CN101831419A - Method for preparing spherical tannin carrier - Google Patents
Method for preparing spherical tannin carrier Download PDFInfo
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- CN101831419A CN101831419A CN 201010139739 CN201010139739A CN101831419A CN 101831419 A CN101831419 A CN 101831419A CN 201010139739 CN201010139739 CN 201010139739 CN 201010139739 A CN201010139739 A CN 201010139739A CN 101831419 A CN101831419 A CN 101831419A
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Abstract
The invention relates to a method for preparing a spherical tannin carrier. The preparation process comprises the following steps: 1), preparing tannin solution at a mass-volume concentration of 3 to 20 percent with distilled water; 2), adding the tannin solution into a dispersion medium organic phase, and obtaining mixed suspension after uniform dispersion; 3) slowing adding a crosslinker in an amount of 5 to 30 percent based on the total volume of the mixed suspension to continue to perform a reaction for 0.5 to 3 hours, and filtering the reaction product to obtain a primary spherical tannin carrier product; and 4) washing the primary spherical tannin carrier product with ethanol, ethyl ether, methanol ad distilled water in turn, and drying the primary tannin carrier product under vacuum to obtain the spherical tannin carrier. The spherical tannin carrier prepared by the method has uniform shape, large surface area and high adsorbability.
Description
Technical field
The present invention relates to a kind of preparation method of spherical tannin carrier.
Background technology
Tannin (Tannins) is a glucose galloyl compound, and the structure of its polyphenol hydroxyl has been given its a series of unique chemical characteristics and physiologically active, if can combine the sorbing material of these compounds of Chang Zuowei with protein, alkaloid, polysaccharide.Tannin or the biomass that are rich in tannin are developed recently main raws of biomass adsorbent rapidly.China has abundant tannin resource, but the abundant tannin of reserves also is far from obtaining extensive studies and application as starch, Mierocrystalline cellulose, chitosan.Prepare big, the good adsorption performance spherical tannin carrier of shape homogeneous, surface-area that meets the industrial production needs, be used for fixing biological enzyme, have favorable industrial application prospect.
Summary of the invention
The purpose of this invention is to provide a kind of abundant raw material, with low cost, physical strength is high, the spherical tannin carrier that chemical property is stable, be fit to industrial application.
For realizing that the technical scheme that purpose of the present invention adopts is: be raw material with the tannin, obtain quality volume (mg/ml) than being the tanning solution of 3～20% concentration with dissolved in distilled water; Then under stirring condition, tanning solution is added in the dispersion medium organic phase, the volume of organic phase is 1 to 5 times of tanning solution volume, treat to form suspension behind the homodisperse, the linking agent that slowly adds suspension cumulative volume 5～30%, continue reaction 0.5～3 hour, filter and remove filtrate, obtain spherical tannin carrier head product; Adopt ethanol, ether, methyl alcohol and distilled water that spherical tannin carrier head product is washed successively,, obtain the spherical tannin carrier through vacuum-drying.
The mass volume ratio concentration of described tanning solution is preferably 5～10%, and the best is 7～8%.
Dispersion medium organic phase of the present invention is meant any one or the arbitrarily several mixing in whiteruss, gasoline, toluene, tetracol phenixin or the hexanaphthene.
Linking agent of the present invention is meant any one or any several mixture in formaldehyde, glutaraldehyde, oxalic dialdehyde or the tolylene diisocyanate.
Spherical tannin support shapes homogeneous prepared in accordance with the present invention, surface-area are big, have the good adsorption performance.
Description of drawings
Fig. 1 is a spherical tannin carrier scanning electron microscope picture of the present invention.
Embodiment
Take by weighing the 5g Weibull, obtain the tanning solution that mass volume ratio is 10% concentration with the 50ml dissolved in distilled water.Under stirring condition, tanning solution joined in the 100ml whiteruss form suspendible, the volume ratio of organic phase and tanning solution is 2:1.Dispersed with stirring 2 hours slowly adds the formaldehyde solution of 15ml, continues reaction 1 hour, filtration obtains spherical tannin carrier head product, spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier.
Embodiment 2
Take by weighing the 5g Weibull, obtain the tanning solution that mass volume ratio is 20% concentration with the 25ml dissolved in distilled water.Under stirring condition, tanning solution is joined in the 75ml dispersion medium organic phase whiteruss, the volume ratio of organic phase and tanning solution is 3:1.Dispersed with stirring 5 hours slowly adds the formaldehyde solution of 10ml, continues reaction 2 hours, filtration obtains spherical tannin carrier head product, spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier of the scope of varying in size.
Embodiment 3
Take by weighing the 5g Weibull, obtain the tanning solution that mass volume ratio is 5% concentration with the 100ml dissolved in distilled water; Under stirring condition, tanning solution is added in the 200ml toluene, the volume ratio of organic phase and tanning solution is 2:1, dispersed with stirring 2 hours slowly adds the glutaraldehyde solution of 30ml, continues reaction 1 hour, filtration obtains spherical tannin carrier head product, spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier of the scope of varying in size.
Embodiment 4
Take by weighing the 5g Weibull, obtain the tanning solution that mass volume ratio is 20% concentration with the 25ml dissolved in distilled water; Under stirring condition, tanning solution is added in the 125ml toluene, the volume ratio of organic phase and tanning solution is 5:1, dispersed with stirring 5 hours slowly adds the glutaraldehyde solution of 22.5ml, continues reaction 1 hour, filtration obtains spherical tannin carrier head product, spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier of the scope of varying in size.
