CN101910164A - Diamido thiazole derivatives as protein kinase inhibitors - Google Patents

Diamido thiazole derivatives as protein kinase inhibitors Download PDF

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CN101910164A
CN101910164A CN2008801234697A CN200880123469A CN101910164A CN 101910164 A CN101910164 A CN 101910164A CN 2008801234697 A CN2008801234697 A CN 2008801234697A CN 200880123469 A CN200880123469 A CN 200880123469A CN 101910164 A CN101910164 A CN 101910164A
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M·A·拉伯里
C·S·波克
T·T·王
P·A·P·兰迪
T·J·古奇
M·A·西迪昆
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Abstract

The present invention relates to structure I new diamido thiazole derivatives, comprise the composition of described diamido thiazole derivatives and use described diamido thiazole derivatives to treat or prevent the method for proliferative disorders, anti-proliferative disorders, inflammation, sacroiliitis, central nervous system disorders, cardiovascular disorder, alopecia, neuronic disease, ischemic damage, virus infection, fungi infestation or the illness relevant with protein kinase activity.Formula (I), wherein M be-C (O)-,-C (S)-,-S (O)-,-S (O) 2-,-NHS (O) 2-,-OC (O)-or-NHC (O)-; Q is formula (II).

Description

Diamido thiazole derivatives as protein kinase inhibitors
Invention field
The present invention relates to new diamido thiazole derivatives, comprise the composition of described diamido thiazole derivatives and use described diamido thiazole derivatives to treat or prevent the method for proliferative disorders, anti-proliferative disorders, inflammation, sacroiliitis, central nervous system disorders, cardiovascular disorder, alopecia, neuronal disease, ischemia injury, virus infection, fungi infestation or the illness relevant with protein kinase activity.
Background of invention
Protein kinase is an enzyme family, the phosphorylation of the hydroxyl of specific tyrosine, Serine or threonine residues in the phosphorylated of its catalytic proteins, particularly catalytic proteins.Protein kinase is playing crucial effect aspect the adjusting of many kinds of cell processes, described cell processes comprises metabolism, cell proliferation, cytodifferentiation and cell survival.Uncontrolled propagation is the sign of cancer cells, and one of can following dual mode shows as the cell division cycle imbalance: make pungency gene hyperactivity hyperkinesia or make the Inhibit Genes inactivation.Protein kinase inhibitors, modulator or conditioning agent change kinase whose function, described kinases such as cell cycle protein dependent kinase (CDK), mitogen activated protein kinase (MAPK/ERK), Glycogen Synthase kinase 3 (GSK3 β), the outpost of the tax office (Chk) (for example, CHK-1, CHK-2 etc.) kinases, AKT kinases, JNK etc.The example of protein kinase inhibitors in WO 02/22610 A1, and by people such as Y.Mettey at J.Med.Chem., describe among the 46:222-236 (2003).
Cell cycle protein dependent kinase is a serine/threonine protein matter kinases, and it is cell cycle and cell proliferation motivating force behind.The mistake of CDK function is adjusted in many important noumenal tumours and takes place with high frequency.Indivedual CDK, for example CDK1, CDK2, CDK3, CDK4, CDK5, CDK6 and CDK7, CDK8 etc. have given play to unique effect, and can be classified as G1S phase enzyme or G2M phase enzyme in the cell cycle progress.CDK2 and CDK4 make us interested especially, because of its activity is regulated by wrong in many kinds of human cancers continually.The CDK2 activity is that progress is needed to the S phase by the G1 phase of cell cycle, and CDK2 is one of the crucial composition at the G1 outpost of the tax office.The outpost of the tax office is in order to the correct order that keeps cell cycle events and allow cell to respond to injury or to proliferation signal, and the forfeiture of correct pass card control helps tumour to take place in the cancer cells.The CDK2 approach takes place in the level affects tumour of tumor inhibitor function (for example, p52, RB and p27) and oncogene activation (cyclin E).Many reports prove that the coactivator of CDK2 (cyclin E) and inhibitor (p27) the two be respectively overexpression or cross low the expression in breast cancer, colorectal carcinoma, nonsmall-cell lung cancer, cancer of the stomach, prostate cancer, bladder cancer, non-Hodgkin lymphoma, ovarian cancer and other cancer.Verified, it is relevant with the overall survival difference with the rising of CDK2 activity level that their expression changes.These observationss make CDK2 and modularity approach thereof become the noticeable target of exploitation cancer therapy.
Reported in the literature that competitive organic molecule of many adenosines 5 '-triguaiacyl phosphate (ATP) and peptide are used for possible cancer therapy as the CDK inhibitor.United States Patent (USP) 6,413, the 23rd row-Di 15 hurdles the 10th, 974, the 1 hurdles row provide the abundant description about the mutual relationship of various CDK and they and various types of cancers.Flavones pyridine alcohol (Flavopiridol, as follows) is a kind of non-selective CDK inhibitor, and it is carrying out human clinical trial, people such as A.M.Sanderowicz, J.Clin.Oncol. at present 16: 2986-2999 (1998).
Figure BPA00001171830600021
Other known CDK inhibitor for example comprise olomoucine (people such as J.Vesely, Eur.J.Biochem., 224: 771-786 (1994)) but and Loews Wei Ting (roscovitine) (people such as l.Meijer, Eur.J.Biochem., 243: 527-536 (1997)).United States Patent (USP) 6,107,305 have described some pyrazolo [3, the 4-b] pyridine compounds as the CDK inhibitor.An illustrative of compounds that derives from ' 305 patents is:
Figure BPA00001171830600031
People such as K.S.Kim, J.Med.Chem. 45: 3905-3927 (2002) and WO 02/10162 disclose some aminothiazole compounds as the CDK inhibitor.
Another serial protein kinase is those that play an important role as the outpost of the tax office in the cell cycle progress.Cell cycle progress (such as in response to dna damage) takes place in the unsuitable time in outpost of the tax office prevention, and when cell is suppressed, keep the metabolic balance of cell, and in some cases can cell death inducing (apoptosis) when the demand to the outpost of the tax office is not met.Close card control and can occur in the G1 phase (before DNA is synthetic) and occur in the G2 phase, before entering mitotic division.
Have the outpost of the tax office of a series to monitor genomic integrity, and when the sensing dna damage, these " DNA damages the outpost of the tax office " block the cell cycle progress at G1 and G2 phase, and delay progress by the S phase.This behavior allows DNA repair process complete its task before the producer group is duplicated and separated this genetic material to new daughter cell subsequently.The inactivation that has been proved CHK1 can be transduceed from the signal of DNA-damage sensation mixture, to suppress the kinase whose activation of cell periodic protein B/Cdc2 (described activation meeting promoting mitosis enters), and eliminate the DNA damage or the endogenous dna that bring out because of carcinostatic agent and damage the inductive G2 of institute inhibition, and can cause and preferentially kill the damaged cell in the formed outpost of the tax office.Referring to, for example, people such as Peng, Science, 277: 1501-1505 (1997); People such as Sanchez, Science, 277: 1497-1501 (1997), Nurse, Cell, 91: 865-867 (1997); Weinert, Science, 277: 1450-1451 (1997); People such as Walworth, Nature, 363: 368-371 (1993); With people such as AI-Khodairy, Molec.Biol.Cell., 5: 147-160 (1994).
Can in cancer chemotherapy and radiotherapy scheme, provide application widely to the selectivity manipulation of closing card control in the cancer cells, and the conventional sign of the human cancer " genomic instability " that will be used as cancer cells destructive selectivity basis can be provided in addition.There are many factors to make CHK1 become DNA-and damage the crucial target that closes card control.For the illustrating of the inhibitor of CHK1 and function associated kinase such as CDS1/CHK2 (a kind of and CHK1 of recent findings cooperates and regulates the kinases that the S phase makes progress) (referring to people such as Zeng, Nature, 395:507-510 (1998); Matsuoka, Science, 282:1893-1897 (1998)) can be provided for the valuable new treatment entity of cancer therapy.
Another group kinases is a Tyrosylprotein kinase.Tyrosylprotein kinase can be (fully in the cell) of receptor type (have the extracellular, stride film and cell intracellular domain) or non-receptor type.Receptor type tyrosine kinase comprises having multiple bioactive many transmembrane receptors.In fact, about 20 different receptor type tyrosine kinase subfamilies have been identified.A Tyrosylprotein kinase subfamily that is named as the HER subfamily comprises EGFR (HER1), HER2, HER3 and HER4.Up to now, the part that obtains identifying of this acceptor subfamily comprises epidermal growth factor, TGF-α, amphiregulin, HB-EGF, β cytokine and heredity plain (heregulin).Another subfamily of these receptor type tyrosine kinases is the Regular Insulin subfamily, comprises INS-R, IGF-IR, IR, and IR-R.The PDGF subfamily comprises PDGF-α and beta receptor, CSFIR, c-kit and FLK-II.FLK family comprises kinases insert structure domain receptor (KDR), fetus liver kinases-1 (FLK-1), fetus liver kinases-4 (FLK-4) and fms sample Tyrosylprotein kinase-1 (flt-1).About going through of receptor type tyrosine kinase, referring to people such as Plowman, DN﹠amp; P 7 (6): 334-339,1994.
It is believed that at least a non-receptor protein tyrosine kinase, promptly LCK mediates from interactional signal the transduction in T-cell of cell surface protein (Cd4) with crosslinked resisting-Cd4 antibody.Be provided at Bolen about more going through of nonreceptor tyrosine kinase, Oncogene is among the 8:2025-2031 (1993).The non-receptor type Tyrosylprotein kinase also comprises many subfamilies, comprises Src, Frk, Btk, Csk, Abl, Zap70, Fes/Fps, Fak, Jak, Ack, and LIMK.In these subfamilies each all further is divided into multiple acceptor again.For example, the Src subfamily is a maximum subfamily, comprises Src, Yes, Fyn, Lyn, Lck, Blk, Hck, Fgr, and Yrk.The enzyme of Src subfamily generates implication with tumour.About more going through of the Tyrosylprotein kinase of non-acceptor type, referring to Bolen, Oncogene, 8: 2025-2031 (1993).
Except the effect aspect the cell cycle control, protein kinase also plays decisive role in vasculogenesis, and vasculogenesis is to rely on its mechanism by the existing new capillary vessel of vascularization.When needed, vascular system can generate new capillary bed so that keep the normal function of tissue and organ.Yet in the grownup, vasculogenesis is fully suppressed, and only takes place when wound healing and the endometrial neovascularization of intermenstrual period.On the other hand, undesirable vasculogenesis is the sign of some diseases, such as retinopathy, psoriatic, rheumatoid arthritis, relevant macular degeneration and cancer (solid tumor) of age.Three members that the protein kinase that relates to angiogenesis comprises growth factor receptor tyrosine kinase family have been proved to be; VEGF-R2 (vascular endothelial growth factor receptor 2 is also referred to as KDR (kinases insert structure domain receptor) and FLK 1); FGF-R (fibroblast growth factor acceptor); And TEK (being also referred to as Tie-2).
The VEGF-R2 that only expresses on endotheliocyte mediates follow-up signal transduction in conjunction with virtuous angiogenesis growth factor VEGF and the activation by kinase activity in its cell.Therefore, expection can cause the minimizing of vasculogenesis to the direct inhibition of the kinase activity of VEGF-R2, though in the presence of exogenous VEGF also be like this (referring to people such as Strawn, Cancer Res., 56: 3540-3545 (1996)), fail to mediate as shown in the signal transduction as using the VEGF-R2 mutant.People such as Millauer, Cancer Res., 56: 1615-1620 (1996).In addition, as if except mediation VEGF angiogenic activity, VEGF-R2 is inoperative in the grownup.Therefore, the selective depressant of expection VEGF-R2 kinase activity shows toxicity hardly.
Similarly, FGFR is in conjunction with angiogenesis growth factor aFGF and bFGF and mediate follow-up intracellular signal transduction.Propose recently, can in the solid tumor that reaches a certain size, play key effect aspect the induction of vascular generation such as the somatomedin of bFGF.People such as Yoshiji, Cancer Research, 57: 3924-3928 (1997).Yet different with VEGF-R2, FGF-R expresses in a plurality of different cellular types of whole body, and may play in adult other normal physiologic process or not play an important role.However, reported micromolecular inhibitor with the FGF-R kinase activity be administered systemically blocked in the mouse by bFGF inductive vasculogenesis, do not have tangible toxicity.People such as Mohammad, EMBO Journal, 17: 5996-5904 (1998).
TEK (being also referred to as Tie-2) is another kind of receptor tyrosine kinase of only expressing on endotheliocyte, has shown that it works in vasculogenesis.The combination of described factor angiogenin-1 causes the autophosphorylation of the kinase domain of TEK, and cause the signal transduction process, described signal transduction process seemingly mediates endotheliocyte and the endothelium interaction of sustenticular cell on every side, thereby promotes Neovascularized maturation.On the other hand, the effect of plain-1 couple of TEK of factor angiopoietin-2 antagonizing vessel generation seemingly and destruction vasculogenesis.People such as Maisonpierre, Science, 277: 55-60 (1997).
Kinases JNK belongs to mitogen-activated protein kinase (MAPK) superfamily.JNK plays decisive role in inflammatory reaction, stress reaction, cell proliferation, apoptosis and tumour generate.The JNK kinase activity can activate by multiple stimulator, and described stimulator comprises pro-inflammatory cytokine (TNF-α and interleukin 1), lymphocyte costimulatory receptor (CD28 and CD40), DNA-damageability chemical, radiation and Fas signal transduction altogether.Derive from the result that JNK shaves except that mouse and show, JNK relates to apoptosis-inducing and t helper cell differentiation.
Pim-1 is a kind of little serine/threonine kinase.The expression level that has detected Pim-1 in lymph and marrow malignant tumour raises, and Pim-1 is being confirmed as the prognostic markers thing of prostate cancer recently.K.Peltola, " Signaling in Cancer:Pim-1 Kinase and itsPartners ", Annales Universitatis Turkuensis, Sarja-Ser.D Osa-Tom.616, (on August 30th, 2005), Http:// kirjasto.utu.fi/julkaisupalvelut/annaalit/2004/D616.htmlPim-1 plays the effect of liability factor, and can stop the apoptosis of malignant cell.People such as K.Petersen Shay, Molecular Cancer Research 3: 170-181 (2005).
Aurora kinase (Aurora kinase) (aurora-A (Aurora-A), aurora-B (Aurora-B), aurora-C (Aurora-C)) is a serine/threonine protein matter kinases, and itself and human cancer be colorectal carcinoma, breast cancer and other solid tumor implication for example.Aurora-A (being also referred to as AIK sometimes) is considered to relevant with the protein phosphorylation incident of modulating the cell cycle.Particularly, aurora-A can work aspect the karyomit(e) clean cut separation in the control mitotic division process.The mistake of cell cycle is regulated and can be caused cell proliferation and other unusual.Find that in the human colon carcinoma tissue aurora-A, aurora-B, aurora-C are by overexpression.(referring to people such as Bischoff, EMBO J., 17: 3052-3065 (1998); People such as Schumacher, J.Cell Biol. 143: 1635-1646 (1998); People such as Kimura, J.Biol.Chem., 272: 13766-13771 (1997)).
C-Met is a kind of proto-oncogene, the tyrosine kinase receptor of their coding pHGF/dispersion factors (HGF/SF).C-Met albumen is mainly expressed in epithelial cell, and because its function, it also is called hepatocyte growth factor receptor or HGFR.When HGF/SF activation c-Met, c-Met can activate multiple kinase pathways again, comprises from Ras to Raf to Mek to the approach of mitogen-activated protein kinase ERK1 to transcription factor ETS1.The Met signal in the nosetiology of human cancer and malignant progression, involve to some extent (referring to people such as Birchmeier, Nature Reviews Molecular Cell Biology, 4:915-925 (2003); People such as Zhang, Journal of Cellular Biochemistry, 88:408-417 (2003); With people such as Paumelle, Oncogene, 21:2309-2319 (2002)).
The multiple p38 MAPK-dependent cell reaction of mitogen-activated protein kinase activated protein kinase 2 (MAPKAP K2 or MK2) mediation.MK2 is a kind of important cell internal modulation agent, (for example be modulated at many acute and chronic inflammation diseases, rheumatoid arthritis and inflammatory bowel) in the production of cytokines of implication, described cytokine such as tumor necrosis factor alpha (TNFa), interleukin 6 (IL-6) and interferon-gamma (IFNg).MK2 is present in the nucleus of unprovoked cell, and moves on to tenuigenin and make tuberin and HSP27 phosphorylation and activation when stimulating.MK2 also relate to heart failure, cerebral ischemic injury, stress endurance adjusting and the generation of TNF-α (referring to people such as Deak, EMBO. 17: 4426-4441 (1998); People such as Shi, Biol.Chem. 383: 1519-1536 (2002); Staklatvala., Curr.Opin.Pharmacol. 4: 372-377 (2004); With people such as Shiroto, J.Mol.Cell Cardiol. 38: 93-97 (2005)).
The effective protein kinase inhibitors of needs is treated or is prevented and unusual cell proliferation disease states associated.In addition, the expectation kinase inhibitor has high affinity to the target kinases, and has the highly selective with respect to other protein kinase.Can easily synthesize and be for example following those: the inhibitor of one or more protein kinases (such as CHK1, CHK2, VEGF (VEGF-R2), Pim-1, CDKs or CDK/ cyclin mixture), and the two inhibitor of receptor tyrosine kinase and nonreceptor tyrosine kinase as the micromolecular compound of the virtuous inhibitor of cell proliferation.
Summary of the invention
In one aspect, the invention provides the compound of formula (I):
Figure BPA00001171830600071
And pharmacy acceptable salt, solvate, ester, prodrug and steric isomer, wherein dotted line is represented that choose wantonly and other key, and wherein:
M is-C (O)-,-C (S)-,-S (O)-,-S (O) 2-,-NHS (O) 2-,-OC (O)-or-NHC (O)-;
Q is:
Figure BPA00001171830600072
R 1Be H, alkyl, thiazolinyl, alkynyl ,-(alkylidene group) m-aryl ,-(alkylidene group) m-cycloalkyl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl or-(alkylidene group) m-heterocycloalkenyl, wherein any aryl, cycloalkyl, heteroaryl, Heterocyclylalkyl or heterocycloalkenyl group can be randomly replaced by 3 substituting groups at the most on ring carbon atom or theheterocyclic nitrogen atom, described substituting group can be identical or different and be selected from halo, alkyl, thiazolinyl, alkynyl, haloalkyl, hydroxyalkyl ,-OR 6,-(alkylidene group) m-N (R 6) 2,-C (O) OR 6,-NHC (O) R 6,-C (O) N (R 6) 2,-S (O) 2R 7,-CN ,-OH-,-NO 2,-(alkylidene group) m-aryl ,-(alkylidene group) m-cycloalkyl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl and-(alkylidene group) m-heterocycloalkenyl; Wherein any aryl or heteroaryl substituting group group can be randomly replaced by maximum 5 substituting groups, and described substituting group can be identical or different and be selected from halo ,-OH, alkyl ,-C (O) OR 6,-N (R 6) 2,-NHC (O) R 6,-C (O) N (R 6) 2,-S (O) 2R 7,-CN ,-OH ,-NO 2And-the O-alkyl; Wherein any aryl, cycloalkyl, heteroaryl, Heterocyclylalkyl or heterocycloalkenyl group can randomly condense in aryl, cycloalkyl, heteroaryl, Heterocyclylalkyl or heterocycloalkenyl group;
R 2Each appearance be H independently, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O) R 6Or-(alkylidene group) m-N (R 6) 2, or R 2Form carbonyl group altogether with its ring carbon atom that is connected;
R 3Be H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2, or R 3And R 3aForm the cycloalkyl or the heterocycloalkyl of carbonyl group or volution altogether with the same carbon atom that it is connected separately;
R 3aBe H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2
R 5Each appearance be H independently, alkyl ,-(alkylidene group) m-aryl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl ,-(alkylidene group) m-N (R 6) 2,-(alkylidene group) m-OH ,-(alkylidene group) m-NHC (O) R 6, hydroxyalkyl, haloalkyl ,-C (O) R 6,-C (O) OR 6,-C (O)-(alkylidene group) m-N (R 6) 2,-(alkylidene group) m-NHC (O) R 6,-NHC (O) OR 6Or-NHS (O) 2R 7
R 6Each appearance be H independently, alkyl, haloalkyl, cycloalkyl, aryl, Heterocyclylalkyl or heteroaryl;
R 7Each appearance be H independently, alkyl, aryl, cycloalkyl or haloalkyl;
R 8Be H, alkyl ,-OH ,-O-alkyl or haloalkyl;
R 10Be H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O) R 6Or-(alkylidene group) m-N (R 6) 2, perhaps R 10And R 10aForm the cycloalkyl or the heterocycloalkyl of carbonyl group or volution altogether with the same carbon atom that it is connected separately;
R 10aBe H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2
R 11Each appearance be H independently, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2, or R 11Form carbonyl group altogether with its ring carbon atom that is connected;
R 12Each appearance be H independently, alkyl ,-(alkylidene group) m-aryl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl ,-S (O) 2R 7, haloalkyl, hydroxyalkyl ,-C (O) R 6Or-C (O) OR 6
Ar is arylidene or inferior heteroaryl, wherein said arylidene or inferior heteroaryl connect by any two its adjacent ring carbon atoms, and wherein said arylidene or inferior heteroaryl group can randomly be replaced by maximum 4 substituting groups, described substituting group can be identical or different and be independently selected from halo, alkyl, alkoxyl group, aryloxy ,-NH 2,-NH-alkyl ,-N (alkyl) 2,-SR 6,-S (O) R 7,-S (O) 2R 7,-C (O) R 6,-C (O) OR 6,-C (O) N (R 6) 2,-NHC (O) R 6, haloalkyl ,-CN and NO 2, make that working as Ar is the tetrahydrochysene naphthylidene, R 2And R 3Not hydrogen separately;
W is-N (R 12) 2-,-S-,-O-or-C (R 5) 2-, wherein be-C (R at W 5) 2-time, two R 5The same carbon atom that group is connected with them can form the cycloalkyl or the heterocycloalkyl of volution altogether, wherein this volution group can randomly be replaced by maximum 4 groups, described group can be identical or different and be selected from halo, alkyl, thiazolinyl, alkynyl, haloalkyl, hydroxyalkyl ,-OR 6,-(alkylidene group) m-N (R 6) 2,-C (O) OR 6,-NHC (O) R 6,-C (O) N (R 6) 2,-S (O) 2R 7,-CN ,-OH ,-NO 2,-(alkylidene group) m-aryl ,-(alkylidene group) m-cycloalkyl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl and-(alkylidene group) m-heterocycloalkenyl;
Y is H, halo, alkyl or-CN;
Described optional when not existing with other key, Z is-C (R 8)-or-N-; Described optional when existing with other key, Z is-C-;
Each appearance of m is 0 or 1 independently;
N is the integer of 0-2; With
P is 0 or 1.
