CN102008755A - Polyethylene terephthalate and polyurethane composite artificial blood vessel and preparation thereof - Google Patents
Polyethylene terephthalate and polyurethane composite artificial blood vessel and preparation thereof Download PDFInfo
- Publication number
- CN102008755A CN102008755A CN2010105652484A CN201010565248A CN102008755A CN 102008755 A CN102008755 A CN 102008755A CN 2010105652484 A CN2010105652484 A CN 2010105652484A CN 201010565248 A CN201010565248 A CN 201010565248A CN 102008755 A CN102008755 A CN 102008755A
- Authority
- CN
- China
- Prior art keywords
- blood vessel
- artificial blood
- polyethylene terephthalate
- polyurethane
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a polyethylene terephthalate and polyurethane composite artificial blood vessel and a preparation method thereof, belonging to the field of biomedical engineering. The artificial blood vessel is prepared from polyethylene terephthalate and polyurethane by means of an electrostatic spinning device with a stirrer. The prepared artificial blood vessel is a tubular object with a microporous structure. The polyurethane added in the artificial blood vessel can improve the elasticity of the blood vessel under the condition of not damaging the strength of the polyethylene terephthalate material, increase the compliance of the artificial blood vessel and achieve the requirements of clinical use. The polyethylene terephthalate and polyurethane composite artificial blood vessel has the advantages of high strength, large elasticity and compliance, good biocompatibility, strong anticoagulant property and large cell adhesion, and can meet the requirements of the clinical use of the artificial blood vessels with large diameter and small diameter. The preparation process is simple and practical, and applicable to preparing the artificial blood vessels with various different requirements on mechanical properties.
Description
Technical field
The present invention relates to a kind of polyethylene terephthalate and polyurethane composite artificial blood vessel and preparation method thereof, belong to biomedical engineering field.
Background technology
The intravital blood circulation of people is each conveyed nutrition and oxygen, and the intravital refuse of collector is also got rid of it in body.Blood vessel is responsible for heart and its hetero-organization are coupled together, and the mobile passage that provides of blood is provided, adjusts the local equilibrium of blood circulation.When human vas occurred damaged or stops up, blood can't continue as partial tissue nutrition and oxygen are provided, and causes tissue necrosis, finally threatens the life of human body.Thereby blood vessel is because arteriosclerosis, during aging or damaged cisco unity malfunction, needs to adopt the artificial blood vessel to replace, put up a bridge or surgical operation such as intervention is treated.
Continue nineteen fifty-two Voorhees success use loose structure polyvinyl artificial blood vessel in animal body after, many researchers work out various new materials and various novel processing method, and are applied among the artificial blood vessel, have promoted the fast development of artificial blood vessel's research.At present, the heavy caliber of terylene and expanded polytetrafluoroethylsealing preparation (＞6mm) artificial blood vessel uses clinically, and obtained certain effect.
Terylene is to be obtained by melt spinning method by polyethylene terephthalate.The method that (woven or knitting) weaved in use is prepared into the heavy caliber artificial blood vessel with polyester filament.Terylene has the requirement that very high brute force can satisfy artificial blood vessel's brute force, but elasticity is relatively poor, and the artificial blood vessel of preparation elasticity and compliance diametrically can't be complementary with the human body real blood vessels, in use causes hamartoplasia easily and makes blood vessel blockage.Adopting woven method to prepare terylene artificial blood vessel main purpose is in order to improve terylene artificial blood vessel's elasticity, to increase the adhesive capacity of blood vessel surface porosity and raising endotheliocyte.The dacron-made artificial blood vessel that obtains by method for weaving can deform under the effect of external force, increases elasticity of blood vessels.But the improvement degree of blood vessel on elasticity that is obtained by method for weaving is limited, still can't satisfy the requirement of small-caliber artificial blood vessel use and the target of the long-time patency rate of the further heavy caliber artificial blood vessel of raising.In order further to improve terylene artificial blood vessel's long-time patency rate, the probability of hamartoplasia appears after the reduction blood vessel transplantation, and the elasticity and the compliance that increase the terylene artificial blood vessel by additive method are very necessary.
