CN101899404B - Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether - Google Patents
Acinetobacter calcoaceticus and application thereof in degrading decabromodiphenyl ether Download PDFInfo
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- CN101899404B CN101899404B CN2010100192361A CN201010019236A CN101899404B CN 101899404 B CN101899404 B CN 101899404B CN 2010100192361 A CN2010100192361 A CN 2010100192361A CN 201010019236 A CN201010019236 A CN 201010019236A CN 101899404 B CN101899404 B CN 101899404B
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- acinetobacter calcoaceticus
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Abstract
The invention discloses acinetobacter calcoaceticus DB-3 and application thereof in degrading decabromodiphenyl ether. The acinetobacter calcoaceticus is preserved in the China Center for Type Culture Collection (CCTCC) on November 27th, 2009, and the collection number is CCTCC No:M 209287. The acinetobacter calcoaceticus has relatively strong degradation capability for the decabromodiphenyl ether and can debrominate the decabromodiphenyl ether to produce dissociative bromonium ions. After the biological degradation of the acinetobacter calcoaceticus, the decabromodiphenyl ether does not produce highly-toxic secondary pollutants such as polybrominated dibenzo-p-dioxin, polybrominated dibenzofuran, low-brominated diphenyl ether and the like. The acinetobacter calcoaceticus strain can be used for controlling the decabromodiphenyl ether pollution in the environment so as to provide low-cost and high-efficiency decabromodiphenyl ether degrading bacteria without secondary pollution for treating the decabromodiphenyl ether in chemical industrial sludge or wastewater.
Description
Technical field:
The invention belongs to the microorganism Application Areas, be specifically related to Acinetobacter calcoaceticus (Acinetobacter calcoaceticusDB-3) and the application in degrading decabromodiphenyl ether thereof.
Background technology:
Decabromodiphenyl oxide (BDE2209 or DeBDE) is a class Poly Brominated Diphenyl Ethers that has the call on the market, as a kind of bromide fire retardant (brominated flame retardants, BFRs), be widely used as the additive of impact resistant polystyrene, polyester, polymeric amide, textiles and electronic product.In recent years, the demand of decabromodiphenyl oxide constantly increases, and according to statistics, the whole world in 1999 accounts for 81% of Poly Brominated Diphenyl Ethers aggregate demand the demand of BDE2209, and calendar year 2001 is 83%.Till calendar year 2001, China's fire retardant ultimate production is about 1.5 * 10
5T, and the sales volume of BDE2209 has reached 1.35 * 10
4T.The decabromodiphenyl ether content manifests outstanding growing trend in the physical environment of the whole world.Brominated flame-retardant has the water of being insoluble in, and the long-distance migration ability is difficult to physics and chemistry characteristics such as biological degradation.Poly Brominated Diphenyl Ethers has tangible biological accumulation characteristic simultaneously, shows harm such as carinogenicity, neurotoxicity, internal secretion interference toxicity, causing property of immunologic function disorder on the physiology.After decabromodiphenyl oxide is discharged in the environment, through processes such as photodissociation, pyrolytic decomposition, biology and microbiological deteriorations, can be converted into the biphenyl ether of many bromines dibenzodioxin English, many bromines diphenylene-oxide and low bromo, these degradation products are easier to enter organism, also causes stronger toxic action.The decabromodiphenyl oxide that exists in the environment becomes a kind of ecological safety gradually to be threatened.Therefore, must excavation can be efficiently, the method for safe disposal decabromodiphenyl oxide.
Biological degradation is the microbiological degradation material that utilizes nature to exist, and can not cause negative impact to environment.The bacterial strain with decabromodiphenyl oxide degradation capability of report includes White rot fungi at present, Rhodococcus jostii RHA1 and Burkholderia xenovorans LB400 etc., but their degradation rates are very low, and function is not strong.Therefore, excavation can be efficiently, the function stem of safe disposal decabromodiphenyl oxide has great importance.
Up to the present, the bacterial strain of not reporting acinetobacter (Acinetobacter) has the ability of degrading decabromodiphenyl ether.
