CN101993402A - Natural zeaxanthin ingredient prepared through microbial extraction and preparation method thereof - Google Patents
Natural zeaxanthin ingredient prepared through microbial extraction and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a natural zeaxanthin ingredient prepared through microbial extraction and a preparation method thereof, wherein the ingredient is an extract of marine bacterias, and the extract contains an effective dose of zeaxanthin, is selected from OlleyamarilimosaVIG2317 (strain No: CCTCCM2010201) of flavobacteriaceae and mutant strains thereof, and can be used for preventing or treating skin mutations or human diseases; meanwhile, the extract also has the function of restraining the generation of melanin. The preparation method comprises the following steps: culturing marine bacterias OlleyamarilimosaVIG2317 selected from the flavobacteriaceae in a liquid nutrient medium so as to make pigments containing zeaxanthin; B, separating the marine bacterias from the liquid nutrient medium, then collecting the bacterias so as to obtain the ingredient above; and C, selecting appropriate carriers, then adding the carriers into the collected bacterias. The ingredient provided in the invention can be applied to the fields of health products, food additives, cosmetics, drugs, and the like, and can be used partially and orally.
Description
Technical field
The invention belongs to fields such as heath food, food additive, cosmetics and medicine, relate to the ocean bacterium that a kind of collection is taken from Flavobacterium section (Flavobacteriaceae), be specifically related to a kind of extraction from the Olleya of Flavobacterium section marilimosa, the extraction of more specifically saying so is CCTCC M 2010201 from Olleya marilimosa VIG2317(bacterium numbering) and mutant strain, wherein this extract comprises a kind of zeaxanthin (zeaxanthin) of effective dose, can be used for prevention or treatment human diseases or skin variation.
Background technology
Carotenoid is the fat-soluble pigment of a group, is present in plant, algae and the mushroom.These pigments have been represented distinct colors, for example: red, yellow, tangerine look etc.There are 600 kinds of carotenoid of surpassing to be identified out at present, can be divided into carotene group (carotenes) and xenthophylls group (xanthophylls) according to chemical structure.Xenthophylls group's chemical structure is " containing oxygen carotenoid "; its member comprises xenthophylls (lutein), zeaxanthin, xantheine (antheraxanthin), neoxanthin (neoxanthin) etc.; be present in the food important in the daily life (for example: vegetables, fruit) in order to carry out photoprotection, to avoid over-drastic light intensity destruction vegetable cell.The xenthophylls group also is breezy relevant with human beings'health, and for example: (1) safeguards vision health; (2) risk of reduction cancer; (3) risk of reduction cardiovascular disease.
Zeaxanthin is xenthophylls group's member, is present in the macular area (macular area) of human eye retina jointly with xenthophylls.Since can not make zeaxanthin and xenthophylls in the animal body, therefore must be by absorbing in the food.Because the market requirement, the source of zeaxanthin can be via (1) chemosynthesis (U.S. Pat. No.4,952,716 at present; U.S. Pat. No.5,227,507); (2) plant extraction (U.S. Pat. No.5,648,564; U.S. Pat. No.6,784,351; U.S. Pat. No.6,191,293; U.S. Pat. No.7,150,890; U.S. Pat. No.7,173,145); (3) microorganism extraction (U.S. Pat. No.3,891,504; U.S. Pat. No.3,951,742; U.S. Pat. No.3,951,743; U.S. Pat. No.5,308,759; U.S. Pat. No.5427783).
Because the cost of the zeaxanthin of chemosynthesis is higher, and the human consumer still has security concerns when using this class " artificial products ", therefore has natural product only and just can satisfy the demands of consumers.The source of natural product can be plant or microorganism.Yet with the plant is that natural origin still has its shortcoming, and for example the variation of home environment (weather, water quality) can cause plant biomass to reduce and the difference of contained effective ingredient.Plant biomass reduces can influence the price of buying, thereby causes extracting the wayward of production cost.With the microorganism is that natural origin does not then have above-mentioned shortcoming.In the microorganism extraction process, preponderate, because bacterial classification has fixing and short generation time (generation time), is easier to grow and can lives in simple and cheap advantages such as substratum with strain fermentation.Though the bacterial classification extraction has above-mentioned advantage, yet in order to meet the need of market the purity of present bacterial classification extract and necessity that output all has improvement.
