CN101906407A - Complex enzyme and application thereof - Google Patents
Complex enzyme and application thereof Download PDFInfo
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- CN101906407A CN101906407A CN2010102217310A CN201010221731A CN101906407A CN 101906407 A CN101906407 A CN 101906407A CN 2010102217310 A CN2010102217310 A CN 2010102217310A CN 201010221731 A CN201010221731 A CN 201010221731A CN 101906407 A CN101906407 A CN 101906407A
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- hemicellulase
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Abstract
The invention discloses complex enzyme, consisting of the following components by weight percent: 35-50% of hemicellulase, 10-15% of glutaminetransaminage and the balance of phytase. The invention further discloses the application method of the complex enzyme. The invention is applied to the production of wheat gluten, can improve the yield of the wheat gluten while reducing the energy consumption, further can reduce the production period, is easy to clean, can save water and power consumption and reduce cost.
Description
Technical field
The present invention relates to a kind of prozyme, is a kind of being used to produce the prozyme that gluten powder can improve the gluten powder yield and cut down the consumption of energy, and especially also relates to the application of this prozyme, belongs to the food fermentation technical field.
Background technology
Gluten powder is a kind of good flour-dough improver, is widely used in the production of bread, noodles, instant noodles, also can be used in the meat product as water-holding agent.At present domestic also gluten powder as a kind of gluten fortifier of green flour efficiently, use it for the production of high-toughness flour, tailored flour for bread, addition is unrestricted.Gluten powder still increases the effective ways of plant protein content in the food.Along with the requirement of Economic development and domestic food industrial development, in further expansion, demand also has further increase to the range of application of gluten powder.
There is big gap in gluten powder manufacturing enterprise aspect yield both at home and abroad, and domestic most of enterprises can isolate the gluten powder of better quality and larger amt in the wheat starch production process, and recovery rate is higher.Produce 200000 tons wheat starch production enterprise per year as France one family, produce gluten powder output up to more than 50,000 tons/year, its isolating gluten powder yield reaches more than 90%.And the aleuronat content of China is between 11-15%, and its isolating gluten powder yield only reaches 80%.This illustrates that we also have bigger room for promotion at the gluten powder yield.Zymin has extraordinary effect in raising gluten powder yield with aspect cutting down the consumption of energy.
Summary of the invention
At the deficiency that prior art exists, one of technical problem to be solved by this invention provides a kind of prozyme that can improve the gluten powder yield and cut down the consumption of energy; Two of technical problem to be solved provides the application method of described prozyme.
For solving the problems of the technologies described above, one of technical scheme that the present invention takes is: a kind of prozyme, and its one-tenth by following weight per-cent is grouped into: hemicellulase, 35-50%; Glutamine transaminage, 10-15%; Phytase, surplus.
Above-mentioned hemicellulase is made of following component in percentage by weight: beta-glucanase: 75%; Zytase, 25%.
Above-mentioned Transglutaminase EC2.3.2.13 is a trans-glutaminases.
Above-mentioned phytase is a plant hydrochlorate enzyme.
The application method of above-mentioned prozyme comprises following content: the prozyme of dry-matter raw material 0.1-0.2 ‰ mass ratio is added in the process water dissolve, add the dry-matter raw material then and carry out with face, wash one's face in process water, obtain gluten powder after pulverizing oven dry.
Prozyme of the present invention, beta-glucan and the xylan of its a large amount of hemicellulose enzyme component in can hydrolysis material destroys its long-chain and reticulated structure, discharges by the technology active substances such as protein of its complexing, wash out rate thereby improve gluten, improve raw material availability; In addition, hemicellulase has been removed viscous substances such as beta-glucan and xylan, helps the dough washing, can reduce power consumption, water saving.Glutamine transaminage composition in this prozyme, be a kind of can catalysis the epsilon-amino of Methionin cause the enzyme of polymerization of protein with γ-hydroxyl amide group formation covalent linkage of L-glutamic acid.Because the protein after the polymerization often shows than good functional property more before the polymerization, it is agglomerating that gluten is easy to, and improves the gluten yield.Phytase composition in this prozyme can destroy the chelate structure of phytate, discharges the protein that is chelated, and improves yield; After phytase decomposed the phytic acid macromole and is small molecules, dough viscosity reduced, can minimizing and face, the power consumption of washing one's face.
The present invention can improve the gluten powder yield and cut down the consumption of energy, and can shorten the production cycle, and is easy to washing, conserves water, and reduces dough viscosity, saves power consumption, reduces cost.
Embodiment
The present invention is described in more detail below in conjunction with embodiment.
