CN101936911B - Bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions - Google Patents
Bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions Download PDFInfo
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- CN101936911B CN101936911B CN2010102490191A CN201010249019A CN101936911B CN 101936911 B CN101936911 B CN 101936911B CN 2010102490191 A CN2010102490191 A CN 2010102490191A CN 201010249019 A CN201010249019 A CN 201010249019A CN 101936911 B CN101936911 B CN 101936911B
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Abstract
The invention discloses a bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions. The developing liquid of the method is prepared by mixing the water solutions of colourless fluorescein, luminol, hydrogen peroxide, sodium citrate and ethylene diamine tetraacetic acid. The visual sensitivity is enhanced and the visual luminescence time is prolonged by changing the chemiluminescence wavelength, meanwhile the stains with strong fluorescence are formed in the bloodstain area to develop the unsharp bloodstain shape. After the bloodstain search and the preliminary position are carried out by using the luminescence, the yellow-green fluorescence stain corresponding to the bloodstain shape can be observed on the bloodstain position detected in preliminary by the luminescence method under the natural light, the lamplight or the ultraviolet light, then the following accurate position of the bloodstain by using the fluorescence and the vision is realized to facilitate the recording of the bloodstain shape and the sampling. The method can be popularized and used in the criminal cases investigation.
Description
Technical field
The invention belongs to by means of the chemical property of material and test or the analysis of material technical field, be specifically related to the bloodstain detection method of a kind of long wavelength's of having chemiluminescence and fluorescence and coloring function.
Background technology
Bloodstain be vestige be again material evidence, the dual nature of bloodstain also fully reflects its critical role and effect on the scene of a crime.As vestige, it can fully reflect the event trace after residing state when the injured party is killed and injured later active procedure and criminal touch bloodstain.As one of most important forensic, utilize the biological characteristics of bloodstain, promptly parameters such as the kind of bloodstain, blood group, sex and DNA can realize the identification of bloodstain source.Though the existing more research in bloodstain search and detection and localization aspect at the scene, because the influence of factors such as technological means and on-the-spot complicacy, still there are many difficulties in actual on-the-spot bloodstain search, location, bloodstain form and bloodstain collection.
The ultimate principle of all chemiluminescence bloodstain detection methods of having reported is; Can catalyzing hydrogen peroxide oxidation luminol based on haemoglobin in the bloodstain or protoheme; Generate a large amount of excited state aminophthalic acids in the bloodstain zone; When it returns ground state, be the blue light of 425nm with wavelength, and the formation light-emitting zone consistent with bloodstain form.Naked eyes identification through to this blue-light-emitting can realize bloodstain search and Primary Location, observes and writes down the light-emitting zone shape and can obtain bloodstain form information.Because naked eyes are low to the visual sensitivity of short-wavelength light, therefore, existing on-the-spot luminol chemiluminescence bloodstain detection method exists the vision fluorescent lifetime short; Usually have only tens of seconds time, be prone to shortcoming (Lisa Dilbeck, the Use of Bluestar Forensic in Lieu of Luminol at Crime Scenes of bloodstain omission; J.Forensic Iden., 2006,56 (5); 706-720.Filippo Barnia, Simon W.Lewis, Andrea Bertia; Gordon M.Miskellyc and Giampietro Lago, Forensic application of the luminol reaction as a presumptive test for latent blood detection, Talanta; 2007,72 (3), 896-913.).In addition; Existing chemiluminescence bloodstain detection method; Than fluorescence and development process clear and definite advantage is being arranged aspect the on-the-spot bloodstain search of complicacy, but then not enough to some extent aspect accurate location of bloodstain and bloodstain form record, this mainly is because the luminol reaction product does not have the clear and definite fluorescence and the effect that develops the color.Therefore, existing chemiluminescence bloodstain detection method is accurately located at bloodstain, is had clear and definite limitation aspect bloodstain form record and the follow-up bloodstain sampling.
