CN101858900B - Constant temperature and humidity thin-layer chromatography tank - Google Patents
Constant temperature and humidity thin-layer chromatography tank Download PDFInfo
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- CN101858900B CN101858900B CN 200910081467 CN200910081467A CN101858900B CN 101858900 B CN101858900 B CN 101858900B CN 200910081467 CN200910081467 CN 200910081467 CN 200910081467 A CN200910081467 A CN 200910081467A CN 101858900 B CN101858900 B CN 101858900B
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Abstract
The invention discloses a chromatography tank which comprises a temperature control device, a chromatography device contained in the temperature control device and a chromatography tank cover arranged on the chromatography device. The temperature control device controls the temperature of the chromatography device arranged in the temperature control device to enable the temperature in the saturating device to rapidly reach equalized temperature required by a test; the structure of the chromatography device can save solvent, is conveniently in presaturation, is convenient to be added with presaturant and developing solvent at any time and is convenient to control the relative humidity in the chromatography device; and the chromatography tank cover is used for sealing the chromatography tank device. the chromatography tank can save the solvent, is convenient in presaturation, is convenient to be added with the presaturant and the developing solvent at any time, is convenient to control the relative humidity and temperature in the saturating device, and has the advantages of low production and use cost, simple operation, easy control of test conditions and favorable chromatography repeated performance, provides reliable test conditions for establishing a thin-layer chromatography fingerprint and is convenient to record the test conditions.
Description
Technical field
Present invention relates in general to the chemical analysis equipment, relate in particular to the thin layer chromatographic cylinder equipment.
Background technology
Conventional thin-layer chromatography is owing to can detect a plurality of samples simultaneously with plate, analysis time is short, fixedly phase (adsorbent) is relatively inexpensive, instant abandoning, needn't worry the pollution of impurity in the sample, detect the interference be not subjected to solvent, the intuitive of chromatogram is strong, can mutually relatively wait advantage and is widely used aspect the chemical analysis with directly perceived observation of plate.Yet affect the many factors of thin-layer chromatographic analysis, cause the repeatability of thin-layer chromatography and comparability poor, be difficult to reach the requirement of international and domestic intercommunication.Wherein with the relevant principal element of chromatography cylinder be:
1, the activity of adsorbent and relative humidity are on the impact of thin-layer chromatography
Because thin layer chromatography is the chromatographic technique of a kind of " open system ", different regions, Various Seasonal cause experimental situation different, and environmental baseline are very large to the chromatography behavioral implications of the separated material of thin-layer chromatography.The activity of adsorbent depends on the relative humidity of experimental situation.In the identical situation of other conditions, relative humidity is clearly on the impact of chromatogram quality.It has been generally acknowledged that the poor reproducibility of thin-layer chromatography, it is one of chief reason that thin layer plate is under the different relative humidity operation.All can reappear test findings in order to reach Various Seasonal between the different experiments chamber, should under the controlled condition of relative humidity, expand into as far as possible suitable.Existing control method has:
1. add the sulfuric acid of debita spissitudo in a side channel of double flute chromatography cylinder, the thin layer plate behind the point sample is put into another side channel, airtight placement 15～30 minutes adds developping agent again and launches in not vitriolated groove.Its weak point is: the interpolation developping agent is inconvenient, when adding developping agent, must open the chromatography cylinder cap, can make like this gas exchange in the interior gas of chromatography cylinder and the external world, destroys the interior various steady state (SS)s of chromatography cylinder; Developping agent is splashed on the thin layer plate, sulfuric acid solution and developping agent are mixed; If requirement of experiment also needs developping agent and other solvents to presaturation in the chromatography cylinder, existing chromatography cylinder does not have the space can deposit other solvents.
2. in preprepared condition control box (shape such as horizontal chromatography cylinder, the sulfuric acid of interior Sheng debita spissitudo, or with the exsiccator of suitable size), carry out.Its weak point is: the space of condition control box is larger, and the experimental implementation process can not be to operate under fully airtight condition, and the experiment condition undulatory property that the condition control box is controlled is larger.
3. select the exsiccator of suitable size, make drying agent with the sulfuric acid of variable concentrations, or use the phosphorus pentoxide vacuum dryer, place thin layer plate on the dividing plate, take out behind the airtight certain hour, at once move in the chromatography cylinder and launch.Its weak point is: the relative humidity in the chromatography cylinder is different with the interior relative humidity of exsiccator, test condition in the chromatography cylinder does not improve, and the thin layer plate that in exsiccator, reaches experiment condition in moving into chromatography cylinder after, the activity of thin layer plate can change along with the environment in the chromatography cylinder, and namely this method controlled humidity does not have effective control to experiment condition.
2, temperature is on the impact of thin-layer chromatography
The impact of temperature is that at first each organic solvent is different because of difference evaporation degree such as boiling point, vapor pressure, evaporation number, relative densities in the developping agent, thereby the vapor ratio of each organic solvent also changes in the developping agent of the space distribution of launching case forms, the inevitable chromatography behavior that directly has influence on material to be separated.Secondly because the variation of temperature, moisture two-dimensional development agent when placing delaminating process or launching in the organic phase ratio of water also different, thereby changed to some extent the polarity of developping agent, the result has influence on the degree of separation of chromatogram.
Existing environmental control system is mainly controlled the temperature in laboratory with equipment such as air-conditionings, and this temperature of indirectly controlling in the chromatography cylinder is difficult to reach the needed temperature conditions of thin-layer chromatography.
