CN101869219A - Preparation method of siberian tiger intestinal tract probiotics - Google Patents
Preparation method of siberian tiger intestinal tract probiotics Download PDFInfo
- Publication number
- CN101869219A CN101869219A CN 201010194464 CN201010194464A CN101869219A CN 101869219 A CN101869219 A CN 101869219A CN 201010194464 CN201010194464 CN 201010194464 CN 201010194464 A CN201010194464 A CN 201010194464A CN 101869219 A CN101869219 A CN 101869219A
- Authority
- CN
- China
- Prior art keywords
- probiotics
- grams
- intestinal tract
- bacterium
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a preparation method of siberian tiger intestinal tract probiotics, which comprises the following steps that: first preparing the fermentation broth of five strains of probiotics, i.e. lactobacillus acidophilus, lactobacillus plantarum, lactococcus lactis, lactobacillus rhamnosus and bifidobacterium bifidum, removing supernatant at 5000r/min, respectively adopting 10g of mud of each type of probiotic, adding 4g of agaricus mushroom powder, mixing with 40g of mixed colloid containing 2.5 percent sodium alginate and 0.5 percent flaxseed gum, adding edible oil with 1.5 times of volume and 1.5 percent CaCl2 solution to be calcified for 3h, washing a gel ball for three times with saline water, putting the gel ball into 0.6 percent chitosan solution with the pH value of 5.5 to carry out film reaction for 15min and be taken out, flushing with the sterilizing saline water and putting into 55mM Citric acid solution to be liquefied for 30min to obtain the probiotic mud, finally cooling and drying to obtain intestinal tract probiotics powder, and obtaining the intestinal tract probiotics through the compounding of various probiotic powder.
Description
(1) technical field
The present invention relates to a kind of preparation method of siberian tiger intestinal tract probiotics, belong to bioengineering field.
(2) background technology
In recent decades; because mankind's activity causes the habitat fragmentation and is short of food environment; and human unlawful hunt, slaughter with trade and cause wild northeastern tiger non-hibernating eggs group quantity sharply to descend, northeastern tiger has been put into Chinese I level and has watched for animals and be put into the endangered species of wild fauna and flora kind.There is family more than 120 wherein to have family more than 40 that northeastern tiger is arranged approximately at present in the various types of zoos of China.Especially be that the expansion of northeastern tiger animal population makes a great contribution with the big cat breeding base of representatives such as the male gloomy Xiong Hushanzhuan of Heilungkiang northeastern tiger wooden land, Guilin, existing stable breeding Captive Population will become wild stocks and enlarge the important reserve force that develops.
But along with the increase of Captive Population quantity and the increasing of stocking density, the serious pathogenetic chance of infection of some harm also increases thereupon.Especially northeastern tiger young baby, resistance is relatively poor, and disease of digestive tracts such as Escherichia coli, clostridieum welchii, parvovirus infections often take place, and has greatly reduced the survival rate of young tiger.We think that under the normal condition, generally there is normal flora in the children in the northeastern tiger enteron aisle age in research.Keep relative equilibrium by mutual restriction between the flora, the bacterium in the normal flora is generally not pathogenic to tiger, and on the contrary, some bacterium has the physiological action that benefit is given birth to brave body.For example the Bifidobacterium bacterial metabolism produces multivitamin, can promote growing of brave body.Yet, owing to the resistance of children tiger is relatively poor, when various emergency reactions and a large amount of use antibiotic therapy disease, all very easily cause the normal flora imbalance in the enteron aisle, cause the generation of some infectiouss disease of the digestive tract.After having used probiotics, progressively recovered normal flora in the enteron aisle, improved gastrointestinal function, improved the food digestion rate, suppressed the growth of enteron aisle spoilage organisms, strengthened immunity of organisms.
It is starting strain that the present invention adopts 5 strain beneficial bacteria of intestinal tract, make microecological microbial agent through after the external embedding, make an addition to northeastern tiger cub in the feed, the feedback that comes 1 year shows more, the brave disease of digestive tract of back children of feeding takes place obviously to reduce, and the brave survival rate of children obviously improves.
(3) summary of the invention
The object of the invention is to provide a kind of preparation method of siberian tiger intestinal tract probiotics.