The luminescence imaging method that bloodstain form reappears on the fabric had once been invented by the seminar at inventor place; And by State Intellectual Property Office's Grant Patent Right for Invention, the patent No. is that ZL 200610041756.6, denomination of invention are " the luminescence imaging method that bloodstain form reappears on the fabric ".This patent is to the residual micro-occult blood mark in yarn fabric washing back; Adopt the luminol chemiluminescence method; With being integrated into picture equipment records Weak-luminescence signal; Realization reappears the form of the potential bloodstain of denier on the yarn fabric, for the scene rebuilds with court's trial the material evidence foundation is provided, not as bloodstain method for searching open-air or complicated scene.
" luminol occult blood is found at the scene in application and research " delivered by people such as Hou Changyong (2009.9 (in) legal system society) disclosing used colour developing liquid in is: luminol solution 0.1g, massfraction are 30% oxydol 6mL, natrium carbonicum calcinatum 5g, distilled water 100mL.Detection method adopts sprayer having the bloodstain zone to spray colour developing liquid equably, and short wavelength's blue light is sent in the bloodstain zone, with camera Taking Pictures recording bloodstain form.The shortcoming that this detection method exists above-mentioned chemoluminescence method bloodstain to detect equally.
Summary of the invention
Technical matters to be solved by this invention is to overcome that above-mentioned detection method visual sensitivity is low, the vision fluorescent lifetime is short and the defective of the accurate location difficulty of bloodstain, provides that a kind of emission wavelength is long, fluorescent lifetime is long, and has the bloodstain detection method of the fluorescence and the accurate positioning function that develops the color simultaneously.
Solving the problems of the technologies described above the technical scheme that is adopted is made up of following step:
1, preparation colorless fluorescent cellulose solution
Take by weighing luciferin and place beaker, add NaOH and distilled water, stir and make its dissolving; Add zinc powder again, the mass ratio of luciferin and NaOH, zinc powder, distilled water is 1: 21: 30: 60, and heated and stirred to green fluorescence takes off to the greatest extent in boiling water bath; In the dark be placed to the solution clarification, elimination zinc and zinc paste residue change in the volumetric flask; Adding distil water, being mixed with amount of substance concentration is 1.0 * 10
-2The colorless fluorescent cellulose solution of mol/L adds 1～3 zinc granule, places 4 ℃ of preservations in the refrigerator.
2, preparation luminol solution
Take by weighing luminol, using amount of substance concentration to be mixed with amount of substance concentration as the sodium hydrate aqueous solution of 1mol/L is 1.0 * 10
-2The luminol solution of mol/L is stored in the brown bottle, and the lucifuge place preserves.
3, preparation luminol-leucofluorescein colour developing liquid
With amount of substance concentration is 1.0 * 10
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, the redistilled water that amount of substance concentration is 2mol/L mix by following parts by volume proportioning:
1～4 part of colorless fluorescent cellulose solution
1～2 part of luminol solution
1～2 part of sodium citrate aqueous solution
1～2 part of disodium ethylene diamine tetra-acetic acid aqueous solution
1～10 part of aqueous hydrogen peroxide solution
Redistilled water adds to 20 parts
Use pH value to 11.00～13.00 of amount of substance concentration, be mixed with luminol-leucofluorescein colour developing liquid as the sodium hydrate aqueous solution adjusting mixed liquor of 1mol/L.
4, detect bloodstain
Under dark situation; Luminol-leucofluorescein colour developing the liquid of step 3 preparation is sprayed at the material surface that has potential bloodstain equably with sprayer; Visual observation yellow green light-emitting zone is also confirmed its position, with the camera Taking Pictures recording light-emitting zone consistent with bloodstain form; Under light, natural light or ultraviolet light, observe the yellow-green fluorescence spot of above-mentioned yellow green light-emitting zone subsequently, further accurately write down bloodstain form and particular location thereof or carry out the bloodstain sampling.
The present invention prepares in luminol-leucofluorescein colour developing liquid step 3, is 1.0 * 10 with amount of substance concentration
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, redistilled water the best that amount of substance concentration is 2mol/L mix by following parts by volume proportioning:
2 parts of colorless fluorescent cellulose solutions
2 parts of luminol solutions
2 parts of sodium citrate aqueous solutions
1 part of disodium ethylene diamine tetra-acetic acid aqueous solution
2.5 parts of aqueous hydrogen peroxide solutions
Redistilled water adds to 20 parts
The pH value to 11.65 of using amount of substance concentration to regulate mixed liquor as the sodium hydrate aqueous solution of 1mol/L is mixed with luminol-leucofluorescein colour developing liquid.