Summary of the invention
At above problem, the objective of the invention is to prepare a kind of artificial blood vessel with favorable elasticity, reach the requirement of clinical use.Technical scheme of the present invention to achieve these goals is:
A kind of polyethylene terephthalate and polyurethane composite artificial blood vessel, the artificial blood vessel is meant the tube with microcellular structure that is formed by the electrostatic spinning apparatus preparation by polyethylene terephthalate and polyurethane mixed solution, wherein, artificial blood vessel's component is
Polyethylene terephthalate 60-95 part
Polyurethane 5-40 part
Wherein, described polyurethane be CorethaneTM,
80A,
90A, Chronoflex AR, Chronoflex CL,
Elast Eon, Cardiomat or Avcothane.
Described artificial blood vessel's internal diameter is 1mm～30mm;
Described artificial blood vessel's wall thickness is 100 μ m～1.5mm;
The preparation process of polyethylene terephthalate and polyurethane composite artificial blood vessel is as follows:
The preparation of a solution:
Polyethylene terephthalate 60-95 part and polyurethane 5-40 part are dissolved in the hexafluoroisopropanol solvent jointly, are 5～12% spinning solution through being stirred well to fully dissolving, making mass fraction;
B artificial blood vessel's preparation:
To be encased in the electrostatic spinning apparatus that has agitator through the spinning solution of a preparation, adopt drum die as receiving device, spinning solution is stirred in the process that device stirs through the speed ejection of spinning-nozzle with 0.1-2ml/h, entering voltage is 15-25Kv, distance is the high-tension electricity place of 50-300mm, forms the composite fibre of polyethylene terephthalate and polyurethane, is collected on the drum die of doing rotation and straight reciprocating motion, formation has artificial blood vessel's structure of micropore
Wherein, described composite fibre diameter is 50nm～100 μ m;
The rotating speed of described agitator is 10～50 commentaries on classics/min;
The rotary speed of described drum die is 10～100 commentaries on classics/min;
The speed of the straight reciprocating motion of described drum die is 100mm/min～500mm/min.
The axis normal of described drum die is in the nozzle of electrospinning device.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 10～30 ℃ the distilled water and soak 3～6h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 40～60 ℃ of vacuum conditions and carries out drying, the artificial blood vessel who promptly makes polyethylene terephthalate and polyurethane behind dry 6～10h.
Owing to adopt above technical scheme, the polyethylene terephthalate and the polyurethane composite artificial blood vessel Main Ingredients and Appearance of the present invention's preparation are polyethylene terephthalate and polyurethane, wherein polyethylene terephthalate materials is a kind of material with fine brute force and fine wearability, and polyurethane material is a kind of material with favorable elasticity, two kinds of material mixing with different mechanical characteristics are prepared into the shortcoming that product can remedy the homogenous material product together, therefore the polyethylene terephthalate and the polyurethane artificial blood vessel of the present invention's preparation have better elastic and mechanical property, the requirement of clinical use can be satisfied, the difficult problem of the clinical middle terylene artificial blood vessel poor flexibility of using can be overcome.In artificial blood vessel's course of processing, use agitator spinning solution to be stirred on one side, Yi Bian carry out spinning, realized the synchronous of two kinds of material mixing of polyethylene terephthalate and polyurethane and spinning, keep two kinds of material mixing even, prevent layering.The speed controlling of agitator both can play at 10～50 commentaries on classics/min that polymer mixed was acted on uniformly, also can not influence spinning speed simultaneously.Both contained polyethylene terephthalate materials in the every nanofiber of preparation, and also contained simultaneously and have fine elastic polyurethane with fine intensity.How much polyurethane content has determined elasticity of blood vessels among the artificial blood vessel.Polyurethane content is high more in the blood vessel, and elasticity of blood vessels is good more.But along with the increase of polyurethane content, the hot strength of blood vessel descends, so the content of polyurethane is not suitable for surpassing 40% among the artificial blood vessel.Mainly with replacing the natural blood vessel that damages in the human body clinically, artificial blood vessel's internal diameter must be consistent with the diameter of human body natural's blood vessel the artificial blood vessel of preparation.The diameter of main dependence swing roller is controlled the internal diameter of blood vessel among the present invention, reaches the artificial blood vessel of preparation different inner diameters by the drum die of changing different-diameter.The thickness of blood vessel wall is by deciding process time.In the course of processing, the constant airspeed of spinning, the spinning time is long more, and the polymer that cylinder surface receives is many more, and artificial blood vessel's wall thickness is also thick more.The wall thickness of human body real blood vessels is generally 100 μ m～1.5mm, by control spinning speed and the spinning time acquisition thickness similar to the human vas wall thickness.The drum die of rotation and straight reciprocating motion is the receiving device of artificial blood vessel, has directly determined the shape of blood vessel and the uniformity coefficient of wall thickness.The rotating speed of cylinder is that the speed of 10～100 commentaries on classics/min and straight reciprocating motion is that 100mm/min～500mm/min can guarantee to obtain to have the uniform artificial blood vessel of wall thickness.Because the present invention adopts the polyester material of clinical use and has the polyurethane material of good biocompatibility and anticoagulant property, therefore the composite artificial blood vessel of preparation has good biocompatibility, anticoagulant property, cell adhesion, elasticity and compliance can satisfy the requirement of clinical use.
