CN101921706A - Magnetic cell separator - Google Patents
Magnetic cell separator Download PDFInfo
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- CN101921706A CN101921706A CN2009100530524A CN200910053052A CN101921706A CN 101921706 A CN101921706 A CN 101921706A CN 2009100530524 A CN2009100530524 A CN 2009100530524A CN 200910053052 A CN200910053052 A CN 200910053052A CN 101921706 A CN101921706 A CN 101921706A
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- magnetic
- cell
- cellular segregation
- cell separator
- particle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention relates to a magnetic cell separator comprising a body, wherein the body is provided with at least one support member and a magnetic configuration member, wherein the support member is configured to vertically support a cell separation column; and the magnetic configuration member is at least partially arranged around the support member, is provided with two opposite surfaces in the radial direction of the cell separation column and comprises a plurality of laminated magnets and laminated magnetic substances clamped by the laminated magnets. The magnetic configuration member has dissimilar magnetic poles on the opposite surfaces; and the orientations of the laminated magnets for forming the magnetic configuration member are generally orthogonal with the vertically-supported cell separation column. By using a local magnetic field generated by a magnet composite body on the outer side of the cell separation column, the required cells can be quickly and efficiently trapped from the mixed solution and can be efficiently separated in the non-magnetic environment, and the high-purity sorting of specific cells can be realized. The invention can be used for separation and function research on various immune cells and steam cells.
Description
Technical field
The present invention relates to the cellular segregation field, relate more specifically to a kind of magnetic cell separator and the application aspect isolated cell thereof.
Background technology
Body is by organ and forming, and cell then is the fundamental unit of histoorgan.The basis of biology and medical science and clinical study often relate to different types of cell, particularly in the immunology research process, because immunocyte is the fundamental unit that the function of immune system of body is carried out, therefore, study the body functions of immune system, often at first need to separate these immunocytes.
The complicated component of immunocyte, its medium size lymphocyte comprises T, bone-marrow-derived lymphocyte, and the T cell comprises CD4+T cell, CD8+T cell, the CD4+T cell can be divided into CD4 again
+CD25
+T cell, CD4
+CD25
-T cell etc., and antigen presenting cell comprises dendritic cell, scavenger cell etc., by separating the cell of different subgroups, studies its directly performance immunological effect, or play a role by producing antibody, complement, cytokine etc.
Clinical biological cell therapeutical is the important method of disease treatment, the various clinical biological cell therapeutical has been widely used in multiple treatment of diseases, for example, bone marrow transplantation, peripheral blood hematopoietic stem cells are transplanted the first-selected methods of treatment that has become various neoplastic hematologic disorders, the dendritic cell treatment also has been proved to be methods of treatment rising in the tumor biotherapy, and one of important step of these researchs is the separation of cell.
Magnetic cell separator is the device that the magnetic particle that is scattered in the solution is applied magnetic field and captures.As a kind of method biomedical, immunology research, come the method for isolated cell may further comprise the steps by magnetic force: in the sepn process of cell, antigenic substance in the antibody that carries magnetic particle and the solution on the cell combines antigen-antibody reaction takes place, and cell combines thereby make in the antibody that carries magnetic particle and the solution accordingly.Then, the magnetic compound member by magnetic cell separator applies magnetic field, in the trapping solution with magnetic particle bonded cell.A kind of common mode is to be adsorbed onto on the magnetic conduction particle in the cellular segregation post by the magnetic particle that carries cell that swims in the solvent in the cellular segregation post at the magnet complex body of cellular segregation post outside configuration, then by washing absorption guide to the solvent on the magnetic conduction particle, non magnetic particle, thereby the cellular segregation that nonmagnetic material cell and magnetic particle carry is opened.
More existing in the market magnetic cell separators, yet also there are some shortcomings in these magnetic separator, for example automated cell sorter complex structure and non-automatic sorter mostly are single hole, are unfavorable for that cell carries out the positive and negative the selection, and cost an arm and a leg.
In sum, this area presses for the magnetic separator that development structure is simple, with low cost, adapt to wide (for example can carry out two above sample separation simultaneously).
