CN101806746A - Rapid testing method of zinc salt in milk - Google Patents
Rapid testing method of zinc salt in milk Download PDFInfo
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- CN101806746A CN101806746A CN 201010150930 CN201010150930A CN101806746A CN 101806746 A CN101806746 A CN 101806746A CN 201010150930 CN201010150930 CN 201010150930 CN 201010150930 A CN201010150930 A CN 201010150930A CN 101806746 A CN101806746 A CN 101806746A
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Abstract
The invention relates to a rapid testing method of a zinc salt in milk, which comprises the following steps that: 2ml of a standard milk sample and 2ml of a milk sample to be tested are respectively added into 1ml of dithizone-carbon tetrachloride solution with the concentration of 0.1g/L; the standard milk sample is mixed with the dithizone-carbon tetrachloride solution uniformly to prepare a mixture A, and the color liquid surface dots of the mixture A and the color of lower layered liquid serve as standard color disks; the milk sample to be tested is mixed with the dithizone-carbon tetrachloride solution uniformly to prepare a mixture B, the color of liquid surface dots of the mixture B and the color of the lower layered liquid are observed; the color of the liquid surface dots of the mixture B and the color of the lower layered liquid are visually compared with the color of the standard color disks, if the color of the liquid surface dots is the same with the color of the lower layered liquid, the zinc content of the milk sample to be tested is above 50mg/kg, and if the color of the liquid surface dots is different from the color of the lower layered liquid, the zinc content of the milk sample to be tested is below 50mg/kg. The method has the advantages of low cost, convenient and rapid operation and short testing time, and is a rapid qualitative testing method.
Description
Technical field
The present invention relates to a kind of Ruzhong and mingle the detection method of composition, particularly a kind of Ruzhong zinc salt is mingled method for quick, belongs to the technical field that chemical analysis detects.
Background technology
Purchase is during fresh milk, and some illegal retailers can add zinc salt in milk, improves some physical and chemical index of milk with the means of deception, in order to guarantee to purchase the quality of fresh milk, is necessary when purchase a large amount of fresh milks to be tested.Yet it is limited to be used for detecting the method that the milk zinc salt mingles at present, mostly need be, and testing procedure is loaded down with trivial details by various instruments, and operability is very poor when a large amount of fresh milks purchase, can not practical requirement.
" the dithizone carbon tetrachloride method is directly measured urine zinc " (Liu Hanfang, " Chinese sanitary inspection magazine ", 2008 the 7th phases 18 volume, a kind of method that detects zinc salt in the solution is disclosed the 1302-1303 page or leaf), but this method of testing is comparatively loaded down with trivial details, also to add auxiliary reagent during use, prevent other material interference detection results, the fast detecting when it is unsuitable for purchasing milk.
In addition, the method for testing about zinc content in the food in the national standard is a quantitative test, the simple detection when also being not suitable for the purchase of a large amount of fresh milks.Press for and a kind ofly can differentiate the method that the fresh milk zinc salt is mingled simply, fast,
Summary of the invention
The method for quick that the object of the present invention is to provide a kind of Ruzhong zinc salt to mingle, it is with low cost, easy to operate fast, and phenomenon is obvious, highly sensitive, a large amount of in the time of can satisfying dairy produce manufacturing enterprise purchase fresh milk, the preliminary screening requirement of test fast.
The method for quick of a kind of Ruzhong of the present invention zinc salt comprises the steps:
(1) gets fat corrected milk(FCM) sample 2ml and newborn sample 2ml to be measured, add dithizone-carbon tetrachloride solution that 1ml concentration is 0.1g/L respectively;
(2) described fat corrected milk(FCM) sample being mixed with described dithizone-carbon tetrachloride solution, obtain potpourri A behind the mixing, is Standard colour board with the color of described potpourri A liquid level round dot and lower floor's liquid thereof;
(3) described newborn sample to be measured is mixed with described dithizone-carbon tetrachloride solution, obtain potpourri B behind the mixing, observe the color of potpourri B liquid level round dot and lower floor's liquid thereof;
(4) color and the range estimation of described Standard colour board of potpourri B liquid level round dot described in the step (3) and lower floor's liquid are compared, if color is identical, then described newborn sample zinc content to be measured is less than 50mg/kg, if the color difference, then described newborn sample zinc content to be measured is more than 50mg/kg.