The object of the present invention is achieved like this: related percentage is mass ratio except that other has indicating among the present invention, and product of the present invention adopts such method to prepare:
1. the preparation of selection of probio and culture medium
A bacterium---lactobacillus acidophilus CICC6074 (Lactobacillus acidophilus), culture medium is: peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, glucose 20 grams, dipotassium hydrogen phosphate 2 grams, sodium acetate 5 grams, ammonium citrate 2 grams, magnesium sulfate 0.2 gram, manganese sulfate 0.2 gram, 1 milliliter of Tween 80,1000 milliliters in water, pH6.2-6.6.
Add yeast extract 0.5, aseptic calcium carbonate 0.6 in 5 ° of B é brewer's worts of B bacterium---Lactobacillus plantarum CICC6002 (Lactobacillus plantarum).
C bacterium---Lactococcus lactis CICC23610 (Lactobacillus lactis) peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, glucose 20 grams, dipotassium hydrogen phosphate 2 grams, sodium acetate 5 grams, ammonium citrate 2 grams, magnesium sulfate 0.2 gram, manganese sulfate 0.2 gram, 1 milliliter of Tween 80,1000 milliliters in water, agar 1.5～2.0%, pH6.2-6.6.
Add yeast extract 0.5, aseptic calcium carbonate 0.6 in 5 ° of B é brewer's worts of D bacterium---Lactobacillus rhamnosus CICC6001 (Lactobacillus rhamnosus).
E bacterium---bifidobacterium bifidum CICC6168 (Bifidobacterium bifidum)
Casein peptone 10g, meat soup extract 5g, yeast extract 5g, soy peptone 5g, glucose 10g, Tween 80 1ml, K
2HPO
42g/L, MgSO
4* 7H
2O 0.2g/L, MnSO
4X H
2O 0.05g/L, NaCl 5g, salting liquid 4ml, resazurin (25mg/100ml) 4ml, distilled water 950ml, culture medium boil to boiling, add cysteine, behind the accent pH to 6.8, at CO
2Following cooling.Salting liquid: CaCl
2* 2H
2O 0.25g/L, KH
2PO
41g/L, K
2HPO
41g/L, MgSO
4* 7H
2O 0.5g/L, NaHCO
310g/L, NaCl 2g/L
2. the preparation process of beneficial flora
2.1 the activation of freeze-dried vaccine powder
Measure 0.9% physiological saline 10ml respectively in vitro in 5, be cooled to 30 ℃ in 20 minutes through 121 ℃ of sterilizations, freeze-dried vaccine powder in the 5 strains of lactic acid bacteria peace bottle all poured under germ-free condition in 0.9% the physiological saline, concussion makes its dissolving, static activation is 30 minutes in 30 ℃ of insulating boxs, and is standby.
2.2 enlarging, probio cultivates
2.2.1 the preparation of mother culture
Measure 5 each 200ml of probiotics culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, press 10% of culture volume and inoculate activated bacterial classification in 2.1 steps, static cultivation is 24 hours in 30 ℃ of anaerobic box, as mother culture.
2.2.2 probio spreads cultivation
At first dispose 10% defatted milk emulsion, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, and by 2% volume ratio inoculation mother culture, 30 ℃ of anaerobism were cultivated 30-36 hour, detected 5 strains of lactic acid bacteria zymotic fluid viable counts, each zymotic fluid viable count 〉=10 respectively
9Individual/ml, treat as and be fermenting-ripening, if viable count＜10
9Individual/ml, continue to cultivate, until reaching 10
9Individual/ml, then under germ-free condition 5000 rev/mins centrifugal 20 minutes, discard supernatant, it is stand-by to stay the bacterium mud drum to bury.
2.3 microecological microbial agent embedding
Get every kind respectively and treat embedding probiotics bacterial mud 10 grams, taking by weighing fineness then is 200 purpose edible mushroom Ji matsutake powder, 4 grams, with contain 2.5% sodium alginate and mix with the colloid mixture 40g of 0.5% flaxseed gum, 200r/min carries out stirring the first time 20min, mixture is slowly poured in the triangular flask that fills 1.5 times of volume edible oils, 400r/min carries out stirring the second time 20min, adds 1.5%CaCl
2Solution calcification 3h, with sterile saline detergent gel ball three times, glueballs is placed 0.6%, be carried out to film reaction in the chitosan solution of pH=5.5, take out behind the 15min, wash the 30min that liquefies in the citric acid solution that is placed on 55mM with sterile saline, the microbial inoculum microcapsules that must wet after the sterile saline flushing, the centrifugal 10min of 150r/min collects microcapsules, precooling, and last freeze drying obtains the microecological microbial agent after the embedding.
