CN101906169A - Process combination for preparing bletilla polysaccharide - Google Patents
Process combination for preparing bletilla polysaccharide Download PDFInfo
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- CN101906169A CN101906169A CN2009103029373A CN200910302937A CN101906169A CN 101906169 A CN101906169 A CN 101906169A CN 2009103029373 A CN2009103029373 A CN 2009103029373A CN 200910302937 A CN200910302937 A CN 200910302937A CN 101906169 A CN101906169 A CN 101906169A
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- bletilla striata
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Abstract
The invention mainly relates to a low-temperature preparation method for bletilla polysaccharide, in particular to a process combination for preparing high-purity bletilla polysaccharide at a low temperature by adopting a core process of homogenization-based extraction and ceramic membrane micro-filtration concentration and using water as a solvent.
Description
Technical field
The present invention relates generally to a kind of low temperature preparation method of BSP, especially a kind of process combination for preparing BSP in conjunction with ceramic membrane micro-filtration simmer down to core process low temperature of extracting based on homogenization.
Background technology
The bletilla striata (Bletilla striata (Thunb.) Reichb.f) is a orchid family bletilla striata platymiscium, claims the little bletilla striata, lotus and grass, snow such as end etc. again, uses as traditional Chinese medicine over the past thousands of years always, and its major function is to hold back sore hemostasis, tonifying lung, detumescence and promoting granulation etc.Contain a large amount of water-soluble fluidity polysaccharide in the bletilla striata bulb, its chemical ingredients is a glucomannan, is the main function composition of the bletilla striata.
Purity of polysaccharide is greater than 90% bletilla striata glue, can dissolve to form heavy-gravity wetting ability glue in water, and 2% bletilla striata glue solution viscosity is 9～12mm
2/ s is a kind of good whole food thickening material.Bletilla striata glue pharmaceutically has the effect anti-oxidant, that external application promotes the keratinocyte migration of taking orally, can be used as arterial embolic agent and be used for liver cancer treatment, also can be used for treating hemostasis in ulcerative colitis, the operation, upper gastrointestinal massive hemorrhage and promotion wound skin healing etc.; In foodstuffs industry, can be used as the natural fruit coating antistaling agent important source material, can improve system stability such as oil-in-water type essence, water-latex type local flavor flavor oil, milk-contained drink; Bletilla striata glue still is natural daily use chemicals additive of a kind of ideal and functional ingredient.Bletilla striata glue makeup split, stimulate circulation, improve the skin-nourishing situation, prevent that the wrinkle generation from having obvious effect control skin pernio and glass, and do not have toxic side effects.
Extract BSP usually with water or the hot extracting mode of aqueous ethanol, because the viscosity and the water-soluble bloated characteristic of BSP, in leaching process, must replenish new extraction solvent in good time or increase and extract batch, production cycle is long, quantity of solvent is big, energy consumption is higher, the purity of BSP generally is lower than 80% in the product of preparation, and needs employing ethanol repeatedly measures such as precipitation, ether and acetone class organic solvent washing all improves with its purity.
Summary of the invention
The low temperature preparation method that the purpose of this invention is to provide a kind of BSP, especially a kind of process combination for preparing BSP in conjunction with the low temperature of ceramic membrane micro-filtration simmer down to core process of extracting based on homogenization.
The present invention realizes by adopting following technical proposals: bletilla striata raw material through clean, pulverizing, homogenization, centrifugal, filtering, micro-filtration concentrate, make gelationus liquid BSP, or further concentrated solution alcohol precipitation, drying made the solid BSP.
The present invention adopts following concrete steps:
1. clean: the inorganic impurities such as silt that wash most plant material with extract at room temperature with water.
2. pulverize: the bletilla striata is crushed to below the 1.5mm.
3. mix: add the extraction water of the following temperature of normal temperature in the bletilla striata of pulverizing, making total amount of water is 20～300 times of bletilla striata medicinal material powder charging capacity, and mixes into uniform suspension.
4. homogenization: with uniform suspension clarifixator homogenization.
5. sedimentation is centrifugal: through the centrifugal water-insoluble particle of removing of sedimentation.