Summary of the invention:
The purpose of this invention is to provide a kind of Acinetobacter calcoaceticus (Acinetobacter calcoaceticus DB-3) and the application in degrading decabromodiphenyl ether thereof, this Acinetobacter calcoaceticus (Acinetobacter calcoaceticus DB-3) can be efficiently, the safe disposal decabromodiphenyl oxide, can not produce the highly toxic secondary pollutants such as biphenyl ether of many bromines dibenzodioxin English, many bromines diphenylene-oxide and low bromo.
Acinetobacter calcoaceticus DB-3 of the present invention obtains from the separation of Gui Yu riverbed, Shantou, Guangdong bed mud, purifying:
The physical and chemical parameter of this bacterium is as follows:
1, the morphological character of thalline:
A. adopt conventional bacterium Physiology and biochemistry authentication method and electron microscope observation, Acinetobacter calcoaceticusDB-3 bacterial strain is a Gram-negative, the club shape, and the thalline size is 1～1.5 μ m * 1.5～2.5 μ m microns, no gemma.
B. after cultivating 24h on the LB solid medium flat board, colonial morphology is circular, and smooth opaque, colony diameter is the 1.5-2.0 millimeter.
2, the main physicochemical property of Acinetobacter calcoaceticus DB-3:
Growth temperature 20-40 ℃ of Acinetobacter calcoaceticus DB-3 bacterial strain, pH scope 6-9, aerobic growth, oxidase negative, the catalase positive.
3, the main hereditary feature of Acinetobacter calcoaceticus DB-3:
16S rRNA order-checking to Acinetobacter calcoaceticus DB-3, BLAST compares discovery, the existing type strain of the 16S rRNA gene order of Acinetobacter calcoaceticus DB-3 of the present invention and Acinetobacter calcoaceticus has higher similarity, and wherein the sequence similarity with Acinetobacter johnsonii DSM 6963 is 84%.
Comprehensive above-mentioned physio-biochemical characteristics, 16S rRNA gene order result, Acinetobacter calcoaceticusDB-3 of the present invention should belong to Acinetobacter calcoaceticus (Acinetobacter calcoaceticus), called after Acinetobacter calcoaceticusDB-3.
The inventor confirms that by experiment Acinetobacter calcoaceticus DB-3 of the present invention has decabromodiphenyl oxide debrominate ability, has stronger degradation capability to decabromodiphenyl oxide.
Acinetobacter calcoaceticus DB-3 bacterial strain of the present invention has stronger degradation capability to decabromodiphenyl oxide, can be to the decabromodiphenyl oxide debrominate, and produce dissociative bromonium ions.Therefore can not produce the highly toxic secondary pollutants such as biphenyl ether of many bromines dibenzodioxin English, many bromines diphenylene-oxide and low bromo after decabromodiphenyl oxide is handled through this bacterium biological degradation.So this bacterial strain can be used for environment administer decabromodiphenyl oxide contaminated, thereby the decabromodiphenyl oxide degradation bacteria that provides a kind of low cost, high-level efficiency that secondary pollution does not take place for the processing of decabromodiphenyl oxide in chemical engineering sludge or the waste water.
Acinetobacter calcoaceticus DB-3 of the present invention was on November 27th, 2009, and (CCTCC, the address: Chinese Wuhan City Wuhan University), its deposit number is CCTCC NO:M 209287 to be preserved in Chinese typical culture collection center.
Embodiment:
One, the separation and the evaluation of Acinetobacter calcoaceticus DB-3 bacterial strain
1, the separation of Acinetobacter calcoaceticus DB-3 bacterial strain:
Substratum:
Basis salt culture medium: contain KH in every liter of substratum
2PO
42.93g, Na
2HPO
48.87g, NH
4Cl1.0g, NaCl0.5g, MgSO
45g, CaCl
22H
2O0.15g, micro-salts solution 2mL, surplus is a water.
Trace element salts solution: contain Na in every liter of micro-salts solution
2MoO
430mg, ZnCl
250mg, CuCl
22H
2O10mg, H
3BO
331mg, MnCl
25H
2O 30mg, NiCl
26H
2O 20mg, CoCl
26H
2O 36mg, surplus is a water.
Acinetobacter calcoaceticus DB-3 bacterial strain of the present invention is to get through separation, purifying from Gui Yu riverbed, Shantou, Guangdong bed mud.