3, the steam of solvent is on the impact of thin-layer chromatography
The vapor phase of solvent also participates in development of chromatogram and forms three-dimensional chromatography process in chromatography cylinder.The gas space of chromatography cylinder plays an important role in chromatography process.In regular job, often need to make " chromatography cylinder is saturated ".
Saturated conditions is relevant with the type of chromatography cylinder, conventional chromatography cylinder before expansion difficulty to reach chromatography cylinder saturated.As use the sandwich chromatography cylinder because the space is very little in the case, the space solvent molecule before launching again seldom, and the difficulty diffusion of having living space is so be actually unsaturated.Thin layer plate does not produce pre-adsorption phenomena in this unsaturated sandwich chromatography cylinder.If the space in the chromatography cylinder is too large, before launching or in launching, solvent molecule often forms diffusion gradient, is difficult to for a long time to reach chromatography cylinder saturated, also therefore cause the solvent on the thin layer plate to be easy to volatilization, affected to a great extent the chromatography effect.
In thin-layer chromatographic analysis, adsorb in advance and adsorb and saturated chromatography process is had a great impact.The space of adsorbent surface is very limited, when launching with the polycomponent solvent, the different solvents molecule is vied each other at adsorption zone, occurs " substituting effect ", especially in the bottom of thin layer plate, the sorption isotherm of a component can be subject to the impact of character and the relative saturation degree of other component.Be the water wettability adsorbent such as silica gel, it at first has larger affinity to the polar molecule in the solvent composition, so in the process of adsorption equilibrium, the concentration ratio of each component that is adsorbed is very not identical with the gas space, to form difference larger with the liquid phase of solvent.Can imagine that situation is very complicated in out-of-control situation.In brief, thin layer plate is not when adsorbing in advance, a part of solvent molecule in the mixed solvent system is attracted to selectively on the thin layer plate face and forms fixedly phase, directly affect chromatography process thereby form onboard the solvent gradient simultaneously, the vapor phase of solvent, liquid phase and the fixing three-dimensional chromatography process that has consisted of together mutually a mechanism of action complexity.Existing control method commonly used has:
1. as before being defined in expansion need the solvent pre-equilibration person of chromatography cylinder with developping agent or regulation, can in a side channel of double flute chromatography cylinder, add an amount of solvent, behind the airtight placement certain hour, again thin layer plate is put into chromatography cylinder and launch.Its weak point is: thin layer plate does not participate in pre-equilibration; Must open the chromatography cylinder cap when putting into thin layer plate, the interior gas of chromatography cylinder and the gas in the external world are exchanged, destroy the interior various steady state (SS)s of chromatography cylinder; Without Temperature and Humidity Control; Liquid feeding is inconvenient.
2. need simultaneously pre-equilibration person such as the regulation thin layer plate, then the thin layer plate behind the point sample being placed on the double flute chromatography cylinder does not have in the side channel of solvent, and expansion is front to move developping agent in this groove of people behind the pre-equilibration simultaneously with chromatography cylinder again, launches.Its weak point is: developping agent flows in the side channel that does not have solvent of double flute chromatography cylinder in the bad control chromatography cylinder; Without Temperature and Humidity Control; Liquid feeding is inconvenient.
3. as need to launch in the solvent vapour state of saturation, then add in the inboard of chromatography cylinder one with the filter paper of the inwall same size of cylinder, moistening with developing solvent, behind method presaturation certain hour, the thin layer plate that is loaded with sample is put into rapidly the chromatography expansion cylinder, airtight, launch.Its weak point is: open the chromatography cylinder cap and easily destroy and reach the environment that presaturation requires in the chromatography cylinder; Without Temperature and Humidity Control; Liquid feeding is inconvenient; Thin layer plate has neither part nor lot in presaturation.
4. thin layer plate and chromatography cylinder are simultaneously saturated if needed, then thin layer plate are placed on a side that does not have solvent of double flute chromatography cylinder, and after saturated, expansion cylinder slightly tilted developing solvent flowed in another groove is placed with in the groove of thin layer plate expansion.Its weak point is: without Temperature and Humidity Control; When flowing in the groove developping agent in the groove that is placed with thin layer plate, easily make the position change of thin layer plate, in this simultaneously an end of thin layer plate launch first, launch behind the other end, impact launches effect.
In sum, the major defect of existing chromatography cylinder is:
When (1) adding developping agent and other solvents, must open the chromatography cylinder cap, can make like this gas exchange in the interior gas of chromatography cylinder and the external world, destroy the interior various steady state (SS)s of chromatography cylinder; Developping agent is splashed on the thin layer plate, humidity control agent, presaturation agent and developping agent are mixed.
(2) can not effectively control in real time the interior humidity of chromatography cylinder.
(3) can not effectively control in real time " three-dimensional chromatography " state in the chromatography cylinder in each experimentation.
(5) can not directly monitor the temperature in the chromatography cylinder.
(6) in the practical operation, be easy to destroy the equilibrium state in the chromatography cylinder.
(7) if requirement of experiment should also need developping agent and other solvents to presaturation in the chromatography cylinder by controlled humidity simultaneously, existing chromatography cylinder does not have the space can deposit other solvents.
Existing thin layer chromatographic cylinder mainly is configured to horizontal chromatography cylinder and vertical type chromatography cylinder, and the vertical type chromatography cylinder has single groove chromatography cylinder and double flute chromatography cylinder, because the shortcoming of horizontal chromatography cylinder, vertical type list groove chromatography cylinder is more, now lacked usefulness, vertical type double flute chromatography cylinder uses comparatively general.
