CN101865836A - Quality control method of Tibetan medicine lycium ruthenicum - Google Patents
Quality control method of Tibetan medicine lycium ruthenicum Download PDFInfo
- Publication number
- CN101865836A CN101865836A CN201010200389A CN201010200389A CN101865836A CN 101865836 A CN101865836 A CN 101865836A CN 201010200389 A CN201010200389 A CN 201010200389A CN 201010200389 A CN201010200389 A CN 201010200389A CN 101865836 A CN101865836 A CN 101865836A
- Authority
- CN
- China
- Prior art keywords
- content
- sample solution
- solution
- lycium ruthenicum
- tibetan medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a quality control method of Tibetan medicine lycium ruthenicum. The method takes measurements of total anthocyanin content, proanthocyanidin content and total polyphenol content as quality control indexes, has the advantages of less reagent content, simple and convenient operation, high sensitivity and precision, good stability, and the like and is capable of realizing the effective evaluation on the health care function of the Tibetan medicine lycium ruthenicum.
Description
Technical field
The present invention relates to a kind of Tibetan medicine method of quality control, relate in particular to quality control method of Tibetan medicine lycium ruthenicum.
Background technology
Tibetan medicine lycium ruthenicum is the ripening fruits of Solanaceae Lycium plant black fruit lyceum (Lycium ruthenicum Murr.), and stronger inoxidizability is arranged, and is nontoxic, is applied to medicine, food.
Mainly contain anthocyanin class material, procyanidin and total polyphenols in the Tibetan medicine lycium ruthenicum.Wherein the anthocyanin class material is one of lyochromes, extensively be present in the flower and fruit of plant, belong to the flavonoids in the phenolic compound, have the interior free yl of removing, anti-oxidant, anti-sudden change is anticancer, antihyperglycemic is active, reducing blood lipid and prevent and treat effect such as atherosclerotic; Procyanidin (Proanthocyanidins or Procyanidins, be called for short PC, translate proanthocyanidin, preceding anthocyanidin etc. again) be the general name of the big class natural polyphenol compound that extensively exists in the plant, have the human free radical of removing, anti-oxidant, ultraviolet injury resistant, anti-mutation, enzyme inhibition isoreactivity; Total polyphenols has the free radical of removing, anti arteriosclerosis, reducing blood lipid, oxidation resistant function.
But Tibetan medicine lycium ruthenicum does not also have complete method of quality control at present, is unfavorable for guaranteeing the Tibetan medicine lycium ruthenicum effectiveness of application.
Summary of the invention
Technical matters to be solved by this invention provide a kind of simply, quality control method of Tibetan medicine lycium ruthenicum fast and accurately.
For addressing the above problem, a kind of quality control method of Tibetan medicine lycium ruthenicum of the present invention is characterized in that: this method with total anthocyanin content, procyanidin content and total polyphenols Determination on content as quality control index; Wherein
---the assay method of total anthocyanin content is: (1) preparation sample solution: 0.03～3g Tibetan medicine lycium ruthenicum is placed bottle, add 10～100ml hydrochloric acid-ethanolic solution, dilute 5～25 times promptly after being incubated 1～36 hour under 15～80 ℃ of temperature; (2) working sample solution absorbance value: is 450～600nm place working sample solution absorbance value with ultraviolet-visible spectrophotometer at wavelength; (3) total anthocyanin content in the calculation sample solution;
