CN101810659A - Total saponins of salvia chinensis benth, and medical application and preparation method thereof - Google Patents
Total saponins of salvia chinensis benth, and medical application and preparation method thereof Download PDFInfo
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Abstract
The invention relates to total saponins of salvia chinensis benth, and medical application and a preparation method thereof. The total saponins of the salvia chinensis benth is characterized in that the total saponins are rich in 20S-protopanoxadiol and 20S-protopanaxtriol ginseng saponin compounds.
Description
Technical field: the present invention relates to isolating extract from Herba Salviae Chinensis, particularly relate to isolating Herba Salviae Chinensis total saponins and preparation technology, medical usage and analytical method from Herba Salviae Chinensis with medical usage.
Background technology: Radix Ginseng (Panax ginseng C.A.Meyer) is the root of Araliaceae Radix Ginseng, beginning is stated from Shennong's Herbal, Radix Ginseng has the effect of " tonifying five ZANG-organs, peace spirit, decide that soul, spasmolytic are throbbed with fear, removed pathogen, happy Fructus Alpiniae Oxyphyllae makes eye bright; obey to make light of one's life by commiting suicide for a long time and prolong life ", enjoy the good reputation of " king of Chinese medicine ", occupy the hat of northeast three essentials-essence.Radix Ginseng has good preventing health care and therapeutic effect to multiple disease, is particularly having more unique effect aspect resisting fatigue, defying age, prophylaxis of cancer and the cardiovascular disease.Ginseng health product also produces thus.Ginseng health product in the market mostly is to join goods entirely, and in fact, in the Radix Ginseng there be than big difference the physiological function of each composition.The main active of Radix Ginseng is ginsenoside (ginsenoside), kind surplus the present ginsenoside who has separated and identified reaches 60, and various ginsenosides have its unique physiologically active.The experimental results shows: the ginsenoside has resisting fatigue, slow down aging, adjusting central nervous system, human body immunity improving power, improves the cardiovascular and cerebrovascular vessel blood supply insufficiency, suppresses effects such as growth of tumour cell.Therefore can predict, each monomeric series nutrition health care food of ginsenoside can be because of the remarkable single-minded health-product market that occupies 21 century of its effect.
It is worth noting simultaneously how Radix Ginseng brings into play optimal efficacy in vivo on earth? after people took Radix Ginseng and gen-seng, ginsenoside's class was finished metabolism overall processes such as decomposition, absorption, distribution in vivo and is brought into play effect.Human body enteral microorganism species formation has very big individual variation because of the people.Wherein, whether the microorganism of decomposing the ginsenoside exists how much also closely related with different body constitution, race, region, environment, life habit reach quantity.Infer that thus the key of taking the physical variation that produces behind Radix Ginseng and the preparation thereof is the different and absorption difference that causes of different human body enteral microbial decomposition ginsenoside ability, thus the ginsenoside be absorbed with individual variation, therefore also different at clinical effectiveness.In the early-stage Study, surprised discovery ginsenoside is hardly by (Rg that human body absorbs
11.9%, Rb
10.1%, Rb
23.7%), oral ginsenoside is not decomposed by gastric juice and liver enzyme.The ginsenoside has kind more than 30, is that the glucosides of parent is decomposed into its metabolite ((protopanaxatriol-FGM by enteric microorganism with Protopanaxatriol
4), be that the glucosides of parent is decomposed into its metabolite (protopanaxadiol-FGM by enteric microorganism with the protopanoxadiol
1).So it is not ginsenoside but by the metabolite of enteral microbial decomposition that Radix Ginseng is brought into play usefulness in vivo.Very urgent by the full ginseng goods that conversion technology exploitation high added value Radix Ginseng product replaces in the market.
