CN101879221B - Medical application of caulis polygoni multiflori - Google Patents
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Abstract
The invention discloses application of caulis polygoni multiflori aqueous extracts or extracting paste or amylase to the preparation of medicine for treating glucose tolerance lack, diabetes, adiposity, hyperuricemia or uarthritis. The invention also discloses the application of aqueous extracts or extracting paste prepared from caulis polygoni multiflori and sargentg loryvine according to a weight ratio of 1/1 to the preparation of medicine for treating glucose tolerance lack, diabetes, adiposity, hyperuricemia or uarthritis. The invention is obtained through sieving from 70 kinds of traditional Chinese medicine and 16 kinds of compositions which do not have the hyperglycemic, blood uric acid reduction, alpha-glucosaccharase inhabiting agents and xanthine oxidase inhabiting agents till now, and consists of the caulis polygoni multiflori or the caulis polygoni multiflori and the sargentg loryvine. The invention discovers the inhabiting effects on the alpha-glucosaccharase and the xanthine oxidase for the first time.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, be specifically related to the new medical applications of Caulis Polygoni Multiflori.
Background technology
Postprandial hyperglycemia is impaired glucose tolerance (IGT) Clinical symptoms; It also is the common symptom of 1 type and type 2 diabetes mellitus; With IGT, related [A Ceriello and S Colagiuri International Diabetes Federation guideline for management of postmeal glucose:a review of recommendations [J] the .Diabet Med of diabetic complication; 2008,25 (10): 151～11156.].In time control postprandial hyperglycemia, can make high-risk group's blood sugar recovery of 1/3 normal, reduce the generation that 1/3 high-risk group suffers from diabetes and its complication in the IGT stage.
In the 43rd EASD meeting in 2007, IDF issues " post-prandial glycemia administration guide ", classifies alpha-glucosidase inhibitor (AGIs) as a line medicine for this reason.Alpha-glucosidase inhibitor comprises acarbose and voglibose and miglitol.Such medicine can suppress to be positioned at the alpha-glucosidase of small intestinal near-end surface epithelial cell specifically; Delay hydrolysis to the carbohydrate of disaccharidase and multiple complicacy; Reversibility through to this enzyme suppresses, and intestinal is slowed down to the absorption of monosaccharide, controls postprandial hyperglycemia.
China IGT, diabetics have own distinct clinical characters, and its dietary structure is different from the westerner, is main with carbohydrate, and the blood glucose state of metabolic is different in the westerner, and the postprandial hyperglycemia ratio is high than the west.So it is the most suitable to select AGIs to intervene to be.Like IGT acarbose treatment group through following up a case by regular visits to 3.3 years; The danger of onset diabetes has descended 31%; Make the hypertensive sickness rate of new diagnosis descend 34% simultaneously; The danger of myocardial infarction morbidity descends 91%, and the sickness rate of any cardiovascular event reduces by 49% [Chiasson JL, Gomis R; Hanefeld M; Et al.The STOP-NIDDMTrial:an international study on the efficacy of an alpha-glucosidase inhibitor to prevent type 2 diabetes in a population with impaired glucose tolerance:rationale, design, and preliminary screening data:Study to Prevent Non-Insulin-Dependent Diabetes Mellitus [J] .Diabetes Care; 1998,21:1720～1725.].Acarbose is at Chinese type 2 diabetes mellitus patient blood sugar control effect remarkable [Pan, Chang-Yu; Landen; Harald.Post-Marketing Surveillance of Acarbose Treatment in Patients with Type 2 Diabetes Mellitus and Subj ects with Impaired Glucose Tolerance in China [J] .Clinical Drag Investigation; 2007,27 (6): 397～405.].Acarbose can also obviously descend its Body Mass Index (BMI) when reducing metabolism syndrome (MS) patient blood glucose value, and prompting patient's obese degree obviously improves; These effects maybe with the relevant (Ye Wenchun of its influence to the intestinal carbohydrate metabolism; Direction is bright, Liu Yun, etc. acarbose is to the intervention effect [J] of metabolism syndrome. Chinese Pharmaceutical; 2008,17 (13): 57-58.).
But above-mentioned this type inhibitor market price is all somewhat expensive, satisfy the low income patient for a long time with its postprandial hyperglycemia of intervening IGT, type 2 diabetes mellitus, and the depletion that delays the generation of type 2 diabetes mellitus or alleviate the beta Cell of islet function is the thing that part is not easy to adhere to.