---the assay method of procyanidin content is: 1. prepare reference substance solution: methyl alcohol is joined in the catechin reference substance, make behind the solution that every 1ml contains catechin 0.1mg promptly; 2. preparation standard curve: respectively with 0.0,0.5,1,1.5,2, to 25ml, the standard that promptly gets concentration and be 0.01～0.5mg/ml is used liquid with methanol constant volume for the reference substance solution of 2.5ml; 3. prepare sample solution: in 0.01～2g Tibetan medicine lycium ruthenicum, add methyl alcohol, after 10～40 minutes, be cooled to room temperature, and, shake up, promptly with methanol constant volume to 5～250ml through sonicated; 4. procyanidin content in the working sample solution: in 0.1～2ml sample solution, add 1～5ml vanillic aldehyde methanol solution, 0.5～2ml concentrated hydrochloric acid successively, mix, behind the 10～20mim that develops the color under the room temperature, wavelength be 400～600nm place colorimetric promptly;
---total polyphenols Determination on content method is: (a) preparation reference substance solution: add entry in 1～10mg gallic acid reference substance, be settled to 2～25ml behind ultrasonic dissolution, promptly get the solution that every 1ml contains gallic acid 0.25g; (b) preparation standard curve: respectively with 0.0,0.5,1.0,1.5,2.0, the reference substance solution water of 2.5ml is settled to 50ml, shake up, promptly; (c) preparation sample solution: 0.01～2g Tibetan medicine lycium ruthenicum was added 5～50ml ethanol ultrasonic 30 minutes, and 1～50 times of water stepwise dilution, promptly get sample solution; (d) working sample solution absorbance value: in sample solution, add forint-phenol developer, shake up the back and add sodium carbonate liquor, be settled to 10ml; Solution with adding forint-phenol developer and sodium carbonate is blank, is 300～800nm place working sample solution absorbance value with ultraviolet-visible spectrophotometer at wavelength; (e) total polyphenols content in the calculation sample solution.
The mass concentration of hydrochloric acid is 0.01～1% in the step in the assay method of described total anthocyanin content (1), and the mass concentration of ethanol is 50～90%.
The 3. middle sonicated condition of step in the assay method of described procyanidin content is that power is 250～400W, frequency 35～60KHz.
Step in the assay method of the described procyanidin content 4. mass concentration of middle vanillic aldehyde methanol solution is 4%.
The mass concentration of sodium carbonate liquor is 20% in the step (d) in the described total polyphenols Determination on content method.
The present invention compared with prior art has the following advantages:
1, because the present invention adopts total anthocyanin, procyanidin and total polyphenols in the spectrophotometry Tibetan medicine lycium ruthenicum, and this method has few, simple to operation, highly sensitive, the advantages such as precision is high, good stability of agents useful for same amount, therefore, suitable anthocyanin, procyanidin and the total polyphenols of measuring Tibetan medicine lycium ruthenicum, thus realize Tibetan medicine lycium ruthenicum is carried out the effective evaluation of health care.
2, after the present invention being carried out precision experiment, stability experiment, recovery experiment, the present invention has higher precision, at 8 hours internal stabilities well, accurately and reliably as can be seen, can realize Tibetan medicine
Black fruit lyceum quality control effectively (referring to table 1, table 2, table 3).
Table 1: precision experiment (frequency n=5)
| The mensuration project | Average content | ??RSD(％) |
| Anthocyanin | ??0.01g/g | ??1.12 |
| Procyanidin | ??1.6g/100g | ??1.34 |
| Total polyphenols | ??4g/100g | ??1.24 |
Table 2: stability experiment (t=8h)
| The mensuration project | Average content | ??RSD(％) |
| Anthocyanin | ??0.011g/g | ??1.23 |
| Procyanidin | ??1.58g/100g | ??2.44 |
| Total polyphenols | ??3.96g/100g | ??1.76 |
Table 3: recovery experiment
| The mensuration project | Average recovery rate (%) | ??RSD(％) |
| Anthocyanin | ??100.34 | ??1.67 |
| Procyanidin | ??102.54 | ??1.42 |
| Total polyphenols | ??98.87 | ??1.26 |
3, the present invention is simple, quick, is easy to promote.
Embodiment
The present invention will be further described below in conjunction with embodiment, and following embodiment only is used to illustrate the present invention, and is not limitation of the present invention.