In one aspect, the compound of described formula (I) (" diamido thiazole derivatives ") can be used as protein kinase inhibitors.
In one aspect of the method, described diamido thiazole derivatives can be used for treating or preventing proliferative disorders, antiproliferative illness, inflammation, sacroiliitis, central nervous system disorders, cardiovascular disorder, alopecia, neuronal disease, ischemia injury, virus infection, fungi infestation or the illness (respectively do for oneself " patient's condition ") relevant with protein kinase activity.
In one aspect of the method, the invention provides pharmaceutical composition, comprise at least a diamido thiazole derivatives and the pharmaceutically acceptable carrier of significant quantity.Described composition can be used for treating or preventing patient's the patient's condition.
In a further aspect, the invention provides the method for treatment or prevention patient's the patient's condition, described method comprises at least a diamido thiazole derivatives that the patient is given significant quantity.
In one aspect of the method, the invention provides treatment patient's method for cancer, described method comprises at least a diamido thiazole derivatives that the patient is given significant quantity.
In one aspect of the method, the invention provides treatment patient's method for cancer, described method comprises the other carcinostatic agent that the patient is given at least a diamido thiazole derivatives and at least a non-diamido thiazole derivatives, and dosage wherein is effective for the treatment cancer together the time.
Detailed Description Of The Invention
In one embodiment, the invention provides diamido thiazole derivatives and or its pharmacy acceptable salt, solvate, ester and the prodrug of formula (I).Described diamido thiazole derivatives can be used for treating or preventing patient's the patient's condition.
Definition and abbreviation
As above used and run through the disclosure, unless otherwise stated, following term should be interpreted with following implication:
" acyl group " be meant H-C (O)-, alkyl-C (O)-or cycloalkyl-C (O)-group, wherein each group as previously mentioned.The combination of parent fraction is undertaken by carbonyl.In one embodiment, acyl group comprises low alkyl group.The limiting examples of the carboxyl groups that is fit to comprises formyl radical, ethanoyl and propionyl.
" alkoxyl group " is meant alkyl-O-group, and alkyl group wherein as previously mentioned.The limiting examples of the alkoxy base that is fit to comprises methoxyl group, oxyethyl group, positive propoxy, isopropoxy and n-butoxy.The combination of parent fraction is undertaken by ether oxygen.
" alkoxy carbonyl " is meant alkyl-O-CO-group.The limiting examples of the alkoxycarbonyl groups that is fit to comprises methoxycarbonyl and ethoxy carbonyl.The combination of parent fraction is undertaken by carbonyl.
" alkyl " is meant aliphatic hydrocarbon groups, its can be straight chain or side chain, and in chain, comprise about 20 carbon atoms of about 1-.In one embodiment, alkyl group comprises about 12 carbon atoms of about 1-in chain.In another embodiment, alkyl group comprises about 6 carbon atoms of about 1-in chain.Side chain be meant one or more low-grade alkyl groups (such as, methyl, ethyl or propyl group) be connected in the alkyl chain of straight chain.Low alkyl group is meant the group that has about 6 carbon atoms of about 1-in chain, and it can be a straight or branched.Alkyl group can be unsubstituted or randomly be replaced by one or more substituting groups that described substituting group can be identical or different, and each substituting group is independently selected from halo, alkyl, aryl, cycloalkyl, cyano group, hydroxyl, alkoxyl group ,-S-alkyl, amino,-NH (alkyl) ,-NH (cycloalkyl) ,-N (alkyl) 2,-O-C (O)-alkyl ,-O-C (O)-aryl ,-O-C (O)-cycloalkyl, carboxyl and-C (O) O-alkyl.The limiting examples of the alkyl group that is fit to comprises methyl, ethyl, n-propyl, sec.-propyl, normal-butyl, sec-butyl, isobutyl-, the tertiary butyl, n-pentyl, isopentyl, neo-pentyl, n-hexyl, n-heptyl and n-octyl.In one embodiment, alkyl group is " C 1-C 6Alkyl group ", have 1-6 carbon atom.
" alkylaryl " is meant alkyl-arylidene-group, and alkyl wherein and arylidene are as previously mentioned.In one embodiment, alkylaryl comprises low-grade alkyl group.The limiting examples of the kiki fang alkyl group that is fit to is a tolyl.The combination of parent fraction is undertaken by arylene group.
" alkyl sulphonyl " is meant alkyl-S (O 2)-group.In one embodiment, the described moieties of alkyl sulphonyl group is low alkyl group (that is C, 1-C 6Alkyl).The combination of parent fraction is partly carried out by alkylsulfonyl.
" alkylthio " is meant alkyl-S-group, and alkyl group wherein as previously mentioned.The limiting examples of the alkylthio group that is fit to comprises methylthio group and ethylmercapto group.The alkylthio group is incorporated into parent fraction by its sulphur atom.
" thiazolinyl " is meant the aliphatic hydrocarbon groups that comprises at least one carbon-to-carbon double bond, its can be straight or branched and in chain, comprise about 15 carbon atoms of about 2-.In one embodiment, alkenyl group has about 12 carbon atoms of about 2-in chain; In another embodiment, alkenyl group has about 6 carbon atoms of about 2-in chain.Side chain be meant one or more low-grade alkyl groups (such as, methyl, ethyl or propyl group) be connected in the alkenylene chain of straight chain.Low-grade alkenyl is meant to have about 6 carbon atoms of about 2-in chain, can be straight or branched.Alkenyl group can be unsubstituted or randomly be replaced by one or more substituting groups that described substituting group can be identical or different, and each substituting group is independently selected from halo, alkyl, aryl, cycloalkyl, cyano group, alkoxyl group and-S (alkyl).The limiting examples of the alkenyl group that is fit to comprises vinyl, propenyl, n-butene base, 3-methyl but-2-ene base, positive pentenyl, octenyl and decene base.
" alkylidene group " is meant the alkyl group of above-mentioned definition, and wherein alkyl group hydrogen atom has replaced with key.The limiting examples of alkylidene group comprises-CH 2-,-CH 2CH 2-,-CH 2CH 2CH 2-,-CH 2CH 2CH 2CH 2-,-CH (CH 3) CH 2CH 2-,-CH (CH 3)-and-CH 2CH (CH 3) CH 2-.In one embodiment, alkylidene group has about 6 carbon atoms of 1-.In another embodiment, alkylidene group is a side chain.In another embodiment, alkylidene group is a straight chain.
" alkenylene " is meant by the alkenyl group from above-mentioned definition and removes a resulting double functional group of hydrogen.The limiting examples of alkenylene comprises-CH=CH-,-C (CH 3)=CH-and-CH=CHCH 2-.
" alkynyl " is meant the aliphatic hydrocarbon groups that comprises at least one carbon-to-carbon triple bond, its can be straight or branched and in chain, comprise about 15 carbon atoms of about 2-.In one embodiment, alkynyl group has about 12 carbon atoms of about 2-in chain; And in another embodiment, alkynyl group has about 4 carbon atoms of about 2-in chain.Side chain be meant one or more low-grade alkyl groups (such as, methyl, ethyl or propyl group) be connected in the alkynyl chain of straight chain.Low-grade alkynyl is meant to have about 6 carbon atoms of about 2-in chain, described chain can be straight chain or side chain.The limiting examples of the alkynyl group that is fit to comprises ethynyl, proyl, 2-butyne base and 3-methyl butynyl.Alkynyl group can be unsubstituted or randomly be replaced by one or more substituting groups that described substituting group can be identical or different, and each substituting group is independently selected from alkyl, aryl and cycloalkyl.
" alkynyl alkyl " is meant alkynyl-alkyl-group, and wherein said alkynyl and alkyl are as previously mentioned.In one embodiment, the alkynyl alkyl comprises low-grade alkynyl and low-grade alkyl group.The combination of parent fraction is undertaken by alkyl.The limiting examples of the alkynyl alkyl group that is fit to comprises the proyl methyl.
" aralkoxy " is meant aralkyl-O-group, and wherein said aromatic alkyl group as previously mentioned.The limiting examples of the aralkyl oxy group that is fit to comprises benzyl oxygen base and 1-or 2-naphthalene methoxyl group.Combination to parent fraction is undertaken by ether oxygen.
" aromatic alkoxy carbonyl " is meant aralkyl-O-C (O)-group.The limiting examples of the aromatic alkoxy carbonyl group that is fit to is a benzyl oxygen base carbonyl.The combination of parent fraction is undertaken by carbonyl.
" aralkyl " or " arylalkyl " is meant aryl-alkylidene group-group, and wherein said aryl and alkylidene group are as previously mentioned.In one embodiment, aralkyl comprises lower alkylene groups.The limiting examples of the aromatic alkyl group that is fit to comprises benzyl, 2-styroyl and naphthyl methyl.Combination to parent fraction is undertaken by alkylidene group.
" aromatic alkylthio " is meant aralkyl-S-group, and wherein said aromatic alkyl group as previously mentioned.The limiting examples of the aromatic alkylthio group that is fit to is a benzylthio-.Combination to parent fraction is undertaken by sulphur.
" aryl " be meant comprise about 14 carbon atoms of about 6-, preferably about 10 carbon atoms of about 6-the aromatic series monocycle or encircle ring system more.Described aromatic yl group can randomly be replaced by one or more " ring system substituting groups " and be as defined herein, and described " ring system substituting group " can be identical or different.The limiting examples of the aromatic yl group that is fit to comprises phenyl and naphthyl.
" arylidene " is meant aromatic yl group, and the hydrogen atom that wherein is connected in the ring carbon atom of an aromatic yl group is replaced by singly-bound.
" aryloxy " is meant aryl-O-group, and aromatic yl group wherein as previously mentioned.The limiting examples of the aryloxy group that is fit to comprises phenoxy group and naphthyloxy.Combination to parent fraction is undertaken by ether oxygen.
" aryloxy carbonyl " is meant aryl-O-C (O)-group.The limiting examples of the aryloxy carbonyl group that is fit to comprises phenyloxycarbonyl and naphthyloxy carbonyl.Combination to parent fraction is undertaken by carbonyl.
" aryl sulfonyl " is meant aryl-S (O 2)-group.Connection to parent fraction is undertaken by alkylsulfonyl.
" arylthio " is meant aryl-S-group, and aromatic yl group wherein as previously mentioned.The limiting examples of the arylthio group that is fit to comprises thiophenyl and naphthalene sulfenyl.Combination to parent fraction is undertaken by sulphur.
" benzo-fused cycloalkyl " is meant the cycloalkyl moiety that condenses in the above-mentioned definition of phenyl ring.The limiting examples of benzo-fused cycloalkyl is 2,3-indanyl and tetrahydrochysene naphthylidene.
" benzo-fused cycloalkenyl group " is meant the cycloalkenyl group part that condenses in the above-mentioned definition of phenyl ring.The limiting examples of benzo-fused cycloalkyl comprises indenyl.
" benzo-fused heterocyclic radical " is meant the heterocyclic radical part that condenses in the above-mentioned definition of phenyl ring.The limiting examples of benzo-fused heterocyclic radical comprises indolinyl and 2, the 3-Dihydrobenzofuranes.
" benzo-fused heteroaryl " is meant the heteroaryl moieties that condenses in the above-mentioned definition of phenyl ring.The limiting examples of benzo-fused heteroaryl is indyl, indazolyl, benzofuryl, quinolyl, isoquinolyl, benzothiazolyl, indyl, benzimidazolyl-and benzothienyl.
" composition " is meant the product of the appointment composition that comprises specified amount, and the spawn that directly or indirectly obtains from the combination of the appointment composition of specified amount.
" cycloalkyl " be meant comprise about 10 carbon atoms of about 3-, preferably about 10 carbon atoms of about 5-the non-aromatic monocycle or encircle ring system more.In one embodiment, cycloalkyl ring comprises about 7 annular atomses of about 5-.Group of naphthene base can randomly be replaced by " ring system substituting group " and be as defined above, and described " ring system substituting group " can be identical or different.Group of naphthene base can randomly condense in aryl, heteroaryl or heterocycloalkyl ring.The ring carbon atom of group of naphthene base can randomly close with two bonds with Sauerstoffatom, to form carbonyl group and to produce the cycloalkanes carboxyl groups.The limiting examples of the monocyclic cycloalkyl that is fit to comprises cyclopropyl, cyclopentyl, cyclohexyl, suberyl, ring pentanoyl, hexamethylene acyl group etc.The limiting examples of the polycyclic cycloalkyl that is fit to comprises naphthane base, norcamphyl, adamantyl etc.
" cycloalkylalkyl " is meant the cycloalkyl moiety that is connected in the above-mentioned definition of parent nucleus by moieties (as above definition).The limiting examples of the cycloalkylalkyl that is fit to comprises cyclohexyl methyl, adamantyl methyl etc.
" cycloalkenyl group " is meant the monocycle of non-aromatic or encircles ring system more, comprise about 10 carbon atoms of 3-and have carbon-to-carbon double bond at least one ring.In one embodiment, cycloalkenyl groups has about 10 ring carbon atoms of about 5-.In another embodiment, cycloalkenyl groups has about 7 ring carbon atoms of about 5-.Cycloalkenyl groups can randomly be replaced by one or more identical or different " ring system substituting group ", and as defined above.The limiting examples of the monocyclic cycloalkenyl group that is fit to comprises cyclopentenyl, cyclohexenyl, ring heptan-butadienyl etc.The limiting examples of the polycyclic cycloalkenyl group that is fit to is a norbornene.
" cycloalkenyl alkyl " is meant the cycloalkenyl group part that is connected in the above-mentioned definition of parent nucleus by moieties (as above definition).The limiting examples of the cycloalkenyl alkyl that is fit to comprises the cyclopentenyl methyl, cyclohexenyl methyl etc.
" significant quantity " or " treatment significant quantity " is meant in the diamido thiazole derivatives that effectively produces desired therapeutic, improvement, inhibition or preventive effect when suffering from patient's administration of the patient's condition and/or the other therapeutical agent or the amount of its composition.In combined therapy of the present invention, significant quantity can be meant the significant quantity of the reagent that each is independent or be meant described combination significant quantity as a whole, wherein the amount of all reagent of institute's administration is effectively together the time, but the component reagent in the wherein said combination may not be to exist with significant quantity respectively.
" halo " is meant-F ,-Cl ,-Br or-I.In one embodiment, halo be meant-Cl or-Br.In another embodiment, halo is meant-F.
" haloalkyl " is meant the alkyl group of above-mentioned definition, and wherein one or more hydrogen atoms of alkyl group have replaced with halogen.In one embodiment, halogenated alkyl group has 1-6 carbon atom.In another embodiment, halogenated alkyl group is replaced by 1-3 F atom.The limiting examples of halogenated alkyl group comprises-CH 2F ,-CHF 2,-CF 3,-CH 2Cl and-CCl 3
" heteroaryl " is meant aromatic monocycle or encircles ring system more, comprise about 14 annular atomses of about 5-, and wherein 1-4 annular atoms is O independently, N or S, and remaining annular atoms is a carbon atom.In one embodiment, heteroaryl groups has 5-10 annular atoms.In another embodiment, heteroaryl groups is monocyclic and has 5 or 6 annular atomses.Heteroaryl groups can randomly be replaced by one or more identical or different " ring system substituting group ", and as herein with undefined.Heteroaryl groups connects by ring carbon atom, and any nitrogen-atoms of heteroaryl can randomly be oxidized to corresponding N-oxide compound.Term " heteroaryl " also comprises the heteroaryl groups that condenses in the above-mentioned definition of phenyl ring.The limiting examples of heteroaryl comprises pyridyl, pyrazinyl, furyl, thienyl, pyrimidyl, pyridone (comprising the pyridone that N-replaces) isoxazolyl, isothiazolyl oxazolyl, thiazolyl, pyrazolyl, the furazan base, pyrryl, triazolyl, 1,2, the 4-thiadiazolyl group, pyrazinyl, pyridazinyl, quinoxalinyl, 2, the 3-phthalazinyl, the oxindole base, imidazo [1,2-a] pyridyl, imidazo [2,1-b] thiazolyl, benzo furazan base, indyl, azaindolyl, benzimidazolyl-, benzothienyl, quinolyl, imidazolyl, the Thienopyrimidine base, quinazolyl, the Thienopyrimidine base, pyrrolopyridinyl, imidazopyridyl, isoquinolyl, the benzo-aza indyl, 1,2, the 4-triazinyl, benzothiazolyl etc.Term " heteroaryl " also finger divides saturated heteroaryl moieties, such as for example, and tetrahydro isoquinolyl, tetrahydric quinoline group etc.In one embodiment, heteroaryl groups is unsubstituted.In another embodiment, heteroaryl groups is 5 yuan a heteroaryl.In another embodiment, heteroaryl groups is 6 yuan a heteroaryl.
As used in this article, term " inferior heteroaryl " is meant heteroaryl groups, and the hydrogen atom that wherein is connected in an annular atoms of heteroaryl groups is replaced by singly-bound.
" heteroarylalkyl " is meant the heteroaryl moieties that is connected in the above-mentioned definition of parent nucleus by moieties (as above definition).The limiting examples of the heteroaryl that is fit to comprises 2-pyridylmethyl, quinolyl methyl etc.
" heterocyclic radical " is meant the saturated monocycle of non-aromatic or encircles ring system more and comprise about 10 annular atomses of 3-, and the annular atoms of 1-4 wherein is O, S or N independently, and remaining annular atoms is a carbon atom.In one embodiment, the heterocyclic radical group has about 10 annular atomses of about 5-.In another embodiment, the heterocyclic radical group has 5 or 6 annular atomses.In ring system, adjacent oxygen and/or sulphur atom can not be arranged.Any-NH in the heterocyclic ring can exist with protected form, such as for example, as-N (BOC) ,-N (Cbz) ,-N (Tos) group etc.; This protected heterocyclic radical group also is considered to a part of the present invention.Term " heterocyclic radical " also comprises the heterocyclic radical group that condenses in the above-mentioned definition of aryl (for example, benzene) or heteroaryl ring.The heterocyclic radical group can randomly be replaced by one or more identical or different " ring system substituting group ", and as herein with undefined.The nitrogen of heterocyclic radical or sulphur atom can randomly be oxidized to corresponding N-oxide compound, S-oxide compound or S, S-dioxide.The limiting examples of monocyclic heterocyclic ring comprises piperidyl, pyrrolidyl, piperazinyl, morpholinyl, thio-morpholinyl, thiazolidyl, 1,4-dioxane base, tetrahydrofuran base, tetrahydro-thienyl, lactan, lactone etc.The ring carbon atom of heterocyclic radical group can functionalised and be carbonyl group.The illustrative example of this heterocyclic radical group is a pyrrolidone-base:
Figure BPA00001171830600161
In one embodiment, the heterocyclic radical group is unsubstituted.In another embodiment, the heterocyclic radical group is 5 yuan a heterocyclic radical.In another embodiment, the heterocyclic radical group is 6 yuan a heterocyclic radical.
" heterocyclic radical alkyl " is meant the heterocyclic radical part that is connected in the above-mentioned definition of parent nucleus by moieties (as above definition).The limiting examples of the heterocyclic radical alkyl that is fit to comprises piperidino methyl, piperazinyl methyl etc.
