CN101823072A - Method for strengthening methane oxidation of covering layer of landfill site - Google Patents
Method for strengthening methane oxidation of covering layer of landfill site Download PDFInfo
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- CN101823072A CN101823072A CN201010157441A CN201010157441A CN101823072A CN 101823072 A CN101823072 A CN 101823072A CN 201010157441 A CN201010157441 A CN 201010157441A CN 201010157441 A CN201010157441 A CN 201010157441A CN 101823072 A CN101823072 A CN 101823072A
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention provides a method for strengthening methane oxidation of a covering layer of a landfill site and relates to a method capable of enriching methanotroph microbe inside mineralized garbage. The method comprises the following steps of: weighting sugar in proportion of adding 0.5 to 3kg of sugar into 1 ton of the mineralized garbage; adding 1 to 3 mass parts of sugar into 100 mass parts of water, stirring the mixture to prepare sugar solution, and adding the sugar solution into the mineralized garbage; uniformly mixing the mixture, and acclimating the mixture for 7 to 10 days to obtain a covering layer material; and finally, uniformly spreading the covering layer material on the surface of the domestic garbage through the conventional covering process according to 40 to 60cm of thickness of a final covering layer after a domestic garbage landfill unit finishes operation. The methane oxidation efficiency is improved to 810g/(m2.d) through detection. The method realizes treating waste with waste, has low cost, improves the methane oxidation efficiency by 2 to 3 times compared with common covering materials of the landfill site, and realizes emission reduction of greenhouse gases of the landfill site. The method has obvious social benefit and economic benefit, can be widely used for the domestic garbage landfill site, and is particularly suitable for strengthening methane oxidation covering layers of medium-sized and small domestic garbage landfill sites.
Description
Technical field
The present invention relates to a kind of method of strengthening the methane oxidation of covering layer of landfill site ability, specifically is to have a liking for the methanogen method in enrichment mineralized waste inside, belongs to environmental pollution prevention and control technology and reduction of greenhouse gas discharge technical field.
Background technology
Methane in the atmosphere is to be only second to CO
2Important greenhouse gases, its greenhouse effects contribution rate reaches 26%.The discharge capacity of the annual methane in the whole world reaches 5.35 * 10
8T, wherein artificial source discharge of methane amount is 3.75 * 10
8T.Debirs in the household refuse landfill sites decompose under anaerobic state, can produce landfill gas.Research in recent years thinks that household refuse landfill sites has become one of maximum anthropogenic discharge source of methane in the atmosphere.
Methane-oxidizing bacteria can be with methane as the carbon source and the energy, with oxygen is electron acceptor, by methane MO, methanol dehydrogenase, formaldehyde dehydrogenase and four step of hydrogenlyase catalytic reaction, methane conversion in the household refuse landfill sites is become carbon dioxide and forms cytoplasm.Carbon dioxide is the metabolite of thalline, and namely for methane is oxidized into carbon dioxide at last.There are some researches show that the function methane oxidation of covering layer can effectively promote the terminal conversion of artificial source methane, methane-oxidizing bacteria quantity is maximum in 0～30cm soil layer, and activity is the strongest, and methane oxidation speed reaches as high as 290g/ (m
2D).The principal element that influences the function methane oxidation of covering layer has soil texture, nutrient situation, humidity, temperature, methane and concentration of oxygen, NH
4 +And soil pH value etc.Although to function methane oxidation of covering layer microorganism and to influence the research of methane oxidation factor commonplace, but the efficient of carrying out methane oxidation by cover layer is not very good, and the problem of this maximum wherein is exactly that many bottlenecks have limited the application development of methane-oxidizing bacteria because the cell speed of growth of methane-oxidizing bacteria is slow, density is low, catalytic reaction is subjected to that reducibility coenzyme influences etc.Methane-oxidizing bacteria makes thalline enrichment and expansion cultivation means be difficult to use on engineering owing to being that carbon source is difficult to separate with the method for agar plate with methane or methyl compound only like this.Therefore use in order to ensure the engineering of methane oxidation of covering layer, must improve the tectal oxygenation efficiency of unit.
Summary of the invention
It is simple, with low cost and be applicable to that middle-size and small-size household refuse landfill sites improves the method for methane oxidation of covering layer efficient to the objective of the invention is to disclose a kind of technology.