Summary of the invention
For this reason, the present invention is directed to the demand, a kind of novel bacterial that can make zeaxanthin is provided, it is selected from the ocean bacterium of Flavobacterium section (Flavobacteriaceae), specifically be selected from the Olleya marilimosa of Flavobacterium section, more specifically say so be selected from Olleya marilimosa VIG2317(bacterial classification deposit be numbered CCTCC M 2010201) and mutant strain solve the problems referred to above.
The invention provides a kind of microorganism extraction natural corn flavine composition and preparation method thereof, wherein:
A kind of composition is provided, and it is the extract of ocean bacterium, and wherein this extract comprises a kind of zeaxanthin (zeaxanthin) of effective dose, can be used for prevention or treatment human diseases or skin variation.
A kind of new ocean bacterium for preparing above-mentioned composition is provided, and it is to be selected from Flavobacterium section (Flavobacteriaceae) and mutant strain thereof.
A kind of new ocean bacterium for preparing above-mentioned composition is provided, and it is Olleya marilimosa and the mutant strain thereof that is selected from Flavobacterium section.
A kind of new ocean bacterium for preparing above-mentioned composition is provided, and it is that Flavobacterium section Olleya marilimosa VIG2317(bacterium numbering is CCTCC M 2010201) and mutant strain.
A kind of method for preparing above-mentioned composition is provided, comprises: cultivate the Olleya marilimosa VIG2317 that is selected from Flavobacterium section in (1) liquid medium within, contain the pigment of zeaxanthin with manufacturing; (2) thalline is separated with substratum, collect thalline so that obtain above-mentioned composition; (3) select suitable carrier to add the thalline of collecting.
Provide a kind of extra continuity the above-mentioned method for preparing composition, comprising: (4) are pulverized and are collected thalline; (5) decomposition or digestion somatic cells fragment utilize the solvent collection pigment composition that is fit to; (6) remove solvent to collect zeaxanthin.
A kind of composition is provided, and it is the extract that comprises Olleya marilimosa VIG2317 ocean bacterium, and this extract comprises a kind of 0.1～10 milligram (effective dose of 0.1～10mg) zeaxanthin (zeaxanthin).
The extract of a kind of Olleya marilimosa VIG2317 ocean bacterium is provided, and wherein every gram dry cell weight contains the zeaxanthin of about 25 milligrams (25mg).
The extract of a kind of Olleya marilimosa VIG2317 ocean bacterium is provided, and the zeaxanthin of this extract (zeaxanthin) purity is 90%(high-performance liquid chromatograph (HPLC) area percentage at least).
A kind of composition is provided, and it is the extract that comprises Olleya marilimosa VIG2317 ocean bacterium, and this extract has anti-ultraviolet function.
A kind of composition is provided, and it is the extract that comprises Olleya marilimosa VIG2317 ocean bacterium, and this extract has oxidation resistant function.
A kind of composition is provided, and it is the extract that comprises Olleya marilimosa VIG2317 ocean bacterium, and this extract has the outgrowth function of anticancer.In one embodiment, cancer cells can be prostate cancer (prostate carcinoma), colorectal carcinoma (colorectal adenocarcinoma), cancer of the stomach (gastric adenocarcinoma), breast cancer (breast adenocarcinoma), ovarian cancer (ovarian cancer), pharynx cancer (pharynx squamous cell carcinoma), cancer of pancreas (pancreatic adenocarcinoma), choriocarcinoma (choriocarcinoma), bladder cancer (bladder primary carcinoma), thyroid carcinoma (thyroid squamous cell carcinoma).
A kind of composition is provided, and it is the extract that comprises Olleya marilimosa VIG2317 ocean bacterium, and this extract has the function that suppresses the melanochrome generation.
Composition provided by the invention can add suitable carrier or vehicle, and it is to include but not limited to water, oil, organic solvent, can local mode or oral way use.
Composition provided by the invention can be used for fields such as heath food, food additive, cosmetics and medicine.