" heterocycloalkenyl " is meant the heterocyclic radical group of above-mentioned definition, and wherein the heterocyclic radical group comprises the two keys of 3-10 annular atoms and at least one intra-annular carbon-to-carbon double bond or carbon-nitrogen.In one embodiment, the heterocycloalkenyl group has 5-10 annular atoms.In another embodiment, heterocycloalkenyl group is monocyclic and has 5 or 6 annular atomses.The heterocycloalkenyl group can randomly be replaced by one or more ring system substituting groups, and wherein the ring system substituting group is as defined above.The nitrogen of heterocycloalkenyl or sulphur atom can randomly be oxidized to corresponding N-oxide compound, S-oxide compound or S, S-dioxide.The limiting examples of heterocycloalkenyl group comprises 1,2,3,4-tetrahydro pyridyl, 1,2-dihydropyridine base, 1,4-dihydropyridine base, 1,2,3,6-tetrahydro pyridyl, 1,4,5,6-tetrahydro-pyrimidine base, 2-pyrrolinyl, 3-pyrrolinyl, 2-imidazolinyl, 2-pyrazolinyl, glyoxalidine base, dihydro-oxazole base, Er Qing oxadiazole base, dihydro-thiazolyl, 3, the dihydrofuran base that 4-dihydro-2H-pyranyl, dihydrofuran base, fluorine replace, 7-oxabicyclo [2.2.1] heptenyl, dihydro-thiophene base, dihydro thiapyran base etc.The ring carbon atom of heterocycloalkenyl group can functionalised and be carbonyl group.The illustrative example of this heterocycloalkenyl group is:
Figure BPA00001171830600171
In one embodiment, the heterocycloalkenyl group is unsubstituted.In another embodiment, the heterocycloalkenyl group is 5 yuan a heterocycloalkenyl.
" heterocycloalkenyl alkyl " is meant the heterocycloalkenyl part that is connected in the above-mentioned definition of parent nucleus by moieties (as above definition).
It is pointed out that of the present invention to comprise in the heteroatomic ring system on the carbon atom adjacent, oh group can not be arranged, and on the carbon adjacent, N or S group can not be arranged with another heteroatoms with N, O or S.Therefore, for example in following ring:
Figure BPA00001171830600172
Can not have-OH is directly connected in and is labeled as 2 and 5 carbon.
It should be appreciated that, tautomeric form, such as for example, with the lower section:
Be considered to equal in certain embodiments of the invention.
" heteroaralkyl " is meant heteroaryl-alkyl-group, and wherein said heteroaryl and alkyl are as previously mentioned.In one embodiment, heteroaralkyl comprises low-grade alkyl group.The limiting examples of the aromatic alkyl group that is fit to comprises pyridylmethyl, and quinoline-3-ylmethyl.The combination of parent fraction is undertaken by alkyl.
" hydroxyalkyl " is meant the alkyl group of above-mentioned definition, and wherein one or more hydrogen atoms of alkyl group are replaced by-OH group.In one embodiment, the hydroxyalkyl group has 1-6 carbon atom.The limiting examples of hydroxyalkyl group comprises-CH 2OH ,-CH 2CH 2OH ,-CH 2CH 2CH 2OH and-CH 2CH (OH) CH 3
" patient " is people or inhuman Mammals.In one embodiment, the patient is the people.In another embodiment, the patient is the Mammals of non-human, includes but not limited to monkey, dog, baboon, rhesus monkey, mouse, rat, horse, cat or rabbit.In another embodiment, the patient is a companion animals, includes but not limited to dog, cat, rabbit, horse or ferret.In one embodiment, the patient is a dog.In another embodiment, the patient is a cat.
Term " purifying ", " purified form " or " through the form of separation and purifying " that is used for compound is meant that described compound is in the physical condition from building-up process (for example, separating from reaction mixture) or natural origin or its combination after separating.Therefore, being used for the term " purifying ", " purified form " of compound or " through separating and the form of purifying " is meant that described compound is from described or well known to a person skilled in the art that one or more purge processes (for example, chromatography, recrystallization etc.) are with can be by described or well known to a person skilled in the art that the sufficient purity that standard analytical techniques characterizes obtains physical condition afterwards herein herein.
" ring system substituting group " is meant the substituting group group that is connected in aromatic series or non-aromatic ring system, and described substituting group for example replaces an available hydrogen on the ring system.The ring system substituting group can be identical or different, is selected from alkyl independently of one another, thiazolinyl; alkynyl, aryl, heteroaryl;-alkyl-aryl ,-aryl-alkyl ,-alkylidene group-heteroaryl;-alkenylene-heteroaryl ,-alkynylene-heteroaryl, hydroxyl; hydroxyalkyl, haloalkyl ,-O-alkyl;-O-haloalkyl ,-alkylidene group-O-alkyl ,-O-aryl; aralkoxy, acyl group ,-C (O)-aryl; halo, nitro, cyano group; carboxyl ,-C (O) O-alkyl ,-C (O) O-aryl;-C (O) O-alkylidene group (alkelene)-aryl ,-S (O)-alkyl ,-S (O) 2-alkyl ,-S (O)-aryl ,-S (O) 2-aryl ,-S (O)-heteroaryl ,-S (O) 2-heteroaryl ,-S-alkyl ,-S-aryl ,-S-heteroaryl ,-S-alkylidene group-aryl ,-S-alkylidene group-heteroaryl, cycloalkyl, heterocyclic radical ,-O-C (O)-alkyl ,-O-C (O)-aryl ,-O-C (O)-cycloalkyl ,-C (=N-CN)-NH 2,-C (=NH)-NH 2,-C (=NH)-and NH (alkyl), Y 1Y 2N-, Y 1Y 2The N-alkyl-, Y 1Y 2NC (O)-and Y 1Y 2NSO 2-, Y wherein 1And Y 2Can be identical or different and be selected from hydrogen independently of one another, alkyl, aryl, cycloalkyl and-alkylidene group-aryl." ring system substituting group " can also be meant contemporary single part for two available hydrogen (H on each carbon) on two adjacent carbonss on the ring system.The example of this part is methylene-dioxy, ethylenedioxy ,-C (CH 3) 2-,-O-alkylidene group-O-etc., it forms such as for example following part:
Figure BPA00001171830600181
Term " substituted " is meant that the one or more hydrogen on the specified atom are replaced by the selection from designated groups, and condition is not surpass the normal valency of described specified atom under existing situation, and described replacement produces stable compound.The combination of substituting group and/or variable can be allowed to, as long as this combination obtains stable compound." stable compound " or " stable structure " are meant that compound is enough firm, can withstand from reaction mixture and be separated into useful purity, and be formulated as effective therapeutical agent.
Term " randomly substituted " is meant randomly and is replaced by described specified group, free radical or part.
It is pointed out that also valent any carbon atom or heteroatoms that having in text, scheme, embodiment and Biao is not met are assumed to be that the hydrogen atom with enough numbers is to satisfy described valency.
When the functional group in compound was described to " protected ", this was meant that this group is in adorned form, with the side reaction that prevents from not expect in the generation of protected position when compound reacts.The protecting group that is fit to is well known by persons skilled in the art, and the reference standard textbook is such as for example, people such as T.W.Greene, Protective Groups in Organic Synthesis (1991), Wiley, New York.
At any variable (for example, aryl, heterocycle, R 2Deng) when occur surpassing one time in any formation or any chemical structure described herein or chemical formula, its definition and its definition in all other situations when each situation has nothing to do.
The prodrug and the solvate of The compounds of this invention are also taken into account by this paper.Discussion about prodrug is provided at T.Higuchi and V.Stella, Pro-drugs as Novel DeliverySystems (1987) 14, the A.C.S.Symposium Series, and at BioreversibleCarriers in Drug Design, (1987) Edward B.Roche, the editor is among the AmericanPharmaceutical Association and Pergamon Press.Term " prodrug " is meant the compound (for example prodrug) that transforms in vivo with pharmacy acceptable salt, hydrate or the solvate that diamido thiazole derivatives or this compound are provided.(for example, by metabolism processing or chemical process) can take place by number of mechanisms in described conversion, such as for example, by hydrolysis in blood.About the discussion of the use of prodrug by T.Higuchi and W.Stella, " Pro-drugsas Novel Delivery Systems; " the A.C.S.Symposium Series, volume 14, and at Bioreversible Carriers in Drug Design, editor Edward B.Roche, AmericanPharmaceutical Association and Pergamon Press provides in 1987.
For example, if the pharmacy acceptable salt of diamido thiazole derivatives or this compound, hydrate or solvate comprise carboxylic acid functional, then prodrug can comprise that described group is such as for example (C by replace the formed ester of hydrogen atom of acidic group with following group 1-C 8) alkyl, (C 2-C 12) alkyloyl oxygen ylmethyl; 1-(alkyloyl oxygen base) ethyl with 4 to 9 carbon atoms; 1-methyl isophthalic acid-(alkyloyl oxygen base)-ethyl with 5 to 10 carbon atoms; alkoxy-carbonyl oxy methyl with 3 to 6 carbon atoms; 1-(alkoxy-carbonyl oxy) ethyl with 4 to 7 carbon atoms; 1-methyl isophthalic acid-(alkoxy-carbonyl oxy) ethyl with 5 to 8 carbon atoms; N-(alkoxy carbonyl) amino methyl with 3 to 9 carbon atoms; 1-(N-(alkoxy carbonyl) amino) ethyl with 4 to 10 carbon atoms; the 3-phthalidyl, 4-crotonolactone base, gamma-butyrolactone-4-base; two-N, N-(C 1-C 2) alkylamino (C 2-C 3) alkyl (such as β-dimethylaminoethyl), formamyl-(C 1-C 2) alkyl, N, N-two (C 1- 2) alkyl-carbamoyl-(C 1-C 2) alkyl and piperidino-(1-position only), pyrrolidyl or 4-morpholinyl (C 2-C 3) alkyl etc.
Similarly, if diamido thiazole derivatives comprises alcohol functional group, then can be by replacing the hydrogen atom of alcohol groups form prodrug with following group, described group is such as for example, (C 1-C 6) alkyloyl oxygen ylmethyl, 1-((C 1-C 6) alkyloyl oxygen base) ethyl, 1-methyl isophthalic acid-((C 1-C 6) alkyloyl oxygen base) ethyl, (C 1-C 6) the alkoxy-carbonyl oxy methyl, N-(C 1-C 6) the alkoxycarbonyl amino methyl, succinyl (succinoyl), (C 1-C 6) alkyloyl, alpha-amino group (C 1-C 4) alkyl, aryl-acyl and alpha-amino group acyl group, or-aminoacyl-alpha-amino group acyl group, wherein each alpha-amino group carboxyl groups is independently selected from naturally occurring L-amino acid, and P (O) is (OH) 2,-P (O) (O (C 1-C 6) alkyl) 2Or glycosyl (removing the free radical that an oh group obtains from the carbohydrate of hemiacetal formula) etc.
If diamido thiazole derivatives comprises amine functional group, then can form prodrug by the hydrogen of replacing amine groups with following group, described group is such as for example, the R-carbonyl, the RO-carbonyl, NRR '-carbonyl, wherein R and R ' they are (C independently of one another 1-C 10) alkyl, (C 3-C 7) cycloalkyl, benzyl, or the R-carbonyl is natural alpha-amino group acyl group or natural alpha-amino group acyl group ,-C (OH) C (O) OY 1(Y wherein 1Be H, (C 1-C 6) alkyl or benzyl) ,-C (OY 2) Y 3(Y wherein 2Be (C 1-C 4) alkyl and Y 3Be (C 1-C 6) alkyl, carboxyl (C 1-C 6) alkyl, amino (C 1-C 4) alkyl or list-N-or two-N, N-(C 1-C 6) the alkylamino alkyl) ,-C (Y 4) Y 5(Y wherein 4Be H or methyl and Y 5Be list-N-or two-N, N-(C 1-C 6) alkylamino morpholine subbase, piperidines-1-base or tetramethyleneimine-1-yl) etc.
One or more compounds of the present invention can be used as solvation not form and as and pharmaceutically acceptable solvent for example the solvation form of water, ethanol etc. exist, and be intended to solvation and not the form of solvation all be included in the present invention." solvate " is meant the physical bond of compound of the present invention and one or more solvent molecules.This physical bond relates to ionic linkage and covalent linkage in various degree, comprises hydrogen bond.In some cases, solvate can separate, for example in one or more solvent molecules are incorporated into situation in the lattice of crystalline solid." solvate " comprises solution phase solvent compound and separable solvate.The limiting examples of the solvate that is fit to comprises ethanol compound, methyl alcohol compound etc." hydrate " is that wherein solvent molecule is H 2The solvate of O.
One or more compounds of the present invention can randomly be converted into solvate.The preparation of solvate is normally known.Therefore, for example, people such as M.Caira, J.PharmaceuticalSci., 93 (3), 601-611 (2004) has described the solvate for preparing and prepare the antifungal drug fluconazole from water in ethyl acetate.The similar preparation method of solvate, half solvate, hydrate etc. is by people such as E.C.van Tonder, AAPS PharmSciTech., 5 (1), article 12 (2004); With people such as A.L.Bingham, Chem.Commun., 603-604 (2001) describes.Typical non-limiting method relates at solvent (organic solvent or water or its mixture) the dissolving The compounds of this invention of the temperature that is higher than envrionment temperature with the expectation of desired amount, and to be enough to form crystalline speed cooling solution, then by the standard method isolation of crystalline.Analytical technology, such as for example, I.R. spectroscopy shows the existence as solvent (or water) in the crystal of solvate (or hydrate).
Diamido thiazole derivatives can form salt, and described salt also within the scope of the present invention.Should be appreciated that, also comprise when describing diamido thiazole derivatives in this article and describe its salt, except as otherwise noted.As used in this article, the acid-salt that term " salt " expression and mineral acid and/or organic acid form, and the basic salt that forms with mineral alkali and/or organic bases.In addition, when diamido thiazole derivatives comprises basic moiety (such as, but not limited to pyridine or imidazoles) and acidic moiety (such as, but not limited to carboxylic acid) simultaneously, can form zwitter-ion (" inner salt "), it is also in the scope of term " salt " as used in this article.Preferably pharmaceutically acceptable (that is, nontoxic, physiology is acceptable) salt, but other salt also is useful.The salt of formula I compound can followingly form: for example make the acid of diamido thiazole derivatives and a certain amount of (such as monovalent) or alkali reacts in the sedimentary therein medium such as salt or water-bearing media in reaction and carry out lyophilize subsequently.
The exemplary acids additive salt comprises acetate, ascorbate salt, benzoate, benzene sulfonate, hydrosulfate, borate, butyrates, Citrate trianion, camphorate, camsilate, fumarate, hydrochloride, hydrobromate, hydriodate, lactic acid salt, maleate, mesylate, naphthalenesulfonate, nitrate, oxalate, phosphoric acid salt, propionic salt, salicylate, succinate, vitriol, tartrate, thiocyanate-, tosylate (being called tosylate again) etc.In addition, it has been generally acknowledged that the acid that is suitable for from the useful salt of basic medicinally compound formation pharmacy is by people such as for example P.Stahl, Camille G. (editor) Handbook of Pharmaceutical Salts.Properties, Selection and Use. (2002) Zurich:Wiley-VCH; People such as S.Berge, Journal ofPharmaceutical Sciences (1977) 66 (1)1-19; P.Gould, International J.ofPharmaceutics (1986) 33201-217; People such as Anderson, The Practice of MedicinalChemistry (1996), Academic Press, New York; And at The OrangeBook (Food ﹠amp; Drug Administration, Washington, D.C. is on its website) discuss.These openly are merged in this paper as a reference.
Exemplary alkaline salt comprises ammonium salt; An alkali metal salt is such as the salt of sodium, lithium and potassium; Alkaline earth salt is such as the salt of calcium and magnesium; With the salt of organic bases (for example, organic amine) formation, such as the salt of dicyclohexylamine, tert-butylamine; And with amino acids formed salt, such as the salt of arginine, Methionin etc.The group that comprises basic nitrogen such as elementary alkyl halide (for example can be used, the muriate of methyl, ethyl and butyl, bromide and iodide), dialkyl sulfate (for example, the vitriol of dimethyl, diethyl and dibutyl), long-chain halogenide (for example, the muriate of decyl, dodecyl and stearyl, bromide and iodide), aralkyl halide (for example, benzyl bromide and styroyl bromination thing) and other reagent carries out quaternized.
Being intended to all this acid-salts and basic salt all is the interior pharmacy acceptable salt of the scope of the invention, and for purpose of the present invention, all acid-salts and basic salt all are considered the free form that is equivalent to respective compound.
The pharmaceutically acceptable ester of The compounds of this invention comprises following group: the carboxylicesters that (1) esterification by oh group obtains; wherein the non-carbonyl moiety of the carboxylic moiety of ester group be selected from straight or branched alkyl (for example; ethanoyl, n-propyl, the tertiary butyl or normal-butyl), alkoxyalkyl (for example; methoxymethyl), aralkyl (for example; benzyl), aromatic yloxy yl alkyl (for example, phenoxymethyl), aryl is (for example; randomly by for example halo, C 1-4Alkyl, or C 1-4Alkoxyl group or the amino phenyl that replaces); (2) sulphonate is such as alkyl-or aralkyl alkylsulfonyl (for example, methane sulfonyl); (3) amino acid ester (for example, L-is valyl or the L-isoleucyl); (4) phosphonic acid ester and (5) single, two or triguaiacyl phosphate.Phosphoric acid ester can be further esterified, for example uses C 1-20Alcohol or its reactive derivatives, or with 2,3-two (C 6-24) acylglycerol.
Can there be (for example, as acid amides or imino-ether) with its tautomeric form in diamido thiazole derivatives and salt thereof, solvate, ester and prodrug.In this article, all this tautomeric forms all are considered a part of the present invention.
Diamido thiazole derivatives can comprise asymmetric center or chiral centre, and exists with different stereoisomeric forms in any ratio thus.Be intended to all stereoisomeric forms in any ratio of diamido thiazole derivatives and composition thereof, comprise racemic mixture, form a part of the present invention.In addition, the present invention comprises all geometrical isomers and positional isomers.For example, if diamido thiazole derivatives comprises two keys or condensed ring, then cis and trans forms and form of mixtures all are included in the scope of the invention.
Can by well known to a person skilled in the art method non-enantiomer mixture be separated into independent diastereomer based on its physical chemistry difference, such as for example passing through chromatography and/or fractional crystallization.Can following separation enantiomer: by with suitable optically active compound (for example, chiral auxiliary(reagent), alcohol or Mosher ' s acyl chlorides such as chirality) reaction enantiomeric mixture is converted into non-enantiomer mixture, separating diastereomer and independent diastereomer is transformed (for example, hydrolysis) is corresponding pure enantiomer.In addition, some diamido thiazole derivatives can be atropisomers (for example, substituted dibenzyl) and be considered to a part of the present invention.Enantiomer can also be separated by the HPLC post that uses chirality.
Also possible is that diamido thiazole derivatives can exist with different tautomeric forms, and all this forms all are included in the scope of the invention.In addition, all keto-enols and the imines-enamine form that comprise this compound in the present invention.
The compounds of this invention (the salt that comprises this compound, solvate, ester and prodrug, and the salt of prodrug, solvate and ester) all steric isomers (for example, geometrical isomer, optical isomer etc.), (comprise enantiomeric form (itself even can exist under the situation of asymmetric carbon not having) such as those that can exist owing to the asymmetric carbon on the different substituents, the rotational isomeric form, atropisomer, with the diastereomeric form formula), all be considered within the scope of the present invention, positional isomers also is the same (such as for example, 4-pyridyl and 3-pyridyl).(for example, if diamido thiazole derivatives comprises two keys or condensed ring, then cis and trans forms and form of mixtures all are included in the scope of the invention.In addition, for example, comprise all keto-enols and the imines-enamine form of this compound in the present invention.)
The independent steric isomer of The compounds of this invention can for example be substantially free of other isomer, perhaps can be blended, for example, mixes as racemoid or with all other steric isomers or other selected stereoisomers.Chiral centre of the present invention can have as defined S of IUPAC 1974Recommendations or R configuration.Be intended to similarly be applicable to salt, solvate, ester and the prodrug of enantiomer, steric isomer, rotational isomer, tautomer, positional isomers, racemoid or the prodrug of The compounds of this invention for the use of term " salt ", " solvate ", " ester ", " prodrug " etc.
The present invention also comprises isotope-labeled The compounds of this invention, it is identical with the compound of enumerating herein, and difference is that one or more atoms are had atomic mass or the total mass number atom different with atomic mass of usually finding at occurring in nature or total mass number and replace.Can be incorporated into the isotropic substance that isotopic example in the The compounds of this invention comprises hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine and chlorine, for example be respectively such as 2H, 3H, 13C, 14C, 15N, 18O, 17O, 31P, 32P, 35S, 18F and 36Cl.