Herba Salviae Chinensis is a kind of method that adopts fermentation, the fermented ginseng-ginsenoside metabolin FGM that can very absorb for human body in external preparation
1And FGM
4, its product safety, curative effect determine not have any toxicity, side effect.And fermented ginseng and enteral microbial decomposition ability are irrelevant, obtain with microbial fermentation it mainly contain ginsenoside's analyte protopanaxadiol and protopanaxatriol.The metabolite of directly taking the ginsenoside is avoided the individual variation of Radix Ginseng usefulness, has overcome this usefulness individual variation after being decomposed Radix Ginseng that ginsenoside's microorganism causes that absorption difference causes and taken by the human body enteral, has increased the usefulness of Radix Ginseng.Because Herba Salviae Chinensis is the metabolite that utilizes our human body beneficial's enteral microbial decomposition, be free from side effects, safe and reliable.
Total saponins and Radix Ginseng in the Herba Salviae Chinensis that the present invention finds to handle by fermentation compare, and significant chemical change has taken place its chemical composition, and therefore, the Herba Salviae Chinensis total saponins for preparing by the present invention is new Chinese medicine extract, and its chemical composition is analyzed through LC-MS.And find that it has significant resisting fatigue, slow down aging, adjusting central nervous system, human body immunity improving power, improves the cardiovascular and cerebrovascular vessel blood supply insufficiency, suppresses biological activitys such as growth of tumour cell, is expected to be applied to treat and prevent food, functional food and the medicine of functions such as antitumor, resisting fatigue, enhance immunity.
Summary of the invention:
1, purpose of the present invention: the invention provides a kind of extract, this extract contains the 20S-protopanoxadiol, 20S-Protopanaxatriol, 20S-ginsenoside Rh
2Aglycon, the 20S-ginsenoside Rh
1Aglycon, compound K aglycon and 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K etc. (seeing following table for details), its prominent features is for containing Radix Panacis Quinquefolii saponin L
14(structure sees the following form).20S-protopanoxadiol type saponin wherein and 20S-Protopanaxatriol type saponin sum content are more than 60%, and 20S-protopanoxadiol and 20S-Protopanaxatriol sum content are more than 2%.The weight content of the wherein each component of extract of the present invention sees the following form:
The outstanding feature of extract of the present invention is,, its prominent features is for containing Radix Panacis Quinquefolii saponin L
14, and the 20S-protopanoxadiol in the extract, 20S-Protopanaxatriol content are high more than 20 times than Radix Ginseng extract, and 20 configuration is natural 20S configuration (as follows).
Wherein R1 representative: hydrogen, glucose, perhaps rock algae disaccharidase
R2 representative: hydrogen, glucose, perhaps 2-rhamanopyranosyl glucose
R3 representative: hydrogen, glucose, 6-glucosyl group glucose, the Arabic glycosyl glucose of 6-furan type, the Arabic glycosyl glucose of 6-pyranoid form, 6-pyranoid form xylosyl glucose, xylose
Wherein: 20S-protopanoxadiol, 20S-Protopanaxatriol, 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, compound K, 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K, be known compound.
Compound K representative: 20S-protopanoxadiol-20-O-glucoside
The present invention also comprises preparation method of extract of the present invention, it is characterized in that, step is as follows: Herba Salviae Chinensis water or water-containing organic solvent extract, extract is used macroporous resin, ion exchange resin, polyamide, polydextran gel, reverse phase silica gel isochromatic spectrum means are refining separates, elder generation's water or low concentration organic solvent eluting are removed impurity, reuse high levels of organic solvents eluting obtains product, adopt above repeatedly chromatographic isolation to make with extra care purification, or recrystallization purifying, wherein, extraction is selected from ethanol with organic solvent, methanol, acetone, macroporous resin, ion exchange resin comprises styrene type, the acrylic type macromolecule resin.
Preferably, preparation method of the present invention, step is as follows: Herba Salviae Chinensis water reflux, extract.Reclaim under reduced pressure gets syrupy shape extractum.With suitable quantity of water suspendible, dissolving, successively with water, 10% ethanol, 70% ethanol, 95% ethanol elution, obtain water elution part, 10% ethanol elution part, 70% ethanol elution part, 95% ethanol elution part respectively through macroporous resin PD101 column chromatography.
Wherein, 70% ethanol elution partly is rich in Radix Panacis Quinquefolii saponin L
14, the 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K, 20S-ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rd, Ginsenoside Rc, ginsenoside Re, ginsenoside Rf, ginseng saponin F
1, the ginsenoside Ra
1, the ginsenoside Ra
3, the 20S-ginsenoside Ra
3Deng the triterpene saponin constituents, be preferred ingredient of the present invention, i.e. the preferred extract of the present invention.