The present invention adopts luminol-leucofluorescein colour developing liquid to detect and the location bloodstain; The haemoglobin catalyzing hydrogen peroxide can simultaneously that bloodstain is regional luminol oxidation form the excited state aminophthalic acid; Leucofluorescein is oxidized to coloured luciferin, but not the bloodstain zone still is a leucofluorescein.Coloured luciferin is accepted to be stimulated behind the energy of excited state aminophthalic acid, and the form of energy with optical radiation discharged, and produces long wavelength's yellow-green light in the bloodstain zone.Improved visual sensitivity, prolonged the vision fluorescent lifetime through changing the chemiluminescence wavelength; Form hyperfluorescence property color spot in the bloodstain zone simultaneously, make bloodstain form be able to clear manifesting.Utilize luminous carry out bloodstain search and Primary Location after; Can be under natural light, light or ultraviolet light; In the bloodstain position that above luminescence method is tentatively confirmed, observe the yellow-green fluorescence spot consistent with bloodstain form, realize the particular location of follow-up fluorescence and visual accurate location bloodstain; Provide convenience for writing down bloodstain form and sampling, can in the detection of public security criminal case, promote the use of.
Description of drawings
Fig. 1 is the camera Taking Pictures recording figure on the printer paper.
Fig. 2 is the camera Taking Pictures recording figure on the kraft.
Fig. 3 is the camera Taking Pictures recording figure on the oil painting paper.
Fig. 4 is the camera Taking Pictures recording figure on the tendon cloth.
Embodiment
To further explain of the present invention, but the invention is not restricted to these embodiment below in conjunction with accompanying drawing and embodiment.
Embodiment 1
Adopt the present invention following to bloodstain form method for visualizing on the printer paper:
1, preparation colorless fluorescent cellulose solution
Take by weighing luciferin 0.3333g and place the 250mL beaker, add 7g NaOH and 20g distilled water, stir and make its dissolving; Add the 10g zinc powder again, the mass ratio of luciferin and NaOH, zinc powder, distilled water is 1: 21: 30: 60, place boiling water bath; Heated and stirred to green fluorescence takes off to the greatest extent, in the dark is placed to the solution clarification, elimination zinc and zinc paste residue; Change in the volumetric flask, adding distil water, being mixed with amount of substance concentration is 1.0 * 10
-2The colorless fluorescent cellulose solution of mol/L adds 1～3 zinc granule, places 4 ℃ of preservations in the refrigerator.
2, preparation luminol solution
Take by weighing luminol 0.4419g, using amount of substance concentration to be mixed with amount of substance concentration as the sodium hydroxide solution of 1mol/L is 1.0 * 10
-2The luminol solution 250mL of mol/L stores in the brown bottle, and the lucifuge place preserves.
3, preparation luminol-leucofluorescein colour developing liquid
With amount of substance concentration is 1.0 * 10
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, the redistilled water that amount of substance concentration is 2mol/L mix by following volume proportion:
Colorless fluorescent cellulose solution 2mL
Luminol solution 2mL
Sodium citrate aqueous solution 2mL
Disodium ethylene diamine tetra-acetic acid aqueous solution 1mL
Aqueous hydrogen peroxide solution 2.5mL
Redistilled water adds to 20mL
The pH value to 11.65 of using amount of substance concentration to regulate mixed liquor as the sodium hydrate aqueous solution of 1mol/L is mixed with luminol-leucofluorescein colour developing liquid.
4, detect bloodstain
Get a slice size and be 3cm * unsharp printer paper of 4cm bloodstain form; Shakeout placement on the table; The luminol of in the darkroom, step 3 being prepared-leucofluorescein colour developing liquid is sprayed on the printer paper of bloodstain uniformly with sprayer; Produce yellowish green light in the bloodstain zone, get off the bloodstain form Taking Pictures recording that shows with camera.Under light, natural light, uviol lamp, observe the yellow-green fluorescence spot of above-mentioned yellow green light-emitting zone subsequently, further accurately write down bloodstain form and particular location thereof.