Summary of the invention
The purpose of this invention is to provide a kind of simple in structure, magneticstrength is suitable, can be with the method for magnetic cell separator and a kind of usefulness magnetic cell separator isolated cell provided by the invention of the cell rapid acquiring of carrying magnetic particle in the cellular segregation post.
In a first aspect of the present invention, a kind of magnetic cell separator is provided, it comprises body, described body has:
At least one is configured to the bracing member with the support cellular segregation post that is vertically oriented, and at least in part around described bracing member configuration, thereby has respect to one another magnetic arrangement member in the radial direction at described cellular segregation post, described magnetic arrangement member is constituted by a plurality of stratiform magnet with by the stratiform magnetic substance of layered magnet clamping, described magnetic arrangement member locates to have different each other magnetic pole at described respect to one another, and constitutes the orientation and the described cellular segregation post cardinal principle quadrature that is vertically supported of described a plurality of stratiform magnet of described magnetic arrangement member.
In another kind of preference, described bracing member forms the form of notch, is used for embedding from the place ahead of described body described cellular segregation post.
In another kind of preference, described bracing member forms the jack of sealing, is used for inserting from the top of described body described cellular segregation post.
In another kind of preference, the notch that described bracing member forms or the diameter of jack are the 5-30 millimeter.
In another kind of preference, described body also disposes the stationary installation that is used for fixing described magnetic cell separator at the rear of described bracing member.
In another kind of preference, described stationary installation is the magnetic substance of the rear configuration of described bracing member, is used for by magnetic force magnetic cell separator being fixed in iron plate.
In another kind of preference, the size of described notch or jack adjustable.
In another kind of preference, the material that constitutes described magnetic substance is a permanent-magnet materials.
In another kind of preference, the described a plurality of stratiform magnets that constitute described magnetic arrangement member are rubidium iron boron.
In another kind of preference, described body has 2,3,4 or 6 described bracing members.
In another kind of preference, described magnetic arrangement member is folded by 2-20 stratiform magnet with by the stratiform magnetic substance of layered magnet clamping to be constituted.
In another kind of preference, described magnetic cell separator also comprises the container that is used for collecting cell separator column flowing liquid.
In a second aspect of the present invention, a kind of device that is used for cellular segregation is provided, described device comprises:
(a) magnetic cell separator described in first aspect present invention; With
(b) cellular segregation post, described cellular segregation post is made up of the three part column-shaped barrels that up and down both ends open, internal diameter successively decrease from top to bottom successively, wherein be used to fill the magnetic conduction particle in the second section column-shaped barrel, and the lower end surrounds magnetic conduction particle sieve with stainless (steel) wire thereon.
In a third aspect of the present invention, provide a kind of and carried out the method for cellular segregation with the cell separation apparatus described in magnetic cell separator described in the first aspect present invention or the second aspect present invention, it is characterized in that, comprise step:
(a) solution that contains cell to be separated is splined on the cellular segregation post,
Wherein, in described solution, described cell to be separated in conjunction with or be fixed in magnetic particle;
And described cellular segregation post is made up of the three part column-shaped barrels that up and down both ends open, internal diameter successively decrease from top to bottom successively, wherein is filled with the magnetic conduction particle in the second section column-shaped barrel, and the lower end surrounds the magnetic conduction particle with stainless (steel) wire and sieves thereon;
Described cellular segregation post inserts or embeds in the bracing member of described magnetic cell separator, thereby makes the second section column-shaped barrel be in the formed magnetic field of magnetic arrangement member of described magnetic cell separator;
Thereby make the magnetic particle that carries cell to be separated be adsorbed in described magnetic conduction particle by magnetic force;
(b) wash described cellular segregation post with elutriant; With
(c) the cellular segregation post is moved apart the magnetic field that described bracing member forms, make described magnetic particle under action of gravity, break away from the magnetic conduction particle, thereby obtain to contain the solution of the magnetic particle that carries described cell to be separated.
In another kind of preference, described method also comprises step (d): the solution that contains the magnetic particle that carries described cell to be separated is concentrated by centrifugal.
