CN101857884A - Method for producing ethanol by using acetone-butanol fermentation wastewater as ingredient - Google Patents
Method for producing ethanol by using acetone-butanol fermentation wastewater as ingredient Download PDFInfo
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- CN101857884A CN101857884A CN201010196639A CN201010196639A CN101857884A CN 101857884 A CN101857884 A CN 101857884A CN 201010196639 A CN201010196639 A CN 201010196639A CN 201010196639 A CN201010196639 A CN 201010196639A CN 101857884 A CN101857884 A CN 101857884A
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Abstract
The invention relates to a method for producing ethanol by using acetone-butanol fermentation wastewater as an ingredient, which comprises the following steps of: treating the acetone-butanol fermentation wastewater; crushing a starchy material and mixing the treated wastewater and the crushed material; cooking the mixture for liquefying; saccharifying the liquid; performing parent culture; and performing fermentation and distillation. The method has the advantages of greatly reducing environmental pollution, realizing waste recycling, along with low production cost.
Description
Technical field
The present invention relates to a kind of serves as the method for the aquatic producing and ethanol of batching with acetone butanol fermentation waste water, belongs to chemical field.
Background technology
At present, both at home and abroad acetone, butanols are mainly synthetic by petrochemical complex and since world petroleum resource in short supply, crude oil price rises significantly, fermentative Production ethanol becomes the inexorable trend of future development.Domestic many enterprises begin or prepare to start, but the hysteresis of the treatment technology of acetone butanol fermentation waste water has become to restrict one of principal element of this industry development.Solvent in the final fermented liquid of acetone butanol fermentation is at 1.6-2.2%, all the other 97～98% all are waste water, produce a large amount of waste water behind the fermented liquid process wine with dregs column distillation, one ton of solvent of every according to statistics production will produce the waste water about 50～60 tons, this waste water is a kind of very typical trade effluent, chemical oxygen demand (COD) is generally at 20000mg/L, water quality characteristics be " two-supremes " promptly: high organic concentration, high suspended matter concentration.If directly discharging will be very huge to the harm that environment causes.Big owing to the acetone butanol fermentation wastewater flow rate simultaneously, environmental protection treatment cost height, this has greatly limited the development of acetone butanol fermentation industry.So how to realize that the resource utilization of acetone butanol fermentation waste water is extremely urgent.Because acetone butanol fermentation waste water is rich in organic matter, nutritive value is higher, can be utilized by yeast saccharomyces cerevisiae.
Summary of the invention
It is simple to the purpose of this invention is to provide a kind of production technique, low cost, environmental protection with acetone butanol fermentation waste water serve as the batching aquatic producing and ethanol method.
The technical solution used in the present invention is:
A kind of serves as the method for the aquatic producing and ethanol of batching with acetone butanol fermentation waste water, may further comprise the steps:
One, the treatment step of acetone butanol fermentation waste water:
Acetone butanol fermentation waste water filters postcooling to 55-60 ℃ through 80-100 keevil frame, does not perhaps filter and directly is cooled to 55-70 ℃, the waste water after must handling;
Two, the pulverising step of starchy material:
Crossing diameter after starchy material is pulverized is the screen cloth of 1.2-1.8mm, the material after must sieving;
Three, spice step:
With the material after sieving with handle after waste water by mass ratio 1: (2.5-3) mix, the temperature during the waste water batching must mix the back feed liquid at 55-70 ℃;
Four, boiling liquefaction step:
To mix the back feed liquid, and carry out boiling, temperature remains on 90-105 ℃, and adds Ye Huamei 6u/g starchy material, and liquefying time is 80-100 minute, gets boiling liquefaction back feed liquid;
Five, saccharification step:
After boiling liquefaction finishes, above-mentioned boiling liquefaction back feed liquid is cooled to 58-62 ℃, transfer pH4-4.5 with mass concentration 98% vitriol oil, press the 100-150u/g starchy material and add saccharifying enzyme, add urea by the mass percent that accounts for starchy material 0.1-0.18% simultaneously, saccharification time is 30-40 minute, gets the intact mash of saccharification;
Six, plant female culturing step:
The mash that saccharification is intact is cooled to 28-35 ℃, be delivered to kind in female jar, press 0.1% mass percent access active dry yeast of starchy material dry biomass, culture temperature: 28 ℃-30 ℃, plant female maturation after incubation time 8-14 hour, plant female maturation index: cell count 1.0-1.5 * 10
8/ ml, bud ratio 15%-30%, mortality ratio is less than 1%;
Seven, fermentation step:
After treating kind of female maturation, the 10%-20% volume fraction that accounts for fermention medium by kind of female mash will be planted female mash and will be seeded on the fermention medium, be connected to fermentation cylinder for fermentation 60-96 hour, leavening temperature is 28-32 ℃, when wine degree volume fraction greater than 11.5%, the residual sugar mass concentration less than 0.9% the time, the fermentation liquid after the fermentation is delivered to the distillation unit;
Eight, distilation steps:
Adopt common process distillation fermentation liquid to make alcohol.