Technical solution of the present invention is as follows:
A kind of microorganism extraction natural corn flavine composition, it is the extract of ocean bacterium, described extract comprises a kind of zeaxanthin (zeaxanthin) of effective dose, its be the Olleya marilimosa VIG2317(bacterial classification that is selected from Flavobacterium section (Flavobacteriaceae) deposit be numbered CCTCC M 2010201) and mutant strain, can be used for prevention or variation of treatment skin or human diseases.
The zeaxanthin of described effective dose is 0.1～10 milligram (mg).
The every gram dry cell weight of described extract contains the zeaxanthin of about 25 milligrams (25mg).
The zeaxanthin purity that described extract comprises is the 90%(HPLC area percentage at least).
A kind of preparation method of microorganism extraction natural corn flavine composition may further comprise the steps:
Cultivate the ocean bacterium Olleya marilimosa VIG2317 that is selected from Flavobacterium section in A, the liquid medium within, contain the pigment of zeaxanthin with manufacturing;
B, thalline is separated with substratum, collect thalline so that obtain above-mentioned composition;
C, the carrier of selecting to be fit to add the thalline of collecting.
The preparation method of described microorganism extraction natural corn flavine composition is further comprising the steps of:
The thalline that D, pulverizing are collected;
E, decomposition or digestion somatic cells fragment utilize the solvent collection pigment composition that is fit to;
F, removal solvent are to collect zeaxanthin.
Described carrier is an oil.
Described oil comprises and is selected from Semen Maydis oil, fish oil, sweet oil, rapeseed oil, plam oil, Trisun Oil R 80.
Described carrier is a solvent.
Described solvent means acetone, methyl alcohol, ethanol, ethyl acetate, Virahol, hexanaphthene.
A kind of microorganism extraction natural corn flavine composition of the present invention can be used for prevention or treatment human diseases or skin variation.By preparation method of the present invention, can make the pigment that contains zeaxanthin, obtain the composition of zeaxanthin, the carrier of selecting to be fit to adds the thalline of collecting, can also pulverize the collection thalline, decomposition or digestion somatic cells fragment are removed solvent to collect zeaxanthin to utilize suitable solvent collection pigment composition.
A kind of microorganism extraction natural corn flavine composition of the present invention comprises the extract of Olleya marilimosa VIG2317 ocean bacterium, and this extract has the function that suppresses the melanochrome generation, can be used for prevention or variation of treatment skin or human diseases.So-called skin variation is skin aging or cutaneous pigmentation.So-called skin aging is to lead because of in uviolizing.So-called cutaneous pigmentation is to lead because of increasing in melanin content.So-called human diseases is to comprise disease of eye, cardiovascular disease, cancer etc.So-called disease of eye is to lead because of containing quantity not sufficient in macula retinae district zeaxanthin or the serum zeaxanthin contains quantity not sufficient.So-called cardiovascular disease is to lead because of in increasing lipid peroxidation or increase mda content.So-called cancer means prostate cancer, colorectal carcinoma, cancer of the stomach, breast cancer, ovarian cancer, pharynx cancer, cancer of pancreas, choriocarcinoma, bladder cancer, thyroid carcinoma.
Composition provided by the invention can be used for fields such as heath food, food additive, cosmetics and medicine, both can locally use, also can be oral.
Description of drawings
Fig. 1 has shown the yellow bacterium colony of bacterial classification of the present invention (Olley marilimosa VIG2317).
Fig. 2 has represented the purity with HPLC methods analyst bacterial classification zeaxanthin of the present invention extract.
Fig. 3 has shown the photoprotection measurement result of bacterial classification extract of the present invention.
Fig. 4 has shown the antioxygenation measurement result with Ferric thiocyanate methods analyst bacterial classification extract of the present invention.
Fig. 5 has shown the antioxygenation measurement result with Conjugated diene method methods analyst bacterial classification extract of the present invention.
Fig. 6 has shown the antioxygenation measurement result with Liposome-TBARS methods analyst bacterial classification extract of the present invention.
Fig. 7 has shown the antioxygenation measurement result with TEAC methods analyst bacterial classification extract of the present invention.
Fig. 8 has shown the removing free radical ability measurement result with DPPH free radical scavenging methods analyst bacterial classification extract of the present invention.
