CN102021229A - Method for detecting cations in aqueous solution - Google Patents
Method for detecting cations in aqueous solution Download PDFInfo
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- CN102021229A CN102021229A CN2009101524408A CN200910152440A CN102021229A CN 102021229 A CN102021229 A CN 102021229A CN 2009101524408 A CN2009101524408 A CN 2009101524408A CN 200910152440 A CN200910152440 A CN 200910152440A CN 102021229 A CN102021229 A CN 102021229A
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Abstract
The invention relates to a method for detecting cations in aqueous solution. On the basis of a nano-nucleic acid copolymer system, by using mutual complexation of nucleic acid and the cations, the melting temperature of the system rises, so that the color change of the solution is determined by naked eyes or simple instruments and equipment according to the lag characteristic of the color change of the solution and toxic cations in the aqueous solution system can be rapidly detected.
Description
Technical field
The present invention relates to the ion detection method, relate in particular to cationic detection method in a kind of aqueous solution.
Background technology
Water and human existence, health and develop closely bound uply, water environment pollution and shortage of water resources are the two large problems that global Freshwater resources are faced with.The water resources ownership per capita of China only is 1/4 of a world average level, along with China's rapid economic development, the increase of population, the progressively raising of living standards of the people, the quickening of industrialization and urbanization paces, water consumption sharply increases, and the also corresponding increase of quantity of wastewater effluent has aggravated the shortage of Freshwater resources and the pollution of water surrounding.Therefore, protection also rationally utilizes limited water resource, reduces and the improvement water environment pollution, has become to concern one of human survival, development and problem demanding prompt solution.
Many toxicity positively charged ions such as Hg
2+, Pb
2+, Cd
2+, Cu
2+, Co
2+, Cr
3+, Ni
2+, Pd
2+, Mn
2+, Mn
3+And Ag
+ etc.Extensively be present in the natural water resources system.Along with steady development of economy and human continuous increase to energy demand, environmental pollution has become a very important problem.Industry " three wastes " (waste water,waste gas and industrial residue) has caused the discharging of very havoc, particularly trade effluent especially outstanding to the pollution of water resources to environment.Contain a large amount of heavy metal ion in the trade effluent of industries such as industrial and mineral, chemical industry, battery, plating, indivedual enterprises do not add these waste water to handle or handle a little and just are discharged in the water body, cause heavy metal ion enrichment in water body, accumulation, cause water pollution.The sewage that contains heavy metal ion is directly drunk by the mankind, perhaps is used to carry out aquaculture and crop irrigation etc., directly or indirectly influences human existence and health.
For instance, Hg
2+Be the most stable existence form of inorganic mercury, brain, neural system, kidney and the endocrine system etc. to the human or animal produce very serious destruction easily; In addition, rivers and lakes and marine microbe can convert the inorganic mercury ion to methyl mercury (CH
3Hg
+) accumulate in vivo, enter in people or other animal body by food chain, directly threaten human beings'health.In the contaminated tap water if Pb
2+Too high levels, the cerebral tissue and the nerve conduction system of meeting harmful to human become the potential carcinogens, especially influence children's intelligence and body development; Because Pb
2+To the long-term hazards that human health may constitute, the World Health Organization is with Pb in the tap water
2+The upper content limit standard setting is 10 μ g/L.In addition, other toxicity positively charged ion is accumulated to finite concentration in human body, can cause the damage of human organ and tissue equally, causes that human body is poisoned or even death, the serious threat mankind's life security.
At present, mercury ion or the cationic method of other toxicity mainly comprise in the detection water solution system: 1) based on detecting fluorescence chromophore, the method for sensing of the bacterium of genetic engineering modification and conjugated polymer and golden nanometer particle; 2) electroanalysis chemical process is as capillary electrophoresis, ion specific electrode method or cyclic voltammetry; 3) inductively coupled plasma mass spectrometry technology used in conjunction (ICP-MS) etc.Above method is when detecting water solution system toxic positively charged ion, and sample preparation steps complexity, sample poorly water-soluble, ion interference are serious, and needs the plant and instrument of large-scale costliness usually.These restrictions are very unfavorable to the cationic rapid detection of water solution system toxic, because a lot of condition does not possess or the jejune unit of technology or individuality can't detect water quality, cause source of pollution to enlarge; And, utilize these methods can not realize detecting in real time, on the spot to water quality.