Its separation purification method is as follows:
Getting 1g riverbed bed mud is placed on 100ml and is added with decabromodiphenyl oxide 2.0mg/L, Sodium.alpha.-hydroxypropionate 1.0g/L, enrichment culture in the basic salt culture medium of yeast extract 0.1g/L.The enrichment culture process is by the change in concentration indication concentration effect of bromide anion in the chromatography of ions monitoring nutrient solution, enrichment culture is earlier at 30 ℃, lucifuge leaves standstill cultivated 14 days, monitoring out tangible bromide anion discharges, be transferred to then and contain decabromodiphenyl oxide 2.0mg/L, Sodium.alpha.-hydroxypropionate 2.0g/L continues in the basic salt culture medium of yeast extract 0.05g/L at 30 ℃, and lucifuge leaves standstill cultivated 14 days.Getting mixed bacterial liquid then in the nutrient solution of enrichment culture of last step is applied to add and contains Sodium.alpha.-hydroxypropionate 2.0g/L solid basis salt culture medium (containing 20g/L agar) and cultivate in 30 ℃ of incubators, therefrom the single bacterium colony of picking advantage carries out debrominate degraded screening, purifying obtains Acinetobacter calcoaceticus DB-3 bacterial strain at last.2, Acinetobacter calcoaceticus DB-3 strain characteristics:
(1) morphological character of thalline:
A. adopt conventional bacterium Physiology and biochemistry authentication method and electron microscope observation, Acinetobacter calcoaceticusDB-3 bacterial strain is a Gram-negative, the club shape, and the thalline size is 1～1.5 μ m * 1.5～2.5 μ m microns, no gemma.
B. after cultivating 24h on the LB solid medium flat board, colonial morphology is circular, and smooth opaque, colony diameter is the 1.5-2.0 millimeter.
(2) the main physicochemical property of Acinetobacter calcoaceticus DB-3:
Growth temperature 20-40 ℃ of Acinetobacter calcoaceticus DB-3 bacterial strain, pH scope 6-9, aerobic growth, oxidase negative, the catalase positive.
(3) the main hereditary feature of Acinetobacter calcoaceticus DB-3:
The condition that conditioned disjunction described in the concrete grammar reference molecule cloning experimentation guide (New York:Cold Spring Harbor Laboratory Press, 2001) is advised according to manufacturer.
Acinetobacter calcoaceticus DB-3 molecular classification of the present invention status:
Adopt DNA extracting method extraction Acinetobacter calcoaceticus DB-3 bacteria total DNA in a small amount, adopt 16S rRNA universal primer 27F and 1522r its 16S rRNA gene that increases, the PCR product is directly checked order, its sequence is shown in SEQ ID NO.1, with the 16S rRNA gene order input GenBank that obtains, by blast program all sequences in the database is compared analysis, the 16S rRNA gene order and the existing type strain of Acinetobacter calcoaceticus that found that Acinetobacter calcoaceticus DB-3 of the present invention have higher similarity, and wherein the sequence similarity with Acinetobacter johnsoniiDSM 6963 is 84%.
Comprehensive above-mentioned physio-biochemical characteristics, 16S rRNA gene order result, Acinetobacter calcoaceticusDB-3 of the present invention should belong to Acinetobacter calcoaceticus (Acinetobacter calcoaceticus), called after Acinetobacter calcoaceticusDB-3.
Acinetobacter calcoaceticus DB-3 of the present invention was on November 27th, 2009, and (CCTCC, the address: Chinese Wuhan City Wuhan University), its deposit number is CCTCC NO:M 209287 to be preserved in Chinese typical culture collection center.
Embodiment one:
Acinetobacter calcoaceticus DB-3 is the debrominate degraded test of the decabromodiphenyl oxide of 10mg/L to concentration:
Degraded basic medium prescription: contain peptone 1g in every liter of substratum, yeast extract 0.5g, KH
2PO
42.93g, Na
2HPO
48.87g, NH
4Cl 1.0g, NaCl 0.5g, MgSO
45g, CaCl
22H
2O 0.15g, surplus is a water.
1, preparation contains the degradation experiment substratum of decabromodiphenyl oxide 10mg/L:
Add 300mg/L methylene dichloride dissolved decabromodiphenyl oxide storage liquid 1ml in the 100ml triangular flask, lucifuge is treated the methylene dichloride volatilization, adds 30ml degraded basic medium then in triangular flask, obtains 30ml degradation experiment substratum after shaking up.