Fig. 9 has shown the inhibition melanochrome effect measurement result of bacterial classification extract of the present invention.
Figure 10 has shown the anticancer proliferative effect measurement result of bacterial classification extract of the present invention.
Embodiment
1, culture of strains, identify and deposit:
The ocean bacterial strain of obtaining (VIG2317) is coated on the substratum (marine agar) of marine microorganism, temperature was cultivated 2 days for 25 ℃, formed yellow bacterium colony (see figure 1).This bacterium identifies very near Olley marilimosa, 99% similarity is arranged according to 16S rRNA gene order compare of analysis result through Foodstuff Industrial and Development Inst. (FIRDI).Therefore, this bacterial strain called after Olley marilimosa VIG2317, and be deposited at Chinese typical culture collection center on August 11st, 2010, bacterium numbering is CCTCC M 2010201.This bacterial strain (Olley marilimosa VIG2317) does not produce statospore for good gas gram negative bacillus, have the Enzyme of touching, oxidation Enzyme and mobility, its physiology, biochemical characteristic and Olley marilimosa CIP 108537 bacterial strains similar (seeing the following form).
Physiology, the biochemical characteristic comparison sheet of bacterial classification of the present invention (Olley marilimosa VIG2317) and Olley marilimosa CIP 108537 bacterial classifications:
2, high-performance liquid chromatograph (High-performance Liquid Chromatography; HPLC) analyze:
The bacterial classification that fermenter is cultivated is through centrifugal removal supernatant liquor, and the thalline solid substance is with solvent (for example acetone) extraction zeaxanthin.After the lyophilize, the purifying of zeaxanthin is washed with the hexane stream that contains 30% ethyl acetate and is separated through silica gel column chromatography tubing string (silica gel column).After the lyophilize, the purity of zeaxanthin is identified with HPLC.The exsiccant extract is earlier with dissolve with methanol, with 4.6x250mm ODS C-18 column serves as to separate tubing string, injection is 10 μ l, with 20% methanol, 73% acetonitrile, 7% Tris-HCl buffer is towards extract, speed is 1.0 ml/min, at wavelength is to measure under 450 nm, wherein the zeaxanthin purity of this extract 90%(HPLC area percentage at least; See Fig. 2).
3, photoprotection is measured:
The zeaxanthin of extraction with after dimethyl sulfoxide (DMSO) dissolving, is added human fibroblast (CCD-966SK cell strain) respectively with the concentration of 20 μ g/ml, 50 μ g/ml and cultivates.Control group does not then add zeaxanthin.After cultivating 24 hours, with fibroblast irradiation ultraviolet radiation 15 minutes.Through 24 hours cultivation, cell is handled the back with trypsin calculate cell number again with cell counting dish (hemocytometer).Compared to control group, add the fibroblast of the zeaxanthin of 20 μ g/ml and 50 μ g/ml, its cell count increase respectively 18.9% and 33.7%(see Fig. 3).
4, antioxygenation is measured:
A, Ferric thiocyanate method:
The zeaxanthin of extraction is placed on 37 ℃ with 0.2 M potassium phosphate buffer (pH 7.0) dissolving back with linoleic acid emulsion mixing.After 24 hours, get above-mentioned sample mix liquid, add 75% ethanolic soln, 30% ammonium thiocyanate and iron (II) chloride tetrahydrate vibration respectively in regular turn it is mixed.After leaving standstill 1 minute, survey its light absorption value under 500 nm wavelength with minute luminometer.The lower person of light absorption value, promptly inhibiting rate is higher, and the resistance of oxidation of expression specimen is stronger.Present embodiment is also with the xenthophylls (lutein) of same concentrations (60 μ g/ml) and β-Hu Luobusu (β-Carotene) make comparisons, it suppresses, and peroxidation per-cent is respectively 66.9%, 68.6%, 69.7%(sees Fig. 4).The result shows that the prepared zeaxanthin of the present invention is similar to the peroxidation inhibiting rate of xenthophylls and β-Hu Luobusu.
