CN101858862A - Method and system for quickly detecting thioglycoside content of rape seeds - Google Patents
Method and system for quickly detecting thioglycoside content of rape seeds Download PDFInfo
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- CN101858862A CN101858862A CN 201010175823 CN201010175823A CN101858862A CN 101858862 A CN101858862 A CN 101858862A CN 201010175823 CN201010175823 CN 201010175823 CN 201010175823 A CN201010175823 A CN 201010175823A CN 101858862 A CN101858862 A CN 101858862A
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- Prior art keywords
- rapeseed
- content
- reflectivity
- rape seeds
- wavelength
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- 240000002791 Brassica napus Species 0.000 title claims abstract description 21
- 235000011293 Brassica napus Nutrition 0.000 title claims abstract description 20
- 229930182475 S-glycoside Natural products 0.000 title claims abstract description 18
- 150000003569 thioglycosides Chemical class 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000012417 linear regression Methods 0.000 claims abstract description 9
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 38
- 244000188595 Brassica sinapistrum Species 0.000 claims description 37
- 238000002310 reflectometry Methods 0.000 claims description 20
- 238000001228 spectrum Methods 0.000 claims description 12
- 125000004383 glucosinolate group Chemical group 0.000 claims description 11
- 238000012545 processing Methods 0.000 claims description 11
- 239000005864 Sulphur Substances 0.000 claims description 9
- 229930182478 glucoside Natural products 0.000 claims description 9
- -1 sulphur glucoside Chemical class 0.000 claims description 9
- 238000006467 substitution reaction Methods 0.000 claims description 5
- 238000001514 detection method Methods 0.000 abstract description 10
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 238000004364 calculation method Methods 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 5
- 235000019198 oils Nutrition 0.000 description 5
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 description 4
- URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 description 4
- DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 208000035126 Facies Diseases 0.000 description 1
- 235000019484 Rapeseed oil Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a method for quickly detecting thioglycoside content of rape seeds, which comprises the following steps of: 1) acquiring reflection rates of the rape seeds corresponding to light waves with wavelengths of 672 nanometers, 493 nanometers, 841 nanometers and 423 nanometers respectively; and 2) substituting the reflection rates into a multiplex linear regression equation y = 4619lambda1 - 6593lambda2 - 771.077lambda3 + 1798lambda4 + 153.084, and obtaining the thioglycoside content of the rape seeds through calculation, wherein in the equation, y is the thioglycoside content of the rape seeds, and lambda1 to lambda4 are the reflection rates of the rape seeds corresponding to the light waves with the wavelengths of 672 nanometers, 493 nanometers, 841 nanometers and 423 nanometers respectively. The method is mainly used for quickly and accurately detecting the thioglycoside content of the rape seeds through characteristic wavelengths, has quick and accurate detection, greatly shortens the detection time, reduces the environmental pollution and lowers the detection cost.
Description
Technical field
The present invention relates to the rape detection range, relate in particular to a kind of thioglycoside content of rape seeds method for quick and system.
Background technology
Rape is one of the main oil crops of China and nectar source crop, and rapeseed is one of system immersion oil fat raw material principal item.The cultivated area of rape accounts for more than 40% of the national oil crops total area, and output accounts for more than 60% of national edible vegetable oil total production, occupies first place in the world.The quick evaluation of rapeseed quality index and detection are to rapeseed cultivation and produce significant.The content of sulphur glucoside, erucic acid is the important indicator of estimating the rapeseed quality quality in the rapeseed.Quality rape is the double-low rapeseed of low sulfatide, low erucic acid, and the rapeseed oil content of erucic acid that is pressed by double-low rapeseed is low, is of high nutritive value, and is of value to health.Thus, erucic acid, glucosinolate content in the rapeseed are carried out fast, accurately measure, two low quality rapes and conventional oil vegetable-growing area branch can be come, implementation is purchase separately, processing separately, the excellent usefulness of high-quality of realization double-low rapeseed.
After Chinese patent application 01133507.6 discloses and has got the pulverizing of 1～5g vegetable seed sample, take by weighing 0.20～0.80g and place tool plug test tube, add 1.0～5.0ml distilled water, shake up, sulphur glucoside degradation reaction takes place, filter behind the stoichiometric number minute, get 20～80 μ l filtrates and place sulphur glucoside test board, after adding potassium dihydrogen phosphate aqueous solution 60～240 μ l stoichiometric numbers minute, form coloured product, because the solution colour depth is relevant with glucosinolate content in the sample, adopts spectrophotometric method and can measure glucosinolate content with measuring instrument according to typical curve.This method need be handled rapeseed before detection, complicated operation.