Present embodiment manifests original bloodstain form image to the unsharp printer paper of bloodstain form and sees Fig. 1; Wherein Fig. 1 a is an aspect graph picture before bloodstain form manifests; Fig. 1 b manifests aspect graph picture under the dark situation of back, and Fig. 1 c manifests aspect graph picture under the light of back, and Fig. 1 d manifests aspect graph picture under the uviol lamp of back.Visible by Fig. 1; After manifesting liquid spraying with luminol-leucofluorescein, under dark situation, there is comparatively significantly yellow-green light at original bloodstain place on the printer paper; Above-mentioned yellow green light-emitting zone has the yellow-green fluorescence spot under light, natural light, uviol lamp, and bloodstain form is high-visible.
Embodiment 2
Adopt the present invention following to bloodstain form method for visualizing on the printer paper:
In the present embodiment, be 1.0 * 10 with amount of substance concentration
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, the redistilled water that amount of substance concentration is 2mol/L mix by following volume proportion:
Colorless fluorescent cellulose solution 1mL
Luminol solution 1mL
Sodium citrate aqueous solution 1mL
Disodium ethylene diamine tetra-acetic acid aqueous solution 1mL
Aqueous hydrogen peroxide solution 1mL
Redistilled water adds to 20mL
The pH value to 11.65 of using amount of substance concentration to regulate mixed liquor as the sodium hydroxide solution of 1mol/L is mixed with luminol-leucofluorescein colour developing liquid.
Other steps are identical with embodiment 1.
Embodiment 3
Adopt the present invention following to bloodstain form method for visualizing on the printer paper:
In the present embodiment, be 1.0 * 10 with amount of substance concentration
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, the redistilled water that amount of substance concentration is 2mol/L mix by following volume proportion:
Colorless fluorescent cellulose solution 4mL
Luminol solution 2mL
Sodium citrate aqueous solution 2mL
Disodium ethylene diamine tetra-acetic acid aqueous solution 2mL
Aqueous hydrogen peroxide solution 10mL
Redistilled water adds to 20mL
The pH value to 11.65 of using amount of substance concentration to regulate mixed liquor as the sodium hydrate aqueous solution of 1mol/L is mixed with luminol-leucofluorescein colour developing liquid.
Other steps are identical with embodiment 1.
Embodiment 4
Adopt the present invention following to bloodstain form method for visualizing on the printer paper:
In the present embodiment, be 1.0 * 10 with amount of substance concentration
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, the redistilled water that amount of substance concentration is 2mol/L mix by following volume proportion:
Colorless fluorescent cellulose solution 3mL
Luminol solution 1.5mL
Sodium citrate aqueous solution 1.5mL
Disodium ethylene diamine tetra-acetic acid aqueous solution 1.5mL
Aqueous hydrogen peroxide solution 6mL
Redistilled water adds to 20mL
The pH value to 11.65 of using amount of substance concentration to regulate mixed liquor as the sodium hydrate aqueous solution of 1mol/L is mixed with luminol-leucofluorescein colour developing liquid.
Other steps are identical with embodiment 1.
Embodiment 5
Detect in the bloodstain step 4 at present embodiment, get a slice size and be 3cm * unsharp kraft of 4cm bloodstain form, other steps of this step are identical with embodiment 1.Other steps are identical with embodiment 1.
Present embodiment manifests original bloodstain form image to the unsharp kraft of bloodstain form and sees Fig. 2; Wherein Fig. 2 a is an aspect graph picture before bloodstain form manifests; Fig. 2 b manifests aspect graph picture under the dark situation of back, and Fig. 2 c manifests aspect graph picture under the light of back, and Fig. 2 d manifests aspect graph picture under the uviol lamp of back.Visible by Fig. 2; After manifesting liquid spraying with luminol-leucofluorescein, under dark situation, there is comparatively significantly yellow-green light at original bloodstain place on the kraft; Above-mentioned yellow green light-emitting zone has the yellow-green fluorescence spot under light, natural light, uviol lamp, and bloodstain form is high-visible.
Embodiment 6
Detect in the bloodstain step 4 at present embodiment, get a slice size and be the unsharp oil painting paper of 3cm * 4cm bloodstain form, other steps of this step are identical with embodiment 1.Other steps are identical with embodiment 1.