Summary of the invention
The object of the invention is to overcome above-mentioned weak point, provide a kind of have save solvent, be convenient to presaturation, be convenient to add at any time solvent, be convenient to control relative humidity in the chromatography cylinder and the chromatographic apparatus utensil of temperature, its production, use cost are low, simple to operate, test condition is easily controlled, the chromatogram Repeatability is good, provide reliable test condition for setting up TLC Fingerprints, be convenient to simultaneously record test condition, further improved the specificity that thin-layer chromatography is differentiated, and reach international and domestic between the requirement of intercommunication.
To achieve the above object, the technical scheme taked of the present invention is: this equipment mainly is made of chromatography cylinder main body, liquid feeding case, humidity control case, presaturation case, temperature-controlled cabinet, chromatography cylinder cap.The Main Function of chromatography cylinder main body is the main container of chromatography process; Liquid feeding case Main Function is to add presaturation agent and developping agent; The humidity control case Main Function is the humidity in the control chromatography cylinder; The temperature of temperature-controlled cabinet control experiment.Two liquid feeding casees have respectively passage to communicate in nearly bottom with chromatography cylinder main body and presaturation case; Gas intercommunication in presaturation case, humidity control case and the chromatography cylinder.
According to the present invention, a kind of chromatography cylinder is provided, comprise temperature control equipment, be contained in chromatographic apparatus and the chromatography cylinder cap that places on the chromatographic apparatus in this temperature control equipment; Wherein, described temperature control equipment control is put in the temperature of chromatographic apparatus wherein, makes the interior temperature of chromatographic apparatus reach fast the equalization temperature of testing requirements; Described chromatographic apparatus is convenient to presaturation and is added presaturation agent and developping agent, is convenient to relative humidity in the key-course analysis apparatus; Described chromatography cylinder cap is used for sealing chromatography cylinder assembly.
According to an aspect of the present invention, described chromatographic apparatus comprises: chromatography cylinder main body 12, as the main container of chromatography process; Presaturation case 16 is placed in the described chromatography cylinder main body of next-door neighbour back, is used for holding the presaturation agent; Humidity control case 10 is for the humidity of controlling by the humidity control agent that is placed in one in chromatography cylinder main body, presaturation case and the humidity control case; Liquid feeding case a 4 communicates in the bottom by passage a 6 and chromatography cylinder main body 12, is used for adding developping agent; Liquid feeding case b 14 communicates in the bottom by passage b 7 and presaturation case 16, is used for adding the presaturation agent; Wherein, humidity control case 10 is arranged on an end of chromatography cylinder main body 12 and presaturation case 16, liquid feeding case a 4, liquid feeding case b 14 are arranged on the other end of chromatography cylinder main body 12 and presaturation case 16, the gas intercommunication in presaturation case 16, humidity control case 10 and the chromatography cylinder main body 12.
Wherein, described chromatography cylinder main body 12, liquid feeding case a 4, liquid feeding case b 14, humidity control case 10 and presaturation case 16 are made by die pressing.
Wherein, described chromatography cylinder main body 12, liquid feeding case a 4, liquid feeding case b 14, humidity control case 10 and presaturation case 16 are made by Method for bonding.
Wherein, described chromatography cylinder main body 12, liquid feeding case a 4, liquid feeding case b 14, humidity control case 10 and presaturation case 16 are made by blowing method.
Wherein, by baffle plate b 11 their the latter half is separated between chromatography cylinder main body 12 and the humidity control case 10, the height of baffle plate b 11 should satisfy the humidity control agent that had both made in the humidity control case 10 and can not flow in the chromatography cylinder main body 12, does not hinder again the Rapid Flow general character of gas.
Wherein, the height of baffle plate b 11 is 1/1000～1 of humidity control case 10 height.
Wherein, by passage c 9 gas between them is communicated between chromatography cylinder main body 12 and the humidity control case 10.
Wherein, by baffle plate d 19 liquid feeding case a 4 and liquid feeding case b 14 are kept apart, the height of baffle plate d 19 is 1/1000～1 of chromatography cylinder main body 12 height.
By baffle plate c 13 liquid feeding case a 4 and liquid feeding case b 14 are separated with chromatography cylinder main body 12, the height of described baffle plate c 13 is to analyse 1/1000～1 of cylinder main body 12 height.
Wherein, liquid feeding case a 4 is connected with the slot segmentation bar 5 of chromatography cylinder main body 12 bottoms by passage b 7, and liquid feeding case b 14 communicates with presaturation case 16 by passage a 6.
Wherein, described slot segmentation bar 5 is the groove shape.
Wherein, by presaturation baffle plate 3 presaturation case 16 and chromatography cylinder main body 12 are separated, the height of presaturation baffle plate 3 is designed to make the presaturation agent can not flow into chromatography cylinder main body 12 to make again chromatography cylinder main body 12 reach fast presaturation simultaneously.
Wherein, by presaturation baffle plate 3 presaturation case 16 and chromatography cylinder main body 12 are separated, described presaturation baffle plate 3 comprises: the first half 17 of mesh; Lower Half 18, it highly is designed to make the presaturation agent can not flow into chromatography cylinder main body 12 to make again chromatography cylinder main body 12 reach fast presaturation simultaneously.
Wherein, the described first half 17 is used for fixedly that presaturation case 16 interior filter paper make it be unlikely to fall in the chromatography cylinder main body 12.