Hyperuricemia is a kind of purine metabolism obstacle disease, and it is not only old people's height morbidity, but also the rejuvenation that becomes.Because urate crystal is deposited on beta Cell of islet, make its damage and to bring out 2 types urine sick, the incidence rate of its patient's diabetes about 5.1%～15.74%.Otherwise the long-term high sugared state infringement renal function of diabetics causes the glomerular filtration uric acid to descend; The tubular excretion ability goes down; Increased the weight of hyperuricemia again, the two reciprocal causation, [Li Changgui mutually promotes; Wang Bei. hyperuricemia and abnormal carbohydrate metabolism. Chinese medical information Leader, 2006.21 (22): 18.].Therefore, the control hyperuricemia is very crucial, and the choice drug of its treatment is an xanthine oxidase inhibitor; Mainly be the allopurinol of the listing sixties in last century, be used to suppress the process that purine base is converted into uric acid, its curative effect is leveraged to the present certainly always; But the side effect after using also clearly; Heating, allergic rash, stomachache, diarrhoea, leukocyte and thrombocytopenia can appear in the patient, even performance such as liver function injury, so the new xanthine oxidase inhibitor of research is very necessary.
China's natural resources of Chinese medicinal materials is abundant, and is cheap, the existing many reports of screening alpha-glucosidase inhibitor or xanthine oxidase inhibitor from single medicinal material or compound recipe.But seeing that the consideration of above-mentioned two aspect reasons single medicinal material or compositions, simultaneously as alpha-glucosidase and two kinds of inhibitor siftings of xanthine oxidase, does not appear in the newspapers as yet.Use Caulis Polygoni Multiflori, Caulis Polygoni Multiflori and Caulis Sargentodoxae compatible composition carry out the screening of these two kinds of enzyme inhibitor effects simultaneously, also do not appear in the newspapers.
Summary of the invention
Technical problem to be solved by this invention provides Caulis Polygoni Multiflori and the medical applications of its compositions aspect alpha-glucosidase and xanthine oxidase inhibitory action.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
The application in preparation treatment impaired glucose tolerance, diabetes, obesity, hyperuricemia or gout medicine of Caulis Polygoni Multiflori water extract or extractum or polysaccharide.
Wherein, Caulis Polygoni Multiflori water extract or extractum or polysaccharide suppress alpha-glucosidase and xanthine oxidase simultaneously.
Caulis Polygoni Multiflori and Caulis Sargentodoxae are by weight water extract or the extractum or the application of polysaccharide in preparation treatment impaired glucose tolerance, diabetes, obesity, hyperuricemia or gout medicine of 1: 1 compatibility.
Wherein, Caulis Polygoni Multiflori and Caulis Sargentodoxae suppress alpha-glucosidase and xanthine oxidase simultaneously by weight the water extract of 1: 1 compatibility or extractum or polysaccharide.
The method for preparing of the water extract of above-mentioned Caulis Polygoni Multiflori or Caulis Polygoni Multiflori and Caulis Sargentodoxae compatibility is following:
Get Caulis Polygoni Multiflori or Caulis Polygoni Multiflori and Caulis Sargentodoxae medical material, add the distilled water of 20 times of weight by weight 1: 1 compatibility, room temperature merceration 2h, water proof decocts 1h, filters, and adding distil water repeats to decoct 1 time again; Merge filtrating 2 times, be cooled to centrifugal 3000rpm/min after the room temperature, 10min concentrates on the supernatant part boiling water bath.
The extract making method of above-mentioned Caulis Polygoni Multiflori or Caulis Polygoni Multiflori and Caulis Sargentodoxae compatibility is following:
After preparing the water extract by the method for preparing of water extract, add concentration of alcohol and reach 50vt%, mixing, centrifugal 3000rpm/min after 4 ℃ of refrigerator overnight, 10min gets on the supernatant part boiling water bath and concentrates.
The method for preparing of the polysaccharide of above-mentioned Caulis Polygoni Multiflori or Caulis Polygoni Multiflori and Caulis Sargentodoxae compatibility is following:
By the precipitate with ethanol precipitate that extract making method obtains, add the dehydrated alcohol, acetone, ether each 2 times of 2 times of volumes successively, behind the mixing, through centrifugal 3000rpm/min, 10min dewaters successively, volatilizes to powder under the room temperature.
