CN101875975A - Triple PCR-RFLP method for identifying mutton and duck meat - Google Patents
Triple PCR-RFLP method for identifying mutton and duck meat Download PDFInfo
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- CN101875975A CN101875975A CN 201010226683 CN201010226683A CN101875975A CN 101875975 A CN101875975 A CN 101875975A CN 201010226683 CN201010226683 CN 201010226683 CN 201010226683 A CN201010226683 A CN 201010226683A CN 101875975 A CN101875975 A CN 101875975A
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Abstract
The invention relates to a PCR-RFLP method for identifying mutton and duck meat, which belongs to the field of molecular biological detections. The invention designs a method for identifying the meat species source of a mutton product, and the method overcomes the defects of low accuracy of the current method for identifying the mutton and the duck meat, and provides a PCR-RFLP detection technique for identifying the mutton and the duck meat. The method for identifying the mutton and the duck meat by the following steps of: extracting the DNA of a sample; adopting a universal primer for amplification and gel electrophoresis for detection; and using Bsu36I and SpeI restriction endonuclease to perform PCR-RFLP analysis respectively to ensure that the mutton and duck meat are identified. The PCR-RFLP method has the characteristics of simplicity, quickness, low cost and high accuracy.
Description
Technical field
The invention belongs to the molecular Biological Detection field, relate to the discriminating of mutton and duck.
Technical background
Contain abundant quality protein and a large amount of mineral element etc. in the mutton, have very high nutritive value.Eating mutton severe winter can beneficial gas qi-restoratives, stimulates circulation, and strengthens the resisting cold ability.The traditional Chinese medical science thinks that mutton also has the effect of kidney invigorating and YANG supporting, so mutton is subjected to people's favor deeply.Yet in recent years, some illegal retailers sell the incident of cheating the human consumer with the duck string as mutton cubes roasted on a skewer and occur repeatedly, this behavior of selling with the alternative mutton of duck not only brings loss to the human consumer, causes detrimentally affect also for the sound development of mutton industry.In order to ensure consumers in general's rights and interests,, need differentiate accurately mutton and duck for the law enfrocement official provides technical support.
When fresh meat was carried out the sense organ discriminating, according to the characteristics difference of some species meat at aspects such as color, smell, flavour, fiber thickness, by " see, hear, touch " means, comprehensive three judgements that main points draw were carried out sense organ to meat and are differentiated.There are the following problems in actual applications for this: for the meat product through processing, owing to add materials such as pigment, foodstuff additive, spices, be difficult to accurately differentiate by the sense organ means.For example: the counterfeiter brushes sheep oil with the duck string, adds materials such as seasoning matter, is difficult to check by traditional sense organ method.
Immunology detection is based on the immune response of soluble antigen and antibody, uses the method that interspecies specific antigen carries out design studies.The ELISA method that GiovannacciI etc. detected animal species in 2005 has been carried out comprehensive review, thinks that sample can influence the detection accuracy of ELISA method through the heat treated of differing temps.Particularly after sample process pyroprocessing, sex change has taken place in albumen, has changed the specific antigen determinant, therefore is difficult to differentiate and distinguish animal species.The test kit sensitivity that is used for animal derived detection at present is lower, is unsuitable for promoting the use of.
The PCR-RFLP technology is quick, easy, existing relevant report aspect the kind discriminating of meat product, but do not see the report that mutton and duck discriminating aspect are arranged.
Summary of the invention
The objective of the invention is to solve present mutton and duck discrimination method defects of low accuracy, the PCR-RFLP method that provides a kind of mutton and duck to differentiate replaces the malpractice of mutton sale that technical support is provided for hitting duck.
Mutton and duck can be differentiated by the following method: one, will get the EP pipe (the EP pipe is answered autoclaving) that 50mg sample to be checked is put into 1.5mL, and it be shredded with little scissors.Two, the SDS solution 15 μ L that add STE extract 600 μ L, ProK (20mg/mL) 20 μ L and 20%, abundant mixing on the earthquake device.Three, the EP pipe is inserted water-bath, 55 ℃ of digestion electrophorograms." the moon " negative control sample among three figure, M are mark in the molecular weight.