2.4 microecological microbial agent is composite
By each microecological microbial agent weight, get A bacterium 2-4 part, B bacterium 1-3 part, C bacterium 3-5 part, D bacterium 2-3 part, the abundant mixing final vacuum packing of E bacterium 5-7 part.
3. effect
Prove the effect of patent of the present invention below by concrete experiment
Body weight is divided into 1 group, test period 20 days per 10 of the northeastern tiger cub of 8-10 kilogram.
Table 1 beneficial flora is to the action effect of northeastern tiger cub
The daily gain difference of three test group is little, and ill ratio is starkly lower than control group, and microecological microbial agent of the present invention 10 grams of only need feeding every day concerning the northeastern tiger cub are described, can obviously improve immunity of organisms.
(4) specific embodiment
For a more detailed description below in conjunction with specific embodiment to the present invention:
Embodiment one:
5 probiotics that the present invention adopts are all purchased in Chinese industrial microorganism fungus kind preservation center, be respectively: lactobacillus acidophilus CICC 6074, Lactobacillus plantarum CICC 6002, Lactococcus lactis CICC23610, Lactobacillus rhamnosus CICC6001, bifidobacterium bifidum CICC6168 among the present invention abbreviates above-mentioned bacterial strains as A bacterium, B bacterium, C bacterium, D bacterium, E bacterium.
1. the preparation of selection of probio and culture medium
A bacterium---lactobacillus acidophilus CICC6074 (Lactobacillus acidophilus), culture medium is: peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, glucose 20 grams, dipotassium hydrogen phosphate 2 grams, sodium acetate 5 grams, ammonium citrate 2 grams, magnesium sulfate 0.2 gram, manganese sulfate 0.2 gram, 1 milliliter of Tween 80,1000 milliliters in water, pH6.2-6.6.
Add yeast extract 0.5, aseptic calcium carbonate 0.6 in 5 ° of B é brewer's worts of B bacterium---Lactobacillus plantarum CICC6002 (Lactobacillus plantarum).
C bacterium---Lactococcus lactis CICC23610 (Lactobacillus lactis) peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, glucose 20 grams, dipotassium hydrogen phosphate 2 grams, sodium acetate 5 grams, ammonium citrate 2 grams, magnesium sulfate 0.2 gram, manganese sulfate 0.2 gram, 1 milliliter of Tween 80,1000 milliliters in water, agar 1.5～2.0%, pH6.2-6.6.
Add yeast extract 0.5, aseptic calcium carbonate 0.6 in 5 ° of B é brewer's worts of D bacterium---Lactobacillus rhamnosus CICC6001 (Lactobacillus rhamnosus).
E bacterium---bifidobacterium bifidum CICC6168 (Bifidobacterium bifidum)
Casein peptone 10g, meat soup extract 5g, yeast extract 5g, soy peptone 5g, glucose 10g, Tween 80 1ml, K
2HPO
42g/L, MgSO
4* 7H
2O 0.2g/L, MnSO
4X H
2O 0.05g/L, NaCl 5g, salting liquid 4ml, resazurin (25mg/100ml) 4ml, distilled water 950ml, culture medium boil to boiling, add cysteine, behind the accent pH to 6.8, at CO
2Following cooling.Salting liquid: CaCl
2* 2H
2O 0.25g/L, KH
2PO
41g/L, K
2HPO
41g/L, MgSO
4* 7H
2O 0.5g/L, NaHCO
310g/L, NaCl 2g/L
2. the preparation process of beneficial flora
2.1 the activation of freeze-dried vaccine powder
Measure 0.9% physiological saline 10mi respectively in vitro in 5, be cooled to 30 ℃ in 20 minutes through 121 ℃ of sterilizations, freeze-dried vaccine powder in the 5 strains of lactic acid bacteria peace bottle all poured under germ-free condition in 0.9% the physiological saline, concussion makes its dissolving, static activation is 30 minutes in 30 ℃ of insulating boxs, and is standby.
