CN101831477A - Method for preparing rice into glucose for injection - Google Patents
Method for preparing rice into glucose for injection Download PDFInfo
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- CN101831477A CN101831477A CN201010181820A CN201010181820A CN101831477A CN 101831477 A CN101831477 A CN 101831477A CN 201010181820 A CN201010181820 A CN 201010181820A CN 201010181820 A CN201010181820 A CN 201010181820A CN 101831477 A CN101831477 A CN 101831477A
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Abstract
The invention relates to a method for preparing rice into glucose for injection. The method comprises the following steps of: removing protein in rice by adopting amylase; then, hydrolyzing and saccharifying the protein of broken rice separately with alpha-amylase, diastatic enzyme and pullulanase; decoloring, removing impurities and filtering by using active carbon; removing ions by using ion exchange columns; crystallizing and drying; and finally obtaining qualified glucose powder for injection. The invention has the advantages of improving the starch conversion rate and the added values and the high-tech contents of rice processing, reducing the production cost for preparing starch into glucose for injection, changing waste into valuable, and promoting the development of rice-processing industrialization.
Description
Technical field
The invention provides a kind of method of preparing rice into glucose for injection, belong to biological pharmacy technical field.
Background technology
Used glucose for injection adopts the W-Gum preparation, and Japan prepares glucose for injection with cassava, and rice (cracking rice) starch was only made edible glucose, does not also make the report of glucose for injection.It is few to contain albumen in the corn, and the starch particle diameter is big.Broken rice contains albumen and reaches 7.0～10.0%, and the little and starch of starch particle diameter becomes reticulated structure closely with protein binding.The gelatinization point of starch small grain size decision starch influences the liquefaction of starch.The corn starch pasting temperature is low, liquefaction easily, and the Starch rice particle diameter is little, gelatinization point is higher, reaches 85 ℃, and tight with protein binding, is difficult for liquefaction.DE reaches more than 98% behind the W-Gum liquefying-saccharifying, and is difficult to behind the Starch rice liquefying-saccharifying thoroughly, and impurity is many, makes very difficulty of glucose for injection.The used a-amylase of starch hydrolysis is a kind of excision enzyme, and the alpha-1, 4-glucosidic key is decomposed easily.Saccharifying enzyme decomposes easily the alpha-1, 4-glucosidic key, decomposes very slow to the alpha-1, 6-glucosidic key.The single-minded decomposition alpha-1, 6-glucosidic of Pullulanase key.Adopt saccharifying enzyme and Pullulanase in the saccharification, low molecular starch products is decomposed fully, greatly improve the DE value of glucose.
Summary of the invention
At the deficiency of above-mentioned technology, the purpose of this invention is to provide a kind of method of preparing rice into glucose for injection.
The method of a kind of preparing rice into glucose for injection of the present invention, employing amylase removes the albumen in the rice, with a-amylase, saccharifying enzyme and Pullulanase gradation the starch of cracking rice is hydrolyzed and saccharification then, the decolorizing with activated carbon removal of impurities is filtered, ion exchange column removes ion, crystallizing and drying obtains qualified glucose for injection powder at last; Its concrete production technique is as follows:
(1), rice is pulverized fineness requirement 60～100 orders;
(2), size mixing: the Starch rice after the pulverizing is pressed solid-liquid ratio and was added distilled water in 1: 3～1: 7, soaks 4～8h;
(3), liquefaction: add high temperature a-amylase 40～60U/g, press 0.1～0.3% of rice weight and add CaCl
2Regulate pH to 6.0～6.5 with liming again, be heated to 90～100 ℃, reaction 30～50mi n, at this moment, it is colourless to add Iod R, and the DE value is 15～20, and whizzer 800～2500r/min separates liquefier;
(4), saccharification: adding saccharifying enzyme 100～150U/g and Pullulanase is 0.05～0.25apu/g, after HCL adjusting pH value 4.0～5.5, under tank pressure 0.3MPa, 45～60 ℃ of temperature, saccharification 48～60h, terminal point iodine colour response is constant, check dextrin with raw spirit, should be negative;
(5), decolorization filtering for the first time: decolorization and impurity removal by active carbon, activated carbon dosage is about 1～3%, is heated to 70～85 ℃, time 20～40mi n, whizzer 800～2500r/min separates saccharification liquid, No. 6 core filtering separation saccharification liquid;
(6), ion-exchange: remove Ca by anion-cation exchange resin
2+, Mg
2+, Fe
3+, SO
4 2-, CL
-Plasma;
(7), decolorization filtering for the second time: with the solution concentration after the ion-exchange, add 1～3% activated carbon, be heated to 70～85 ℃ by concentration, time 20～40min, whizzer 800～2500r/mi n separates saccharification liquid, No. 6 core filtering separation saccharification liquid;
(8), crystallization: crystallization is operated under 100 grades of aseptic conditions and is carried out, Tc is controlled at 25～50 ℃, adopt gradient crystallization, two sections temperature are respectively 35～45 ℃, 25～35 ℃, cooling rate is controlled at 0.2～0.4 ℃/h, 0.3～0.6 ℃/h, fluidised bed drying is used in glucose crystallization 800～2500r/mi n centrifugation.
