CN102009964B - Method for extracting and detecting nitrate and nitrite in Chinese sauerkraut - Google Patents
Method for extracting and detecting nitrate and nitrite in Chinese sauerkraut Download PDFInfo
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- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 37
- 235000021108 sauerkraut Nutrition 0.000 title claims abstract description 33
- 229910002651 NO3 Inorganic materials 0.000 title abstract description 11
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 title abstract description 11
- 238000001514 detection method Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 150000002823 nitrates Chemical class 0.000 claims description 30
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 claims description 16
- YKYOUMDCQGMQQO-UHFFFAOYSA-L cadmium dichloride Chemical compound Cl[Cd]Cl YKYOUMDCQGMQQO-UHFFFAOYSA-L 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 13
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000012488 sample solution Substances 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 8
- XWNSFEAWWGGSKJ-UHFFFAOYSA-N 4-acetyl-4-methylheptanedinitrile Chemical compound N#CCCC(C)(C(=O)C)CCC#N XWNSFEAWWGGSKJ-UHFFFAOYSA-N 0.000 claims description 7
- 239000004153 Potassium bromate Substances 0.000 claims description 7
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 7
- 229940094037 potassium bromate Drugs 0.000 claims description 7
- 235000019396 potassium bromate Nutrition 0.000 claims description 7
- 229910021529 ammonia Inorganic materials 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 5
- 230000010355 oscillation Effects 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 abstract description 14
- 230000008569 process Effects 0.000 abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 2
- CEKJAYFBQARQNG-UHFFFAOYSA-N cadmium zinc Chemical compound [Zn].[Cd] CEKJAYFBQARQNG-UHFFFAOYSA-N 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 35
- 230000009467 reduction Effects 0.000 description 8
- 235000013311 vegetables Nutrition 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 229910021538 borax Inorganic materials 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012047 saturated solution Substances 0.000 description 3
- 239000004328 sodium tetraborate Substances 0.000 description 3
- 235000010339 sodium tetraborate Nutrition 0.000 description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- DBJUEJCZPKMDPA-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O DBJUEJCZPKMDPA-UHFFFAOYSA-N 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- -1 nitrite forms nitrosamines Chemical class 0.000 description 2
- 238000005554 pickling Methods 0.000 description 2
- 239000000276 potassium ferrocyanide Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 206010011703 Cyanosis Diseases 0.000 description 1
- 206010012373 Depressed level of consciousness Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000003969 polarography Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 description 1
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical class [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
检测酸菜中硝酸盐和亚硝酸盐的方法，涉及一种提取的方法。本发明解决了现有提取酸菜中硝酸盐和亚硝酸盐方法存在时间长、效率低操作复杂问题，及检测方法存在工艺复杂、耗时长、线性范围窄等缺点。本发明利用微波法辅助提取酸菜中的硝酸盐、亚硝酸盐。利用锌-镉水浴振荡法还原硝酸盐，采用光化学方法检测硝酸盐和亚硝酸盐。本发明所述提取方法具有工艺简单、耗时短、提取率高、设备简单、适用范围广、重现性好、节省时间、节省试剂、污染小等特点。本发明所述检测方法具有工艺简单、耗时短、灵敏度高、线性范围宽、仪器设备简单的优点。A method for detecting nitrate and nitrite in sauerkraut relates to an extraction method. The invention solves the problems of long time, low efficiency and complex operation in the existing method for extracting nitrate and nitrite in sauerkraut, and the shortcomings of the detection method such as complex process, long time consumption and narrow linear range. The invention utilizes a microwave method to assist in extracting nitrate and nitrite in sauerkraut. Nitrate was reduced by zinc-cadmium water bath shaking method, and nitrate and nitrite were detected by photochemical method. The extraction method of the invention has the characteristics of simple process, short time consumption, high extraction rate, simple equipment, wide application range, good reproducibility, time saving, reagent saving, little pollution and the like. The detection method of the invention has the advantages of simple process, short time consumption, high sensitivity, wide linear range and simple equipment.
Description
Technical field
The present invention relates to a kind of method of detection.