1 one kinds of quality control method of Tibetan medicine lycium ruthenicum of embodiment, this method with total anthocyanin content, procyanidin content and total polyphenols Determination on content as quality control index.Wherein
---the assay method of total anthocyanin content is:
(1) preparation sample solution: get the 0.03g Tibetan medicine lycium ruthenicum, add 10ml0.1% hydrochloric acid-80% ethanolic solution, dilute 5 times after 24 hours promptly in insulation under 25 ℃ of temperature.
(2) working sample solution absorbance value: is that 540nm place working sample solution absorbance value is 1.79 with ultraviolet-visible spectrophotometer at wavelength.
(3) total anthocyanin content in the calculation sample solution.
Total anthocyanin content (mg/g)=(A * MW * DF)/b, A is an absorbance in the formula; MW is relative molecular weight (getting 449.2); DF is an extension rate; B is molar absorptivity (getting 26900), and the content that obtains total anthocyanin of Tibetan medicine lycium ruthenicum is 10mg/g.
---the measuring principle of procyanidin content: procyanidin is the polymkeric substance that contains catechin and epicatechin unit.Procyanidin itself is colourless, with the vanillic aldehyde reaction, can produce wine-colored anthocyanidin ion in acid medium.
Assay method is:
1. prepare reference substance solution: methyl alcohol is joined in the catechin reference substance, make behind the solution that every 1ml contains catechin 0.1mg promptly.
2. preparation standard curve: respectively with 0.0,0.5,1,1.5,2, the reference substance solution of 2.5ml puts in the 25ml measuring bottle, methanol constant volume shakes up to 25ml, the standard that promptly gets concentration and be 0.01～0.5mg/ml is used liquid.With methyl alcohol is blank, measures absorbance A at the 500nm wavelength, with concentration C absorbance A is returned, and getting regression equation is A=5.4012C+0.0014, Γ=0.9997.
3. prepare sample solution: get Tibetan medicine lycium ruthenicum 0.1g, the accurate title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 25ml that adds, close plug claims to decide weight, sonicated (power 250W, frequency 35KHz) after 10 minutes, is cooled to room temperature, claims to decide weight again, supply the weight that subtracts mistake with methyl alcohol, be settled to 25ml, shake up, promptly.
4. procyanidin content in the working sample solution: with masking foil that the test tube parcel is tight, only stay the mouth of pipe to be used for application of sample.Sample thief 1ml adds in the test tube, and the 4% vanilla formalin that adds 3ml is again mixed, and adds the concentrated hydrochloric acid of 1.5ml then, thorough mixing, and color development at room temperature 10 minutes is a 500nm place colorimetric at wavelength at last.
The assay result: as calculated, the Tibetan medicine lycium ruthenicum procyanidin content is 1.6g/100g.
---total polyphenols Determination on content method is:
(a) preparation reference substance solution: in 1～10mg gallic acid reference substance, add entry, after ultrasonic (power 400W, frequency 57KHz) dissolving, be settled to 2～25ml, promptly get the solution that every 1ml contains gallic acid 0.25g.
(b) preparation standard curve: respectively with 0.0,0.5,1.0,1.5,2.0, the reference substance solution of 2.5ml places the 50ml measuring bottle, water is settled to 50ml, shakes up, promptly.Measure absorbance A at the 760nm wavelength, with concentration C absorbance A is returned, getting regression equation is A=3.0940C+0.0178, Γ=0.9994.
(c) preparation sample solution: the 0.1g Tibetan medicine lycium ruthenicum was added 25ml ethanol ultrasonic 30 minutes, and 25 times of water stepwise dilutions, promptly get sample solution;
(d) working sample solution absorbance value: extracting sample solution 1ml, put in the 10ml measuring bottle, add water 6ml, add forint-phenol developer 0.5ml, shake up, add 20% sodium carbonate liquor 1.5ml in 10 minutes, be settled to scale 10ml, shake up.Solution with adding forint-phenol developer and sodium carbonate is blank, is 760nm place working sample solution absorbance value with ultraviolet-visible spectrophotometer at wavelength.
