CN101884569B - Smoke exposure device used for establishing chronic obstructive pulmonary disease model - Google Patents
Smoke exposure device used for establishing chronic obstructive pulmonary disease model Download PDFInfo
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- CN101884569B CN101884569B CN201010193138XA CN201010193138A CN101884569B CN 101884569 B CN101884569 B CN 101884569B CN 201010193138X A CN201010193138X A CN 201010193138XA CN 201010193138 A CN201010193138 A CN 201010193138A CN 101884569 B CN101884569 B CN 101884569B
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Abstract
The invention discloses a smoke exposure device used for establishing a chronic obstructive pulmonary disease model. The smoke exposure device mainly comprises a flow regulating device, a peristaltic pump and a closed box, wherein the flow regulating device comprises a smoke inlet pipe, an air inlet pipe, a smoke outlet pipe, an air outlet pipe, a smoke flow regulator and an air flow regulator; the smoke flow regulator is communicated with the smoke inlet pipe and the smoke outlet pipe respectively; the air flow regulator is communicated with the air inlet pipe and the air outlet pipe respectively; the smoke outlet pipe, the air outlet pipe and an air duct are communicated with one another; and the air duct is communicated with the closed box and is arranged inside a pump bed of the peristaltic pump. The smoke exposure device has the advantages of precisely regulating the smoke flow and the ratio of the smoke flow to air, facilitating quantitative analysis, improving the stability, reliability and repeatability of an experiment, and effectively avoiding the suffocation death of experimental animals, the cigarette smoke inhalation of experiment operators and atmospheric pollution, along with simple structure, convenient manufacturing and low cost.
Description
Technical field
The present invention relates to a kind of smoke exposure device that is used to set up chronic obstructive pulmonary disease model, belong to basic medical research experiment apparatus field.
Background technology
Clear and definite already, smoking is the topmost paathogenic factor of human chronic obstructive pulmonary disease.Simulate this pathogenic process, expose the research of generally acknowledging and being widely used in chronic obstructive pulmonary disease pathogenesis and clinical prevention that the emphysema animal model that the laboratory animal passive smoking is brought out has obtained vast researcher by smog.
At present, domestic researcher adopts the sootiness device to make up the animal chronic obstructive pulmonary disease model mostly, reported method is similar, can simply be described below: airtight container is separated two parts up and down with shelf with holes, laboratory animal is placed on the shelf, below shelf, lights medicated cigarette then and produce smog.This class device can't regulate smog flow and with the ratio of air, exist the modelling instability, repeatable relatively poor, very easily cause a large amount of smog of laboratory animal death by suffocation and experiment operator to expose and shortcomings such as air pollution.The external improved device of having reported many outfit exhaust equipments, part scholar has adopted macrolon cage in experiment, and this device is equipped with a rodent inhaler, can regulate the ratio and the flow of cigarette and air; A part of in addition scholar has then designed the another kind of quantitative smoke exposure device of mainly being made up of inhaler and peristaltic pump.Although these improved device have the commercially available prod, cost an arm and a leg, be difficult to apply at the most of laboratorys of China.
Summary of the invention
Technical problem to be solved by this invention provides a kind of smoke exposure device simple, that chronic obstructive pulmonary disease model is set up in low being used to of cost of making.
The present invention solves the technical scheme that its technical problem takes: this smoke exposure device that is used to set up chronic obstructive pulmonary disease model mainly comprises flow regulator, peristaltic pump and closed box, described flow regulator comprises the smog air inlet pipe, air intake duct, the smog escape pipe, the air escape pipe, smog flow-controlling meter and both air flow modulation meter, the smog flow-controlling meter respectively with the smog air inlet pipe, the smog escape pipe is communicated with, other and the air intake duct of both air flow modulation score, the air escape pipe is communicated with, the smog escape pipe, air escape pipe and airway are interconnected, described airway is communicated with closed box, and described airway places in the pump line bed of peristaltic pump.
Further, the present invention also comprises the smog shielded box, and described flow regulator, peristaltic pump and closed box place in the smog shielded box, and the top of described smog shielded box is provided with air vent, and the smog shielded box is being provided with action pane near smog air inlet pipe place.
Compared with prior art, the invention has the beneficial effects as follows: (1) is simple in structure, easy to make, and is with low cost; (2) can accurately regulate and control the smog flow and with the ratio of air, play quantitative effect, thereby improve stability, reliability and the repeatability of experiment; (3) effectively avoid suction and the atmospheric pollution of the death by suffocation, experiment operator of laboratory animal to smoke from cigarette.
Description of drawings
Fig. 1 is the structural representation of a kind of embodiment of apparatus of the present invention;
Among the figure, 1. smog air inlet pipe, 2. smog flow-controlling meter, 3. smog escape pipe, 4. air intake duct, 5. both air flow modulation meter, 6. air escape pipe, 7. flow regulator, 8. airway, 9. peristaltic pump, 10. pump line bed, 11. closed boxs, 12. smog shielded boxs.
Fig. 2 is the bronchus pathology figure of normal mouse.
Fig. 3 is to use the mouse bronchial pathology figure after apparatus of the present invention expose mice enforcement smog.
Fig. 4 is the alveolar pathology figure of normal mouse.
Fig. 5 is to use the mice alveolar pathology figure after apparatus of the present invention expose mice enforcement smog.