Background technology
Sauerkraut is raw material as the common salted vegetables in a kind of north with Chinese cabbage, adopts the method storage of pickling, with this shortage of replenishing winter vegetable.In curing process, ferment by natural certain micro-organisms attached to vegetable surface, under the effect of reduction bacterium, the nitrate salt in the vegetables can be reduced into nitrite.There are some researches show
[1], vegetables are in the process of pickling, and the content of its nitrite presents the back downward trend that rises earlier.The sauerkraut that exceeds standard when the edible nitrite content of people, nitrite poisoning together, the lighter's giddy, palpitaition, vomiting, cyanotic lips, are twitched, are short of breath at weight person obnubilation, but the untimely threat to life of rescue.In addition, nitrite forms nitrosamines with the effect of secondary amine class inside and outside human body
[2], it is carcinogenic, teratogenesis, mutagenic material when in human body, reaching doses, but the serious harm HUMAN HEALTH.
About nitrate salt, the extraction of nitrite, traditional extraction process has solvent-extraction process
[3], hot water extraction method, vibration extraction method
[4]Deng, but they have the some shortcomings part in leaching process, for example, extraction time long (15～20min), extraction yield low (75%～85%), complicated operation etc.
At present, the method for directly measuring nitrate salt is less, mainly is it to be reduced into nitrite measure.Reduction nitrate salt majority is to adopt the cadmium post to reduce, though this method reduction efficiency height, system cadmium grain, the dress post, reduction, washing, mensuration and activation step are loaded down with trivial details, length consuming time.
Have a variety ofly about the nitrite detection method, the method that detects nitrite at present mainly contains the griess reagent colourimetry, the catalysis light-intensity method, and oscilloscopic polarography etc., these method sensitivity are low, and interference is many, is subject to shortcomings such as reagent color influence.The method for quick of nitrite such as test kit, rapid detection appearance, shortcoming such as it is narrow to have linearity range again, and detection limit is low.
Summary of the invention
The objective of the invention is in order to solve existing nitrate salt, the efficient low complicated operation problem long in the sauerkraut of extracting, reach detection method and have shortcomings such as complex process, length consuming time, linearity range be narrow with nitrite method lifetime.
The method of nitrate salt and nitrite of extracting among the present invention in the sauerkraut is undertaken by following step: sauerkraut is rubbed, get 1g then and join 2.5mL borax saturated solution and stir adding 10mL zero(ppm) water through rubbing sauerkraut after handling; Then at extracting through microwave heating 5min; The temperature of microwave heating is 80 ℃, and adding potassium ferrocyanide solution and the 1mL concentration that 1mL concentration is 0.25mol/L again is the acetic acid zinc solution of 1mol/L, constant volume; Static filtration is collected filtrating and is obtained extracting solution; Promptly accomplish nitrate salt and nitrite extraction in the sauerkraut.
The said extracted method is under the microwave field effect; Separated from matrix or system with nitrite by nitrate salt through the difference that absorbs the microwave ability is feasible; Enter into that specific inductivity is less, the borax saturated solution of microwave absorption capacity relative mistake; Thereby accomplished the extraction of nitrate salt and nitrite, it has, and simple, the consuming time weak point of technology, extraction yield are high, equipment is simple, applied widely, favorable reproducibility, save time, save reagent, pollute characteristics such as little.The extraction time of the inventive method is 5min, and nitrate salt and nitrite extraction yield are all more than 90%.