Forint-phenol developer: take by weighing 10g sodium tungstate and 2.5g sodium molybdate, be dissolved in the 250ml Backflow bottle with 70ml distilled water, add 5ml phosphoric acid and 10ml concentrated hydrochloric acid, mixing, little boiling refluxed 2 hours, add 1.5g lithium sulfate, 5ml distilled water and 3 bromine waters, opening boiling 15 minutes (till waving to the greatest extent to bromine water) is cooled to room temperature, is settled to 100ml, filter, put preserve in the brown bottle standby.Adding 1 times of distilled water diluting during use gets final product.
The preparation of 20% sodium carbonate liquor: take by weighing the 20g natrium carbonicum calcinatum, put in the 100ml volumetric flask, add 80ml water ultrasonic (power 400W, frequency 57KHz) dissolving, be settled to 100ml after being cooled to room temperature, shake up, filter, promptly.
(e) total polyphenols content in the calculation sample solution.
Content * extension rate * sample quality after total polyphenols content (g/g)=dilution
-1, obtaining Tibetan medicine lycium ruthenicum total polyphenols content is 4g/100g.
2 one kinds of quality control method of Tibetan medicine lycium ruthenicum of embodiment, this method with total anthocyanin content, procyanidin content and total polyphenols Determination on content as quality control index.Wherein
---the assay method of total anthocyanin content is:
(1) preparation sample solution: get the 0.3g Tibetan medicine lycium ruthenicum and place bottle, add 50ml0.01% hydrochloric acid-50% ethanolic solution, dilute 10 times in insulation under 15 ℃ of temperature after 36 hours and promptly get (2) working sample solution absorbance value: is that 450nm place working sample solution absorbance value is 0.33 with ultraviolet-visible spectrophotometer at wavelength.
(3) total anthocyanin content in the calculation sample solution.
Total anthocyanin content (mg/g)=(A * MW * DF)/b, A is an absorbance in the formula; MW is relative molecular weight (getting 449.2); DF is an extension rate; B is molar absorptivity (getting 26900), and the content that obtains total anthocyanin of Tibetan medicine lycium ruthenicum is 9.6mg/g.
---the assay method of procyanidin content is:
1. prepare reference substance solution, 2. the preparation standard curve is with embodiment 1.
3. prepare sample solution: get Tibetan medicine lycium ruthenicum 0.01g, the accurate title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 5ml that adds, close plug claims to decide weight, sonicated (power 300W, frequency 50KHz) after 25 minutes, is cooled to room temperature, claims to decide weight again, supply the weight that subtracts mistake with methyl alcohol, be settled to 5ml, shake up, promptly.
4. procyanidin content in the working sample solution: with masking foil that the test tube parcel is tight, only stay the mouth of pipe to be used for application of sample.Sample thief 0.1ml adds in the test tube, and the 4% vanilla formalin that adds 1ml is again mixed, and adds the concentrated hydrochloric acid of 0.5ml then, thorough mixing, and color development at room temperature 15 minutes is a 400nm place colorimetric at wavelength at last.
The assay result: as calculated, the Tibetan medicine lycium ruthenicum procyanidin content is 1.54g/100g.
---total polyphenols Determination on content method is: (a) preparation reference substance solution, (b) preparation standard curve are with embodiment 1.
(c) preparation sample solution: the 0.01g Tibetan medicine lycium ruthenicum was added 5ml ethanol ultrasonic 30 minutes, and 1 times of water stepwise dilution, promptly get sample solution;
(d) working sample solution absorbance value: extracting sample solution 1ml, put in the 10ml measuring bottle, add water 6ml, add forint-phenol developer 0.5ml, shake up, add 20% sodium carbonate liquor 1.5ml in 10 minutes, be settled to scale 10ml, shake up.Solution with adding forint-phenol developer and sodium carbonate is blank, is 300nm place working sample solution absorbance value with ultraviolet-visible spectrophotometer at wavelength.
