valorisation-Recherche, Limited Partnership | Coated glass slide for enhanced thin tissue section adhesion |
| CN109540829A (en) * | 2018-12-12 | 2019-03-29 | 暨南大学 | A kind of infrared spectroscopy detection technique is used for the method for making sample of Crohn disease antidiastole |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102021534A (en) | Method for preparing substrate glass slide with effect of surface enhanced Raman scattering | |
| Wali et al. | Rapid and highly efficient detection of ultra-low concentration of penicillin G by gold nanoparticles/porous silicon SERS active substrate | |
| CN104746049B (en) | The method that the surface enhanced Raman scattering substrate in metal nano gap is prepared using ALD | |
| Viroulaud et al. | Influence of surface pretreatments on the quality of trivalent chromium process coatings on aluminum alloy | |
| Sarfo et al. | Rapid detection of mercury contamination in water by surface enhanced Raman spectroscopy | |
| Zhang et al. | Electrochemiluminescent immunosensor based on CdS quantum dots for ultrasensitive detection of microcystin-LR | |
| Mercier et al. | The role of chelating agents on the corrosion mechanisms of aluminium in alkaline aqueous solutions | |
| CN106093001A (en) | Poly-dopamine silver particles poly-DOPA amine type surface enhanced raman spectroscopy substrate and preparation method thereof | |
| UA109104C2 (en) | Substrate for surface enhanced raman scattering studies | |
| CN102183502A (en) | Method for preparing nano silver film with enhanced Raman scattering substrate based on microwave technique | |
| Stoica et al. | Influence of post-treatment time of trivalent chromium protection coating on aluminium alloy 2024-T3 on improved corrosion resistance | |
| Fryling et al. | Resonance Raman observation of surface carbonyl groups on carbon electrodes following dinitrophenylhydrazine derivatization | |
| Proniewicz et al. | SERS activity and spectroscopic properties of Zn and ZnO nanostructures obtained by electrochemical and green chemistry methods for applications in biology and medicine | |
| Cho et al. | Surface-plasmon-induced azo coupling reaction between nitro compounds on dendritic silver monitored by surface-enhanced Raman spectroscopy | |
| CN108333166A (en) | The surface enhanced Raman scattering substrate and preparation method of induced with laser | |
| Patty et al. | Enhancement of emission efficiency of colloidal CdSe quantum dots on silicon substrate via an ultra-thin layer of aluminum oxide | |
| Juine et al. | Surfactant-free green synthesis of ZnS QDs with active surface defects for selective nanomolar oxalic acid colorimetric sensors at room temperature | |
| CN102944542A (en) | Surface-enhanced Raman substrate of ground glass, and preparation method thereof | |
| Osorio-Román et al. | Surface-enhanced spectra on D-gluconic acid coated silver nanoparticles | |
| CN102706843B (en) | Method for preparing transparent biological substrate with spectral enhancement function | |
| Lee et al. | Silver-coated nanoporous gold skeletons for fluorescence amplification | |
| Shang et al. | Micro-hole array sprayer combined with an organic membrane to assist LIBS (MASOM-LIBS): A novel highly sensitive detection method for dissolved trace heavy metals in water | |
| CN104316508A (en) | Method for preparing SERS active substrate with silver nanoparticles in linear distribution | |
| CN113433110B (en) | Preparation method for generating substrate with honeysuckle dendritic crystal flower-like nano structure by in-situ substitution method | |
| Chen et al. | Preparation of silver nanoparticles on zinc oxide nanowires by photocatalytic reduction for use in surface‐enhanced Raman scattering measurements |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110420 |