CN101851644A - Method for producing lactic acid by using sugar liquor fermentation - Google Patents
Method for producing lactic acid by using sugar liquor fermentation Download PDFInfo
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Abstract
The invention relates to a method for producing lactic acid by using sugar liquor fermentation, which comprises the following steps of: (1) preparing raw materials, namely adding sugar liquor, corn pulp solution and yeast extract into a fermentation tank in a volume ratio of 100: 0.015-0.025: 0.004-0.013; (2) heating and sterilizing the raw materials; (3) adjusting the pH of the solution to be between 5.4 and 5.6; and (4) adding lactic acid bacteria into the solution to perform fermentation, namely cooling the solution obtained in the step (3) to between 48 and 50 DEG C, adding the lactic acid bacteria in a volume which is 10 to 15 percent of the total volume of the solution in the fermentation tank, keeping the temperature in the fermentation tank between 48 and 50 DEG C, continuously introducing sterile air into the fermentation tank in a flow of 0.75 to 4m<3>/h in every ton of solution, keeping the pressure in the fermentation tank between 0.4 and 0.8MPa, stirring the solution, adding saturated solution of Ca(OH)2 into the solution to keep the pH of the solution between 6.0 and 6.2, and detecting the glucose content of the solution, wherein when the glucose content is 0, the fermentation is finished. The method of the invention reduces the fermentation period, and improves the fermentation efficiency; and after the fermentation is finished, the solution contains low residual total sugar and residual reducing sugar and high produced acid.
Description
Technical field
The present invention relates to lactic acid and zymotechnique thereof, particularly a kind of method of utilizing high concentration sugar purified liquor fermentation process of producing lactic acid.
Background technology
Lactic acid is to be raw material with agricultural byproducts, through microbial fermentation, extracts and a kind of organic acid of making, and be the monomer starting material of degradable environment-friendly material poly(lactic acid).Be widely used in numerous areas such as food, beverage, medicine, healthcare products, chemical industry, degradable plastics.
The production of lactic acid mainly contains " chemical synthesis ", " enzyme process is synthetic ", " fermentative Production ", and wherein most widely used then is fermentative Production lactic acid.In fermentative Production lactic acid, a lot of enterprises are because the zymotechnique index is immature at present, and material concentration is controlled unreasonable grade and caused fermentation efficiency low, is in particular in:
(1) fermentation period is long, and most of enterprises fermentation period is about 70 hours;
(2) product acid and acid production rate are low, and it is below the 120g/L that acid is produced in domestic large-scale production, and world scale lactic acid enterprise produces acid can reach 130 ~ 150g/L, and acid production rate is generally 1.5 ~ 2g/Lh;
(3) residual total reducing sugar and residual reducing sugar height, residual total reducing sugar is generally more than 0.4%, and residual reducing sugar is generally more than 0.2%;
Therefore, the most of lactic acid of China enterprise can not large-scale production lactic acid.
Summary of the invention
Technical problem to be solved by this invention is: a kind of method of utilizing sugar liquor fermentation to produce lactic acid is provided, so that the reducing sugar in the fermented feed liquid makes full use of, reduces fermentation period, improve fermentation efficiency; Make after the fermentation ends in the feed liquid residual total reducing sugar and residual reducing sugar is low, it is high to produce acid.
The present invention is achieved through the following technical solutions: a kind of method of utilizing sugar liquor fermentation to produce lactic acid, may further comprise the steps: (1) raw material is prepared: according to volume ratio is sugared clear liquid: corn steep liquor solution: yeast extract paste=100:0.015 ~ 0.025:0.004 ~ 0.013 joins in the fermentor tank, the total sugar degree of described sugared clear liquid is that 210 ~ 240g/L, DE value are 92% ~ 100%, the DX value is 90% ~ 100%, in the described corn steep liquor solution: dry-matter 〉=40%, protein 〉=45%, acidity≤14%; (2) heat sterilization: temperature is that 115 ~ 121 ℃, pressure are 0.07 ~ 0.1Mpa in the described fermentor tank, and the heat sterilization time is 15 ~ 45 minutes; (3) regulate material liquid pH: use Ca(OH)
2Saturated solution to regulate material liquid pH be 5.4 ~ 5.6; (4) adding milk-acid bacteria ferments: the temperature that obtains feed liquid in the step (3) is dropped to 48 ~ 50 ℃, add volume and be 10% ~ 15% milk-acid bacteria of feed liquid cumulative volume in the fermentor tank, keeping the interior temperature of fermentor tank is 48 ~ 50 ℃, and in fermentor tank, continue to feed sterile air, ventilation flow rate is feed liquid 0.75 ~ 4m per ton
3/ h, keeping the fermentor tank internal pressure is 0.4 ~ 0.8Mpa, stirs, by adding Ca(OH)
2Saturated solution to keep material liquid pH be 6.0 ~ 6.2, detect the glucose content in the feed liquid, glucose content is 0 o'clock, fermentation ends.