As seen/near infrared spectrum can reflect that the frequency multiplication of groups such as the organic principle of material inside, particularly C-H, O-H, N-H and sum of fundamental frequencies absorb, and can be used for the content of quantitative measurement material organic substance.Spectral technique has been widely used in industries such as agricultural, food, petrochemical complex, pharmacy, feed.
Summary of the invention
The invention provides a kind of thioglycoside content of rape seeds method for quick, solved existing detection method and needed to the rapeseed sample pretreatment problem of complicated operation.
A kind of thioglycoside content of rape seeds method for quick may further comprise the steps:
1) reflectivity of collection wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave;
2) with each reflectivity difference substitution multiple linear regression equations: y=4619 λ
1-6593 λ
2-771.077 λ
3+ 1798 λ
4+ 153.084, calculate the glucosinolate content of rapeseed, sulphur glucoside unit is μ mol/g;
Y is the content of sulphur glucoside in the rapeseed in the equation; λ
1～λ
4Be respectively the reflectivity of wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave.
Because rapeseed is a non-transparent medium, when light-wave irradiation in its when surface, except that part is absorbed, all the other all reflect, and what (perhaps reflectivity) that light wave absorbs are to be correlated with the inner content of chemical substances of rapeseed, and only follow the reflectivity of some characteristic wavelength light wave of minority relevant at the content of the concrete material of this class of sulphur glucoside.Use the successive projection algorithm and in 350～1100nm wavelength coverage, carry out choosing of characteristic wavelength, obtain these characteristic wavelengths and be respectively 672nm, 493nm, 841nm, 423nm.
The present invention also provides a kind of system of fast detecting thioglycoside content of rape seeds, comprising:
Sample cell: be used for the splendid attire rapeseed, the bottom surface is transparent optics window;
The spectra collection device: be located at the optics window under, be used to gather the reflectivity of wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave;
Processing module: receive each reflectivity and substitution equation:: y=4619 λ
1-6593 λ
2-771.077 λ
3+ 1798 λ
4+ 153.084, calculate glucosinolate content in the rapeseed;
Y is the content of sulphur glucoside in the rapeseed in the equation; λ
1～λ
4Be respectively the reflectivity of wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave.
Preferably, described spectra collection device is by being in the light source in the same plane and forming with 4 photosensitive components, and the center of light source and optics window is on the same vertical straight line; All photosensitive components are that the center evenly distributes with the light source, and the plane at their places is parallel with the optics window, and this is simple in structure, and is easily manufactured.
Described photosensitive components preferably is made up of the filter plate at top and the light sensor of bottom, and is simple in structure, easily manufactured.
Preferably, said system comprises and is used for supply unit that spectra collection device and processing module are powered, is easy to carry.
Preferably, described sample cell is provided with handle, is convenient to manipulate with integrated.
Described spectra collection device preferably is connected with processing module by the USB interface data line of being located at handle inside, and the profile outward appearance is easy to assembly.
The beneficial effect that the present invention has is:
(1) realizes the detection quick and precisely of glucosinolate content in the rapeseed, had real-time, quick, easy characteristics;
(2) adopt a few features wavelength to carry out multiple linear regression analysis, detect fast, antijamming capability is strong;
(3) this pick-up unit adopts contactless analytical technology, need be through processes such as chemical treatments, and testing process does not produce any pollution, and the measured oil vegetable seed is not had any infringement and influence.
The present invention is mainly used in by characteristic wavelength and quick and precisely detects thioglycoside content of rape seeds, detects quick and precisely, has shortened the time of detecting greatly, has reduced environmental pollution, has reduced the detection cost.
Description of drawings
Fig. 1 is the structural representation of system of the present invention;
Fig. 2 is the vertical view of system shown in Figure 1;
Fig. 3 is the structural representation of photosensitive components in the system shown in Figure 1.
Embodiment
As shown in the figure, a kind of system of fast detecting thioglycoside content of rape seeds comprises sample cell 1, spectra collection device 2 and processing module 3, and wherein the bottom surface of sample cell 1 is transparent optics window 11, guarantees its not extinction, does not influence reflectivity and detects.