In order to reduce environmental pollution, guarantee human health existence and the Sustainable development that keeps economic society, accomplish early to detect, early administer, reduce the cost of water pollution control, water pollution is controlled at minimum level to the mankind's harm, must accomplishes to detect fast, in real time the Hg in the water body
2+, Pb
2+, Cu
2+, Ni
2+, Mn
2+/ Mn
3+, Ag
+Etc. the toxicity positively charged ion.Therefore, develop a kind of highly sensitive, selectivity good, the cationic method for quick of water solution system toxic simple to operate and that cost is low seems particularly urgent and important.
Summary of the invention
How fast, accurately and simply technical problem to be solved by this invention is: detect the toxicity positively charged ion in the water solution system.
For addressing the above problem, the invention provides cationic detection method in the aqueous solution, comprise step: provide two parts of identical containing to be coupled with the nanometer gold of nucleic acid molecule or the detection solution of nanometer silver; Isopyknic described cationic water and detected aqueous solution of not containing is added described two parts respectively and detects in the solution, form corresponding first mixing solutions and second mixing solutions; Respectively described first mixing solutions and second mixing solutions are heated up; Judge whether there is the positively charged ion that is detected in the detected aqueous solution according to the colour-change of described first mixing solutions and described second mixing solutions.
The method of judging is specially: if described first mixing solutions and the variable color under differing temps of second mixing solutions are then judged and contained tested positively charged ion in the described aqueous solution; If described first mixing solutions and the variable color under uniform temp of second mixing solutions are then judged and are not contained tested positively charged ion in the described aqueous solution.
If tested positively charged ion is Pb
2+, then the sequence of described nucleic acid molecule is:
5 '-CATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT-3 ' and
5’-ACTCACTATrAGGAAGAGATG-3’，
RA in the described sequence of nucleic acid molecules is an adenosine Yeast Nucleic Acid.
Judge in the described detected aqueous solution and whether contain Pb
2+The ionic method is specially: if described first mixing solutions 36 ℃ of following variable colors and second mixing solutions is being higher than 36 ℃ of following variable colors, is then judged in the described aqueous solution to contain Pb
2+Ion; If 36 ℃ of following variable colors, then judging, described first mixing solutions and second mixing solutions do not contain Pb in the described aqueous solution
2+Ion.
If tested positively charged ion is Cu
2+, then the sequence of described nucleic acid molecule is:
5 '-CACATTASTGTTGTA-3 ' and 3 '-GTGTAATSACAACAT-5 ',
S in the described sequence of nucleic acid molecules is a salicylaldhyde.
Judge in the described detected aqueous solution and whether contain Cu
2+The ionic method is specially: if described first mixing solutions 36 ℃ of following variable colors and described second mixing solutions is being higher than 36 ℃ of following variable colors, is then judged in the described aqueous solution to contain Cu
2+Ion; If 36 ℃ of following variable colors, then judging, described first mixing solutions and second mixing solutions do not contain Cu in the described aqueous solution
2+Ion.
If tested positively charged ion is Ni
2+, then the sequence of described nucleic acid molecule is:
5 '-CTTTCTPur
PTCCCT-3 ' and 5 '-AGGGAPur
PAGAAAG-3 ',
Pur in the described sequence of nucleic acid molecules
PBe six pyridyl purine.
Judge in the described detected aqueous solution and whether contain Ni
2+The ionic method is specially: if described first mixing solutions 29 ℃ of following variable colors and described second mixing solutions is being higher than 29 ℃ of following variable colors, is then judged in the described aqueous solution to contain Ni
2+Ion; If 29 ℃ of following variable colors, then judging, described first mixing solutions and second mixing solutions do not contain Ni in the described aqueous solution
2+Ion.
If tested positively charged ion is Mn
2+Or Mn
3+, then the sequence of described nucleic acid molecule is:
5 '-CGGCCSSSSSSSSSSCGCGC-3 ' and
3’-GCCGGSSSSSSSSSSGCGCG-5’，
S in the described sequence of nucleic acid molecules is a salicylaldhyde.