Beneficial effect: the present invention does not obtain from still having blood sugar lowering up to now, fall blood uric acid, screening 70 kinds of Chinese medicine of alpha-glucosidase inhibitor and xanthine oxidase inhibitor effect and the 16 kinds of compositionss; Form by Caulis Polygoni Multiflori or Caulis Polygoni Multiflori and Caulis Sargentodoxae, the inhibitory action of alpha-glucosidase and xanthine oxidase is first finds.
See that from theory of Chinese medical science Caulis Polygoni Multiflori is polygonum multiflorum thunb ratan Polygonum multiflorum Thunb among the we.Sweet in the mouth, little hardship, property is flat.GUIXIN, Liver Channel.Tranquilizing by nourishing the heart is arranged, dispel the wind, the effect of collateral dredging.In single medicinal material and prescription, all above-mentioned two kinds of enzymes there is inhibitory action, can also YIN and YANG balance regulating, the source of so being insomnia; Its network of passing through; Promoting blood circulation, nervous system and cardiovascular complication that " sugar poison " is caused, and the gout that hyperuricemia causes played the effect of controlling in advance.
Caulis Sargentodoxae is the stem of Lardizabalaceae plant Caulis Sargentodoxae Sargentodoxa cuneata (Oliv.) Rehd.et Wils..Bitter in the mouth, cool in nature, return liver, large intestine two warps.Effect is the eliminating carbuncle that relieves internal heat, promoting blood circulation to remove obstruction in the collateral, wind dispelling insecticide.Above-mentioned two kinds of enzyme inhibitions are all arranged in single medicinal material and prescription; Its QI and blood regulating; The pass through effect of collateral dredging; To reducing postprandial hyperglycemia, treatment impaired glucose tolerance, diabetes, obesity and internal organs channels be impaired to cause complication such as cardiovascular, and the gout that hyperuricemia causes is played an important role.
The specific embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that embodiment is described only to be used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Caulis Polygoni Multiflori and Caulis Sargentodoxae used in following examples are purchased in Jiangsu TCM Hospital, identify through professor Chen Jianwei of pharmaceutical college of Nanjing University of Traditional Chinese Medicine.
Embodiment 1: the preparation of Caulis Polygoni Multiflori water extract or extractum or polysaccharide.
The preparation of Caulis Polygoni Multiflori water extract: get Caulis Polygoni Multiflori 50g decoction pieces, add the distilled water of 20 times of weight, room temperature merceration 2h, water proof decocts 1h, filters, and adding distil water repeats to decoct 1 time again; Merge filtrating 2 times, be cooled to centrifugal 3000rpm/min after the room temperature, 10min concentrates on the supernatant part boiling water bath, and adding distil water is settled to 0.5g crude drug/mL.
Caulis Polygoni Multiflori extractum preparation: prepare the water extract by above-mentioned Caulis Polygoni Multiflori water extract method for preparing, add concentration of alcohol and reach 50vt%, mixing; Centrifugal 3000rpm/min after 4 ℃ of refrigerator overnight; 10min gets supernatant concentration, and adding distil water is settled to 0.5g crude drug/mL.
The preparation of Caulis Polygoni Multiflori polysaccharide:, add the dehydrated alcohol, acetone, ether each 2 times of 2 times of volumes successively, behind the mixing by the precipitate with ethanol precipitate that above-mentioned Caulis Polygoni Multiflori extractum method for preparing obtains; Through centrifugal 3000rpm/min, 10min dewaters successively, volatilizes to powder under the room temperature; Weigh; Its yield is 1.10%, and then, adding distil water is settled to 0.5g crude drug/mL.
Embodiment 2: the preparation of Caulis Sargentodoxae water extract or extractum or polysaccharide.
The preparation of Caulis Sargentodoxae water extract: get Caulis Sargentodoxae 50g decoction pieces, add the distilled water of 20 times of weight, room temperature merceration 2h, water proof decocts 1h, filters, and adding distil water repeats to decoct 1 time again; Merge filtrating 2 times, be cooled to centrifugal 3000rpm/min after the room temperature, 10min concentrates on the supernatant part boiling water bath, and adding distil water is settled to 0.5g crude drug/mL.
Caulis Sargentodoxae extractum preparation: prepare the water extract by above-mentioned Caulis Sargentodoxae water extract method for preparing, add concentration of alcohol and reach 50vt%, mixing; Centrifugal 3000rpm/min after 4 ℃ of refrigerator overnight; 10min gets supernatant concentration, and adding distil water is settled to 0.5g crude drug/mL.