Water elution part, 10% ethanol elution part, 95% ethanol elution part also belong to content of the present invention.The merging thing of each component also belongs to content of the present invention.
In addition, the present invention also provides the method for utilizing the Herba Salviae Chinensis crude extract acid hydrolysis contain The compounds of this invention to prepare Herba Salviae Chinensis extract of the present invention, and wherein said acid is selected from mineral acid and organic acid as sulphuric acid, hydrochloric acid, formic acid, acetic acid, trifluoroacetic acid.
The present invention also comprise chemical compound of the present invention in preparation resisting fatigue, slow down aging, adjusting central nervous system, human body immunity improving power, improve the cardiovascular and cerebrovascular vessel blood supply insufficiency, suppress growth of tumour cell, go greasy sober up, improve looks and the arteries and veins of invigorating blood circulation, defying age, blood fat reducing, cholesterol reducing, triglyceride reducing, improve blood viscosity and microcirculation, blood vessel dilating, removing free radical, strengthen body immunity, the application in the antitumor, food, functional food and medicine such as anticancer.
The present invention also comprises the detection method of above-mentioned Herba Salviae Chinensis total triterpene saponins extract: as: HPLC analyzes, and is detector with mass spectrum (MS) detection, ultraviolet (UV) detection, circular dichroism spectra (CD) detection, evaporative light scattering detector etc.
The configuration of reference substance solution: 20S-protopanoxadiol, 20S-Protopanaxatriol, 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, triterpenoid compound chemical reference substance precision such as compound K takes by weighing in right amount, is mixed with an amount of reference substance solution with methanol.
The configuration of sample solution: precision takes by weighing Herba Salviae Chinensis, each is an amount of for Herba Salviae Chinensis extract or Herba Salviae Chinensis total triterpene saponins sample, with 100ml chloroform (or methanol, ethyl acetate, acetone, acetonitrile, petroleum ether, organic solvent and solution in different concentration thereof such as cyclohexane extraction) extract, reclaim extracting solution, methanol (or chloroform, ethyl acetate, acetone, acetonitrile, petroleum ether, organic solvent and solution in different concentration thereof such as cyclohexane extraction) dissolving, the little chromatographic column pretreatment of SPE through filling ODS, 95% acetonitrile (or methanol, ethyl acetate, acetone, chloroform, petroleum ether, organic solvent and solution in different concentration thereof such as cyclohexane extraction) washing, merge eluent, methanol constant volume, filter, promptly.
Method of testing: carrying out HPLC and analyze, is detector with mass spectrum (MS) detection, ultraviolet (UV) detection, circular dichroism spectra (CD) detection, evaporative light scattering detector etc.With the chromatographic peak area of each chemical compound in the sample, corresponding chromatographic peak area with the standard control sample according to standard curve method (or 1 method of external standard, 2 methods of external standard etc.), carries out quantitative analysis, calculates, promptly.
The present invention also comprises the pharmaceutical composition that contains extract of the present invention.
The pharmaceutical preparation that can be Herba Salviae Chinensis extract of the present invention be mixed with the medicine acceptable carrier of described compositions.
These preparations comprise that general pharmaceutical dosage form is as tablet, capsule, granule, oral liquid, injection etc.
Pharmaceutical composition of the present invention is determined usage and dosage according to patient's situation in use, but obeys every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet, every dose of 1mg-1000mg.
Test shows, extract of the present invention has anti-tumor activity and human body immunity improving power activity, point out it to can be used for resisting fatigue, slow down aging, adjusting central nervous system, human body immunity improving power, improve the cardiovascular and cerebrovascular vessel blood supply insufficiency, suppress growth of tumour cell, go greasy sober up, improve looks and the arteries and veins of invigorating blood circulation, defying age, blood fat reducing, cholesterol reducing, triglyceride reducing, improve blood viscosity and microcirculation, blood vessel dilating, removing free radical, strengthen body immunity, the application of antitumor, medicine, functional food and food such as anticancer.