Judge in the described detected aqueous solution and whether contain Mn
2+Or Mn
3+The ionic method is specially: if described first mixing solutions 28 ℃ of following variable colors and described second mixing solutions is being higher than 28 ℃ of following variable colors, is then judged in the described aqueous solution to contain Mn
2+Or Mn
3+Ion; If 28 ℃ of following variable colors, then judging, described first mixing solutions and second mixing solutions do not contain Mn in the described aqueous solution
2+Or Mn
3+Ion.
If tested positively charged ion is Ag
+, then the sequence of described nucleic acid molecule is:
5 '-CACSTTASTGTSGTA-3 ' and 3 '-GTGSAATSACASCAT-5 ',
S in the described sequence of nucleic acid molecules is a salicylaldhyde.
Judge in the described detected aqueous solution and whether contain Ag
+The ionic method is specially: if described first mixing solutions 23 ℃ of following variable colors and described second mixing solutions is being higher than 23 ℃ of following variable colors, is then judged in the described aqueous solution to contain Ag
+Ion; If 23 ℃ of following variable colors, then judging, described first mixing solutions and second mixing solutions do not contain Ag in the described aqueous solution
+Ion.
Be coupling at nucleic acid molecule under the situation of nanometer gold, described variable color is for becoming bright red; And be coupling at nucleic acid molecule under the situation of nanometer silver, described variable color is orange-yellow for becoming.
The present invention is based on nanometer-nucleic acid copolymer systems, utilize the mutual complexing action of nucleic acid and positively charged ion and cause the system melt temperature to raise, make solution colour change the characteristic that lags behind, variation by naked eyes or simple plant and instrument differentiation solution colour gets final product the toxicity positively charged ion in the rapid detection water solution system.The method that the present invention asks for protection is simple to operate, the detection cost is low, detection sensitivity is high.Be suitable in the sewage effluent water quality detection, water quality detection after the sewage disposal, domestic water/process water detects, the urgent water safety of outdoors detects, agriculture and animal husbandry field people and animals' tap water detects, culture the fishery water quality detection, the intake water quality detection, lake water quality of river investigation, pollute and distribute, source of pollution are followed the trail of, solution positively charged ion in the scientific experiment detects, field irrigation, the water quality detection of flower planting water, liquid-food/beverage/pure water detection and common plunge bath/natatorium water quality detection etc.As long as the cationic concentration of water solution system toxic is 1 * 10
-6More than the mol/L, utilize this method all can realize convenience, sensitive and detection fast.
Embodiment
In the specific embodiment of the present invention, comprise and detect formulations prepared from solutions embodiment and test implementation example.Wherein detect preparation process and parameter that formulations prepared from solutions embodiment provides a plurality of detection solution, and the test implementation example provides process and parameter that the detection solution of a plurality of application preparations detects the positively charged ion in the aqueous solution.
The preparation that detects solution comprises following step:
(1) preparation of golden nanometer particle or Nano silver grain;
(2) comprise the nucleic acid molecule of particular sequence according to detected cation selective;
(3) utilize sulfydryl to realize the coupling of particular sequence nucleic acid molecule and golden nanometer particle or Nano silver grain;
(4) preparation of detection solution.
In the above-mentioned steps, the preparation method of golden nanometer particle or Nano silver grain is well known to those skilled in the art, does not repeat them here.
The nucleic acid molecule that comprises particular sequence among the present invention can be from the purchases such as TriLink BioTechnologies company of the Link Technologies company or the U.S. of Britain.
Below detect formulations prepared from solutions embodiment and will realize that the coupling of particular sequence nucleic acid molecule and golden nanometer particle or Nano silver grain and the process for preparation of detection solution are described in detail utilizing sulfydryl.
Detect formulations prepared from solutions embodiment 1
(1) with 11.468g Na
2HPO
4With 0.509g NaH
2PO
4Be dissolved in the 500mL ultrapure water, prepare the disulfide linkage lysis buffer, and be dithiothreitol (DTT) (DTT) the solution 1mL of solvent preparation 0.1mol/L with the damping fluid.