The prozyme technological process of production of the present invention is: the mensuration enzyme was lived after each dhdps enzyme entered factory, and whether bacterial detection exceeds standard.Qualified after testing dhdps enzyme is transported to by following weight ratio according to enzyme activity and is respectively specified dog-house to enter the GMP workshop: hemicellulase, 35-50%; Glutamine transaminage, 10-15%; Phytase, 35-55%; Packed by setting specification by automatic packing machine behind each dhdps enzyme mixing, stochastic sampling detects qualified back packaging and warehousing again.
Embodiment 1
A kind of prozyme, by aforementioned production technique preparation, its one-tenth by following weight per-cent is grouped into: hemicellulase, 35%; Trans-glutaminases, 10%; Plant hydrochlorate enzyme, 55%.Hemicellulase is made up of beta-glucanase and zytase, and wherein beta-glucanase accounts for 75%, zytase accounts for 25% weight part.
The application method of this prozyme is: the prozyme of dry-matter raw material 0.2 ‰ mass ratioes is added in the process water dissolve, add the dry-matter raw material then and carry out with face, wash one's face in process water, obtain gluten powder after pulverizing oven dry.
Embodiment 2
A kind of prozyme, by aforementioned production technique preparation, its one-tenth by following weight per-cent is grouped into: hemicellulase, 50%; Trans-glutaminases, 15%; Plant hydrochlorate enzyme, 35%.Hemicellulase is made up of beta-glucanase and zytase, and wherein beta-glucanase accounts for 75%, zytase accounts for 25% weight part.
The application method of this prozyme is: the prozyme of dry-matter raw material 0.1 ‰ mass ratioes is added in the process water dissolve, add the dry-matter raw material then and carry out with face, wash one's face in process water, obtain gluten powder after pulverizing oven dry.
Embodiment 3
A kind of prozyme, by aforementioned production technique preparation, its one-tenth by following weight per-cent is grouped into: hemicellulase, 40%; Trans-glutaminases, 13%; Plant hydrochlorate enzyme, 47%.Hemicellulase is made up of beta-glucanase and zytase, and wherein beta-glucanase accounts for 75%, zytase accounts for 25% weight part.
The application method of this prozyme is: the prozyme of dry-matter raw material 0.15 ‰ mass ratio is added in the process water dissolve, add the dry-matter raw material then and carry out with face, wash one's face in process water, obtain gluten powder after pulverizing oven dry.
Embodiment 4
The prozyme of embodiment 1,2,3 is respectively applied for the production gluten powder as experimental group 1,2,3 according to its application method, and with do not add that prozyme directly carries out with face, the ordinary method of washing one's face, pulverize oven dry preparation gluten powder is organized in contrast and compared experiment, gained the results are shown in Table 1.
By the data in the table 1 as can be seen, application the present invention can improve the gluten powder yield and be up to 1.28 percentage points, can shorten the production cycle, saved time 10 minutes for every batch, and be easy to washing, every batch can be reduced washing times one time, conserves water 0.4 cubic metre.And the reduction dough viscosity is saved power consumption.
The contrast and experiment of table 1 control group and each experimental group
Claims (5)
1. prozyme, its one-tenth by following weight per-cent is grouped into: hemicellulase, 35-50%; Glutamine transaminage, 10-15%; Phytase, surplus.
2. prozyme according to claim 1 is characterized in that: described hemicellulase is made of following component in percentage by weight: beta-glucanase: 75%; Zytase, 25%.
3. prozyme according to claim 2 is characterized in that: described Transglutaminase EC2.3.2.13 is a trans-glutaminases.
4. prozyme according to claim 3 is characterized in that: described phytase is a plant hydrochlorate enzyme.