Wherein, the height of described Lower Half 18 is 1/1000～1 of presaturation case 16 height.
Wherein, upper top at baffle plate c 13 has a small front apron a 2, be used for that chromatography cylinder cap 8 prolongs from right to left that chromatography cylinder coboundary 1 is passed and during to liquid feeding case a 4 and liquid feeding case b 14 interior adding solvent, baffle plate a2 matches with chromatography cylinder cap 8 increases in the chromatography cylinder device leakproofness of other containers except liquid feeding case a 4 and liquid feeding case b 14, prevent from destroying saturated in advance three-dimensional chromatography state, the right hand edge that has simultaneously caution chromatography cylinder cap 8 does not surpass the effect of the right hand edge of baffle plate a 2.
Described chromatography cylinder cap 8 lower surfaces, chromatography cylinder coboundary 1 upper surface and baffle plate a 2 upper surfaces are made the frosted shape, and described chromatography cylinder cap 8 is combined closely with baffle plate a 2 and chromatography cylinder coboundary 1, with sealing chromatography cylinder assembly.
Wherein, described temperature control equipment comprises: the heating rod 22 and the gas blender 23 that are positioned at the bottom of temperature-controlled cabinet 15; With the pillar 24 that the bottom of temperature-controlled cabinet 15 is connected, support described chromatographic apparatus and make it not and heating rod 22 and gas blender 23 joints; Be positioned at the temperature inductor 20 of the first half of temperature-controlled cabinet 15; Be positioned at the digital control system 21 of temperature-controlled cabinet side, be used for setting and the control temperature.
According to another aspect of the present invention, described chromatographic apparatus comprises: chromatography cylinder main body 12, as the main container of chromatography process; Presaturation case a places in the described chromatography cylinder main body of next-door neighbour back, is used for holding the presaturation agent; Presaturation case b places in the described chromatography cylinder main body of next-door neighbour front, is used for holding other presaturation agent; Humidity control case 10 is for the humidity of controlling by the humidity control agent that is placed in one in chromatography cylinder main body, presaturation case and the humidity control case; Liquid feeding case a 4 communicates in the bottom by passage a 6 and chromatography cylinder main body 12, is used for adding developping agent; Liquid feeding case b 14 communicates in the bottom with presaturation case a by passage b 7, is used for adding the presaturation agent; Liquid feeding case c communicates in the bottom with presaturation case b by passage c, is used for adding other presaturation agent; Wherein, humidity control case 10 is arranged on the end of chromatography cylinder main body 12 and presaturation case a, presaturation case b, liquid feeding case a 4, liquid feeding case b 14, liquid feeding case c are arranged on the other end of chromatography cylinder main body 12 and presaturation case a, presaturation case b, the gas intercommunication in presaturation case a, presaturation case b, humidity control case 10 and the chromatography cylinder main body 12.
Advantage of the present invention is:
1, save solvent, be convenient to presaturation, be convenient to add at any time presaturation agent and developping agent, be convenient to control relative humidity and temperature in the expansion cylinder.
2, its production, use cost are low, simple to operate, test condition is easily controlled, the chromatogram Repeatability is good, provide reliable test condition for setting up TLC Fingerprints, be convenient to simultaneously record test condition, further improved the specificity that thin-layer chromatography is differentiated, and reach international and domestic between the requirement of intercommunication.
Description of drawings
Fig. 1 is structural representation of the present invention;
Fig. 2 is the cut-away view of the A-B of Fig. 1;
Fig. 3 is the schematic diagram of the presaturation baffle plate 3 that is connected with the chromatography cylinder main body of Fig. 1 presaturation case;
Fig. 4 is the cut-away view of liquid feeding case;
Fig. 5 is the schematic diagram of temperature-controlled cabinet;
Fig. 6 is the schematic diagram of chromatography cylinder cap.
Wherein:
1 chromatography cylinder coboundary
One baffle plate of 2 liquid feeding casees and chromatography cylinder main body junction (being called for short baffle plate a 2)
3 presaturation baffle plates
4 developping agent liquid feeding casees (referred to as liquid feeding case a 4)
Slot segmentation bar in the 5 expansion cylinder main bodys (being called for short slot segmentation bar 5)
The passage (referred to as passage a 6) that 6 liquid feeding case a 4 are connected with the presaturation case
The passage that 7 liquid feeding case b 14 are connected with the chromatography cylinder main body (being called for short passage b 7)
8 chromatography cylinder caps
The part that 9 humidity control cases communicate with the chromatography cylinder main body (being called for short passage c 9)
10 humidity control cases
The baffle plate that 11 humidity control cases and chromatography cylinder main body are cut apart mutually (being called for short baffle plate b 11)
12 chromatography cylinder main bodys
Baffle plate between 13 liquid feeding casees and the chromatography cylinder main body (being called for short baffle plate c 13)
14 presaturation agent liquid feeding casees (being called for short liquid feeding case b 14)
15 temperature-controlled cabinets
16 presaturation casees
The first half of 17 presaturation case baffle plates
The latter half of 18 presaturation case baffle plates
Baffle plate (referred to as baffle plate d 19) between 19 two liquid feeding casees
20 temperature inductors
21 digital control system
22 heating rods
23 gas blender
24 pillars
Below in conjunction with accompanying drawing the embodiment of the invention is described in further detail.