(2) get the solution 100 μ L that prepare in the step (1) and join in the freeze dried nucleic acid molecule of 5nmol, nucleotide sequence is:
5 '-CATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT-3 ' and
5’-ACTCACTATrAGGAAGAGATG-3’，
Wherein rA is an adenosine Yeast Nucleic Acid;
With the foil paper parcel and after at room temperature leaving standstill 3h, carry out repeatedly centrifugal and the freeze-drying processing to it.
(3) getting the nucleic acid molecule 4hmol that modifies in the step (2), to join 1mL concentration be that the diameter of 17nmol/L is in the solution of gold nanoparticles of 12nm, with the foil paper parcel and at room temperature stir 12h; Then an amount of phosphoric acid salt being regulated damping fluid, for example can be 0.562g Na
2HPO
4And 0.125gNaH
2PO
4The damping fluid that is dissolved in the 50mL ultrapure water joins in the solution of gold nanoparticles, and making phosphatic concentration is 9mmol/L, also at room temperature stirs 30min with the foil paper parcel; At last that solution is centrifugal repeatedly and be dispersed in 200 μ L lavation buffer solutions, for example can be in the damping fluid that mixes of the NaCl solution of the phosphate buffered saline buffer of 10mmol/L and 150mmol/L.
(4) get the particular sequence nucleic acid gold nano-particles modified solution 100 μ L that prepare in the step (3), centrifugal and be dispersed in 500 μ L and detect damping fluid, for example can be in the NaCl solution and damping fluid that 0.1% sodium laurylsulfonate (SDS) mixes of phosphate solution, 150mmol/L of 10mmol/L, centrifugal repeatedly several; It is dispersed in an amount of detection damping fluid at last, the concentration that makes particular sequence nucleic acid gold nano-particles modified is 1nmol/L, stores stand-by.
Detect formulations prepared from solutions embodiment 2
All the other steps are identical with embodiment 1, only the golden nanometer particle among the embodiment 1 become Nano silver grain.
Detect formulations prepared from solutions embodiment 3
All the other steps are identical with embodiment 1, only the nucleotide sequence among the embodiment 1 become:
5 '-CACATTASTGTTGTA-3 ' and 3 '-GTGTAATSACAACAT-5 ',
Wherein S is a salicylaldhyde.
Detect formulations prepared from solutions embodiment 4
All the other steps are identical with embodiment 3, only the golden nanometer particle among the embodiment 3 become Nano silver grain.
Detect formulations prepared from solutions embodiment 5
All the other steps are identical with embodiment 1, only the nucleotide sequence among the embodiment 1 become:
5 '-CTTTCTPur
PTCCCT-3 ' and 5 '-AGGGAPur
PAGAAAG-3 ',
Pur wherein
PBe six pyridyl purine.
Detect formulations prepared from solutions embodiment 6
All the other steps are identical with embodiment 5, only the golden nanometer particle among the embodiment 5 become Nano silver grain.
Detect formulations prepared from solutions embodiment 7
All the other steps are identical with embodiment 1, only the nucleotide sequence among the embodiment 1 become:
5 '-CGGCCSSSSSSSSSSCGCGC-3 ' and
3’-GCCGGSSSSSSSSSSGCGCG-5’，
Wherein S is a salicylaldhyde.
Detect formulations prepared from solutions embodiment 8
All the other steps are identical with embodiment 7, only the golden nanometer particle among the embodiment 7 become Nano silver grain.
Detect formulations prepared from solutions embodiment 9
All the other steps are identical with embodiment 1, only the nucleotide sequence among the embodiment 1 become:
5 '-CACSTTASTGTSGTA-3 ' and 3 '-GTGSAATSACASCAT-5 ',
Wherein S is a salicylaldhyde.
Detect formulations prepared from solutions embodiment 10
All the other steps are identical with embodiment 9, only the golden nanometer particle among the embodiment 9 become Nano silver grain.