Present embodiment manifests original bloodstain form image to the unsharp oil painting paper of bloodstain form and sees Fig. 3; Wherein Fig. 3 a is an aspect graph picture before bloodstain form manifests; Fig. 3 b manifests aspect graph picture under the dark situation of back, and Fig. 3 c manifests aspect graph picture under the light of back, and Fig. 3 d manifests aspect graph picture under the uviol lamp of back.Visible by Fig. 3; After manifesting liquid spraying with luminol-leucofluorescein, under dark situation, there is comparatively significantly yellow-green light at original bloodstain place on the oil painting paper; Above-mentioned yellow green light-emitting zone has the yellow-green fluorescence spot under light, natural light, uviol lamp, and bloodstain form is high-visible.
Embodiment 7
Detect in the bloodstain step 4 at present embodiment, get a slice size and be 3cm * unsharp tendon cloth of 4cm bloodstain form, other steps of this step are identical with embodiment 1.Other steps are identical with embodiment 1.
Present embodiment manifests original bloodstain form image to the unsharp tendon cloth of bloodstain form and sees Fig. 4; Wherein Fig. 4 a is an aspect graph picture before bloodstain form manifests; Fig. 4 b manifests aspect graph picture under the dark situation of back, and Fig. 4 c manifests aspect graph picture under the light of back, and Fig. 4 d manifests aspect graph picture under the uviol lamp of back.Visible by Fig. 4; After manifesting liquid spraying with luminol-leucofluorescein, under dark situation, there is comparatively significantly yellow-green light at original bloodstain place on the tendon cloth; Above-mentioned yellow green light-emitting zone has the yellow-green fluorescence spot under light, natural light, uviol lamp, and bloodstain form is high-visible.
Embodiment 8
Detect in the bloodstain step 4 at embodiment 1～4, get the unsharp wood of bloodstain form or glass or aluminium flake or ceramic tile, other steps of this step are identical with embodiment 1.Other steps are identical with corresponding embodiment.
Embodiment 9
Detect in the bloodstain step 4 at embodiment 2～4, get the unsharp kraft of a slice bloodstain form or oil painting paper or tendon cloth, other steps of this step are identical with corresponding embodiment.Other steps are identical with corresponding embodiment.
Embodiment 10
In preparation luminol-leucofluorescein colour developing liquid step 3 of embodiment 1～9; Use amount of substance concentration to regulate the pH value to 11.00 of mixed liquor as the sodium hydrate aqueous solution of 1mol/L; Be mixed with luminol-leucofluorescein colour developing liquid, other steps of this step are identical with corresponding embodiment.Other steps are identical with corresponding embodiment.
Embodiment 11
In preparation luminol-leucofluorescein colour developing liquid step 3 of embodiment 1～9; Use amount of substance concentration to regulate the pH value to 13.00 of mixed liquor as the sodium hydrate aqueous solution of 1mol/L; Be mixed with luminol-leucofluorescein colour developing liquid, other steps of this step are identical with corresponding embodiment.Other steps are identical with corresponding embodiment.
In order to prove beneficial effect of the present invention; The inventor adopts the detection method (being called for short detection method of the present invention) of bloodstain in the embodiment of the invention 1 and the method (Hou Changyong that existing luminol chemiluminescence detects bloodstain; Bang east; Li Xue etc. the application of luminol during occult blood is found at the scene and research .2009.9 (in) legal system society) (being called for short the comparison and detection method) have carried out the contrast experiment, and the experiment situation is following:
One, preparation colour developing liquid
1, luminol-leucofluorescein colour developing liquid: used raw material and proportioning thereof are identical with embodiment 1.
2, used raw material and the proportioning thereof of contrast test colour developing liquid is following:
Luminol solution 0.1g
Massfraction is 30% oxydol 6mL
Natrium carbonicum calcinatum 5g
Distilled water 100mL
Two, detect bloodstain
Get size and be respectively 2 of 3cm * unsharp printer paper of 4cm bloodstain form, kraft, oil painting paper, tendon cloths; Wherein 1 is having the bloodstain place to spray luminol-leucofluorescein colour developing liquid uniformly with sprayer; Another sheet is having the bloodstain place to uniformly spray contrast test colour developing liquid with sprayer; The record luminous duration of bloodstain, and observe the bloodstain glow color, the result sees table 1.
