CN101906386A - Method for hydrolyzing streptococcus lactis and method for producing natamycin using hydrolyzed liquid of streptococcus lactis - Google Patents
Method for hydrolyzing streptococcus lactis and method for producing natamycin using hydrolyzed liquid of streptococcus lactis Download PDFInfo
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Abstract
The invention provides a method for hydrolyzing streptococcus lactis and a method for producing natamycin using hydrolyzed liquid of streptococcus lactis. The invention relates to a method for hydrolyzing thalluses and a method for producing the natamycin by using hydrolyzed liquid. The invention solves the problems that production cost is high and industrial pollution is caused by the direct discharging of the thalluses obtained by extracting nisin in the existing method for producing the natamycin. The invention adopts animal protease or hydrochloric acid to promote the hydrolysis of the streptococcus lactis. The method for producing the natamycin comprises the following steps: adding yeast extract powder, glucose, hydrolyzed liquid of streptococcus lactis and defoamer to a fermentation tank and performing fermentation for 80h to obtain the natamycin. The invention uses the waste, namely, streptococcus lactis thalluses obtained by the separation in the extracting process of producing the nisin to obtain polypeptide and amino acid by hydrolytic treatment; and the polypeptide and the amino acid are used for replacing soybean to separate protein to produce the natamycin, thus recycling waste materials. The invention not only reduces the emission of pollutants, but also lowers the cost for producing the natamycin.
Description
Technical field
The present invention relates to hydrolysis thalline method and use the method that hydrolyzed solution is produced tennecetin.
Background technology
Tennecetin is that a kind of polyene hydrocarbon macrolide of streptomycete fermentation generation is antifungal agents based, its molecule is a kind of active ring-type tetraene compound that has, contain the crystal water more than 3, be filamentous fungus inhibitor such as the yeast of a kind of natural, wide spectrum, highly effective and safe and mould, it not only can suppress fungi, can also prevent the generation of mycotoxins.Tennecetin is present unique in the world antimycotic microbiological antiseptic.But because the tennecetin fermentation yield is low, the rate of recovery is low, has obviously strengthened and has produced the cost of tennecetin, thereby found cheap fermentation substrate particularly important.
In extracting the nisin process, separate the thalline that obtains, just sell as tropina, it is low to make a profit, if with its direct discharging, also can cause industrial pollution.
Summary of the invention
The objective of the invention is in order to solve the existing method production cost height of producing tennecetin, the extraction nisin separates the thalline that obtains and directly discharges, can cause the problem of industrial pollution, hydrolysis thalline method is provided and use the method that hydrolyzed solution is produced tennecetin.
Hydrolysed lactic acid suis method of the present invention is as follows: the pH value of streptococcus acidi lactici is transferred to 7.5, press 0.45% of streptococcus acidi lactici thalline weight in wet base and add animal protease, hydrolysis 8 hours under 55 ℃ condition then is warmed up to 85 ℃ again, kept 10 minutes, and promptly got streptococcus lactis hydrolyzate.
The method of producing tennecetin with streptococcus lactis hydrolyzate is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of method for preparing and defoamer join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.48～7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min～400 r/min, tank pressure is 0.04MPa～0.06MPa, the fermentor tank rotating speed is 600r/min, react under the condition of dissolved oxygen＞50% that the pH of mixed value is 5.9～6.1 to fermentation shake flask or the fermentor tank, adopting mass concentration then is that the pH of mixed value is 5.9～6.1 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets tennecetin; To soak the concentration of powder be 6g/L to yeast in fermentation shake flask or the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L～370.8g/L, and the concentration of defoamer is 0.1g/L～0.2g/L.
Second kind of hydrolysed lactic acid suis of the present invention method is as follows: adopt hydrochloric acid that the pH value of streptococcus acidi lactici is transferred to 1.0, hydrolysis 12 hours under 121 ℃ condition then promptly gets streptococcus lactis hydrolyzate.
The method of producing tennecetin with streptococcus lactis hydrolyzate is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that second kind of hydrolysed lactic acid suis hydrolyzed solution and defoamer join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.48～7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min～400 r/min, tank pressure is 0.04MPa～0.06MPa, the rotating speed of fermentor tank is 600r/min, react under dissolving＞50% the condition that the pH of mixed value is 5.9～6.1 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 5.9～6.1 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets tennecetin; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L～379.88g/L, and the concentration of defoamer is 0.1 g/L～0.2g/L.
Above-mentioned defoamer is an XP-010 General Foods Corporation defoamer.