Advantage of the present invention is: improve the transformation efficiency of starch, improve the added value and the high-tech content of rice processing, reduce the production cost that starch prepares glucose for injection, turn waste into wealth, promote the development of rice secondary industryization.
Embodiment
Rice directly washes the back with water and soaks 4h, is ground into 60 orders, presses solid-liquid ratio and adds distilled water at 1: 5, is preheated to 95 ℃, presses 0.3% of rice weight and adds CaCL
2, regulate pH to 6.2; Add high temperature a-amylase 60U/g, stir while feeding in raw material and wave, temperature is retained to 95 ℃, reaction times 40min.At this moment, it is colourless to add Iod R, and DE (dextrose equivalent value) value is 20, the 1000r/min centrifugation.
Liquefaction back tank pressure 1.4150MPa is warmed up to 110 ℃, and 10min kills enzyme.
Saccharification: after HCL adjusting pH value 4.8, adding saccharifying enzyme 120U/g and Pullulanase is 0.25apu/g, under tank pressure 0.3MPa, and 60 ℃ of temperature, saccharification 48h, it is reactionless that terminal point adds iodine.
Neutralization is filtered: below the saccharification liquid cooling to 90 ℃, pH transfers to 5.0, and the exhausted waste active carbon is an amount of when adding decolorization filtering, to help filtering and reclaim adherent dense liquid glucose in the useless charcoal, stirs the 30min after-filtration.
Concentrate for the first time: concentrate with vacuum-evaporation, vacuum tightness requires more than the 0.065MPa, and the supplied materials before concentrating will transfer to 4.5 with pH with rare HCL, is concentrated into relative density more than 1.200.
Decolorization filtering: supplied materials is heated to 75 ℃, adds gac, consumption is about 10%, and destainer requires to be as the criterion to reach form and aspect.1000r/min centrifuging after this temperature stirs decolouring 30min, filtrate asks for a clarification, and does not leak charcoal and foreign matter.
Ion-exchange: ion-exchange is adopted 3 groups, with sun-male-female-the moon--sun-male-female is a group bed mode, be resin cation (R.C.) at last, the PH of resin cation (R.C.) is 6.0, the PH of anionic resin is 7.5, flow velocity is per hour removed unnecessary ion in the decarburization saccharification liquid for 3 times of the resin bed volume, removes Ca by anion-cation exchange resin
2+, Mg
2+, Fe
3+, SO
4 2-, CL
-Plasma.
Concentrate decolouring the second time: high more good more with the dense vacuum tightness of vacuum-evaporation, require more than the 0.078MPa, the gac by concentration adding 10% is heated to 75 ℃, time 30min, whizzer 1000r/min separates saccharification liquid, No. 6 core filtering separation saccharification liquid.
Crystallization: crystallization is operated under 100 grades of aseptic conditions and is carried out, and Tc is controlled at below 50 ℃, adopts gradient crystallization, and two sections temperature are respectively 35～45 ℃, and 25～35 ℃, cooling rate is controlled at 0.2～0.4 ℃/h, 0.3～0.6 ℃/h;
Separate and drying: glucose crystallization 1000r/min centrifugation, fluidized drying is carried out drying with fluidized-bed for 60 ℃ in temperature, and moisture content of finished products requires below 10%.