Below experiment is used to illustrate that Herba Salviae Chinensis total triterpene saponins extract of the present invention is more superior than existing analog.
Toxicity test:
Medicine: the Herba Salviae Chinensis total triterpene saponins (70% ethanol elution position) of the embodiment of the invention 2 methods preparation,
Reference substance: Radix Ginseng total triterpene saponins
The mice of Herba Salviae Chinensis total triterpene saponins of the present invention is oral once to be given and maximum administration concentration 10g/Kg body weight, there is no toxicity, and blood parameters is all normal,
Matched group: the mice of Herba Salviae Chinensis total triterpene saponins of the present invention is oral once to be given and maximum administration concentration 10g/Kg body weight, dissects corpse and sees liver redness, abnormal liver function.
Therefore illustrate that drug toxicity of the present invention is less than matched group.
The test of pesticide effectiveness:
Medicine: the Herba Salviae Chinensis total triterpene saponins (70% ethanol elution position, ZSZ represents) of the embodiment of the invention 2 methods preparation
Reference substance: Radix Ginseng total triterpene saponins
Anti-fatigue test: prolong mice heavy burden (body weight 5%) swimming time result of the test and show: the Herba Salviae Chinensis total triterpene saponins can be suitable with the anti-fatigue active of Radix Ginseng total saponins in the 50mg/Kg body weight, illustrates that its activity is stronger.
| Group | Dosage mg/Kg body weight | Number of animals | Swimming time min |
| Model group | ??10 | ??10.48±2.61 | |
| The ZSZ low dose group | ??25 | ??10 | ??16.11±3.49 ** |
| Dosage group among the ZSZ | ??50 | ??10 | ??33.18±2.80 ** |
| The ZSZ high dose group | ??100 | ??10 | ??36.93±3.47 ** |
| Positive drug | ??250 | ??10 | ??35.66±3.82 ** |
Compare with model group
*P＜0.05,
*P＜0.01
Hypoxia endurance test: the mice normal pressure is airtight hypoxia endurance time result show: the Herba Salviae Chinensis total triterpene saponins can be quite active with the anoxia enduring of Radix Ginseng total saponins in the 50mg/Kg body weight, illustrates that its activity is stronger.
| Group | Dosage mg/Kg body weight | Number of animals | Time-to-live min |
| Model group | ??10 | ??33.21±5.62 | |
| The ZSZ low dose group | ?25 | ??10 | ??39.60±6.03 * |
| Dosage group among the ZSZ | ?50 | ??10 | ??47.34±4.98 ** |
| The ZSZ high dose group | ?100 | ??10 | ??48.51±6.11 ** |
| Positive drug | ?250 | ??10 | ??45.64±5.73 ** |
Compare with model group
*P＜0.05,
*P＜0.01
The antitumor test: the result that influences to Hela tumor-bearing mice tumor bulk-growth shows: the Herba Salviae Chinensis total triterpene saponins can be suitable with the anti-tumor activity of Radix Ginseng total saponins in the 50mg/Kg body weight, illustrates that its activity is stronger.
| Group | Dosage mg/Kg body weight | Number of animals | The heavy g of tumor | Tumour inhibiting rate % |
| Model group | ??10 | ??1.032±0.561 | ||
| The ZSZ low dose group | ??25 | ??10 | ??0.611±0.303 * | ??40.8 |
| Dosage group among the ZSZ | ??50 | ??10 | ??0.491±0.210 ** | ??52.4 |
| The ZSZ high dose group | ??100 | ??10 | ??0.485±0.214 ** | ??53.0 |
| Positive drug | ?250 | ??10 | ??0.587±0.209 * | ??43.1 |
Compare with model group
*P＜0.05,
*P＜0.01
The specific embodiment:
1, extraction separation prepares the method for above triterpene aglycon and saponins compound and Herba Salviae Chinensis total triterpene saponins from Herba Salviae Chinensis.Its key technology is that water or water-containing organic solvent extract, extract is used macroporous resin, ion exchange resin, polyamide, polydextran gel, reverse phase silica gel isochromatic spectrum means are refining separates, elder generation's water or low concentration organic solvent eluting are removed impurity, reuse high levels of organic solvents eluting obtains product, adopt above repeatedly chromatographic isolation to make with extra care purification, or recrystallization purifying.Wherein, extraction comprises ethanol, methanol, acetone etc. with organic solvent.Macroporous resin, ion exchange resin comprise macromolecule resins such as various styrene types, acrylic type.