Embodiment 5
Take by weighing the 5g Weibull, obtain the tanning solution that mass volume ratio is 20% concentration with the 25ml dissolved in distilled water; Under stirring condition, tanning solution is added in the 75ml hexanaphthene, the volume ratio of organic phase and tanning solution is 3:1, dispersed with stirring 5 hours slowly adds the glutaraldehyde solution of suspended matter cumulative volume 15ml, continues reaction 1 hour, filtration obtains spherical tannin carrier head product, spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier of the scope of varying in size.
Embodiment 6
The preparation of spherical tannin
Take by weighing the 5g Weibull, obtain the tanning solution that mass volume ratio is 7.7% concentration with the 65ml dissolved in distilled water; Under stirring condition, tanning solution is added in the whiteruss of 162.5ml, the volume ratio of organic phase and tanning solution is 5:2, dispersed with stirring 2 hours, slowly the glutaraldehyde solution of 34ml continues reaction 1.5 hours, filtration obtains spherical tannin carrier head product, spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier of the scope of varying in size.
Embodiment 7
Take by weighing the 5g Weibull, obtain the tanning solution that mass volume ratio is 7.7% concentration with the 65ml dissolved in distilled water; Under stirring condition, tanning solution is added in the 130ml93 gasoline, the volume ratio of organic phase and water is 2:1, dispersed with stirring 2.5 hours, slowly the glyoxal solution of 30ml continues reaction 1.5 hours, filtration obtains spherical tannin carrier head product, spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier of the scope of varying in size.
The spherical tannin preparing carriers alpha-amylase immobilized that makes with present embodiment
Get in the α-Dian Fenmei solution that 2.5g spherical tannin carrier joins 50ml, the ultimate density of α-Dian Fenmei is 2.5mg/ml, in 10 ℃ of absorption 10 hours, adding concentration is 20% glutaraldehyde solution, the final volume that makes glutaraldehyde solution and total solution is than being 1:30, in 10 ℃ crosslinked 3 hours, filter, be 7 phosphate buffer solution washing and blot with the pH value, promptly make the spherical tannin alpha-amylase immobilized.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (6)
1. the preparation method of a spherical tannin carrier comprises the steps:
1) be the tanning solution of 3-20% concentration with distilled water preparation mass volume ratio;
2) tanning solution is added in the dispersion medium organic phase, treat to form suspended matter behind the homodisperse;
3) slowly add the linking agent of suspended matter cumulative volume 5-30%, continue reaction 0.5-3 hour, filter, obtain spherical tannin carrier head product;
4) spherical tannin carrier head product adopts ethanol, ether, methyl alcohol and distilled water wash successively, and vacuum-drying obtains the spherical tannin carrier of the scope of varying in size.
2. the preparation method of spherical tannin carrier as claimed in claim 1 is characterized in that the mass volume ratio concentration of described tanning solution is preferably 5-10%, and the best is 7-8%.
3. the preparation method of spherical tannin carrier as claimed in claim 1 is characterized in that the volume ratio of described organic phase and water is 1:0.2～1, is preferably between 1:0.25～0.6, and the best is between 1:0.3～0.5.
4. the preparation method of spherical tannin carrier as claimed in claim 1 is characterized in that described linking agent is meant any one or any several mixture in formaldehyde, glutaraldehyde, oxalic dialdehyde or the tolylene diisocyanate.
5. as the preparation method of claim 1 and 4 described spherical tannin carriers, it is characterized in that described linking agent accounts for the mixture cumulative volume and is preferably 10-25%, the best is 15-20%.
6. the preparation method of spherical tannin carrier as claimed in claim 1 is characterized in that described organic phase is any one or the arbitrarily several mixing in whiteruss, gasoline, toluene, tetracol phenixin or the hexanaphthene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010139739 CN101831419A (en) | 2010-04-06 | 2010-04-06 | Method for preparing spherical tannin carrier |
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| CN 201010139739 CN101831419A (en) | 2010-04-06 | 2010-04-06 | Method for preparing spherical tannin carrier |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102584435A (en) * | 2012-02-08 | 2012-07-18 | 福建师范大学 | Preparation method for slow-release fertilizer with polyphenol biomass as carrier |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382787A (en) * | 2001-04-25 | 2002-12-04 | 马连清 | Composition containing dannin and its appication |
| CN101215366A (en) * | 2008-01-16 | 2008-07-09 | 北京林业大学 | Tannin chelating resin and preparing method thereof |
-
2010
- 2010-04-06 CN CN 201010139739 patent/CN101831419A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382787A (en) * | 2001-04-25 | 2002-12-04 | 马连清 | Composition containing dannin and its appication |
| CN101215366A (en) * | 2008-01-16 | 2008-07-09 | 北京林业大学 | Tannin chelating resin and preparing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 《中国优秀硕士学位论文全文数据库》 20081215 杜洁 反相悬浮聚合制备单宁螯合树脂及吸附机理研究 摘要及第13页 1-6 , 第12期 2 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102584435A (en) * | 2012-02-08 | 2012-07-18 | 福建师范大学 | Preparation method for slow-release fertilizer with polyphenol biomass as carrier |
| CN102584435B (en) * | 2012-02-08 | 2013-11-20 | 福建师范大学 | Preparation method for slow-release fertilizer with polyphenol biomass as carrier |
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Open date: 20100915 |