CN101957347A - Method for detecting monosaccharide and oligosaccharide in edible mushroom - Google Patents
Method for detecting monosaccharide and oligosaccharide in edible mushroom Download PDFInfo
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- CN101957347A CN101957347A CN 201010268270 CN201010268270A CN101957347A CN 101957347 A CN101957347 A CN 101957347A CN 201010268270 CN201010268270 CN 201010268270 CN 201010268270 A CN201010268270 A CN 201010268270A CN 101957347 A CN101957347 A CN 101957347A
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Abstract
The invention provides a method for detecting the type and content of monosaccharide and oligosaccharide in edible mushroom, which is a method for measuring and analyzing by an ion chromatographic electrochemical detection means. The ion chromatogram obtained by the method can be used for determining the type and content of the monosaccharide in the edible mushroom according to peak migration time and peak area. The method realizes the accurate control over the monosaccharide and oligosaccharide components in edible mushroom.
Description
Affiliated technical field
The present invention relates to the separation detection technology of monose, oligosaccharides in a kind of edible fungi, be specifically related to the separation detection of chromatography of ions seven kinds of monose, oligosaccharides in the edible fungi.
Background technology
Along with the increase of people to the healthy food demand, edible fungi is subjected to people's attention gradually as the agricultural product that a class is rich in multiple nutritional components and unique flavor in recent years, and its output and consumption figure increase gradually.Sugar is as nutritional labeling important in the food, has heat supply, keeps that blood sugar is constant, the consumption that constitutes tissue, protecting liver and detoxication, saving body internal protein, keeps physiological function such as fat metabolism, and the content of sugar also indicates the product nutritive value; Sugar also is flavor substance important in the food simultaneously, the kind of natural small molecular sugar and content have directly determined organoleptic qualities such as the physicochemical property of numerous foods such as vegetables, fruit and color, therefore, the mensuration of monose, oligosaccharides is one of project main during edible fungi is analyzed.Traditional monose, the mensuration of oligosaccharide content adopt paper chromatography, silica G thin-layered chromatography, derivatization vapor-phase chromatography, gas chromatography-mass spectrography, direct differential refraction to detect more or high performance liquid chromatography, the capillary electrophoresis of the photometric detection of deriving carry out monose, oligosaccharides composition analysis.Yet all there is complicated operation in these methods, needs derivatization treatment, sensitivity is lower and shortcoming such as poor selectivity.Chromatography of ions-Electrochemical Detection is the instrument analytical method that development in recent years is got up, and directly compartment analysis is measured amino acid and saccharide compound, is applicable to the mensuration of monose, oligosaccharides, disaccharides, micromolecule oligosaccharides, and its advantage has sample pre-treatments simple; Need not to derive; Analysis time is short; Extra small sample feeding volume; Because post is imitated high, can realize that same separation condition is to multi-component analysis in the several samples.
Summary of the invention
The objective of the invention is to: the problem at contained monose, oligosaccharides kind and content detection in the present edible fungi exist provides a kind of monose, oligosaccharides method for quick.
For achieving the above object, the present invention adopts following technical scheme: a kind of fast detection method that detects monose in the edible fungi, oligosaccharides, the ICS-3000 type ion chromatograph that it is characterized in that instrument selection Dai An company, pillar is selected the MA1 post of Dai An company for use, moving phase is the sodium hydroxide solution of deionized water and 600mmol/L, both ratios are 20: 80, and flow velocity is 0.4mL/min.Monose in the edible fungi dry sample, oligosaccharides are soluble in the aqueous phase by 50 ℃ of ultrasonic Extraction, filter upward machine analysis after diluting suitable multiple then, transit time and the comparison of standard items collection of illustrative plates with the peak that obtains, promptly know the monose, the oligosaccharides kind that contain in the sample, the typical curve of bringing the peak area that obtains into each monose, oligosaccharides is promptly known the content of monose, oligosaccharides.
