CN101805022B - Method for preparing barium tungstate nanometer double-line arrays by using DNA as templates - Google Patents
Method for preparing barium tungstate nanometer double-line arrays by using DNA as templates Download PDFInfo
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- CN101805022B CN101805022B CN2010101611729A CN201010161172A CN101805022B CN 101805022 B CN101805022 B CN 101805022B CN 2010101611729 A CN2010101611729 A CN 2010101611729A CN 201010161172 A CN201010161172 A CN 201010161172A CN 101805022 B CN101805022 B CN 101805022B
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Abstract
The invention discloses a method for preparing barium tungstate nanometer double-line arrays by using colon bacillus genome DNA as templates, which belongs to the technical field of nanometer materials. The method comprises the following steps: adding a barium nitrate solution into a colon bacillus genome DNA solution; uniformly mixing the solution; carrying out oscillation hatching for 48 to 72 h under the conditions of the temperature between 4 and 6 DEG C and the oscillation speed between 80 and 90 r/min; and then, adding the sodium tungstate solution for oscillation hatching for 48 to 72 h under the conditions of the temperature between 4 and 6 DEG C and the oscillation speed between 80 and 90 r/min; . When the mixed solution is heated for 6 to 8 h under the condition of the temperature between 80 and 85 DEG C, the barium tungstate nanometer double-line arrays using the colon bacillus genome DNA as templates can be obtained. The invention avoids the complicated preparation process of the conventional method, can be completed under the mild conditions of low temperature, normal pressure and the like, the technology is simple, the cost is low, and the reaction can be easily controlled, so the goal of using the colon bacillus genome DNA as templates for synthesizing the barium tungstate nanometer double-line arrays to construct nanometer devices can be realized.
Description
Technical field
The present invention relates to a kind of technical field of nano material, particularly relating to a kind of is the method that template prepares barium tungstate nanometer double-line arrays based on bacillus coli gene group DNA.
Background technology
BaWO with scheelite-type structure (tetragonal spheroidal)
4Crystal is a kind of very promising raman laser crystal, has interesting photoluminescence, thermoluminescence and stimulated Raman scattering characteristics such as (SRS), has important use and be worth in fields such as photoelectricity, medical science and spectroscopy.1999, people such as Muscovite Basiev found BaWO after having measured tungstate and the crystal-like Raman spectrum of other kind
4The crystalline Raman spectrum has bigger raman scattering cross section and Raman scattering intensity, so BaWO
4Crystal all has bigger Raman gain under nanosecond pulse or picosecond pulse laser effect, point out BaWO
4Crystal is a kind of very promising Raman crystal.BaWO
4As a kind of good Raman crystal, have the wave band that sees through of broad, the integrated intensity of higher Raman line and peak strength, this crystal is deliquescence not, and thermomechanical property is good.BaWO
4These characteristics of crystalline make it have stronger competitive edge in the application of Ramar laser, become in recent years the focus of research both at home and abroad.
Because it is the performance of nano material is not only relevant with the size of particle, also closely related with its pattern.Therefore, explore new preparation method, synthetic BaWO with different-shape
4Nano material has important significance for theories and potential using value.At present, preparation BaWO
4The method of nano material mainly contains solid phase method and liquid phase method: solid phase method comprises ball milled, and solid reaction process etc., liquid phase method comprise polymkeric substance companion method and reverse micelle microemulsion method etc.Qian research group (J.Cryst.Growth.2002,235,283-286) use hydrothermal synthesis method at different tensio-active agent (C
17H
33COOK, C
19H
39COOK and C
25H
51COOK) exist and to have obtained olive-shaped, sheet and whisker shape BaWO down respectively
4Nanoparticle.The shortcoming of this method is to need higher temperature and complex apparatus, and the reaction process complexity.Qi etc. (J.Am.Chem.Soc.2003,125,3450-3451) made featheriness BaWO with undeeanoic acid, decyl amine and polyethylene glycol-methacrylic acid segmented copolymer mixed system
4Etc. nanostructure.The shortcoming of this method is the reaction process complexity, the cost height, and this has hindered BaWO to a certain extent
4Application.
Summary of the invention
At existing preparation nanometer BaWO
4The technology above shortcomings, the invention provides a kind of method of utilizing bacillus coli gene group DNA to prepare barium tungstate nanometer double-line arrays for template, this method is utilized the critical nature of DNA sex change, and promptly the heat treated double-stranded DNA makes the DNA sex change become strand and prepares barium tungstate nanometer double-line arrays.
The technical scheme that the present invention is adopted for its technical problem of solution is: directly the biological nano structure bacillus coli gene group DNA that exists with nature is that template prepares barium tungstate nanometer double-line arrays, respectively with Ba (NO
3)
2And Na
2WO
4Solution joins in the bacillus coli gene group dna solution, vibration is hatched the regular hour through shaking table, then whole system is positioned under the certain temperature and heats the regular hour, finally can prepare with bacillus coli gene group DNA is the barium tungstate nanometer double-line arrays of template.
This preparation technology mainly comprises the steps:
(1) gets the Ba (NO of 5～10mM
3)
2 Solution 100～200 μ L join in the bacillus coli gene group dna solution, mix, and then at 4～6 ℃, 48～72h is hatched in 80～90 rev/mins of shaking table vibrations.