2, utilize the Herba Salviae Chinensis extract contain triterpene aglycon and saponins compound, invented the method that acid hydrolysis prepares Herba Salviae Chinensis hydrolyzing saponin or triterpene aglycon.Used acid comprises the ore deposit acid and the organic acid of various concentration, ore deposit acid as: sulphuric acid, hydrochloric acid etc., organic acid such as formic acid, acetic acid, trifluoroacetic acid etc.
3, find the Herba Salviae Chinensis total triterpene saponins resisting fatigue, slow down aging, adjusting central nervous system, human body immunity improving power, improve the cardiovascular and cerebrovascular vessel blood supply insufficiency, suppress growth of tumour cell, go greasy sober up, improve looks and the arteries and veins of invigorating blood circulation, defying age, blood fat reducing, cholesterol reducing, triglyceride reducing, improve blood viscosity and microcirculation, blood vessel dilating, removing free radical, strengthen body immunity, antitumor, medical usage such as anticancer.
4, set up analytical method: HPLC analyzes, and is detector with mass spectrum (MS) detection, ultraviolet (UV) detection, circular dichroism spectra (CD) detection, evaporative light scattering detector etc.
The configuration of reference substance solution: the configuration of reference substance solution: 20S-protopanoxadiol, 20S-Protopanaxatriol, 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, triterpenoid compound chemical reference substance precision such as compound K takes by weighing in right amount, is mixed with an amount of reference substance solution with methanol.
The configuration of sample solution: precision takes by weighing the total triterpene sample of Herba Salviae Chinensis, and each is an amount of, extracts with 100ml chloroform (or organic solvent and solution in different concentration thereof such as methanol, ethyl acetate, acetone, acetonitrile, petroleum ether, cyclohexane extraction), reclaims extracting solution, methanol
(or organic solvent and solution in different concentration thereof such as chloroform, ethyl acetate, acetone, acetonitrile, petroleum ether, cyclohexane extraction) dissolving, the little chromatographic column pretreatment of SPE through filling ODS, 95% acetonitrile (or organic solvent and solution in different concentration thereof such as methanol, ethyl acetate, acetone, chloroform, petroleum ether, cyclohexane extraction) washing, merge eluent, methanol constant volume, filter, promptly.
Method of testing: carrying out HPLC and analyze, is detector with mass spectrum (MS) detection, ultraviolet (UV) detection, circular dichroism spectra (CD) detection, evaporative light scattering detector etc.With the chromatographic peak area of each chemical compound in the sample, corresponding chromatographic peak area with the standard control sample according to standard curve method (or 1 method of external standard, 2 methods of external standard etc.), carries out quantitative analysis, calculates, promptly.
Embodiment 1: the extraction of triterpene saponin componds preparation in the Herba Salviae Chinensis total triterpene saponins
Herba Salviae Chinensis 6kg is with twice of 95% ethanol 60L reflux, extract.Decompression recycling ethanol gets syrupy shape extractum.Use the suitable quantity of water suspendible, use petroleum ether, chloroform, ethyl acetate, n-butanol extraction successively.
Petroleum ether extraction layer (180g) is through silica gel column chromatography, with petroleum ether/acetone gradient elution, obtain chemical compound 20S-protopanoxadiol, 20S-Protopanaxatriol, 20S-ginsenoside Rh through separation means of purification such as silica gel column chromatography repeatedly, preparation thin layer, Sephadex LH-20 column chromatographies again
2
Chloroform extraction layer (30g) is through silica gel column chromatography repeatedly, with petroleum ether/acetone gradient elution, through silica gel column chromatography, preparation thin layer, Sephadex LH-20 column chromatography five equilibrium separate from means of purification and obtain chemical compound 20S-ginsenoside Rh repeatedly
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K.