2.2 enlarging, probio cultivates
2.2.1 the preparation of mother culture
Measure 5 each 200ml of probiotics culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, press 10% of culture volume and inoculate activated bacterial classification in 2.1 steps, static cultivation is 24 hours in 30 ℃ of anaerobic box, as mother culture.
2.2.2 probio spreads cultivation
At first dispose 10% defatted milk emulsion, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, and by 2% volume ratio inoculation mother culture, 30 ℃ of anaerobism were cultivated 30-36 hour, detected 5 strains of lactic acid bacteria zymotic fluid viable counts, each zymotic fluid viable count 〉=10 respectively
9Individual/ml, treat as and be fermenting-ripening, if viable count＜10
9Individual/ml, continue to cultivate, until reaching 10
9Individual/ml, then under germ-free condition 5000 rev/mins centrifugal 20 minutes, discard supernatant, it is stand-by to stay the bacterium mud drum to bury.
2.3 microecological microbial agent embedding
Get every kind respectively and treat embedding probiotics bacterial mud 10 grams, taking by weighing fineness then is 200 purpose edible mushroom Ji matsutake powder, 4 grams, with contain 2.5% sodium alginate and mix with the colloid mixture 40g of 0.5% flaxseed gum, 200r/min carries out stirring the first time 20min, mixture is slowly poured in the triangular flask that fills 1.5 times of volume edible oils, 400r/min carries out stirring the second time 20min, adds 1.5%CaCl
2Solution calcification 3h, with sterile saline detergent gel ball three times, glueballs is placed 0.6%, be carried out to film reaction in the chitosan solution of pH=5.5, take out behind the 15min, wash the 30min that liquefies in the citric acid solution that is placed on 55mM with sterile saline, the microbial inoculum microcapsules that must wet after the sterile saline flushing, the centrifugal 10min of 150r/min collects microcapsules, precooling, and last freeze drying obtains the microecological microbial agent after the embedding.
2.4 microecological microbial agent is composite
By each microecological microbial agent weight, get 2 parts of A bacterium, 1 part of B bacterium, 3 parts of C bacterium, 2 parts of D bacterium, 5 parts of abundant mixing final vacuum packings of E bacterium.
Embodiment two:
1. the preparation of selection of probio and culture medium
A bacterium---lactobacillus acidophilus CICC6074 (Lactobacillus acidophilus), culture medium is: peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, glucose 20 grams, dipotassium hydrogen phosphate 2 grams, sodium acetate 5 grams, ammonium citrate 2 grams, magnesium sulfate 0.2 gram, manganese sulfate 0.2 gram, 1 milliliter of Tween 80,1000 milliliters in water, pH6.2-6.6.
Add yeast extract 0.5, aseptic calcium carbonate 0.6 in 5 ° of B é brewer's worts of B bacterium---Lactobacillus plantarum CICC6002 (Lactobacillus plantarum).
C bacterium---Lactococcus lactis CICC23610 (Lactobacillus lactis) peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, glucose 20 grams, dipotassium hydrogen phosphate 2 grams, sodium acetate 5 grams, ammonium citrate 2 grams, magnesium sulfate 0.2 gram, manganese sulfate 0.2 gram, 1 milliliter of Tween 80,1000 milliliters in water, agar 1.5～2.0%, pH6.2-6.6.
Add yeast extract 0.5, aseptic calcium carbonate 0.6 in 5 ° of B é brewer's worts of D bacterium---Lactobacillus rhamnosus CICC6001 (Lactobacillus rhamnosus).