The preparation of Caulis Sargentodoxae polysaccharide:, add the dehydrated alcohol, acetone, ether each 2 times of 2 times of volumes successively, behind the mixing by the precipitate with ethanol precipitate that above-mentioned Caulis Sargentodoxae extractum method for preparing obtains; Through centrifugal 3000rpm/min, 10min dewaters successively, volatilizes to powder under the room temperature; Weigh; Its yield is 0.90%, and then, adding distil water is settled to 0.5g crude drug/mL.
Embodiment 3: the water extract of Caulis Polygoni Multiflori and Caulis Sargentodoxae compatibility or the preparation of extractum or polysaccharide.
Caulis Polygoni Multiflori prepares with Caulis Sargentodoxae compatibility water extract: get Caulis Polygoni Multiflori 50g decoction pieces, Caulis Sargentodoxae 50g decoction pieces mixes, and adds the distilled water of 20 times of weight, and room temperature merceration 2h, water proof decocts 1h, filters, and adding distil water repeats to decoct 1 time again; Merge filtrating 2 times, be cooled to centrifugal 3000rpm/min after the room temperature, 10min concentrates on the supernatant part boiling water bath, and adding distil water is settled to 0.5g crude drug/mL.
Caulis Polygoni Multiflori and the preparation of Caulis Sargentodoxae compatibility extractum: prepare the water extract by above-mentioned Caulis Polygoni Multiflori and Caulis Sargentodoxae compatibility water extract method for preparing; Add concentration of alcohol and reach 50vt%; Mixing, centrifugal 3000rpm/min after 4 ℃ of refrigerator overnight, 10min; Get supernatant concentration, adding distil water is settled to 0.5g crude drug/mL.
The preparation of Caulis Polygoni Multiflori and Caulis Sargentodoxae compatibility polysaccharide:, add the dehydrated alcohol, acetone, ether each 2 times of 2 times of volumes successively, behind the mixing by the precipitate with ethanol precipitate that above-mentioned Caulis Polygoni Multiflori and Caulis Sargentodoxae compatibility extractum method for preparing obtain; Through centrifugal 3000rpm/min, 10min dewaters successively, volatilizes to powder under the room temperature; Weigh; Its yield is 1.15%, and then, adding distil water is settled to 0.5g crude drug/mL.
Embodiment 4: pharmacodynamic experiment.
Get water extract, extractum or the polysaccharide of embodiment 1～3 preparation and do following pharmacodynamic experiment.
One, alpha-glucosaccharase enzyme inhibitor research.
The experiment medicine:
Acarbose (composition: acarbose, every contains 50 milligrams, lot number: 113608), purchase in Bayer HealthCare Co; Doubly glad (composition: voglibose, every contains 0.2 milligram, lot number: 070403), military field, Tianjin medicine company limited product.
Experiment reagent:
Alpha-glucosidase derives from Saccharomyces cerevisia and purchases the company in Sigma; Paranitrophenol-α-pyranglucoside (pNPG) originates in German Merck company; Reduced glutathion, KHK import packing; Other reagent are homemade analytical pure.
Laboratory animal:
Male ICR mouse, the cleaning level, 18～20g purchases in Shanghai Slac Experimental Animal Co., Ltd. credit number: SCXK (Shanghai) 2007-0005.
Experiment I: the extract of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions suppresses alpha-glucosidase activity and measures.
Chinese medicine tube reaction system be the 67mmol/L kaliumphosphate buffer (37 ℃, pH6.8) 1.0mL, 3mmol/L glutathione solution 25 μ L; 64013nkat/L alpha-glucosaccharase enzymatic solution 25 μ L; Respectively with 100 μ L Caulis Polygoni Multiflori, the extract 1.0mg of Caulis Sargentodoxae and its compositions, 3.0mg, 5.0mg, the suitable crude drug/mL of 10.0mg, (positive drug acarbose concentration, dosage and Caulis Polygoni Multiflori, Caulis Sargentodoxae parallel) mixing with the water extract of its compositions; Behind 37 ℃ of water bath heat preservation 10min; Add 20mmol/L pNPG solution 15 μ L, put former water-bath relaying again and continue insurance behind the warm 10min, add 0.01mol/L Na
2CO
3After the solution 5mL cooling,, be determined at the absorbance (A) that discharges paranitrophenol (pNP) under the effect of enzyme in wavelength 400nm place with the zeroing of blank pipe.The alpha-glucosidase pipe adds alpha-glucosidase and does not add the pNPG in the extract that does not add Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions, blank pipe, supply volume by distilled water, and other reactions are the same.The Chinese medicine blank is managed its extract concentrations, dosage is parallel with middle pencil, and other operations are managed with blank.Alpha-glucosaccharase enzyme inhibition rate computing formula is following:
Enzymatic activity suppression ratio (I%)=[enzyme pipe A-(the blank pipe of middle pencil A-Chinese medicine A)]/enzyme pipe A * 100
The water extract of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to the influence of alpha-glucosidase activity, and the result sees table 1.