The present invention utilizes to produce and separates the waste material-streptococcus acidi lactici thalline that obtains in the nisin leaching process, obtain polypeptide and amino acid through hydrolysis treatment, substitute soybean protein isolate as nitrogenous source and produce tennecetin, thereby turn waste into wealth, not only reduce the discharging of pollutent, also reduced the cost of producing tennecetin.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: hydrolysed lactic acid suis method is as follows in the present embodiment: the pH value of streptococcus acidi lactici is transferred to 7.5, press 0.45% of streptococcus acidi lactici thalline weight in wet base and add animal protease, hydrolysis 8 hours under 55 ℃ condition then, be warmed up to 85 ℃ again, kept 10 minutes, and promptly got streptococcus lactis hydrolyzate.
The streptococcus lactis hydrolyzate total nitrogen content of present embodiment preparation is 0.922% after testing.
The manufacturer of used animal proteolytic enzyme is Pangbo Bioengineering Co Ltd, Nanning in the present embodiment.
Embodiment two: hydrolysed lactic acid suis method is as follows in the present embodiment: adopt hydrochloric acid that the pH value of streptococcus acidi lactici is transferred to 1.0, hydrolysis 12 hours under 121 ℃ condition then promptly gets streptococcus lactis hydrolyzate.
The streptococcus lactis hydrolyzate total nitrogen content of present embodiment preparation is 0.9% after testing.
The manufacturer of used animal proteolytic enzyme is Pangbo Bioengineering Co Ltd, Nanning in the present embodiment.
Embodiment three: the method with streptococcus lactis hydrolyzate production tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of embodiment one preparation and defoamer join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.48～7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min～400 r/min, tank pressure is 0.04MPa～0.06MPa, the fermentor tank rotating speed is 600r/min, react under the condition of dissolved oxygen＞50% that the pH of mixed value is 5.9～6.1 to fermentation shake flask or the fermentor tank, adopting mass concentration then is that the pH of mixed value is 5.9～6.1 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets tennecetin; To soak the concentration of powder be 6g/L to yeast in fermentation shake flask or the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L～370.8g/L, and the concentration of defoamer is 0.1g/L～0.2g/L.
Embodiment four: what present embodiment and embodiment three were different is that described defoamer is an XP-010 General Foods Corporation defoamer.Other is identical with embodiment three.
Embodiment five: what present embodiment and embodiment three were different is that described initial pH value is 7.5.Other is identical with embodiment three.
Embodiment six: what present embodiment and embodiment three were different is that described fermentor tank tank pressure is 0.05MPa.Other is identical with embodiment three.
Embodiment seven: present embodiment and embodiment three are different is that the liquid amount of fermentor tank is 7.5L.Other is identical with embodiment three.
Embodiment eight: what present embodiment and embodiment three were different is that shaking speed is 280r/min～380 r/min.Other is identical with embodiment three.
Embodiment nine: what present embodiment and embodiment three were different is that shaking speed is 300 r/min.Other is identical with embodiment three.
Embodiment ten: the method with streptococcus lactis hydrolyzate production tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of embodiment two preparations and defoamer join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.48～7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min～400 r/min, tank pressure is 0.04MPa～0.06MPa, the rotating speed of fermentor tank is 600r/min, react under dissolving＞50% the condition that the pH of mixed value is 5.9～6.1 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 5.9～6.1 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets tennecetin; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L～379.88g/L, and the concentration of defoamer is 0.1 g/L～0.2g/L.
Embodiment 11: what present embodiment and embodiment ten were different is that described defoamer is an XP-010 General Foods Corporation defoamer.Other is identical with embodiment ten.
Embodiment 12: what present embodiment and embodiment ten were different is that described initial pH value is 7.5.Other is identical with embodiment ten.
Embodiment 13: what present embodiment and embodiment ten were different is that described fermentor tank tank pressure is 0.05MPa.Other is identical with embodiment ten.
Embodiment 14: present embodiment and embodiment ten are different is that the liquid amount of fermentor tank is 7.5L.Other is identical with embodiment ten.
Embodiment 15: what present embodiment and embodiment ten were different is that shaking speed is 280r/min～380 r/min.Other is identical with embodiment ten.
Embodiment 16: what present embodiment and embodiment ten were different is that shaking speed is 300 r/min.Other is identical with embodiment ten.
Embodiment 17: the method for producing tennecetin in the present embodiment is as follows: yeast is soaked powder, it is in the fermentor tank of 6L that glucose and soybean protein isolate join fermentation shake flask or the liquid amount that liquid amount is 30ml, in initial pH value is 7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min, the fermentor tank rotating speed is 600r/min, tank pressure is 0.04MPa, react under the condition of dissolved oxygen＞50% that the pH of mixed value is 6 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 6 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets the fermented liquid that tennecetin content is 11.2g/L; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, soybean protein isolate is 26g/L.
Used soybean protein isolate total nitrogen content is 12.21% in the present embodiment.