Claims (1)
1. the method for preparing rice into glucose for injection is characterized in that, its concrete production technique is as follows:
(1), rice is pulverized fineness requirement 60～100 orders;
(2), size mixing: the Starch rice after the pulverizing is pressed solid-liquid ratio and was added distilled water in 1: 3～1: 7, soaks 4～8h;
(3), liquefaction: add high temperature a-amylase 40～60U/g, press 0.1～0.3% of rice weight and add CaCl
2Regulate pH to 6.0～6.5 with liming again, be heated to 90～100 ℃, reaction 30～50min, at this moment, it is colourless to add Iod R, and the DE value is 15～20, and whizzer 800～2500r/min separates liquefier;
(4), saccharification: adding saccharifying enzyme 100～150U/g and Pullulanase is 0.05～0.25apu/g, after HCL adjusting pH value 4.0～5.5, under tank pressure 0.3MPa, 45～60 ℃ of temperature, saccharification 48～60h, terminal point iodine colour response is constant, check dextrin with raw spirit, should be negative;
(5), decolorization filtering for the first time: decolorization and impurity removal by active carbon, activated carbon dosage is about 1～3%, is heated to 70～85 ℃, time 20～40min, whizzer 800～2500r/min separates saccharification liquid, No. 6 core filtering separation saccharification liquid;
(6), ion-exchange: remove Ca by anion-cation exchange resin
2+, Mg
2+, Fe
3+, SO
4 2～, CL
～Plasma;
(7), decolorization filtering for the second time: with the solution concentration after the ion-exchange, add 1～3% activated carbon, be heated to 70～85 ℃ by concentration, time 20～40min, whizzer 800～2500r/min separates saccharification liquid, No. 6 core filtering separation saccharification liquid;
(8), crystallization: crystallization is operated under 100 grades of aseptic conditions and is carried out, Tc is controlled at 25～50 ℃, adopt gradient crystallization, two sections temperature are respectively 35～45 ℃, 25～35 ℃, cooling rate is controlled at 0.2～0.4 ℃/h, 0.3～0.6 ℃/h, fluidised bed drying is used in glucose crystallization 800～2500r/min centrifugation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010181820A CN101831477A (en) | 2010-05-21 | 2010-05-21 | Method for preparing rice into glucose for injection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010181820A CN101831477A (en) | 2010-05-21 | 2010-05-21 | Method for preparing rice into glucose for injection |
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| CN101831477A true CN101831477A (en) | 2010-09-15 |
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| CN201010181820A Pending CN101831477A (en) | 2010-05-21 | 2010-05-21 | Method for preparing rice into glucose for injection |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061321A (en) * | 2010-11-17 | 2011-05-18 | 湖南中医药大学 | Method for preparing glucose raw material for injection from broken rice and preparations |
| CN102703547A (en) * | 2012-06-14 | 2012-10-03 | 万福生科(湖南)农业开发股份有限公司 | Method for producing glucose bulk drug for injection by using rice |
| CN102876758A (en) * | 2012-09-28 | 2013-01-16 | 浙江华康药业股份有限公司 | Method for preparing fructose syrup |
| CN102994654A (en) * | 2012-12-17 | 2013-03-27 | 西王药业有限公司 | Glucose crystallizer and production technology |
| CN103232522A (en) * | 2013-04-19 | 2013-08-07 | 武汉工业学院 | Method for coproducing and preparing malt syrup and proteins from broken rice |
| CN103525887A (en) * | 2013-10-21 | 2014-01-22 | 诸城东晓生物科技有限公司 | Energy-saving environment-friendly crystalline dextrose production method |
| CN110982861A (en) * | 2019-12-24 | 2020-04-10 | 山东省鲁洲食品集团有限公司 | Method for improving filtration speed of crystalline glucose saccharification liquid |
-
2010
- 2010-05-21 CN CN201010181820A patent/CN101831477A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061321A (en) * | 2010-11-17 | 2011-05-18 | 湖南中医药大学 | Method for preparing glucose raw material for injection from broken rice and preparations |
| CN102703547A (en) * | 2012-06-14 | 2012-10-03 | 万福生科(湖南)农业开发股份有限公司 | Method for producing glucose bulk drug for injection by using rice |
| CN102876758A (en) * | 2012-09-28 | 2013-01-16 | 浙江华康药业股份有限公司 | Method for preparing fructose syrup |
| CN102994654A (en) * | 2012-12-17 | 2013-03-27 | 西王药业有限公司 | Glucose crystallizer and production technology |
| CN102994654B (en) * | 2012-12-17 | 2014-05-14 | 西王药业有限公司 | Glucose crystallizer and production technology |
| CN103232522A (en) * | 2013-04-19 | 2013-08-07 | 武汉工业学院 | Method for coproducing and preparing malt syrup and proteins from broken rice |
| CN103232522B (en) * | 2013-04-19 | 2015-04-22 | 武汉工业学院 | Method for coproducing and preparing malt syrup and proteins from broken rice |
| CN103525887A (en) * | 2013-10-21 | 2014-01-22 | 诸城东晓生物科技有限公司 | Energy-saving environment-friendly crystalline dextrose production method |
| CN103525887B (en) * | 2013-10-21 | 2015-09-09 | 诸城东晓生物科技有限公司 | A kind of crystalline dextrose production method of energy-conserving and environment-protective |
| CN110982861A (en) * | 2019-12-24 | 2020-04-10 | 山东省鲁洲食品集团有限公司 | Method for improving filtration speed of crystalline glucose saccharification liquid |
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Application publication date: 20100915 |