Embodiment
Referring to Fig. 1, constant temperature and humidity thin-layer chromatography tank of the present invention comprises chromatography cylinder main body 12, liquid feeding case a 4, liquid feeding case b 14, humidity control case 10, presaturation case 16, temperature-controlled cabinet 15, chromatography cylinder cap 8 at least.Chromatography cylinder main body 12, liquid feeding case a 4, liquid feeding case b 14, humidity control case 10, presaturation case 16 are by die pressing or Method for bonding or blowing method be made (referred to as chromatographic apparatus).Humidity control case 10 and liquid feeding case a 4, liquid feeding case b 14 is staggered relatively at the two ends of chromatography cylinder main body 12.In a preferred embodiment of the invention, humidity control cases 10 on the left side of chromatography cylinder main body 12, by baffle plate b 11 their the latter half being separated between chromatography cylinder main body 12 and presaturation case 16 and the humidity control case 10, mainly is that the humidity control agent in the isolation humidity control case 10 flows in chromatography cylinder main body 12 and the presaturation case 16; The height of baffle plate b 11 should satisfy the humidity control agent that both must not make in the humidity control case 10 and flow in chromatography cylinder main body 12 and the presaturation case 16 the Rapid Flow general character that can not hinder again gas.Preferably, the height of baffle plate b 11 is 1/1000～1 of humidity control case 10 height; By passage c 9 gas between them is communicated between chromatography cylinder main body 12, presaturation case 16 and the humidity control case 10.Passage c 9 is that chromatography cylinder coboundary 1 surface level is apart from the space of baffle plate 11 coboundarys.The humidity control agent that fills in the humidity control case 10 utilizes the humidity in principle control chromatography cylinder main body 12, humidity control case 10 and the presaturation case 16 of the diffusion of the volatilization of liquid and gas, the mobile equilibrium between absorbing, and guarantees that its humidity reaches the condition of experiment.Humidity control agent should be suitable mutually with requirement of experiment, and humidity control agent can be that solid (phosphorus pentoxide etc.) also can be liquid (sulfuric acid solution etc.).Liquid feeding case a 4, liquid feeding case b 14 separate by baffle plate d 19 between liquid feeding case a 4 and the liquid feeding case b 14 mutually on the right of chromatography cylinder main body 12, make it not intercommunication.Preferably, the height of baffle plate d 19 is 1/1000～1 of chromatography cylinder main body 12 height, and its effect is that two kinds of liquid spatter mutually when avoiding liquid feeding, and presaturation liquid and developping agent are mixed mutually to cause the failure of testing and make.Liquid feeding case a 4 is connected with the slot segmentation bar 5 of chromatography cylinder main body 12 by passage b 7, and is convenient at any time to chromatography cylinder main body 12 interior interpolation developping agents; The bottom separation trough bar 5 of chromatography cylinder main body is the groove shape
The major advantage of the dividing strip of groove shape is: have the chromatography cylinder bottom of groove shape dividing strip than tabular (-) or tongue shape
Chromatography cylinder bottom save developping agent, and can play the effect of anti-its slip of fixed film plate, when adding the developping agent expansion, the developping agent liquid level in the slot segmentation bar launches increasing of dosage along with adding and rises, be unlikely to make an end of thin layer plate to launch first, launch behind the other end.Liquid feeding case b 14 communicates with presaturation case 16 by passage a 6, and is convenient at any time to the 16 interior interpolation presaturation agent of presaturation case.Liquid feeding case a 4 and liquid feeding case b 14 make it to separate with chromatography cylinder main body 12 and presaturation case 16 by baffle plate c 13; Preferably, the height of baffle plate c 13 is to analyse 1/1000～1 of cylinder main body 12 height.Chromatography cylinder cap 8 is a flat board, and its size and shape should be selected according to the size and shape of chromatographic apparatus, but complete sealant analysis apparatus; One fritter baffle plate a 2 is arranged on the upper top of baffle plate c 13, baffle plate a 2 is positioned at same level with chromatography cylinder coboundary 1, the effect of baffle plate a 2 is when chromatography cylinder cap 8 is passed and during to liquid feeding case a 4 and liquid feeding case b 14 interior adding solvent along chromatography cylinder coboundary 1 from right to left, baffle plate a 2 matches with chromatography cylinder cap 8 increases in the chromatography cylinder device leakproofness of other containers except liquid feeding case a 4 and liquid feeding case b 14, prevent from destroying in advance saturated " three-dimensional chromatography " state, the right hand edge that has simultaneously caution chromatography cylinder cap 8 does not surpass the effect (being mark action) of the right hand edge of baffle plate a 2.Wherein chromatography cylinder cap 8 lower surfaces, chromatography cylinder coboundary 1 upper surface and baffle plate a 2 upper surfaces are made the frosted shape.Chromatography cylinder cap 8 is combined closely with baffle plate a 2 and chromatography cylinder coboundary 1, its effect is to chromatography cylinder main body 12, liquid feeding case a 4, liquid feeding case b 14, humidity control case 10,16 sealings of presaturation case, prevent in chromatography cylinder main body 12, liquid feeding case a 4, liquid feeding case b 14, humidity control case 10, the presaturation case 16 gas wave loose, the intercommunication of gas in the air that isolation is extraneous and the case.Chromatography cylinder coboundary 1 is chromatographic apparatus edge suitable for reading, in order to increase the leakproofness of chromatographic apparatus, chromatography cylinder coboundary 1 can be made outwards extended with respect to chromatographic apparatus coboundary surface level, the length Ying Yineng that wherein outwards extends guarantees that the leakproofness of chromatographic apparatus is advisable, and should be 1/1000～1 of chromatography cylinder body height.Presaturation case 16 is positioned at the back of chromatography cylinder main body 12, by presaturation baffle plate 3 its latter half is separated between presaturation case 16 and the chromatography cylinder main body 12, prevents that the presaturation agent from flowing into chromatography cylinder main body 12.The first half 17 of presaturation baffle plate 3 is mesh, its effect is to make gas intercommunication between presaturation case 16 and the chromatography cylinder main body 12, and play filter paper in the fixing presaturation case 16 and make it be unlikely to fall into effect in the chromatography cylinder main body 12, or remove 17, convenient production.After removing 17, presaturation baffle plate 3 is the latter half 18 of presaturation baffle plate 3.The height of the latter half 18 of presaturation baffle plate 3 can not be too high or too low, too high then can not reaching makes chromatography cylinder main body 12 quick pre-saturated effects, cross and low the presaturation agent is flowed in the chromatography cylinder main body 12, the presaturation dosage that causes simultaneously adding is very few and can not reach pre-saturated effect.Preferably, it highly is 1/1000～1 of presaturation case 16 height.