Be the prescription of degradation experiment substratum: contain decabromodiphenyl oxide 10mg in every liter of substratum, peptone 1g, yeast extract 0.5g, KH
2PO
42.93g, Na
2HPO
48.87g, NH
4Cl 1.0g, NaCl 0.5g, MgSO
45g, CaCl
22H
2O 0.15g, surplus is a water.
2, the pure bacterium of Acinetobacter calcoaceticus DB-3 of the present invention being drawn the LB plate cultivates, then from solid LB flat board picking list colony inoculation to 30ml degradation experiment substratum, 30 ℃, 150 rev/mins of lucifuges are cultivated, after cultivating 48h, get nutrient solution, utilize bromide ion concentration in the ion chromatography nutrient solution, three parallel sample are done in experiment.
3, the drafting of bromide anion bioassay standard curve and mensuration:
Prepare 0.00,100,250,500,750,1000 μ g/L Sodium Bromide normal gradients solution respectively with the mini-Q sterilized water of 18.2m Ω, carry out stratographic analysis with wearing peace ICS-1500 type chromatography of ions, analytical column AS19, analytic sample amount 50 μ L, analysis time 30min, concrete data are as shown in table 1:
Table 1
| The Sodium Bromide concentration of standard solution | 0.00 | 100 | 250 | 500 | 750 | 1000 | 1500 |
| Measure bromide ion concentration | 0.000 | 98.68 | 252.45 | 499.82 | 747.76 | 1001.29 | 1498.65 |
Draw typical curve equation: y=0.0027x-0.0027 thus.R
2=1.0, x is bromide ion concentration (the μ g/L of unit), and y is the long-pending peak area (μ S*min) of bromide anion.
4, Acinetobacter calcoaceticus DB-3 is to the mensuration of decabromodiphenyl oxide debrominate degraded:
From three parallel sample, get the nutrient solution of 2mL respectively through the above-mentioned cultivation of 48h, with the aseptic membrane filtration of 0.22 μ m, filtered liquid is collected refined solution through C18 solid phase extraction column purifying, get and wear the peace ion chromatography after 0.5mL is diluted to 6mL, last sample amount of analysis is 500 μ L.By above-mentioned typical curve Equation for Calculating, can get behind the 48h that bromide ion concentration is respectively 300.0 μ g/L in the Acinetobactercalcoaceticus DB-3 nutrient solution, 227.0 μ g/L, 257.4 μ g/L, mean value are 261.5 μ g/L.
Embodiment two:
Acinetobacter calcoaceticus DB-3 is the debrominate degraded test of the decabromodiphenyl oxide of 10mg/L to concentration:
1, preparation contains the degradation experiment substratum of decabromodiphenyl oxide 10mg/L:
The degradation experiment substratum of present embodiment is identical with the degradation experiment substratum of embodiment one.
2, the pure bacterium of Acinetobacter calcoaceticus DB-3 of the present invention being drawn the LB plate cultivates, then from the dull and stereotyped picking list of solid LB colony inoculation to 30ml degradation experiment substratum, in 30 ℃, 150 rev/mins of lucifuges are cultivated, after cultivating 60h, get nutrient solution, utilize bromide ion concentration in the ion chromatography nutrient solution, three parallel sample are done in experiment.
3, the mensuration of Acinetobacter calcoaceticus DB-3 degradation rate:
From three parallel sample, get the nutrient solution of 2mL respectively through the above-mentioned cultivation of 60h, with the aseptic membrane filtration of 0.22 μ m, filtered liquid is collected refined solution through C18 solid phase extraction column purifying, get and wear the peace ion chromatography after 0.5mL is diluted to 6mL, last sample amount of analysis is 500 μ L.According to the typical curve Equation for Calculating of embodiment one, can get behind the 60h that bromide ion concentration is respectively 498.3 μ g/L in the Acinetobactercalcoaceticus DB-3 nutrient solution, 420.0 μ g/L, 389.7 μ g/L, mean value are 436.0 μ g/L.
Embodiment three:
Acinetobacter calcoaceticus DB-3 is the debrominate degraded test of the decabromodiphenyl oxide of 10mg/L to concentration:
1, preparation contains the degradation experiment substratum of decabromodiphenyl oxide 10mg/L:
The degradation experiment substratum of present embodiment is identical with the degradation experiment substratum of embodiment one.