In another embodiment of the present invention, zinc content is 50mg/kg in the described fat corrected milk(FCM) sample.
In also a kind of embodiment of the present invention, the preparation steps of described step dithizone-carbon tetrachloride solution is:
Take by weighing 0.01g dithizone solid reagent in small beaker, add phenixin, divide and dissolve institute for several times
The dithizone solid reagent of stating obtains solution C;
Described solution C is shifted the volumetric flask of putting into 100ml;
With phenixin solution C is settled to 100ml.
In another embodiment of the present invention, in described step (2) and the described step (3), after adding described dithizone-carbon tetrachloride solution mixing in described fat corrected milk(FCM) sample and the described newborn sample to be measured, the stand-by period of observing the described color of described potpourri A and described potpourri B is 1min～5min.
Dithizone-carbon tetrachloride solution is green, and water insoluble and density is less than water, with dithizone-carbon tetrachloride solution with after emulsion is mixed, dithizone-carbon tetrachloride solution and creaming of emulsion, and be positioned at above the emulsion.Because the dithizone in dithizone-carbon tetrachloride solution can generate red chelate with the zinc reaction in the emulsion, and phenixin can come out the chelate extraction of redness, therefore, obvious color can take place the dithizone-carbon tetrachloride solution that is positioned at the emulsion top changes, and promptly becomes redness by green.
For the fat corrected milk(FCM) sample that uses among the present invention, wherein zinc content is 50mg/kg, the dithizone and the zinc that add fully react, and guarantee that the dithizone total overall reaction that adds generates red chelate, and be extracted in the phenixin, at this moment, the dithizone-carbon tetrachloride solution on emulsion upper strata becomes redness by green.In newborn sample to be measured, when zinc content surpasses 50mg/kg, the dithizone that adds same amount, all dithizone total overall reactions generate red chelate, and the chelate quantity that generates in the chelate quantity that generates and the fat corrected milk(FCM) sample is identical, and be extracted in the phenixin, therefore, the color of the dithizone on emulsion to be measured upper strata-phenixin mixed liquor color and fat corrected milk(FCM) sample upper strata dithizone-phenixin mixed liquor is identical.In newborn sample to be measured, zinc content is during less than 50mg/kg, the dithizone that adds same amount, the dithizone partial reaction, generate red chelate, and be extracted in the phenixin, and the red chelate quantity that generates is less than the chelate quantity that generates in the fat corrected milk(FCM) sample, also have remaining green dithizone to remain in the phenixin simultaneously, after the chelate of redness mixed, the color of the dithizone-phenixin mixed liquor on newborn sample to be measured upper strata and the dithizone on fat corrected milk(FCM) sample upper strata-phenixin mixed liquor color produced obviously difference.
The method for quick that a kind of Ruzhong of the present invention zinc salt is mingled has following advantage:
1) with low cost, the real-time online quality monitoring when being suitable for the purchase of a large amount of fresh milks;
2) convenient to operation, proving time is short.The check time spent of single sample is about 1～2 minute, and is very efficient and convenient;
3) detecting when being limited to 50mg/kg of zinc content, detected artifacts is obvious, is convenient to visual observations;
4) shield the interference of other materials to test, accuracy height;
5) detect good reproducibility.
Embodiment
Below in conjunction with specific embodiments method of the present invention is done more detailed description, thereby the practicality and the operability of checking this method are got rid of the material that may cause interference to this method simultaneously by experiment.It will be appreciated by those skilled in the art that following embodiment all is used for the present invention's scope required for protection is carried out the description of exemplary, summarize the relative scope of each parameter of the present invention, thereby it can not be interpreted as a kind of concrete restriction of the present invention with this.
Embodiment 1
Material:
18 * 180 test tubes, 10ml calibrated pipet, 1ml calibrated pipet, weighing scoop, 100ml volumetric flask.
Preparation concentration is the dithizone carbon tetrachloride solution of 0.1g/l:
Accurately take by weighing the 0.01g dithizone in small beaker, add phenixin and divide several to be dissolved in the 100ml volumetric flask, and be settled to scale.