The above-mentioned method of utilizing sugar liquor fermentation to produce lactic acid, in step (1), sugared clear liquid: corn steep liquor solution: yeast extract paste=100:0.02:0.009, the total sugar degree of described sugared clear liquid is that 230g/L, DE value are 96%, the DX value is 95%.
The above-mentioned method of utilizing sugar liquor fermentation to produce lactic acid, in step (2), temperature is that 118 ℃, pressure are 0.09Mpa in the described fermentor tank, the heat sterilization time is 30 minutes.
The above-mentioned method of utilizing sugar liquor fermentation to produce lactic acid in step (3), is used Ca(OH)
2Saturated solution to regulate material liquid pH be 5.5.
The above-mentioned method of utilizing sugar liquor fermentation to produce lactic acid, in step (4), the temperature that obtains feed liquid in the step (3) is dropped to 49 ℃, add volume and be 13% milk-acid bacteria of feed liquid cumulative volume in the fermentor tank, keeping the interior temperature of fermentor tank is 49 ℃, and in fermentor tank, continue to feed sterile air, ventilation flow rate is feed liquid 2.5m per ton
3/ h, keeping the fermentor tank internal pressure is 0.6Mpa, stirs, by adding Ca(OH)
2Saturated solution to keep material liquid pH be 6.1, detect the glucose content in the feed liquid, glucose content is 0 o'clock, fermentation ends.
Of the present invention have a following beneficial technical effects: this Technology can be used for the large-scale production of lactic acid, in actual production, fermentation period is 45 ~ 47 hours, far below 70 hours former lactic fermentation cycles, and fermentation and acid can reach more than the 180g/L, residual reducing sugar≤0.15%, residual total reducing sugar≤0.35%; Have fermentation efficiency height, short characteristics of cycle.
Embodiment
Embodiment 1
Present embodiment utilizes sugar liquor fermentation to produce the method for lactic acid, may further comprise the steps: (1) raw material is prepared: according to volume ratio is sugared clear liquid: corn steep liquor solution: yeast extract paste=100:0.015:0.004 joins in the fermentor tank, the total sugar degree of described sugared clear liquid is that 210g/L, DE value are 92%, the DX value is 90%, and in the described corn steep liquor solution: dry-matter is 40%, protein is 45%, acidity is 14%; (2) heat sterilization: temperature is that 115 ℃, pressure are 0.07Mpa in the described fermentor tank, and the heat sterilization time is 15 minutes; (3) regulate material liquid pH: use Ca(OH)
2Saturated solution to regulate material liquid pH be 5.4, if because of carelessness Ca(OH)
2Saturated solution add too much, then use the pH of the adjusting feed liquid of 80% lactic acid or 35% hydrochloric acid; (4) adding milk-acid bacteria ferments: the temperature that obtains feed liquid in the step (3) is dropped to 48 ℃, add volume and be 10% milk-acid bacteria of feed liquid cumulative volume in the fermentor tank, keeping the interior temperature of fermentor tank is 48 ℃, and continues to feed sterile air in fermentor tank, and ventilation flow rate is feed liquid 0.75m per ton
3/ h, keeping the fermentor tank internal pressure is 0.4Mpa, stirs, by adding Ca(OH)
2Saturated solution to keep material liquid pH be 6.0, detect the glucose content in the feed liquid, glucose content is 0 o'clock, fermentation ends.
Present embodiment fermented 42 hours altogether, and acid production rate is 4.08g/Lh, produced sour 171.36g/L, and residual total reducing sugar is 0.2%, and residual reducing sugar is 0.08%.