In should be understood that within the scope of the present invention, above-mentioned each technical characterictic of the present invention and specifically described in below (eg embodiment) each technical characterictic can make up mutually, thereby constitute new or optimized technical scheme.For example, with regard to the upper limit of a certain (as 10-100) on a large scale can with another preferably more among a small circle the lower limit 20 of (as 20-80) make up, thereby constitute another scope (for example 20-100), vice versa.As space is limited, this tired no longer one by one stating.
Description of drawings
Fig. 1 is the basic comprising sectional view of expression magnetic cell separator of the present invention.
In the magnetic cell separator that Fig. 1 represents, comprise that is supported a bracing member 1 that holds the cellular segregation post, relative two sides configuration is made of the stratiform magnetic compound of a plurality of stratiform magnet clamping about in the bracing member, and the magnetic pole of the opposite face of this magnet is a heteropolar magnetic arrangement member 2, form notch 3 in the described bracing member 1, be used to embed described cellular segregation post.Also dispose the stationary installation 4 that is used for fixing described magnetic cell separator at the rear of described bracing member.
Fig. 2 a is the expression basic comprising sectional view that can separate the magnetic cell separator of two above samples simultaneously of the present invention.The implication of Reference numeral is identical with Fig. 1 in Fig. 2 a.
Fig. 2 b is the outside view that can separate the magnetic cell separator of two above samples simultaneously.
Fig. 3 is the basic comprising sectional view of cellular segregation post.
Plant the cellular segregation post that has shown the device that is used for cellular segregation at Fig. 3, described cellular segregation post is made up of the three part column-shaped barrels that up and down both ends open, internal diameter successively decrease from top to bottom successively, be first part's column-shaped barrel 5, second section column-shaped barrel 6 and third part column-shaped barrel 7.Wherein be used to fill the magnetic conduction particle in the second section column-shaped barrel 6, and the lower end surrounds magnetic conduction particle sieve with stainless (steel) wire thereon.
Fig. 4 is in example of the present invention, the outside drawing when magnetic cell separator and cellular segregation post use.
Fig. 5 is in example of the present invention, the synoptic diagram of magnetic cell separator and cellular segregation post.
Embodiment
The inventor provides a kind of magnetic separator simple in structure first through extensive and deep research.This magnetic separator have a body, this body comprises by at least one group and supports the bracing member that holds the cellular segregation post, the stratiform magnetic compound by the clamping of a plurality of stratiform magnet in each member constitutes.By the stratiform magnetic compound, the present invention can provide intensity more uniform magnetic field, thereby can isolate magnetic particle-cell complexes effectively.
As used herein, the magnet grains of biomaterial that term " magnetic particle " has referred to surface coverage, magnetic particle can prepare by the commercial sources purchase or by ordinary method.
As used herein, term " magnetic particle-cell complexes " or " mixture of magnetic particle-cell " are used interchangeably, finger combines formed " magnetic particle-antibody-antigen-cell " mixture by " antibody-antigen " between the antigen on the antibody on the magnetic particle and the cell to be separated.
As used herein, term " magnetic conduction particle " refers to be used to fill the irony particle of separator column, the surface coverage biomaterial, and its effect provides the area that " magnetic particle-cell complexes " is detained.The magnetic conduction particle can prepare by the commercial sources purchase or by ordinary method.Common magnetic conduction particle comprises the particle of irony, stainless steel.
Magnetic cell separator
The invention provides a kind of magnetic cell separator that can be used for capturing " magnetic particle-cell complexes " in cylindrical (being similar to the cylinder bodily form) cellular segregation post.Magnetic separator of the present invention is specially adapted to occasions such as testing laboratory.
In magnetic cell separator of the present invention, comprise that at least one group is supported the bracing member hold the cellular segregation post, in each bracing member about relative two sides configuration constitute by the stratiform magnetic compound of a plurality of stratiform magnet clamping.
In another kind of preference, relative two sides configuration is made of the stratiform magnetic compound of a plurality of stratiform magnet clamping about in each bracing member, and the magnetic pole of the opposite face of this magnet is a heteropolar magnet composite body member, and the direction that its magnet complex body is provided with and the cellular segregation post of insertion be quadrature substantially.