Embodiment
Present embodiment one: one, will get the EP pipe (the EP pipe is answered autoclaving) that 50mg sample to be checked is put into 1.5mL, it be shredded with little scissors.Two, the SDS solution 15 μ L that add STE extract 600 μ L, ProK (20mg/mL) 20 μ L and 20%, abundant mixing on the earthquake device.Three, the EP pipe is inserted water-bath, 55 ℃ of digestion are spent the night (12-16h), can take out during this period and put upside down mixing several times, so that digestion thoroughly.Four, the EP pipe is taken out, every pipe adds the saturated phenol of Tris of 600 μ L, shakes mixing 15min gently, then at the centrifugal 10min of 12000rpm.Five, shift supernatant liquor to new EP pipe, add isopyknic phenol chloroform isoamyl alcohol mixed solution (phenol: chloroform: the primary isoamyl alcohol volume ratio is 25: 24: 1), put upside down mixing 10min, then the centrifugal 10min of 12000rpm.Six, shift supernatant liquor to new EP pipe, add isopyknic chloroform isoamyl alcohol mixed solution (chloroform: the primary isoamyl alcohol volume ratio is 24: 1), mixing 10min gently is then at the centrifugal 10min of 12000rpm.Seven, shift supernatant liquor to new EP pipe, add 1mL ice ethanol earlier, add the NaAc (3M) of 60 μ L again, level is shaken gently, and as seen cotton-shaped, white DNA occurs.The EP pipe is put into-20 ℃ of freezing 30min of refrigerator, and then at the centrifugal 10min of 12000rpm, visible DNA is sunken to the pipe end.Abandon dehydrated alcohol, use 75% washing with alcohol DNA twice again, then at the centrifugal 10min of 12000rpm; After the seasoning, add the dissolving of 50 μ L TE solution.Eight, pcr amplification and detection: primer general upstream primer the F:5 '-TAC CAT GAG GAC AAA TAT CAT TCT G-3 ' and general downstream primer the R:5 '-CCT CCT AGT TTG TTA GGG ATT GAT CG-3 ' of all vertebrates Cytb sequences that can increase.PCR reaction cumulative volume is 25 μ L, 10 * PCR Buffer, 2.5 μ L wherein, Taq archaeal dna polymerase 0.2 μ L (2.5U/ μ L), dNTPs (2.5mmol/L) 2 μ L, each 2 μ L of the upstream and downstream primer of 10pmol/ μ L, template DNA 250ng, sterile purified water is supplied cumulative volume.Amplification condition is: 95 ℃ of sex change 10min, and 95 ℃ of sex change 45s, 53 ℃ of annealing 60s, 72 ℃ are extended 60s, continue to extend 7min at 72 ℃ after 35 circulations.The PCR product of getting 4 μ L mixes with 6 * loadding buffer of 1 μ L, adopts the TAE buffering system, and 2% sepharose (adding staining agent Gold view I) about 25min under 5v/cm constant voltage deposition condition observes and record in gel imaging system.Nine, Bsu36I restriction analysis: the PCR product in the step 8 is carried out PCR-RFLP with restriction enzyme Bsu36I analyze; Bsu36I can only be cut into the PCR product of duck the fragment of 95bp and 377bp, and can not cut the PCR product of goat and sheep, and the bar that whether can detect 95bp and 377bp according to the sepharose detected result brings and judges whether to be duck.The enzyme system of cutting is 20 μ L, wherein: DNA (PCR product) 1 μ g, 10 * NEB buffer, 2 μ L, 100 * BSA0.2 μ L, Bsu36I restriction enzyme (10000U/mL) 0.5 μ L, sterile purified water is supplied volume.Mixing and centrifugal back are at 37 ℃ of digestion 16h, handle 20min deactivation endonuclease activity at 65 ℃ then, getting 7 μ LPCR products mixes with 1 μ L, 6 * loadding buffer, adopt the TAE buffering system, 2% sepharose (adding Gold view