CN102000147A - Medicament formula and preparation thereof for preventing and curing altitude reaction and coronary heart disease - Google Patents
Medicament formula and preparation thereof for preventing and curing altitude reaction and coronary heart disease Download PDFInfo
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- CN102000147A CN102000147A CN2009101728392A CN200910172839A CN102000147A CN 102000147 A CN102000147 A CN 102000147A CN 2009101728392 A CN2009101728392 A CN 2009101728392A CN 200910172839 A CN200910172839 A CN 200910172839A CN 102000147 A CN102000147 A CN 102000147A
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Abstract
The invention relates to a medicament formula and a preparation thereof for preventing and curing an altitude reaction and a coronary heart disease. The medicament formula comprises 10 to 40g of rhodiola rosea, 60 to 150g of salvia miltiorrhiza, 1 to 5g of ginkgo biloba extract and 0.3 to 1.5g of artificial musk or borneol. The preparation comprises oral solid preparations of dripping pills, troches, capsulas, granules, pills, powder, and the like. The preparation method of dripping pills is characterized by extracting salvianolic acid B of salvia miltiorrhiza extract by adopting a method combining alcohol extraction and ethyl acetate extraction, the purifying method adopts macroporous adsorbent resins, and an eluent is 20% to 60% ethanol. The extraction of salidroside of rhodiola rosea extract adopts 30 to 90% ethanol for heating and backflow, the purifying method adopts the macroporous adsorbent resins, and the eluent is 10 to 50% ethanol. A salvia miltiorrhiza purified matter (salvianolic acid B), a rhodiola rosea purified matter (high-purity salidroside), the ginkgo biloba extract and the artificial musk or borneol are mixed with PEG (polyethyleneglycol) 4000 and PEG 6000 which are 0.5-3 times the amount of the salvia miltiorrhiza purified matter (salvianolic acid B), the rhodiola rosea purified matter (high-purity salidroside), the ginkgo biloba extract and the artificial musk or borneol, the , the mixture is heated for fusion and kept at 60 to 80 DEG C, and the mixture is dropped to a coolant, condensated, screened and dried to obtain the preparation.
Description
Affiliated technical field
The present invention relates to a kind of pharmaceutical formulation and preparation that is used to prevent and treat altitude sickness and coronary heart disease.
Background technology
Altitude sickness is acute high altitude sickness (acute high altitude disease, AHAD), be after the people arrives certain height above sea level, draught head, the oxygen content cause because of height above sea level are few in order to adapt to for health, the variation of air drying etc., and the natural physiological reaction that produces, when height above sea level generally reaches 2700 meters left and right sides, just have altitude sickness.Altitude sickness is the important content of plateau medicine, and the rudiment of plateau medicine produces understanding to altitude sickness and proposes control from people and is starting point.Altitude sickness is a huge threat to entering plateau and short-term inhabitation plateau person rapidly not only, and it also is a serious problem that residence of a specified duration and generation are occupied the plateau person.Along with continually developing of highlands, a large amount of crowds high altitude localities that transfers one's residence to will be arranged, the control of altitude sickness more becomes an outstanding realistic problem and receives publicity.How to take prophylactico-therapeutic measures effectively, just become a central topic of plateau medicine.(CoronaryHeartDISease is common clinically, that easily send out and easy fatal diseases CHD) to coronary heart disease, has brought great harm for the physical and mental health of mid-aged population.Because Radix Rhodiolae has great ischemia resisting, oxygen lack resistant function.In high altitude anoxia Tibet, area and Qinghai, Radix Rhodiolae is one of required medicines commonly used, is widely used in clinical.Radix Rhodiolae extract can reduce myocardial oxygen consumption, under the situation that does not reduce coronary flow, can keep the demand of cardiac muscle to energy, has therefore improved the pump effect rate of heart; Radix Rhodiolae extract has decreased heart rate simultaneously, brings high blood pressure down, reduces effects such as coronary resistance, also is beneficial to anginal alleviation.At present, it is few effectively to treat simultaneously the types of drugs of altitude sickness and angina pectoris.The present invention relates to a kind of pharmaceutical formulation and preparation that is used to prevent and treat altitude sickness and coronary heart disease.