Temperature control equipment 15 is that control is put in the temperature of chromatographic apparatus wherein, thereby makes the interior temperature of chromatographic apparatus reach fast the equalization temperature of testing requirements.Temperature control equipment 15 can be a container type, also can be other shapes, but preferably should be chromatographic apparatus can be put in the inner, by the temperature in gas or the liquid media key-course analysis apparatus; If adopt the complete structure placed on it of chromatographic apparatus, can make the inequality of being heated of chromatography cylinder, bottom temp is high, and it is long that whole chromatographic apparatus reaches the needed time of homogenization temperature, even do not reach homogenization temperature, the effect of impact experiment.Pillar 24 is connected with temperature-controlled cabinet 10 bottoms, and its height is with can the supporting layer analysis apparatus and do not contact heating rod 22 and gas blender 23, and the quick control that does not affect 22 pairs of chromatographic apparatus temperature homogeneities of heating rod is as the criterion.The shape of pillar 24 can be cylindrical or strip or other shape or a dividing plate with holes.Heating rod 22 and gas blender 23 are positioned at the bottom of temperature-controlled cabinet 15, make the temperature homogeneous in the temperature-controlled cabinet 15.Temperature inductor 20 is positioned at the first half of temperature-controlled cabinet 15.Digital control system 21 take convenient set, the control temperature as principle can be positioned at temperature-controlled cabinet before, upper, left and right.
The material of chromatographic apparatus, chromatography cylinder cap 8 usefulness is transparent material among the present invention, and miscellaneous part is selected economic suitable material.
Liquid feeding case of the present invention, presaturation case, humidity control case and chromatography cylinder main body can be square, circular or other shapes.
Liquid feeding case a 4 among the present invention is connected and can not be connected mutually with liquid feeding case b, is respectively a container with cover independently.Liquid feeding case a 4 links to each other with presaturation case 16 nearly bottoms by passage a 6, and its link position is looked conveniently and produced, makes things convenient for experimental implementation to be as the criterion; Liquid feeding case b 14 links to each other with chromatography cylinder main body 12 by passage b 7, and its link position is looked conveniently and produced, makes things convenient for experimental implementation to be as the criterion.Acting as of the chromatography cylinder cap of this moment seals chromatography cylinder main body 12, presaturation case 16, humidity control case 10.
According to another embodiment of the invention, can be according to the requirement of test condition, respectively there is respectively a presaturation case front and back in the chromatography cylinder main body, the presaturation case is divided into developping agent presaturation case a and other solvent presaturation casees b, the liquid feeding case extends to three, be respectively liquid feeding case a, liquid feeding case b and liquid feeding case c, its connected mode is with above-mentioned connected mode.The effect of liquid feeding case a and liquid feeding case b as hereinbefore, liquid feeding case c be used for to add other solvents, satisfies the requirement of using simultaneously developping agent and other solvent presaturation chromatography cylinder main bodys.Other solvent presaturation casees b communicates in the bottom with presaturation case b by passage c, is used for adding other presaturation agent.Other structures of chromatographic apparatus are identical with previous embodiment of the present invention.
The used temperature conduction medium of temperature control equipment of the present invention can be gas, also can be liquid.If take gas as the temperature conduction medium then can carry out the control of omnibearing temperature to experimental apparatus.Schematic diagram of the present invention is take liquid as the temperature conduction medium experimental apparatus to be carried out temperature control, and its advantage has production, use cost low, and laboratory operation is simple, can reach the requirement of experiment condition simultaneously.
The size of chromatography cylinder main body of the present invention should be slightly larger than thin layer plate, conveniently puts into and take out thin layer plate, and do not affect in the chromatographic apparatus between all parts gas mutually fast circulation, exchange be as the criterion, namely be advisable not affect presaturation effect and humidity control effect.
The size of presaturation case of the present invention should satisfy the quick presaturation effect of chromatographic apparatus and be advisable.
The size of liquid feeding case of the present invention should conveniently be as the criterion with liquid feeding.
The size of temperature-controlled cabinet of the present invention should be suitable mutually with chromatographic apparatus.
The size of humidity control case of the present invention should be able to satisfy and reaches fast humidity control effect and be advisable.
The aperture of the first half 17 of presaturation baffle plate of the present invention can be circular or other shapes.
Passage a 6 of the present invention and passage b 7 can be circular or other shapes.
0～100 ℃ of the temperature-control range of temperature-controlled cabinet of the present invention.