Some isotope-labeled diamido thiazole derivatives (for example, is used 3H and 14Those of C mark) can be used for compound and/or substrate tissue distribution assays.Tritiate (that is, 3H) and carbon-14 (that is, 14C) isotropic substance is because easy preparation and detectability are particularly preferred.In addition, such as deuterium (that is, with higher isotope 2H) replacing some treatment interests (for example, transformation period or reduction dosage need in the extension body) that can realize by more greater metabolic stability brought therefore may be preferred in some cases also.Isotope-labeled diamido thiazole derivatives usually can by with following scheme and/or embodiment in the similar method of those disclosed prepare, replace nonisotopically labelled reagent with suitable isotope-labeled reagent.
The polymorphic form of the salt of described diamido thiazole derivatives and diamido thiazole derivatives, solvate, ester, prodrug and steric isomer is intended to be included in the scope of the invention.
The below following abbreviation of Shi Yonging, and have following implication:
Boc is a tert-butoxycarbonyl, and dba is two benzal benzylacetones, and DMF is N, dinethylformamide, DMSO are dimethyl sulfoxide (DMSO), and EtOAc is an ethyl acetate, LCMS is a liquid chromatography-mass spectrography, and MeOH is a methyl alcohol, and NMR is a nucleus magnetic resonance, PBS is a phosphate buffered saline (PBS), and SPA is a scintillation proximity assay, and Tf is fluoroform sulphonate/ester, TFA is a trifluoroacetic acid, with Xantphos be 9,9-dimethyl-4, two (diphenyl phosphine) xanthenes of 5-.
The diamido thiazole derivatives of formula (I)
The invention provides the diamido thiazole derivatives of formula (I):
Figure BPA00001171830600241
And pharmacy acceptable salt, solvate, ester, prodrug and steric isomer, wherein dotted line is represented that choose wantonly and other key, and R wherein 1, R 2, R 3, R 3a, R 10, R 10a, R 11, Ar, M, Q, W, Y, Z, n and p such as above-mentioned defined for formula (I).
In one embodiment, Q is:
Figure BPA00001171830600251
In another embodiment, Q is:
Figure BPA00001171830600252
In one embodiment, M be-C (O)-.
In another embodiment, M be-C (S)-.
In another embodiment, M be-OC (O)-.
In yet another embodiment, M be-S (O)-.
In another embodiment, M is-S (O) 2-.
In yet another embodiment, M is-NHS (O) 2-.
In further embodiment, M is-NHC (O)-.
In one embodiment, R 1Be-H.
In another embodiment, R 1Not H.
In another embodiment, R 1Be H, alkyl, heteroaryl, aryl ,-alkylidene group-aryl or Heterocyclylalkyl.
In yet another embodiment, R 1Be H, alkyl, heteroaryl, aryl ,-alkylidene group-aryl or Heterocyclylalkyl, wherein Heterocyclylalkyl can randomly be fused to phenyl ring, and wherein any aryl, heteroaryl or Heterocyclylalkyl can be randomly by three groups replacements at the most, and described group is selected from Heterocyclylalkyl independently of one another,-O-alkyl ,-O-haloalkyl and-OH.
In yet another embodiment, R 1Be phenyl, benzyl, 5 or 6 yuan of heteroaryls, 5 or 6 yuan of Heterocyclylalkyls, cycloalkyl, benzo-fused heteroaryl or benzo-fused Heterocyclylalkyl, they separately can be randomly by 3 groups replacements at the most, and described group is selected from halo independently of one another, alkyl ,-OH ,-NH 2, heteroaryl, or-the O-haloalkyl.
In another embodiment, R 1Be H, methyl, ethyl,
Figure BPA00001171830600261
In one embodiment, R 1It is alkyl.
In another embodiment, R 1It is thiazolinyl.
In another embodiment, R 1It is alkynyl.
In one embodiment, R 1It is aryl.
In another embodiment, R 1It is phenyl.
In another embodiment, R 1Be-alkylidene group-aryl.
In one embodiment, R 1It is heteroaryl.
In another embodiment, R 1Be-alkylidene group-heteroaryl.
In another embodiment, R 1It is pyrazinyl.
In another embodiment, R 1It is pyrazolyl.
In one embodiment, R 1Be:
Figure BPA00001171830600271
In one embodiment, R 1It is Heterocyclylalkyl.
In one embodiment, R 1It is heterocycloalkenyl.
In one embodiment, R 1Be-the alkylidenyl-heterocyclic alkyl.
In one embodiment, R 1Be-the alkylidenyl-heterocyclic thiazolinyl.
In one embodiment, R 1It is cycloalkyl.
In another embodiment, R 1Be-alkylidene group-cycloalkyl.
In one embodiment, R 1It is benzo-fused cycloalkyl.
In one embodiment, R 1It is benzo-fused heteroaryl.
In another embodiment, R 1It is benzo-fused Heterocyclylalkyl.
In further embodiment, R 1It is benzo-fused heterocycloalkenyl.
In one embodiment, M is-O-and R 1Be aryl ,-alkylidene group-aryl, cycloalkyl, heteroaryl or heterocycloalkenyl, they separately can be randomly by 3 groups replacements at the most, and described group is selected from halo independently of one another, alkyl ,-OH ,-NH 2, heteroaryl, or-the O-haloalkyl.
In another embodiment, M is-S-and R 1Be aryl ,-alkylidene group-aryl, cycloalkyl, heteroaryl or heterocycloalkenyl, they separately can be randomly by 3 groups replacements at the most, and described group is selected from halo independently of one another, alkyl ,-OH ,-NH 2, heteroaryl, or-the O-haloalkyl.
In one embodiment, M is-C (O)-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600272
In another embodiment, M is-NHC (O)-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600281
In one embodiment, R 2Be H.
In another embodiment, R 2Be alkyl.
In one embodiment, R 2For-CH 3
In another embodiment, R 2For-α-CH 3
In another embodiment, R 2For-β-CH 3
In further embodiment, R 2For-alkylidene group-NH 2
In one embodiment, R 2For-NH 2
In another embodiment, R 2For-α-NH 2
In another embodiment, R 2For-β-NH 2
In further embodiment, R 2For-alkylidene group-NH 2
In a further embodiment, R 2For-CH 2NH 2
In one embodiment, R 2Form carbonyl group with its carbon atom that is connected.
In one embodiment, R 3For-H.
In another embodiment, R 3aFor-H.
In another embodiment, R 3And R 3aAll be-H.
In another embodiment, R 3Be alkyl.
In another embodiment, R 3Be haloalkyl.
In a further embodiment, R 3Be hydroxyalkyl.
In one embodiment, R 3For-(alkylidene group) m-C (O) N (R 8) 2
In another embodiment, R 3For-(alkylidene group) m-NHC (O)-R 9
In another embodiment, R 3Be (alkylidene group) m-N (R 9) 2
In one embodiment, R 3For-CH 3
In another embodiment, R 3For-α-CH 3
In another embodiment, R 3For-β-CH 3
In one embodiment, R 3For-NH 2
In another embodiment, R 3For-α-NH 2
In another embodiment, R 3For-β-NH 2
In further embodiment, R 3For-alkylidene group-NH 2
In a further embodiment, R 3For-CH 2NH 2
In one embodiment, R 3And R 3aThe same carbon atom that is connected with them forms carbonyl group altogether.
In another embodiment, R 3And R 3aThe same carbon atom that is connected with them forms group of naphthene base altogether.
In another embodiment, R 3And R 3aThe same carbon atom that is connected with them forms heterocycloalkyl altogether.
In another embodiment, R 3And R 3aThe same carbon atom that is connected with them forms one of following group altogether:
Figure BPA00001171830600291
In one embodiment, R 2And R 2aAll be H.
In another embodiment, R 2Be alkyl and R 2aBe H.
In another embodiment, R 2Be H and R 2Be alkyl.
In one embodiment, R 10Be H.
In another embodiment, R 10aBe H.
In another embodiment, R 10And R 10aAll be H.
In another embodiment, R 10Be alkyl.
In another embodiment, R 10Be haloalkyl.
In a further embodiment, R 10Be hydroxyalkyl.
In one embodiment, R 10For-(alkylidene group) m-C (O) N (R 8) 2
In another embodiment, R 10For-(alkylidene group) m-NHC (O)-R 9
In another embodiment, R 10For-(alkylidene group) m-N (R 9) 2
In one embodiment, R 10For-CH 3
In another embodiment, R 10For-α-CH 3
In another embodiment, R 10For-β-CH 3
In one embodiment, R 10For-NH 2
In another embodiment, R 10For-α-NH 2
In another embodiment, R 10For-β-NH 2
In further embodiment, R 10For-alkylidene group-NH 2
In a further embodiment, R 10For-CH 2NH 2
In one embodiment, R 10And R 10aThe same carbon atom that is connected with them forms carbonyl group altogether.
In another embodiment, R 10And R 10aThe same carbon atom that is connected with them forms group of naphthene base altogether.
In another embodiment, R 10And R 10aThe same carbon atom that is connected with them forms heterocycloalkyl altogether.
In one embodiment, R 11Be H.
In another embodiment, R 11Be alkyl.
In one embodiment, R 11For-CH 3
In another embodiment, R 11For-α-CH 3
In another embodiment, R 11For-β-CH 3
In further embodiment, R 11For-alkylidene group-NH 2
In one embodiment, R 11For-NH 2
In another embodiment, R 11For-α-NH 2
In another embodiment, R 11For-β-NH 2
In further embodiment, R 11For-alkylidene group-NH 2
In a further embodiment, R 11For-CH 2NH 2
In another embodiment, R 11Form carbonyl group with its carbon atom that is connected.
In one embodiment, R 2, R 3, R 3a, R 10, R 10aAnd R 11All be H.
In another embodiment, R 2, R 3, R 10, R 10aAnd R 11All be H.
In another embodiment, R 2, R 10, R 10aAnd R 11All be H.
In another embodiment, R 3, R 3a, R 10, R 10aAnd R 11All be H.
In one embodiment, Ar be-arylidene-.
In another embodiment, Ar be-inferior heteroaryl-.
In another embodiment, Ar is:
Figure BPA00001171830600311
In a further embodiment, Ar is:
Figure BPA00001171830600312
In one embodiment, Ar is:
Figure BPA00001171830600313
In another embodiment, Ar is:
Figure BPA00001171830600314
In another embodiment, Ar is:
Figure BPA00001171830600321
In yet another embodiment, Ar is:
Figure BPA00001171830600322
In one embodiment, Ar is:
Figure BPA00001171830600323
Z is-N-and W be-N (R 12).
In yet another embodiment, Ar is:
Figure BPA00001171830600324
Z is-N-and W be-C (R 12).
In one embodiment, W is-C (NH 2) (C (O) NH 2)-.
In another embodiment, W is-C (NH 2) (alkyl)-.
In another embodiment, W is-C (NH 2) (CH 3)-.
In another embodiment, W is-C (NH 2) (C (O) NHOH)-.
In one embodiment, W is-CH (NC (O) CF 3)-.
In another embodiment, W is-CH (NS (O) 2Alkyl)-.
In another embodiment, W is-C (NH 2) (C (O) NHOH)-.
In one embodiment, W is-CH (CH 2NH 2)-.
In another embodiment, W is-C (C (O) NH 2) (NH alkyl)-.
In another embodiment, W is-CH (C (O) NH 2)-.
In another embodiment, W is-CH 2-.
In a further embodiment, W is-NH-.
In a further embodiment, W is-C (R 5) 2-.
In another embodiment, W is-CH (OH)-.
In another embodiment, W is-CH (NH 2)-.
In one embodiment, W is-CH (CH 3)-.
In another embodiment, W is-CH (C (O) CH 3)-.
In another embodiment, W be-C (OH) (alkyl)-.
In another embodiment, W be-C (OH) (alkylidene group-OH)-.
In another embodiment, W is-N (R 12)-.
In another embodiment, W is-O-.
In another embodiment, W is-S-.
In one embodiment, W is-C (R 5) 2-and two R 5The same carbon atom that group is connected with them forms group of naphthene base altogether.
In another embodiment, W is-C (R 5) 2-and two R 5The same carbon atom that group is connected with them forms heterocycloalkyl altogether.
In another embodiment, W is-C (R 5) 2-and two R 5The same carbon atom that group is connected with them forms the group of following formula altogether:
Figure BPA00001171830600331
In one embodiment, W is-C (R 5) 2-and each R 5Group is independently selected from H ,-(alkylidene group) m-NH 2,-NH-alkyl ,-N (alkyl) 2,-C (O) NH 2,-OH ,-C (O) O-alkyl, 5 or 6 yuan of heteroaryls or hydroxyalkyl.
In another embodiment, W is-C (R 5) 2-and each R 5Group is independently selected from H ,-(alkylidene group) m-NH 2,-NH-alkyl ,-N (alkyl) 2Or-C (O) NH 2
In one embodiment, Y is-H.
In another embodiment, Y is-halo ,-alkyl or-CN.
In another embodiment, Y is a methyl.
In one embodiment, Z is-CR 8-.
In another embodiment, Z is-CH-.
In another embodiment, Z is-C (alkyl)-.
In a further embodiment, Z be-C (OH)-.
In another embodiment, Z be-C (O-alkyl)-.
In another embodiment, Z is-C (CF 3)-.
In another embodiment, Z is-N-.
In another embodiment, exist this optional two keys and Z to be-C-.
In one embodiment, n is 0.
In another embodiment, n is 1.
In another embodiment, n is 2.
In one embodiment, p is 0.
In another embodiment, p is 1.
In one embodiment, n and p are 1.
In another embodiment, n be 0 and p be 1.
In another embodiment, n be 2 and p be 1.
In one embodiment, p is 1 and R 2, R 3, R 3a, R 10, R 10aAnd R 11Each is H naturally.
In another embodiment, p is 1 and R 2, R 10, R 10aAnd R 11Each is H naturally.
In another embodiment, p is 1 and R 3, R 3a, R 10, R 10aAnd R 11Each is H naturally.
In one embodiment, Z is-N-; P is 1; And R 2, R 3, R 3a, R 10, R 10aAnd R 11Each is H naturally.
In another embodiment, Z is-N-; P is 1; And R 2, R 10, R 10aAnd R 11Each is H naturally.
In another embodiment, Z is-N-; P is 1; And R 3, R 3a, R 10, R 10aAnd R 11Each is H naturally.
In one embodiment, n and p each naturally 1 and R 2, R 3, R 3a, R 10, R 10aAnd R 11Each is H naturally.
In another embodiment, n and p each naturally 1 and R 2, R 10, R 10aAnd R 11Each is H naturally.
In another embodiment, n and p each naturally 1 and R 3, R 3a, R 10, R 10aAnd R 11Each is H naturally.
In one embodiment, W is-C (R 5) 2-and Z be-N-.
In another embodiment, W is-N (R 12)-and Z be-N-.
In another embodiment, W is-C (R 5) 2-and Z be-CH-.
In another embodiment, W is-N (R 12)-and Z be-CH-.
In further embodiment, W is-CH (NH 2)-,-CH (OH)-or CH (NHSO 2CH 3), and Z is-N-.
In another embodiment, W is-CH (NH 2)-,-CH (OH)-or CH (NHSO 2CH 3) and Z be-CH-.
In yet another embodiment, W is-N (R 12)-be-N-with Z.
In another embodiment, W be-NH-and Z be-N-.
In one embodiment, Ar is a phenyl, and W is-C (R 5) 2-with Z be-N-.
In another embodiment, Ar is a phenyl, and W is-N (R 12)-be-N-with Z.
In another embodiment, Ar is a phenyl, and W is-C (R 5) 2-with Z be-CH-.
In yet another embodiment, Ar is a phenyl, and W is-N (R 12)-be-CH-with Z.
In one embodiment, Ar is a pyridyl, and W is-C (R 5) 2-with Z be-N-.
In another embodiment, Ar is a pyridyl, and W is-N (R 12)-be-N-with Z.
In another embodiment, Ar is a pyridyl, and W is-C (R 5) 2-with Z be-CH-.
In yet another embodiment, Ar is a pyridyl, and W is-N (R 12)-be-CH-with Z.
In specific embodiment, following group
Figure BPA00001171830600351
Be:
Figure BPA00001171830600361
In one embodiment, R 1Be H, methyl, ethyl,
Figure BPA00001171830600362
And following group
Figure BPA00001171830600363
Be:
Figure BPA00001171830600364
In one embodiment, the invention provides compound or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer, the wherein R of formula (I) 1, R 2, R 3, R 3a, R 10, R 10a, R 11, Ar, n, p, M, W, Y and Z are selected independently of one another.
In one embodiment, described diamido thiazole derivatives has formula (IA):
Figure BPA00001171830600371
And pharmacy acceptable salt, solvate, ester, prodrug and steric isomer, wherein
X is-N-or-CH-;
M is-C (O)-or-NHC (O); With
R 1Be H, alkyl, heteroaryl, aryl ,-alkylidene group-aryl or Heterocyclylalkyl.
In one embodiment, this diamido thiazole derivatives has formula (IA) and R 1Be H, methyl, ethyl,
Figure BPA00001171830600372
In another embodiment, this diamido thiazole derivatives has formula (IA) and X is N.
In another embodiment, this diamido thiazole derivatives has formula and X is CH.
In another embodiment, this diamido thiazole derivatives have formula (IA) and M be-C (O)-.
In another embodiment, this diamido thiazole derivatives have formula (IA) and M be-NHC (O)-.
In one embodiment, this diamido thiazole derivatives has formula (IA), and wherein X is-N-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600381
In another embodiment, this diamido thiazole derivatives has formula (IA), and wherein X is-CH-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600382
In one embodiment, this diamido thiazole derivatives has formula (IA), and wherein M is-C (O)-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600391
In another embodiment, this diamido thiazole derivatives has formula (IA), and wherein M is-NHC (O)-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600392
In one embodiment, the invention provides compound or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer, the wherein R of formula (IA) 1, M and X are selected independently of one another.
In one embodiment, this diamido thiazole derivatives has formula (IB):
Figure BPA00001171830600401
With its pharmacy acceptable salt, solvate, ester, prodrug and steric isomer, wherein
X is-N-or-CH-;
M is-C (O)-or-NHC (O)-; With
R 1Be H, alkyl, heteroaryl, aryl ,-alkylidene group-aryl or Heterocyclylalkyl.
In one embodiment, this diamido thiazole derivatives has formula (IB) and R 1Be H, methyl, ethyl,
Figure BPA00001171830600402
In another embodiment, this diamido thiazole derivatives has formula (IB) and X is N.
In another embodiment, this diamido thiazole derivatives has formula (IB) and X is CH.
In yet another embodiment, this diamido thiazole derivatives have formula (IB) and M be-C (O)-.
In another embodiment, this diamido thiazole derivatives have formula (IB) and M be-NHC (O)-.
In one embodiment, this diamido thiazole derivatives has formula (IB), and wherein X is-N-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600411
In another embodiment, this diamido thiazole derivatives has formula (IB), and wherein X is-CH-and R 1Be H, methyl, ethyl,
In one embodiment, this diamido thiazole derivatives has formula (IB), and wherein M is-C (O)-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600421
In another embodiment, this diamido thiazole derivatives has formula (IB), and wherein M is-NHC (O)-and R 1Be H, methyl, ethyl,
Figure BPA00001171830600422
In one embodiment, the invention provides the compound or the pharmacy acceptable salt of formula (IB), solvate, ester, prodrug or its steric isomer, wherein R 1, M and X are selected independently of one another.
In one embodiment, for the compound of formula (I), variable R 1, R 2, R 3, R 3a, R 10, R 10a, R 11, Ar, M, Q, W, Y, Z, n and p are selected independently of one another.
In another embodiment, the compound of formula (I) is purified form.
The nonrestrictive illustrative example of the diamido thiazole derivatives of formula (I) comprises the following compound 1-16 that enumerates:
Compound
Structure
Numbering
Figure BPA00001171830600441
And pharmacy acceptable salt, solvate, ester, prodrug and steric isomer.
Extra non-limiting, the illustrative example of the diamido thiazole derivatives of formula (I) comprises the compound 17-26 that is described as following examples part, and pharmacy acceptable salt, solvate, ester, prodrug and steric isomer.
The method for preparing diamido thiazole derivatives
Can be used for described in the method such as following scheme 1-7 of diamido thiazole derivatives of preparation formula (I).Alternative mechanical approach and similar structures are conspicuous for the organic synthesis those skilled in the art.
Scheme 1 illustrates the method for the compound of preparation formula iv, and the compound of described formula iv can be used as intermediate and is used to prepare wherein Z and is-N (R for-N-and W 12)-the compound of formula (I).
Scheme 1
Figure BPA00001171830600451
X wherein aBe F or Cl, and R 2, R 3, Ar and n such as above-mentioned compound for formula (I) are defined.
Aryl that the nitro of formula i replaces or heteroaryl derivative can be under diisopropyl ethyl amine (DIEA) carry out coupling with the diethylenediamine compound of formula ii by the process of microwave-assisted, obtain passing through link coupled compound i ii.Can use the nitryl group reduction of appropriate means then, obtain the intermediate amine compound of formula iv the compound of formula iii.