The water extract of table 1 Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to the influence of alpha-glucosidase activity
The extractum of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is seen table 2 to the result that influences of alpha-glucosidase activity.
The extractum of table 2 Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to the influence of alpha-glucosidase activity
Experiment II: the extract of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions suppresses ICR mice rat small intestine in vitro α-maltase activity and measures.
Behind the ICR mice fasting 24h, the dislocation cervical vertebra is put to death, and gets small intestinal upper end (about 10cm) immediately, with the cold saline flushing for several times; Shred, add the 0.1mol/LpH6.8 phosphate buffer of 5 times of 4 ℃ of pre-coolings, refiner 10000r/min in the ice bath, 1～2min; Repeatedly, until being homogenate, 4 ℃, 4000r/min; Centrifugal 20min gets the supernatant packing, and-20 ℃ freezing subsequent use.
Small intestinal homogenate α-maltase pipe adds distilled water 50 μ L; In pencil add the extract of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions of 50 μ L variable concentrations (it be respectively 0.025g, 0.05g, 0.10g, 0.15g, the suitable crude drug of 0.2g/mL), positive pencil acarbose, doubly glad (it is respectively 10mg/mL, 0.2mg/mL); Each pipe adds small intestinal homogenate supernatant 50 μ L then, and mixing is behind 37 ℃ of water bath heat preservation 10min; (the 1.16g maleic acid is dissolved among the 100mLlmol/L NaOH to add the maleic acid buffer that contains 42mmol/ L maltose more respectively; PH7.0) 50 μ L, behind the mixing, 37 ℃ of temperature are incubated 30min; Add Tris-HCl (6.1g Tris is dissolved among the 5mol/L HCl 8.5mL, transfers pH to 7.0 with NaOH) cessation reaction.Then, get each tube reaction liquid 20uL respectively, add enzyme phenol reagent 3mL, mixing behind 37 ℃ of water bath heat preservation 10min, with the zeroing of blank pipe, in wavelength 505nm place, is measured and is produced glucose content.Enzyme-added in the blank pipe not with maltose, supply volume with the distilled water of equivalent.The Chinese medicine blank is managed its extract concentrations, dosage is parallel with middle pencil, and other operations are managed with blank.Calculate rat small intestine in vitro α-maltase by following formula and suppress percentage rate.
Enzymatic activity suppression ratio (I%)=[enzyme pipe glucose A-(the blank pipe of middle pencil glucose A-Chinese medicine A)]/enzyme pipe glucose A) * 100
The water extract of table 3 Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to ICR mice rat small intestine in vitro α-maltase activity influence
The water extract of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions influences the result to ICR mice rat small intestine in vitro α-maltase activity and sees table 3.
The extractum of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions influences the result to ICR mice rat small intestine in vitro α-maltase activity and sees table 4.
The extractum of table 4 Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to ICR mice rat small intestine in vitro α-maltase activity influence
In addition, the 10mg/mL acarbose, the 0.2mg/mL voglibose is respectively 93.1% and 89.6% to ICR mice rat small intestine in vitro α-maltase suppression ratio.
Two, xanthine oxidase inhibitor research.
The experiment medicine:
(every contains 0.1 gram, lot number: 090804), purchase in Shanghai Xinyi Wanxiang Pharmaceutical Co., Ltd. to allopurinol.
Experiment reagent:
Hypoxanthine, xanthine oxidase are purchased the company in Sigma; Reagent such as oxammonium hydrochloride., p-anilinesulfonic acid., N-1-naphthalene ethylamine hydrochlorate, sodium tetraborate, EDTA-2 sodium, glacial acetic acid are homemade analytical pure.