I staining agent) about 25min under 5v/cm constant voltage deposition condition observes and record in gel imaging system.Ten, SpeI restriction analysis: the PCR product in the step 8 is carried out PCR-RFLP with restriction enzyme SpeI analyze; SpeI can be cut into the PCR product of sheep and goat 326 and spend the night (12-16h), can take out during this period and put upside down mixing several times, so that digestion thoroughly.Four, the EP pipe is taken out, every pipe adds the saturated phenol of Tris of 600 μ L, shakes mixing 15min gently, then at the centrifugal 10min of 12000rpm.Five, shift supernatant liquor to new EP pipe, add isopyknic phenol chloroform isoamyl alcohol mixed solution (phenol: chloroform: the primary isoamyl alcohol volume ratio is 25: 24: 1), put upside down mixing 10min, then the centrifugal 10min of 12000rpm.Six, shift supernatant liquor to new EP pipe, add isopyknic chloroform isoamyl alcohol mixed solution (chloroform: the primary isoamyl alcohol volume ratio is 24: 1), mixing 10min gently is then at the centrifugal 10min of 12000rpm.Seven, shift supernatant liquor to new EP pipe, add 1mL ice ethanol earlier, add the NaAc (3M) of 60 μ L again, level is shaken gently, and as seen cotton-shaped, white DNA occurs.The EP pipe is put into-20 ℃ of freezing 30min of refrigerator, and then at the centrifugal 10min of 12000rpm, visible DNA is sunken to the pipe end.Abandon dehydrated alcohol, use 75% washing with alcohol DNA twice again, then at the centrifugal 10min of 12000rpm; After the seasoning, add the dissolving of 50 μ L TE solution.Eight, pcr amplification and detection: primer general upstream primer the F:5 '-TAC CAT GAG GAC AAA TAT CATTCT G-3 ' and general downstream primer the R:5 '-CCT CCT AGT TTG TTA GGG ATT GAT CG-3 ' of all vertebrates Cyt b sequences that can increase.PCR reaction cumulative volume is 25 μ L, 10 * PCR Buffer, 2.5 μ L wherein, Taq archaeal dna polymerase 0.2 μ L (2.5U/ μ L), dNTPs (2.5mmol/L) 2 μ L, each 2 μ L of the upstream and downstream primer of 10pmol/ μ L, template DNA 250ng, sterile purified water is supplied cumulative volume.Amplification condition is: 95 ℃ of sex change 10min, and 95 ℃ of sex change 45s, 53 ℃ of annealing 60s, 72 ℃ are extended 60s, continue to extend 7min at 72 ℃ after 35 circulations.The PCR product of getting 4 μ L mixes with 6 * loadding buffer of 1 μ L, adopts the TAE buffering system, and 2% sepharose (adding staining agent Gold view I) about 25min under 5v/cm constant voltage deposition condition observes and record in gel imaging system.Nine, Bsu36I restriction analysis: the PCR product in the step 8 is carried out PCR-RFLP with restriction enzyme Bsu36I analyze; Bsu36I can only be cut into the PCR product of duck the fragment of 95bp and 377bp, and can not cut the PCR product of goat and sheep, and the bar that whether can detect 95bp and 377bp according to the sepharose detected result brings and judges whether to be duck.Ten, SpeI restriction analysis: the PCR product in the step 8 is carried out PCR-RFLP with restriction enzyme SpeI analyze; SpeI can be cut into 326bp and 146bp with the PCR product of sheep and goat, but can not cut the PCR product of duck, and the bar that whether can detect 326bp and 146bp according to the sepharose detected result brings and judges whether to be mutton.
The present invention adopts the PCR-RFLP method in discrimination process, have easy, quick, cheapness and the high characteristics of accuracy.