Summary of the invention
The invention provides a kind of pharmaceutical formulation and preparation, be used to prevent and treat altitude sickness and coronary heart disease.
The technical solution adopted for the present invention to solve the technical problems is: pharmaceutical formulation is Radix Rhodiolae 10～40g Radix Salviae Miltiorrhizae 60～150g Folium Ginkgo extract 1～5g artificial Moschus (perhaps Borneolum Syntheticum) 0.3～1.5g.Its preparation comprises: oral solid formulations such as drop pill, tablet, capsule, granule, pill, powder.Oral solid formulation with this formulation is used to prevent and treat altitude sickness and coronary heart disease.
The invention has the beneficial effects as follows that this prescription and preparation thereof also provide the high-quality selection for the coronary heart disease clinical application except alleviating altitude sickness fast.
The specific embodiment
The preparation of example one---drop pill
(1) extraction and purification process of rhodioside and condition
Get the recipe quantity Radix Rhodiolae, pulverize the Radix Rhodiolae medical material, cross sieve No. one.Add 70% ethanol at 1: 8 by solid-liquid ratio, electric jacket heating and refluxing extraction 3 times, each 1h, 200 order filter clothes filter, and merging filtrate reclaims ethanol to there not being the alcohol flavor.Extracting solution centrifugal (centrifugal force: 3200,10min), get upper strata liquid and go up sample in right amount, earlier measure the column volume water elution with 1 times, use 10% ethanol elution of 50ml/1g medical material then, collect 10% ethanol elution, concentrate drying.Get the big glycosides purification of red scape thing.
Research contents:
Take by weighing Radix Rhodiolae coarse powder 20g, add 70% ethanol, pressed solid-liquid ratio 8: 1,85 ℃ of water-bath reflux, extract, 3 times, each 1h, filter paper filtering, merging filtrate reclaims ethanol to not having alcohol flavor, extracting liquid volume 90ml.
Getting the D-101 macroporous resin puts in the beaker, adding 95% soak with ethanol spends the night, upper strata liquid inclines, be loaded in the chromatographic column of band core, use 200ml ethanol, by resin bed, be washed till effluent and add water and be not white in color till the muddiness with the flow velocity of 2BV/H, with pure water with the flow velocity of 2BV/H by resin bed, being washed till does not have the alcohol flavor.
Get sample on the 10ml70% alcohol reflux liquid, water 70ml is with the flow velocity eluting pillar of 2ml/5min, with 10% ethanol, 20% ethanol, 30% ethanol, 70% ethanol gradient elution.
The rhodioside assay is measured according to high performance liquid chromatography (appendix VI D) in the eluent:
Chromatographic condition: methanol-water (15: 85) is a mobile phase, and the detection wavelength is 275nm, and column temperature is 40 ℃, and flow velocity is 0.6ml/min, and chromatographic column is Akasil C18.
The preparation of reference substance solution: precision takes by weighing the big glycosides reference substance of red scape, adds methanol and makes the solution that every 1ml contains 0.2mg, promptly.
The preparation of need testing solution: will be centrifugal through the water of D-101 macroporous resin and different concentration ethanol eluent (12000r/min, 5min), promptly.
Algoscopy: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject liquid chromatograph, measure, promptly.
The results are shown in Table one.
Table one: rhodioside content in the eluent
70% ethanol elution is not having corresponding peak with the corresponding position of reference substance.
As shown in Table 1, obtain most of rhodioside crude product at 10% ethanol elution place.Paste-forming rate through the dry thing of the eluent of D-101 macroporous resin 10% ethanol elution is 8.64%, and the purity of rhodioside reaches 36.24%.
The ethanol elution consumption is investigated:
Take by weighing Radix Rhodiolae coarse powder 20g, add 70% ethanol, pressed solid-liquid ratio 8: 1,85 ℃ of water-bath reflux, extract, 3 times, each 1h, filter paper filtering, merging filtrate reclaims ethanol to not having alcohol flavor, extracting liquid volume 90ml.