B, Conjugated diene method method:
The zeaxanthin of extraction is placed on 37 ℃ with 0.2 M potassium phosphate buffer (pH 7.0) dissolving back with linoleic acid emulsion mixing.After 15 hours, get above-mentioned sample mix liquid, add 80% methyl alcohol, survey its light absorption value under 234 nm wavelength with minute luminometer.The lower person of light absorption value, promptly inhibiting rate is higher, and the resistance of oxidation of expression specimen is stronger.Present embodiment also will be identical with zeaxanthin concentration (30 μ g/ml) xenthophylls (lutein) and β-Hu Luobusu (β-Carotene) make comparisons, it suppresses, and peroxidation per-cent is respectively 67.4%, 68.7%, 70.3%(sees Fig. 5).The result shows that the prepared zeaxanthin of the present invention is similar to the peroxidation inhibiting rate of xenthophylls and β-Hu Luobusu.
C, Liposome-TBARS method:
The zeaxanthin of extraction is placed on water-bath (37 ℃) with liposome solutions i ron (III) chloride and ascorbic acid mixing after with dissolve with methanol adds butylated hydorxytoluene, thibarbituric acid, trichloroacetic acid after 2 hours.After 20 minutes, above-mentioned sample mix liquid is placed cooled on ice in 100 ℃ of heating in water bath, its light absorption value under 532 nm wavelength is surveyed with minute luminometer in the cooling back.When the light absorption value of 532 nm heal height promptly represent mda (malondialdehyde, MDA) product the more, MDA output is higher, its antioxidant effect is poorer.Present embodiment also is increased to 2000 μ g/ml with the concentration of zeaxanthin by 125 μ g/ml, and the inhibiting rate of MDA then is increased to 80.75%(by 55.23% and sees Fig. 6).The result shows that the prepared zeaxanthin concentration of increase the present invention increases relatively for the inhibition meeting of MDA output.
D, TEAC (trolox equivalent antioxidant capacity) method:
The zeaxanthin of extraction is dissolved back and 2,2'-azino-bis (3-ethylbenz thiazoline-6-sulphonic acid) and potassium persulfate mixing with 0.01 M sodium phosphate buffer.After 30 minutes, above-mentioned sample mix liquid is surveyed its light absorption value under 734 nm wavelength with minute luminometer.When the light absorption value of 734 nm is lower, represent that promptly inhibiting rate is higher, antioxidant effect is better.Present embodiment also is increased to 500 μ g/ml with the concentration of zeaxanthin by 62.5 μ g/ml, and inhibiting rate then is increased to 91.22%(by 15.68% and sees Fig. 7).The result shows, increases the prepared zeaxanthin concentration of the present invention, and its resistance of oxidation can increase relatively.
E, DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging method:
With the zeaxanthin of extraction with dissolve with methanol after be dissolved in 2 of methyl alcohol, 2-diphenyl-1-picrylhydrazyl mixing.After 30 minutes, above-mentioned sample mix liquid is surveyed its light absorption value under 517 nm wavelength with minute luminometer.Represent promptly that when the light absorption value of 517 nm is lower the hydrogen supply capacity of antioxidant is stronger, that is it is stronger to remove the free radical ability.Present embodiment also is increased to 1000 μ g/ml with the concentration of zeaxanthin by 62.5 μ g/ml, and inhibiting rate then is increased to 96.69%(by 10.02% and sees Fig. 8).The result shows, increases the prepared zeaxanthin concentration of the present invention, and it removes the free radical ability can increase relatively.
5, suppressing the melanochrome effect measures:
The zeaxanthin that extracts is added mouse B16 F0 melanoma cell (B16 F0 mouse melanoma cell strain) respectively with the back concentration with 2 μ g/ml, 20 μ g/ml of dimethyl sulfoxide (DMSO) dissolving to be cultivated.Control group does not then add zeaxanthin.After cultivating 24 hours and 48 hours, melanic content is surveyed its light absorption value under 400 nm wavelength with minute luminometer.Present embodiment is increased to 20 μ g/ml with the concentration of zeaxanthin by 2 μ g/ml, and melanic inhibiting rate then is increased to 22.71% by 6.74%.Incubation time was increased to 48 hours by 24 hours, and zeaxanthin concentration 2 μ g/ml then are increased to 32.49% by 6.74% to melanic inhibiting rate; Zeaxanthin concentration 20 μ g/ml then are increased to 36.74%(by 22.71% to melanic inhibiting rate and see Fig. 9).The result shows that increasing the prepared zeaxanthin of the present invention can increase along with the increase of concentration or incubation time respectively melanic inhibiting rate.