Polyethylene terephthalate of the present invention and polyurethane artificial blood vessel have big, the good biocompatibility of powerful height, elasticity and compliance, and anticoagulant property is strong, and cell adhesion is big, can satisfy the requirement of the clinical use of large and small bore artificial blood vessel.The preparation that preparation technology of the present invention is simple, practical, the various different mechanical properties of adaptation require artificial blood vessels.
Description of drawings
Accompanying drawing is preparation polyethylene terephthalate and polyurethane artificial blood vessel's an electrostatic spinning apparatus structural representation
The specific embodiment
Below in conjunction with drawings and Examples the present invention is described in further detail.
A kind of polyethylene terephthalate and polyurethane composite artificial blood vessel, the artificial blood vessel is meant the tube with microcellular structure that is formed by the electrostatic spinning apparatus preparation by polyethylene terephthalate and polyurethane mixed solution, wherein, artificial blood vessel's component is
Polyethylene terephthalate 60-95 part
Polyurethane 5-40 part
Wherein, described polyurethane be CorethaneTM,
80A,
90A, Chronoflex AR, Chronoflex CL,
Elast Eon, Cardiomat or Avcothane.
The preparation process of polyethylene terephthalate and polyurethane composite artificial blood vessel is as follows:
The preparation of a solution:
Polyethylene terephthalate 60-95 part and polyurethane 5-40 part are dissolved in the hexafluoroisopropanol solvent jointly, are 5～12% spinning solution 4 through being stirred well to fully dissolving, making mass fraction;
B artificial blood vessel's preparation:
To be encased in the solution tank 2 of the electrostatic spinning apparatus that has agitator 3 through the spinning solution 4 of a preparation, agitator 3 places solution tank 2, agitator 3 stirs spinning solution 4 with the rotating speed of 10～50 commentaries on classics/min, make polyethylene terephthalate and polyurethane uniform mixing in the spinning solution 4, artificial blood vessel's structure formation adopts drum die 7 as receiving device, drum die 7 is positioned at the below of electrostatic spinning apparatus nozzle 5, its axis normal is in the nozzle 5 of electrostatic spinning apparatus, rotary speed 10～100 commentaries on classics/min of drum die 7, the straight reciprocating motion speed of drum die 7 is 100mm/min～500mm/min, spinning solution 4 is released through nozzle 5 by micro-injection pump 1 speed with 0.1-2ml/h from solution tank 2 in the process that agitator 3 stirs, entering voltage is 15-25Kv, distance is the high-tension electricity place 6 of 50-300mm, form the composite fibre of polyethylene terephthalate and polyurethane, composite fibre is collected on the drum die 7 of doing rotation and straight reciprocating motion, the composite fibre diameter of collecting is 50nm～100 μ m, forming internal diameter is that 1mm～30mm and wall thickness are 100 μ m～1.5mm artificial blood vessel structure, the internal diameter of artificial blood vessel's structure is directly decided by the diameter of drum die, the wall thickness of artificial blood vessel's structure is relevant with the time, under the constant precondition of spinning speed, the time of drum die collection fiber is long more, and the wall thickness that obtains artificial blood vessel's structure is thick more.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 10～30 ℃ the distilled water and soak 3～6h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 40～60 ℃ of vacuum conditions and carries out drying, the artificial blood vessel who promptly makes polyethylene terephthalate and polyurethane behind dry 6～10h.。
Preparation method below in conjunction with above-mentioned polyethylene terephthalate and polyurethane composite artificial blood vessel is further elaborated with regard to specific embodiment.
