CN101892288A - Method for testing putrefaction capacity of putrefactive bacteria of fish - Google Patents
Method for testing putrefaction capacity of putrefactive bacteria of fish Download PDFInfo
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- CN101892288A CN101892288A CN2009100514377A CN200910051437A CN101892288A CN 101892288 A CN101892288 A CN 101892288A CN 2009100514377 A CN2009100514377 A CN 2009100514377A CN 200910051437 A CN200910051437 A CN 200910051437A CN 101892288 A CN101892288 A CN 101892288A
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- fish
- spoilage organism
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Abstract
The invention discloses a method for testing the putrefaction capacity of putrefactive bacteria of fish, relates to fish preservation technology, and aims to provide a method for testing and quantitatively analyzing the putrefaction capacity of the putrefactive bacteria of fish. The technical scheme comprises the steps of preparing pure bacterial liquid of the putrefactive bacteria, preparing aseptic fish blocks, inoculating, and analyzing the growth rate of the putrefactive bacteria and the generation rate of putrefactive metabolic product. The method is characterized in that: the prepared aseptic fish blocks are soaked in the pure bacterial liquid of the putrefactive bacteria to be tested, the inoculated fish blocks are stored at the temperature of (5+/-0.1) DEG C, and the sensory evaluation, total volatile basic nitrogen (TVBN) test and to-be-tested putrefactive bacteria counting are respectively carried out on the inoculated fish blocks every 24 hours; and the putrefaction capacity of the putrefactive bacteria is represented by the quantity of the putrefactive metabolic product (TVBN) generated by unit putrefactive bacteria inoculated into the fish blocks, and a TVBN yield factor (YTVBN/CFU) of the sensory putrefactive point is taken as a quantitative index of the putrefaction capacity of the putrefactive bacteria.
Description
Technical field
The present invention relates to the preservation technique of fish.
Background technology
The fresh fishes corruption shows that mainly some bacterial growth and metabolism generate amine, sulfide, aldehyde, ketone, ester, organic acid etc., produces unpleasant odor and taste, makes fish products become unacceptable on the sense organ.Though the first meeting of fish body is subjected to multiple microbiological contamination, have only the part bacterium to participate in decay process.The flora of these suitable specific environment existence and breeding and generation putrefactive odor and peculiar smell meta-bolites is exactly the specific spoilage organism of this fish products.Specific spoilage organism other bacteriums of growth fraction in storage are fast, and corrupt ability is strong.Some bacteriums may generate and corrupt relevant meta-bolites, but the corrupt meta-bolites of its generation is not significant, and a little less than the promptly corrupt ability, these bacteriums are not specific spoilage organism just.Therefore measuring with analyzing the corrupt ability of fish products spoilage organism is specific spoilage organism of researching fish and the indispensable basis of exploitation fish preservation technique.
Summary of the invention
The technical issues that need to address of the present invention provide the measuring method of the corrupt ability of a kind of fish spoilage organism.
Technical scheme of the present invention comprises the pure bacterium liquid preparation of spoilage organism, the preparation of aseptic fish piece, inoculation and spoilage organism growth velocity and the analysis of corrupt meta-bolites generating rate, it is characterized in that the aseptic fish piece of preparation is immersed in the pure bacterium liquid of spoilage organism, to inoculate back fish piece and under (5 ± 0.1) ℃ temperature, preserve, and every about 24 hours, get inoculation fish piece and do sensory evaluation, total volatile basic nitrogen (TVBN) mensuration, spoilage organism to be determined counting respectively; The corrupt ability of spoilage organism is represented with the amount of the corrupt meta-bolites (TVBN) that the unit spoilage organism that is inoculated in the aseptic fish piece produces, with the TVBN yield factor (Y of the corrupt point of sense organ
TVBN/CFU) as the quantitative target of the corrupt ability of spoilage organism; The quantitative target of the corrupt ability of spoilage organism is calculated as follows:
The method that provides and the result of mensuration are provided for outstanding feature of the present invention and marked improvement, for the specific spoilage organism of researching fish and exploitation fish preservation technique provide science data.
Embodiment
The mensuration of the corrupt ability of fish spoilage organism is carried out according to the following steps:
1. the preparation of the pure bacterium liquid of spoilage organism
Choose spoilage organism bacterial strain to be determined, on nutrient agar, rule, cultivate 24～48h for 25 ℃.Get a ring lawn and be inoculated in the 300mL nutrient broth (at 500mL circular cone flask), 25 ℃ of aerobic static cultivation 12～18h make bacterial concentration reach 10
8About cfu/g, bacterium liquid is centrifugal, outwell supernatant liquor, with sterilization 0.1% peptone physiological saline bacterium liquid being diluted to spoilage organism bacterium number is 10
6Pure bacterium liquid about cfu/g is used for inoculation.