Description of drawings
Fig. 1 is the gel electrophoresis figure that the sheep that step 8 obtains in the embodiment one (S), goat (G) and duck (D) PCR product detect; Fig. 2 is that the PCR product of step 9 obtains step 8 in the embodiment one with Bsu36I restriction endonuclease digestion in the embodiment one sheep (S), goat (G) and duck (D) carries out the gel electrophoresis figure that PCR-RFLP analyzes the back detection; Fig. 3 is that the PCR product of the sheep that step 10 obtains step 8 in the embodiment one with the SpeI digestion with restriction enzyme in the embodiment one (S), goat (G) and duck (D) carries out gel bp and the 146bp that PCR-RFLP analyzes the back detection, but can not cut the PCR product of duck, the bar that whether can detect 326bp and 146bp according to the sepharose detected result brings and judges whether to be mutton.The enzyme system of cutting is 20 μ L, wherein: DNA (PCR product) 1 μ g, 10 * NEBbuffer, 2 μ L, 100 * BSA, 0.2 μ L, SpeI restriction enzyme (10000U/mL) 0.5 μ L, sterile purified water is supplied volume.Mixing and centrifugal back are at 37 ℃ of digestion 16h, handle 20min deactivation endonuclease activity at 65 ℃ then, getting 7 μ L PCR products mixes with 1 μ L, 6 * loadding buffer, adopt the TAE buffering system, 2% sepharose (adding Gold view I staining agent) about 25min under 5v/cm constant voltage deposition condition observes and record in gel imaging system.
Attached: the restriction enzyme site of differentiating sheep, goat and the used restriction enzyme of duck
Restriction enzyme Bsu36I (CC ↓ TNAGG)
Restriction enzyme SpeI (A ↓ CTAGT)
Attached: solution preparation table
STE extract: 10mL Tris-HCl (1M; PH 8.0), 2mL EDTA (0.5M; PH 8.0), 100mL NaAc (1M), 888mL water, autoclaving.
20%SDS:200g SDS is dissolved in the 900mL water, with hydrochloric acid adjust pH to 7.2, adds water and is settled to 1000mL.
1M Tris-HCl:121.4g Tris is dissolved in the 800mL distilled water, with hydrochloric acid adjust pH to 8.0, is settled to 1000mL, autoclaving.
TE damping fluid (pH 8.0): 1mL Tris-HCl (1M; PH 8.0), 0.1mL EDTA (0.5M; PH 8.0), add distilled water and be settled to 50mL, autoclaving.
20mg/mL ProK:100mg Proteinase K is dissolved in 5mL sterilization distilled water, and-20 ℃ of packing are preserved.
TE damping fluid (pH 8.0): 1mL Tris-HCl (1M; PH 8.0), 0.1mL EDTA (0.5M; PH 8.0), add distilled water and be settled to 50mL, autoclaving.
Claims (5)
1. a PCR-RFLP method of differentiating mutton and duck is characterized in that and mutton and duck can be differentiated by the following method: one, will get the EP pipe (the EP pipe is answered autoclaving) that 50mg sample to be checked is put into 1.5mL, and with little scissors it be shredded.Two, the SDS solution 15 μ L that add STE extract 600 μ L, ProK (20mg/mL) 20 μ L and 20%, abundant mixing on the earthquake device.Three, the EP pipe is inserted water-bath, 55 ℃ of digestion are spent the night (12-16h), can take out during this period and put upside down mixing several times, so that digestion thoroughly.Four, the EP pipe is taken out, every pipe adds the saturated phenol of Tris of 600 μ L, shakes mixing 15min gently, then at the centrifugal 10min of 12000rpm.Five, shift supernatant liquor to new EP pipe, add