Scheme 2 illustrates the method for the compound of preparation formula vii, the compound of described formula vii can be used as intermediate be used to prepare wherein Z for-N-and W for not being-N (R 12)-the compound of formula (I).
Scheme 2
Figure BPA00001171830600452
X wherein aBe F or Cl, and R 2, R 3, W, Ar and n such as above-mentioned compound for formula (I) are defined.
Aryl that the nitro of formula i replaces or heteroaryl derivative can obtain through link coupled compound vi by the cyclammonium coupling of DIEA coupling method described in the scheme 1 and formula v.Can use the nitryl group reduction of appropriate means then, obtain the intermediate amine compound of formula vii the compound of formula vi.
Scheme 3 illustrates the method for the compound of preparation formula x, and the compound of described x can be used as intermediate to be used to prepare wherein Z be carbon and W and is-N (R 12)-the compound of formula (I).
Scheme 3
Figure BPA00001171830600461
Wherein X is Cl, Br or-OTf; M is B (OH) 2, ZnX or SnBu 3And R 2, R 3, Ar and n such as above-mentioned compound for formula (I) are defined.
Aryl that the nitro of formula i replaces or heteroaryl derivative can use the piperidine compounds coupling of catalytic coupling method of Pd (for example, Suzuki coupling, Negishi coupling or Stille coupling) and formula viii, obtain through link coupled compound i x.Can use of the nitryl group reduction of suitable reduction method then, obtain the intermediate amine compound of formula x the compound of formula ix.
Scheme 4 illustrates the method for the compound of preparation formula xiii, and the compound of described formula xiii can be used as intermediate and is used to prepare wherein that Z is that carbon and W are not-N (R 12)-the compound of formula (I).
Scheme 4
Wherein X is-Cl ,-Br or-OTf; M is B (OH) 2, ZnX or SnBu 3And R 2, R 3, W, Ar and n such as above-mentioned compound for formula (I) are defined.
Aryl or heteroaryl derivative that the nitro of formula viii replaces can obtain the compound of formula xiii by the compound coupling of Pd coupling method described in the scheme 3 and formula xii.Can use the nitryl group reduction of appropriate means then, obtain the intermediate amine compound of formula xiv the compound of formula xiii.
Scheme 5 illustrate prepare M wherein for-OC (O)-the method for diamido thiazole derivatives of formula (I).
Scheme 5
Figure BPA00001171830600471
Wherein PG is a carboxyl-protecting group, and R 2, R 3, W, Y, Z and Ar as above define at the compound of formula (I).
2-ethoxycarbonyl-thiazole-4-carboxylic acid's compound of formula xiv can with amine compound (its representation compound iv of formula XV; vii; x or xiii) use 2-(1H-7-azepine benzo triazol-1-yl)-1; 1; 3, there is N in 3-tetramethyl-urea hexafluorophosphate (HATU), and coupling is to provide the intermediate of formula xvi under the situation of N-diisopropylethylamine; it can deprotection and uses known method further refining so that the compound of formula (I) to be provided, wherein M be-OC (O)-.
Scheme 6 illustrate be used to make wherein that M is-NHC (O)-the method for diamido thiazole derivatives of formula (I).
Scheme 6
Figure BPA00001171830600472
R wherein 1, R 2, R 3, W, Y, Z and Ar define as the compound at above-mentioned formula (I).
The carboxylic acid cpd of formula xvii can with formula NH 2R 2Amine use the condition coupling be described in the scheme 5 that the compound of formula xviii is provided, it is equivalent to the compound of formula (I), wherein M be-NHC (O)-.
Scheme 7 illustrates the method for the diamido thiazole derivatives that is used for manufacturing formula (I), wherein M be-C (O)-.
Scheme 7
Figure BPA00001171830600481
R wherein 1, R 2, R 3, W, Y, Z and Ar such as above compound at formula (I) define.
The carboxylic acid cpd of formula xvii can provide the acid chloride compounds of xix with the thionyl chloride reaction.The compound of formula xix then can with formula R 1The reaction of the compound of Li provides the compound of formula xx, and it is equivalent to the compound of formula (I), wherein M be-C (O)-.
The guidance and the known method in the organic synthesis field that use embodiment 1-7 to provide, those skilled in the art will obviously know the carboxylic moiety of the compound of control type xvii how so that the compound of corresponding formula (I) is provided, wherein M is S (O) 2-or-NHS (O) 2-.
Embodiment
General method
Commercially available solvent, reagent and intermediate use with the state of receiving.Not commercially available reagent and intermediate prepare in mode hereinafter described. 1H NMR spectrum is to go up at Varian AS-400 (400MHz) to obtain, and with distance Me 4The ppm of Si downfield gives a report, and wherein proton number, multiplicity and coupling constant (representing with Hz) are to be instructed in bracket.Providing under the LC/MS data conditions, analyzing below the use: Applied Biosystems API-100 mass spectrograph and Shimadzu SCL-10A LC post: Altech platinum C18,3 microns, 33mmx7mm ID; Gradient flow quantity: 0min-10%CH 3CN, 5min-95%CH 3CN, 7min-95%CH 3CN, 7.5min-10%CH 3CN, 9min-stops.Use Agilent Technologies LC/MSD SL or 1100 serial LC/MSD mass spectrographs to obtain the MS data.By the final compound of preparation type LC purifying, use Varian Pursuit XRs C18 10 μ m 250x21.2mm posts, and the mixture of eluents of mobile phase A and B.Mobile phase A consists of the H that (is composed of) contains 0.1%TFA 2O, Mobile phase B consists of CH 3CN (95%)/H 2O (5%)/TFA (0.1%).The mixture of mobile phase A and B at room temperature passes through the pillar wash-out with the flow velocity of 20mL/min.The purity of all final isolated compound is to use the mixture of eluents of Higgins Haisil HL C 18 5 μ m 150x4.6mm posts and mobile phase A and B to detect by LCMS, and wherein mobile phase A consists of the H that contains 0.1%TFA 2O, Mobile phase B consists of CH 3CN (95%)/H 2O (5%)/TFA (0.1%).Under 60 ℃ temperature with the flow velocity wash-out pillar of 3mL/min.Midbody compound characterizes by LCMS, uses the mixture of eluents of Higgins Haisil HL C185 μ m 50x4.6mm pillar and mobile phase A and B, and wherein mobile phase A consists of the H that contains 0.1%TFA 2O, Mobile phase B consists of CH 3CN (95%)/H 2O (5%)/TFA (0.1%).Under 60 ℃ column temperature with the flow velocity wash-out pillar of 3mL/min.
Embodiment 1
Preparation compound 1A
Figure BPA00001171830600491
4-chloro-3-nitropyridine (5.04 grams, 31.8 mmole), 1-piperazinecarboxylic acid tertiary butyl ester (7.16 grams, 38.4 mmoles) and N, the solution of N-diisopropylethylamine (17.0 milliliters, 97.6 mmoles) in anhydrous dioxane (50 milliliters) was 95 ℃ of heating 14 hours.After the cooling, reaction is handled with methyl alcohol (5 milliliters) dilution with the 7N ammonia in the methyl alcohol (25 milliliters, 175 mmoles).The suspensoid stir about of gained 5 minutes filters and the solid washed with dichloromethane then, produce transparent, garnet filtrate, it is concentrated provides yellow-orange solids (11.4 restrain).Solid provides compound 1A by purified by flash chromatography with 2%-3% methylene chloride-methanol wash-out, is orange/yellow solid (8.96 grams, 91% yield).
Embodiment 2
Preparation compound 2A
Compound 1A (9.42 gram, 29.0 mmoles) is suspended in 1: 1 ethyl acetate-methyl alcohol of 90 milliliters and adds 10% palladium on carbon (1.21 grams, 1.14 mmoles) under nitrogen.Suspensoid on the Parr wobbler 45psi hydrogenation 6 hours.Reaction mixture filtration over celite, and diatomite methanol wash.Concentrated filtrate provides the yellow-green colour oil of viscosity under vacuum condition, and it is dissolved in the methylene dichloride again and flows down concentrated at nitrogen.Resistates vacuum-drying provides compound 2A, is brown solid (7.62 grams, 94% yield).
Embodiment 3
Preparation compound 3A
Figure BPA00001171830600502
The mixture of 3-martonite acid (16.4 grams, 98.0 mmoles) in anhydrous dioxane (90 milliliters) handled with thio acylamino ethyl oxalate (13.1 grams, 98.2 mmoles).Reaction flask immerses and to be preheating to 50 ℃ oil bath, this solution homogeneous fully in several minutes.50 ℃ of stirring reactions 1.5 hours, concentrate at 50 ℃ then dry yellow solid is provided.Crude product is dissolved in saturated sodium bicarbonate (150ml) and water (150ml).This solution extracts and discarded organic extraction with ethyl acetate (6X400 milliliter).The waterbearing stratum uses 21 milliliters dense HCl acidified aqueous solution to pH2 then, produces to bolarious change and the heavy precipitation of formation.This suspensoid uses ethyl acetate (5X500ml) to extract then.These extracts are merged, and use dried over sodium sulfate, and filtering and concentrating in a vacuum provides compound 3A, are reddish-brown solid (14.4 grams, 73%).
Embodiment 4
Preparation compound 4A
Figure BPA00001171830600511
Compound 3A (0.300 gram, 1.49 solution mmole) is dissolved in N, dinethylformamide (2.98 milliliters) is used PYBOP (0.854 gram, 1.64 mmoles) then successively, (0.492 milliliter of 4-methylmorpholine, 4.47 mmole) and 1,2,3,4-tetrahydroquinoline (0.218 milliliter, 1.64 mmoles) is handled.Spend the night and add 15 milliliters water, waterbearing stratum CH then at leisure in stirring at room reaction 2Cl 2(3 * 15 milliliters) extract.With the saturated NaHCO of organic layer that merges 3Aqueous solution solution (25 milliliters) and salt solution (25 milliliters) washing.Organic layer Na 2SO 4Drying is filtered and is concentrated.Product uses the gradient of 0-40% ethyl acetate-hexane by purified by flash chromatography, and compound 4A is provided (0.121 gram, 26% yield).
Method that use is set forth in embodiment 4 and alternative suitable reactant prepare following midbody compound:
Figure BPA00001171830600521
Figure BPA00001171830600531
Embodiment 5
Preparation compound 17
Figure BPA00001171830600532
The solution of compound 4A (0.121 gram, 0.382 mmole) in methyl alcohol (1.4 milliliters) and tetrahydrofuran (THF) (1.4 milliliters) is dropwise handled with the solution of 2M aqueous NaOH (0.210 milliliter, 0.420 mmole).Envrionment temperature stirring reaction 15 hours.This solution is concentrated the yellow solid that 0.123g is provided with vacuum-drying.Resistates is dissolved in N, in the dinethylformamide (0.79 milliliter), uses PYBOP (0.268 gram, 0.515 mmole) then successively, 4-methylmorpholine (0.087 milliliter, 0.793 mmole) and compound 50 (0.121 gram, 0.436 mmole) are handled.Envrionment temperature stirring reaction 3 days.The throw out of this solution with water (60ml) dilution and filtration gained, water (2X5 milliliter) washing and vacuum-drying provide compound 17 (0.084 gram, 38% yield).
Method that use is set forth in embodiment 5 and alternative suitable reactant prepare following midbody compound:
Figure BPA00001171830600541
Figure BPA00001171830600551
Embodiment 6
Preparation compound 10
Figure BPA00001171830600552
Solution in compound 20 (0.019 gram, the 0.032 mmole) methylene dichloride (5 milliliters) is handled with trifluoroacetic acid (1.50 milliliters, 19.5 mmoles).This solution is in stirring at room 1 hour and concentrated.Be dissolved in resistates in 1.5 milliliters of 2: 1 formic acid-water and the solution of gained uses reversed-phase HPLC purifying on Waters 25mm PrepLC post, the operation gradient is 5 to 20% water-acetonitrile.Obtain the formate of compound 10 thus, be colourless oil (0.0147 gram, 86% yield). 1H?NMR(CD 3OD)δ8.95(br?s，1H)，8.71(s，1H)，8.45(br?s，1H)，7.95(s，1H)，7.70(d，J＝9.2Hz，1H)，7.49(t，J＝8.0Hz，1H)，7.26(s，1H)，7.10(d，J＝8.0Hz，1H)，3.29-3.45(m，8H)；MH +＝493。
Use above elaboration method and alternative suitable reactant in embodiment 6, be prepared as the following compound of the formula (I) of its formate:
Figure BPA00001171830600571
Embodiment 7
Preparation compound 15
Figure BPA00001171830600581
Compound 17 (0.093 gram, 0.15 mmole) is dissolved in the solution of methylene dichloride (5 milliliters), handles with trifluoroacetic acid (1.50 milliliters, 19.5 mmoles).This solution is in stirring at room 1 hour and concentrated.Product is used in 5%7N NH in the MeOH-methylene dichloride by the preparative chromatography purifying 3Wash-out provides compound 15, is yellow solid (0.043 gram, 63% yield). 1H?NMR(CD 3OD)δ9.14(s，1H)，8.28(d，J＝5.6Hz，1H)，8.09(s，1H)，7.25(d，J＝7.6Hz，1H)，7.19(d，J＝5.6Hz，1H)，7.08(t，J＝7.6Hz，1H)，6.98(t，J＝7.6Hz，1H)，6.80-6.90(br?s，1H)，3.98(t，J＝6.4Hz，2H)，3.07(br?s，8H)，2.92(t，J＝6.4Hz，2H)，2.06-2.13(m，，2H)；MH +＝449。
Use the above method of in embodiment 7, setting forth and substitute suitable reactant, prepare compound with following formula (I):
Figure BPA00001171830600591
Embodiment 8
CHK1 SPA test
The reorganization His-CHK1 that this in vitro tests utilization is expressed in baculovirus expression system as enzyme source and based on the biotinylated peptide of CDC25C as substrate (vitamin H-RSGLYRSP SMPENLNRPR).
Material and reagent:
1) CDC25C Ser 216C-end biotinylation peptide substrates (25mg) is stored in-20 ℃, is entrusted synthetic by Research Genetics: vitamin H-RSGLYRSP SMPENLNRPR 2595.4MW
2) His-CHK1 In House lot P976,235 μ g/mL are stored in-80 ℃.
3) D-PBS (not containing CaCl and MgCl): GIBCO, Cat.# 14190-144
4) SPA pearl: Amersham, the Cat.#SPQ0032:500mg/ bottle
The working concentration for preparing 50mg/mL for the SPA globule interpolation 10mL D-PBS of 500mg.Be stored in 4 ℃.In 2 weeks, use after the hydration.
5) have 96 holes white microplate: the Packard of bonded GF/B strainer, Cat.# 6005177
6) top seal-96 hole adhesive film: Perkin Elmer, Cat.# 6005185
7) 96 holes do not have bonded white polystyrene board: Corning, and Cat.# 6005177
8)MgCl 2：Sigma，Cat.#?M-8266
9)DTT：Promega，Cat.#?V3155
10) ATP is stored in 4 ℃: Sigma, Cat.# A-5394
11)γ 33P-ATP，1000-3000?Ci/mMol：Amersham，Cat.#?AH9968
12)NaCl：Fisher?Scientific，Cat.#?BP358-212
13)H 3PO 4?85％?Fisher，Cat.#?A242-500
14)Tris-HCL?pH?8.0：Bio-Whittaker，Cat.#?16-015V
15) staurosporine, 100 μ g:CALBIOCHEM, Cat.# 569397
16) Hypure cell cultures level water, 500mL:HyClone, Cat.# SH30529.02
Reaction mixture:
1) kinase buffer liquid: 50mM Tris pH 8.0; 10mM MgCl 21mM DTT
2) His-CHK1, In House Lot P976, MW～30KDa is stored in-80 ℃.
Need 6nM with obtain～5, the positive control of 000CPM.For 1 plate (100 reactions): 235 μ g/mL (the 7.83 μ M) stock solution of 8 μ L is diluted in the 2mL kinase buffer liquid.Obtain the mixture of 31nM like this.Add 20 μ L/ holes.Obtain the end reaction concentration of 6nM like this.
3) the biotinylated peptide of CDC25C.
With CDC25C dilution for the stock solution of 1mg/mL (385 μ M) and be stored in-20 ℃.For 1 plate (100 reactions): the 1mg/mL peptide stock solution of 10 μ L is diluted in the 2mL kinase buffer liquid.Obtain the mixture of 1.925 μ M like this.Add 20 μ L/ reaction.Obtain the end reaction concentration of 385nM like this.
4) ATP mixture.
For 1 plate (100 reactions): 1mM ATP (cold) stock solution of 10 μ L and the fresh P33-ATP of 2 μ L (20 μ Ci) are diluted in the 5mL kinase buffer liquid.Obtain 2 μ MATP (cold) solution like this; Add 50 μ L/ holes to start reaction.Final volume is 100 μ L/ reactions, and end reaction concentration is 1 μ M ATP (cold) and 0.2 μ Ci/ reaction thus.
5) stop bath:
Interpolation for 1 plate: to 10mL lavation buffer solution 2 (2M NaCl 1%H 3PO 4) the middle 1mLSPA globule slurries (50mg) that add; Add 100 μ L/ holes
6) lavation buffer solution 1:2M NaCl
7) lavation buffer solution 2:2M NaCl, 1%H 3PO 4
Test operation:
Figure BPA00001171830600611
*The total reaction volume of test. *The end reaction volume of reaction end (after adding stop bath).
1) test compound is diluted concentration for needing in water/10%DMSO, obtaining like this in reaction is 1% final DMSO concentration.Be assigned in the suitable hole with 10 μ L/ reaction.Heliotropism control wells (CHK1+CDC25C+ATP) and negative control hole (only containing CHK1+ATP) add the 10%DMSO of 10 μ L.
2) enzyme is thawed on ice-enzyme is diluted to suitable concentration (referring to reaction mixture) also for distributing 20 μ L in each hole in kinase buffer liquid.
3) biotinylated substrate is thawed on ice and in kinase buffer liquid the dilution (referring to reaction mixture).Except negative control hole, add 20 μ L/ holes.As an alternative, negative control wells is added the kinase buffer liquid of 20 μ L.
4) ATP (cold) and P33-ATP are diluted in kinase buffer liquid (referring to reaction mixture).Add 50 μ L/ holes to start reaction.
5) make and be reflected at room temperature and carried out 2 hours.
6) by adding SPA globule/stop bath (referring to reaction mixture) termination reaction of 100 μ L, be incubated and collect after 15 minutes.
7) blank Packard GF/B filter plate is placed vacuum apparatus (Packard plate collector), suck 200mL water and pass through with wetting this system.
8) take out this blank plate and put into Packard GF/B filter plate.
9) the suction reaction is by this filter plate.
10) washing: 200mL/ washing; With 2M NaCl washing 1 time; Use 2M NaCl/1%H 3PO 4Wash 1 time.
11) made filter plate dry 15 minutes.
12) top seal-A adhesive film is placed the filter plate top.
13) filter plate is counted in Top Count
Be provided with: data pattern: CPM
Active nucleus: manual SPA:P33
Scintillator: Liq/plast
Energy region: low
IC 50 Measure: draw dose response curve according to the inhibition data that the 8-point serial dilutions from the a-type double of inhibitor compound generates.Compound concentrations is drawn to the % kinase activity, and this % kinase activity is by calculating divided by the CPM of unprocessed sample with the CPM of treated sample.In order to generate IC 50Value fits to dose response curve the standard sigmoid curve then, derives IC by nonlinear regression analysis 50Value.
Used this experimental examination selected heterocyclic ether of the present invention or sulfide derivative obtains the IC of the about 10 μ M of about 1nM- 50Value.
Embodiment 9
The CDK2 test
Baculovirus makes up:By PCR with cyclin E be cloned into pVL1393 (Pharmingen, La Jolla, California) in, add 5 histidine residues at N-terminal, to allow purifying on the nickel resin.Expressed proteins is approximately 45kDa.By PCR CDK2 is cloned among the pVL1393, adds hemoglutinin epitope label (YDVPDYAS) at C-terminal.The expressed proteins size is approximately 34kDa.
Enzyme produces:With identical infection multiplicity (MOI=5) with the recombinant baculovirus coinfection of express cell cyclin E and CDK2 in the SF9 cell, carried out 48 hours.By with centrifugal 10 minutes collecting cells of 1000RPM, make pellet molten born of the same parents on ice 30 minutes with molten born of the same parents' damping fluid of 5 times of pellet volumes then, this damping fluid contains 50mM Tris pH 8.0,150mMNaCl, 1%NP40,1mM DTT and proteinase inhibitor (Roche Diagnostics GmbH, Mannheim, Germany).Make lysate rotation 10 minutes with 15000RPM, keep supernatant liquor.(Qiagen GmbH, Germany) middle washing is 3 times at molten born of the same parents' damping fluid with 5mL nickel bead (for 1 liter SF9 cell).Imidazoles is added in the baculovirus supernatant liquor, and making ultimate density is 20mM, down is incubated 45 minutes with nickel bead at 4 ℃ then.With the molten born of the same parents' buffer solution eluted protein matter that contains the 250mM imidazoles.Make the elutriant dialysed overnight in 2 liters of kinase buffer liquid, this kinase buffer liquid contains 50mM Tris pH 8.0,1mM DTT, 10mM MgCl 2, 100 μ M sodium orthovanadates and 20% glycerine.The enzyme portioning is preserved.