In order to achieve the above object, the present invention be subjected to mention in the uncle's introduction of outstanding bacterium handbook about Methylomonadaceae " many bacteriums are to utilize many carbon compounds and single carbon compound; rather than specially rely on methane or methyl alcohol as the carbon source and the energy " inspire, find after the degraded of more concern methane-oxidizing bacteria to many carbon compounds: add saccharide compound and can strengthen tectal methane oxidation ability, this discovery has fundamentally solved the bottleneck that traditional methane oxidation of covering layer ability is difficult to improve, and has avoided discharging the greenhouse effects that methane causes.And then to finding in the research of mineralized waste that adding saccharide compounds such as sucrose, glucose in mineralized waste is carbon source, through domestication after a while, the methane oxidation effect of mineralized waste has obtained tangible reinforcement.
Being defined as of the mineralized waste of indication of the present invention: in butt, when the landfill yard house refuse by after 8 years or the above biodegradation, its solid product be reduced to the content of organic matter 9～15%, ion exchange capacity 120～140mmol/100g, bacterial population 1～9 * 10
6Nearly neutral 7.5, the saturated waterpower coefficient of permeability K of individual/g, pH value
SBecome stable mineralized waste during 1～1.3cm/min, its adsorption specific surface area 5～6m
2/ g, total nitrogen 0.5%, total phosphorus and total potassium are about 1%.Domestication contains a large amount of not only oxidable methane but also can utilize the microorganism of sugar mineralized waste through the methane under the landfill condition over a long time.Therefore, be the function cladding material with the mineralized waste, strengthen by sugar, can effectively promote methane oxidation of covering layer efficient.
Concrete technology is as follows:
At first, measure sugar, then, 1～3 portion of quality sugar joined in 100 parts of quality water stir, be configured as sugar juice according to the ratio that adds 0.5～3kg sugar in the mineralized waste per ton; Sugar juice added in the mineralized waste mix, become covering layer material through 7～10 days domestication (static under the normal temperature) again; At last, after the consumer waste filling and embedding unit process finishes, according to the conventional covering process of end cover layer 40-60cm thickness with the even spreading of covering layer material on the house refuse surface; After testing, methane oxidation efficient is increased to 810g/ (m
2D).
Described mineralized waste is a landfill more than 8 years, and particle diameter is 0.5～4mm, and moisture content is 20～30% mineralized waste.
Described sugar is commercially available sucrose, glucose, mannose, fructose, raffinose, lactose or starch.
The present invention has following advantage:
1, because the raw material of utilization of the present invention is to have added the covering layer material through taming behind the sugar juice, therefore effectively promote methane oxidation of covering layer efficient, and, technology is simple, be the treatment of wastes with processes of wastes against one another, realize the method for landfill yard reduction of greenhouse gas discharge, have obvious social and economic benefit.
2, the present invention is carried out operation according to the conventional covering process of end cover layer 40-60cm, methane oxidation efficient can reach 810g/ (m
2D), the methane oxidation efficient than common landfill yard cladding material exceeds 2～3 times.
3, cost of the present invention is low, and technology is simple, improves methane oxidation of covering layer efficient particularly suitable for middle-size and small-size household refuse landfill sites, can promote widely.
Description of drawings
Fig. 1 is a process chart of the present invention
Fig. 2 be in the mineralized waste of the present invention Mixed Microbes to the utilization of different sugar
Fig. 3 is that interpolation different sugar of the present invention is to mineralized waste Mixed Microbes concentration affects
Fig. 4 is the influence curve figure of interpolation sucrose of the present invention to the mineralized waste oxidizing of methylene
Fig. 5 is the influence curve figure of interpolation starch of the present invention to the mineralized waste oxidizing of methylene
Fig. 6 is the influence curve figure of interpolation mannose of the present invention to the mineralized waste oxidizing of methylene
Fig. 7 is the influence curve figure of interpolation lactose of the present invention to the mineralized waste oxidizing of methylene
Fig. 8 is the influence curve figure of interpolation fructose of the present invention to the mineralized waste oxidizing of methylene
Fig. 9 is the influence curve figure of interpolation raffinose of the present invention to the mineralized waste oxidizing of methylene
The specific embodiment
Choose 7 kinds of sugar and carry out the carbon source optimizing experiment, 7 kinds of steamed bun stuffed with sugars have been drawn together sucrose, mannose, lactose, raffinose, starch, fructose and glucose.They are added to the water respectively to stir is configured as sugar juice, and the initial concentration of every kind of sugar juice is 30.0g/L.