The specific embodiment
In Fig. 1, flow regulator 7 comprises smog air inlet pipe 1, air intake duct 4, smog escape pipe 3, air escape pipe 6, smog flow-controlling meter 2 and both air flow modulation meter 5.The two ends of smog flow-controlling meter 2 are communicated with smog air inlet pipe 1 and smog escape pipe 3 respectively, the two ends of both air flow modulation meter 5 are communicated with air intake duct 4 and air escape pipe 6 respectively, can accurately regulate and control thus the smog flow and with the ratio of air, be convenient to carry out quantitative analysis, thereby improve stability, reliability and the repeatability of experiment, effect is remarkable.In addition, smog escape pipe 3, air escape pipe 6 and airway 8 are interconnected, and airway 8 is communicated with closed box 11, and airway 8 places in the pump line bed 10 of peristaltic pump 9.
Below illustrate the result of use of apparatus of the present invention.It is the peristaltic pump of BT300-2J that the present invention can adopt model, is made by industrial Science and Technology Ltd. of the auspicious nation in Beijing.20 male C57b1/6 mices are divided into 2 groups at random: 10 of matched groups, raised 3 months; 10 of model group place in the closed box 11.It is 30ml/ minute that the rotating speed of regulating peristaltic pump 9 makes the smog flow, regulating smog flow-controlling meter 2 and both air flow modulation meter 5, to make the ratio of smog and air be 1: 8; 1 time/day, continue 90 minutes at every turn, 5 days/week, the model group mice is exposed 3 months.The pathological change of mouse bronchial alveolar is observed in HE dyeing.Found that, with Fig. 2 and the matched group comparison of not carrying out the smog exposure shown in Figure 4, as Fig. 3 and shown in Figure 5, matter granuloma between the model group mice occurs after smog exposes 3 months, the necrosis of bronchial mucosa epithelium comes off, glandular hyperplasia hypertrophy, goblet cell hypertrophy, smooth muscle obviously thicken, mucosa down and adventitia collagen fiber hypertrophy, emphysema pathological changes expanded range, degree increase the weight of.Show that thus apparatus of the present invention have good experiment stability, reliability and repeatability.
Claims (2)
1. smoke exposure device that is used to set up chronic obstructive pulmonary disease model, it is characterized in that: comprise flow regulator, peristaltic pump and closed box, described flow regulator comprises the smog air inlet pipe, air intake duct, the smog escape pipe, the air escape pipe, smog flow-controlling meter and both air flow modulation meter, the smog flow-controlling meter respectively with the smog air inlet pipe, the smog escape pipe is communicated with, other and the air intake duct of both air flow modulation score, the air escape pipe is communicated with, the smog escape pipe, air escape pipe and airway are interconnected, described airway is communicated with closed box, and described airway places in the pump line bed of peristaltic pump.
2. the smoke exposure device that is used to set up chronic obstructive pulmonary disease model according to claim 1, it is characterized in that: also comprise the smog shielded box, described flow regulator, peristaltic pump and closed box place in the smog shielded box, the top of described smog shielded box is provided with air vent, and the smog shielded box is being provided with action pane near smog air inlet pipe place.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010193138XA CN101884569B (en) | 2010-06-04 | 2010-06-04 | Smoke exposure device used for establishing chronic obstructive pulmonary disease model |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010193138XA CN101884569B (en) | 2010-06-04 | 2010-06-04 | Smoke exposure device used for establishing chronic obstructive pulmonary disease model |
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| Publication Number | Publication Date |
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| CN101884569A CN101884569A (en) | 2010-11-17 |
| CN101884569B true CN101884569B (en) | 2011-08-31 |
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| CN201010193138XA Expired - Fee Related CN101884569B (en) | 2010-06-04 | 2010-06-04 | Smoke exposure device used for establishing chronic obstructive pulmonary disease model |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102935018B (en) * | 2012-11-13 | 2014-11-26 | 天津开发区合普工贸有限公司 | Animal experiment device for passive smoke produced by human smoking |
| CN107006422A (en) * | 2017-03-30 | 2017-08-04 | 华南农业大学 | A kind of smoking causes the method for building up of tree shrew multiple organ fibrosis model |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091873A (en) * | 2007-04-21 | 2007-12-26 | 大连理工大学 | Technical method by using seawater to remove sulfur dioxide in smoke through membrane absorption method |
| CN101564288A (en) * | 2009-05-16 | 2009-10-28 | 大连大学 | Multifunctional device for simulating respiratory environment and inhalational molding and administrating medicament |
| CN201701329U (en) * | 2010-06-04 | 2011-01-12 | 浙江大学 | Smoke exposing device for building mold of chronic obstructive pulmonary disease |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003031946A1 (en) * | 2001-09-28 | 2003-04-17 | Japan Tobacco Inc. | Entire smoke exposure device |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091873A (en) * | 2007-04-21 | 2007-12-26 | 大连理工大学 | Technical method by using seawater to remove sulfur dioxide in smoke through membrane absorption method |
| CN101564288A (en) * | 2009-05-16 | 2009-10-28 | 大连大学 | Multifunctional device for simulating respiratory environment and inhalational molding and administrating medicament |
| CN201701329U (en) * | 2010-06-04 | 2011-01-12 | 浙江大学 | Smoke exposing device for building mold of chronic obstructive pulmonary disease |
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| CN101884569A (en) | 2010-11-17 |
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Granted publication date: 20110831 Termination date: 20130604 |