6, the anticancer proliferative effect is measured:
The zeaxanthin that extracts is added different human cancer cell's strains respectively with the back concentration with 30 μ g/ml, 60 μ g/ml of dimethyl sulfoxide (DMSO) dissolving to be cultivated 24 hours.Cultivated 4 hours after adding thiazolyl blue tetrazolium bromide, cultivation is removed the back add dimethyl sulfoxide, survey its light absorption value under 590 nm wavelength with minute luminometer again.Present embodiment zeaxanthin concentration is expressed (see figure 10) to suppressing human cancer cell's hyperplasia with cell survival rate (cell viability).Zeaxanthin is when concentration 30 μ g/ml, and human cancer cell's survival rate is respectively prostate cancer 74%, colorectal carcinoma 72.3%, cancer of the stomach 57.3%, breast cancer 78.7%, ovarian cancer 70.3%, pharynx cancer 72.6%, cancer of pancreas 75.6%, choriocarcinoma 72.1%, bladder cancer 45.9%, thyroid carcinoma 70.9%.For non-cancer cells, cell survival rate is respectively retinal pigment epithelium (retinal pigmented epithelium cell; ARPE-19) 105.6%, embryonic fiber parent cell (embryonic fibroblast; BCRC60118) 102.3%, skin fiber parent cell (human skin fibroblast; BCRC 60153) 95.8%.Zeaxanthin is when concentration 60 μ g/ml, and human cancer cell's survival rate is respectively prostate cancer 67.7%, colorectal carcinoma 75.3%, cancer of the stomach 40.1%, breast cancer 61.6%, ovarian cancer 20.3%, pharynx cancer 28.5%, cancer of pancreas 33.4%, choriocarcinoma 40.0%, bladder cancer 16.4%, thyroid carcinoma 71.7%.For non-cancer cells, cell survival rate is respectively retinal pigment epithelium 124.2%, embryonic fiber parent cell 101.2%, skin fiber parent cell 99.8%.The result shows, increasing the prepared zeaxanthin of the present invention does not have inhibition for the hyperplasia of non-cancer cells, then has in various degree inhibition for the hyperplasia of cancer cells.
Below prescription only is some embodiment of the present invention, is not to be used for limiting scope of the invention process.
Prescription one: essence (Essence)
Zeaxanthin (400ppm in butylene glycol) and sodium metabisulfite, ethylhexylglycerin, phenoxyethanol, arginine, dipotassium glycyrrhizate, sodium hyaluronate, PEG-16 macadamia glycerides, acrylates/C10-30 alkyl acrylate crosspolymer, disodium EDTA and water mixing with extraction.
Prescription two: emulsion (Milk Lotion)
Zeaxanthin (400ppm in butylene glycol) and sodium metabisulfite with extraction, citric acid, sodium citrate, tocopheryl acetate, cellulose gum, potassium sorbare, ethylhexylglycerin, bisabolol, ammonium acryloyldi methyltaurate/vp copolymer, butyrospermum parkii (shea butter), beheneth-25, cetyl alcohol, glycerin, cetearyl olivate, isononyl isononanoate, helianthus annuus (sunflower) seed oil, phenoxyethanol, sodium hyaluronate, disodium EDTA and water mixing.
Prescription three: food complementary goods/medicine (Dietary Supplements/ Pharmaceutical Dosage)
The zeaxanthin 10 milligrams (10mg) of extraction is filled in (soft gelatin capsule) in the soft capsule with the Semen Maydis oil dissolving.
Prescription four: food complementary goods/medicine (Dietary Supplements/ Pharmaceutical Dosage)
The zeaxanthin 10 milligrams (10mg) of extraction is added binding to form pill or tablet (tablet).
Prescription five: food complementary goods
The zeaxanthin 10 milligrams (10mg) of extraction is added xenthophylls and other nutrition and antioxidant, be filled in the soft capsule with Semen Maydis oil or peanut oil dissolving.