Specific embodiment
Embodiment one
A takes by weighing 4.75g polyethylene terephthalate and 0.25g polyurethane (CorethaneTM) on the precise electronic balance, fully be dissolved in the 95g hexafluoroisopropanol, and acquisition concentration is 5% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 95/5.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 10 commentaries on classics/min, select for use diameter be the drum die of 1mm as gathering-device, the rotary speed of setting drum die is 10 commentaries on classics/min, the speed of straight reciprocating motion is 100mm/min.It is 15KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 0.1ml/h, electric field distance is in the high voltage electric field of 50mm, it is the composite fibre 6h of 100 μ m that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 1mm, and wall thickness is artificial blood vessel's structure of 100 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 10 ℃ the distilled water and soak 3h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 40 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 6h.
Embodiment two
A on the precise electronic balance, take by weighing 8g polyethylene terephthalate and 2g polyurethane (
80A), fully be dissolved in the 90g hexafluoroisopropanol, acquisition concentration is 10% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 80/20.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 50 commentaries on classics/min, selecting diameter for use is that the drum die of 30mm is as gathering-device, the rotary speed of setting drum die is 100 commentaries on classics/min, and the speed of straight reciprocating motion is 500mm/min.It is 25KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 0.1ml/h, electric field distance is in the high voltage electric field of 300mm, it is the composite fibre 6h of 50nm that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 30mm, and wall thickness is artificial blood vessel's structure of 100 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 30 ℃ the distilled water and soak 6h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 60 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 10h.
Embodiment three
A on the precise electronic balance, take by weighing 8g polyethylene terephthalate and 2g polyurethane (
90A), fully be dissolved in the 90g hexafluoroisopropanol, acquisition concentration is 10% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 80/20.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 30 commentaries on classics/min, selecting diameter for use is that the drum die of 10mm is as gathering-device, the rotary speed of setting drum die is 50 commentaries on classics/min, and the speed of straight reciprocating motion is 300mm/min.It is 20KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 0.5ml/h, electric field distance is in the high voltage electric field of 200mm, it is the composite fibre 10h of 500nm that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 10mm, and wall thickness is artificial blood vessel's structure of 600 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 20 ℃ the distilled water and soak 4h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 50 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 8h.
Embodiment four
A takes by weighing 7.2g polyethylene terephthalate and 4.8g polyurethane (Chronoflex AR) on the precise electronic balance, fully be dissolved in the 88g hexafluoroisopropanol, and acquisition concentration is 12% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 60/40.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 30 commentaries on classics/min, select for use diameter be the drum die of 6mm as gathering-device, the rotary speed of setting drum die is 50 commentaries on classics/min, the speed of straight reciprocating motion is 100mm/min.It is 20KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 0.5ml/h, electric field distance is in the high voltage electric field of 200mm, it is the composite fibre 24h of 500 μ m that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 6mm, and wall thickness is artificial blood vessel's structure of 1.5mm.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 30 ℃ the distilled water and soak 6h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 50 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 8h.
Embodiment five
A takes by weighing 4.75g polyethylene terephthalate and 0.25g polyurethane (Chronoflex CL) on the precise electronic balance, fully be dissolved in the 95g hexafluoroisopropanol, and acquisition concentration is 5% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 95/5.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 20 commentaries on classics/min, selecting diameter for use is that the drum die of 10mm is as gathering-device, the rotary speed of setting drum die is 20 commentaries on classics/min, and the speed of straight reciprocating motion is 300mm/min.It is 25KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 1ml/h, electric field distance is in the high voltage electric field of 150mm, it is the composite fibre 16h of 400nm that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 10mm, and wall thickness is artificial blood vessel's structure of 900 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 20 ℃ the distilled water and soak 3h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 40 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 10h.
Embodiment six
A on the precise electronic balance, take by weighing 8g polyethylene terephthalate and 2g polyurethane (
), fully being dissolved in the 90g hexafluoroisopropanol, acquisition concentration is 10% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 80/20.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 10 commentaries on classics/min, selecting diameter for use is that the drum die of 20mm is as gathering-device, the rotary speed of setting drum die is 10 commentaries on classics/min, and the speed of straight reciprocating motion is 100mm/min.It is 20KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 1ml/h, electric field distance is in the high voltage electric field of 150mm, it is the composite fibre 10h of 50 μ m that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 20mm, and wall thickness is artificial blood vessel's structure of 300 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 10 ℃ the distilled water and soak 6h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 50 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 10h.