6. media filtration: centrifugate is through media filtration no visual visible particle to the filtrate.
7. micro-filtration concentrates: the ceramic membrane filter device with filtering accuracy 2000～100nm carries out tangential flow filtration, collects the micro-filtration that needs concentration and keeps liquid.Get the colloidal liquid BSP.
8. alcohol precipitation: in gluey BSP, add ethanol, collecting precipitation.
9. dry: the precipitation vacuum-drying of alcohol precipitation to moisture below 8%, is pulverized, the solid BSP.
Pulverizing of the present invention is meant with the pulverizer of prepackage 2mm following aperture web plate the bletilla striata is ground into powder below the 1.5mm that generally pre-install the web plate of 0.5～1.5mm, the best is the web plate of 0.8～1.2mm.
The extraction water of the following temperature of normal temperature of the present invention is meant the primary reverse osmosis water below 30 ℃, general 15～30 ℃, and best 20～25 ℃.
The consumption of extraction water of the present invention is 20～300 times of bletilla striata medicinal material powder charging capacity, is generally 50～200 times, and the best is 80～120 times.
Homogenization of the present invention is meant with high pressure homogenizer and handles bletilla striata powder and the suspension that extracts water, homogenate after the processing is left standstill do not produce tangible precipitation in 2 hours; The processing pressure of high pressure homogenizer can be selected between 5～75MPa, is generally 20～60MPa, and the best is 45～55MPa.
Media filtration of the present invention is meant that medium filter is to clarification after filtration with sedimentation centrifugate, and filtration medium comprises one or more the mixing in filter cloth, filter paper and flocculating aids talcum powder, gac, the diatomite.General diatomite and the talcous arbitrary ratio mixture of using, the best is selected diatomite and 1: 1 mixture of talcum powder for use.
The mode of media filtration of the present invention can be press filtration, vacuum filtration, and flocculating aids can be contained in advance and overlay filtration on the strainer, also can be to mix slurry to filter with sedimentation centrifugate, or overlay filtration and mix the filtering combination of slurry.
Micro-filtration of the present invention is meant the ceramic membrane filter of filtering accuracy 2000nm～100nm, generally selects the ceramic membrane of 800nm～100nm for use, and the best is selected the ceramic membrane combination of 500nm～200nm for use.
Micro-filtration of the present invention keeps liquid, is meant the trapped fluid of ceramic membrane filter, holds back liquid measure and generally is equivalent to 2～20 times of bletilla striata medicinal material powder charging capacity, is generally 5～15 times, and the best is 6～10 times.
The present invention has the following advantages:
1. cold operation: except that drying process, all the other operations are all carried out at normal temperature, have reduced the problems such as color burn in the technological process, have solved the cold cut problem of product simultaneously.
2. adopt high-pressure homogeneous homogenization mode, improve cell crashing ratio as far as possible, extraction efficiency obviously improves.
3. process serialization: all processes can be finished in dynamic successive processes.
Embodiment
Ultimate principle for a better understanding of the present invention, we will be specifically addressed principle of the present invention by following examples.Following description, can make the present technique field other people under various situation, use the present invention, deacclimatize the different application of expection with various correction.
Any additional application of the explanation invention principle that this paper enumerates all relates to the technology of association area and to the ownership of these open materials, all is considered as included in the scope of claim involved in the present invention.Contents such as the solvent ratio that relates to, filtration medium, micro-filtration precision are to further specific descriptions of the present invention but not limitation of the present invention.
Embodiment 1
The preparation of bletilla striata Crude polysaccharides:
Major equipment: Universalpulverizer, auger tank, high pressure homogenizer, tripod pendulum type batch centrifugal, kieselguhr filter, 500+200nm ceramic membrane filter device, Alcohol-settling tank, vacuum drying oven.
Raw material: bletilla striata medicine materical crude slice 5kg.
Preparation process:
1. pulverizing: the Universalpulverizer with prepackage 1.2mm screen cloth is pulverized bletilla striata medicine materical crude slice, makes all and sieves by No. two, gets bletilla striata meal.