Detecting in the sauerkraut nitrate salt among the present invention accomplishes by following step with nitrite: one, the preparation of o-aminophthalylhydrazide analytic liquid: absorption 7.5mL concentration is 1 * 10
-3The mol/L luminol solution uses concentration to obtain the o-aminophthalylhydrazide analytic liquid as the NaOH solution constant volume of 0.25mol/L; Two, the mensuration of nitrite: get the extracting solution that 5.00mL extracts from sauerkraut as stated above, the potassium bromate solution, the 3.00mL concentration that add 10.00mL concentration then and be 0.06mol/L are 5 * 10
-3The YD 30 of mol/L (EDTA) solution and 2.50mL concentration are the H of 0.5mol/L
2SO
4Solution; Constant volume; Obtain treating the test sample solution A, the o-aminophthalylhydrazide analytic liquid and the 2.00mL of 2.00mL step 1 preparation treated that the test sample solution A joins in the wink maidenliness attitude Chemiluminescence Apparatus successively measure the glow peak signal that record reacts; Read peak area Y, bring peak area Y into concentration X that regression equation promptly obtains nitrite then
1Three, the mensuration of nitrate salt: get the extracting solution that the 20mL aforesaid method extracts from sauerkraut; Add 5mL ammonia property buffered soln, 0.2g zinc powder and 0.4mL cadmium chloride solution; Zinc powder and Cadmium chloride fine powder mass ratio are 1: 2, the 40min that under oscillation frequency 180 commentaries on classics/min, 50 ℃ of water bath with thermostatic control conditions, vibrates then, filter, behind the constant volume solution B; Get the 10.00mL solution B then, the potassium bromate solution, the 3.00mL concentration that add 10.00mL concentration again and be 0.06mol/L are 5 * 10
-3The EDTA solution of mol/L and 2.50mL concentration are the H of 0.5mol/L
2SO
4Solution, constant volume obtains treating the test sample solution C; The o-aminophthalylhydrazide analytic liquid of 2.00mL step 1 preparation and 2.00mL are treated that the test sample solution C joins in the wink maidenliness attitude Chemiluminescence Apparatus successively to be measured; The glow peak signal of record reaction reads peak area Y, brings peak area Y into regression equation calculation then and goes out X
2, with (X
2-X
1The concentration that promptly obtains nitrate salt is calculated in) * 1.232; Nitrate salt and nitrite detection in the sauerkraut have promptly been accomplished; Wherein step 2 and three described regression equations: when instrument parameter is chosen as negative high voltage is 325V and 5.74 * 10
7≤Y≤1.13 * 10
9The time bring Y into Y=4.66 * 10
6+ 1.08 * 10
9X, r=0.9947 is 300V and 2.11 * 10 when instrument parameter is chosen as negative high voltage
8≤Y≤1.14 * 10
9The time bring Y into Y=2.02 * 10
7+ 9.38 * 10
6X, r=0.9930.
Above-mentioned detection method is utilized zinc-cadmium water-bath vibration reduction nitrate salt, and the nitrate reduction rate reaches 92.65%, detection method have simple, the consuming time weak point of technology (40min), highly sensitive, linearity range is wide, the simple advantage of plant and instrument.
Embodiment
Embodiment one: the method for nitrate salt and nitrite of extracting in this embodiment in the sauerkraut is undertaken by following step: sauerkraut is rubbed; Getting the sauerkraut of 1g after rub handling then joins 2.5mL borax saturated solution and stirs; Add 10mL zero(ppm) water, then at extracting through microwave heating 5min, the temperature of microwave heating is 80 ℃; Adding potassium ferrocyanide solution and the 1mL concentration that 1mL concentration is 0.25mol/L again is the acetic acid zinc solution of 1mol/L; Constant volume, static filtration is collected filtrating and is obtained extracting solution; Promptly accomplish nitrate salt and nitrite extraction in the sauerkraut.
Nitrate and nitrite recovery rate are all more than 90% in the present embodiment.Recording the content that extracts nitrite in the sauerkraut is 3.95mg/kg, and (2.79mg/kg) compares with National Standard Method, improved 41.6%.
Embodiment two: what this embodiment and embodiment one were different is: the time of said rubbing sauerkraut is 2min, and used rotating speed is 1400r/min.