Experiment III: the extract of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions suppresses xanthine oxidase activity and measures
Chinese medicine tube reaction system is 50 μ L pH8.2 buffer (20.8mmol/L potassium dihydrogen phosphates; 15.6mmol/L sodium tetraborate; O.1mmol/L EDTA-2 sodium) prepare O.1u/mL xanthine oxidase (XOD); Distilled water 750 μ L are respectively with the suitable crude drug/mL of extract 6.25mg, 12.5mg, 25mg, 50mg, 100mg of 100 μ L Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions.100 μ L positive drug allopurinol concentration 25ug, 50ug, 100ug, 150ug, 200ug/ml.Mixing behind 37 ℃ of water bath heat preservation 15min, adds 100 μ L 0.8mmol/L hypoxanthine-10mmol/L oxammonium hydrochloride. solution; Putting former water-bath relaying again continues insurance behind the warm 30min; Add 5.2mmol/L p-anilinesulfonic acid .-3.858mmol/L N-1-naphthalene ethylamine hydrochlorate solution 2.0mL, mixing is after the cooling; With the distilled water zeroing, measure absorbance (A) in wavelength 550nm place.The xanthine oxidase pipe adds xanthine oxidase and does not add the hypoxanthine in the extract that does not add Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions, blank pipe, supply volume by distilled water, and other reactions are the same.The Chinese medicine blank is managed its extract concentrations, dosage is parallel with middle pencil, and other operations are managed with blank.Xanthine oxidase suppression ratio computing formula is following:
Enzymatic activity suppression ratio (I%)=[(the blank pipe of enzyme pipe A-A)-(the blank pipe of middle pencil A-Chinese medicine A)]/(the blank pipe of enzyme pipe A-A) * 100
The water extract of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to the influence of xanthine oxidase activity, and the result sees table 5.
The water extract of table 5 Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to the influence of xanthine oxidase activity
The extractum of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is seen table 6 to the result that influences of xanthine oxidase activity.
The extractum of table 6 Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to the influence of xanthine oxidase activity
The polysaccharide of Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is seen table 7 to the result that influences of xanthine oxidase activity.
The polysaccharide of table 7 Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions is to the influence of xanthine oxidase activity
Allopurinol is seen table 8 to the result that influences of xanthine oxidase activity.
Table 8 allopurinol is to the influence of xanthine oxidase activity
According to our accumulation in this type of inhibitor research in the past, adopt relevant pure enzyme and the histaminase reported at present to unite screening, do reference with positive drug, the result is more reliable.
Pharmacodynamic analysis through Caulis Polygoni Multiflori, Caulis Sargentodoxae and its compositions; Caulis Polygoni Multiflori, Caulis Sargentodoxae single medicinal material water extract; Alpha-glucosidase and α-maltase inhibition effect are superior to outside other extract parts, and its compositions water extract and compositions extractum are substantially parallel to the inhibitory action of glycosidase; Caulis Polygoni Multiflori, Caulis Sargentodoxae single medicinal material water extract, the extractum of its compositions suppresses effect to xanthine oxidase and is superior to other extract parts.
The extractum for preparing in the experiment is polarity, does not influence enzymatic reaction system, and can avoid the nonpolar of direct ethanol extract, needs to add the cosolvent dissolving, and causes the false-positive phenomenon of inhibition result.
In a word, above-mentioned Chinese medicine and its compositions are based on suppressing alpha-glucosidase (pure enzyme and little intestinal maltase) and two kinds of enzyme effects of xanthine oxidase, and be consistent with the effect of acarbose and allopurinol; Thereby think to have the post-prandial glycemia of falling, the blood uric acid dual-use function falls, and its aboundresources of this product; Cheap; Safety non-toxic is so can be used for treating impaired glucose tolerance, diabetes, obesity, diseases such as hyperuricemia, gout.
Claims (4)
1. Caulis Polygoni Multiflori water extract or extractum or the polysaccharide application in preparation treatment impaired glucose tolerance, diabetes, hyperuricemia or gout medicine;
Wherein, described Caulis Polygoni Multiflori water extract prepares as follows: Caulis Polygoni Multiflori, add the distilled water of 20 times of weight, and room temperature merceration 2h, water proof decocts 1h, filters, and adding distil water repeats to decoct 1 time again; Merge filtrating 2 times, be cooled to centrifugal 3000rpm/min after the room temperature, 10min concentrates on the supernatant part boiling water bath;
Wherein, Described Caulis Polygoni Multiflori extractum prepares as follows: after preparing Caulis Polygoni Multiflori water extract by the method for preparing of said Caulis Polygoni Multiflori water extract; Add concentration of alcohol and reach 50vt%, mixing, centrifugal 3000rpm/min after 4 ℃ of refrigerator overnight; 10min gets on the supernatant part boiling water bath concentrated;
Wherein, Described Caulis Polygoni Multiflori polysaccharide prepares as follows: the precipitate with ethanol precipitate that obtains by said Caulis Polygoni Multiflori extract making method; The dehydrated alcohol, acetone, ether each 2 times that add 2 times of volumes successively are behind the mixing, through centrifugal 3000rpm/min; 10min dewaters successively, volatilizes to powder under the room temperature.