2. mix: bletilla striata meal and 500kg20? primary reverse osmosis water put altogether in the auger tank, stirred 15 minutes, make to be mixed into bletilla striata suspension.
3. homogenization: bletilla striata suspension input high pressure is pressed clarifixator, regulate high pressure homogenizer pump housing pressure 45 embodiments
Ultimate principle for a better understanding of the present invention, we will be specifically addressed principle of the present invention by following examples.Following description, can make the present technique field other people under various situation, use the present invention, deacclimatize the different application of expection with various correction.
Any additional application of the explanation invention principle that this paper enumerates all relates to the technology of association area and to the ownership of these open materials, all is considered as included in the scope of claim involved in the present invention.Contents such as the solvent ratio that relates to, filtration medium, micro-filtration precision are to further specific descriptions of the present invention but not limitation of the present invention.
Embodiment 1
The preparation of bletilla striata Crude polysaccharides:
Major equipment: Universalpulverizer, auger tank, high pressure homogenizer, tripod pendulum type batch centrifugal, kieselguhr filter, 500+200nm ceramic membrane filter device, Alcohol-settling tank, vacuum drying oven.
Raw material: bletilla striata medicine materical crude slice 5kg.
Preparation process:
1. pulverizing: the Universalpulverizer with prepackage 1.2mm screen cloth is pulverized bletilla striata medicine materical crude slice, makes all and sieves by No. two, gets bletilla striata meal.
2. mix: bletilla striata meal and 500kg20? primary reverse osmosis water put altogether in the auger tank, stirred 15 minutes, make to be mixed into bletilla striata suspension.
3. homogenization: bletilla striata suspension input high pressure is pressed clarifixator, regulate high pressure homogenizer pump housing pressure 45～55MPa.
4. centrifugal: high-pressure homogeneous homogenate is centrifugal through tripod pendulum type batch centrifugal, collects centrifugate.
5. filter: in centrifugate, add talcum powder and each 2kg of diatomite, mix, during to a few achromaticity and clarification of filtrate, collect filtrate through the kieselguhr filter circulating filtration.
6. micro-filtration concentrates: medium filtrate is filtered to reservation liquid measure 45kg through the micro-filtration strainer of series connection 500nm and 200nm ceramic membrane, gets gluey BSP concentrated solution.
7. alcohol precipitation: add ethanol in the concentrated solution, make to contain the alcohol amount and reach 55%, left standstill 6 hours, sedimentation is centrifugal, collects the precipitation of white; Precipitation washing with alcohol 2～3 times, sedimentation is centrifugal, collecting precipitation.
9. vacuum-drying: be layered in the drip pan the precipitation after the washing with alcohol is uniformly thin ,-0.09MPa, be dried to moisture 7% below 65 ℃, pulverize, white BSP 1.6kg.Yield 32%, it is 96% that sulfuric acid-phynol method is measured polysaccharide content.
Below for example understand application of the present invention, under the prerequisite of aim of the present invention and scope, have some modifications on some ins and outs, these modifications are for example equipment tissue, technical qualification, implementation step etc., and can not regard as is limitation of the present invention.
Claims (6)
1. the process combination of BSP preparation, its essential characteristic is: extracting in conjunction with ceramic membrane micro-filtration simmer down to core process, with water based on homogenization is the process combination that the low temperature of solvent prepares the high purity BSP.
2. the process combination of the BSP preparation described of claim 1, the high purity BSP is meant liquid and the solid extract of purity of polysaccharide more than 90%.
3. the process combination of the BSP preparation described of claim 1, homogenization is meant with high pressure homogenizer handles bletilla striata powder and the suspension that extracts water.
4. the process combination of the BSP preparation described of claim 1, micro-filtration concentrates the ceramic membrane filter that is meant filtering accuracy 2000nm～100nm, collects to filter to keep liquid.
5. the process combination of the BSP preparation of claim 1,3 descriptions is extracted water and is meant cryogenic primary reverse osmosis water.
6. the process combination of the BSP preparation described of claim 1,5, low temperature is meant below 30 ℃.