In the another kind of preference of the present invention, described magnetic compound is made up of the magnet that certain magnetic field intensity can be provided, and the magnetic field of efficient capture cell is provided.Usually, magnetic compound can provide 1000-10000 Gauss the magneticstrength of (preferably 3000-8000 Gauss) in notch.
In the present invention, dispose magnetic compound in the mode that embeds the cellular segregation post in the described magnetic arrangement member.The hole that can insert the cellular segregation post can be closed hole, and forms such as the groove that does not seal are to hold the cellular segregation post of cylindricality.
In another kind of preference of the present invention, the rear in the described bracing member also disposes magnetic substance, by the rear side magnetic substance whole magnetic cell separator is fixed on the vertical support irony side plate.
In the class preference of the present invention, described bracing member and polar material are all selected permanent-magnet materials for use, for example rubidium iron boron.
In the class preference of the present invention, described magnetic cell separator can carry out the separating experiment of a plurality of samples simultaneously.
In the class preference of the present invention, insertable cellular segregation post is that three column-shaped barrels that both ends open, internal diameter from top to bottom successively decrease successively about in the of are formed in the described bracing member, wherein fill the granuloplastic magnetic conduction particle sieve of magnetic conduction in the second section column-shaped barrel, when the cell of magnetic bead connection sieves by the magnetic conduction particle in the cellular segregation post in the bracing member groove, under the effect in magnetic field, be attracted to the magnetic conduction particle surface.
The cellular segregation post
The cellular segregation post is matching used with magnetic separator, be used to catch the parts by " antibody-antigen-reactive " formation " mixture of magnetic particle-cell ".
Can be used for cellular segregation post of the present invention is not particularly limited, it can be the cell separator of various routines, as long as this cell separator can hold magnetic conduction particle sieve, and can will be separated by magnetic conduction particle " magnetic particle-cell complexes " of adsorbing and the solution that contains non-required cell.
A kind of preferred cellular segregation post as shown in Figure 3.This cellular segregation post is made up of three column-shaped barrels that up and down both ends open, internal diameter successively decrease from top to bottom successively.
Wherein fill the magnetic conduction particle in the second section column-shaped barrel and form magnetic conduction particle sieve, when the cell of magnetic bead connection sieves by the magnetic conduction particle in the cellular segregation post in the bracing member groove, under the effect in magnetic field, be attracted to the magnetic conduction particle surface, realize the separation of marked by magnetic bead cell, can be used for the high efficiency separation of various kinds of cell simultaneously.
Use magnetic cell separator of the present invention, the magnetic particle bonded cell short period of time from mixing solutions in the cellular segregation post can be captured expeditiously; After the washing by washings (as RPMI1640, PBS solution), under non-magnetic environment, be able to high efficiency separation, realize high purity sorting specific cells.
Equipment for magnetic separation
The present invention also provides a kind of magnetic cell tripping device, and it comprises or is made up of the present invention above-mentioned magnetic cell separator and cellular segregation post.
The method of isolated cell
The present invention also provides the method for the described magnetic cell separator isolated cell of a kind of usefulness.Usually this method may further comprise the steps: the antibody that (1) will carry magnetic particle combines with corresponding cell in the solution; (2) will contain the cell of magnetic particle connection by the cellular segregation post in the described magnetic cell separator bracing member groove; (3) when the cellular segregation post leaves magnetic field, under action of gravity, break away from the magnetic conduction particle, separating magnetic particles connects cell and unlabelled cell.
Major advantage of the present invention comprises:
The first, magnetic separator provided by the invention can be adsorbed onto the cell high-efficient that carries magnetic particle in the solution in the support material of cellular segregation post at short notice;
The second, the present invention forms at least one group of magnetic support member that supports to hold the cellular segregation post together, rear in each member and about the lamellated magnetic compound that disposes by the clamping of a plurality of stratiform magnet of relative two sides constitute, and selected rubidium iron boron for use, therefore the present invention can provide the magnetic field of suitable intensity, and can be used for the magnetic particle diameter is that 50 nanometers are to micron-sized spherical particle;
Three, fill the magnetic conduction particle in the cellular segregation post in the second section column-shaped barrel, form magnetic conduction particle sieve, increased the surface-area of absorption magnetic bead, under external the action of a magnetic field, be easy to from solution, adsorb the magnetic conduction particle surface of cell in the cellular segregation post that carries magnetic particle nearby, avoid carrying the cell of magnetic particle rapidly along field direction kinetic mutual collision of long distance and cells injury; Use the separation efficiency of this cell magnetic separator can reach 94%, and can shorten disengaging time;
Four, the present invention can provide many groups to support the bracing member that holds the cellular segregation post simultaneously, can separate various kinds of cell simultaneously, to raise the efficiency, satisfies different needs.