isopyknic phenol chloroform isoamyl alcohol mixed solution (phenol: chloroform: the primary isoamyl alcohol volume ratio is 25: 24: 1), put upside down mixing 10min, then the centrifugal 10min of 12000rpm.Six, shift supernatant liquor to new EP pipe, add isopyknic chloroform isoamyl alcohol mixed solution (chloroform: the primary isoamyl alcohol volume ratio is 24: 1), mixing 10min gently is then at the centrifugal 10min of 12000rpm.Seven, shift supernatant liquor to new EP pipe, add 1mL ice ethanol earlier, add the NaAc (3M) of 60 μ L again, level is shaken gently, and as seen cotton-shaped, white DNA occurs.The EP pipe is put into-20 ℃ of freezing 30min of refrigerator, and then at the centrifugal 10min of 12000rpm, visible DNA is sunken to the pipe end.Abandon dehydrated alcohol, use 75% washing with alcohol DNA twice again, then at the centrifugal 10min of 12000rpm; After the seasoning, add the dissolving of 50 μ L TE solution.Eight, pcr amplification and detection: primer general upstream primer the F:5 '-TAC CAT GAG GAC AAA TAT CAT TCT G-3 ' and general downstream primer the R:5 '-CCT CCT AGT TTG TTA GGG ATT GAT CG-3 ' of all vertebrates Cyt b sequences that can increase.PCR reaction cumulative volume is 25 μ L, 10 * PCR Buffer, 2.5 μ L wherein, Taq archaeal dna polymerase 0.2 μ L (2.5U/ μ L), dNTPs (2.5mmol/L) 2 μ L, each 2 μ L of the upstream and downstream primer of 10pmol/ μ L, template DNA 250ng, sterile purified water is supplied cumulative volume.Amplification condition is: 95 ℃ of sex change 10min, and 95 ℃ of sex change 45s, 53 ℃ of annealing 60s, 72 ℃ are extended 60s, continue to extend 7min at 72 ℃ after 35 circulations.The PCR product of getting 4 μ L mixes with 6 * loadding buffer of 1 μ L, adopts the TAE buffering system, and 2% sepharose (adding staining agent Gold view I) about 25min under 5v/cm constant voltage deposition condition observes and record in gel imaging system.Nine, Bsu36I restriction analysis: the PCR product in the step 8 is carried out PCR-RFLP with restriction enzyme Bsu36I analyze; Bsu36I can only be cut into the PCR product of duck the fragment of 95bp and 377bp, and can not cut the PCR product of goat and sheep, and the bar that whether can detect 95bp and 377bp according to the sepharose detected result brings and judges whether to be duck.Ten, SpeI restriction analysis: the PCR product in the step 8 is carried out PCR-RFLP with restriction enzyme SpeI analyze; SpeI can be cut into 326bp and 146bp with the PCR product of sheep and goat, but can not cut the PCR product of duck, and the bar that whether can detect 326bp and 146bp according to the sepharose detected result brings and judges whether to be mutton.
2. a kind of PCR-RFLP method of differentiating mutton and duck according to claim 1 is characterized in that step 2 STE extract.
3. a kind of PCR-RFLP method of differentiating mutton and duck according to claim 1 is characterized in that amplimer is with general upstream primer F:5 '-TAC CAT GAG GAC AAA TAT CAT TCT G-3 ' and general downstream primer R:5 '-CCT CCT AGT TTG TTA GGG ATT GAT CG-3 ' in the step 8.
4. a kind of PCR-RFLP method of differentiating mutton and duck according to claim 1 is characterized in that step 9 carries out PCR-RFLP with restriction enzyme Bsu36I and analyze.