Ethyl acetate extraction layer (85g) is through silica gel column chromatography repeatedly, with petroleum ether/acetone gradient elution, and the preparation thin layer, separation such as SephadexLH-20 obtain chemical compound 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K.
N-butanol extraction layer (640g) successively with water, 30% ethanol, 70% ethanol, 95% ethanol elution, obtains water layer, 30% alcohol layer, 70% alcohol layer, 95% alcohol layer (seeing Fig.5) through macroporous resin PD101 column chromatography respectively.Get 70% ethanol elution part 100g, through silica gel column chromatography, with the chloroform/methanol gradient elution, again through Sephadex LH-20 column chromatography, prepare HPLC etc. repeatedly and separate means of purification, separate obtaining chemical compound 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K, 20S-ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rd, Ginsenoside Rc, ginsenoside Re, ginsenoside Rf, ginseng saponin F
1, the ginsenoside Ra
1, the ginsenoside Ra
3, the 20S-ginsenoside Ra
3
Embodiment 2: the extraction preparation of Herba Salviae Chinensis total triterpene saponins
Herba Salviae Chinensis 6kg, twice of water 60L reflux, extract.Reclaim under reduced pressure gets syrupy shape extractum.With suitable quantity of water suspendible, dissolving, successively with water, 30% ethanol, 70% ethanol, 95% ethanol elution, obtain water elution part, 10% ethanol elution part, 70% ethanol elution part, 95% ethanol elution part respectively through macroporous resin PD101 column chromatography.Wherein, 70% ethanol elution partly is rich in the 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K, 20S-ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rd, Ginsenoside Rc, ginsenoside Re, ginsenoside Rf, ginseng saponin F
1, the ginsenoside Ra
1, the ginsenoside Ra
3, the 20S-ginsenoside Ra
3Deng the triterpene saponin constituents, total saponin content reaches more than 80%.95% ethanol elution part and 10% ethanol elution part also contain the triterpene saponin constituents, and its content is few low, but its composition is different with 70% ethanol elution part with ratio, and therefore, above extract method all can be used for the preparation of Herba Salviae Chinensis total triterpene saponins.
Embodiment 3: the HPLC-TOF-MS of Herba Salviae Chinensis total triterpene saponins analyzes
The configuration of reference substance solution: triterpenoid compound chemical reference substance precision takes by weighing in right amount, is mixed with an amount of reference substance solution with methanol.
The configuration of sample solution: precision takes by weighing the total triterpene sample of Herba Salviae Chinensis, and each is an amount of, with 100ml chloroform (or methanol, ethyl acetate, acetone, acetonitrile, petroleum ether, organic solvent and solution in different concentration thereof such as cyclohexane extraction) extract, reclaim extracting solution, methanol (or chloroform, ethyl acetate, acetone, acetonitrile, petroleum ether, organic solvent and solution in different concentration thereof such as cyclohexane extraction) dissolving, the little chromatographic column pretreatment of SPE through filling ODS, 95% acetonitrile (or methanol, ethyl acetate, acetone, chloroform, petroleum ether, organic solvent and solution in different concentration thereof such as cyclohexane extraction) washing, merge eluent, methanol constant volume, filter, promptly.
Method of testing: carrying out HPLC and analyze, is detector with mass spectrum (MS) detection, ultraviolet (UV) detection, circular dichroism spectra (CD) detection, evaporative light scattering detector etc.With the chromatographic peak area of each chemical compound in the sample, corresponding chromatographic peak area with the standard control sample according to standard curve method (or 1 method of external standard, 2 methods of external standard etc.), carries out quantitative analysis, calculates, promptly.
Utilize 20S-protopanoxadiol, 20S-Protopanaxatriol, 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K, 20S-ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rd, Ginsenoside Rc, ginsenoside Re, ginsenoside Rf, ginseng saponin F
1, the ginsenoside Ra
1, the ginsenoside Ra
3, the 20S-ginsenoside Ra
3Accurate molecular weight, analyze, qualitative, determine chemical constituent and content thereof in the Herba Salviae Chinensis total saponins quantitatively.
