CN101993152B - Activating agent for enhancing degradability of oil degrading bacteria as well as application and use method thereof - Google Patents

Activating agent for enhancing degradability of oil degrading bacteria as well as application and use method thereof Download PDF

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Publication number
CN101993152B
CN101993152B CN2010102886945A CN201010288694A CN101993152B CN 101993152 B CN101993152 B CN 101993152B CN 2010102886945 A CN2010102886945 A CN 2010102886945A CN 201010288694 A CN201010288694 A CN 201010288694A CN 101993152 B CN101993152 B CN 101993152B
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grease
activator
vitamin
mikrobe
activating agent
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CN2010102886945A
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CN101993152A (en
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陈英旭
梁志伟
刘国防
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Zhejiang University ZJU
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Zhejiang University ZJU
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Biological Wastes In General (AREA)

Abstract

The invention discloses an activating agent for enhancing degradability of oil degrading bacteria as well as application and a use method thereof. The activating agent comprises a microorganism excitant, a vitamin solution and a trace element solution in the proportion of 1:1:1, wherein the three solutions are independently preserved before usage, the microorganism excitant contains 1.0-10.0mL/L of Tween80, 0.02-1.0g/L of KH2PO4, 0.01-0.5g/L of MgSO4.7H20, 0.15-4.5g/L of K2HPO4 and 0.5-20.0g/L of cane sugar, the vitamin solution contains 0.5-1.0g/L of vitamin A, 0.5-10.5g/L of vitamin B1, and 0.5-10.0g/L of vitamin C, and the trace element solution contains 0.05-0.15g/L of zinc, 0.02-0.10g/L of cobalt,, 0.15-0.40g/L of manganese, 0.02-0.20g/L of copper, 0.04-0.35g/L of molybdenum, 0.02-0.15g/L of nickel, 0.04-0.30g/L of selenium, and 0.0007-0.003g/L of boron. When the activating agent is used, the microorganism activating agent accounting for 0.03% of the volume of oil sewage is respectively taken and directly added into a sewage treatment system once 7-14 days. The activating agent is used for oil sewage treatment and can enhance the degradability of oil degrading microorganisms.

