CN101975851A - Reagent for classifying leukocytes - Google Patents
Reagent for classifying leukocytes Download PDFInfo
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- CN101975851A CN101975851A CN2010102787102A CN201010278710A CN101975851A CN 101975851 A CN101975851 A CN 101975851A CN 2010102787102 A CN2010102787102 A CN 2010102787102A CN 201010278710 A CN201010278710 A CN 201010278710A CN 101975851 A CN101975851 A CN 101975851A
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- reagent
- leucocyte
- agent
- hemolytic agent
- leukocytes
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Abstract
The invention discloses a reagent for classifying leukocytes, which can be used for classifying leukocytes into five groups corresponding to lymphocytes, monocytes, eosinophilic granulocytes, neutrophilic granulocytes and basophilic granulocytes. The reagent comprises a buffering agent and a hemolytic agent, wherein the hemolytic agent comprises at least two types of betaine type amphoteric surfactants. The reagent can be applied in wide pH value and temperature ranges, does not damage the leukocytes and can be used for accurately classifying white blood cells.
Description
Technical field
The invention belongs to the blood cell analysis field, particularly relate to a kind of reagent that leucocyte is classified.
Background technology
People's whole blood sample is carried out the cytological classification counting to have very important significance in clinical diagnosis.The reagent that leucocyte is classified that the present invention relates to is meant five classification agents that leucocyte can be divided into lymphocyte, monocyte, eosinophil, neutrophil cell and basophilic granulocyte.Up to now, existing multiple such reagent is as containing two kinds of cationic surfactants, a kind of organic compound with hydrophobic grouping and anionic group in the prescription; Contain a kind of cationic surfactant and a kind of non-ionic surfactant etc. in the prescription.But there is following problem in these reagent: the first, can only in a narrow and small PH scope, use; The second, the serviceability temperature narrow range; The 3rd destructible leucocyte causes leukocyte differential count inaccurate.
Summary of the invention
The objective of the invention is the shortcoming at above technology, providing a kind of can use in broad pH value and temperature range, and the reagent that does not destroy leucocyte, classifies and accurately leucocyte is classified.
For achieving the above object, the technical solution used in the present invention is: the reagent that leucocyte is classified, comprise buffering agent and hemolytic agent, described hemolytic agent is made up of at least two kinds of betaine type zwitterionic surface-active agents, described betaine type zwitterionic surface-active agent has the structure shown in the formula I
R represents alkyl or contains the alkyl group of 6～20 carbon atoms among the formula I.
The content range of hemolytic agent is 2～15g/L.
In the technical scheme of the present invention, hemolytic agent is made up of at least two kinds of betaine type zwitterionic surface-active agents, preferred empgen BB, hexadecyldimethyl benzyl ammonium betaine.Select the betaine type zwitterionic surface-active agent for use, do not destroy leucocyte.Buffering agent can be selected from N-(2-acetamide) iminodiacetic acid, trishydroxymethylaminomethane hydrochloric acid etc.
Utilize reagent of the present invention to carry out leukocytic classification and Detection, red blood cell can be dissolved and accurately leucocyte is divided into lymphocyte, monocyte, eosinophil, neutrophil cell and basophilic granulocyte rapidly.This reagent can use in broad PH scope, and pH value 1～10 the scope of being controlled at gets final product, and this controls by buffering agent.This temperature of reagent usable range is wide, can use in 2～40 ℃ temperature range.
Overcome the shortcoming of prior art from the top narration reagent that leucocyte is classified of the present invention as can be known, had and in broad pH value and temperature range, to use, and do not destroy leucocyte, the advantage accurately of classifying.