Getting the D-101 macroporous resin puts in the beaker, adding 95% soak with ethanol spends the night, upper strata liquid inclines, be loaded in the chromatographic column of band core, use 200ml ethanol, by resin bed, be washed till effluent and add water and be not white in color till the muddiness with the flow velocity of 2BV/H, with pure water with the flow velocity of 2BV/H by resin bed, being washed till does not have the alcohol flavor.
Get sample on the 10ml70% alcohol reflux liquid, first water 66ml eluting pillar is used 100ml, 150ml, 200ml10% ethanol elution then respectively.。
Table two: the content of rhodioside in 9 eluents
As shown in Table 2, when the water of the column volume that doubles, it is many more to add the rhodioside that 10% ethanol elution of three times of column volumes obtains, eluting complete more.
(2) extraction and purification process of salvianolic acid B
Radix Salviae Miltiorrhizae adds 15 times of amount 50% ethanol, and percolation extracts, and after extracting solution reclaims ethanol, transfers pH2.8.Concentrated solution equal volume of ethyl acetate 4 times, extract merges, and reclaims ethyl acetate, gets salvianolic acid B extractum.Extractum is with 16 times of water dissolutioies to medical material, with the flow velocity of 0.3BV/h, last 8 times to the D101 of medical material macroporous adsorptive resins, static absorption 2h.The composition that is not adsorbed in the sample introduction liquid is removed in washing, and 40% ethanol is with the flow velocity eluting of 0.3BV/h, and concentrating under reduced pressure is removed ethanol, and drying gets salvianolic acid B purification thing.
Research contents:
Extracting method:
Four kinds of method extraction salvianolic acid B extraction ratio measurement results are as follows
Four kinds of methods of table three are extracted the salvianolic acid B extraction ratio relatively
Experimental result shows: the percolation extraction effect is best, and average extraction ratio is more than 20%.
The purification by macroporous resin research of salvianolic acid B is the same.
(3) preparation process
The kind of dropping pill formulation mesostroma, coolant, the best proportioning of medicine and substrate and medicine liquid droplet system temperature.
| PEG4000∶PEG6000 | Medicinal substances extract: substrate | Temperature (℃) | The coolant kind |
| 1.5∶0.5 | 1∶2 | 80 | Liquid paraffin |
Method for making is as follows: get salvianolic acid B purification thing, rhodioside purification thing, Folium Ginkgo extract and prescription other medicines and appropriate amount of PEG 4000: the PEG6000 suitable proportion, and in the Hybrid Heating fusion, 80 ℃ of insulations, splash in the coolant fluid paraffin body, condensation separates, promptly.
Claims (3)
1. a pharmaceutical formulation and preparation thereof of preventing and treating altitude sickness and coronary heart disease, be a kind of medicine of preventing and treating altitude sickness that contains Radix Rhodiolae extract, it is characterized in that: the prescription of this medicine is Radix Rhodiolae 10～40g, Radix Salviae Miltiorrhizae 60～150g, Folium Ginkgo extract 1～5g, artificial Moschus's (perhaps Borneolum Syntheticum) 0.3～1.5g.
2. a kind of pharmaceutical preparation that prevents and treats altitude sickness and coronary heart disease according to claim 1, it is characterized in that: its preparation comprises oral solid formulations such as drop pill, tablet, capsule, granule, pill, powder.
3. drop pill according to claim 2 is characterized in that:
3.1 in the extraction of Radix Salviae Miltiorrhizae extract salvianolic acid B, the method that adopts 10～80% ethanol extractions and ethyl acetate extraction to combine, the used method of purification is a macroporous adsorbent resin, eluent is 20～60% ethanol.
3.2 30～90% ethanol are adopted in the extraction of Radix Rhodiolae extract rhodioside, heating and refluxing extraction, and the used method of purification is a macroporous adsorbent resin, eluent is 10～50% ethanol.