Description

A kind of activator that strengthens grease degradation bacteria degradation capability and uses thereof and method of use
Technical field
The present invention relates to a kind of activator that strengthens grease degradation bacteria degradation capability and uses thereof and method of use.This activator is by biological exciting agent, and VITAMINs is formed with trace element, can promote microorganism growth, strengthens metabolic activity, improves system stability, reduce processing costs.
Background technology
The grease that contains a large amount of difficult for biological degradation in the sewage from slaughterhouse, milk preparation source mill, catering trade etc.The not treated water body on every side that enters of high density grease sewage can form oil film at the water surface, hinders the oxygen transmission, causes water hypoxia, threatens the existence of aquatic animals and plants.In anaerobic biological treatment, grease can accumulate in Sludge Surface, hinders the transmission of solubility pollutent, reduces the degradation efficiency of mikrobe to pollutent; A large amount of greases adhere in mud, reduce sludge settling property, cause the sedimentation difficulty, cause active sludge to run off in a large number, finally influence the processing efficiency of system, operation problems such as stench, obstruction occur.
Tween-80, K +, Mg 2+Can stimulate grease degraded microbic activity with material such as sucrose, promote the secretion of fat hydrolase, accelerate greasy degradation rate, reduce the influence of grease anaerobic biological treatment.
Adding nutritive elements such as trace element and VITAMINs is one of most effectual way of optimizing microorganism growth environment, raising sewage treating efficiency.The small size change of nutritive element can excite multiple potentiality microbial growth in the growing environment, thereby brings tremendous influence for treatment system.Facts have proved of each field, it is all very essential in scientific research and engineering practice to add nutritive element.
In the grease Sewage treatment systems, the shortage of trace element will cause the increase of voltaile fatty acid, causes pH reduction, BOD increase, scum silica frost accumulation, finally has influence on the processing efficiency of total system.Competent micro-metals and sulfide are the assurances of anaerobism WWT.In the actual motion, when the volatile acid of anaerobism water outlet increases, should consider the shortage of trace element.
The present invention has found a kind of grease is degraded that the nutritional formula of obvious promoter action is arranged biological exciting agent, VITAMINs and micro-optimum combination, through suitable adding, can improve the processing efficiency and the stability of system.
Summary of the invention
The purpose of this invention is to provide a kind of activator that strengthens grease degradation bacteria degradation capability and uses thereof and method of use.
The activator that strengthens grease degradation bacteria degradation capability comprises that volume ratio is 1: 1: 1 mikrobe exciting agent, vitamin solution and a trace element solution, uses first three solution independently to preserve; Wherein, the mikrobe exciting agent comprises Tween801.0~10.0mL/L, KH 2PO 40.02~1.0g/L, MgSO 47H 200.01~0.5g/L, K 2HPO 40.15~4.5g/L and sucrose 0.5~20.0g/L; Vitamin solution comprises vitamin A 0.5~10.0g/L, vitaminB10 .5~10.0g/L, vitamins C 0.5~10.0g/L, and trace element solution comprises zinc 0.05~0.15g/L, cobalt 0.02~0.10g/L, manganese 0.15~0.40g/L, copper 0.02~0.20g/L, molybdenum 0.04~0.35g/L, nickel 0.02~0.15g/L, selenium 0.04~0.30g/L, boron 0.0007~0.003g/L.
Said activator is liquid form.The activator of described enhancing grease degradation bacteria degradation capability is used for the grease WWT, can strengthen the degradation capability of grease degraded mikrobe.The method of use of the activator of described enhancing grease degradation bacteria degradation capability is: get the mikrobe activator of grease sewage volume 0.03% respectively, directly be added to Sewage treatment systems and get final product.The cycle of adding be 7~14 days/inferior.
The present invention is a kind of nutritional formula that promotes microbial metabolism.Under the stimulus effects of this nutritional formula, grease degraded mikrobe degraded grease and meta-bolites thereof more effectively in the sewage; Niche is abundanter, and different types of mikrobe carries out metabolism more efficiently through close cooperation, thereby improves sewage treating efficiency, reduction processing costs.This activator manufacture craft and use-pattern are simple, and high density grease sewage treatment effect is had remarkable castering action, have very high industrial applications and are worth.
Description of drawings
Fig. 1 adds activator front and back up flow anaerobic sludge blanket reactor to the grease degradation effect.
Embodiment
The activator that strengthens grease degradation bacteria degradation capability comprises that volume ratio is 1: 1: 1 mikrobe exciting agent, vitamin solution and a trace element solution, uses first three solution independently to preserve; Wherein, the mikrobe exciting agent comprises Tween801.0~10.0mL/L, KH 2PO 40.02~1.0g/L, MgSO 47H 200.01~0.5g/L, K 2HPO 40.15~4.5g/L and sucrose 0.5~20.0g/L; Vitamin solution comprises vitamin A 0.5~10.0g/L, vitaminB10 .5~10.0g/L, vitamins C 0.5~10.0g/L, and trace element solution comprises zinc 0.05~0.15g/L, cobalt 0.02~0.10g/L, manganese 0.15~0.40g/L, copper 0.02~0.20g/L, molybdenum 0.04~0.35g/L, nickel 0.02~0.15g/L, selenium 0.04~0.30g/L, boron 0.0007~0.003g/L.
Said activator is liquid form.The activator of described enhancing grease degradation bacteria degradation capability is used for the grease WWT, can strengthen the degradation capability of grease degraded mikrobe.The method of use of the activator of described enhancing grease degradation bacteria degradation capability is: get the mikrobe activator of grease sewage volume 0.03% respectively, directly be added to Sewage treatment systems and get final product.The cycle of adding be 7~14 days/inferior.
Embodiment 1: the activator preparation method who strengthens the grease degradation capability
The activator that strengthens grease degradation bacteria degradation capability comprises that volume ratio is 1: 1: 1 mikrobe exciting agent, vitamin solution and a trace element solution.Three kinds of solution compound methods are following: get Tween801.0mL, KH 2PO 40.02g, MgSO 47H 200.01g, K 2HPO 40.15g, sucrose 0.5g and zero(ppm) water 1000mL in the 1L narrow-mouthed bottle, glass stick stirs and makes its dissolving, obtains the mikrobe exciting agent; Get vitamin A 0.5g, vitaminB10 .5g, vitamins C 0.5g and zero(ppm) water 1000mL in the 1L narrow-mouthed bottle, glass stick stirs and makes its dissolving, obtains vitamin solution; Get ZnSO 47H 2O 0.22g, CoCl 26H 2O 0.08g, MnCl 24H 2O 0.54g, CuSO 45H 2O 0.08g, NaMoO 4H 2O 0.08g, NiCl 26H 2O 0.08g, Na 2SeO 30.08g, HBO 40.005g with zero(ppm) water 1000mL in the 1L narrow-mouthed bottle, glass stick stirs and to make its dissolving, obtains trace element solution.Use first three solution independently to preserve.
Embodiment 2: the activator preparation method who strengthens the grease degradation capability
The activator that strengthens grease degradation bacteria degradation capability comprises that volume ratio is 1: 1: 1 mikrobe exciting agent, vitamin solution and a trace element solution.Three kinds of solution compound methods are following: get Tween8010.0mL, KH 2PO 41.0g, MgSO 47H 200.5g, K 2HPO 44.5g, sucrose 20g and zero(ppm) water 1000mL in the 1L narrow-mouthed bottle, glass stick stirs and makes its dissolving, obtains the mikrobe exciting agent; Get vitamin A 10g, vitamins B 110g, vitamins C 10g and zero(ppm) water 1000mL in the 1L narrow-mouthed bottle, glass stick stirs and makes its dissolving, obtains vitamin solution; Get ZnSO 47H 2O 0.66g, CoCl 26H 2O 0.40g, MnCl 24H 2O1.44g, CuSO 45H 2O 0.80g, NaMoO 4H 2O 0.73g, NiCl 26H 2O 0.61g, Na 2SeO 30.66g, HBO 40.02g with zero(ppm) water 1000mL in the 1L narrow-mouthed bottle, glass stick stirs and to make its dissolving, obtains trace element solution.Use first three solution independently to preserve.
Embodiment 3: said activator is added to up flow anaerobic sludge blanket reactor (UASB reactor drum)
Up flow anaerobic sludge blanket reactor of handling grease sewage in the laboratory, useful volume is 18L.According to the dosage of sewage volume 0.03%, get 5.4mL mikrobe activator, be added in the reactor drum.Whenever added once at a distance from 8 days later, dosage is the same.
Embodiment 4: eliminate the scum silica frost experiment
When the flow lifting type anaerobic reactor is handled grease sewage, start back one month and a large amount of scum silica frost occurred., alkali molten through acid dissolves and reaches the surfactant dissolves test, and through high temperature (105 ℃) oven dry, infers that this scum silica frost is the calcium salt or the magnesium salts of longer chain fatty acid.
Mikrobe activator of the present invention is added in the sewage according to dosing method described in the embodiment 3, and after the week, scum silica frost begins fragmentation, and scum silica frost disappears basically after two weeks.
Embodiment 5: add the activator post-reactor grease degradation capability is strengthened experiment
The flow lifting type anaerobic reactor is as shown in Figure 1 to greasy degradation effect.Do not add before the activator, along with the operation of reactor drum, processing efficiency is more and more lower, the effluent quality variation.At the bottom of in November, 2009, water outlet COD almost equates that with water inlet COD have a large amount of scum silica frost in the reactor drum this moment.On December 2nd, 2009, beginning adds the mikrobe activator according to dosing method described in the embodiment 3, begins to reduce significantly after the increase that water outlet COD experience is of short duration subsequently.After one month, water outlet COD stable maintenance is about 700mg/L.