5. a kind of PCR-RFLP method of differentiating mutton and duck according to claim 1 is characterized in that step 10 carries out PCR-RFLP with restriction restriction endonuclease SpesI and analyze.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103290131A (en) * | 2013-06-18 | 2013-09-11 | 徐鹏 | Primer pair and kit for distinguishing Channa argus and Channa maculata, and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) detection method |
| CN104232742A (en) * | 2013-06-08 | 2014-12-24 | 苏州市产品质量监督检验所 | Method for detecting duck-derived component in food through fluorescent real-time PCR method |
| CN106048034A (en) * | 2016-07-01 | 2016-10-26 | 北京市食品安全监控和风险评估中心 | Method for scanning and analyzing animal-source components in food based on PCR-RLFP-DHPLC technology |
| US10986817B2 (en) | 2014-09-05 | 2021-04-27 | Intervet Inc. | Method and system for tracking health in animal populations |
| US10986816B2 (en) | 2014-03-26 | 2021-04-27 | Scr Engineers Ltd. | Livestock location system |
| US11071279B2 (en) | 2014-09-05 | 2021-07-27 | Intervet Inc. | Method and system for tracking health in animal populations |
| USD990062S1 (en) | 2020-06-18 | 2023-06-20 | S.C.R. (Engineers) Limited | Animal ear tag |
| USD990063S1 (en) | 2020-06-18 | 2023-06-20 | S.C.R. (Engineers) Limited | Animal ear tag |
| US11832587B2 (en) | 2020-06-18 | 2023-12-05 | S.C.R. (Engineers) Limited | Animal tag |
| US11832584B2 (en) | 2018-04-22 | 2023-12-05 | Vence, Corp. | Livestock management system and method |
| US11864529B2 (en) | 2018-10-10 | 2024-01-09 | S.C.R. (Engineers) Limited | Livestock dry off method and device |
| US11960957B2 (en) | 2020-11-25 | 2024-04-16 | Identigen Limited | System and method for tracing members of an animal population |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998054360A1 (en) * | 1997-05-30 | 1998-12-03 | Pig Improvement Company Uk Limited | Methods for analyzing animal products |
| CN101712996A (en) * | 2009-12-14 | 2010-05-26 | 中国科学院昆明动物研究所 | Method for quickly identifying categories of meat and dried meat products of five domestic animals |
-
2010
- 2010-07-15 CN CN 201010226683 patent/CN101875975A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998054360A1 (en) * | 1997-05-30 | 1998-12-03 | Pig Improvement Company Uk Limited | Methods for analyzing animal products |
| CN101712996A (en) * | 2009-12-14 | 2010-05-26 | 中国科学院昆明动物研究所 | Method for quickly identifying categories of meat and dried meat products of five domestic animals |
Non-Patent Citations (2)
| Title |
|---|
| 《中国优秀硕士学位论文全文数据库农业科技辑》 20110215 冯海永 羊肉产品肉种来源的分子追溯研究 第28-32页 1-5 , 第2期 2 * |
| 《南京农业大学学报》 20081231 高琳等 应用PCR-RFLP法鉴别肉制品中的猪和牛源性成分 第135-138页 1-5 第31卷, 第2期 2 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232742A (en) * | 2013-06-08 | 2014-12-24 | 苏州市产品质量监督检验所 | Method for detecting duck-derived component in food through fluorescent real-time PCR method |
| CN103290131A (en) * | 2013-06-18 | 2013-09-11 | 徐鹏 | Primer pair and kit for distinguishing Channa argus and Channa maculata, and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) detection method |
| US10986816B2 (en) | 2014-03-26 | 2021-04-27 | Scr Engineers Ltd. | Livestock location system |
| US11963515B2 (en) | 2014-03-26 | 2024-04-23 | S.C.R. (Engineers) Limited | Livestock location system |
| US10986817B2 (en) | 2014-09-05 | 2021-04-27 | Intervet Inc. | Method and system for tracking health in animal populations |
| US11071279B2 (en) | 2014-09-05 | 2021-07-27 | Intervet Inc. | Method and system for tracking health in animal populations |
| CN106048034A (en) * | 2016-07-01 | 2016-10-26 | 北京市食品安全监控和风险评估中心 | Method for scanning and analyzing animal-source components in food based on PCR-RLFP-DHPLC technology |
| US11832584B2 (en) | 2018-04-22 | 2023-12-05 | Vence, Corp. | Livestock management system and method |
| US11864529B2 (en) | 2018-10-10 | 2024-01-09 | S.C.R. (Engineers) Limited | Livestock dry off method and device |
| US11832587B2 (en) | 2020-06-18 | 2023-12-05 | S.C.R. (Engineers) Limited | Animal tag |
| USD990063S1 (en) | 2020-06-18 | 2023-06-20 | S.C.R. (Engineers) Limited | Animal ear tag |
| USD990062S1 (en) | 2020-06-18 | 2023-06-20 | S.C.R. (Engineers) Limited | Animal ear tag |
| US11960957B2 (en) | 2020-11-25 | 2024-04-16 | Identigen Limited | System and method for tracing members of an animal population |
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Application publication date: 20101103 |