Embodiment three: detect in the sauerkraut nitrate salt in this embodiment and accomplish by following step with nitrite: one, the preparation of o-aminophthalylhydrazide analytic liquid: absorption 7.5mL concentration is 1 * 10
-3The mol/L luminol solution uses concentration to obtain the o-aminophthalylhydrazide analytic liquid as the NaOH solution constant volume 50mL of 0.25mol/L; Two, the mensuration of nitrite: get the extracting solution that 5.00mL extracts from sauerkraut by embodiment one said method, the potassium bromate solution, the 3.00mL concentration that add 10.00mL concentration then and be 0.06mol/L are 5 * 10
-3The YD 30 of mol/L (EDTA) solution and 2.50mL concentration are the H of 0.5mol/L
2SO
4Solution; Constant volume 50mL; Obtain treating the test sample solution A, the o-aminophthalylhydrazide analytic liquid and the 2.00mL of 2.00mL step 1 preparation treated that the test sample solution A joins in the wink maidenliness attitude Chemiluminescence Apparatus successively measure the glow peak signal that record reacts; Read peak area Y, bring peak area Y into concentration X that regression equation promptly obtains nitrite then
1Three, the mensuration of nitrate salt: get the extracting solution that 20mL embodiment one said method is extracted from sauerkraut; Add 5mL ammonia property buffered soln, 0.2g zinc powder and 0.4mL cadmium chloride solution; Zinc powder and Cadmium chloride fine powder mass ratio are 1: 2, the 40min that under oscillation frequency 180 commentaries on classics/min, 50 ℃ of water bath with thermostatic control conditions, vibrates then, filter, behind the constant volume 50mL solution B; Get the 10.00mL solution B then, the potassium bromate solution, the 3.00mL concentration that add 10.00mL concentration again and be 0.06mol/L are 5 * 10
-3The EDTA solution of mol/L and 2.50mL concentration are the H of 0.5mol/L
2SO
4Solution, constant volume 50mL treats the test sample solution C; The o-aminophthalylhydrazide analytic liquid of 2.00mL step 1 preparation and 2.00mL are treated that the test sample solution A joins in the wink maidenliness attitude Chemiluminescence Apparatus successively to be measured; The glow peak signal of record reaction reads peak area Y, brings peak area Y into regression equation calculation then and goes out X
2, with (X
2-X
1The concentration that promptly obtains nitrate salt is calculated in) * 1.232; Nitrate salt and nitrite detection in the sauerkraut have promptly been accomplished; Wherein step 2 and three described regression equations: when instrument parameter is chosen as negative high voltage is 325V and 5.74 * 10
7≤Y≤1.13 * 10
9The time bring Y into Y=4.66 * 10
6+ 1.08 * 10
9X, r=0.9947 is 300V and 2.11 * 10 when instrument parameter is chosen as negative high voltage
8≤Y≤1.14 * 10
9The time bring Y into Y=2.02 * 10
7+ 9.38 * 10
6X, r=0.9930.
The content that this method detects nitrite in the sauerkraut is 2.84mg/kg,, 11 replicate(determination) results' RSD is 2.90%, and recovery of standard addition is 101.00%, and nitrate content is 7.32mg/kg.
Change into the effect of nitrite through following this embodiment of verification experimental verification nitrate salt: get 20mL nitrate salt standardized solution (solution is equivalent to 5 μ g Sodium Nitrites for every milliliter) in Erlenmeyer flask; Adding 5mL ammonia property buffered soln (pH=9.6～9.7) and solid-to-liquid ratio are 1: 2 zinc powder and (1.5%) cadmium chloride solution; 50 ℃ of temperature; Under the condition of oscillation frequency 180 commentaries on classics/min, vibration 40min.Calculating the nitrate reduction rate is 92.65%, and meeting the nitrate reduction rate should be greater than 90% requirement.
Regression equation can obtain as follows in this embodiment: sulfuric acid concentration is 0.05mol/L, and potassium bromate concentration is 0.06mol/L, and o-aminophthalylhydrazide concentration is 1.50 * 10
-4Mol/L, NaOH concentration are under the 0.25mol/L condition, and the relative luminous intensity of this system is maximum.NO
2 -Concentration is in 0.001～0.1mg/L scope, and obtaining corresponding nitrite typical curve is Y=4.66 * 10
6+ 1.08 * 10
9X (r=0.9947), NO
2 -Concentration is in 0.1～10mg/L scope, and obtaining corresponding nitrite typical curve is Y=2.02 * 10
7+ 9.38 * 10
6X (r=0.9930).Nitrite is good linear relationship with luminous intensity (area) in 0.001～10mg/L scope.