Embodiment seven
A takes by weighing 4.75g polyethylene terephthalate and 0.25g polyurethane (Elast Eon) on the precise electronic balance, fully be dissolved in the 95g hexafluoroisopropanol, and acquisition concentration is 5% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 95/5.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 10 commentaries on classics/min, select for use diameter be the drum die of 1mm as gathering-device, the rotary speed of setting drum die is 10 commentaries on classics/min, the speed of straight reciprocating motion is 100mm/min.It is 15KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 0.1ml/h, electric field distance is in the high voltage electric field of 50mm, it is the composite fibre 6h of 100 μ m that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 1mm, and wall thickness is artificial blood vessel's structure of 100 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 10 ℃ the distilled water and soak 3h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 40 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 6h.
Embodiment eight
A takes by weighing 7.2g polyethylene terephthalate and 4.8g polyurethane (Cardiomat) on the precise electronic balance, fully be dissolved in the 88g hexafluoroisopropanol, and acquisition concentration is 12% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 60/40.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 20 commentaries on classics/min, select for use diameter be the drum die of 6mm as gathering-device, the rotary speed of setting drum die is 20 commentaries on classics/min, the speed of straight reciprocating motion is 200mm/min.It is 20KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 0.5ml/h, electric field distance is in the high voltage electric field of 150mm, it is the composite fibre 8h of 300nm that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 6mm, and wall thickness is artificial blood vessel's structure of 150 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 30 ℃ the distilled water and soak 4h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 50 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 10h.
Embodiment nine
A takes by weighing 4.75g polyethylene terephthalate and 0.25g polyurethane (Avcothane) on the precise electronic balance, fully be dissolved in the 95g hexafluoroisopropanol, and acquisition concentration is 5% spinning solution.Wherein the mass ratio between polyethylene terephthalate and the polyurethane is 95/5.
The spinning solution of b preparation joins electrostatic spinning with in the solution tank, agitator stirs spinning solution with the rotating speed of 10 commentaries on classics/min, selecting diameter for use is that the drum die of 10mm is as gathering-device, the rotary speed of setting drum die is 10 commentaries on classics/min, and the speed of straight reciprocating motion is 100mm/min.It is 20KV that micro-injection pump is pushed into voltage with the spinning solution that stirs through nozzle with the fltting speed of 0.8ml/h, electric field distance is in the high voltage electric field of 300mm, it is the composite fibre 8h of 250nm that drum die receives the diameter that comes out through high voltage electric field continuously, the formation internal diameter is 10mm, and wall thickness is artificial blood vessel's structure of 250 μ m.
C takes off the artificial blood vessel's structure after drum die is collected, be placed in 30 ℃ the distilled water and soak 6h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 60 ℃ of vacuum conditions and carries out drying, promptly makes the artificial blood vessel of polyethylene terephthalate and polyurethane behind the dry 8h.
Claims (4)
1. polyethylene terephthalate and polyurethane composite artificial blood vessel, it is characterized in that: the artificial blood vessel is meant the tube with microcellular structure that is formed by the electrostatic spinning apparatus preparation by polyethylene terephthalate and polyurethane mixed solution, wherein, artificial blood vessel's component is
Polyethylene terephthalate 60-95 part
Polyurethane 5-40 part
Wherein, described polyurethane be CorethaneTM,
80A,
90A, Chronoflex AR, Chronoflex CL,
Elast Eon, Cardiomat or Avcothane.