Detect embodiment 1
At first, prepare the test tube A and the B of two same specifications;
Then, the nucleic acid gold nano-particles modified that comprises following sequence and the phosphate solution of 10mmol/L, the NaCl solution of 150mmol/L and 0.1% the SDS that adds the aforementioned preparation of identical and equivalent in two test tubes respectively forms detection solution:
5 '-CATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT-3 ' and
5’-ACTCACTATrAGGAAGAGATG-3’
Wherein rA is an adenosine Yeast Nucleic Acid; Then, in test tube A and B, add isopyknic ultrapure water and water sample to be detected respectively;
At last, test tube A and B are placed in the water-bath device, the temperature rise rate of regulating the water-bath device is 1 ℃/min;
When bath temperature is elevated to 36 ℃,, then judge to detect in the water sample not cation Pb if the color of solution becomes bright red simultaneously among test tube A and the B
2+
If the color of solution becomes bright red among the test tube A in the time of 36 ℃, and the color of solution just becomes bright red among the test tube B when about 50 ℃ of left and right sides, then judges to detect in the water sample to contain positively charged ion Pb
2+
Detect embodiment 2
Other steps are identical with embodiment 1, only will be changed to the Nano silver grain of being modified by the nucleic acid particular sequence by particular sequence nucleic acid gold nano-particles modified among the embodiment 1;
When bath temperature is elevated to 36 ℃,, then judge to detect in the water sample not cation Pb if the color of solution becomes orange-yellowly simultaneously among test tube A and the B
2+
If the color of solution becomes orange-yellowly in the time of 36 ℃ among the test tube A, and the color of solution just becomes orange-yellowly when about 50 ℃ of left and right sides among the test tube B, then judges to detect in the water sample and contains positively charged ion Pb
2+
Detect embodiment 3
All the other steps are identical with embodiment 1, just nucleotide sequence become following sequence: 5 '-CACATTASTGTTGTA-3 ' and 3 '-GTGTAATSACAACAT-5 ',
Wherein S is a salicylaldhyde.When bath temperature is elevated to 36 ℃,, then judge to detect in the water sample not cation Cu if the color of solution becomes bright red simultaneously among test tube A and the B
2+
If the color of solution becomes bright red among the test tube A in the time of 36 ℃, and the color of solution just becomes bright red among the test tube B when about 71 ℃ of left and right sides, then judges to detect in the water sample to contain positively charged ion Cu
2+
Detect embodiment 4
Other steps are identical with embodiment 3, only will be changed to the Nano silver grain of being modified by the nucleic acid particular sequence by particular sequence nucleic acid gold nano-particles modified among the embodiment 3;
When bath temperature is elevated to 36 ℃,, then judge to detect in the water sample not cation Cu if the color of solution becomes orange-yellowly simultaneously among test tube A and the B
2+
If the color of solution becomes orange-yellowly in the time of 36 ℃ among the test tube A, and the color of solution just becomes orange-yellowly when about 71 ℃ of left and right sides among the test tube B, then judges to detect in the water sample and contains positively charged ion Cu
2+
Detect embodiment 5
All the other steps are identical with embodiment 1, just nucleotide sequence become following sequence:
5 '-CTTTCTPur
PTCCCT-3 ' and 5 '-AGGGAPur
PAGAAAG-3 ',
Pur wherein
PBe six pyridyl purine.
When bath temperature is elevated to 29 ℃,, then judge to detect in the water sample not cation Ni if the color of solution becomes bright red simultaneously among test tube A and the B
2+
If the color of solution becomes bright red among the test tube A in the time of 29 ℃, and the color of solution just becomes bright red among the test tube B when about 46 ℃ of left and right sides, then judges to detect in the water sample to contain positively charged ion Ni
2+
Detect embodiment 6
Other steps are identical with embodiment 5, only will be changed to the Nano silver grain of being modified by particular sequence nucleic acid by particular sequence nucleic acid gold nano-particles modified among the embodiment 5;
When bath temperature is elevated to 29 ℃,, then judge to detect in the water sample not cation Ni if the color of solution becomes orange-yellowly simultaneously among test tube A and the B
2+
If the color of solution becomes orange-yellowly in the time of 29 ℃ among the test tube A, and the color of solution just becomes orange-yellowly when about 46 ℃ of left and right sides among the test tube B, then judges to detect in the water sample and contains positively charged ion Ni
2+
Detect embodiment 7
All the other steps are identical with embodiment 1, just nucleotide sequence become following sequence:
5 '-CGGCCSSSSSSSSSSCGCGC-3 ' and
3’-GCCGGSSSSSSSSSSGCGCG-5’，
Wherein S is a salicylaldhyde.