Used this experimental examination selected heterocyclic ether of the present invention or sulfide derivative obtains the IC of the about 50 μ M of about 10 μ M- 50Value.
Embodiment 10
Cell in vitro cyclin E/CDK2 kinase assay
Cyclin E/CDK2 kinase assay can as described belowly be carried out in low protein bound 96-orifice plate (Corning Inc, Corning, New York).
Enzyme is diluted to the ultimate density of 50 μ g/mL in kinase buffer liquid, this kinase buffer liquid contains 50mM Tris pH 8.0,10mM MgCl 2, 1mM DTT and 0.1mM sodium orthovanadate.The substrate that is used for these reactions is that the biotinylated peptide that derives from histone h1 (derives from Amersham, UK).Substrate is thawed on ice, in kinase buffer liquid, be diluted to 2 μ M again.Make test compound in 10%DMSO, be diluted to the concentration of expectation.For each kinase reaction, the 50 μ g/mL enzyme solution (enzyme of 1 μ g) of 20 μ L and the 2 μ M substrate solutions of 20 μ l are mixed, merge in each hole of test usefulness with the diluted compound of 10 μ L then.2 μ M ATP by adding 50 μ L and the 33P-ATP of 0.1 μ Ci (derive from Amersham, UK) start kinase reaction.Make and be reflected at room temperature and carried out 1 hour.By adding 200 μ L stop buffers and keeping coming termination reaction in 15 minutes, this damping fluid contains 0.1%Triton X-100,1mM ATP, and the SPA globule that 5mM EDTA and 5mg/mL streptavidin apply (derives from Amersham, UK).Use Filtermate common collector (Packard/Perkin Elmer Life Sciences.) then, the SPA globule is collected in the 96-hole GF/B filter plate (Packard/Perkin Elmer Life Sciences).By washing globule 2 times, wash globule 2 times with the 2M NaCl that contains 1% phosphoric acid then, to remove non-specific signal with 2M NaCl.Can use for example TopCount 96 hole liquid scintillation counters (from Packard/Perkin Elmer Life Sciences) mensuration emission signal then.
IC 50Measure: the inhibition data drafting dose response curve that generates according to 8-point serial dilutions from the a-type double of inhibitor compound.Compound concentrations is drawn to the % kinase activity, and this % kinase activity is by calculating divided by the CPM of unprocessed sample with the CPM of treated sample.In order to generate IC 50Value fits to dose response curve the standard sigmoid curve then, can derive IC by nonlinear regression analysis 50Value.
Embodiment 11
The MEK1 kinase assay
Baculovirus infection by the Hi-Five cell makes total length active phosphorus acidifying MEK1 be expressed as 6X histidine tagged protein (His 6-MEK1), this Hi-Five cell baculovirus coinfection of the Raf-1 that expresses unlabelled constitutive activity.Then with several milligrams active His 6-MEK1 then carries out the gel filtration chromatography purifying with Ni-NTA affinity chromatography purifying.The lysine mutation that will have in subdomain II becomes arginic total length Muridae catalysis non-activity ERK2KR as substrate.Make ERK2KR be expressed as the fusion rotein of biotinylated 6X Histidine and Trx mark from the carrier pET32aRC IPTG-inductive BL21D3 intestinal bacteria, and by Ni-NTA affinity chromatography purifying, then by Mono Q ion-exchange chromatogram purification.Under 25 ℃, in 96 orifice plates, carry out kinase reaction 15min in duplicate, every hole 33 μ L, and this kinase reaction consist of 20nM His 6-MEK1,2 μ M ERK2KR, 2 μ M ATP, 10 μ Ci/ μ L[γ- 33P]-ATP, 10mM MgCl 2, 0.01% β-octyl glucoside, 1mM DTT, 20mM HEPES pH 7.5,3%DMSO and scope be the test compound that 20 μ M reduce to 0.08nM.Stop kinase reaction by the 1.5%o-phosphoric acid that adds 30 μ L, transfection and is incubated 5 minutes to allow the ERK2KR combination in the MilliporeMultiscreen-PH plate.React and estimate non-specific activity according to wherein before adding enzyme, the 1.5%o-phosphoric acid of 30 μ L being added to pre-deactivation in each hole.By with the vacuum filtration of 0.75%o-phosphoric acid with terminated plate washing 3 times, then with 100% washing with alcohol 2 times, and dry air.Add the flicker mixture of 50 μ L for each hole, re-use Wallac Microbeta 1450 JET scintillometer and detect and be attached among the ERK2KR 33P.Use ActivityBase computed in software percent inhibition, IC 50With the Hill slope value.
Used this experimental examination selected heterocyclic ether of the present invention or sulfide derivative obtains the IC of the about 100 μ M of about 10nM- 50Value.
Embodiment 12
Be used for the general operation of MEK1TdF test
In the PCR plate of white 96-hole, make 1 μ M albumen and the compound of testing the micro-molar concentration (being generally 1-50 μ M) in the damping fluids (25mMHEPES, pH 7.4,300mM NaCl, 1mM DTT, 2%DMSO, Sypro Orange 5x) at 20 μ l.By transparent strip plate is sealed, place again thermal cycler (Chromo4, BioRad) on.Monitoring fluorescence intensity with per 0.5 ℃ of increment from 25 ℃ to 95 ℃ between melting period.Data are outputed to the excel table, obtain TdF Kd value through the custom curve fitting algorithm again.The error that all TdF Kd values have is limited to～and 50%, reason is the uncertainty due to the bonded heat content changes.
Used this experimental examination selected heterocyclic ether of the present invention or sulfide derivative obtains the K of the about 100 μ M of about 1 μ M- dValue.
Embodiment 13
Be used for the general operation of MEK1 Delfia enzymic activity test
Use has wherein been measured independent inhibition % and the dose response curve (IC of compound based on the restraining effect of the enzyme test mensuration compound of DELFIA (Perkin-Elmer) 50Measure).Activatory recombinant human MEK1 (5 nmole ultimate density) pre-incubation that will be in damping fluid 10 minutes, comprise biotin labeled reorganization MEK1 substrate ERK (1 little ultimate density of rubbing) by interpolation afterwards and start reaction, wherein said damping fluid comprises Hepes, magnesium chloride, dithiothreitol (DTT) and ATP (2 micromole's ultimate density).Make to be reflected at 20 ℃ of operations 60 minutes, transfer to the last stopped reaction of the ROCHE streptavidin microplate (Perkin-Elmer #11734776001) that comprises DELFIA test damping fluid (Perkin-Elmer #4002-0010) by reacting aliquot sample afterwards.Under agitation after room temperature was in conjunction with 1 hour, plate with DELFIA lavation buffer solution (Perkin-Elmer#4010-0010) washing, is added the DELFIA test damping fluid (Perkin Elmer #AD0040) that comprises the Tyrosine O-phosphate specific antibody and is incubated 1 hour as mentioned above for plate subsequently.After washing for the second time, plate is developed by adding Perkin-Elmer enhancing solution (#4001-0010) and under agitation being incubated 10 minutes subsequently.Read to read on the plate device europium fluorescence at Victor 1420 fluorescent plates.Shine into row to recently measuring inhibition % and IC by test and reaction pair with inclusion compound 50
Used this experimental examination selected heterocyclic ether of the present invention or sulfide derivative obtains the IC of the about 100 μ M of about 10nM- 50Value.
Embodiment 14
External aurora TdF test
Aurora A test
Aurora body A kinase assay is to carry out in lower protein bonded 384-orifice plate (Corning company).Whole reagent are thawed on ice.Test compound is diluted to the concentration of expectation in 100%DMSO.Each reactant consists of 8nM enzyme (aurora body A, Upstate catalogue #14-511), 100nM Tamra-PKAtide (Molecular Devices, 5TAMRA-GRTGRRNSICOOH), 25 μ M ATP (Roche), 1mM DTT (Pierce) and kinase buffer liquid (10mM Tris, 10mM MgCl2,0.01% polysorbas20).The diluted compound of each reaction and 1 μ L that will contain 14 μ L of TAMRA-PKAtide, ATP, DTT and kinase buffer liquid merges.By adding the enzyme of 5 μ L dilution, kinase reaction is begun.Reaction was at room temperature carried out 2 hours.(1: 400 bead stops reaction at incremental (94.7% buffer A: 5.3% buffer B) 1X damping fluid, among the 24mM NaCl) by adding 60 μ L IMAP globules.Behind other 2 hours, use Analyst AD (Molecular devices) to measure fluorescence polarization.
Aurora B test
Aurora A kinase assay is carried out in lower protein bonded 384-orifice plate (Corning company).Whole reagent are thawed on ice.Test compound is diluted to the concentration of expectation in 100%DMSO.Each reactant comprises 26nM enzyme (aurora body B, Invitrogen cat#pv3970), 100nM Tamra-PKAtide (Molecular Devices, 5TAMRA-GRTGRRNSICOOH), 50 μ M ATP (Roche), 1mM DTT (Pierce) and kinase buffer liquid (10mM Tris, 10mMMgCl2,0.01% polysorbas20).The compound through dilution of each reaction and 1 μ L that will contain 14 μ L of TAMRA-PKAtide, ATP, DTT and kinase buffer liquid merges.By adding the enzyme of 5 μ L dilution, kinase reaction is begun.Reaction was at room temperature carried out 2 hours.(1: 400 bead stops reaction at incremental (94.7% buffer A: 5.3% buffer B) 1X damping fluid, among the 24mM NaCl) by adding 60 μ L IMAP globules.Behind other 2 hours, use Analyst AD (Molecular devices) to measure fluorescence polarization.
IC 50Measure
Draw dose response curve from the inhibition data that the 8-point serial dilution of bipartite test compound generates.Compound concentrations is mapped to kinase activity, and this kinase activity calculates by the fluorescence polarization number of degrees.In order to generate IC 50Value fits to dose response curve standard S shape curve then, and derives IC by nonlinear regression analysis 50Value.
Used this experimental examination selected heterocyclic ether of the present invention or sulfide derivative obtains the K of the about 100 μ M of about 1nM- dValue.
The purposes of diamido thiazole derivatives
Described diamido thiazole derivatives can be used for treating or preventing patient's the patient's condition.
Can include but not limited at United States Patent (USP) 6,413 by other disease or the illness that at least a diamido thiazole derivatives that gives significant quantity is treated, those disclosed in 974, described patent is merged in this paper as a reference.
Treatment or preventing cardiovascular disease
Described diamido thiazole derivatives can be used for treating or preventing patient's cardiovascular disorder.
Therefore, in one embodiment, the invention provides the method for treatment patient's cardiovascular disorder, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
Can use the illustrative example of the cardiovascular disorder of the inventive method treatment or prevention to include but not limited to atherosclerosis, congestive heart failure, arrhythmia, myocardial infarction, auricular fibrillation, auricular flutter, cyclical shock, left ventricular hypertrophy, ventricular tachycardia, supraventricular tachycardia, coronary artery disease, stenocardia, infective endocarditis, noninfectious endocarditis, myocardosis, peripheral arterial disease, Raynaud disease, venous thrombosis, aortic stenosis, mitral stenosis, pulmonic stenosis and tricuspid stenosis.
In one embodiment, described cardiovascular disorder is an atherosclerosis.
In another embodiment, described cardiovascular disorder is congestive heart failure.
In another embodiment, described cardiovascular disorder is a coronary artery disease.
Treatment or prevention CNS illness
Described diamido thiazole derivatives can be used for treating or preventing patient's central nervous system (CNS) illness.
Therefore, in one embodiment, the invention provides the method for treatment patient's CNS illness, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
Can use the illustrative example of the CNS illness of the inventive method treatment or prevention to include but not limited to the central nervous system activities deficiency, central nervous system activities is excessive, neurodegenerative disease, alzheimer's disease, amyotrophic lateral sclerosis (ALS), Creutzfeldt-Jakob disease, Huntington Chorea, multiple sclerosis, Lay dimension body illness, the spasm illness, figure thunder syndromes, Parkinson's disease, Pick's disease, prion disease or schizophrenia, epilepsy, migraine, anxiety disorder, bipolar disorder, dysthymia disorders, not enough hyperactivity hyperkinesia illness (ADHD) of attention and dementia.
In one embodiment, described CNS illness is an alzheimer's disease.
In another embodiment, described CNS illness is Parkinson's disease.
In another embodiment, described CNS illness is ALS.
Treatment or prophylaxis of viral infections
Described diamido thiazole derivatives can be used for treating or preventing patient's virus infection.
Therefore, in one embodiment, the invention provides the method for treatment patient's virus infection, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
Can use the illustrative example of the virus infection of the inventive method treatment or prevention to include but not limited to HIV, human papillomavirus (HPV), simplexvirus, poxvirus, Epstein-Barr virus, sindbis alphavirus and adenovirus.
In one embodiment, described virus infection is HIV.
In another embodiment, described virus infection is HPV.
Treatment or prevention fungi infestation
Described diamido thiazole derivatives can be used for treating or preventing patient's fungi infestation.
Therefore, in one embodiment, the invention provides the method for treatment patient's fungi infestation, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
Can use the illustrative example of the fungi infestation of the inventive method treatment or prevention to include but not limited to aspergillosis, blastomycosis, moniliosis, coccidioides immitis, torulosis, histoplasmosis, opportunistic fungus (comprising yeast and mould), mucormycosis, mycetoma, Brazilian blastomycosis and sporotrichosis.
In one embodiment, described fungi infestation is moniliosis.
Treatment or prevention and protein kinase activity diseases associated
Described diamido thiazole derivatives can be inhibitor, modulator or the conditioning agent of protein kinase, and can be used for treating or prevent the patient with the protein kinase activity diseases associated.
Therefore, in one embodiment, the invention provides the treatment patient and the method protein kinase activity diseases associated, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
Can use the illustrative example with the protein kinase activity diseases associated of the inventive method treatment or prevention to include but not limited to cell cycle protein dependent kinase (CDK), such as CDK1, CDK2, CDK3, CDK4, CDK5, CDK6 and CDK7, CDK8; Aurora kinase, such as aurora-A, aurora-B and aurora-C; Mitogen activated protein kinase (MAPK/ERK); Glycogen Synthase kinase 3 (GSK3 β); The c-Met kinases is such as c-Met; The Pim-1 kinases; Checkpoint kinase such as Chk1 and Chk2; Tyrosylprotein kinase (comprises for example EGFR (HER1), HER2 such as the HER subfamily, HER3 and HER4), the Regular Insulin subfamily (for example comprises INS-R, IGF-IR, IR, and IR-R), the PDGF subfamily (for example comprises PDGF-α and beta receptor, CSFIR, c-kit and FLK-II), FLK family (for example comprises kinases insert structure domain receptor (KDR), fetus liver kinases-1 (FLK-1), fetus liver kinases-4 (FLK-4) and fms-sample Tyrosylprotein kinase-1 (flt-1)); Non-receptor protein tyrosine kinase, for example, LCK, Src, Frk, Btk, Csk, Abl, Zap70, Fes/Fps, Fak, Jak, Ack, and LIMK; And growth factor receptor tyrosine kinase, such as VEGF-R2, FGF-R, TEK, Akt kinases etc.
In one embodiment, the invention provides the method that in the patient who needs is arranged, suppresses one or more checkpoint kinases, comprise at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer of the patient being treated significant quantity.
In another embodiment, the invention provides in the patient who needs is arranged treatment and one or more checkpoint kinase diseases associated or delay the method for its progress, comprise at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer of the patient being treated significant quantity.
In another embodiment, the invention provides the method for one or more and checkpoint kinase diseases associated of treatment, comprise that the patient to this treatment of needs gives at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer, and at least a other carcinostatic agent, the volume production of wherein said at least a diamido thiazole derivatives and at least a carcinostatic agent is given birth to therapeutic action.
In another embodiment, the invention provides in the patient who needs is arranged treatment and one or more checkpoint kinase diseases associated or delay the method for its progress, comprise the pharmaceutical composition of the patient being treated significant quantity, described pharmaceutical composition comprises at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer with at least a pharmaceutically acceptable carrier combinations.
The checkpoint kinase that be suppressed in one embodiment,, modulate or regulate is Chk1.The checkpoint kinase that be suppressed in another embodiment,, modulate or regulate is Chk2.
In one embodiment, the invention provides the method that in the patient who needs is arranged, suppresses one or more Tyrosylprotein kinases, comprise at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer of the patient being treated significant quantity.
In another embodiment, the invention provides in the patient who needs is arranged treatment and one or more Tyrosylprotein kinase diseases associated or delay the method for its progress, comprise at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer of the patient being treated significant quantity.
In another embodiment, the invention provides the method for one or more and Tyrosylprotein kinase diseases associated of treatment, comprise that the patient to this treatment of needs gives at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer, and at least a other carcinostatic agent, the volume production of wherein said at least a diamido thiazole derivatives and at least a carcinostatic agent is given birth to therapeutic action.
In another embodiment, the invention provides in the patient who needs is arranged treatment and one or more Tyrosylprotein kinase diseases associated or delay the method for its progress, comprise the pharmaceutical composition of the patient being treated significant quantity, described pharmaceutical composition comprises at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer with at least a pharmaceutically acceptable carrier combinations.
In specific embodiment, the described Tyrosylprotein kinase that be suppressed, modulate or regulate is VEGFR (VEGF-R2), EGFR, HER2, SRC, JAK or TEK, or its combination.
In one embodiment, the invention provides the kinase whose method of one or more Pim-1 that suppresses to have the patient who needs, comprise at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer of the patient being treated significant quantity.
In another embodiment, the invention provides in the patient who needs is arranged treatment and one or more Pim-1 kinases diseases associated or delay the method for its progress, comprise at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer for the treatment of significant quantity.
In another embodiment, the invention provides the method for one or more and Pim-1 kinases diseases associated of treatment, comprise that the patient to this treatment of needs gives at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer, and at least a other carcinostatic agent, the volume production of wherein said at least a diamido thiazole derivatives and at least a carcinostatic agent is given birth to therapeutic action.
In another embodiment, the invention provides in the patient who needs is arranged treatment and one or more Pim-1 kinases diseases associated or delay the method for its progress, comprise the pharmaceutical composition of the patient being treated significant quantity, described pharmaceutical composition comprises at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer with at least a pharmaceutically acceptable carrier combinations.
In one embodiment, the invention provides the method for one or more and an aurora kinase diseases associated of treatment, comprise that the patient to this treatment of needs gives at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer, and at least a other carcinostatic agent, the volume production of wherein said at least a diamido thiazole derivatives and at least a carcinostatic agent is given birth to therapeutic action.
In another embodiment, the invention provides in the patient who needs is arranged treatment and one or more aurora kinase diseases associated or delay the method for its progress, comprise the pharmaceutical composition of the patient being treated significant quantity, described pharmaceutical composition comprises at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer with at least a pharmaceutically acceptable carrier combinations.
In one embodiment, the invention provides the method for one or more and cell cycle protein dependent kinase diseases associated of treatment, comprise that the patient to this treatment of needs gives a certain amount of first compound, with a certain amount of at least a second compound, wherein first compound is diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer, second compound is the carcinostatic agent that is different from described diamido thiazole derivatives, and wherein the volume production of first compound and second compound is given birth to therapeutic action.
Described diamido thiazole derivatives can also be used to suppress the kinase whose oncogene of coded protein.The limiting examples of this oncogene comprises C-Met.
Treatment or prevention proliferative disorders
Described diamido thiazole derivatives can be used for treating or preventing patient's proliferative disorders.Therefore, in one embodiment, the invention provides the method for treatment patient's proliferative disorders, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
Can use the illustrative example of the proliferative disorders of the inventive method treatment or prevention to include but not limited to cancer, atherosclerosis, the benign prostate hyperplasia, familial adenhomatosis polyposis, neurofibromatosis, atherosclerosis, pulmonary fibrosis, sacroiliitis, psoriatic, glomerulonephritis, restenosis after angioplasty or the vascular operation, hypertrophic cicatrix forms, inflammatory bowel, transplant rejection, endotoxin shock, idiopathic pulmonary fibrosis, scleroderma and liver cirrhosis.
Induce or suppress apoptosis
Described diamido thiazole derivatives can be used for inducing or suppressing patient's apoptosis.
Therefore, in one embodiment, the invention provides the apoptotic method of inducing or suppressing the patient, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
In multiple human diseases, the apoptosis response is unusual, as the apoptosis conditioning agent, described diamido thiazole derivatives can be used for treating cancer, virus infection, the AIDS development of prevention HIV infected individuals, autoimmune disorder (includes but not limited to systemic lupus erythematous, lupus erythematosus, the glomerulonephritis of autoimmunization mediation, rheumatoid arthritis, psoriatic, inflammatory bowel, and autoimmune diabetes), neurodegenerative disorders (includes but not limited to alzheimer's disease, AIDS is relevant dull-witted, Parkinson's disease, amyotrophic lateral sclerosis, retinitis pigmentosa, Duchenne-Arandisease and cerebellar degeneration), spinal cord heteroplasia syndrome, aplastic anemia, with myocardial infarction, the ischemia injury that apoplexy is relevant with reperfusion injury, arrhythmia, atherosclerosis, hepatic diseases toxin-induced or that alcohol is correlated with, blood disease (including but not limited to chronic anaemia disease and aplastic anemia), flesh osseous system degenerative disease (including but not limited to osteoporosis and sacroiliitis), the aspirin sensitive sinusitis paranasal sinusitis, cystic fibrosis, multiple sclerosis, kidney disease and cancer pain.
