CN101982049A - Method for identifying fleahopper-resistant cotton - Google Patents
Method for identifying fleahopper-resistant cotton Download PDFInfo
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- CN101982049A CN101982049A CN 201010284395 CN201010284395A CN101982049A CN 101982049 A CN101982049 A CN 101982049A CN 201010284395 CN201010284395 CN 201010284395 CN 201010284395 A CN201010284395 A CN 201010284395A CN 101982049 A CN101982049 A CN 101982049A
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Abstract
The invention relates to a method for identifying fleahopper-resistant cotton. The method has the following steps: a control variety and a checked variety are planted in a screen growth room; the fleahopper are released at the full bud period of cotton growth; harm to blades and squares of the control variety and the checked variety is investigated six days after larvae are inoculated and the cotton variety resistant to the fleahopper is identified according to the blade harm index decreasing rate and the square harm index decreasing rate. The method provided by the invention features good practicability and operability and stable and reliable identification result.
Description
Technical field
The present invention relates to the authentication method of a kind of anti-fleahopper silk worm cotton.
Background technology
1997, China began commercialization plantation transgenic cotton against pests, accounted for about 70% of the total cultivated area of national cotton to the cultivated area of transgenic cotton against pests in 2009.Up to the present the pest-resistant type of domestic Insect Resistant Cotton is single, is lepidoptera pest kinds such as bollworm resisting substantially.At present, extensively the trans Bt gene cotton of plantation has been controlled the harm of main lepidoptera pests such as cotton bollworm effectively, thereby reduced the usage amount of cotton field chemical pesticide, especially the usage amount in the fleahopper silk worm takes place by the key period reduces significantly, thereby cause fleahopper silk worm population quantity to increase severely, make fleahopper silk worm grade want insect to rise to primary pest, and be serious catastrophe trend development.
The fleahopper silk worm is the important pest of a class, and its host plant is in extensive range, reported 50 surplus section's kind more than 200, comprise multiple important crops such as cotton, jujube, grape, cherry, apple, tea tree.In getting the food process, nymph and adult inhale juice in the tender tissue of cotton plant with needle-like mouthpart thorn, and carry out external predigestion from the saliva that salivary gland secretion contains ptyalin.Also have the pathogenic protein that causes cotton pathological characters to occur in addition in fleahopper silk worm saliva, so top in fleahopper silk worm harm cotton, it is withered dead to cause the cotton terminal bud, becomes cotton without a head; The harm cotton is top, causes cotton and forms many indefinite buds, becomes the bull cotton, and the performance branches and leaves are grown thickly; Behind the harm cotton leaf, the part of damage blackening, the back forms 1 aperture, and after blade was grown up, aperture was also grown up thereupon, became a lot of broken holes, and it is crazy to be referred to as brokenly leaf; The killed back of square is black by xanthochromia, and likeness in form buckwheat grain came off after 2-3 days; Children bell killed the lighter presents brown water logging spot, and weight person is abound with that stain is rigid to come off.
The control of present stage to cotton field fleahopper silk worm mainly relies on chemical insecticide, and along with a large amount of uses of insecticide certainly will cause the fleahopper silk worm that insecticide is produced resistance, it is very necessary to seek new fleahopper silk worm control approach.Current scientist does not also excavate and is cloned into the gene of effective control fleahopper silk worm, and the cotton variety of cultivating the anti-fleahopper silk worm of transgenosis also needs the long time.Therefore in existing transgenic pest-resistant cotton and conventional cotton, identify the cotton variety of anti-fleahopper silk worm quickly and accurately, and by molecule labelling method, new material is created in polymerization, offered breeding man and use, become extremely urgent work.Therefore, be badly in need of the authentication method of a kind of anti-fleahopper silk worm cotton.
Summary of the invention
The authentication method that the purpose of this invention is to provide a kind of anti-fleahopper silk worm cotton.
The authentication method of anti-fleahopper silk worm cotton of the present invention comprises check variety and is examined kind and be planted in the solarium, discharge fleahopper silk worm adult in the Sheng flower bud phase of cotton growth, connect investigation in the 6th day behind the worm and respectively examined the harm situation of the blade and the square of kind and check variety, identify the cotton variety of anti-fleahopper silk worm according to go down rate and the killed rate of going down of square of blade hazard index.