The present invention compares with other chromatography of ions detection modes can detect seven kinds of monose, oligosaccharides simultaneously, has enlarged detectable sample type greatly.The invention solves the difficult problem that trehalose peak and sweet mellow wine peak can not separate fully simultaneously in other ion chromatographic separation patterns.Carbohydrate ion chromatography method commonly used can not make trehalose separate fully with sweet mellow wine, and this can not be to the accurate quantitative problem of these the two kinds of monose in the identical product with regard to having caused.The foundation of the inventive method make the edible fungi sample particularly in the agaricus bisporus sample content of these two kinds of sugar be able to accurate mensuration, can provide accurate analytical method for the trophic analysis and the detection of sample, these special features of the present invention at home and abroad do not appear in the newspapers in the document.
The difference of the present invention and other chromatographies of ions sees Table 1:
The comparison of table 1 the present invention and other ion chromatographic method
| The present invention | Other chromatographies of ions | |
| Chromatographic column | MA1 | PA20 |
| Moving phase is used NaOH concentration | 600mM | 200mM |
| Deionized water and NaOH ratio in the moving phase | 20∶80 | 1∶99 |
| Trehalose is separated fully with sweet mellow wine | Energy | Not |
| Detect the monose kind | 7 kinds | 3～5 kinds |
| Range of application | Edible fungi, fruit, vegetables | Fruit, vegetables |
Detected object: monose, oligosaccharides that the present invention is primarily aimed in the edible fungi detect, and also can detect monose contained in the fruit and vegetable, oligosaccharides simultaneously.
Sensing range: the present invention is mainly used in edible fungis such as agaricus bisporus, pleurotus eryngii, Pleurotus nebrodensis, also can be applicable to some fruits and vegetables.
Description of drawings
Fig. 1 is the chromatography of ions spectrogram of seven kinds of monose, oligosaccharides 2.5 μ g/mL concentration sample introductions.
Embodiment
Get five parts of agaricus bisporus samples in Shandong, the place of production, Zhejiang, Wuhan, Shanghai and Fujian, carry out analyzing and testing by following operating process respectively.
70 ℃ of oven dry of sample are pulverized.Get the 0.1g dry sample, add the 10ml deionized water, 50 ℃ of following ultrasonic Extraction one hour, extract is carried out 1000 times of dilutions, dilution filters through filter, gets machine testing on the 1ml filtrate, and testing result sees Table 2.
Monose, oligosaccharides kind and content detection result in the agaricus bisporus dry sample of the different place of production of table 2
This method is good in 0.1 μ g/ml～20 μ g/ml scope internal linear, the R of seven kinds of Standard for Sugars curves
2Between 0.9975～0.9999, detect and be limited to 0.025 μ g/ml.
The description that protection scope of the present invention is not limited among the embodiment to be done, the modification that does not depart from the present invention program center all belongs to protection scope of the present invention.
Claims (5)
1. the detection method of monose, oligosaccharides in the edible fungi, it is characterized in that: to edible fungi sample oven dry abrasive dust, sample after the pulverizing is ultrasonic Extraction monose and oligosaccharides in 50 ℃ of water, the sugared extract of dilution behind the suitable multiple put into ion chromatograph analysis, judge monose, oligosaccharides kind and content in the different edible fungis according to the transit time at each peak and peak area.
2. the detection method of monose, oligosaccharides in the edible fungi according to claim 1, it is characterized in that: can detect seven kinds of monose, oligosaccharides simultaneously, be respectively glucose, trehalose, mannose, fucose, sweet mellow wine, fructose and ribose, with other ion chromatography methods mutually specific energy realize separating fully of trehalose and sweet mellow wine, can obtain the content of sugar in the peak area value substitution typical curve with the sugar that records.
3. the detection method of monose, oligosaccharides in the edible fungi according to claim 1 is characterized in that: described chromatography of ions is DIONEX ICS-3000, and pillar adopts the DIONEX CarboPac MA1 of company post, detects
System is an electrochemical detection system.
4. the detection method of monose, oligosaccharides in the edible fungi according to claim 1, it is characterized in that: the moving phase of using in the testing process is the sodium hydroxide solution of deionized water and 600mmol/L, and its ratio is a deionized water: sodium hydroxide solution=20: 80.
5. the detection method of monose, oligosaccharides in the edible fungi according to claim 1 is characterized in that: edible fungi sample powder to be detected only needs ultrasonic Extraction, dilution, filtration can go up machine testing, need not monose, oligosaccharides are carried out derivation process.