2. polyethylene terephthalate as claimed in claim 1 and polyurethane composite artificial blood vessel is characterized in that: described artificial blood vessel's internal diameter is 1mm～30mm, and artificial blood vessel's wall thickness is 100 μ m～1.5mm;
3. the preparation method of polyethylene terephthalate and polyurethane composite artificial blood vessel, it is characterized in that: artificial blood vessel's preparation process is as follows:
The preparation of a solution:
Polyethylene terephthalate 60-95 part and polyurethane 5-40 part are dissolved in the hexafluoroisopropanol solvent jointly, are 5～12% spinning solution (4) through being stirred well to fully dissolving, making mass fraction;
The preparation of b artificial blood vessel structure:
Will be through the spinning solution of a preparation, (4) be encased in and have agitator, (3) in the electrostatic spinning apparatus, adopt drum die, (7) as receiving device, spinning solution, (4) be stirred device, (3) in the process of Jiao Baning through spinning-nozzle, (5) speed with 0.1-2ml/h sprays, entering voltage is 15-25Kv, distance is the high voltage electric field of 50-300mm, (6), form the composite fibre of polyethylene terephthalate and polyurethane, be collected in the drum die of doing rotation and straight reciprocating motion, (7) on, form artificial blood vessel's structure
Wherein, described composite fibre diameter is 50nm～100 μ m;
The rotating speed of described agitator is 10～50 commentaries on classics/min;
The rotary speed of described drum die is 10～100 commentaries on classics/min;
The speed of the straight reciprocating motion of described drum die is 100mm/min～500mm/min.
C will take off through the artificial blood vessel's structure after drum die (7) is collected, be placed in 10～30 ℃ the distilled water and soak 3～6h, use fresh distilled water to replace original soak every 1h, artificial blood vessel after the immersion puts under 40～60 ℃ of vacuum conditions and carries out drying, the artificial blood vessel who promptly makes polyethylene terephthalate and polyurethane behind dry 6～10h.
4. the preparation method of a kind of polyethylene terephthalate as claimed in claim 2 and polyurethane composite artificial blood vessel is characterized in that: the axis normal of described drum die (7) is in the nozzle (5) of electrospinning device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010565248 CN102008755B (en) | 2010-11-25 | 2010-11-25 | Polyethylene terephthalate and polyurethane composite artificial blood vessel and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010565248 CN102008755B (en) | 2010-11-25 | 2010-11-25 | Polyethylene terephthalate and polyurethane composite artificial blood vessel and preparation thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102008755A true CN102008755A (en) | 2011-04-13 |
| CN102008755B CN102008755B (en) | 2013-07-03 |
Family
ID=43839206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010565248 Active CN102008755B (en) | 2010-11-25 | 2010-11-25 | Polyethylene terephthalate and polyurethane composite artificial blood vessel and preparation thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102008755B (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156707A (en) * | 2013-03-07 | 2013-06-19 | 武汉纺织大学 | Artificial blood vessel preparing device |
| WO2014145864A1 (en) | 2013-03-15 | 2014-09-18 | Nanofiber Solutions, Llc | Biocompatible fiber textiles for implantation |
| CN104088050A (en) * | 2014-06-30 | 2014-10-08 | 太仓天龙化纤有限公司 | Wear-resisting anti-bacteria stretch yarn |
| CN105311674A (en) * | 2015-11-18 | 2016-02-10 | 武汉纺织大学 | Preparation method of high-elasticity crease-resistant artificial blood vessel |
| CN105839203A (en) * | 2016-04-28 | 2016-08-10 | 中国工程物理研究院化工材料研究所 | Three-dimensional porous yarn prepared through electro-spinning technology and preparation method of three-dimensional porous yarn |
| US9737632B2 (en) | 2013-09-25 | 2017-08-22 | Nanofiber Solutions, Inc. | Fiber scaffolds for use creating implantable structures |
| US9884027B2 (en) | 2012-01-12 | 2018-02-06 | Nanofiber Solutions, Inc. | Nanofiber scaffolds for biological structures |
| WO2018112203A1 (en) * | 2016-12-14 | 2018-06-21 | eLum Technologies, Inc. | Electrospun stents, flow diverters, and occlusion devices and methods of making the same |