Treatment or preventing cancer
Described diamido thiazole derivatives can be used for treating or preventing patient's cancer.
Therefore, in one embodiment, the invention provides treatment patient's method for cancer, comprise one or more diamido thiazole derivatives that the patient given significant quantity.
The illustrative example of the cancer that can use the inventive method treatment or prevent includes but not limited to the cancer of bladder, breast, colon, rectum, kidney, liver, lung (comprising small cell lung cancer, nonsmall-cell lung cancer, mesothelioma and carcinoma gigantocellulare), head and neck, oesophagus, gall-bladder, ovary, pancreas, stomach, uterine cervix, Tiroidina, prostate gland and skin (comprising squamous cell carcinoma and melanoma); Lymphoid hematopoiesis tumour (includes but not limited to leukemia, such as acute lymphoblastic leukemia, chronic lymphocytic leukemia or acute lymphocytoblast leukemia; Lymphoma is such as B cell lymphoma, t cell lymphoma, Hodgkin lymphoma, non-Hodgkin lymphoma, hair cell lymphoma, lymphoma mantle cell, myelomatosis or Burkitt lymphoma); The cancer of bright reason not; The hematopoiesis tumour of marrow system includes but not limited to acute and chronic graininess leukemia, spinal cord heteroplasia syndrome and promyelocytic leukemia; Between the tumour of matter origin, include but not limited to fibrosarcoma and rhabdosarcoma; The tumour of maincenter and peripheral nervous system includes but not limited to cerebral tumor, such as astrocytoma, neuroblastoma, neurospongioma (such as glioblastoma multiforme) or schwannoma; With other tumour, comprise spermocytoma, malignant teratoma, osteosarcoma, pigment xeroderma, angling spinose cell's knurl, Tiroidina trichocarcinoma and Kaposi sarcoma.Described diamido thiazole derivatives can be used for treating primary and/or metastatic cancer.
Described diamido thiazole derivatives can also be used for the chemoprophylaxis of cancer.Chemoprophylaxis is defined as by blocking-up initiation mutagenesis incident or by blocking the precellular progress of deterioration that has sustained an injury or suppressing the development that tumor recurrence suppresses invasive cancer.
Described diamido thiazole derivatives can also be used to suppress tumor-blood-vessel growth and transfer.
The cancer of in one embodiment, treating or preventing is selected from: mammary cancer, colorectal carcinoma, lung cancer, prostate cancer, ovarian cancer, carcinoma of the pancreas, skin carcinoma, leukemia and lymphoma.
In another embodiment, the cancer for the treatment of or preventing is selected from: mammary cancer, colorectal carcinoma, lung cancer and prostate cancer.
The cancer of in one embodiment, treating or preventing is a mammary cancer.
The cancer of in another embodiment, treating or preventing is a lung cancer.
The cancer of in another embodiment, treating or preventing is a colorectal carcinoma.
In another embodiment, the cancer for the treatment of or preventing is a prostate cancer.
In another embodiment, the cancer for the treatment of or preventing is a leukemia.
In another embodiment, the cancer for the treatment of or preventing is a lymphoma.
In one embodiment, the cancer of treatment or prevention is a solid tumor.
In another embodiment, the cancer of treatment or prevention is the cancer of blood or lymph.
In one embodiment, the cancer of treatment or prevention is a primary carcinoma.
In another embodiment, the cancer of treatment or prevention is a metastatic cancer.
In another embodiment, the patient since primary carcinoma and metastatic cancer receive treatment.
Combined therapy
In one embodiment, the invention provides the method for treatment patient's the patient's condition, described method comprises one or more diamido thiazole derivatives of patient's administration or its pharmacy acceptable salt, solvate, ester or prodrug, and the other therapeutical agent of at least a non-diamido thiazole derivatives, wherein dosage is effective for the treatment or the prevention patient's condition altogether.
The other therapeutical agent that can be used for the inventive method includes but not limited to carcinostatic agent, can be used for treating the medicine of cardiovascular disorder, two or more any combination of the medicine that can be used for treating the CNS illness, antiviral drug, antifungal drug, antiproliferative agents, anti-alopecia medicine, antiphlogiston, the medicine that can be used for treating the protein kinase associated conditions, ischemia resisting medicine or these medicines.
In another embodiment, other therapeutical agent is the medicine that can be used for reducing any possible side effect of diamido thiazole derivatives.This possible side effect includes but not limited to feel sick, the pain of vomiting, headache, heating, lethargic sleep, sore muscle, diarrhoea, whole body pain and injection site.
When the patient to this administration of needs gives combined therapy, the various therapeutical agents of described combined therapy or comprise that one or more compositions of therapeutical agent can be with any order administration, such as for example, order administration, parallel administration, co-administered, administration simultaneously etc.The amount of the various promoting agents in this combined therapy can be different amounts (various dose) or identical amount (same dose).
In one embodiment, shown in the administration when other therapeutical agent is brought into play its prevention or therapeutic action of one or more diamido thiazole derivatives, perhaps vice versa.
In another embodiment, described one or more diamido thiazole derivatives and other therapeutical agent are with at the normally used dosed administration during as the independent therapy that is used for the treatment of the patient's condition with this reagent.
In another embodiment, the dosage of described one or more diamido thiazole derivatives and other therapeutical agent is lower than at the normally used dosage during as the independent therapy that is used for the treatment of the patient's condition with this reagent.
In another embodiment, described one or more diamido thiazole derivatives and other therapeutical agent work synergistically, and dosage is lower than at the normally used dosage during as the independent therapy that is used for the treatment of the patient's condition with this reagent.
In one embodiment, described one or more diamido thiazole derivatives and other therapeutical agent are present in the same composition.In one embodiment, this composition is suitable for oral administration.In another embodiment, this composition is suitable for intravenous administration.
Described one or more diamido thiazole derivatives and other therapeutical agent can the addition ground or are worked synergistically.Collaborative combination can allow to use than one or more reagent of low dosage and/or reduce the administration frequency of one or more reagent of combined therapy.Can the reducing the toxicity of treatment and not reduce the effectiveness of treatment of one or more reagent than low dosage or low administration frequency.
In one embodiment, the administration of one or more diamido thiazole derivatives and other therapeutical agent can suppress the patient's condition to one or more the resistance in these medicines.
In one embodiment, described other therapeutical agent uses with its known treatment effective dose.In another embodiment, described other therapeutical agent is with its dosage use of prescription usually.In another embodiment, the using dosage of described other therapeutical agent is lower than its dosage or its known treatment effective dose of prescription usually.
Be used for the treatment of or prevent the dosage and the dosage regimen of other reagent that in combined therapy of the present invention, uses of the patient's condition can decide and consider dosage and the dosage regimen of ratifying in the package insert by clinicist on the scene; Patient's age, sex and general health situation; The type of virus infection or relative disease or illness and severity.When combination medicine-feeding, be used for the treatment of diamido thiazole derivatives and the administration simultaneously of other reagent or the order administration of the above-mentioned disease or the patient's condition.For example particularly useful when this each component in combination gives with different dosed administration timetables, a component is administered once every day, and another component was administered once in per six hours; Perhaps particularly useful in the different situation of described composition, for example one is tablet, and another is a capsule.Therefore comprise that the cartridge bag of independent formulation is favourable.
Usually, described one or more diamido thiazole derivatives and the other total per daily dose of therapeutical agent as the combined therapy administration time can be the about 2000mg/ of about 0.1-days, but depend on target, patient and the route of administration of treatment, and it will inevitably change to some extent.In one embodiment, described dosage is the about 100mg/ of about 0.2-days, as single dosage or as 2-4 dosed administration that separates.In another embodiment, described dosage is the about 500mg/ of about 1-days, as single dosage or as 2-4 dosed administration that separates.In another embodiment, described dosage is the about 200mg/ of about 1-days, as single dosage or as 2-4 dosed administration that separates.In another embodiment, described dosage is the about 100mg/ of about 1-days, as single dosage or as 2-4 dosed administration that separates.In a further embodiment, described dosage is the about 50mg/ of about 1-days, as single dosage or as 2-4 dosed administration that separates.In another embodiment, described dosage is the about 20mg/ of about 1-days, as single dosage or as 2-4 dosed administration that separates.
The combined therapy that is used for the treatment of cancer
Compound of the present invention can also be used for the anti-cancer therapies independent with one or more (such as, operation, radiotherapy, biology therapy are (for example, the anti-cancer vaccine therapy)) and/or at least a administration combined (give together or give successively) that is different from the other carcinostatic agent of diamido thiazole derivatives, in order to treatment or prevention patient's cancer with any order.Compound of the present invention can be present in the same dosage device with other carcinostatic agent or be present in the independent dosage device.
Be applicable to that the limiting examples with the other carcinostatic agent (being also referred to as antineoplastic agent) of The compounds of this invention and usefulness comprises cytostatic agent, cytotoxic agents (such as such as but not limited to DNA agent interfering (for example cis-platinum or Dx)); Taxanes (for example taxotere (taxotere), taxol); Topoisomerase II inhibitor (for example Etoposide or teniposide); Topoisomerase I inhibitor (for example Rinotecan (or CPT-11), Kan Sida (camptostar) or topotecan); Tubulin agent interfering (for example taxol, docetaxel (docetaxel) or esperamicin (epothilones)); Hormone drug (for example tamoxifen); Thymidylic acid (thymidilate) synthetase inhibitors (for example 5 FU 5 fluorouracil); Antimetabolite (for example Rheumatrex (methoxtrexate)); Alkylating agent (Temozolomide (TEMODAR for example TM, derive from Schering-Plough Corporation, Kenilworth, New Jersey), endoxan); Farnesyl protein transferase inhibitors (for example, SARASAR TM(4-[2-[4-[(11R)-3,10-two bromo-8-chloro-6,11-dihydro-5H-benzo [5,6] encircle heptan [1,2-b] pyridine-11-yl]-piperidino]-the 2-oxoethyl]-the 1-piperidyl urea, perhaps derive from Schering-Plough Corporation, Kenilworth, the SCH 66336 of New Jersey), tipifarnib ( Or R115777, derive from Janssen Pharmaceuticals), L778,123 (farnesyl protein transferase inhibitors derives from Merck ﹠amp; Company, Whitehouse Station, New Jersey), BMS 214662 (farnesyl protein transferase inhibitors derives from Bristol-Myers Squibb Pharmaceuticals, Princeton, NewJersey); (for example, Iressa (derives from Astra ZenecaPharmaceuticals, England), antibody (for example, C225), the imatinib mesylate of Te Luokai (EGFR kinase inhibitor), EGFR to signal transduction inhibitor TM(the C-abl kinase inhibitor derives from NovartisPharmaceuticals, East Hanover, New Jersey); Interferons is such as for example, because of wound (deriving from Schering-Plough Corporation), Pegylation-Yin wound (deriving from Schering-Plough Corporation); The hormonotherapy combined method; The aromatase enzyme combined method; Ara-C, AC and gemcitabine.
Other useful other carcinostatic agent include but not limited to uracil mustard, mustargen, ifosfamide, L-PAM, Chlorambucil, pipobroman (Pipobroman), triethylene melamine, ara-C, AC, Rimonophenazine ( Derive from Genzyme Oncology, Cambridge, Massachusetts), CldAdo (
Figure BPA00001171830600771
Derive from Janssen-Cilag Ltd.), Ba Fei enemy Ke Lin (aphidicolon), Rituximab (rituxan) (deriving from Genentech/BiogenIdec), Sutent (
Figure BPA00001171830600772
Derive from Pfizer), Dasatinib (or BMS-354825, derive from Bristol-Myers Squibb), for pricking his shore (deriving from Aventis Pharma), Sml1, fludarabine (deriving from Trigan Oncology Associates), pentostatin (deriving from BC CancerAgency), complete flat (triapine) (deriving from Vion Pharmaceuticals), ground degree this (didox) (deriving from Bioseeker Group), full Mi Duosi (trimidox) (deriving from ALS Therapy DevelopmentFoundation), amidox, 3-AP (3-aminopyridine-2-formaldehyde thiosemicarbazone), MDL-101,731 ((E)-2 '-deoxidation-2 '-(fluorine methylene radical) cytidine) and gemcitabines.
Other useful other carcinostatic agent includes but not limited to triethylene sulfo-phosphamidon, busulfan, carmustine, lomustine, streptozocin, dacarbazine, fluorodeoxyuridine, cytosine arabinoside, Ismipur, 6-sulfenyl guanine, fludarabine phosphate, oxaliplatin, formyl tetrahydrofolic acid, oxaliplatin (ELOXATIN TMDerive from Sanofi-SynthelaboPharmaceuticals, France), pentostatin, vinealeucoblastine(VLB), vincristine(VCR), vindesine, bleomycin, dactinomycin, daunorubicin, Dx, epirubicin, idarubicin, Plicamycin, deoxycoformycin, Mitomycin-C, the altheine enzyme, teniposide, the female alcohol of 17 alpha-acetylenes, stilboestrol, testosterone, prednisone, FL, dromostanolone propionate, testolactone, the acetate megestrol, medrat, methyltestosterone, Prednisolone Acetate, triamcinolone, Chlortrianisoestrol, hydroxyprogesterone, aminoglutethimide, estramustine, the Zytron acetic ester, Leuprolide, Drogenil, toremifene, goserelin, cis-platinum, carboplatin, oxaliplatin, A Luo platinum (Aroplatin), hydroxyurea, amsacrine, Procarbazine, mitotane (Mitotane), mitoxantrone, L-tetramisole, nvelbine, Anastrozole, letrozole, capecitabine, raloxifene, droloxifene, Hexamethylmelamine, A Wasiting (Avastin), Trastuzumab (Herceptin), hectogram sand (Bexxar), ten thousand jade-like stones (Velcade), Ze Waling (Zevalin), white arsenic (Trisenox), xeloda (Xeloda), vinorelbine, porphines nurse (Profimer), Erbitux, liposome (Liposomal), thio-tepa, altretamine, L-PAM, Herceptin, letrozole, fulvestrant, Exemestane, fulvestrant, ifosfamide, Rituximab, C225 and Kan Pasi (Campath).
In one embodiment, other carcinostatic agent is selected from cytostatic agent, cis-platinum, Dx, taxotere (taxotere), taxol, Etoposide, irinotecan, Kan Sida (camptostar), topotecan, taxol, docetaxel, esperamicin, tamoxifen, 5-fluor-uracil, methotrexate, Temozolomide, endoxan, SCH 66336, R115777, L778,123, BMS 214662, Iressa (Iressa), Te Luokai (Tarceva), the antibody of EGFR, imatinib mesylate (Gleevec), because of wound (intron), ara-C, Zorubicin, endoxan, gemcitabine, Uramustine, mustargen, ifosfamide, melphalan, Chlorambucil, pipobroman, Tretamine, triethylene sulfo-phosphamidon, busulfan, carmustine, lomustine, streptozocin, Dacarbazine, floxuridine, cytosine arabinoside, Ismipur, 6-thioguanine, fludarabine phosphate, pentostatin, vinealeucoblastine(VLB), vincristine(VCR), vindesine, bleomycin, dactinomycin, daunorubicin, Dx, epirubicin, idarubicin, Plicamycin, deoxycoformycin, Mitomycin-C, the L-Asparaginase, teniposide, the female alcohol of 17 alpha-acetylenes, stilboestrol, testosterone, prednisone, FL, dromostanolone propionate, Testolactone, the acetate megestrol, methylprednisolone, Synrotabs, Prednisolone Acetate, triamcinolone, Chlortrianisoestrol, hydroxyprogesterone, aminoglutethimide, estramustine, Amen, Leuprolide, flutamide, toremifene, goserelin, carboplatin, hydroxyurea, amsacrine, Procarbazine, mitotane, mitoxantrone, LEVAMISOLE HCL, nvelbine, Anastrozole, letrozole, capecitabine, raloxifene, droloxifene, Hexamethylmelamine, A Wasiting, Trastuzumab, hectogram sand, ten thousand jade-like stones, Ze Waling, white arsenic, xeloda, vinorelbine, porphines nurse (Profimer), Erbitux, liposome, plug is for group, altretamine, melphalan, Herceptin, letrozole, fulvestrant, Exemestane, ifosfamide, sharp appropriate Xidan is anti-, C225, many west (Doxil, liposomal doxorubicin), Ang Take (Ontak), Supreme Being's baud (Deposyt), Mai Luotage (Mylotarg), the bank Paasche, celecoxib, comfortable (Sutent), A Fadabei Bo Ting (Aranesp), excellent Bao Jin (Neupogen), Xin Sita (Neulasta), Kevin Si (Kepivance), SU11248, and PTK787.
In one embodiment, other carcinostatic agent is based on the medicine of platinum, for example cis-platinum, carboplatin or oxaliplatin.
In another embodiment, other carcinostatic agent is an alkylating agent.
In another embodiment, other carcinostatic agent is a vinca alkaloids, such as vincristine(VCR) or vinealeucoblastine(VLB).
In another embodiment, other carcinostatic agent is the topoisomerase I inhibitor.
In another embodiment, other carcinostatic agent is the topoisomerase II inhibitor.
In another embodiment, other carcinostatic agent is a metabolic antagonist.
In another embodiment, other carcinostatic agent is the spindle body poison.
In another embodiment, other carcinostatic agent is an antitumor antibiotics.
If be formulated as fixed dosage, use compound of the present invention in the described in this article dosage range of this combination product and in the dosage range of its approval, use other forms of pharmacologically active agents or therapy.For example, have been found that CDC2 inhibitor olomoucine (olomucine) is when cell death inducing and known cytotoxic agents work synergistically (J.Cell Sci., (1995) 108, 2897.When combination preparation is inappropriate, diamido thiazole derivatives also can with the administration of known carcinostatic agent or cytotoxic agents order.Without limits, diamido thiazole derivatives can administration before or after known carcinostatic agent or cytotoxic agents aspect order of administration in the present invention.For example, the cytotoxic activity of cyclin dependant protein kinase flavopiridol is subjected to the influence of the order of administration of carcinostatic agent.CancerResearch，(1997) 57，3375。This technology is in those skilled in the art and attending doctor's skill.
Therefore, in one aspect, the present invention includes treatment patient's method for cancer, comprise giving a certain amount of at least a diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer and one or more other anticancer therapy patterns that wherein the volume production of diamido thiazole derivatives/other treatment pattern is given birth to the desired therapeutic effect to the patient.In one embodiment, described at least a diamido thiazole derivatives and one or more other treatment patterns work synergistically.In another embodiment, described at least a diamido thiazole derivatives works with one or more other with treating the pattern addition.
In one embodiment, other treatment pattern is operation.
In another embodiment, other treatment pattern is a radiotherapy.
In another embodiment, other treatment pattern is the biology therapy, such as hormonotherapy or anti-cancer vaccine therapy.
The pharmacological property of The compounds of this invention can confirm by many pharmacology tests.Compound of the present invention and their salt, solvate, ester or prodrug have been carried out the exemplary pharmacology test of the following stated herein.
Composition and administration
The invention still further relates to pharmaceutical composition, described pharmaceutical composition comprises pharmacy acceptable salt, solvate, ester or prodrug and at least a pharmaceutically acceptable carrier of at least a diamido thiazole derivatives or described compound.
For from compound pharmaceutical composition of the present invention, inert, pharmaceutically acceptable carrier can be solid or liquid.But the solid form prepared product comprises powder, tablet discrete particles, capsule, cachet and suppository.Powder and tablet can comprise the activeconstituents of about 5-about 95%.The solid carrier that is fit to is as known in the art, for example, and magnesiumcarbonate, Magnesium Stearate, talcum, sugar or lactose.Can be with tablet, powder, cachet and capsule as the solid dosage that is suitable for oral administration.The example of pharmaceutically acceptable carrier and produce various method for compositions can be A.Gennaro (editor), Remington ' s Pharmaceutical Sciences, the 18th edition, (1990), Mack Publishing Co., Easton finds among the Pennsylvania.
The liquid form prepared product comprises solution, suspension and emulsion.As an example, can mention the water or the water-propylene glycol solution that are used for non-enteron aisle injection, or be that oral liquid, suspension and emulsion add sweeting agent and opalizer.The liquid form prepared product can also comprise the solution that is used for intranasal administration.
The aerosol prepared product that is suitable for sucking can comprise the solid of solution and powder type, and it can make up with pharmaceutically acceptable carrier (such as inert pressurized gas, for example nitrogen).