Also be provided with handle 4 on the sample cell 1, easy to use, spectra collection device 2 is connected with processing module 3 by the USB interface data line in the handle 4 simultaneously.Processing module 3 is fixed on the handle 4.It is core with the single-chip microcomputer, also comprises storer, display etc.In order to reach the purpose of portable use, the also fixing supply unit of being made up of accumulator and voltage stabilizer on handle 4 is spectra collection device 2 and processing module power supply.
Spectra collection device 2 be positioned at optics window 11 under, it is by being in conplane light source 21 and 4 photosensitive components 22 are formed, light source can send the constant continuous light of light intensity of wavelength 350～1100nm scope, and the center of it and optics window 11 is on the same vertical straight line.Photosensitive components 22 is made up of the filter plate 221 of top layer and the light sensor 222 of bottom, and all photosensitive components 22 are that the center evenly distributes with light source 11.
The present invention measures the reflectance value of 225 parts of rapeseed samples at wavelength 672nm, 493nm, 841nm, 423nm place earlier, select at random then 150 increments this as modeling collection sample, all the other 75 parts as the forecast set sample, utilize the glucosinolate content of 225 parts of rapeseed of traditional technique in measuring simultaneously, by multiple linear regression analysis method (Multiple linear regression MLR) obtains following multiple linear regression equations:
y＝4619λ
1-6593λ
2-771.077λ
3+1798λ
4+153.084
Y is the content of sulphur glucoside in the rapeseed in the journey; λ
1～λ
4Be respectively the reflectivity of wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave.
Utilize the glucosinolate content of above-mentioned multiple linear regression equations forecast modeling collection sample and forecast set sample then, and estimate predicting the outcome.Related coefficient and slope approach 1 more in the evaluation index, and the absolute value of root-mean-square error, deviation and the absolute value of intercept are more little, illustrate that the model prediction performance is good more.It is as shown in the table to predict the outcome:
| Related coefficient | Root-mean-square error | Deviation | Slope | Intercept | |
| Modeling | ??0.9666 | ??5.3472 | ??-2.2×10 -5 | ??0.9343 | ??2.3368 |
| Prediction | ??0.9540 | ??6.3529 | ??-0.5589 | ??0.9035 | ??2.9215 |
Modeling collection and forecast set are predicted, facies relationship number average between the predicted value of thioglycoside content of rape seeds and the true chemical method measured value is greater than 0.95, obtained satisfied precision of prediction, illustrated and use the detection that method of the present invention can realize thioglycoside content of rape seeds rapidly and accurately.
It is as follows that said apparatus detects the thioglycoside content of rape seeds method:
Rapeseed is positioned in the sample cell 1, open light source 21 vertical irradiation optics windows 11 then, light sensor 222 receives only the reflected light of specific wavelength by filter plate 221, light sensor 221 converts according to the original light intensity of light source 21 and catoptrical light intensity and obtains the light wave reflectivity of characteristic wavelength, single-chip microcomputer receives these reflectivity values by data line, in the substitution equation of linear regression, calculate thioglycoside content of rape seeds.
Claims (7)
1. thioglycoside content of rape seeds method for quick may further comprise the steps:
1) reflectivity of collection wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave;
2) with each reflectivity difference substitution multiple linear regression equations: y=4619 λ
1-6593 λ
2-771.077 λ
3+ 1798 λ
4+ 153.084, calculate the glucosinolate content of rapeseed;
Y is the content of sulphur glucoside in the rapeseed in the equation; λ
1～λ
4Be respectively the reflectivity of wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave.
2. the system of a fast detecting thioglycoside content of rape seeds is characterized in that, comprising:
Sample cell (1): be used for the splendid attire rapeseed, the bottom surface is transparent optics window (11);
Spectra collection device (2): be located at optics window (11) under, be used to gather the reflectivity of wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave;
Processing module (3): receive each reflectivity and substitution equation: y=4619 λ
1-6593 λ
2-771.077 λ
3+ 1798 λ
4+ 153.084, calculate glucosinolate content in the rapeseed;
Y is the content of sulphur glucoside in the rapeseed in the equation; λ
1～λ
4Be respectively the reflectivity of wavelength 672nm, 493nm, 841nm, the pairing rapeseed of 423nm light wave.