E bacterium---bifidobacterium bifidum CICC6168 (Bifidobacterium bifidum)
Casein peptone 10g, meat soup extract 5g, yeast extract 5g, soy peptone 5g, glucose 10g, Tween 80 1ml, K
2HPO
42g/L, MgSO
4* 7H
2O 0.2g/L, MnSO
4X H
2O 0.05g/L, NaCl 5g, salting liquid 4ml, resazurin (25mg/100ml) 4ml, distilled water 950ml, culture medium boil to boiling, add cysteine, behind the accent pH to 6.8, at CO
2Following cooling.Salting liquid: CaCl
2* 2H
2O 0.25g/L, KH
2PO
41g/L, K
2HPO
41g/L, MgSO
4* 7H
2O 0.5g/L, NaHCO
310g/L, NaCl 2g/L
2. the preparation process of beneficial flora
2.1 the activation of freeze-dried vaccine powder
Measure 0.9% physiological saline 10ml respectively in vitro in 5, be cooled to 30 ℃ in 20 minutes through 121 ℃ of sterilizations, freeze-dried vaccine powder in the 5 strains of lactic acid bacteria peace bottle all poured under germ-free condition in 0.9% the physiological saline, concussion makes its dissolving, static activation is 30 minutes in 30 ℃ of insulating boxs, and is standby.
2.2 enlarging, probio cultivates
2.2.1 the preparation of mother culture
Measure 5 each 200ml of probiotics culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, press 10% of culture volume and inoculate activated bacterial classification in 2.1 steps, static cultivation is 24 hours in 30 ℃ of anaerobic box, as mother culture.
2.2.2 probio spreads cultivation
At first dispose 10% defatted milk emulsion, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, and by 2% volume ratio inoculation mother culture, 30 ℃ of anaerobism were cultivated 30-36 hour, detected 5 strains of lactic acid bacteria zymotic fluid viable counts, each zymotic fluid viable count 〉=10 respectively
9Individual/ml, treat as and be fermenting-ripening, if viable count＜10
9Individual/ml, continue to cultivate, until reaching 10
9Individual/ml, then under germ-free condition 5000 rev/mins centrifugal 20 minutes, discard supernatant, it is stand-by to stay the bacterium mud drum to bury.
2.3 microecological microbial agent embedding
Get every kind respectively and treat embedding probiotics bacterial mud 10 grams, taking by weighing fineness then is 200 purpose edible mushroom Ji matsutake powder, 4 grams, with contain 2.5% sodium alginate and mix with the colloid mixture 40g of 0.5% flaxseed gum, 200r/min carries out stirring the first time 20min, mixture is slowly poured in the triangular flask that fills 1.5 times of volume edible oils, 400r/min carries out stirring the second time 20min, adds 1.5%CaCl
2Solution calcification 3h, with sterile saline detergent gel ball three times, glueballs is placed 0.6%, be carried out to film reaction in the chitosan solution of pH=5.5, take out behind the 15min, wash the 30min that liquefies in the citric acid solution that is placed on 55mM with sterile saline, the microbial inoculum microcapsules that must wet after the sterile saline flushing, the centrifugal 10min of 150r/min collects microcapsules, precooling, and last freeze drying obtains the microecological microbial agent after the embedding.
2.4 microecological microbial agent is composite
By each microecological microbial agent weight, get 4 parts of A bacterium, 3 parts of B bacterium, 5 parts of C bacterium, 3 parts of D bacterium, 7 parts of abundant mixing final vacuum packings of E bacterium.
Claims (1)
1. the preparation method of a siberian tiger intestinal tract probiotics, it is characterized in that: the preparation lactobacillus acidophilus, Lactobacillus plantarum, Lactococcus lactis, Lactobacillus rhamnosus, bifidobacterium bifidum 5 probiotics zymotic fluids, after 5000 rev/mins, discard supernatant then, get every kind of probiotics bacterial mud 10 grams respectively, adding fineness is 200 purpose edible mushroom Ji matsutake powder, 4 grams, with contain 2.5% sodium alginate and mix with the colloid mixture 40g of 0.5% flaxseed gum, 200r/min carries out stirring the first time 20min, mixture is poured in the triangular flask that fills 1.5 times of volume edible oils, 400r/min carries out stirring the second time 20min, adds 1.5%CaCl