Mix with 500mL distilled water and 10g mineralized waste, obtain being rich in the mixed bacteria liquid of methane oxidation microorganism.Sugar juice is added the consumption situation behind the investigation 70h in the mixed bacteria liquid according to 3.0g/L.Mixed bacteria liquid can determine that the sulfuric acid anthrone method is adopted in the detection of sugared concentration by the consumption concentration of sugar to the consumption situation of different sugar.The mixed bacteria liquid that will contain sugar is not contained the sugar juice of bacterium after centrifugal with centrifuge, get of the volumetric flask dilution of the above-mentioned sugar juice of 2.5mL with 50mL, getting 1mL places 10mL to have stopper and scale test tube, in test tube, add the 4mL anthrone reagent then respectively, accurately boil after 10 minutes and take out, with the running water cooling, place after about ten minutes under the room temperature at once, obtain the chromophoric solution of sugar, measure absorbance at the 635nm place.As shown in Figure 2,7 kinds of saccharide compounds can utilize by mixed bacterium liquid, and wherein raffinose consumes the most soon, illustrates that raffinose just has facilitation preferably to the growth of Mixed Microbes.
The absorbance of bacterium liquid can qualitative sign thalline concentration, the high more explanation thalline of absorbance growing way is good more.The employing spectrophotometer detects, and wavelength is 560nm.Glass syringe with 5mL extracts in the mixed bacteria liquid adding cuvette that has added sugar juice about 3mL, is reference with distilled water.Please see Figure 3, by the absorbance of bacterium liquid as can be known, raffinose and mannose are carbon source, and the thalline growing way is better.And sucrose, starch and glucose are that the carbon source growing way is general.The growing state of thalline has characterized methane oxidation microbial growth situation qualitatively, and the ability of methane biologic oxidation also needs to judge by the oxygenation efficiency to methane gas of reality, please see embodiment 2.
6 kinds of saccharide compounds have been chosen, comprising sucrose, mannose, lactose, raffinose, starch and fructose.They are added to the water respectively to stir are configured as sugar juice, the initial concentration of every kind of sugar juice is 1.0g/L, the 20g mineralized waste is equipped with in adding, and (landfill is more than 8 years, particle diameter is 0.5～4mm, moisture content is 20～30% stable mineralized waste) the sealing serum bottle in, and in bottle, pour methane, simulate the household refuse landfill sites actual conditions.Investigate the situation of its mineralized waste to methane oxidation.The mensuration of the carbon dioxide of methane and generation all adopts gas-chromatography in the gas phase: gas chromatograph is the GC-14B type of SHIMADZU company, thermal conductivity detector (TCD), stainless steel packed column, column length 2m; Carrier is GDX-104,80～100 orders; Carrier gas is a hydrogen, carrier gas flux 30mL/min, bridge electric current 120mA; Detector, injector and chromatogram column temperature are respectively: 90 ℃, 40 ℃, 40 ℃; Adopt the gas sampling needle sampler of 1mL, sample size is 0.2mL.The volume fraction of component converts by calibration curve and obtains, and the consumption of methane is used for characterizing the ability of mineralized waste degraded methane, and the growing amount of carbon dioxide is used for characterizing the metabolic characteristics of thalline in the mineralized waste.
By Fig. 4～Fig. 9 as can be known, add sugared mineralized waste and all possessed stronger methane consumption ability.Wherein, when mannose is carbon source the ability of mineralized waste methane degraded relatively a little less than, cultivated 30d after methane content reduce to about 10% (Fig. 4) by 30%, it is in the experimentation of carbon source (Fig. 5 and Fig. 6) that same result also occurs in raffinose and fructose.