Table 1 the inventive method and existing method comparing result detection time
| Comparison and detection method fluorescent lifetime (second) | Glow color | Detection method fluorescent lifetime of the present invention (second) | Glow color | |
| Printer paper | 62 | Blue light | 185 | Yellow-green light |
| Kraft | 65 | Blue light | 177 | Yellow-green light |
| Oil painting paper | 59 | Blue light | 163 | Yellow-green light |
| Tendon cloth | 55 | Blue light | 159 | Yellow-green light |
Visible by table 1, the method for long wavelength's chemiluminescence detection bloodstain of the present invention is with respect to the method for traditional luminol chemiluminescence detection bloodstain, and fluorescent lifetime has prolonged 3 times; Bloodstain zone jaundice green light; When detecting with existing luminol chemiluminescence method, bloodstain zone blue light-emitting, the wavelength coverage of blue light is between 400～460nm; The wavelength coverage of yellow-green light is between 500～590nm; Yellow-green light is compared wavelength with blue light obviously elongated, in visible wavelength range, more helps visual inspection.
Adopt the present invention to detect bloodstain; Under dark situation, there is yellow-green light in the bloodstain zone, utilizes luminous bloodstain search and the Primary Location of carrying out; Subsequently can be under natural light, light or ultraviolet light; In the bloodstain position that above luminescence method is tentatively confirmed, the visible yellow-green fluorescence spot consistent with bloodstain form realized the particular location of follow-up fluorescence and visual accurate location bloodstain.Traditional luminol chemiluminescence method detects bloodstain, can only utilize chemiluminescence to carry out bloodstain search and Primary Location, and under natural light, light or ultraviolet light, the luminol reaction product does not have clear and definite fluorescence and colour developing effect.
Claims (2)
1. the bloodstain detection method that has long wavelength's chemiluminescence and fluorescence and coloring function is characterized in that it is made up of following step:
(1) preparation colorless fluorescent cellulose solution
Take by weighing luciferin and place beaker, add NaOH and distilled water, stir and make its dissolving; Add zinc powder again, the mass ratio of luciferin and NaOH, zinc powder, distilled water is 1: 21: 30: 60, and heated and stirred to green fluorescence takes off to the greatest extent in boiling water bath; In the dark be placed to the solution clarification, elimination zinc and zinc paste residue change in the volumetric flask; Adding distil water, being mixed with amount of substance concentration is 1.0 * 10
-2The colorless fluorescent cellulose solution of mol/L adds 1～3 zinc granule, places 4 ℃ of preservations in the refrigerator;
(2) preparation luminol solution
Take by weighing luminol, using amount of substance concentration to be mixed with amount of substance concentration as the sodium hydroxide solution of 1mol/L is 1.0 * 10
-2The luminol solution of mol/L is stored in the brown bottle, and the lucifuge place preserves;
(3) preparation luminol-leucofluorescein colour developing liquid
With amount of substance concentration is 1.0 * 10
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, the redistilled water that amount of substance concentration is 2mol/L mix by following parts by volume proportioning:
1～4 part of colorless fluorescent cellulose solution
1～2 part of luminol solution
1～2 part of sodium citrate aqueous solution
1～2 part of disodium ethylene diamine tetra-acetic acid aqueous solution
1～10 part of aqueous hydrogen peroxide solution
Redistilled water adds to 20 parts
Use pH value to 11.00～13.00 of amount of substance concentration, be mixed with luminol-leucofluorescein colour developing liquid as the sodium hydrate aqueous solution adjusting mixed liquor of 1mol/L;
(4) detect bloodstain
Under dark situation; Luminol-leucofluorescein colour developing the liquid of step (3) preparation is sprayed at the material surface that has potential bloodstain equably with sprayer; Visual observation yellow green light-emitting zone is also confirmed its position, the light-emitting zone that the camera Taking Pictures recording is consistent with bloodstain form; Under light, natural light or ultraviolet light, observe the yellow-green fluorescence spot of above-mentioned yellow green light-emitting zone subsequently, further accurately write down bloodstain form and particular location thereof or carry out the bloodstain sampling.