Five, magnetic cell separator provided by the present invention is simple in structure, magneticstrength is suitable, can be with the cell rapid acquiring of carrying magnetic particle in the cellular segregation post.Existing in the market import automated cell sorter such as the autoMACS complex structure that costs an arm and a leg, and non-automatic sorter mostly is single hole such as MiniMACS, unit price is at 3500 or 8500 yuan, and the magnetic separator simple in structure, that can carry out two above sample separation simultaneously of the present invention's exploitation is with low cost, unit price below 1/3, adapts to wide at it.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, people such as Sambrook for example, molecular cloning: laboratory manual (NewYork:Cold Spring Harbor Laboratory Press, 1989) condition described in, or the condition of advising according to manufacturer.
Shown in Fig. 1, Fig. 2 a, Fig. 2 b, a kind of magnetic cell separator, it comprises at least one group of bracing member 1 that supports the cellular segregation post, relative two sides configuration is made of the stratiform magnetic compound and the cellular segregation post of a plurality of stratiform magnet (2-10 sheet) clamping about in described each member, and the magnetic pole of the opposite face of this magnet is the magnet configuration member 2 of heteropolar magnet complex body, and the direction that its magnet complex body is provided with and the cellular segregation post of insertion be quadrature substantially.Dispose magnetic compound in the mode that embeds the cellular segregation post in the described magnetic arrangement member, the hole that wherein can insert the cellular segregation post can be closed hole or the forms such as groove 3 of not sealing, to hold cylindrical container.The also configurable magnetic substance 4 in rear in the described bracing member is fixed in whole magnetic cell separator on the vertical rack side plate by the rear side magnetic substance; Bracing member and polar material are all selected permanent-magnet materials for use, for example rubidium iron boron.Magnetic cell separator can carry out the separating experiment of a plurality of samples simultaneously.
As shown in Figure 3, the cellular segregation post that is used for magnetic cell separator is that three column-shaped barrels 5,6,7 that both ends open, internal diameter from top to bottom successively decrease successively about in the of are formed, wherein fill the granuloplastic magnetic conduction particle sieve of magnetic conduction in the second section column-shaped barrel 6, when the cell of magnetic bead connection sieves by the magnetic conduction particle in the cellular segregation post in the bracing member groove, under the effect in magnetic field, be attracted to the magnetic conduction particle surface;
In the cell magnetic separator in the present embodiment, for the cell that will discrete group carries magnetic bead in the mixed solution in 1 captures the magnetic conduction particle surface of cellular segregation post effectively by magnetic force, to embed the mode distributed magnet complex body of cellular segregation post; Magnet complex body 1 and 2 is by a plurality of magnet 2a and magnetic substance 2b is alternately laminated forms, and the magnetic pole of the relative face of each magnet is made so-called heteropolar structure, and 2a and 2b are the magnet carrier of concave structure; The column-shaped barrel (Fig. 3) that cylindrical cellular segregation post is a both ends open about in the of, from top to bottom be divided into three parts (5,6,7) that internal diameter successively decreases successively, wherein fill the magnetic conduction particle in second section (6) column-shaped barrel, and surround magnetic conduction particle sieve by stainless (steel) wire at upper and lower ends.