When bath temperature is elevated to 28 ℃,, then judge to detect in the water sample not cation Mn if the color of solution becomes bright red simultaneously among test tube A and the B
2+Or Mn
3+
If the color of solution becomes bright red among the test tube A in the time of 28 ℃, and the color of solution just becomes bright red among the test tube B when about 69 ℃ of left and right sides, then judges to detect in the water sample to contain positively charged ion Mn
2+Or Mn
3+
Detect embodiment 8
Other steps are identical with embodiment 7, only will be changed to the Nano silver grain of being modified by particular sequence nucleic acid by particular sequence nucleic acid gold nano-particles modified among the embodiment 7;
When bath temperature is elevated to 28 ℃,, then judge to detect in the water sample not cation Mn if the color of solution becomes orange-yellowly simultaneously among test tube A and the B
2+Or Mn
3+
If the color of solution becomes orange-yellowly in the time of 28 ℃ among the test tube A, and the color of solution just becomes orange-yellowly when about 69 ℃ of left and right sides among the test tube B, then judges to detect in the water sample and contains positively charged ion Mn
2+Or Mn
3+
Detect embodiment 9
All the other steps are identical with embodiment 1, just nucleotide sequence become following sequence:
5 '-CACSTTASTGTSGTA-3 ' and 3 '-GTGSAATSACASCAT-5 ',
Wherein S is a salicylaldhyde.
When bath temperature is elevated to 23 ℃,, then judge to detect in the water sample not cation Ag if the color of solution becomes bright red simultaneously among test tube A and the B
+
If the color of solution becomes bright red among the test tube A in the time of 23 ℃, and the color of solution just becomes bright red among the test tube B when about 45 ℃ of left and right sides, then judges to detect in the water sample to contain cation A g
+
Detect embodiment 10
Other steps are identical with embodiment 9, only will be changed to the Nano silver grain of being modified by particular sequence nucleic acid by particular sequence nucleic acid gold nano-particles modified among the embodiment 9;
When bath temperature is elevated to 23 ℃,, then judge to detect in the water sample not cation Ag if the color of solution becomes orange-yellowly simultaneously among test tube A and the B
+
If the color of solution becomes orange-yellowly in the time of 23 ℃ among the test tube A, and the color of solution just becomes orange-yellowly when about 45 ℃ of left and right sides among the test tube B, then judges to detect in the water sample and contains cation A g
+
Though the present invention with preferred embodiment openly as above; but it is not to be used for limiting claim; any those skilled in the art without departing from the spirit and scope of the present invention; can make possible change and modification, so protection scope of the present invention should be as the criterion with the scope that claim of the present invention was defined.
Claims (8)
1. cationic detection method in the aqueous solution is characterized in that, comprises step:
The nanometer gold that containing of two parts of identical and equivalent be coupled with nucleic acid molecule or the detection solution of nanometer silver are provided;
Isopyknic described cationic water and detected aqueous solution of not containing is added described two parts respectively and detects in the solution, form corresponding first mixing solutions and second mixing solutions;
Respectively described first mixing solutions and second mixing solutions are heated up;
If described first mixing solutions and the variable color under differing temps of second mixing solutions are then judged and are contained tested positively charged ion in the described aqueous solution;
If described first mixing solutions and the variable color under uniform temp of second mixing solutions are then judged and are not contained tested positively charged ion in the described aqueous solution.
2. cationic detection method in the aqueous solution as claimed in claim 1 is characterized in that: tested positively charged ion is Pb
2+, the sequence of described nucleic acid molecule is:
5 '-CATCTCTTCTCCGAGCCGGTCGAAATAGTGAGT-3 ' and
5’-ACTCACTATrAGGAAGAGATG-3’，
RA in the described sequence of nucleic acid molecules is an adenosine Yeast Nucleic Acid.