Embodiment 2
Present embodiment utilizes sugar liquor fermentation to produce the method for lactic acid, may further comprise the steps: (1) raw material is prepared: according to volume ratio is sugared clear liquid: corn steep liquor solution: yeast extract paste=100:0.025:0.013 joins in the fermentor tank, the total sugar degree of described sugared clear liquid is that 240g/L, DE value are 100%, the DX value is 100%, and in the described corn steep liquor solution: dry-matter is 50%, protein is 52%, acidity is 10%; (2) heat sterilization: temperature is that 121 ℃, pressure are 0.1Mpa in the described fermentor tank, and the heat sterilization time is 45 minutes; (3) regulate material liquid pH: use Ca(OH)
2Saturated solution to regulate material liquid pH be 5.6, if because of carelessness Ca(OH)
2Saturated solution add too much, then use the pH of the adjusting feed liquid of 80% lactic acid or 35% hydrochloric acid; (4) adding milk-acid bacteria ferments: the temperature that obtains feed liquid in the step (3) is dropped to 50 ℃, add volume and be 15% milk-acid bacteria of feed liquid cumulative volume in the fermentor tank, keeping the interior temperature of fermentor tank is 50 ℃, and continues to feed sterile air in fermentor tank, and ventilation flow rate is feed liquid 4m per ton
3/ h, keeping the fermentor tank internal pressure is 0.8Mpa, stirs, by adding Ca(OH)
2Saturated solution to keep material liquid pH be 6.2, detect the glucose content in the feed liquid, glucose content is 0 o'clock, fermentation ends.
Present embodiment fermented 47 hours altogether, and acid production rate is 4.21g/Lh, produced sour 197.87g/L, and residual total reducing sugar is 0.32%, and residual reducing sugar is 0.09%.
Embodiment 3
Present embodiment utilizes sugar liquor fermentation to produce the method for lactic acid, may further comprise the steps: (1) raw material is prepared: according to volume ratio sugar clear liquid: corn steep liquor solution: yeast extract paste=100:0.02:0.009, the total sugar degree of described sugared clear liquid is that 230g/L, DE value are 96%, the DX value is 95%.
(2) heat sterilization: temperature is that 118 ℃, pressure are 0.09Mpa in the described fermentor tank, and the heat sterilization time is 30 minutes
(3) regulate material liquid pH: use Ca(OH)
2Saturated solution to regulate material liquid pH be 5.5, if because of carelessness Ca(OH)
2Saturated solution add too much, then use the pH of the adjusting feed liquid of 80% lactic acid or 35% hydrochloric acid.
(4) adding milk-acid bacteria ferments: the temperature that obtains feed liquid in the step (3) is dropped to 49 ℃, add volume and be 13% milk-acid bacteria of feed liquid cumulative volume in the fermentor tank, keeping the interior temperature of fermentor tank is 49 ℃, and continues to feed sterile air in fermentor tank, and ventilation flow rate is feed liquid 2.5m per ton
3/ h, keeping the fermentor tank internal pressure is 0.6Mpa, stirs, by adding Ca(OH)
2Saturated solution to keep material liquid pH be 6.1, detect the glucose content in the feed liquid, glucose content is 0 o'clock, fermentation ends.
Present embodiment fermented 46 hours altogether, and acid production rate is 4.11g/Lh, produced sour 189g/L, and residual total reducing sugar is 0.34%, and residual reducing sugar is 0.07%.