Isolated cell
In the present embodiment, the magnetic separator in the employing embodiments of the invention 1 (Fig. 2 a) comes isolated cell, and concrete operations are as follows:
To separate mouse CD4+T cell is example, at first takes out mouse spleen and prepares cell, and behind the broken red corpuscle of Tris-NH4CL solution, 1000rpm * 10min is centrifugal, and red corpuscle is removed in the back, with PBS liquid washing 2 times.Obtain spleen lymphocyte, be made into 2 * 10
8Cell with the PBSE re-suspended cell of 200ul, adds the CD4 monoclonal antibody of the marked by magnetic bead of an amount of (50ul), and 4 ℃ of lucifuges were hatched 15 minutes.(as Fig. 4 or shown in Figure 5) on the vertical rack side plate is fixed in magnetic cell separator absorption.
According to the quantity and the purpose of required isolated cell, select a kind of of A type, Type B magnetic separator for use.Usually, cell quantity to be separated is many more, then should select the higher magnetic separator of magneticstrength for use, and for example, cell quantity is 2 * 10
8During cell, optional A type (configuration of two sides is made up of 4 stratiform flat thin magnets, and magneticstrength is 2500-4000 Gauss); Cell quantity is 2 * 10
9When cell is above, optional Type B (configuration of two sides is made up of 6 stratiform flat thin magnets, and magneticstrength is 3500-5000 Gauss).
Connect test tube under the cellular segregation post.After 4 ℃ of lucifuges are hatched 15 minutes, PBSE washing (1000rpm * 10min is centrifugal) is removed not and cell bonded magnetic bead, after cell is made into the 500ul system with PBSE, slowly add in the cellular segregation post of magnetic separator, when treating that liquid will flow to end, add 500ul PBSE again in separator column, after repeating totally three times, renew the spout that test tube is connected to the cellular segregation post, separator column is withdrawn the cell magnetic separator, with 1-3ml PBSE wash-out separator column, can get required CD4+T cell (positive back-and-forth method) after flowing liquid is centrifugal, the purity that FACS detects isolated cell is 94%.
Reference examples
To separate mouse CD4+T cell is example, at first takes out mouse spleen and prepares cell, Tris-NH
4Behind the broken red corpuscle of Cl solution, 1000rpm * 10min is centrifugal, and red corpuscle is removed in the back, with PBS liquid washing 2 times.Obtain spleen lymphocyte, be made into 2 * 10
8Cell with the PBSE re-suspended cell of 200ul, adds the CD4 monoclonal antibody of the marked by magnetic bead of an amount of (50ul), and 4 ℃ of lucifuges were hatched 15 minutes.(MiltenyiBiotec Inc) is fixed on the vertical rack side plate with the MiniMACS separator, connects test tube under the cellular segregation post.After 4 ℃ of lucifuges are hatched 15 minutes, PBSE washing (1000rpm * 10min is centrifugal) is removed not and cell bonded magnetic bead, after cell is made into the 500ul system with PBSE, slowly add in the cellular segregation post, when treating that liquid will flow to end, add 500ul PBSE again in separator column, after repeating totally three times, renew the spout that test tube is connected to the cellular segregation post, separator column is withdrawn MACS, with 1ml PBSE wash-out separator column, can get required CD4+T cell (positive back-and-forth method) after flowing liquid is centrifugal, the purity that FACS detects isolated cell is 88%.
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (10)
1. a magnetic cell separator is characterized in that it comprises body, and described body has:
At least one is configured to the bracing member with the support cellular segregation post that is vertically oriented, and at least in part around described bracing member configuration, thereby has respect to one another magnetic arrangement member in the radial direction at described cellular segregation post, described magnetic arrangement member is constituted by a plurality of stratiform magnet with by the stratiform magnetic substance of layered magnet clamping, described magnetic arrangement member locates to have different each other magnetic pole at described respect to one another, and constitutes the orientation and the described cellular segregation post cardinal principle quadrature that is vertically supported of described a plurality of stratiform magnet of described magnetic arrangement member.
2. magnetic cell separator as claimed in claim 1 is characterized in that, described bracing member forms the form of notch, is used for embedding from the place ahead of described body described cellular segregation post.
3. magnetic cell separator as claimed in claim 1 is characterized in that, described bracing member forms the jack of sealing, is used for inserting from the top of described body described cellular segregation post.
4. as claim 2 or 3 described magnetic cell separators, it is characterized in that the notch that described bracing member forms or the diameter of jack are the 5-30 millimeter.