Embodiment 4: acid hydrolysis
Get the sample of 1mg respectively, be dissolved in the ampoule bottle, envelope bottle, 110 ℃ of reaction 2h with 1ml 2N trifluoroacetic acid (TFA).CHCl
3Extract water layer N three times
2Dry up, add methanol, reuse N
2Dry up, there is not TFA in this process in product repeatedly, promptly.
Claims (10)
1. a Herba Salviae Chinensis total triterpene saponins class extract is characterized in that described extract contains 20S-protopanoxadiol, 20S-Protopanaxatriol, 20S-ginsenoside Rh
2Aglycon, 20S-ginsenoside Rh
1Aglycon, compound K aglycon and 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K, its prominent features is for containing Radix Panacis Quinquefolii saponin L
14(structure sees the following form).20S-protopanoxadiol type saponin wherein and 20S-Protopanaxatriol type saponin sum content are more than 60%, and 20S-protopanoxadiol and 20S-Protopanaxatriol sum content are more than 2%.
2. the Herba Salviae Chinensis total triterpene saponins class preparation method of extract of claim 1, it is characterized in that, step is as follows: Herba Salviae Chinensis water or water-containing organic solvent extract, extract is crossed macroporous resin, ion exchange resin, polyamide, polydextran gel or reverse phase silica gel chromatographic column, elder generation's water or low concentration organic solvent eluting are removed impurity, reuse high levels of organic solvents eluting obtains product, and is refining with chromatographic isolation.
3. by the described preparation method of claim 2, it is characterized in that wherein, extract used organic solvent and be selected from ethanol, methanol or acetone, macroporous resin, ion exchange resin comprise styrene type, acrylic type macromolecule resin.
4. by the described preparation method of claim 2, it is characterized in that step is as follows: Herba Salviae Chinensis 6kg, twice of water 60L reflux, extract.Reclaim under reduced pressure gets syrupy shape extractum.With suitable quantity of water suspendible, dissolving, successively with water, 30% ethanol, 70% ethanol, 95% ethanol elution, obtain water elution part, 10% ethanol elution part, 70% ethanol elution part, 95% ethanol elution part respectively through macroporous resin PD101 column chromatography.Wherein, 70% ethanol elution partly is rich in the 20S-ginsenoside Rh
2, the 20S-ginsenoside Rh
1, the ginsenoside Rg
1, the 20S-ginsenoside Rg
3, compound K, 20S-ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rd, Ginsenoside Rc, ginsenoside Re, ginsenoside Rf, ginseng saponin F
1, the ginsenoside Ra
1, the ginsenoside Ra
3, the 20S-ginsenoside Ra
3Deng the triterpene saponin constituents.
5. the Herba Salviae Chinensis total triterpene saponins class preparation method of extract of claim 1, it is characterized in that, step is as follows: utilize the acid hydrolysis preparation that contains Herba Salviae Chinensis total triterpene saponins class extract in the claim 1, described acid is selected from sulphuric acid, hydrochloric acid, formic acid, acetic acid, trifluoroacetic acid.
6. the Herba Salviae Chinensis total triterpene saponins class extract of claim 1 is in the preparation resisting fatigue, slow down aging, regulate the central nervous system, human body immunity improving power, improve the cardiovascular and cerebrovascular vessel blood supply insufficiency, suppress growth of tumour cell, go greasy sobering up, improve looks and the arteries and veins of invigorating blood circulation, defying age, blood fat reducing, cholesterol reducing, triglyceride reducing, improve blood viscosity and microcirculation, blood vessel dilating, remove free radical, strengthen body immunity, antitumor, cancer therapy drug, functional food or Application in Food.
7. the pharmaceutical composition that contains the Herba Salviae Chinensis total triterpene saponins class extract of claim 1.
8. by the pharmaceutical composition of claim 7, it is characterized in that, contain the medicine acceptable carrier.
9. by the pharmaceutical composition of claim 7, it is characterized in that, comprise any pharmaceutically useful dosage form, as tablet, capsule, granule, oral liquid, injection.
10. by the pharmaceutical composition of claim 7, it is characterized in that, according to patient's situation, determine usage and dosage in use, but obey every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet, every dose of 1mg-1000mg.
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