The relative humidity range of control of humidity control case of the present invention is 0～100%.
Principle of work:
Utilize the diffusion of the volatilization of liquid and gas to make humidity control agent control chromatography cylinder main body in the humidity control case, the in advance humidity of full case, thin layer plate, and the saturated chromatography cylinder main body of presaturation agent, in advance full case, thin layer plate.
Do medium with gas or liquid, by the temperature in the accurate control of the temperature control equipment chromatography cylinder.
Claims (8)
1. a chromatography cylinder comprises temperature control equipment, is contained in chromatographic apparatus and the chromatography cylinder cap that places on the chromatographic apparatus in this temperature control equipment; Wherein, described temperature control equipment control is put in the temperature of chromatographic apparatus wherein, makes the interior temperature of chromatographic apparatus reach fast the equalization temperature of testing requirements; Described chromatographic apparatus is convenient to presaturation and is added presaturation agent and developping agent, is convenient to relative humidity in the key-course analysis apparatus; Described chromatography cylinder cap is used for sealing chromatography cylinder assembly;
Described chromatographic apparatus comprises:
Chromatography cylinder main body (12) is as the main container of chromatography process;
Presaturation case (16) is placed in the described chromatography cylinder main body of next-door neighbour back, is used for holding the presaturation agent;
Humidity control case (10) is for the humidity of controlling by the humidity control agent that is placed in one in chromatography cylinder main body, presaturation case and the humidity control case;
Liquid feeding case a (4) communicates in the bottom with chromatography cylinder main body (12) by passage a (6), is used for adding developping agent;
Liquid feeding case b (14) communicates in the bottom with presaturation case (16) by passage b (7), is used for adding the presaturation agent;
Wherein, humidity control case (10) is arranged on an end of chromatography cylinder main body (12) and presaturation case (16), liquid feeding case a (4) and liquid feeding case b (14) are arranged on the other end of chromatography cylinder main body (12) and presaturation case (16), the gas intercommunication in presaturation case (16), humidity control case (10) and the chromatography cylinder main body (12);
Described temperature control equipment comprises:
Be positioned at heating rod (22) and the gas blender (23) of the bottom of temperature-controlled cabinet (15);
With the pillar (24) that the bottom of temperature-controlled cabinet (15) is connected, support described chromatographic apparatus and make it not contact with heating rod (22) and gas blender (23);
Be positioned at the temperature inductor (20) of the first half of temperature-controlled cabinet (15); With the digital control system that is positioned at the temperature-controlled cabinet side (21), be used for setting and the control temperature.
2. described chromatography cylinder as claimed in claim 1, wherein, described chromatography cylinder main body (12), liquid feeding case a (4), liquid feeding case b (14), humidity control case (10) and presaturation case (16) are made by die pressing or Method for bonding or blowing method.
3. described chromatography cylinder as claimed in claim 1, wherein, by baffle plate b (11) their the latter half is separated between chromatography cylinder main body (12) and the humidity control case (10), the height of baffle plate b (11) should satisfy the humidity control agent that had both made in the humidity control case (10) and can not flow in the chromatography cylinder main body (12), does not hinder again the Rapid Flow general character of gas;
By passage c (9) gas between them is communicated between chromatography cylinder main body (12) and the humidity control case (10),
Liquid feeding case a (4) is connected with the slot segmentation bar (5) of chromatography cylinder main body (12) bottom by passage a (6), and liquid feeding case b (14) communicates with presaturation case (16) by passage b (7);
By baffle plate d (19) liquid feeding case a (4) and liquid feeding case b (14) are kept apart, the height of baffle plate d (19) is 1/1000～1 of chromatography cylinder main body (12) height;
By baffle plate c (13) liquid feeding case a (4) and liquid feeding case b (14) are separated with chromatography cylinder main body (12), the height of described baffle plate c (13) is 1/1000～1 of chromatography cylinder main body (12) height;
By presaturation baffle plate (3) presaturation case (16) and chromatography cylinder main body (12) are separated, the height of presaturation baffle plate (3) is designed to make the presaturation agent can not flow into chromatography cylinder main body (12) simultaneously to make again chromatography cylinder main body (12) reach fast presaturation.
4. described chromatography cylinder as claimed in claim 3, wherein, the height of baffle plate b (11) is 1/1000～1 of humidity control case (10) height.
5. described chromatography cylinder as claimed in claim 4, wherein, described slot segmentation bar (5) is the groove shape.
6. described chromatography cylinder as claimed in claim 1 wherein, is separated presaturation case (16) and chromatography cylinder main body (12) by presaturation baffle plate (3), and described presaturation baffle plate (3) comprising:
The first half of mesh (17), being used for fixedly, the interior filter paper of presaturation case (16) makes it be unlikely to fall in the chromatography cylinder main body (12); And Lower Half (18), it highly is designed to 1/1000～1 of presaturation case (16) height, makes again chromatography cylinder main body (12) reach fast presaturation simultaneously so that the presaturation agent can not flow into chromatography cylinder main body (12).