Description of drawings
Fig. 1 is a betaine type zwitterionic surface-active agent of the present invention when being made up of empgen BB and hexadecyldimethyl benzyl ammonium betaine, the figure as a result when the present invention is used for leukocyte differential count and detects;
Embodiment
Embodiment 1
To the reagent that leucocyte is classified, comprise buffering agent and hemolytic agent, described hemolytic agent is made up of two kinds of betaine type zwitterionic surface-active agents, is respectively empgen BB and hexadecyldimethyl benzyl ammonium betaine.Described betaine type zwitterionic surface-active agent has the structure shown in the formula I, and the content range of hemolytic agent is 10.5g/L.Buffering agent is N-(2-acetamide) iminodiacetic acid., as detecting instrument fresh and healthy people whole blood sample is joined in the cellanalyzer with Japanese photoelectricity MEK7222K type cellanalyzer, pH value is controlled at 1～10 scope, and temperature is to detect under 8 ℃ the condition, and testing result as shown in Figure 1.
Embodiment 1 can accurately be divided into lymphocyte, monocyte, eosinophil, neutrophil cell and basophilic granulocyte with leucocyte as can be seen from Figure 1.
Claims (3)
1. the reagent that leucocyte is classified is characterized in that comprising buffering agent and hemolytic agent, and described hemolytic agent is made up of at least two kinds of betaine type zwitterionic surface-active agents.
2. the reagent that leucocyte is classified according to claim 1 is characterized in that described betaine type zwitterionic surface-active agent has the structure shown in the formula I,
R represents alkyl or contains the alkyl group of 6～20 carbon atoms among the formula I.
3. the reagent that leucocyte is classified according to claim 1 and 2, the content range that it is characterized in that described hemolytic agent is 2～15g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102787102A CN101975851A (en) | 2010-09-13 | 2010-09-13 | Reagent for classifying leukocytes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102787102A CN101975851A (en) | 2010-09-13 | 2010-09-13 | Reagent for classifying leukocytes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101975851A true CN101975851A (en) | 2011-02-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102787102A Pending CN101975851A (en) | 2010-09-13 | 2010-09-13 | Reagent for classifying leukocytes |
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| CN (1) | CN101975851A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014015687A1 (en) * | 2012-07-27 | 2014-01-30 | 深圳迈瑞生物医疗电子股份有限公司 | White blood cell classifying and counting agent and white blood cell classifying method |
| CN108414427A (en) * | 2017-02-10 | 2018-08-17 | 深圳市帝迈生物技术有限公司 | A kind of leucocyte classification reagent |
| CN110132908A (en) * | 2018-02-09 | 2019-08-16 | 深圳市帝迈生物技术有限公司 | A kind of cell detection kit and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127886A (en) * | 1993-12-22 | 1996-07-31 | 东亚医用电子株式会社 | Reagent for analysing leucocyte |
| CN1814691A (en) * | 2005-02-03 | 2006-08-09 | 刘春德 | Surfactant formula system compounded by ampho-surfactant and use in the third oil production |
-
2010
- 2010-09-13 CN CN2010102787102A patent/CN101975851A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1127886A (en) * | 1993-12-22 | 1996-07-31 | 东亚医用电子株式会社 | Reagent for analysing leucocyte |
| CN1814691A (en) * | 2005-02-03 | 2006-08-09 | 刘春德 | Surfactant formula system compounded by ampho-surfactant and use in the third oil production |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014015687A1 (en) * | 2012-07-27 | 2014-01-30 | 深圳迈瑞生物医疗电子股份有限公司 | White blood cell classifying and counting agent and white blood cell classifying method |
| CN103575702A (en) * | 2012-07-27 | 2014-02-12 | 深圳迈瑞生物医疗电子股份有限公司 | Leucocyte differential count reagent and leucocyte classification method |
| CN103575702B (en) * | 2012-07-27 | 2016-06-01 | 深圳迈瑞生物医疗电子股份有限公司 | Leukocyte classified counting reagent and leucocyte classification method |
| CN108414427A (en) * | 2017-02-10 | 2018-08-17 | 深圳市帝迈生物技术有限公司 | A kind of leucocyte classification reagent |
| CN110132908A (en) * | 2018-02-09 | 2019-08-16 | 深圳市帝迈生物技术有限公司 | A kind of cell detection kit and its application |
| CN110132908B (en) * | 2018-02-09 | 2023-09-15 | 深圳市帝迈生物技术有限公司 | Cell detection kit and application thereof |
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Application publication date: 20110216 |