Prescription six: food complementary goods
The zeaxanthin 10 milligrams (10mg) of extraction is added xenthophylls and other nutrition and antioxidant and/or mineral substance with formation pill or tablet.
In sum, a kind of microorganism extraction natural corn flavine composition of the present invention can be used for prevention or treatment human diseases or skin variation.By preparation method of the present invention, can make the pigment that contains zeaxanthin, obtain the composition of zeaxanthin, the carrier of selecting to be fit to adds the thalline of collecting, can also pulverize the collection thalline, decomposition or digestion somatic cells fragment are removed solvent to collect zeaxanthin to utilize suitable solvent collection pigment composition.A kind of microorganism extraction natural corn flavine composition of the present invention comprises the extract of Olleya marilimosa VIG2317 ocean bacterium, and this extract has the function that suppresses the melanochrome generation.Composition provided by the invention can be used for fields such as heath food, food additive, cosmetics and medicine.
Certainly, those skilled in the art in the present technique field will be appreciated that, the foregoing description only is to be used for illustrating the present invention, and be not as limitation of the invention, as long as in connotation scope of the present invention, all will drop in the scope of claim of the present invention the variation of the foregoing description, modification etc.
Claims (10)
1. a microorganism extracts natural corn flavine composition, it is the extract of ocean bacterium, it is characterized in that, described extract comprises a kind of zeaxanthin (zeaxanthin) of effective dose, its be the Olleya marilimosa VIG2317(bacterial classification that is selected from Flavobacterium section (Flavobacteriaceae) deposit be numbered CCTCC M 2010201) and mutant strain, can be used for prevention or variation of treatment skin or human diseases.
2. microorganism extraction natural corn flavine composition according to claim 1 is characterized in that the zeaxanthin of described effective dose is 0.1～10 milligram (mg).
3. microorganism extraction natural corn flavine composition according to claim 1 is characterized in that the every gram dry cell weight of described extract contains the zeaxanthin of about 25 milligrams (25mg).
4. microorganism extraction natural corn flavine composition according to claim 1 is characterized in that the zeaxanthin purity that described extract comprises is the 90%(HPLC area percentage at least).
5. the preparation method of a microorganism extraction natural corn flavine composition is characterized in that, may further comprise the steps:
Cultivate the ocean bacterium Olleya marilimosa VIG2317 that is selected from Flavobacterium section in A, the liquid medium within, contain the pigment of zeaxanthin with manufacturing;
B, thalline is separated with substratum, collect thalline so that obtain above-mentioned composition;
C, the carrier of selecting to be fit to add the thalline of collecting.
6. the preparation method of microorganism extraction natural corn flavine composition according to claim 5 is characterized in that, and is further comprising the steps of:
The thalline that D, pulverizing are collected;
E, decomposition or digestion somatic cells fragment utilize the solvent collection pigment composition that is fit to;
F, removal solvent are to collect zeaxanthin.
7. the preparation method of microorganism extraction natural corn flavine composition according to claim 5 is characterized in that described carrier is an oil.
8. the preparation method of microorganism according to claim 7 extraction natural corn flavine composition is characterized in that, described oil comprises and is selected from Semen Maydis oil, fish oil, sweet oil, rapeseed oil, plam oil, Trisun Oil R 80.
9. the preparation method of microorganism extraction natural corn flavine composition according to claim 5 is characterized in that described carrier is a solvent.
10. the preparation method of microorganism extraction natural corn flavine composition according to claim 9 is characterized in that described solvent means acetone, methyl alcohol, ethanol, ethyl acetate, Virahol, hexanaphthene.
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| TWI424872B (en) * | 2011-04-25 | 2014-02-01 | Nat Univ Chung Hsing | Extraction of zeaxanthin from |
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| ASKER 等: "Mesoflavibacter zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae", 《SYSTEMATIC AND APPLIED MICROBIOLOGY, URBAN UND FISCHER VERLAG》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI424872B (en) * | 2011-04-25 | 2014-02-01 | Nat Univ Chung Hsing | Extraction of zeaxanthin from |
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