Also be included in the solid form prepared product that is intended to be converted into the liquid form prepared product that is used for oral or parenterai administration before being about to use.This liquid form prepared product comprises solution, suspension and emulsion.
But compound of the present invention can also be a transdermal delivery.Transdermal composition can be the form of creme, washing lotion, aerosol and/or emulsion, and can as in this area for habitual it being included in matrix type or the depot percutaneous plaster of this purpose.
Compound of the present invention can also subcutaneous delivery.
Preferably, with compound oral administration or intravenous administration or intrathecal drug delivery or with some suitable array mode administration.
Preferably, pharmaceutical preparation is a unit dosage form.In this form, prepared product is subdivided into the dosage unit of the suitable size that comprises an amount of active ingredient, and described appropriate amount for example is to be used to realize the purpose significant quantity expected.
The amount of the active compound in the dosage unit prepared product can or be regulated from the about 500mg variation of about 0.001mg-.In one embodiment, the amount of the active compound in the dosage unit prepared product is the about 250mg of about 0.01mg-.In another embodiment, the amount of the active compound in the dosage unit prepared product is the about 100mg of about 0.1mg-.In another embodiment, the amount of the active compound in the dosage unit prepared product is the about 100mg of about 1.0mg-.In another embodiment, the amount of the active compound in the dosage unit prepared product is the about 50mg of about 1.0mg-.In another embodiment, the amount of the active compound in the dosage unit prepared product is the about 25mg of about 1.0mg-.
Employed actual dose can depend on the severity of patient's the needs and the patient's condition of being treated and change.Suitable dosage regimen for particular case fixes in those skilled in the art's the limit of power really.For convenience's sake, can be as required with total per daily dose separately and in one day as a plurality of part administrations.
Modulate the dosage and the administration frequency of compound of the present invention and/or its pharmacy acceptable salt according to clinicist's on the scene judgement, and consider factors such as severity such as age, the patient's condition and the patient's size and the symptom for the treatment of.The typical recommended dosage regimen of oral administration can be the diamido thiazole derivatives of about 0.01mg/ days-Yue 2000mg/ days.In one embodiment, the per daily dose dosage regimen of oral administration is about 1mg/ days-1000mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 1mg/ days-500mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 100mg/ days-500mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 1mg/ days-250mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 100mg/ days-250mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 1mg/ days-100mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 50mg/ days-100mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 1mg/ days-50mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 25mg/ days-50mg/ days.In another embodiment, the per daily dose dosage regimen of oral administration is about 1mg/ days-25mg/ days.Described per daily dose can perhaps can be divided into 2-4 dosage that separates with independent dosed administration.
Cartridge bag
In one aspect, the invention provides cartridge bag, described cartridge bag comprises one or more diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester or the prodrug of significant quantity, and pharmaceutically acceptable carrier.
In one aspect of the method, the invention provides cartridge bag, described cartridge bag comprises a certain amount of one or more diamido thiazole derivatives or its pharmacy acceptable salt, solvate, ester or prodrug, and a certain amount of at least a above-mentioned other therapeutical agent of enumerating, the amount of wherein said combination is effective for treatment or prevention patient's the patient's condition.
In each component of combined therapy scheme in a more than composition during administration, they can be provided in comprise in the cartridge bag of independent packing, described independent packing comprises one or more containers, one of them container is included in one or more diamido thiazole derivatives in the pharmaceutically acceptable carrier, second independent container is included in the other therapeutical agent in the pharmaceutically acceptable carrier, the amount of the active ingredient of each composition make described combination be treatment effectively.
In one aspect of the method, the invention provides cartridge bag, described cartridge bag comprises a certain amount of at least a diamido thiazole derivatives or described compound pharmacy acceptable salt, solvate, ester or prodrug, and a certain amount of at least a anti-cancer therapies and/or the above-mentioned other carcinostatic agent of enumerating, the volume production of wherein said two or more compositions is given birth to the desired therapeutic effect.
Scope of the present invention is not limited to disclosed specific embodiment among the embodiment, and it is intended to illustrate aspects more of the present invention, and the embodiment of any function equivalent all within the scope of the invention.In fact, except shown and described herein of the present invention various improvement those are conspicuous for those skilled in the relevant art, and be intended to also fall in the scope of claims.
This paper has quoted many reference, and the whole open of described reference all incorporated into this paper as a reference in full.

Claims (60)

1. the compound that has following formula:
With its pharmacy acceptable salt, solvate, ester, prodrug and steric isomer, wherein dotted line is represented that choose wantonly and other key, and wherein:
M is-C (O)-,-C (S)-,-S (O)-,-S (O) 2-,-NHS (O) 2-,-OC (O)-or-NHC (O)-;
Q is:
Figure FPA00001171830500012
R 1Be H, alkyl, thiazolinyl, alkynyl ,-(alkylidene group) m-aryl ,-(alkylidene group) m-cycloalkyl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl or-(alkylidene group) m-heterocycloalkenyl, wherein any aryl, cycloalkyl, heteroaryl, Heterocyclylalkyl or heterocycloalkenyl group can be randomly replaced by 3 substituting groups at the most on ring carbon atom or theheterocyclic nitrogen atom, described substituting group can be identical or different and be selected from halo, alkyl, thiazolinyl, alkynyl, haloalkyl, hydroxyalkyl ,-OR 6,-(alkylidene group) m-N (R 6) 2,-C (O) OR 6,-NHC (O) R 6,-C (O) N (R 6) 2,-S (O) 2R 7,-CN ,-OH-,-NO 2,-(alkylidene group) m-aryl ,-(alkylidene group) m-cycloalkyl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl and-(alkylidene group) m-heterocycloalkenyl; Wherein any aryl or heteroaryl substituting group group can be randomly replaced by maximum 5 substituting groups, and described substituting group can be identical or different and be selected from halo ,-OH, alkyl ,-C (O) OR 6,-N (R 6) 2,-NHC (O) R 6,-C (O) N (R 6) 2,-S (O) 2R 7,-CN ,-OH ,-NO 2And-the O-alkyl; Wherein any aryl, cycloalkyl, heteroaryl, Heterocyclylalkyl or heterocycloalkenyl group can randomly condense in aryl, cycloalkyl, heteroaryl, Heterocyclylalkyl or heterocycloalkenyl group;
R 2Each appearance be H independently, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O) R 6Or-(alkylidene group) m-N (R 6) 2, or R 2Form carbonyl group altogether with its ring carbon atom that is connected;
R 3Be H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2, or R 3And R 3aForm the cycloalkyl or the heterocycloalkyl of carbonyl group or volution altogether with the same carbon atom that it is connected separately;
R 3aBe H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2
R 5Each appearance be H independently, alkyl ,-(alkylidene group) m-aryl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl ,-(alkylidene group) m-N (R 6) 2,-(alkylidene group) m-OH ,-(alkylidene group) m-NHC (O) R 6, hydroxyalkyl, haloalkyl ,-C (O) R 6,-C (O) OR 6,-C (O)-(alkylidene group) m-N (R 6) 2,-(alkylidene group) m-NHC (O) R 6,-NHC (O) OR 6Or-NHS (O) 2R 7
R 6Each appearance be H independently, alkyl, haloalkyl, cycloalkyl, aryl, Heterocyclylalkyl or heteroaryl;
R 7Each appearance be H independently, alkyl, aryl, cycloalkyl or haloalkyl;
R 8Be H, alkyl ,-OH ,-O-alkyl or haloalkyl;
R 10Be H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O) R 6Or-(alkylidene group) m-N (R 6) 2, or R 10And R 10aForm the cycloalkyl or the heterocycloalkyl of carbonyl group or volution altogether with the same carbon atom that it is connected separately;
R 10aBe H, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2
R 11Each appearance be H independently, alkyl, haloalkyl, hydroxyalkyl ,-(alkylidene group) m-C (O) N (R 6) 2,-(alkylidene group) m-NHC (O)-R 6Or-(alkylidene group) m-N (R 6) 2, or R 11Form carbonyl group altogether with its ring carbon atom that is connected;
R 12Each appearance be H independently, alkyl ,-(alkylidene group) m-aryl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl ,-S (O) 2R 7, haloalkyl, hydroxyalkyl ,-C (O) R 6Or-C (O) OR 6
Ar is arylidene or inferior heteroaryl, wherein said arylidene or inferior heteroaryl connect by any two its adjacent ring carbon atoms, and wherein said arylidene or inferior heteroaryl group can randomly be replaced by maximum 4 substituting groups, described substituting group can be identical or different and be independently selected from halo, alkyl, alkoxyl group, aryloxy ,-NH 2,-NH-alkyl ,-N (alkyl) 2,-SR 6,-S (O) R 7,-S (O) 2R 7,-C (O) R 6,-C (O) OR 6,-C (O) N (R 6) 2,-NHC (O) R 6, haloalkyl ,-CN and NO 2, make when Ar is the tetrahydrochysene naphthylidene R 2And R 3Not hydrogen separately;
W is-N (R 12) 2-,-S-,-O-or-C (R 5) 2-, wherein be-C (R at W 5) 2-time, two R 5The same carbon atom that group is connected with them can form the cycloalkyl or the heterocycloalkyl of volution altogether, wherein this volution group can randomly be replaced by maximum 4 groups, described group can be identical or different and be selected from halo, alkyl, thiazolinyl, alkynyl, haloalkyl, hydroxyalkyl ,-OR 6,-(alkylidene group) m-N (R 6) 2,-C (O) OR 6,-NHC (O) R 6,-C (O) N (R 6) 2,-S (O) 2R 7,-CN ,-OH ,-NO 2,-(alkylidene group) m-aryl ,-(alkylidene group) m-cycloalkyl ,-(alkylidene group) m-heteroaryl ,-(alkylidene group) m-Heterocyclylalkyl and-(alkylidene group) m-heterocycloalkenyl;
Y is H, halo, alkyl or-CN;
Described optional when not existing with other key, Z is-C (R 8)-or-N-; And described optional when existing with other key, Z is-C-;
Each appearance of m is 0 or 1 independently;
N is the integer of 0-2; With
P is 0 or 1.
2. the compound of claim 1, wherein M be-C (O)-.
3. the compound of claim 1, wherein M be-NHC (O)-.
4. the compound of claim 1, wherein R 1Be H, alkyl, heteroaryl, aryl ,-alkylidene group-aryl or Heterocyclylalkyl.
5. the compound of claim 4, wherein R 1Be H, methyl, ethyl,
Figure FPA00001171830500031
6. the compound of claim 1, wherein Y is H.
7. the compound of claim 1, wherein n and p each naturally 1.
8. the compound of claim 7, wherein R 2, R 3, R 3aR 10, R 10aAnd R 11Respectively naturally-H.
9. the compound of claim 1, wherein Z is that N and W are-N (R 12)-.
10. the compound of claim 9, wherein W is NH.
11. the compound of claim 1, wherein Ar is:
Figure FPA00001171830500041
12. the compound of claim 1, wherein Ar is
Figure FPA00001171830500042
13. the compound of claim 11, wherein Z be-N-and W be-N (R 12)-.
14. the compound of claim 12, wherein Z be-N-and W be-N (R 12)-.
15. the compound of claim 1, wherein group
Figure FPA00001171830500043
Be:
Figure FPA00001171830500044
16. the compound of claim 15, wherein R 1Be H, methyl, ethyl,
17. the compound of claim 1, wherein Q is:
18. the compound of claim 1, wherein Q is:
Figure FPA00001171830500053
19. the compound of claim 1 has following formula:
Figure FPA00001171830500061
With its pharmacy acceptable salt, solvate, ester, prodrug and steric isomer, wherein
M is-C (O)-or-NHC (O)-;
X is-CH-or-N-; With
R 1Be H, alkyl, heteroaryl, aryl ,-alkylidene group-aryl or Heterocyclylalkyl.
20. the compound of claim 19, wherein M be-C (O)-.
21. the compound of claim 19, wherein M be-NHC (O)-.
22. the compound of claim 19, wherein X is-CH-.
23. the compound of claim 19, wherein X is-N-.
24. the compound of claim 19, wherein R 1Be H, methyl, ethyl,
25. the compound of claim 1 has following formula:
Figure FPA00001171830500071
With its pharmacy acceptable salt, solvate, ester, prodrug and steric isomer, wherein
M is-C (O)-or-NHC (O)-;
X is-CH-or-N-; With
R 1Be H, alkyl, heteroaryl, aryl ,-alkylidene group-aryl or Heterocyclylalkyl.
26. the compound of claim 25, wherein M be-C (O)-.
27. the compound of claim 25, wherein M be-NHC (O)-.
28. the compound of claim 25, wherein X is-CH-.
29. the compound of claim 25, wherein X is-N-.
30. the compound of claim 25, wherein R 1Be H, methyl, ethyl,
Figure FPA00001171830500072
31. have the compound that is numbered the structure of 1-16 in the above-mentioned specification sheets, or its pharmacy acceptable salt, solvate, ester, prodrug or steric isomer.
32. the compound of claim 1, it is purified form.
33. composition comprises compound or its pharmacy acceptable salt, solvate, ester, prodrug or the steric isomer of at least a claim 1 of significant quantity and pharmaceutically acceptable carrier.
34. the composition of claim 33 comprises at least a other carcinostatic agent in addition, wherein said other carcinostatic agent is different from the compound of claim 1.
35. the composition of claim 34, wherein said at least a other carcinostatic agent is selected from cytostatic agent, cis-platinum, Dx, taxotere, taxol, Etoposide, irinotecan, Kan Sida, topotecan, taxol, docetaxel, esperamicin, tamoxifen, 5-fluor-uracil, methotrexate, Temozolomide, endoxan, SCH 66336, R115777, L778,123, BMS 214662, Iressa, Te Luokai, the antibody of EGFR, imatinib mesylate, because of wound, ara-C, Zorubicin, endoxan, gemcitabine, Uramustine, mustargen, ifosfamide, melphalan, Chlorambucil, pipobroman, Tretamine, triethylene sulfo-phosphamidon, busulfan, carmustine, lomustine, streptozocin, Dacarbazine, floxuridine, cytosine arabinoside, Ismipur, 6-thioguanine, fludarabine phosphate, pentostatin, vinealeucoblastine(VLB), vincristine(VCR), vindesine, bleomycin, dactinomycin, daunorubicin, Dx, epirubicin, idarubicin, Plicamycin, deoxycoformycin, Mitomycin-C, the L-Asparaginase, teniposide, the female alcohol of 17 alpha-acetylenes, stilboestrol, testosterone, prednisone, FL, dromostanolone propionate, Testolactone, the acetate megestrol, methylprednisolone, Synrotabs, Prednisolone Acetate, triamcinolone, Chlortrianisoestrol, hydroxyprogesterone, aminoglutethimide, estramustine, Amen, Leuprolide, flutamide, toremifene, goserelin, carboplatin, hydroxyurea, amsacrine, Procarbazine, mitotane, mitoxantrone, LEVAMISOLE HCL, nvelbine, Anastrozole, letrozole, capecitabine, Lei Luoshafen, Du Luoshafen, Hexamethylmelamine, A Wasiting, Trastuzumab, hectogram sand, ten thousand jade-like stones, Ze Waling, white arsenic, xeloda, vinorelbine, the porphines nurse, Erbitux, liposome, plug is for group, altretamine, melphalan, Herceptin, letrozole, fulvestrant, Exemestane, ifosfamide, sharp appropriate Xidan is anti-, C225, how western, Ang Take, Supreme Being's baud, Mai Luotage, the bank Paasche, celecoxib, comfortable, A Fadabei Bo Ting, excellent Bao Jin, Xin Sita, Kevin Si, SU11248, and PTK787.
36. treat the patient and the method cell cycle protein dependent kinase diseases associated, comprise the compound that the patient is given at least a claim 1 of significant quantity.
37. the method for claim 36, wherein said cell cycle protein dependent kinase are CDK1.
38. the method for claim 36, wherein said cell cycle protein dependent kinase are CDK2.
39. treat the patient and the method checkpoint kinase diseases associated, comprise the compound that the patient is given at least a claim 1 of significant quantity.
40. the method for claim 39, wherein said checkpoint kinase are Chk1.
41. the method for claim 39, wherein said checkpoint kinase are Chk2.
42. treat the patient and the method aurora kinase diseases associated, comprise the compound that the patient is given at least a claim 1 of significant quantity.
43. the method for claim 42, wherein said aurora kinase are aurora-A.
44. the method for claim 42, wherein said aurora kinase are aurora-B.
45. the method for claim 42, wherein said aurora kinase are aurora-C.
46. treat the patient and the method Tyrosylprotein kinase diseases associated, comprise the compound that the patient is given at least a claim 1 of significant quantity.
47. the method for claim 46, wherein said Tyrosylprotein kinase is selected from VEGF-R2, EGFR, HER2, SRC, JAK and TEK.
48. the method for claim 47, wherein said Tyrosylprotein kinase are VEGF-R2.
49. the method for claim 47, wherein said Tyrosylprotein kinase are EGFR.
50. the suppression therapy patient and method Pim-1 kinases diseases associated comprise the compound that the patient is given at least a claim 1 of significant quantity.
51. treat the patient and method c-Met kinases diseases associated, comprise the compound that the patient is given at least a claim 1 of significant quantity.
52. the method for claim 51, wherein said c-Met kinases is c-Met.
53. treat the patient and method MEK kinases diseases associated, comprise the compound that the patient is given at least a claim 1 of significant quantity.
54. the method for claim 53, wherein said mek kinases is MEK-1.
55. treatment patient's method for cancer comprises the compound that the patient is given at least a claim 1 of significant quantity.
56. the method for claim 55 comprises at least a other carcinostatic agent that the patient is given significant quantity in addition, wherein said other carcinostatic agent is different from the compound of claim 1.
57. the method for claim 55, wherein said cancer are bladder cancer, mammary cancer, colorectal carcinoma, kidney, liver cancer, other cancer of the cancer of the brain or central nervous system, small cell lung cancer, nonsmall-cell lung cancer, head and neck cancer, the esophageal carcinoma, carcinoma of gallbladder, ovarian cancer, carcinoma of the pancreas, cancer of the stomach, neck cancer, thyroid carcinoma, prostate cancer, uterus carcinoma, skin carcinoma, leukemia, non-Hodgkin lymphoma, Hodgkin lymphoma, non-Hodgkin lymphoma, fibrosarcoma, rhabdosarcoma, myelomatosis, spermocytoma, malignant teratoma, osteosarcoma, the pigment xeroderma, angling spinose cell's knurl, Tiroidina trichocarcinoma or Kaposi sarcoma.
58. the method for claim 56, wherein said at least a other carcinostatic agent is selected from cytostatic agent, cis-platinum, A Luo platinum, Dx, taxotere, taxol, Etoposide, irinotecan, Kan Sida, topotecan, taxol, docetaxel, esperamicin, tamoxifen, 5-fluor-uracil, methotrexate, Temozolomide, endoxan, SCH 66336, R115777, L778123, BMS 214662, Iressa, Te Luokai, the antibody of EGFR, imatinib mesylate, because of wound-A, Interferon, rabbit, interleukin-, ara-C, gemcitabine, Uramustine, mustargen, ifosfamide, melphalan, Chlorambucil, pipobroman, Tretamine, triethylene sulfo-phosphamidon, busulfan, carmustine, lomustine, streptozocin, Dacarbazine, floxuridine, cytosine arabinoside, Ismipur, 6-thioguanine, fludarabine phosphate, pentostatin, vinealeucoblastine(VLB), vincristine(VCR), vindesine, vinorelbine, bleomycin, dactinomycin, daunorubicin, epirubicin, idarubicin, Plicamycin, deoxycoformycin, Mitomycin-C, the L-Asparaginase, teniposide, the female alcohol of 17 alpha-acetylenes, stilboestrol, testosterone, prednisone, FL, dromostanolone propionate, Testolactone, the acetate megestrol, methylprednisolone, Synrotabs, Prednisolone Acetate, triamcinolone, Chlortrianisoestrol, hydroxyprogesterone, aminoglutethimide, estramustine, Amen, Leuprolide, flutamide, toremifene, goserelin, carboplatin, hydroxyurea, amsacrine, Procarbazine, mitotane, mitoxantrone, LEVAMISOLE HCL, nvelbine, Anastrozole, letrozole, gemcitabine, capecitabine, Lei Luoshafen, Du Luoshafen, Hexamethylmelamine, A Wasiting, Trastuzumab, hectogram sand, ten thousand jade-like stones, Ze Waling, white arsenic, xeloda, the porphines nurse, Erbitux, liposome, plug is for group, altretamine, melphalan, Herceptin, letrozole, fulvestrant, Exemestane, sharp appropriate Xidan is anti-, C225, how western, Ang Take, Supreme Being's baud, Mai Luotage, the bank Paasche, comfortable, A Fadabei Bo Ting, Xin Sita, Kevin Si, SU11248, and PTK787.
59. the method for claim 55 comprises giving radiotherapy to the patient in addition.
60. the method for claim 56 comprises giving radiotherapy to the patient in addition.
CN2008801234697A 2007-10-29 2008-10-27 Diamido thiazole derivatives as protein kinase inhibitors Pending CN101910164A (en)

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