| US10227568B2 (en) | 2011-03-22 | 2019-03-12 | Nanofiber Solutions, Llc | Fiber scaffolds for use in esophageal prostheses |
| US10562225B2 (en) | 2011-11-21 | 2020-02-18 | Nanofiber Solutions, Llc | System for manufacturing fiber scaffolds for use in tracheal prostheses |
| US10898608B2 (en) | 2017-02-02 | 2021-01-26 | Nanofiber Solutions, Llc | Methods of improving bone-soft tissue healing using electrospun fibers |
| US10953097B2 (en) | 2015-11-02 | 2021-03-23 | Nanofiber Solutions. Llc | Electrospun fibers having contrast agents and methods of making the same |
| US11576927B2 (en) | 2018-12-11 | 2023-02-14 | Nanofiber Solutions, Llc | Methods of treating chronic wounds using electrospun fibers |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545212A (en) * | 1991-12-18 | 1996-08-13 | Terumo Kabushiki Kaisha | Artificial blood vessel |
| CN201341971Y (en) * | 2009-01-21 | 2009-11-11 | 上海契斯特医疗科技公司 | Thin dacron-made artificial blood vessel with interval lines |
| CN101785875A (en) * | 2010-02-25 | 2010-07-28 | 王深明 | Preparation method of nano superfine fiber vascular prosthesis |
-
2010
- 2010-11-25 CN CN 201010565248 patent/CN102008755B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545212A (en) * | 1991-12-18 | 1996-08-13 | Terumo Kabushiki Kaisha | Artificial blood vessel |
| CN201341971Y (en) * | 2009-01-21 | 2009-11-11 | 上海契斯特医疗科技公司 | Thin dacron-made artificial blood vessel with interval lines |
| CN101785875A (en) * | 2010-02-25 | 2010-07-28 | 王深明 | Preparation method of nano superfine fiber vascular prosthesis |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10227568B2 (en) | 2011-03-22 | 2019-03-12 | Nanofiber Solutions, Llc | Fiber scaffolds for use in esophageal prostheses |
| US10233427B2 (en) | 2011-03-22 | 2019-03-19 | Nanofiber Solutions, Llc | Fiber scaffolds for use in esophageal prostheses |
| US10562225B2 (en) | 2011-11-21 | 2020-02-18 | Nanofiber Solutions, Llc | System for manufacturing fiber scaffolds for use in tracheal prostheses |
| US9884027B2 (en) | 2012-01-12 | 2018-02-06 | Nanofiber Solutions, Inc. | Nanofiber scaffolds for biological structures |
| US11737990B2 (en) | 2012-01-12 | 2023-08-29 | Nfs Ip Holdings, Llc | Nanofiber scaffolds for biological structures |
| US10653635B2 (en) | 2012-01-12 | 2020-05-19 | Nanofiber Solutions, Llc | Nanofiber scaffolds for biological structures |
| CN103156707A (en) * | 2013-03-07 | 2013-06-19 | 武汉纺织大学 | Artificial blood vessel preparing device |
| CN103156707B (en) * | 2013-03-07 | 2015-08-05 | 武汉纺织大学 | A kind of artificial blood vessel preparation facilities |
| EP2971318A4 (en) * | 2013-03-15 | 2016-11-23 | Nanofiber Solutions Inc | Biocompatible fiber textiles for implantation |
| US11246959B2 (en) * | 2013-03-15 | 2022-02-15 | Nanofiber Solutions, Llc | Biocompatible fiber textiles for implantation |
| WO2014145864A1 (en) | 2013-03-15 | 2014-09-18 | Nanofiber Solutions, Llc | Biocompatible fiber textiles for implantation |
| EP2971318B1 (en) * | 2013-03-15 | 2021-07-21 | Nanofiber Solutions, LLC | Biocompatible fiber textiles for implantation |
| US9737632B2 (en) | 2013-09-25 | 2017-08-22 | Nanofiber Solutions, Inc. | Fiber scaffolds for use creating implantable structures |
| CN104088050A (en) * | 2014-06-30 | 2014-10-08 | 太仓天龙化纤有限公司 | Wear-resisting anti-bacteria stretch yarn |
| US10953097B2 (en) | 2015-11-02 | 2021-03-23 | Nanofiber Solutions. Llc | Electrospun fibers having contrast agents and methods of making the same |
| CN105311674A (en) * | 2015-11-18 | 2016-02-10 | 武汉纺织大学 | Preparation method of high-elasticity crease-resistant artificial blood vessel |
| CN105839203B (en) * | 2016-04-28 | 2019-02-12 | 中国工程物理研究院化工材料研究所 | Utilize the three-dimensional porous yarn and preparation method thereof of Electrospinning preparation |
| CN105839203A (en) * | 2016-04-28 | 2016-08-10 | 中国工程物理研究院化工材料研究所 | Three-dimensional porous yarn prepared through electro-spinning technology and preparation method of three-dimensional porous yarn |
| WO2018112203A1 (en) * | 2016-12-14 | 2018-06-21 | eLum Technologies, Inc. | Electrospun stents, flow diverters, and occlusion devices and methods of making the same |
| US10898608B2 (en) | 2017-02-02 | 2021-01-26 | Nanofiber Solutions, Llc | Methods of improving bone-soft tissue healing using electrospun fibers |