In another embodiment, the authentication method of anti-fleahopper silk worm cotton of the present invention comprises the steps:
(1) test is carried out in the solarium, plants check variety respectively and examined kind in the solarium;
(2) be covered with guard in the outside, solarium, to stop the intrusion of insect and natural enemy;
(3) discharge fleahopper silk worm adult in the Sheng flower bud phase of cotton growth, every square metre discharges 2;
(4) discharge preceding 10 days clean cages of fleahopper silk worm, in the solarium, spray insecticide with kill pests and natural enemy;
(5) connect the harm situation that kind and check variety blade and square are respectively examined in investigation in the 6th day behind the worm, record blade hazard level; The blade symptom of being injured is that blade presents uneven hole, brown or black spots occur; Square is injured symptom for the square bract presents black thorn point, serious square turns black; The criterion of blade hazard level: 0 grade is not had the harm shape for blade; Slightly be injured for blade for 1 grade, injured area is lower than 5%; 2 grades are medium blade harm, and injured area is greater than 5%, less than 20%; Seriously be injured for blade for 3 grades, injured area is greater than 20%, less than 50%; 4 grades for blade endangers same check variety, and injured area is greater than 50%.
(6) calculate blade hazard index, blade hazard index go down rate, square rate of damage, the killed rate that goes down of square;
The blade hazard index is the mean value of the hazard level of 5 blades in every strain cotton top;
The blade hazard index rate that goes down is calculated according to formula I:
Formula I:
The square rate of damage calculates according to formula II:
Formula II:
The killed rate that goes down of square is calculated according to formula III:
Publicity III:
(7), identify the cotton variety of anti-fleahopper silk worm according to blade hazard index go down rate and the killed rate that goes down of square.
Authentication method of the present invention has been got rid of the interference of factors such as other insects and natural enemy, has stronger practicality and operability, and qualification result is stable, reliable.Authentication method of the present invention can identify anti-fleahopper silk worm cotton variety quickly and accurately, and the cotton variety that identifies thus can carry out seed selection and improvement reliably, and popularizing planting widely, thereby obtains huge economic benefit.
Embodiment
Embodiment 1:
Test procedure:
(1) test is carried out in long 20 meters, wide 2.5 meters, high 1.8 meters solarium, and the cotton 213gl of this word that provides with The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute germ plasm resource chamber is a check variety, the weighing apparatus that is provided with the dry farming of academy of agricultural sciences, Hebei province cotton No. 4 for being examined kind.Cotton No. 4 each 10 strains of cotton 213gl of this word of plantation and weighing apparatus repeat 3 times in the solarium, plant cotton No. 4 each 30 strains of cotton 213gl of this word and weighing apparatus altogether, and cotton culture line-spacing 0.80m, spacing in the rows 0.25m in the solarium can prevent and treat the seedling aphid seedling stage 1-2 time;
(2) be covered with 80 purpose nylon wire guards in the outside, solarium, to stop the intrusion of insect and natural enemy;
(3) discharge fleahopper silk worm adult in the Sheng flower bud phase of cotton growth, every square metre discharges 2.The fleahopper silk worm that is discharged through the adult of indoor kidney bean artificial feeding, selects the strong adult of mobility to discharge for picking up from field nature population during release;
(4) discharge preceding 10 days clean cages of fleahopper silk worm, in the solarium, spray 1500 times of liquid of phoxim, kill pests and natural enemy;
(5) connect behind the worm blade of investigating cotton No. 4 of the cotton 213gl of this word and weighing apparatus on the 6th day respectively and the harm situation of square, record blade harm progression;
(6) calculate blade hazard index, blade hazard index go down rate, square rate of damage, the killed rate that goes down of square, result of the test is as shown in table 1.
Embodiment 2-7:
Test procedure is with embodiment 1, except the Shanxi cotton 26 that provides with Shanxi Academy of Agricultural Sciences Cotton Research Institute respectively, The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute germ plasm resource chamber provide in 4717, No. 1, BRS-10, Chizhou City red leaf cotton, HG-E-12 and stone subbreed be for being examined kind.Result of the test is also as shown in table 1.
The result of the test of the anti-fleahopper silk worm of table 1. cotton variety
By experimental result as seen, cotton 213gl is contrast with this word, and No. 1 blade hazard index of stone subbreed is 90.97%, and the killed rate of going down of square is 88.51%, and synthetic determination is high anti-; Cotton No. 4 of weighing apparatus, in 4717, Shanxi cotton 26, BRS-10, Chizhou City red leaf cotton, the HG-E-12 blade hazard index rate of going down be respectively: 67.07%, 68.83%, 69.34%, 71.43%, 80.95%, 67.97%, the killed rate of going down of square is respectively: 70.18%, 71.63%, 72.28%, 72.46%, 73.83% and 86.29%, and synthetic determination is anti-level.
Experimental result of the present invention conforms to the actual result of field test.Therefore, identify the stone subbreed and be high anti-fleahopper silk worm cotton material No. 1, can be used for seed selection and the improvement and the popularizing planting of anti-fleahopper silk worm cotton variety.
Claims (2)
1. the authentication method of an anti-fleahopper silk worm cotton, it comprises check variety and is examined kind and be planted in the solarium, discharge fleahopper silk worm adult in the Sheng flower bud phase of cotton growth, connect investigation in the 6th day behind the worm and respectively examined the harm situation of the blade and the square of kind and check variety, identify the cotton variety of anti-fleahopper silk worm according to go down rate and the killed rate of going down of square of blade hazard index.