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| CN2010102682702A CN101957347B (en) | 2010-08-27 | 2010-08-27 | Method for detecting monosaccharide and oligosaccharide in edible mushroom |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104280472A (en) * | 2014-10-16 | 2015-01-14 | 江苏省农业科学院 | Method for detecting sugar components in fruits by liquid chromatography carried VWD (variable wavelength detector) |
| CN106596758A (en) * | 2016-12-04 | 2017-04-26 | 中国水稻研究所 | Method for simultaneously testing multiple free sugars in rice |
| CN106950311A (en) * | 2017-04-06 | 2017-07-14 | 北京中农弘科生物技术有限公司 | The assay method of mannan content in a kind of yeast culture |
| WO2018219258A1 (en) * | 2017-05-29 | 2018-12-06 | 青岛大学附属医院 | High performance liquid chromatography for free mannose and glucose in serum |
| CN109490429A (en) * | 2018-10-15 | 2019-03-19 | 江西诚志生物工程有限公司 | The detection method of glucose in a kind of d- ribose fermentation liquid |
| CN110514778A (en) * | 2019-09-25 | 2019-11-29 | 日照海关综合技术服务中心 | A kind of method that 22 kinds of sugar, sugar alcohol and alcohols detect simultaneously in fruit juice |
| CN110514777A (en) * | 2019-09-25 | 2019-11-29 | 日照海关综合技术服务中心 | A kind of method that a variety of sugar, sugar alcohols and alcohols quickly detect simultaneously in beer |
| CN111272895A (en) * | 2020-03-03 | 2020-06-12 | 中国水产科学研究院黄海水产研究所 | Method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed |
-
2010
- 2010-08-27 CN CN2010102682702A patent/CN101957347B/en not_active Expired - Fee Related
Non-Patent Citations (5)
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| 《Journal of Chromatography A》 20051231 C´edric Guignard，et al Analysis of carbohydrates in plants by high-performance anion-exchange chromatography coupled with electrospray mass spectrometry 第137－142页 1－5 第1085卷, * |
| 《Journal of Microbiological Methods》 19971231 John E. Hallsworth，et al A rapid HPLC protocol for detection of polyols and trehalose 第7－13页 1－5 第29卷, * |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104280472A (en) * | 2014-10-16 | 2015-01-14 | 江苏省农业科学院 | Method for detecting sugar components in fruits by liquid chromatography carried VWD (variable wavelength detector) |
| CN106596758A (en) * | 2016-12-04 | 2017-04-26 | 中国水稻研究所 | Method for simultaneously testing multiple free sugars in rice |
| CN106950311A (en) * | 2017-04-06 | 2017-07-14 | 北京中农弘科生物技术有限公司 | The assay method of mannan content in a kind of yeast culture |
| CN106950311B (en) * | 2017-04-06 | 2019-08-06 | 北京中农弘科生物技术有限公司 | The measuring method of mannan content in a kind of yeast culture |
| WO2018219258A1 (en) * | 2017-05-29 | 2018-12-06 | 青岛大学附属医院 | High performance liquid chromatography for free mannose and glucose in serum |
| US10871473B2 (en) | 2017-05-29 | 2020-12-22 | The Affiliated Hospital Of Qingdao University | Detection of free mannose and glucose in serum using high performance liquid chromatography |
| CN109490429A (en) * | 2018-10-15 | 2019-03-19 | 江西诚志生物工程有限公司 | The detection method of glucose in a kind of d- ribose fermentation liquid |
| CN110514778A (en) * | 2019-09-25 | 2019-11-29 | 日照海关综合技术服务中心 | A kind of method that 22 kinds of sugar, sugar alcohol and alcohols detect simultaneously in fruit juice |
| CN110514777A (en) * | 2019-09-25 | 2019-11-29 | 日照海关综合技术服务中心 | A kind of method that a variety of sugar, sugar alcohols and alcohols quickly detect simultaneously in beer |
| CN111272895A (en) * | 2020-03-03 | 2020-06-12 | 中国水产科学研究院黄海水产研究所 | Method for simultaneously and rapidly detecting amino sugar, neutral sugar and uronic acid in seaweed |
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| CN101957347B (en) | 2012-11-14 |
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