Preparation standard breast sample:
Get the newborn sample of known zinc content, the zinc content in the newborn sample is increased to 50mg/kg, make the fat corrected milk(FCM) sample by the mode of mending zinc salt.
Measure:
Get fat corrected milk(FCM) sample 2ml in test tube, mixing behind the dithizone carbon tetrachloride solution that adding 1ml configures leaves standstill after 1～2 minute and observes color.
The to be measured newborn sample 2ml that gets the need primary dcreening operation is in test tube, and mixing behind the dithizone carbon tetrachloride solution that adding 1ml configures leaves standstill after 1～2 minute and observes.
Judge according to following table:
| Milky juice face round dot to be measured and lower floor's color | Zinc salt content |
| Identical with fat corrected milk(FCM) sample test tube liquid level round dot with lower floor's color | ??≥50mg/kg |
| Different with fat corrected milk(FCM) sample test tube liquid level round dot with lower floor's color | ??＜50mg/kg |
The color of indication is identical herein is not meant that the absolute color on the physical significance is identical, but the color in the human eye vision error range is identical.
Method validation:
(1) sampling amount checking
| Sample number into spectrum | Sampling amount (ml) | Breast sample test tube liquid level round dot and lower floor's color |
| ??1 | ??1.0 | Medicine and sample mix, not layering |
| ??2 | ??2.0 | There is green round dot in liquid level central authorities |
| ??3 | ??3.0 | There is green round dot in liquid level central authorities, but not too obvious |
| ??4 | ??4.0 | There is green round dot in liquid level central authorities, but not too obvious, and are difficult for mixing |
| ??5 | ??5.0 | There is green round dot in liquid level central authorities, but not too obvious, and are difficult for mixing |
The sampling amount scope is 1～5ml, but by last table as can be seen, when being sampled as 2ml, the change color of newborn sample is the most obvious, operation when being convenient to detect and judgement.
(2) checking of dithizone phenixin concentration;
| Sample number into spectrum | Dithizone phenixin concentration (g/l) | Breast sample test tube liquid level round dot and lower floor's color |
| ??1 | ??0.05 | Medicine is sunken to the test tube bottom |
| ??2 | ??0.1 | There is green round dot in liquid level central authorities |
| Sample number into spectrum | Dithizone phenixin concentration (g/l) | Breast sample test tube liquid level round dot and lower floor's color |
| ??3 | ??0.2 | There is green round dot in liquid level central authorities |
| ??4 | ??0.3 | There is green round dot in liquid level central authorities |
| ??5 | ??0.4 | There is green round dot in liquid level central authorities |
| ??6 | ??0.5 | There is green round dot in liquid level central authorities |
| ??7 | ??1.0 | There is green round dot in liquid level central authorities |
Dithizone phenixin concentration range is 0.1～1g/l, and by checking, preferred 0.1g/l detects, and reduces inspection cost.
(3) checking of samples of latex and dithizone phenixin mixeding liquid volume ratio
In order to guarantee that in the fat corrected milk(FCM) sample, zinc and dithizone fully react, in zinc and dithizone reaction equation, the relationship between quality of zinc and dithizone, in the newborn sample that can settle the standard, samples of latex is 2: 1 with dithizone phenixin mixeding liquid volume ratio.In order to contrast newborn sample to be measured and fat corrected milk(FCM) sample its liquid level round dot or lower floor's color in test tube separately, in the newborn sample to be measured, newborn sample to be measured and dithizone phenixin mixeding liquid volume are than also being decided to be 2: 1.
(4) leave standstill the checking in reaction time
| Sample number into spectrum | Time of repose (min) | Breast sample test tube liquid level round dot and lower floor's color |
| ??1 | ??1 | Sample liquid mixes with medicine, is layering |
| ??2 | ??2 | There is green round dot in liquid level central authorities |
| ??3 | ??3 | There is green round dot in liquid level central authorities |
| ??4 | ??4 | There is green round dot in liquid level central authorities |
| ??5 | ??5 | There is green round dot in liquid level central authorities |
| ??6 | ??6 | The green round dot of liquid level central authorities shoals, and is difficult for observing and judges |
It is 1-5min that sample liquid leaves standstill reaction time range, and by checking, preferred 2min judges with interior, shortens proving time.