5. the application method of each described prozyme of claim 1 to 4, it comprises following content: the prozyme of dry-matter raw material 0.1-0.2 ‰ mass ratio is added in the process water dissolve, in process water, add the dry-matter raw material then and carry out with face, wash one's face, pulverize oven dry back preparation gluten powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102217310A CN101906407A (en) | 2010-07-09 | 2010-07-09 | Complex enzyme and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102217310A CN101906407A (en) | 2010-07-09 | 2010-07-09 | Complex enzyme and application thereof |
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| CN101906407A true CN101906407A (en) | 2010-12-08 |
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| CN2010102217310A Pending CN101906407A (en) | 2010-07-09 | 2010-07-09 | Complex enzyme and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102776165A (en) * | 2011-05-09 | 2012-11-14 | 白银赛诺生物科技有限公司 | Starch wheat compound enzyme and method of applying same in preparation of starch wheat and vital gluten |
| CN102907557A (en) * | 2012-10-24 | 2013-02-06 | 安徽安特食品股份有限公司 | Production technique of modified vital gluten |
| CN107118281A (en) * | 2017-05-16 | 2017-09-01 | 润禾粉业南通有限公司 | A kind of wheaten starch preparation method |
| CN107927316A (en) * | 2017-11-16 | 2018-04-20 | 宁夏夏盛实业集团有限公司 | A kind of application of complex enzyme during three phase centrifugation method produces Gluten |
| CN108783207A (en) * | 2018-05-28 | 2018-11-13 | 浚县中鹤营养面业有限公司 | A kind of high-content buckwheat vermicelli and preparation method thereof |
| CN111518781A (en) * | 2019-07-31 | 2020-08-11 | 江南大学 | Glutamine transaminase complex enzyme and application thereof in artificial meat processing |
| CN113907255A (en) * | 2021-10-25 | 2022-01-11 | 广东以琳食品有限公司 | Medium-temperature enzymolysis process and device for preparing flour special for shredded cake |
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| CN1055861A (en) * | 1990-04-27 | 1991-11-06 | 张玉成 | The technology of the air-dry system vital wheat gluten of low temperature |
| CN1640270A (en) * | 2004-01-14 | 2005-07-20 | 史云峰 | Clean production process of gluten powder |
| CN1961714A (en) * | 2006-11-17 | 2007-05-16 | 江苏天福莱饲料发展有限公司 | A feedstuff for marine lives and pre-maturation process |
| CN101168764A (en) * | 2007-11-05 | 2008-04-30 | 中华全国供销合作总社南京野生植物综合利用研究院 | Method for producing anti-oxidation active peptide by glutelin powder biological enzyme method |
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Patent Citations (4)
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| CN1055861A (en) * | 1990-04-27 | 1991-11-06 | 张玉成 | The technology of the air-dry system vital wheat gluten of low temperature |
| CN1640270A (en) * | 2004-01-14 | 2005-07-20 | 史云峰 | Clean production process of gluten powder |
| CN1961714A (en) * | 2006-11-17 | 2007-05-16 | 江苏天福莱饲料发展有限公司 | A feedstuff for marine lives and pre-maturation process |
| CN101168764A (en) * | 2007-11-05 | 2008-04-30 | 中华全国供销合作总社南京野生植物综合利用研究院 | Method for producing anti-oxidation active peptide by glutelin powder biological enzyme method |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102776165A (en) * | 2011-05-09 | 2012-11-14 | 白银赛诺生物科技有限公司 | Starch wheat compound enzyme and method of applying same in preparation of starch wheat and vital gluten |
| CN102907557A (en) * | 2012-10-24 | 2013-02-06 | 安徽安特食品股份有限公司 | Production technique of modified vital gluten |
| CN102907557B (en) * | 2012-10-24 | 2014-03-26 | 安徽安特食品股份有限公司 | Production technique of modified vital gluten |
| CN107118281A (en) * | 2017-05-16 | 2017-09-01 | 润禾粉业南通有限公司 | A kind of wheaten starch preparation method |
| CN107927316A (en) * | 2017-11-16 | 2018-04-20 | 宁夏夏盛实业集团有限公司 | A kind of application of complex enzyme during three phase centrifugation method produces Gluten |
| CN108783207A (en) * | 2018-05-28 | 2018-11-13 | 浚县中鹤营养面业有限公司 | A kind of high-content buckwheat vermicelli and preparation method thereof |
| WO2019228058A1 (en) * | 2018-05-28 | 2019-12-05 | 河南中鹤研究院有限公司 | High-content buckwheat noodles and preparation method therefor |
| US20200236980A1 (en) * | 2018-05-28 | 2020-07-30 | Jiangnan University | High-content Buckwheat Dried Noodles and Processing Method Thereof |
| CN108783207B (en) * | 2018-05-28 | 2022-02-11 | 河南中鹤研究院有限公司 | High-content buckwheat fine dried noodles and making method thereof |
| CN111518781A (en) * | 2019-07-31 | 2020-08-11 | 江南大学 | Glutamine transaminase complex enzyme and application thereof in artificial meat processing |
| CN113907255A (en) * | 2021-10-25 | 2022-01-11 | 广东以琳食品有限公司 | Medium-temperature enzymolysis process and device for preparing flour special for shredded cake |
| CN113907255B (en) * | 2021-10-25 | 2024-04-19 | 广东以琳食品有限公司 | Medium-temperature enzymolysis process and device for preparing flour special for hand-held cakes |
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Address after: Miao road Laoshan District 266061 Shandong city of Qingdao province Shandong No. 29 high building 12A07 Applicant after: Qingdao Weilan Biology Group Co., Ltd. Address before: Room 12A07, No. 29 building, 266061 Shandong Shandong high-speed Miao road of city of Qingdao Province Applicant before: Qingdao Continent Biotech Co., Ltd. |
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Application publication date: 20101208 |