2Solution calcification 3h, with sterile saline detergent gel ball three times, glueballs is placed 0.6%, take out behind the film formation reaction 15min in the chitosan solution of pH=5.5, wash the 30min that liquefies in the citric acid solution that is placed on 55mM with sterile saline, the probio that must wet after the sterile saline flushing, the centrifugal 10min of 150r/min collects probio, precooling, last freeze drying obtains beneficial bacteria of intestinal tract, through the composite acquisition beneficial bacteria of intestinal tract group of different probiotic powders.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010194464 CN101869219A (en) | 2010-06-08 | 2010-06-08 | Preparation method of siberian tiger intestinal tract probiotics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010194464 CN101869219A (en) | 2010-06-08 | 2010-06-08 | Preparation method of siberian tiger intestinal tract probiotics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101869219A true CN101869219A (en) | 2010-10-27 |
Family
ID=42994449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010194464 Pending CN101869219A (en) | 2010-06-08 | 2010-06-08 | Preparation method of siberian tiger intestinal tract probiotics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101869219A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318806A (en) * | 2011-08-11 | 2012-01-18 | 黑龙江顺康蔬菜加工有限公司 | Preparation method of probiotics fermented pumpkin and carrot vegetable powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101277708A (en) * | 2005-10-06 | 2008-10-01 | 雀巢技术公司 | Probiotic enterococci for improved immunity |
| CN101401638A (en) * | 2008-10-17 | 2009-04-08 | 东北农业大学 | Method for preparing edible mushroom synbiotics microcapsule |
-
2010
- 2010-06-08 CN CN 201010194464 patent/CN101869219A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101277708A (en) * | 2005-10-06 | 2008-10-01 | 雀巢技术公司 | Probiotic enterococci for improved immunity |
| CN101401638A (en) * | 2008-10-17 | 2009-04-08 | 东北农业大学 | Method for preparing edible mushroom synbiotics microcapsule |
Non-Patent Citations (1)
| Title |
|---|
| 《养殖技术顾问》 20091231 王莹莹等 东北虎代谢性菌群失调综合症的分析 第93页 1 , 第3期 2 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318806A (en) * | 2011-08-11 | 2012-01-18 | 黑龙江顺康蔬菜加工有限公司 | Preparation method of probiotics fermented pumpkin and carrot vegetable powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102399733B (en) | Lactobacillus johnsonii, microbial inoculum, application and premix thereof | |
| CN100342005C (en) | Process for preparing high actived alctic acid bacteria product | |
| CN101278702B (en) | Novel microbial feed additive and method of producing the same | |
| CN103627656B (en) | A kind of clostridium butyricum and bacillus coagulans mixed culture solid state fermentation method | |
| CN108783462A (en) | A kind of industrial process of beneficial bacteria of intestinal tract preparation | |
| CN102318806B (en) | Preparation method of probiotics fermented pumpkin and carrot vegetable powder | |
| CN101092603B (en) | Method for producing conjugated linoleic acid, and dedicated bacterial strain | |
| US20120034198A1 (en) | Carriers for storage and delivery of biologics | |
| CN103704719B (en) | A kind of preparation method of the probiotic microcapsule with high viable count | |
| CN102174450B (en) | Lactobacillus plantarum for resisting helicobacter pylori infection and application thereof | |
| CN102742728A (en) | Compound probiotic feed additive for livestock and poultry, and premix, concentrate feed and mixed feed containing same | |
| CN101401638A (en) | Method for preparing edible mushroom synbiotics microcapsule | |
| CN101971920B (en) | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof | |
| CN107312732B (en) | Probiotic feed additive | |
| CN103766486B (en) | A kind of milky-drinks containing bifidus bacillus embedding pearl and preparation method thereof | |
| US20180187274A1 (en) | Lactobacillus paracasei for the production of conjugated linoleic acid, nutritional and pharmaceutical preparations containing it and uses thereof | |
| CN104371958A (en) | Method for increasing biomass by virtue of mixed cultivation of enterococcus faecium and bacillus subtilis | |
| CN104432001A (en) | Edible composition and use thereof | |
| CN103300208A (en) | Production method of live lactobacillus casei preparation | |
| CN102334684A (en) | Preparation method of microbial product with specific function | |
| CN102210454B (en) | Microbial compound preparation and preparation method thereof | |
| CN104095148B (en) | Preparation method of products containing animal intestinal beneficial bacteria | |
| CN103283974A (en) | A method for producing a probiotic agent of live Lactobacillus bulgaricus | |
| CN105559087A (en) | Probiotic product containing sialic acid and preparation method of probiotic product | |
| CN102524794A (en) | Preparation method of serum cholesterol reduction Kluyveromyces marxianus freeze-dried powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20101027 |