2. the preparation of aseptic fish piece
Choose the fish that are fit to spoilage organism growth to be determined, select for use live fish promptly to kill or catch the back iced storage time to be no more than 36 hours fresh fish.Fish is cleaned, scaled, remove fin, remove internal organ, clean.Fish is placed on the chopping block that is covered with preservative film, after with thieving paper the water on the fish body being blotted, with 75% alcohol wipe fish body.Cut the pollution-free flesh of fish of ridge portion with the sterilization scalper, carefully remove peeling fish skin.The flesh of fish that cuts is cut into the fish piece of size, form unanimity.Measure the total number of bacterial colony of fish piece, total number of bacterial colony must not be greater than 10
2Cfu/g.
3. inoculation
Aseptic fish piece with preparation is tiled in the sterilizing stainless steel basket, immerses in the pure bacterium liquid of spoilage organism to be determined, and flooding waterlogs out after about 10 seconds drains.Measure fish piece spoilage organism bacterium to be determined number, the numerical control of spoilage organism bacterium is built in 10
4About cfu/g.
4. storage
Inoculation back fish piece is placed in the enamel tray of sterilization, covers the lid of leakage hole, put into the high precision low temperature incubator, control reserve temperature (5 ± 0.1) ℃.Every about 24 hours, get inoculation fish piece and do sensory evaluation, total volatile basic nitrogen (TVBN) mensuration, spoilage organism to be determined counting respectively.
5. corrupt ability quantitative target
The corrupt ability of spoilage organism is represented with the amount of the corrupt meta-bolites (TVBN) that the unit spoilage organism that is inoculated in the aseptic fish piece produces, with the TVBN yield factor (Y of the corrupt point of sense organ
TVBN/CFU) as the quantitative target of the corrupt ability of spoilage organism.The quantitative target of the corrupt ability of spoilage organism is calculated as follows:
Claims (3)
1. the measuring method of the corrupt ability of fish spoilage organism, comprise the pure bacterium liquid preparation of spoilage organism, the preparation of aseptic fish piece, inoculation and spoilage organism growth velocity and the analysis of corrupt meta-bolites generating rate, it is characterized in that the aseptic fish piece of preparation is immersed in the pure bacterium liquid of spoilage organism, to inoculate back fish piece and under (5 ± 0.1) ℃ temperature, preserve, and every about 24 hours, get inoculation fish piece and do sensory evaluation, total volatile basic nitrogen (TVBN) mensuration, spoilage organism to be determined counting respectively; The corrupt ability of spoilage organism is represented with the amount of the corrupt meta-bolites (TVBN) that the unit spoilage organism that is inoculated in the aseptic fish piece produces, with the TVBN yield factor (Y of the corrupt point of sense organ
TVBN/CFU) as the quantitative target of the corrupt ability of spoilage organism, the corrupt ability quantitative target of spoilage organism is calculated as follows:
2. the measuring method of the corrupt ability of fish spoilage organism according to claim 1, it is characterized in that choosing the fish that are fit to spoilage organism growth to be determined, select for use live fish promptly to kill or catch the back iced storage time and be no more than 36 hours fresh fish, fish is cleaned, scale, remove fin, remove internal organ, clean, after the water on the fish body is blotted, with 75% alcohol wipe fish body, cut the pollution-free flesh of fish of ridge portion with the sterilization scalper, remove peeling fish skin, the flesh of fish is flitched, measure the total number of bacterial colony of fish piece, total number of bacterial colony must not be greater than 10
2Cfu/g.
3. the measuring method of the corrupt ability of fish spoilage organism according to claim 1 and 2, the bacterium number of the pure bacterium liquid of spoilage organism that it is characterized in that being used for aseptic fish piece inoculation is 10
6About cfu/g, the spoilage organism bacterium number of inoculation back fish piece is 10
4About cfu/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100514377A CN101892288A (en) | 2009-05-18 | 2009-05-18 | Method for testing putrefaction capacity of putrefactive bacteria of fish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100514377A CN101892288A (en) | 2009-05-18 | 2009-05-18 | Method for testing putrefaction capacity of putrefactive bacteria of fish |
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| CN101892288A true CN101892288A (en) | 2010-11-24 |
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| CN2009100514377A Pending CN101892288A (en) | 2009-05-18 | 2009-05-18 | Method for testing putrefaction capacity of putrefactive bacteria of fish |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108796030A (en) * | 2018-05-29 | 2018-11-13 | 浙江大学 | A kind of aquatic products microorganism causes the evaluation method of rotten ability |
| CN113702121A (en) * | 2021-08-04 | 2021-11-26 | 大连工业大学 | Preparation method of sample for evaluating fish quality |
-
2009
- 2009-05-18 CN CN2009100514377A patent/CN101892288A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108796030A (en) * | 2018-05-29 | 2018-11-13 | 浙江大学 | A kind of aquatic products microorganism causes the evaluation method of rotten ability |
| CN113702121A (en) * | 2021-08-04 | 2021-11-26 | 大连工业大学 | Preparation method of sample for evaluating fish quality |
| CN113702121B (en) * | 2021-08-04 | 2023-11-21 | 大连工业大学 | Preparation method of sample for evaluating fish quality |
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Application publication date: 20101124 |