(5) the emulsion pH value is to the checking of testing result influence
To the pH value scope is that the fat corrected milk(FCM) sample of 5-10 detects, and testing result sees the following form:
By checking, newborn sample pH value can not have any impact to the testing result of zinc salt content.
(6) zinc salt is incorporated into the detection of carrying out physical and chemical index in the raw material milk by different proportion, variation in various degree appears in physical and chemical index, and is totally in rising trend, and wherein dry index rising variation is more obvious, sees the following form:
(7) (so-called interfering material is meant that common some in Ruzhong mix material to the material that respectively hydrogen peroxide, melamine, ammonium sulfate, barium sulphate, magnesium sulphate, ferrous sulphate, sodium bisulfite and manganese sulfate etc. may detect to take place be disturbed the Ruzhong zinc salt, these materials may react with dithizone equally, generate red chelate, thereby cause the testing result higher) add in proportion in the raw milk (known zinc content is less than 50mg/kg) that is up to the standards, the preparation sample, testing result sees the following form:
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Hydrogen peroxide | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Hydrogen peroxide | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Hydrogen peroxide | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Hydrogen peroxide | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Hydrogen peroxide | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Hydrogen peroxide | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Hydrogen peroxide | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Hydrogen peroxide | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Melamine | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Melamine | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Melamine | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Melamine | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Melamine | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Melamine | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Melamine | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Melamine | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Ammonium sulfate | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ammonium sulfate | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ammonium sulfate | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ammonium sulfate | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ammonium sulfate | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ammonium sulfate | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ammonium sulfate | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ammonium sulfate | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Barium sulphate | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Barium sulphate | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Barium sulphate | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Barium sulphate | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Barium sulphate | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Barium sulphate | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Barium sulphate | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Barium sulphate | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Magnesium sulphate | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Magnesium sulphate | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Magnesium sulphate | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Magnesium sulphate | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Magnesium sulphate | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Magnesium sulphate | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Magnesium sulphate | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Magnesium sulphate | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Ferrous sulphate | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ferrous sulphate | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ferrous sulphate | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Ferrous sulphate | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ferrous sulphate | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ferrous sulphate | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ferrous sulphate | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Ferrous sulphate | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Sodium bisulfite | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Sodium bisulfite | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Sodium bisulfite | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Sodium bisulfite | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Sodium bisulfite | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Sodium bisulfite | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Sodium bisulfite | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Sodium bisulfite | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Manganese sulfate | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Manganese sulfate | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Manganese sulfate | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Manganese sulfate | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Manganese sulfate | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Manganese sulfate | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Manganese sulfate | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Manganese sulfate | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Plumbi nitras | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Plumbi nitras | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Plumbi nitras | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Plumbi nitras | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Plumbi nitras | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Plumbi nitras | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Plumbi nitras | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Plumbi nitras | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Mix material | Mix content of material | Breast sample test tube liquid level round dot and lower floor's color | Zinc content detection result |
| Caddy | ??20mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Caddy | ??30mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Caddy | ??40mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Caddy | ??50mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Caddy | ??60mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Caddy | ??70mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Caddy | ??80mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
| Caddy | ??90mg/kg | There is green round dot in liquid level central authorities, and lower floor is green | ??＜50mg/kg |
This shows above-mentioned may the interfering material that the Ruzhong zinc salt detects can not being exerted an influence to the detection of zinc salt.
(8) replica test
8 raw material milk samples are carried out the detection of secondary parallel experiment respectively, and wherein sample 1,2, and 3 and 4 is the sample of known zinc content 〉=50mg/kg, sample 5,6, and 7 and 8 is the sample of known zinc content＜50mg/kg, concrete testing result sees the following form:
This shows that the testing result unanimity of this method, repeatability are better.