3. cationic detection method in the aqueous solution as claimed in claim 1 is characterized in that: tested positively charged ion is Cu
2+, the sequence of described nucleic acid molecule is:
5 '-CACATTASTGTTGTA-3 ' and
3’-GTGTAATSACAACAT-5’，
S in the described sequence of nucleic acid molecules is a salicylaldhyde.
4. cationic detection method in the aqueous solution as claimed in claim 1 is characterized in that: tested positively charged ion is Ni
2+, the sequence of described nucleic acid molecule is:
5 '-CTTTCTPur
PTCCCT-3 ' and
5’-AGGGAPur
PAGAAAG-3’，
Pur in the described sequence of nucleic acid molecules
PBe six pyridyl purine.
5. cationic detection method in the aqueous solution as claimed in claim 1 is characterized in that: tested positively charged ion is Mn
2+Or Mn
3+, the sequence of described nucleic acid molecule is:
5 '-CGGCCSSSSSSSSSSCGCGC-3 ' and
3’-GCCGGSSSSSSSSSSGCGCG-5’，
S in the described sequence of nucleic acid molecules is a salicylaldhyde.
6. cationic detection method in the aqueous solution as claimed in claim 1 is characterized in that: tested positively charged ion is Ag
+, the sequence of described nucleic acid molecule is:
5 '-CACSTTASTGTSGTA-3 ' and 3 '-GTGSAATSACASCAT-5 ',
S in the described sequence of nucleic acid molecules is a salicylaldhyde.
7. as cationic detection method in each described aqueous solution in the claim 1 to 6, it is characterized in that: described nucleic acid molecule is coupled in nanometer gold, and described variable color is for becoming bright red.
8. as cationic detection method in each described aqueous solution in the claim 1 to 6, it is characterized in that: described nucleic acid molecule is coupled in nanometer silver, and described variable color is orange-yellow for becoming.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101524408A CN102021229A (en) | 2009-09-14 | 2009-09-14 | Method for detecting cations in aqueous solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101524408A CN102021229A (en) | 2009-09-14 | 2009-09-14 | Method for detecting cations in aqueous solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102021229A true CN102021229A (en) | 2011-04-20 |
Family
ID=43863007
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|---|---|---|---|
| CN2009101524408A Pending CN102021229A (en) | 2009-09-14 | 2009-09-14 | Method for detecting cations in aqueous solution |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621091A (en) * | 2012-04-01 | 2012-08-01 | 中国科学院宁波材料技术与工程研究所 | Method for quickly detecting copper ions in solution |
| CN107764813A (en) * | 2017-09-01 | 2018-03-06 | 杨蕾 | A kind of method detected based on aptamers DNAzyme to lead ion |
| CN108982458A (en) * | 2018-08-14 | 2018-12-11 | 江苏科技大学 | A kind of magnetic bead particles based on deoxyribozyme modification are used for the fluorescent method of zinc ion detection |
| CN109142341A (en) * | 2018-08-13 | 2019-01-04 | 南华大学 | A method of detection underwater trace metal ion |
-
2009
- 2009-09-14 CN CN2009101524408A patent/CN102021229A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621091A (en) * | 2012-04-01 | 2012-08-01 | 中国科学院宁波材料技术与工程研究所 | Method for quickly detecting copper ions in solution |
| CN102621091B (en) * | 2012-04-01 | 2014-07-30 | 中国科学院宁波材料技术与工程研究所 | Method for quickly detecting copper ions in solution |
| CN107764813A (en) * | 2017-09-01 | 2018-03-06 | 杨蕾 | A kind of method detected based on aptamers DNAzyme to lead ion |
| CN109142341A (en) * | 2018-08-13 | 2019-01-04 | 南华大学 | A method of detection underwater trace metal ion |
| CN109142341B (en) * | 2018-08-13 | 2022-06-03 | 南华大学 | Method for detecting trace metal ions in water |
| CN108982458A (en) * | 2018-08-14 | 2018-12-11 | 江苏科技大学 | A kind of magnetic bead particles based on deoxyribozyme modification are used for the fluorescent method of zinc ion detection |
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