2. authentication method as claimed in claim 1, it comprises the steps:
(1) in the solarium, plants check variety respectively and examined kind;
(2) be covered with guard in the outside, solarium, to stop the intrusion of insect and natural enemy;
(3) discharge fleahopper silk worm adult in the Sheng flower bud phase of cotton growth, every square metre discharges 2;
(4) discharge preceding 10 days clean cages of fleahopper silk worm, in the solarium, spray insecticide with kill pests and natural enemy;
(5) connect the harm situation that kind and check variety blade and square are respectively examined in investigation in the 6th day behind the worm, record blade hazard level; The blade symptom of being injured is that blade presents uneven hole, brown or black spots occur; Square is injured symptom for the square bract presents black thorn point, serious square turns black; The criterion of blade hazard level: 0 grade is not had the harm shape for blade; Slightly be injured for blade for 1 grade, injured area is lower than 5%; 2 grades are medium blade harm, and injured area is greater than 5%, less than 20%; Seriously be injured for blade for 3 grades, injured area is greater than 20%, less than 50%; 4 grades for blade endangers same check variety, and injured area is greater than 50%;
(6) calculate blade hazard index, blade hazard index go down rate, square rate of damage, the killed rate that goes down of square;
The blade hazard index is the mean value of the hazard level of 5 blades in every strain cotton top;
The blade hazard index rate that goes down is calculated according to formula I:
Formula I:
The square rate of damage calculates according to formula II:
Formula II:
The killed rate that goes down of square is calculated according to formula III:
Publicity III:
(7), identify the cotton variety of anti-fleahopper silk worm according to blade hazard index go down rate and the killed rate that goes down of square.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010284395 CN101982049B (en) | 2010-09-13 | 2010-09-13 | Method for identifying fleahopper-resistant cotton |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010284395 CN101982049B (en) | 2010-09-13 | 2010-09-13 | Method for identifying fleahopper-resistant cotton |
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| CN101982049A true CN101982049A (en) | 2011-03-02 |
| CN101982049B CN101982049B (en) | 2012-10-31 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365314A (en) * | 2014-01-26 | 2015-02-25 | 江西省农业科学院植物保护研究所 | Method for identifying thrip resistance of asparagus |
| CN104719013A (en) * | 2015-03-25 | 2015-06-24 | 中国农业科学院棉花研究所 | Wild cotton or semi-wild cotton mirid resistance identification method |
| CN105191679A (en) * | 2015-06-19 | 2015-12-30 | 广西壮族自治区农业科学院玉米研究所 | Anti-weevil sweet potato identification method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802896A (en) * | 2006-01-05 | 2006-07-19 | 江苏省农业科学院 | Cotton breeding artificial isolation chamber anti- cotton aphid authentication method |
| CN101592646A (en) * | 2008-05-28 | 2009-12-02 | 中国农业科学院植物保护研究所 | The authentication method of fleahopper resistance of cotton |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802896A (en) * | 2006-01-05 | 2006-07-19 | 江苏省农业科学院 | Cotton breeding artificial isolation chamber anti- cotton aphid authentication method |
| CN101592646A (en) * | 2008-05-28 | 2009-12-02 | 中国农业科学院植物保护研究所 | The authentication method of fleahopper resistance of cotton |
Non-Patent Citations (5)
| Title |
|---|
| 《中国棉花》 20070531 雒珺瑜等 2005年黄河流域棉区抗虫棉区试抗棉铃虫性鉴定结果评述 第17-19页 1-2 , 第5期 2 * |
| 《中国棉花》 20091130 雒珺瑜等 2004-2008年黄河流域国家区域试验抗虫鉴定结果评述 第11-20页 1-2 第36卷, 第11期 2 * |
| 《中国棉花学会2009年年会论文汇编》 20091231 何炼等 四川省区试棉花抗病虫性鉴定与评价 第78-80页 1-2 , 2 * |
| 《棉花学报》 19941231 马丽华等 棉花抗蚜性鉴定方法及分级标准修订 第86页 1-2 , 第6期 2 * |
| 《植物保护科技创新与发展--中国植物保护学会2008年学术年会论文集》 20081231 曾华兰等 棉花品种抗病虫性鉴定评价研究 第670-675页 1-2 , 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365314A (en) * | 2014-01-26 | 2015-02-25 | 江西省农业科学院植物保护研究所 | Method for identifying thrip resistance of asparagus |
| CN104719013A (en) * | 2015-03-25 | 2015-06-24 | 中国农业科学院棉花研究所 | Wild cotton or semi-wild cotton mirid resistance identification method |
| CN105191679A (en) * | 2015-06-19 | 2015-12-30 | 广西壮族自治区农业科学院玉米研究所 | Anti-weevil sweet potato identification method |
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| CN101982049B (en) | 2012-10-31 |
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