Claims (4)

1. an activator that strengthens grease degradation bacteria degradation capability is characterized in that comprising that volume ratio is 1: 1: 1 mikrobe exciting agent, vitamin solution and a trace element solution, uses first three solution independently to preserve; Wherein, the mikrobe exciting agent comprises Tween80 1.0~10.0mL/L, KH 2PO 40.02~1.0g/L, MgSO 47H 2O 0.01~0.5g/L, K 2HPO 40.15~4.5g/L and sucrose 0.5~20.0g/L; Vitamin solution comprises vitamin A 0.5~10.0g/L, VITMAIN B1 0.5~10.0g/L, vitamins C 0.5~10.0g/L, and trace element solution comprises zinc 0.05~0.15g/L, cobalt 0.02~0.10g/L, manganese 0.15~0.40g/L, copper 0.02~0.20g/L, molybdenum 0.04~0.35g/L, nickel 0.02~0.15g/L, selenium 0.04~0.30g/L, boron 0.0007~0.003g/L.
2. a kind of activator that strengthens grease degradation bacteria degradation capability according to claim 1 is characterized in that said activator is liquid form.
3. a purposes that strengthens the activator of grease degradation bacteria degradation capability according to claim 1 is characterized in that being used for the grease WWT, can strengthen the degradation capability of grease degraded mikrobe.
4. method of use that strengthens the activator of grease degradation bacteria degradation capability according to claim 1; It is characterized in that its dosing method is: during use; The mikrobe activator of getting grease sewage volume 0.03% respectively directly is added to Sewage treatment systems and gets final product, the cycle of adding be 7~14 days/inferior.
CN2010102886945A 2010-09-21 2010-09-21 Activating agent for enhancing degradability of oil degrading bacteria as well as application and use method thereof Expired - Fee Related CN101993152B (en)

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CN105907666A (en) * 2016-04-22 2016-08-31 浙江大学 Preparation method and application of grease degrading bacterium community
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