Embodiment 18: the method for producing tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, the streptococcus lactis hydrolyzate of embodiment one preparation and soybean protein isolate joins in the fermentation shake flask that liquid amount is 30mL or liquid amount is in the fermentor tank of 6L, in initial pH value is 7.49, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min, tank pressure is 0.04MPa, the fermentor tank rotating speed is 600r/min, react under the condition of dissolved oxygen (DO)＞50% that the pH of mixed value is 6 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 6 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets the fermented liquid that tennecetin content is 10.72g/L; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is that the concentration of 86.08g/L, soybean protein isolate is 19.5g/L.
Use tennecetin content in the UV spectrophotometer measuring fermented liquid in the present embodiment.
Embodiment 19: the method for producing tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of embodiment one preparation and soybean protein isolate join fermentation shake flask or the liquid amount that liquid amount is 30ml, in initial pH value is 7.5, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min, the fermentor tank rotating speed is 600r/min, tank pressure is 0.04MPa, react under the condition of dissolved oxygen (DO)＞50% that the pH of mixed value is 6 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 6 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets the fermented liquid that tennecetin content is 10.18g/L; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is that the concentration of 172.16g/L, soybean protein isolate is 13g/L.
Use tennecetin content in the UV spectrophotometer measuring fermented liquid in the present embodiment.
Embodiment 20: the method for producing tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of embodiment one preparation and soybean protein isolate join fermentation shake flask or the liquid amount that liquid amount is 30ml, in initial pH value is 7.49, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min, the fermentor tank rotating speed is 600r/min, the fermentor tank tank pressure is 0.04MPa, react under the condition of dissolved oxygen (DO)＞50% that the pH of mixed value is 6 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 6 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets the fermented liquid that tennecetin content is 10.10g/L; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is that the concentration of 258.24g/L, soybean protein isolate is 6.5g/L.
Use tennecetin content in the UV spectrophotometer measuring fermented liquid in the present embodiment.
Embodiment 21: the method with streptococcus lactis hydrolyzate production tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of embodiment one preparation and XP-010 General Foods Corporation defoamer join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.48, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min, the fermentor tank rotating speed is 600r/min, the fermentor tank tank pressure is 0.04MPa, react under the condition of dissolved oxygen (DO)＞50% that the pH of mixed value is 6 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 6 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly get tennecetin, tennecetin content is 9.66g/L; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L, and the concentration of XP-010 General Foods Corporation defoamer is 0.1～0.2g/L.
By embodiment 17 to the contrast of present embodiment as can be seen streptococcus lactis hydrolyzate can substitute soybean protein isolate fully and be used to prepare tennecetin as nitrogenous source.
Embodiment 22: the method for producing tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of embodiment two preparations and soybean protein isolate join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min, the fermentor tank rotating speed is 600r/min, the fermentor tank tank pressure is 0.04MPa, react under the condition of dissolved oxygen (DO)＞50% that the pH of mixed value is 6 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 6 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets the fermented liquid that tennecetin content is 10.65g/L; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is that the concentration of 172.16g/L, soybean protein isolate is 13g/L.
Use tennecetin content in the UV spectrophotometer measuring fermented liquid in the present embodiment.
Embodiment 23: the method with streptococcus lactis hydrolyzate production tennecetin in the present embodiment is as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of embodiment two preparations and XP-010 General Foods Corporation defoamer join fermentation shake flask or the liquid amount that liquid amount is 30ml, in initial pH value is 7.50, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min, the fermentor tank tank pressure is 0.04MPa, the rotating speed of fermentor tank is 600r/min, react under the condition of dissolved oxygen＞50% that the pH of mixed value is 6 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 6 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly get tennecetin, tennecetin content is 10.96g/L; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L, and the concentration of XP-010 General Foods Corporation defoamer is 0.1～0.2g/L.
By the contrast of embodiment 17, embodiment 22 and present embodiment as can be seen streptococcus lactis hydrolyzate can substitute soybean protein isolate fully and be used to prepare tennecetin as nitrogenous source.
Claims (10)
1. hydrolysed lactic acid suis method, it is characterized in that hydrolysed lactic acid suis method is as follows: the pH value of streptococcus acidi lactici is transferred to 7.5, press 0.45% of streptococcus acidi lactici thalline weight in wet base and add animal protease, hydrolysis 8 hours under 55 ℃ condition then, be warmed up to 85 ℃ again, kept 10 minutes, and promptly got streptococcus lactis hydrolyzate.
2. hydrolysed lactic acid suis method is characterized in that hydrolysed lactic acid suis method is as follows: adopt hydrochloric acid that the pH value of streptococcus acidi lactici is transferred to 1.0, hydrolysis 12 hours under 121 ℃ condition then promptly gets streptococcus lactis hydrolyzate.