The preparation of forint-phenol developer and 20% sodium carbonate liquor is with embodiment 1.
(e) total polyphenols content in the calculation sample solution.
Content * extension rate * sample quality after total polyphenols content (g/g)=dilution
-1, obtaining Tibetan medicine lycium ruthenicum total polyphenols content is 3.8g/100g.
3 one kinds of quality control method of Tibetan medicine lycium ruthenicum of embodiment, this method with total anthocyanin content, procyanidin content and total polyphenols Determination on content as quality control index.Wherein
---the assay method of total anthocyanin content is:
(1) preparation sample solution: get the 3g Tibetan medicine lycium ruthenicum is placed bottle, add 100ml1% hydrochloric acid-90% ethanolic solution, dilute 25 after 1 hour in insulation under 80 ℃ of temperature and promptly get (2) working sample solution absorbance value: is that 600nm place working sample solution absorbance value is 0.67 with ultraviolet-visible spectrophotometer at wavelength.
(3) total anthocyanin content in the calculation sample solution.
Total anthocyanin content (mg/g)=(A * MW * DF)/b, A is an absorbance in the formula; MW is relative molecular weight (getting 449.2); DF is an extension rate; B is molar absorptivity (getting 26900), and the content that obtains total anthocyanin of Tibetan medicine lycium ruthenicum is 9.7mg/g.
---the assay method of procyanidin content is:
1. prepare reference substance solution, 2. the preparation standard curve is with embodiment 1.
3. prepare sample solution: get Tibetan medicine lycium ruthenicum 2g, the accurate title, decide, and puts in the tool plug conical flask, the accurate methyl alcohol 250ml that adds, close plug claims to decide weight, sonicated (power 400W, frequency 60KHz) after 40 minutes, is cooled to room temperature, claims to decide weight again, supply the weight that subtracts mistake with methyl alcohol, be settled to 250ml, shake up, promptly.
4. procyanidin content in the working sample solution: with masking foil that the test tube parcel is tight, only stay the mouth of pipe to be used for application of sample.Sample thief 2ml adds in the test tube, and the 4% vanilla formalin that adds 5ml is again mixed, and adds the concentrated hydrochloric acid of 2ml then, thorough mixing, and color development at room temperature 20 minutes is a 600nm place colorimetric at wavelength at last.
The assay result: as calculated, the Tibetan medicine lycium ruthenicum procyanidin content is 1.56g/100g.
---total polyphenols Determination on content method is:
(a) preparation reference substance solution, (b) preparation standard curve are with embodiment 1.
(c) preparation sample solution: the 2g Tibetan medicine lycium ruthenicum was added 50ml ethanol ultrasonic 30 minutes, and 50 times of water stepwise dilutions, promptly get sample solution;
(d) working sample solution absorbance value: extracting sample solution 1ml, put in the 10ml measuring bottle, add water 6ml, add forint-phenol developer 0.5ml, shake up, add 20% sodium carbonate liquor 1.5ml in 10 minutes, be settled to scale 10ml, shake up.Solution with adding forint-phenol developer and sodium carbonate is blank, is 800nm place working sample solution absorbance value with ultraviolet-visible spectrophotometer at wavelength.
The preparation of forint-phenol developer and 20% sodium carbonate liquor is with embodiment 1.
(e) total polyphenols content in the calculation sample solution.
Content * extension rate * sample quality after total polyphenols content (g/g)=dilution
-1, obtaining Tibetan medicine lycium ruthenicum total polyphenols content is 3.89g/100g.