As shown in Figure 7, sucrose be the mineralized waste of carbon source after having cultivated 15d, tangible decline has appearred in methane, and has realized the full consumption of methane in 1 week.Similar phenomena has also appearred in starch, and the rapid degraded of methane occurs in cultivates after the 10d, and after 10d in methane full consumption (Fig. 8).This has illustrated with sucrose and starch and has been the metabolic pathway of carbon source and is that the metabolic pathway of carbon source is very close with methane, it is hereby ensured that thalline when utilizing sugar, has also kept the activity with oxidizing of methylene associated biomolecule enzyme.In addition, the domestication (normal temperature is placed down) of adding the mineralized waste of sugar and substantially all needing 7～10 days just can be guaranteed the activity of oxidizing of methylene.Mineralized waste with interpolation starch is an example, and the methane oxidation rate is 810g/ (m through converting as can be known
2D), compare with the blank assay of sugared soln not, methane oxidation efficient exceeds about 3 times.
As shown in Figure 9, be the mineralized waste of the carbon source methane of can degrading well with the lactose, but find methane simultaneously in the process of degraded that carbon dioxide does not significantly increase, this has illustrated that methane mainly transforms for thalline.
Claims (3)
1. the method for a strengthening methane oxidation of covering layer of landfill site, it is characterized in that: at first, measure sugar according to the ratio that adds 0.5～3kg sugar in the mineralized waste per ton, then, 1～3 portion of quality sugar is added in 100 parts of quality water, stirs, add in the mineralized waste after being configured as sugar juice, mix, static at normal temperatures again 7～10 days, become covering layer material; At last, after the consumer waste filling and embedding unit process finishes, according to the conventional covering process of end cover layer 40-60cm thickness, with the even spreading of covering layer material on the house refuse surface; After testing, methane oxidation efficient reaches 810g/ (m
2D).
2. the method for a kind of strengthening methane oxidation of covering layer of landfill site according to claim 1 is characterized in that: described mineralized waste is a landfill more than 8 years, and particle diameter is 0.5～4mm, and moisture content is 20～30% mineralized waste.
3. the method for a kind of strengthening methane oxidation of covering layer of landfill site according to claim 1, it is characterized in that: described sugar is commercially available glucose, mannose, fructose, sucrose, raffinose, lactose or starch.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101966422A (en) * | 2010-09-26 | 2011-02-09 | 环境保护部南京环境科学研究所 | Method for processing CH4 in landfill site after acclimatizing and mineralizing garbage by utilizing percolate |
| CN102319716A (en) * | 2011-06-02 | 2012-01-18 | 福建工程学院 | Covering layer of sanitary landfill |
| CN105311953A (en) * | 2014-07-31 | 2016-02-10 | 中国农业大学 | Paddy soil, matured compost and active carbon compound methane biofilter material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1807331A (en) * | 2005-12-30 | 2006-07-26 | 同济大学 | Covering layer material based on sludge for daily garbage landfill |
| CN101062848A (en) * | 2007-04-30 | 2007-10-31 | 同济大学 | Methane oxidation covering material for life refuse landfill |
| CN101492642A (en) * | 2009-02-23 | 2009-07-29 | 同济大学 | Process for producing methyl hydride oxidized bacteria agent |
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- 2010-04-27 CN CN2010101574414A patent/CN101823072B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1807331A (en) * | 2005-12-30 | 2006-07-26 | 同济大学 | Covering layer material based on sludge for daily garbage landfill |
| CN101062848A (en) * | 2007-04-30 | 2007-10-31 | 同济大学 | Methane oxidation covering material for life refuse landfill |
| CN101492642A (en) * | 2009-02-23 | 2009-07-29 | 同济大学 | Process for producing methyl hydride oxidized bacteria agent |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101966422A (en) * | 2010-09-26 | 2011-02-09 | 环境保护部南京环境科学研究所 | Method for processing CH4 in landfill site after acclimatizing and mineralizing garbage by utilizing percolate |
| CN101966422B (en) * | 2010-09-26 | 2013-06-05 | 环境保护部南京环境科学研究所 | Method for processing CH4 in landfill site after acclimatizing and mineralizing garbage by utilizing percolate |
| CN102319716A (en) * | 2011-06-02 | 2012-01-18 | 福建工程学院 | Covering layer of sanitary landfill |
| CN102319716B (en) * | 2011-06-02 | 2013-02-13 | 福建工程学院 | Covering layer of sanitary landfill |
| CN105311953A (en) * | 2014-07-31 | 2016-02-10 | 中国农业大学 | Paddy soil, matured compost and active carbon compound methane biofilter material |
| CN105311953B (en) * | 2014-07-31 | 2017-10-27 | 中国农业大学 | Paddy soil, matured compost and the compound methane biofilter material of activated carbon |
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