3. produce the method for tennecetin with streptococcus lactis hydrolyzate, it is characterized in that with the method for streptococcus lactis hydrolyzate production tennecetin as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of claim 1 preparation and defoamer join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.48～7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min～400 r/min, tank pressure is 0.04MPa～0.06MPa, the fermentor tank rotating speed is 600r/min, react under the condition of dissolved oxygen＞50% that the pH of mixed value is 5.9～6.1 to fermentation shake flask or the fermentor tank, adopting mass concentration then is that the pH of mixed value is 5.9～6.1 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets tennecetin; To soak the concentration of powder be 6g/L to yeast in fermentation shake flask or the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L～370.8g/L, and the concentration of defoamer is 0.1g/L～0.2g/L.
4. method of producing tennecetin with streptococcus lactis hydrolyzate according to claim 3 is characterized in that described defoamer is an XP-010 General Foods Corporation defoamer.
5. method of producing tennecetin with streptococcus lactis hydrolyzate according to claim 3 is characterized in that described initial pH value is 7.5.
6. method of producing tennecetin with streptococcus lactis hydrolyzate according to claim 3 is characterized in that described fermentor tank tank pressure is 0.05MPa.
7. produce the method for tennecetin with streptococcus lactis hydrolyzate, it is characterized in that with the method for streptococcus lactis hydrolyzate production tennecetin as follows: yeast is soaked powder, glucose, it is in the fermentor tank of 6L that the streptococcus lactis hydrolyzate of claim 2 preparation and defoamer join fermentation shake flask or the liquid amount that liquid amount is 30mL, in initial pH value is 7.48～7.51, temperature is 29 ℃, the shaking speed of fermentation shake flask is 260r/min～400 r/min, tank pressure is 0.04MPa～0.06MPa, the rotating speed of fermentor tank is 600r/min, react under the condition of dissolved oxygen＞50% that the pH of mixed value is 5.9～6.1 to the fermentor tank, adopting mass concentration then is that the pH of mixed value is 5.9～6.1 condition bottom fermentation 80h in 20% the potassium hydroxide solution control fermentor tank, promptly gets tennecetin; To soak the concentration of powder be 6g/L to yeast in the fermentor tank, and the concentration of glucose is that the concentration of 40g/L, streptococcus lactis hydrolyzate is 344.32g/L～379.88g/L, and the concentration of defoamer is 0.1 g/L～0.2g/L.
8. method of producing tennecetin with streptococcus lactis hydrolyzate according to claim 7 is characterized in that described defoamer is an XP-010 General Foods Corporation defoamer.
9. method of producing tennecetin with streptococcus lactis hydrolyzate according to claim 7 is characterized in that described initial pH value is 7.5.
10. method of producing tennecetin with streptococcus lactis hydrolyzate according to claim 7 is characterized in that described fermentor tank tank pressure is 0.05MPa.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827903A (en) * | 2012-08-03 | 2012-12-19 | 武汉中科光谷绿色生物技术有限公司 | Method for increasing yield of natamycin by using Alpinemortierella fermentation waste liquor |
| CN105838761A (en) * | 2016-06-12 | 2016-08-10 | 安泰生物工程股份有限公司 | Method for improving fermentation yield of natamycin |
| CN107047920A (en) * | 2017-03-31 | 2017-08-18 | 上海优久生物科技有限公司 | A kind of preparation method and applications of the lactic acid mycoprotein raw material of high small peptide content |
| CN114130790A (en) * | 2021-11-17 | 2022-03-04 | 河南龙昌机械制造有限公司 | Alkaline hydrolysis system and working method thereof |
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2010
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827903A (en) * | 2012-08-03 | 2012-12-19 | 武汉中科光谷绿色生物技术有限公司 | Method for increasing yield of natamycin by using Alpinemortierella fermentation waste liquor |
| CN102827903B (en) * | 2012-08-03 | 2013-12-18 | 武汉中科光谷绿色生物技术有限公司 | Method for increasing yield of natamycin by using Alpinemortierella fermentation waste liquor |
| CN105838761A (en) * | 2016-06-12 | 2016-08-10 | 安泰生物工程股份有限公司 | Method for improving fermentation yield of natamycin |
| CN105838761B (en) * | 2016-06-12 | 2019-11-29 | 安泰生物工程股份有限公司 | A method of improving natamycin fermentation yield |
| CN107047920A (en) * | 2017-03-31 | 2017-08-18 | 上海优久生物科技有限公司 | A kind of preparation method and applications of the lactic acid mycoprotein raw material of high small peptide content |
| CN114130790A (en) * | 2021-11-17 | 2022-03-04 | 河南龙昌机械制造有限公司 | Alkaline hydrolysis system and working method thereof |
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| CN101906386B (en) | 2012-12-26 |
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