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| CN2009103029373A CN101906169B (en) | 2009-06-05 | 2009-06-05 | Process combination for preparing bletilla polysaccharide |
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| CN2009103029373A CN101906169B (en) | 2009-06-05 | 2009-06-05 | Process combination for preparing bletilla polysaccharide |
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| CN101906169B CN101906169B (en) | 2012-05-23 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150704A (en) * | 2011-01-05 | 2011-08-17 | 赵斌 | Fruit and vegetable fresh-keeping agent and preparation method thereof |
| CN102977220A (en) * | 2012-11-20 | 2013-03-20 | 安徽大学 | Pure physical preparation method of plant and microbial polysaccharide |
| CN109134685A (en) * | 2018-09-30 | 2019-01-04 | 广西益谱检测技术有限公司 | A method of extracting polysaccharide from beautiful millettia root |
| CN110642960A (en) * | 2019-11-07 | 2020-01-03 | 皖西学院 | Double-aqueous-phase extraction method of bletilla striata glucomannan |
| CN111471118A (en) * | 2020-05-22 | 2020-07-31 | 中山火炬职业技术学院 | Preparation method of bletilla striata polysaccharide |
| CN113278085A (en) * | 2021-05-24 | 2021-08-20 | 福建宏泰莱生物科技有限公司 | Non-ethanol precipitated high molecular weight tremella polysaccharide and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100490172B1 (en) * | 2002-05-28 | 2005-05-17 | 주식회사 바이오랜드 | Cosmtic composition containing polysaccharides extracted from Bletilla striata Reichb. fil. |
| CN1709915A (en) * | 2005-06-23 | 2005-12-21 | 南京大学医学院附属鼓楼医院 | Polysaccharide material for promoting cell tactophily and preparation thereof |
| CN101429254B (en) * | 2008-12-05 | 2011-05-04 | 云南植物药业有限公司 | Bletilla striata polysaccharide, preparation method and new uses thereof |
-
2009
- 2009-06-05 CN CN2009103029373A patent/CN101906169B/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150704A (en) * | 2011-01-05 | 2011-08-17 | 赵斌 | Fruit and vegetable fresh-keeping agent and preparation method thereof |
| CN102150704B (en) * | 2011-01-05 | 2013-02-13 | 中山火炬职业技术学院 | Fruit and vegetable fresh-keeping agent and preparation method thereof |
| CN102977220A (en) * | 2012-11-20 | 2013-03-20 | 安徽大学 | Pure physical preparation method of plant and microbial polysaccharide |
| CN109134685A (en) * | 2018-09-30 | 2019-01-04 | 广西益谱检测技术有限公司 | A method of extracting polysaccharide from beautiful millettia root |
| CN110642960A (en) * | 2019-11-07 | 2020-01-03 | 皖西学院 | Double-aqueous-phase extraction method of bletilla striata glucomannan |
| CN110642960B (en) * | 2019-11-07 | 2021-12-10 | 皖西学院 | Double-aqueous-phase extraction method of bletilla striata glucomannan |
| CN111471118A (en) * | 2020-05-22 | 2020-07-31 | 中山火炬职业技术学院 | Preparation method of bletilla striata polysaccharide |
| CN113278085A (en) * | 2021-05-24 | 2021-08-20 | 福建宏泰莱生物科技有限公司 | Non-ethanol precipitated high molecular weight tremella polysaccharide and preparation method thereof |
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| Publication number | Publication date |
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| CN101906169B (en) | 2012-05-23 |
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Denomination of invention: Process combination for preparing bletilla polysaccharide Effective date of registration: 20190920 Granted publication date: 20120523 Pledgee: Bank of China, Limited by Share Ltd, Guangzhou, Panyu branch Pledgor: Zeli Medicine Tech Co., Ltd., Guangzhou Registration number: Y2019440000066 |
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Date of cancellation: 20191120 Granted publication date: 20120523 Pledgee: Bank of China, Limited by Share Ltd, Guangzhou, Panyu branch Pledgor: Zeli Medicine Tech Co., Ltd., Guangzhou Registration number: Y2019440000066 |
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