Claims (5)
1. quality control method of Tibetan medicine lycium ruthenicum is characterized in that: this method with total anthocyanin content, procyanidin content and total polyphenols Determination on content as quality control index; Wherein
---the assay method of total anthocyanin content is: (1) preparation sample solution: 0.03～3g Tibetan medicine lycium ruthenicum is placed bottle, add 10～100ml hydrochloric acid-ethanolic solution, dilute 5～25 times promptly after being incubated 1～36 hour under 15～80 ℃ of temperature; (2) working sample solution absorbance value: is 450～600nm place working sample solution absorbance value with ultraviolet-visible spectrophotometer at wavelength; (3) total anthocyanin content in the calculation sample solution;
---the assay method of procyanidin content is: 1. prepare reference substance solution: methyl alcohol is joined in the catechin reference substance, make behind the solution that every 1ml contains catechin 0.1mg promptly; 2. preparation standard curve: respectively with 0.0,0.5,1,1.5,2, to 25ml, the standard that promptly gets concentration and be 0.01～0.5mg/ml is used liquid with methanol constant volume for the reference substance solution of 2.5ml; 3. prepare sample solution: in 0.01～2g Tibetan medicine lycium ruthenicum, add methyl alcohol, after 10～40 minutes, be cooled to room temperature, and, shake up, promptly with methanol constant volume to 5～250ml through sonicated; 4. procyanidin content in the working sample solution: in 0.1～2ml sample solution, add 1～5ml vanillic aldehyde methanol solution, 0.5～2ml concentrated hydrochloric acid successively, mix, behind the 10～20mim that develops the color under the room temperature, wavelength be 400～600nm place colorimetric promptly;
---total polyphenols Determination on content method is: (a) preparation reference substance solution: add entry in 1～10mg gallic acid reference substance, be settled to 2～25ml behind ultrasonic dissolution, promptly get the solution that every 1ml contains gallic acid 0.25g; (b) preparation standard curve: respectively with 0.0,0.5,1.0,1.5,2.0, the reference substance solution water of 2.5ml is settled to 50ml, shake up, promptly; (c) preparation sample solution: 0.01～2g Tibetan medicine lycium ruthenicum was added 5～50ml ethanol ultrasonic 30 minutes, and 1～50 times of water stepwise dilution, promptly get sample solution; (d) working sample solution absorbance value: in sample solution, add forint-phenol developer, shake up the back and add sodium carbonate liquor, be settled to 10ml; Solution with adding forint-phenol developer and sodium carbonate is blank, is 300～800nm place working sample solution absorbance value with ultraviolet-visible spectrophotometer at wavelength; (e) total polyphenols content in the calculation sample solution.
2. quality control method of Tibetan medicine lycium ruthenicum as claimed in claim 1 is characterized in that: the mass concentration of hydrochloric acid is 0.01～1% in the step in the assay method of described total anthocyanin content (1), and the mass concentration of ethanol is 50～90%.
3. quality control method of Tibetan medicine lycium ruthenicum as claimed in claim 1 is characterized in that: the 3. middle sonicated condition of the step in the assay method of described procyanidin content is that power is 250～400W, frequency 35～60KHz.
4. quality control method of Tibetan medicine lycium ruthenicum as claimed in claim 1 is characterized in that: the step in the assay method of the described procyanidin content 4. mass concentration of middle vanillic aldehyde methanol solution is 4%.