2. according to the described bloodstain detection method of claim 1, it is characterized in that: in preparation luminol-leucofluorescein colour developing liquid step (3), be 1.0 * 10 amount of substance concentration with long wavelength's chemiluminescence and fluorescence and coloring function
-2The colorless fluorescent cellulose solution of mol/L, amount of substance concentration are 1.0 * 10
-2The luminol solution of mol/L, amount of substance concentration are that the sodium citrate aqueous solution of 0.1mol/L, the disodium ethylene diamine tetra-acetic acid aqueous solution that amount of substance concentration is 0.01mol/L, aqueous hydrogen peroxide solution, the redistilled water that amount of substance concentration is 2mol/L mix by following parts by volume proportioning:
2 parts of colorless fluorescent cellulose solutions
2 parts of luminol solutions
2 parts of sodium citrate aqueous solutions
1 part of disodium ethylene diamine tetra-acetic acid aqueous solution
2.5 parts of aqueous hydrogen peroxide solutions
Redistilled water adds to 20 parts
The pH value to 11.65 of using amount of substance concentration to regulate mixed liquor as the sodium hydrate aqueous solution of 1mol/L is mixed with luminol-leucofluorescein colour developing liquid.
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| EP3415503A1 (en) * | 2017-06-16 | 2018-12-19 | Korean National Police Agency | Composition for detecting bloodstain |
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| RU2605462C2 (en) * | 2014-03-03 | 2016-12-20 | Федеральное государственное казенное учреждение "Войсковая часть 34435" | Method for detecting traces of biological origin |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1369703A (en) * | 2001-02-12 | 2002-09-18 | 罗瑞彪 | Oxidative staining and fluorescent techniqe for detecting latent blood fingerprint |
| WO2003091687A3 (en) * | 2002-04-25 | 2004-04-08 | Roc Imp | Method and compound for detecting traces of blood |
| CN1811390A (en) * | 2006-02-07 | 2006-08-02 | 陕西师范大学 | Bloodstain morphological reappearing illumination imaging method on textile fabrics |
| WO2010145696A1 (en) * | 2009-06-16 | 2010-12-23 | Martin Jan Peter Eversdijk | Methods for detecting the presence or absence of blood |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3545398A1 (en) * | 1985-12-20 | 1987-06-25 | Boehringer Mannheim Gmbh | METHOD FOR INCREASING THE QUANTUM YIELD IN THE OXIDATION OF LUMINOL BY PEROXIDES IN THE PRESENCE OF PEROXIDASE |
-
2010
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1369703A (en) * | 2001-02-12 | 2002-09-18 | 罗瑞彪 | Oxidative staining and fluorescent techniqe for detecting latent blood fingerprint |
| WO2003091687A3 (en) * | 2002-04-25 | 2004-04-08 | Roc Imp | Method and compound for detecting traces of blood |
| CN1811390A (en) * | 2006-02-07 | 2006-08-02 | 陕西师范大学 | Bloodstain morphological reappearing illumination imaging method on textile fabrics |
| WO2010145696A1 (en) * | 2009-06-16 | 2010-12-23 | Martin Jan Peter Eversdijk | Methods for detecting the presence or absence of blood |
Non-Patent Citations (4)
| Title |
|---|
| 孙野.命案现场中血迹的鲁米诺和二氢荧光素搜寻方法探析.《吉林公安高等专科学校学报》.2006,第21卷(第4期),51-54. * |
| 王跃等.无色荧光素血潜印痕显现技术.《广东公安科技》.2006,(第1期),9-11. * |
| 王跃等.血潜印痕无色荧光素显现技术研究.《中国人民公安大学学报(自然科学版)》.2006,(第2期),15-16. * |
| 聂迎春等.被洗织物上血迹形态的成像重现研究.《陕西师范大学学报( 自然科学版)》.2008,第36卷(第1期),58-60. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3415503A1 (en) * | 2017-06-16 | 2018-12-19 | Korean National Police Agency | Composition for detecting bloodstain |
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| CN101936911A (en) | 2011-01-05 |
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