Claims (4)
1. the method for quick of a Ruzhong zinc salt comprises the steps:
(1) gets fat corrected milk(FCM) sample 2ml and newborn sample 2ml to be measured, add dithizone-carbon tetrachloride solution that 1ml concentration is 0.1g/L respectively;
(2) described fat corrected milk(FCM) sample being mixed with described dithizone-carbon tetrachloride solution, obtain potpourri A behind the mixing, is Standard colour board with the liquid level round dot of described potpourri A and the color of lower floor's liquid thereof;
(3) described newborn sample to be measured is mixed with described dithizone-carbon tetrachloride solution, obtain potpourri B behind the mixing, observe the liquid level round dot of potpourri B and the color of lower floor's liquid thereof;
(4) color and the range estimation of described Standard colour board of potpourri B liquid level round dot described in the step (3) and lower floor's liquid thereof are compared, if the color of described liquid level round dot and lower floor's liquid thereof is identical, then described newborn sample zinc content to be measured is more than 50mg/kg, if the color of described liquid level round dot and lower floor's liquid thereof is different, then described newborn sample zinc content to be measured is less than 50mg/kg.
2. detection method as claimed in claim 1, the zinc content in the wherein said fat corrected milk(FCM) sample is 50mg/kg.
3. detection method as claimed in claim 1, the preparation steps of wherein said dithizone-carbon tetrachloride solution is:
Take by weighing 0.01g dithizone solid reagent in small beaker, add phenixin, divide and dissolve described dithizone solid reagent for several times, obtain solution C;
Described solution C is shifted the volumetric flask of putting into 100ml;
With phenixin described solution C is settled to 100ml.
4. detection method as claimed in claim 1, wherein: in described step (2) and the described step (3), after adding described dithizone-carbon tetrachloride solution mixing in described fat corrected milk(FCM) sample and the described newborn sample to be measured, the stand-by period of observing the described color of described potpourri A and described potpourri B is 1 minute～5 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010150930 CN101806746A (en) | 2010-04-20 | 2010-04-20 | Rapid testing method of zinc salt in milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010150930 CN101806746A (en) | 2010-04-20 | 2010-04-20 | Rapid testing method of zinc salt in milk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101806746A true CN101806746A (en) | 2010-08-18 |
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| CN 201010150930 Pending CN101806746A (en) | 2010-04-20 | 2010-04-20 | Rapid testing method of zinc salt in milk |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102478570A (en) * | 2010-11-25 | 2012-05-30 | 内蒙古蒙牛乳业（集团）股份有限公司 | Qualitative detection method for detecting catalase in raw milk |
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| CN1595119A (en) * | 2004-07-06 | 2005-03-16 | 陕西科技大学 | Method for rapid detection of ammonium salts in fresh milk |
| CN101290323A (en) * | 2008-05-20 | 2008-10-22 | 内蒙古蒙牛乳业（集团）股份有限公司 | Cow milk and milk powder nitrite qualitative checking method and reagent kit |
| CN101398387A (en) * | 2008-10-28 | 2009-04-01 | 西安交通大学 | Preparation of am detection test paper for milk and solution and am detection method |
| WO2009092498A2 (en) * | 2007-12-13 | 2009-07-30 | Universität Wien | Interference based indicator for analyzing the age and/or quality of a natural product |
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| CN87103531A (en) * | 1987-05-13 | 1988-11-23 | 陕西省标准局西安地区劳动服务公司 | The method for quickly detecting of fresh milk adulterant |
| CN1595119A (en) * | 2004-07-06 | 2005-03-16 | 陕西科技大学 | Method for rapid detection of ammonium salts in fresh milk |
| WO2009092498A2 (en) * | 2007-12-13 | 2009-07-30 | Universität Wien | Interference based indicator for analyzing the age and/or quality of a natural product |
| CN101290323A (en) * | 2008-05-20 | 2008-10-22 | 内蒙古蒙牛乳业（集团）股份有限公司 | Cow milk and milk powder nitrite qualitative checking method and reagent kit |
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| 《中国卫生检验杂志》 20080731 刘汉方等 双硫腙四氯化碳法直接测定尿锌 1302-1303 1-4 第18卷, 第7期 2 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478570A (en) * | 2010-11-25 | 2012-05-30 | 内蒙古蒙牛乳业（集团）股份有限公司 | Qualitative detection method for detecting catalase in raw milk |
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