The residual sugar mass concentration of the acetone butanol fermentation waste water in the described step 1 is 0.1%-0.5%, pH5-7, and the total solvent massfraction 0.01%-0.05% in the fermented liquid, chemical oxygen demand (COD) is 18000-25000mg/L.
Starchy material is one or more in cassava, corn, sweet potato, bajiao banana taro, jerusalem artichoke, the wheat starch in the described step 2.
Advantage of the present invention is: production cost is low, has significantly reduced environmental pollution, has realized refuse reclamation.
Embodiment
Following examples are in order to explanation the present invention, but are not used for limiting the bright scope of we.
Embodiment 1
A kind of serves as the method for the aquatic producing and ethanol of batching with acetone butanol fermentation waste water, may further comprise the steps:
One, the treatment step of acetone butanol fermentation waste water:
The residual sugar mass concentration of getting acetone butanol fermentation waste water is 0.3%, pH5, and the total solvent massfraction 0.01% in the fermented liquid, chemical oxygen demand (COD) is 18000mg/L.The acetone butanol fermentation waste water of above-mentioned condition is filtered postcooling to 55 ℃, the waste water after must handling through 80 keevil frames;
Two, the pulverising step of starchy material:
It is the screen cloth of 1.5mm that diameter is crossed in cassava pulverizing back, the material after must sieving;
Three, spice step:
By 1: 2.8 mix of mass ratio, the temperature during the waste water batching must be mixed the back feed liquid at 55 ℃ with the waste water after material after sieving and the processing;
Four, boiling liquefaction step:
To mix the back feed liquid, and carry out boiling, temperature remains on 90 ℃, and adds Ye Huamei 6u/g starchy material, and liquefying time is 80 minutes, gets boiling liquefaction back feed liquid;
Five, saccharification step:
After boiling liquefaction finishes, above-mentioned boiling liquefaction back feed liquid is cooled to 58 ℃, transfers pH4-4.5 with mass concentration 98% vitriol oil, press the 100u/g cassava and add saccharifying enzyme, add urea by the mass percent that accounts for cassava 0.1% simultaneously, saccharification time is 30 minutes, gets the intact mash of saccharification;
Six, plant female culturing step:
The mash that saccharification is intact is cooled to 28 ℃, be delivered to kind in female jar, insert active dry yeast, culture temperature: 28 ℃ with 0.1% mass percent by the cassava dry biomass, incubation time is planted female ripe after 8 hours, plant female maturation index: cell count 1.0-1.5 * 10
8/ ml, bud ratio 15%-30%, mortality ratio is less than 1%;
Seven, fermentation step:
After treating kind of female maturation, 10% volume fraction that accounts for fermention medium by kind of female mash will be planted female mash and will be seeded on the fermention medium, be connected to fermentation cylinder for fermentation 60 hours, leavening temperature is 28 ℃, when wine degree volume fraction greater than 12%, the residual sugar mass concentration less than 0.8% the time, the fermentation liquid after the fermentation is delivered to the distillation unit;
Eight, distilation steps:
Adopt common process distillation fermentation liquid to make alcohol.