Claims (5)
1. method of utilizing sugar liquor fermentation to produce lactic acid, it is characterized in that, may further comprise the steps: (1) raw material is prepared: according to volume ratio is sugared clear liquid: corn steep liquor solution: yeast extract paste=100:0.015 ~ 0.025:0.004 ~ 0.013 joins in the fermentor tank, the total sugar degree of described sugared clear liquid is that 210 ~ 240g/L, DE value are 92% ~ 100%, the DX value is 90% ~ 100%, in the described corn steep liquor solution: dry-matter 〉=40%, protein 〉=45%, acidity≤14%; (2) heat sterilization: temperature is that 115 ~ 121 ℃, pressure are 0.07 ~ 0.1Mpa in the described fermentor tank, and the heat sterilization time is 15 ~ 45 minutes; (3) regulate material liquid pH: use Ca(OH)
2Saturated solution to regulate material liquid pH be 5.4 ~ 5.6; (4) adding milk-acid bacteria ferments: the temperature that obtains feed liquid in the step (3) is dropped to 48 ~ 50 ℃, add volume and be 10% ~ 15% milk-acid bacteria of feed liquid cumulative volume in the fermentor tank, keeping the interior temperature of fermentor tank is 48 ~ 50 ℃, and in fermentor tank, continue to feed sterile air, ventilation flow rate is feed liquid 0.75 ~ 4m per ton
3/ h, keeping the fermentor tank internal pressure is 0.4 ~ 0.8Mpa, stirs, by adding Ca(OH)
2Saturated solution to keep material liquid pH be 6.0 ~ 6.2, detect the glucose content in the feed liquid, glucose content is 0 o'clock, fermentation ends.
2. the method for utilizing sugar liquor fermentation to produce lactic acid according to claim 1, it is characterized in that, in step (1), sugared clear liquid: corn steep liquor solution: yeast extract paste=100:0.02:0.009, the total sugar degree of described sugared clear liquid is that 230g/L, DE value are 96%, the DX value is 95%.
3. the method for utilizing sugar liquor fermentation to produce lactic acid according to claim 1 is characterized in that, in step (2), temperature is that 118 ℃, pressure are 0.09Mpa in the described fermentor tank, and the heat sterilization time is 30 minutes.
4. the method for utilizing sugar liquor fermentation to produce lactic acid according to claim 1 is characterized in that, in step (3), uses Ca(OH)
2Saturated solution to regulate material liquid pH be 5.5.
5. the method for utilizing sugar liquor fermentation to produce lactic acid according to claim 1, it is characterized in that, in step (4), the temperature that obtains feed liquid in the step (3) is dropped to 49 ℃, add volume and be 13% milk-acid bacteria of feed liquid cumulative volume in the fermentor tank, keeping the interior temperature of fermentor tank is 49 ℃, and continues to feed sterile air in fermentor tank, and ventilation flow rate is feed liquid 2.5m per ton
3/ h, keeping the fermentor tank internal pressure is 0.6Mpa, stirs, by adding Ca(OH)
2Saturated solution to keep material liquid pH be 6.1, detect the glucose content in the feed liquid, glucose content is 0 o'clock, fermentation ends.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255955A (en) * | 2015-11-12 | 2016-01-20 | 安徽中粮生化格拉特乳酸有限公司 | Production method for lactic acid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693911A (en) * | 2009-10-22 | 2010-04-14 | 河南金丹乳酸科技有限公司 | Liquor cleaning process for refining sugar from corns |
| CN101736042A (en) * | 2010-01-08 | 2010-06-16 | 合肥工业大学 | Method for producing L-lactic acid |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693911A (en) * | 2009-10-22 | 2010-04-14 | 河南金丹乳酸科技有限公司 | Liquor cleaning process for refining sugar from corns |
| CN101736042A (en) * | 2010-01-08 | 2010-06-16 | 合肥工业大学 | Method for producing L-lactic acid |
Non-Patent Citations (2)
| Title |
|---|
| 《天津轻工业学院学报》 20021231 刘伟等 细菌发酵生产L-乳酸的研究 第11-13页 1-5 , 第4期 2 * |
| 《食品与机械》 20080131 梁宝东等 L-乳酸细菌发酵培养基的优化 第41-45页 1-5 第24卷, 第1期 2 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255955A (en) * | 2015-11-12 | 2016-01-20 | 安徽中粮生化格拉特乳酸有限公司 | Production method for lactic acid |
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Owner name: HE NAN JINDAN LACTIC ACID TECH. CO., LTD. Free format text: FORMER OWNER: HENAN JINDAN LACTIC ACID TECHNOLOGY CO., LTD. Effective date: 20111018 |
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Effective date of registration: 20111018 Address after: No. 08 Dancheng Dan county of Henan province Henan province 477150 Avenue Applicant after: Henan Jindan Lactic Acid Tech Co.,Ltd. Address before: Dan Dancheng County of Henan Province, Henan province 477150 Road No. 08 Henan Jindan Technology Co. Ltd. Applicant before: Henan Jindan Lactic Acid Technology Co., Ltd. |
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