| US11806440B2 (en) | 2017-02-02 | 2023-11-07 | Nfs Ip Holdings, Llc | Methods of improving bone-soft tissue healing using electrospun fibers |
| US11576927B2 (en) | 2018-12-11 | 2023-02-14 | Nanofiber Solutions, Llc | Methods of treating chronic wounds using electrospun fibers |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102008755B (en) | 2013-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102008755B (en) | Polyethylene terephthalate and polyurethane composite artificial blood vessel and preparation thereof | |
| US11246959B2 (en) | Biocompatible fiber textiles for implantation | |
| CN102430157B (en) | Medical scaffold with inner coating film, and preparation method for medical scaffold | |
| CN101214393B (en) | Nano fibrous tissue engineering blood vessel and preparation thereof | |
| Zhang et al. | Electrospun silk biomaterial scaffolds for regenerative medicine | |
| CN100594948C (en) | Preparing method and use of chitosan-containing nano fibrous tissue recovery support | |
| CN101879330A (en) | Small-caliber silk fibroin tubular material and preparation method thereof | |
| CN106668944B (en) | Three-layer composite small-caliber intravascular stent and preparation method thereof | |
| CN104383606B (en) | A kind of high-strength high-elasticity intravascular stent and preparation method thereof | |
| CN101703796B (en) | Nano fibre artificial vascular graft modifying internal layer and preparation method thereof | |
| CN101708344B (en) | Nanofiber vascular prostheses and preparation method | |
| CN106540327A (en) | A kind of three layers of artificial blood vessel bracket of imitative nature blood vessel and preparation method thereof | |
| CN101264349A (en) | Method for preparing composite artificial blood vessel stent by combined electro-spinning with knitting technique | |
| CN106492273B (en) | A kind of dual enhancing Biodegradable polyester fibrous composite of chitin whisker/chitosan nano fiber and the preparation method and application thereof | |
| CN103266421B (en) | Preparation method of caprolactone lactate copolymer/collagen/chitosan small-caliber intravascular stent | |
| Zhu et al. | Characterization of a co-electrospun scaffold of HLC/CS/PLA for vascular tissue engineering | |
| CN103083720A (en) | Silk fibroin tube and preparation method thereof | |
| CN108642719A (en) | A kind of apparatus and method of Static Spinning integration small-caliber artificial blood vessel holder | |
| CN111265721B (en) | Preparation method of electrostatic spinning double-layer artificial blood vessels with different diameters | |
| CN101785875A (en) | Preparation method of nano superfine fiber vascular prosthesis | |
| Oprea et al. | Electrospun nanofibers for tissue engineering applications | |
| CN107802888A (en) | A kind of preparation method of the nano fiber scaffold of promotion regenerating bone or cartilage | |
| CN109172875A (en) | A kind of preparation method of micron and nanometer composite structure artificial blood vessel | |
| CHEN et al. | Optimization of electrospinning process parameters for tissue engineering scaffolds | |
| CN104831476A (en) | Preparation method for degradable polylactic acid-glycolic acid/polycaprolactone/I-type collagen electrospun fiber membrane material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: NANJING BEILONG MEDICAL APPARATUS AND INSTRUMENT C Free format text: FORMER OWNER: WUHAN TEXTILE UNIVERSITY Effective date: 20150107 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 430073 WUHAN, HUBEI PROVINCE TO: 211100 NANJING, JIANGSU PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20150107 Address after: 211100 Jiangning Science Park, No. 1009 Tianyuan East Road, Jiangning District, Jiangsu, Nanjing Patentee after: Veron Nanjing Medical Devices Co. Address before: 430073 Textile Road, Hongshan District, Hubei, China, No. 1, No. Patentee before: Wuhan Textile University |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20201207 Address after: 315000 1-2, 1 / F, building 3, No. 27, Anju lane, Xiaogang street, Beilun District, Ningbo City, Zhejiang Province Patentee after: Ningbo beiang biomaterials Co., Ltd Address before: 211100 Jiangning Science Park, No. 1009 Tianyuan East Road, Jiangning District, Jiangsu, Nanjing Patentee before: Veron Nanjing Medical Devices Co. |
|
| TR01 | Transfer of patent right |