Embodiment 2
A kind of serves as the method for the aquatic producing and ethanol of batching with acetone butanol fermentation waste water, may further comprise the steps:
One, the treatment step of acetone butanol fermentation waste water:
The residual sugar mass concentration of getting acetone butanol fermentation waste water is 0.3%, pH6, and the total solvent massfraction 0.03% in the fermented liquid, chemical oxygen demand (COD) is 21000mg/L.Above-mentioned acetone butanol fermentation waste water is filtered postcooling to 58 ℃, the waste water after must handling through 90 keevil frames;
Two, the pulverising step of starchy material:
Crossing diameter after corn is pulverized is the screen cloth of 1.5mm, the material after must sieving;
Three, spice step:
By 1: 2.5 mix of mass ratio, the temperature during the waste water batching must be mixed the back feed liquid at 63 ℃ with the waste water after material after sieving and the processing;
Four, boiling liquefaction step:
To mix the back feed liquid, and carry out boiling, temperature remains on 100 ℃, and adds Ye Huamei 6u/g corn, and liquefying time is 90 minutes, gets boiling liquefaction back feed liquid;
Five, saccharification step:
After boiling liquefaction finishes, above-mentioned boiling liquefaction back feed liquid is cooled to 60 ℃, transfers pH4-4.5 with mass concentration 98% vitriol oil, press the 120u/g corn and add saccharifying enzyme, add urea by the mass percent that accounts for corn 0.14% simultaneously, saccharification time is 35 minutes, gets the intact mash of saccharification;
Six, plant female culturing step:
The mash that saccharification is intact is cooled to 31 ℃, be delivered to kind in female jar, press 0.1% mass percent access active dry yeast of corn dry biomass, culture temperature: 29 ℃, incubation time is planted female ripe after 11 hours, plant female maturation index: cell count 1.0-1.5 * 10
8/ ml, bud ratio 15%-30%, mortality ratio is less than 1%;
Seven, fermentation step:
After treating kind of female maturation, 15% volume fraction that accounts for fermention medium by kind of female mash will be planted female mash and will be seeded on the fermention medium, be connected to fermentation cylinder for fermentation 84 hours, leavening temperature is 30 ℃, when wine degree volume fraction greater than 12.5%, the residual sugar mass concentration less than 0.9% the time, the fermentation liquid after the fermentation is delivered to the distillation unit;
Eight, distilation steps:
Adopt common process distillation fermentation liquid to make alcohol.
Embodiment 3
A kind of serves as the method for the aquatic producing and ethanol of batching with acetone butanol fermentation waste water, may further comprise the steps:
One, the treatment step of acetone butanol fermentation waste water:
The residual sugar mass concentration of getting acetone butanol fermentation waste water is 0.5%, pH7, and the total solvent massfraction 0.05% in the fermented liquid, chemical oxygen demand (COD) is 25000mg/L.Above-mentioned acetone butanol fermentation waste water is filtered postcooling to 60 ℃, the waste water after must handling through 100 keevil frames;
Two, the pulverising step of starchy material:
With crossing diameter after sweet potato and the pulverizing of bajiao banana taro mixture is the screen cloth of 1.8mm, the material after must sieving;
Three, spice step:
By 1: 3 mix of mass ratio, the temperature during the waste water batching must be mixed the back feed liquid at 70 ℃ with the waste water after material after sieving and the processing;
Four, boiling liquefaction step:
To mix the back feed liquid, and carry out boiling, temperature remains on 105 ℃, and adds Ye Huamei 6u/g starchy material (sweet potato and bajiao banana taro mixture), and liquefying time is 100 minutes, gets boiling liquefaction back feed liquid;
Five, saccharification step:
After boiling liquefaction finishes, above-mentioned boiling liquefaction back feed liquid is cooled to 62 ℃, transfer pH4-4.5 with mass concentration 98% vitriol oil, press the 150u/g starchy material and add saccharifying enzyme, add urea by the mass percent that accounts for starchy material 0.18% simultaneously, saccharification time is 40 minutes, gets the intact mash of saccharification;
Six, plant female culturing step:
The mash that saccharification is intact is cooled to 35 ℃, be delivered to kind in female jar, press 0.1% mass percent access active dry yeast of starchy material dry biomass, culture temperature: 30 ℃, incubation time is planted female ripe after 14 hours, plant female maturation index: cell count 1.0-1.5 * 10
8/ ml, bud ratio 15%-30%, mortality ratio is less than 1%;
Seven, fermentation step:
After treating kind of female maturation, 20% volume fraction that accounts for fermention medium by kind of female mash will be planted female mash and will be seeded on the fermention medium, be connected to fermentation cylinder for fermentation 72 hours, leavening temperature is 32 ℃, when wine degree volume fraction greater than 11.5%, the residual sugar mass concentration less than 0.8% the time, the fermentation liquid after the fermentation is delivered to the distillation unit;
Eight, distilation steps:
Adopt common process distillation fermentation liquid to make alcohol.
Embodiment 4
The treatment step of acetone butanol fermentation waste water of the present invention: acetone butanol fermentation waste water does not filter and directly is cooled to 55-60 ℃, the waste water after must handling.Starchy material of the present invention can also be in cassava, corn, sweet potato, bajiao banana taro, jerusalem artichoke, the wheat starch one or more.