Embodiment four: what this embodiment and embodiment three were different is: pH value=9.6～9.7 of the described ammonia property buffered soln of step 3.Other step is identical with embodiment three with parameter.
Claims (2)
1. the method for nitrate salt and nitrite in the detection sauerkraut, it is characterized in that detecting in the sauerkraut nitrate salt and nitrite and accomplish by following step: one, the preparation of o-aminophthalylhydrazide analytic liquid: drawing 7.5mL concentration is 1 * 10
-3The mol/L luminol solution uses concentration to obtain the o-aminophthalylhydrazide analytic liquid as the NaOH solution constant volume of 0.25mol/L; Two, the mensuration of nitrite: get the extracting solution that 5.00mL extracts from sauerkraut by the said method of claim 1, the potassium bromate solution, the 3.00mL concentration that add 10.00mL concentration then and be 0.06mol/L are 5 * 10
-3The edta solution of mol/L and 2.50mL concentration are the H of 0.5mol/L
2SO
4Solution; Constant volume; Obtain treating the test sample solution A, the o-aminophthalylhydrazide analytic liquid and the 2.00mL of 2.00mL step 1 preparation treated that the test sample solution A joins in the wink maidenliness attitude Chemiluminescence Apparatus successively measure the glow peak signal that record reacts; Read peak area Y, bring peak area Y into concentration X that regression equation promptly obtains nitrite then
1Three, the mensuration of nitrate salt: get the extracting solution that the said method of 20mL claim 1 is extracted from sauerkraut; Add 5mL ammonia property buffered soln, 0.2g zinc powder and 0.4ml cadmium chloride solution; Zinc powder and Cadmium chloride fine powder mass ratio are 1: 2, the 40min that under oscillation frequency 180 commentaries on classics/min, 50 ℃ of water bath with thermostatic control conditions, vibrates then, filter, behind the constant volume solution B; Get the 10.00mL solution B then, the potassium bromate solution, the 3.00mL concentration that add 10.00mL concentration again and be 0.06mol/L are 5 * 10
-3The EDTA solution of mol/L and 2.50mL concentration are the H of 0.5mol/L
2SO
4Solution, constant volume obtains treating the test sample solution C; The o-aminophthalylhydrazide analytic liquid of 2.00mL step 1 preparation and 2.00mL are treated that the test sample solution C joins in the wink maidenliness attitude Chemiluminescence Apparatus successively to be measured; The glow peak signal of record reaction reads peak area Y, brings peak area Y into regression equation calculation then and goes out X
2, with (X
2-X
1The concentration that promptly obtains nitrate salt is calculated in) * 1.232; Nitrate salt and nitrite detection in the sauerkraut have promptly been accomplished; Wherein step 2 and three described regression equations: when instrument parameter is chosen as negative high voltage is 325V and 5.74 * 10
7≤Y≤1.13 * 10
9The time bring Y into Y=4.66 * 10
6+ 1.08 * 10
9X, r=0.9947 is 300V and 2.11 * 10 when instrument parameter is chosen as negative high voltage
8≤Y≤1.14 * 10
9The time bring Y into Y=2.02 * 10
7+ 9.38 * 10
6X, r=0.9930.
2. the method for nitrate salt and nitrite in the detection sauerkraut according to claim 1 is characterized in that pH value=9.6～9.7 of the described ammonia property buffered soln of step 3.
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| CN104330367B (en) * | 2013-11-19 | 2017-01-18 | 上海绿帝环保科技有限公司 | Rapid detection agent for nitrate |
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| CN1320816A (en) * | 2000-04-24 | 2001-11-07 | 攀枝花市卫生防疫站 | Rapid detection method of nitrite in food |
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| 中华人民共和国卫生部.食品安全国家标准 食品中亚硝酸盐与硝酸盐的测定.《中华人民共和国国家标准GB5009.33-2010》.2010,第8页第11.2，11.3节，第5页第9节. * |
| 李健.微波法提取酸菜中硝酸盐及亚硝酸盐工艺研究.《哈尔滨商业大学学报》.2009,第25卷(第6期),685-687. * |
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