7. described chromatography cylinder as claimed in claim 3, wherein, upper top at baffle plate c (13) has a small front apron a (2), be used for that chromatography cylinder cap (8) prolongs from right to left that chromatography cylinder coboundary (1) is passed and in liquid feeding case a (4) and the liquid feeding case b (14) during the adding solvent, baffle plate a (2) matches with chromatography cylinder cap (8) increases in the chromatography cylinder device leakproofness of other containers except liquid feeding case a (4) and liquid feeding case b (14), prevent from destroying saturated in advance three-dimensional chromatography state, the right hand edge that has simultaneously caution chromatography cylinder cap (8) does not surpass the effect of the right hand edge of baffle plate a (2);
Described chromatography cylinder cap (8) lower surface, chromatography cylinder coboundary (1) upper surface and baffle plate a (2) upper surface are made the frosted shape, described chromatography cylinder cap (8) is combined closely with baffle plate a (2) and chromatography cylinder coboundary (1), with sealing chromatography cylinder assembly.
8. a chromatography cylinder comprises temperature control equipment, is contained in chromatographic apparatus and the chromatography cylinder cap that places on the chromatographic apparatus in this temperature control equipment; Wherein, described temperature control equipment control is put in the temperature of chromatographic apparatus wherein, makes the interior temperature of chromatographic apparatus reach fast the equalization temperature of testing requirements; Described chromatographic apparatus is convenient to presaturation and is added presaturation agent and developping agent, is convenient to relative humidity in the key-course analysis apparatus; Described chromatography cylinder cap is used for sealing chromatography cylinder assembly;
Wherein, described chromatographic apparatus comprises:
Chromatography cylinder main body (12) is as the main container of chromatography process;
Presaturation case a places in the described chromatography cylinder main body of next-door neighbour back, is used for holding the presaturation agent;
Presaturation case b places in the described chromatography cylinder main body of next-door neighbour front, is used for holding other presaturation agent;
Humidity control case (10) is for the humidity of controlling by the humidity control agent that is placed in one in chromatography cylinder main body, presaturation case a, presaturation case b and the humidity control case;
Liquid feeding case a (4) communicates in the bottom with chromatography cylinder main body (12) by passage a (6), is used for adding developping agent;
Liquid feeding case b (14) communicates in the bottom with presaturation case a by passage b (7), is used for adding the presaturation agent;
Liquid feeding case c communicates in the bottom with presaturation case b by passage, is used for adding other presaturation agent;
Wherein, humidity control case (10) is arranged on the end of chromatography cylinder main body (12) and presaturation case a, presaturation case b, liquid feeding case a (4), liquid feeding case b (14), liquid feeding case c are arranged on the other end of chromatography cylinder main body (12) and presaturation case a, presaturation case b, the gas intercommunication in presaturation case a, presaturation case b, humidity control case (10) and the chromatography cylinder main body (12);
Described temperature control equipment comprises:
Be positioned at heating rod (22) and the gas blender (23) of the bottom of temperature-controlled cabinet (15);
With the pillar (24) that the bottom of temperature-controlled cabinet (15) is connected, support described chromatographic apparatus and make it not contact with heating rod (22) and gas blender (23);
Be positioned at the temperature inductor (20) of the first half of temperature-controlled cabinet (15); With the digital control system that is positioned at the temperature-controlled cabinet side (21), be used for setting and the control temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910081467 CN101858900B (en) | 2009-04-08 | 2009-04-08 | Constant temperature and humidity thin-layer chromatography tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910081467 CN101858900B (en) | 2009-04-08 | 2009-04-08 | Constant temperature and humidity thin-layer chromatography tank |
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| Publication Number | Publication Date |
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| CN101858900A CN101858900A (en) | 2010-10-13 |
| CN101858900B true CN101858900B (en) | 2013-01-16 |
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| CN 200910081467 Expired - Fee Related CN101858900B (en) | 2009-04-08 | 2009-04-08 | Constant temperature and humidity thin-layer chromatography tank |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106290696B (en) * | 2015-06-23 | 2023-04-11 | 中美华世通生物医药科技(武汉)股份有限公司 | Thin-layer chromatography system and chromatography method |
| CH715116A1 (en) * | 2018-06-21 | 2019-12-30 | Camag Chemie Erzeugnisse Und Adsorptionstechnik Ag | Method and device for the automatic chromatography of thin-layer plates. |
| CN109061030B (en) * | 2018-09-20 | 2020-09-15 | 广西壮族自治区药用植物园 | Quality detection method of antitoxic leukogenic mixture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6096205A (en) * | 1998-05-13 | 2000-08-01 | The Regents Of The University Of California | Hand portable thin-layer chromatography system |
| CN2781369Y (en) * | 2005-04-26 | 2006-05-17 | 刘吉成 | Thin-layer chromatography program temp. control instrument |
| CN101059488A (en) * | 2006-10-27 | 2007-10-24 | 张兰月 | Chromatography tank for implementing minimum dosage of thin layer chromatographic developing solvent |
| CN201464442U (en) * | 2009-04-08 | 2010-05-12 | 马启武 | Constant temperature and humidity thin-layer chromatography cylinder |
-
2009
- 2009-04-08 CN CN 200910081467 patent/CN101858900B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6096205A (en) * | 1998-05-13 | 2000-08-01 | The Regents Of The University Of California | Hand portable thin-layer chromatography system |
| CN2781369Y (en) * | 2005-04-26 | 2006-05-17 | 刘吉成 | Thin-layer chromatography program temp. control instrument |
| CN101059488A (en) * | 2006-10-27 | 2007-10-24 | 张兰月 | Chromatography tank for implementing minimum dosage of thin layer chromatographic developing solvent |
| CN201464442U (en) * | 2009-04-08 | 2010-05-12 | 马启武 | Constant temperature and humidity thin-layer chromatography cylinder |
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| CN101858900A (en) | 2010-10-13 |
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