Advantage of the present invention: acetone butanol fermentation waste water contains the comparatively nutrients such as rich in protein, residual sugar, this part material can be utilized by distillery yeast, the flowability of acetone butanol fermentation waste water is better because the acetone butanol fermentation batching is rarer simultaneously, utilize the waste liquid of acetone butanol fermentation as the batching water of alcoholic fermentation, carry out alcoholic fermentation, acetone butanol fermentation and alcohol fermentation are coupled, realized the water circuit utilization, greatly reduce simultaneously the discharge capacity of sewage, realized the recycling of waste water.
Claims (3)
1. one kind serves as the method for the aquatic producing and ethanol of batching with acetone butanol fermentation waste water, it is characterized in that may further comprise the steps:
One, the treatment step of acetone butanol fermentation waste water:
Acetone butanol fermentation waste water filters postcooling to 55-70 ℃ through 80-100 keevil frame, does not perhaps filter and directly is cooled to 55-70 ℃, the waste water after must handling;
Two, the pulverising step of starchy material:
Crossing diameter after starchy material is pulverized is the screen cloth of 1.2-1.8mm, the material after must sieving;
Three, spice step:
With the material after sieving with handle after waste water by mass ratio 1: (2.5-3) mix, the temperature during the waste water batching must mix the back feed liquid at 55-70 ℃;
Four, boiling liquefaction step:
To mix the back feed liquid, and carry out boiling, temperature remains on 90-105 ℃, and adds Ye Huamei 6u/g starchy material, and liquefying time is 80-100 minute, gets boiling liquefaction back feed liquid;
Five, saccharification step:
After boiling liquefaction finishes, above-mentioned boiling liquefaction back feed liquid is cooled to 58-62 ℃, transfer pH4-4.5 with mass concentration 98% vitriol oil, press the 100-150u/g starchy material and add saccharifying enzyme, add urea by the mass percent that accounts for starchy material 0.1-0.18% simultaneously, saccharification time is 30-40 minute, gets the intact mash of saccharification;
Six, plant female culturing step:
The mash that saccharification is intact is cooled to 28-35 ℃, be delivered to kind in female jar, press 0.1% mass percent access active dry yeast of starchy material dry biomass, culture temperature: 28 ℃-30 ℃, plant female maturation after incubation time 8-14 hour, plant female maturation index: cell count 1.0-1.5 * 10
8/ ml, bud ratio 15%-30%, mortality ratio is less than 1%;
Seven, fermentation step:
After treating kind of female maturation, the 10%-20% volume fraction that accounts for fermention medium by kind of female mash will be planted female mash and will be seeded on the fermention medium, be connected to fermentation cylinder for fermentation 60-96 hour, leavening temperature is 28-32 ℃, when wine degree volume fraction greater than 11.5%, the residual sugar mass concentration less than 0.9% the time, the fermentation liquid after the fermentation is delivered to the distillation unit;
Eight, distilation steps:
Adopt common process distillation fermentation liquid to make alcohol.
2. according to claim 1 a kind of with acetone butanol fermentation waste water serve as the batching aquatic producing and ethanol method, the residual sugar mass concentration that it is characterized in that the acetone butanol fermentation waste water in the described step 1 is 0.1%-0.5%, pH5-7, total solvent massfraction 0.01%-0.05% in the fermented liquid, chemical oxygen demand (COD) is 18000-25000mg/L.
3. according to claim 1 a kind of serve as the method for the aquatic producing and ethanol of batching with acetone butanol fermentation waste water, it is characterized in that starchy material in the described step 2 is one or more in cassava, corn, sweet potato, bajiao banana taro, jerusalem artichoke, the wheat starch.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102642993A (en) * | 2012-04-28 | 2012-08-22 | 中国科学院广州能源研究所 | Alcohol fermentation wastewater treatment method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1814763A (en) * | 2005-12-20 | 2006-08-09 | 云南大学 | Method for improving alcohol yield fermented from starch material |
| CN101671700A (en) * | 2009-09-28 | 2010-03-17 | 广东中科天元新能源科技有限公司 | Bio butanol co-digestion bio-ethanol production method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1814763A (en) * | 2005-12-20 | 2006-08-09 | 云南大学 | Method for improving alcohol yield fermented from starch material |
| CN101671700A (en) * | 2009-09-28 | 2010-03-17 | 广东中科天元新能源科技有限公司 | Bio butanol co-digestion bio-ethanol production method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102642993A (en) * | 2012-04-28 | 2012-08-22 | 中国科学院广州能源研究所 | Alcohol fermentation wastewater treatment method |
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