5. quality control method of Tibetan medicine lycium ruthenicum as claimed in claim 1 is characterized in that: the mass concentration of sodium carbonate liquor is 20% in the step (d) in the described total polyphenols Determination on content method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010200389A CN101865836A (en) | 2010-06-10 | 2010-06-10 | Quality control method of Tibetan medicine lycium ruthenicum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010200389A CN101865836A (en) | 2010-06-10 | 2010-06-10 | Quality control method of Tibetan medicine lycium ruthenicum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101865836A true CN101865836A (en) | 2010-10-20 |
Family
ID=42957641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010200389A Pending CN101865836A (en) | 2010-06-10 | 2010-06-10 | Quality control method of Tibetan medicine lycium ruthenicum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101865836A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198653A (en) * | 2014-09-17 | 2014-12-10 | 山东阿如拉药物研究开发有限公司 | Detection method for Lyciumruthenicum Murr |
| CN111214494A (en) * | 2020-03-10 | 2020-06-02 | 新疆奇沐医药研究院（有限公司） | Emulsifiable paste for treating oral diseases, preparation method thereof and method for measuring total polyphenol content in emulsifiable paste |
-
2010
- 2010-06-10 CN CN201010200389A patent/CN101865836A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198653A (en) * | 2014-09-17 | 2014-12-10 | 山东阿如拉药物研究开发有限公司 | Detection method for Lyciumruthenicum Murr |
| CN104198653B (en) * | 2014-09-17 | 2016-03-02 | 山东金诃药物研究开发有限公司 | The detection method of a kind of black matrimony vine |
| CN111214494A (en) * | 2020-03-10 | 2020-06-02 | 新疆奇沐医药研究院（有限公司） | Emulsifiable paste for treating oral diseases, preparation method thereof and method for measuring total polyphenol content in emulsifiable paste |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kaviarasan et al. | In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds | |
| Anandjiwala et al. | Evaluation of free radical scavenging activity of an ayurvedic formulation, Panchvalkala | |
| CN101858859A (en) | Quality control method of lycium ruthenicum pigment | |
| Popović et al. | Antioxidant capacity of cornelian cherry (Cornus mas L.)–Comparison between permanganate reducing antioxidant capacity and other antioxidant methods | |
| Li et al. | Comparative study of antioxidant activity of grape (Vitis vinifera) seed powder assessed by different methods | |
| CN101852729A (en) | Quality control method of black fruit lyceum juice | |
| Anissi et al. | A comparative study of the antioxidant scavenging activity of green tea, black tea and coffee extracts: A kinetic approach | |
| Adcock et al. | Advances in the use of acidic potassium permanganate as a chemiluminescence reagent: A review | |
| Malinauskiene et al. | Formaldehyde may be found in cosmetic products even when unlabelled | |
| Muzolf-Panek et al. | The influence of stereochemistry on the antioxidant properties of catechin epimers | |
| Manfrin et al. | Reactive oxygen species production from secondary organic aerosols: the importance of singlet oxygen | |
| Tabart et al. | Evaluation of spectrophotometric methods for antioxidant compound measurement in relation to total antioxidant capacity in beverages | |
| Opuwari et al. | In vivo effects of A spalathus linearis (rooibos) on male rat reproductive functions | |
| Janghel et al. | Micro determination of ascorbic acid using methyl viologen | |
| Pathy | Process for preparation of vitamin C and method for determination of vitamin C in tablets | |
| CN102141519B (en) | Method for detecting procyanidins content | |
| Kim et al. | Assessment and comparison of the antioxidant activities and nitrite scavenging activity of commonly consumed beverages in Korea | |
| Ricci et al. | Antioxidant activity of commercial food grade tannins exemplified in a wine model | |
| Moldovan et al. | The influence of storage on the total phenols content and antioxidant activity of the Cranberrybush (Viburnum opulus L.) fruits extract | |
| CN101865835A (en) | Nitraria sibirica pall juice quality control method | |
| Mura et al. | New insights into the antioxidant activity of hydroxycinnamic and hydroxybenzoic systems: Spectroscopic, electrochemistry, and cellular studies | |
| Khalil et al. | Content and antioxidant properties of processed Tualang honey (AgroMas®) collected from different regions in Malaysia | |
| Çekiç et al. | A colourimetric sensor for the simultaneous determination of oxidative status and antioxidant activity on the same membrane: N, N-Dimethyl-p-phenylene diamine (DMPD) on Nafion | |
| Díaz et al. | Valuable polyphenolic antioxidants from wine vinasses | |
| CN101865836A (en) | Quality control method of Tibetan medicine lycium ruthenicum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20101020 |