3. system according to claim 1 is characterized in that: described spectra collection device (2) is made up of the light source (21) and 4 photosensitive components (22) that are in the same plane, and light source (21) is on the same vertical straight line with the center of optics window (11); All photosensitive components (22) are that the center evenly distributes with light source (21), and the plane at their places is parallel with optics window (11).
4. system according to claim 2 is characterized in that: described photosensitive components (22) is made up of the filter plate (221) at top and the light sensor (222) of bottom.
5. system according to claim 1 is characterized in that: the supply unit that is included as the power supply of spectra collection device (2) and processing module (3).
6. system according to claim 1 is characterized in that: described sample cell (1) is provided with handle (4).
7. system according to claim 6 is characterized in that: described spectra collection device (2) is connected with processing module (3) by being located at the inner USB interface data line of handle (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010175823XA CN101858862B (en) | 2010-05-18 | 2010-05-18 | Method and system for quickly detecting thioglycoside content of rape seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010175823XA CN101858862B (en) | 2010-05-18 | 2010-05-18 | Method and system for quickly detecting thioglycoside content of rape seeds |
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| Publication Number | Publication Date |
|---|---|
| CN101858862A true CN101858862A (en) | 2010-10-13 |
| CN101858862B CN101858862B (en) | 2011-07-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010175823XA Expired - Fee Related CN101858862B (en) | 2010-05-18 | 2010-05-18 | Method and system for quickly detecting thioglycoside content of rape seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102692387A (en) * | 2012-06-15 | 2012-09-26 | 南京农业大学 | Method for determining total content of glucosinolates in plants |
| CN103048296A (en) * | 2012-12-19 | 2013-04-17 | 浙江大学 | Method for detecting iron content of chlorella |
| CN103425091A (en) * | 2012-07-20 | 2013-12-04 | 华中农业大学 | Single grain rapeseed quality near infrared spectrum on-line nondestructive testing method and device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1341851A (en) * | 2001-09-28 | 2002-03-27 | 中国农业科学院油料作物研究所 | Quick testing method of rape thio-glycoside and its test board |
| CN2672647Y (en) * | 2003-04-17 | 2005-01-19 | 中国农业科学院油料作物研究所 | Detecting instrument of rape sulfur and erucic acid |
-
2010
- 2010-05-18 CN CN201010175823XA patent/CN101858862B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1341851A (en) * | 2001-09-28 | 2002-03-27 | 中国农业科学院油料作物研究所 | Quick testing method of rape thio-glycoside and its test board |
| CN2672647Y (en) * | 2003-04-17 | 2005-01-19 | 中国农业科学院油料作物研究所 | Detecting instrument of rape sulfur and erucic acid |
Non-Patent Citations (3)
| Title |
|---|
| 《Ann. appl. Biol.》 19911231 K.J.DOUGHTY et al. Variation in the glucosinolate content of oilseed rape (Brassicu napus L.) leaves. II. Response to infection by Alternaria brassicae (Berk.) Sacc. 第469-477页 1-7 第118卷, 2 * |
| 《中国油料作物学报》 20040930 丁小霞等 傅里叶变换近红外光谱技术测定完整油菜籽中芥酸和硫甙含量 第76-79页 1-7 第26卷, 第3期 2 * |
| 《分析测试学报》 20060731 李培武等 油菜叶片中硫甙总量与分量的HPLC测定 第117-120页 1-7 第25卷, 第4期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102692387A (en) * | 2012-06-15 | 2012-09-26 | 南京农业大学 | Method for determining total content of glucosinolates in plants |
| CN103425091A (en) * | 2012-07-20 | 2013-12-04 | 华中农业大学 | Single grain rapeseed quality near infrared spectrum on-line nondestructive testing method and device |
| CN103425091B (en) * | 2012-07-20 | 2015-11-18 | 华中农业大学 | The online lossless detection method of near infrared spectrum of simple grain rapeseed quality and device |
| CN103048296A (en) * | 2012-12-19 | 2013-04-17 | 浙江大学 | Method for detecting iron content of chlorella |
| CN103048296B (en) * | 2012-12-19 | 2015-06-03 | 浙江大学 | Method for detecting iron content of chlorella |
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| Publication number | Publication date |
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| CN101858862B (en) | 2011-07-20 |
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