2, the pure bacterium of Acinetobacter calcoaceticus DB-3 of the present invention is drawn the conventional cultivation of LB plate, picking list bacterium is seeded to the 30ml degradation experiment substratum from solid LB flat board then, 30 ℃, after 150 rev/mins of lucifuges are cultivated 72h, get nutrient solution, utilize bromide ion concentration in the ion chromatography nutrient solution, three parallel sample are done in experiment.
3, the mensuration of Acinetobacter calcoaceticus DB-3 degradation rate:
From three parallel sample, get the nutrient solution of 2mL respectively through the above-mentioned cultivation of 72h, with the aseptic membrane filtration of 0.22 μ m, filtered liquid is collected refined solution through C18 solid phase extraction column purifying, get and wear the peace ion chromatography after 0.5mL is diluted to 6mL, last sample amount of analysis is 500 μ L.Press the typical curve Equation for Calculating of embodiment one, can get that bromide ion concentration is respectively 701.7 μ g/L in the 72hAcinetobactercalcoaceticus DB-3 nutrient solution, 550.4 μ g/L, 653.5 μ g/L, mean value are 635.2 μ g/L
Claims (2)
1. Acinetobacter calcoaceticus Acinetobacter calcoaceticus DB-3, its preserving number is: CCTCC NO:M 209287.
2. the application of the described Acinetobacter calcoaceticus Acinetobacter of claim 1 calcoaceticus DB-3 in degrading decabromodiphenyl ether.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695332A (en) * | 2013-10-18 | 2014-04-02 | 环境保护部华南环境科学研究所 | Novel diesel oil pollution type place microbe degrading bacteria |
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| CN102181384B (en) * | 2011-02-25 | 2012-11-28 | 中国科学院烟台海岸带研究所 | Acinetobacter calcoaceticus T32 capable of metabolizing furazolidone and application thereof |
| CN102363756B (en) * | 2011-10-08 | 2013-01-23 | 广东省微生物研究所 | Lysinibacillus sp. Gy32 and application thereof |
| CN103409349B (en) * | 2013-08-02 | 2015-04-22 | 东华大学 | Leifsonia shinshuensis and degradation method of leifsonia shinshuensis to decabromodiphenyl ether |
| CN103642713B (en) * | 2013-11-11 | 2015-12-02 | 华南理工大学 | A kind of dominant microflora for degrading decabromodiphenyl ether waste water and preparation method thereof |
| CN107058156B (en) * | 2016-12-27 | 2020-01-31 | 北京市农林科学院 | Tetracycline antibiotic degrading strain, microbial inoculum containing strain and application thereof |
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| CN113717260B (en) * | 2021-08-26 | 2023-05-23 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Polybrominated diphenyl ether receptive protein and whole-cell microbial sensor constructed by polybrominated diphenyl ether receptive protein |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739822A (en) * | 2005-09-22 | 2006-03-01 | 中国科学院生态环境研究中心 | The method of nanometer iron powder fast polybrominated biphenyl ether degrading |
-
2010
- 2010-01-05 CN CN2010100192361A patent/CN101899404B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739822A (en) * | 2005-09-22 | 2006-03-01 | 中国科学院生态环境研究中心 | The method of nanometer iron powder fast polybrominated biphenyl ether degrading |
Non-Patent Citations (3)
| Title |
|---|
| Gerecke AC等.Anaerobic degradation of decabromodiphenyl ether.《Environ Sci Technol》.2005,第39卷(第4期),1078-1083. * |
| Juan Zhou等.Effect of Tween 80 and b-cyclodextrin on degradation of decabromodiphenyl ether (BDE-209) by White Rot Fungi.《chemosphere》.2007,第70卷172–177. * |
| 丁娟等.多溴联苯醚好氧生物降解研究.《环境科学》.2008,第29卷(第11期),3179-3184. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695332A (en) * | 2013-10-18 | 2014-04-02 | 环境保护部华南环境科学研究所 | Novel diesel oil pollution type place microbe degrading bacteria |
| CN103695332B (en) * | 2013-10-18 | 2016-03-02 | 环境保护部华南环境科学研究所 | A kind of contaminated by diesel oil type place microbiological deterioration bacterium |
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