3.3 formulation preparation method is: the PEG4000 that gets Radix Salviae Miltiorrhizae purification thing (salvianolic acid B), Radix Rhodiolae purification thing (high-purity salidroside), Folium Ginkgo extract, artificial Moschus's (Borneolum Syntheticum) and 0.5～3 times of amount by formula proportion: PEG6000 (1～1.5: 0.5～1), mix, heating and melting, 60-80 ℃ of insulation, splash in the coolant condensation, screening, drying, promptly.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104489651A (en) * | 2014-11-27 | 2015-04-08 | 杨宪铭 | Health food taking rhodiola rosea, gingko leaves and American ginseng as active components |
| CN105832860A (en) * | 2016-05-04 | 2016-08-10 | 昆明理工大学 | Fulvic acid oral liquid capable of resisting altitude stress and preparation method thereof |
| CN105920079A (en) * | 2016-06-14 | 2016-09-07 | 山东宏济堂制药集团股份有限公司 | Traditional Chinese medicine composition for treating apoplexy and coronary heart disease and application thereof |
| CN108403858A (en) * | 2018-05-31 | 2018-08-17 | 江西普正制药有限公司 | A kind of beautyberry extract composition that treating hand-foot-and-mouth disease and its application |
| CN108524685A (en) * | 2018-07-03 | 2018-09-14 | 江西普正制药有限公司 | A kind of composition that treating children's herpangina and its application |
| CN109091475A (en) * | 2018-07-06 | 2018-12-28 | 公安部昆明警犬基地 | Working dog altitude sickness prevention pharmaceutical composition and the preparation method and application thereof |
| PL422894A1 (en) * | 2017-09-16 | 2019-03-25 | Szkoła Główna Gospodarstwa Wiejskiego w Warszawie | Method for producing natural herbal preparation and the herbal preparation |
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2009
- 2009-08-28 CN CN2009101728392A patent/CN102000147A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104489651A (en) * | 2014-11-27 | 2015-04-08 | 杨宪铭 | Health food taking rhodiola rosea, gingko leaves and American ginseng as active components |
| CN105832860A (en) * | 2016-05-04 | 2016-08-10 | 昆明理工大学 | Fulvic acid oral liquid capable of resisting altitude stress and preparation method thereof |
| CN105920079A (en) * | 2016-06-14 | 2016-09-07 | 山东宏济堂制药集团股份有限公司 | Traditional Chinese medicine composition for treating apoplexy and coronary heart disease and application thereof |
| PL422894A1 (en) * | 2017-09-16 | 2019-03-25 | Szkoła Główna Gospodarstwa Wiejskiego w Warszawie | Method for producing natural herbal preparation and the herbal preparation |
| PL238576B1 (en) * | 2017-09-16 | 2021-09-06 | Szkola Glowna Gospodarstwa Wiejskiego W Warszawie | Method for producing natural herbal preparation and the herbal preparation |
| CN108403858A (en) * | 2018-05-31 | 2018-08-17 | 江西普正制药有限公司 | A kind of beautyberry extract composition that treating hand-foot-and-mouth disease and its application |
| CN108403858B (en) * | 2018-05-31 | 2020-04-17 | 江西普正制药股份有限公司 | Callicarpa nudiflora extract composition for treating hand-foot-and-mouth disease and application thereof |
| CN108524685A (en) * | 2018-07-03 | 2018-09-14 | 江西普正制药有限公司 | A kind of composition that treating children's herpangina and its application |
| CN108524685B (en) * | 2018-07-03 | 2020-04-17 | 江西普正制药股份有限公司 | Composition for treating children herpangina and application thereof |
| CN109091475A (en) * | 2018-07-06 | 2018-12-28 | 公安部昆明警犬基地 | Working dog altitude sickness prevention pharmaceutical composition and the preparation method and application thereof |
| CN109091475B (en) * | 2018-07-06 | 2021-01-05 | 公安部昆明警犬基地 | Altitude stress resistant pharmaceutical composition for working dogs as well as preparation method and application thereof |
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Open date: 20110406 |