5. magnetic cell separator as claimed in claim 1 is characterized in that, described body also disposes the stationary installation that is used for fixing described magnetic cell separator at the rear of described bracing member.
6. magnetic cell separator as claimed in claim 1 is characterized in that the material that constitutes described magnetic substance is a permanent-magnet materials.
7. magnetic cell separator as claimed in claim 1 is characterized in that, the described a plurality of stratiform magnets that constitute described magnetic arrangement member are rubidium iron boron.
8. magnetic cell separator as claimed in claim 1 is characterized in that, described body has 2,3,4 or 6 described bracing members.
9. a device that is used for cellular segregation is characterized in that, described device comprises:
(a) magnetic cell separator according to claim 1; With
(b) cellular segregation post, described cellular segregation post is made up of the three part column-shaped barrels that up and down both ends open, internal diameter successively decrease from top to bottom successively, wherein be used to fill the magnetic conduction particle in the second section column-shaped barrel, and the lower end surrounds magnetic conduction particle sieve with stainless (steel) wire thereon.
10. one kind is carried out the method for cellular segregation with described magnetic cell separator of claim 1 or the described cell separation apparatus of claim 9, it is characterized in that, comprises step:
(a) solution that contains cell to be separated is splined on the cellular segregation post,
Wherein, in described solution, described cell to be separated in conjunction with or be fixed in magnetic particle;
And described cellular segregation post is made up of the three part column-shaped barrels that up and down both ends open, internal diameter successively decrease from top to bottom successively, wherein is filled with the magnetic conduction particle in the second section column-shaped barrel, and the lower end surrounds the magnetic conduction particle with stainless (steel) wire and sieves thereon;
Described cellular segregation post inserts or embeds in the bracing member of described magnetic cell separator, thereby makes the second section column-shaped barrel be in the formed magnetic field of magnetic arrangement member of described magnetic cell separator;
Thereby make the magnetic particle that carries cell to be separated be adsorbed in described magnetic conduction particle by magnetic force;
(b) wash described cellular segregation post with elutriant; With
(c) the cellular segregation post is moved apart the magnetic field that described bracing member forms, make described magnetic particle under action of gravity, break away from the magnetic conduction particle, thereby obtain to contain the solution of the magnetic particle that carries described cell to be separated.
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| CN2009100530524A CN101921706A (en) | 2009-06-15 | 2009-06-15 | Magnetic cell separator |
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| CN2009100530524A CN101921706A (en) | 2009-06-15 | 2009-06-15 | Magnetic cell separator |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667049A (en) * | 2013-11-30 | 2014-03-26 | 成都欣华源科技有限责任公司 | Magnetic separation tool |
| CN108296013A (en) * | 2018-03-02 | 2018-07-20 | 浙江大学 | A kind of separator of superparamagnetic nanoparticle and its application |
| CN110106080A (en) * | 2019-06-11 | 2019-08-09 | 深圳海思安生物技术有限公司 | Cell separation apparatus |
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| CN2609931Y (en) * | 2003-04-05 | 2004-04-07 | 肖长锦 | Magnetic cell separation apparatus |
| CN101254482A (en) * | 2007-02-28 | 2008-09-03 | 株式会社日立高新技术 | Magnetic separator and analyzer using the same |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2609931Y (en) * | 2003-04-05 | 2004-04-07 | 肖长锦 | Magnetic cell separation apparatus |
| CN101254482A (en) * | 2007-02-28 | 2008-09-03 | 株式会社日立高新技术 | Magnetic separator and analyzer using the same |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667049A (en) * | 2013-11-30 | 2014-03-26 | 成都欣华源科技有限责任公司 | Magnetic separation tool |
| CN108296013A (en) * | 2018-03-02 | 2018-07-20 | 浙江大学 | A kind of separator of superparamagnetic nanoparticle and its application |
| CN108296013B (en) * | 2018-03-02 | 2023-11-14 | 浙江大学 | Separation device for superparamagnetic nanoparticles and application thereof |
| CN110106080A (en) * | 2019-06-11 | 2019-08-09 | 深圳海思安生物技术有限公司 | Cell separation apparatus |
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