2. application according to claim 1 is characterized in that Caulis Polygoni Multiflori water extract or extractum or polysaccharide suppress alpha-glucosidase and xanthine oxidase simultaneously.
3. Caulis Polygoni Multiflori and Caulis Sargentodoxae are by weight water extract or the extractum or the application of polysaccharide in preparation treatment impaired glucose tolerance, diabetes, obesity, hyperuricemia or gout medicine of 1: 1 compatibility;
Wherein, described Caulis Polygoni Multiflori and Caulis Sargentodoxae prepare by weight the water extract of 1: 1 compatibility as follows: Caulis Polygoni Multiflori and Caulis Sargentodoxae add the distilled water of 20 times of weight by weight the medical material of 1: 1 compatibility; Room temperature merceration 2h; Water proof decocts 1h, filters, and adding distil water repeats to decoct 1 time again; Merge filtrating 2 times, be cooled to centrifugal 3000rpm/min after the room temperature, 10min concentrates on the supernatant part boiling water bath;
Wherein, Described Caulis Polygoni Multiflori and Caulis Sargentodoxae prepare by weight the extractum of 1: 1 compatibility as follows: prepare Caulis Polygoni Multiflori and Caulis Sargentodoxae by said Caulis Polygoni Multiflori and Caulis Sargentodoxae behind the water extract of 1: 1 compatibility by weight the method for preparing of the water extract of 1: 1 compatibility; Add concentration of alcohol and reach 50vt%, mixing, centrifugal 3000rpm/min after 4 ℃ of refrigerator overnight; 10min gets on the supernatant part boiling water bath concentrated;
Wherein, Described Caulis Polygoni Multiflori and Caulis Sargentodoxae prepare by weight the polysaccharide of 1: 1 compatibility as follows: the precipitate with ethanol precipitate that obtains by weight the extract making method of 1: 1 compatibility by said Caulis Polygoni Multiflori and Caulis Sargentodoxae; The dehydrated alcohol, acetone, ether each 2 times that add 2 times of volumes successively are behind the mixing, through centrifugal 3000rpm/min; 10min dewaters successively, volatilizes to powder under the room temperature.
4. application according to claim 3 is characterized in that Caulis Polygoni Multiflori and Caulis Sargentodoxae suppress alpha-glucosidase and xanthine oxidase simultaneously by weight the water extract of 1: 1 compatibility or extractum or polysaccharide.
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| CN101879221B true CN101879221B (en) | 2012-10-24 |
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| CN102697822B (en) * | 2012-06-04 | 2014-04-09 | 江苏大学 | Application of sargentg loryvine stem active fraction to preparation of anti-inflammatory-immune and anti-ageing medicaments |
| CN102754825B (en) * | 2012-06-24 | 2013-10-30 | 宁波市鄞州福瑞达生物科技有限公司 | Health food for preventing gout |
| CN103393761A (en) * | 2013-08-02 | 2013-11-20 | 中华全国供销合作总社南京野生植物综合利用研究院 | Application of nymphaea hybrid extract in preparing drugs for treatment of gout, hyperuricemia and uric acid nephropathy and preparation method of nymphaea hybrid extract |
| CN106860641A (en) * | 2017-02-22 | 2017-06-20 | 山西医科大学 | A kind of Chinese medicine composition for effecting a radical cure urarthritis pain |
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| Title |
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| 旷惠桃.中医药治疗痛风研究概述.《湖南中医杂志》.2005,第21卷(第02期),79-81. * |
| 谢幼红.五藤五皮饮治疗痛风29例临床分析.《北京中医》.1998,(第02期),32-33. * |
| 谢纬等.中医药治疗痛风的进展.《河南中医学院学报》.2003,第16卷(第03期),86-88. * |
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