(2) in above-mentioned solution, add the Na of 5～10mM
2WO
4Solution 100～200 μ L, behind the mixing, in 4～6 ℃, 48～72h is hatched in 80～90 rev/mins of shaking table vibrations.
(3) then above-mentioned mixing solutions is positioned over 80～85 ℃ thermostat metal and heats 6～8h in bathing, can obtain with bacillus coli gene group DNA is the barium tungstate nanometer double-line arrays of template.
With bacillus coli gene group DNA is that method used bacillus coli gene group dna solution in above-mentioned steps (1) that template prepares barium tungstate nanometer double-line arrays is 100～200 μ L, its concentration is 1.03～2.10 μ g/ μ L, optical density(OD) (OD) value is 1.78～1.81, with preceding mixing repeatedly, DNA is scattered in the deionized water fully.
The invention has the beneficial effects as follows: the notable feature of this method is that the biological nano structure bacillus coli gene group DNA that directly exists with nature is a template, and utilize the critical nature of DNA sex change, it is the heat treated double-stranded DNA, make the DNA sex change become strand, prepare barium tungstate nanometer double-line arrays, technology is simple, and cost is low, reaction can be carried out under low-temperature atmosphere-pressure, has avoided the complicated technology of conventional synthetic method.Synthetic barium wolframate pattern is a nanometer double-line arrays, pattern rule, size evenly, for the preparation of novel nano-materials such as barium tungstate nanometer double-line arrays provides a practicable thinking of development.Simultaneously, the shown good excited Raman characteristic that goes out of barium tungstate nanometer double-line arrays is also laid a good foundation for the applied research of carrying out the barium tungstate nanometer material from now on.
Description of drawings
Fig. 1 is the transmission electron microscope photo of the barium tungstate nanometer double-line arrays of embodiment 1 preparation;
Fig. 2 is the transmission electron microscope photo of the barium tungstate nanometer double-line arrays of embodiment 2 preparations;
Fig. 3 is the transmission electron microscope photo of the barium tungstate nanometer double-line arrays of embodiment 3 preparations;
Fig. 4 is the EDS collection of illustrative plates of the barium tungstate nanometer double-line arrays of embodiment 1 preparation;
Fig. 5 is the XRD figure spectrum of the barium tungstate nanometer double-line arrays of embodiment 1 preparation;
Fig. 6 is the excited Raman collection of illustrative plates of the barium tungstate nanometer double-line arrays of embodiment 1 preparation.
Embodiment
With 100 μ L concentration is 1.03 μ g/ μ L, and optical density(OD) (OD) value is 1.78 the abundant mixing of bacillus coli gene group dna solution, and DNA is scattered in the deionized water fully.Ba (the NO that in above-mentioned bacillus coli gene group dna solution, adds 5mM then
3)
2 Solution 100 μ L, fully behind the mixing, in 4 ℃, 48h is hatched in concussion on 80 rev/mins of shaking tables.The Na that in above-mentioned mixing solutions, adds 5mM
2WO
4Solution 100 μ L mix.In 4 ℃, 48h is hatched in concussion on 80 rev/mins of shaking tables.At last above-mentioned mixing solutions is positioned in 80 ℃ of thermostat metals baths and heats 8h.
With 100 μ L concentration is 2.10 μ g/ μ L, and optical density(OD) (OD) value is 1.81 the abundant mixing of bacillus coli gene group dna solution, and DNA is scattered in the deionized water fully.Ba (the NO that in above-mentioned bacillus coli gene group dna solution, adds 10mM then
3)
2 Solution 100 μ L, fully behind the mixing, in 5 ℃, 60h is hatched in concussion on 85 rev/mins of shaking tables.The Na that in above-mentioned mixing solutions, adds 10mM
2WO
4Solution 100 μ L mix.In 5 ℃, 60h is hatched in concussion on 85 rev/mins of shaking tables.At last above-mentioned mixing solutions is positioned in 80 ℃ of thermostat metals baths and heats 7h.
With 200 μ L concentration is 1.60 μ g/ μ L, and optical density(OD) (OD) value is 1.79 the abundant mixing of bacillus coli gene group dna solution, and DNA is scattered in the deionized water fully.Ba (the NO that in above-mentioned bacillus coli gene group dna solution, adds 5mM then
3)
2 Solution 200 μ L, fully behind the mixing, in 6 ℃, 72h is hatched in concussion on 90 rev/mins of shaking tables.The Na that in above-mentioned mixing solutions, adds 5mM
2WO
4Solution 200 μ L mix.In 6 ℃, 72h is hatched in concussion on 90 rev/mins of shaking tables.At last above-mentioned mixing solutions is positioned in 85 ℃ of thermostat metals baths and heats 6h.
Fig. 1 is the transmission electron microscope photo of the barium tungstate nanometer double-line arrays of embodiment 1 preparation, as can be seen from the figure, the barium wolframate of preparing is good nanometer double-line arrays pattern, because this sample does not pass through negative staining but directly detects under transmission electron microscope, so can prove that the barium tungstate nanometer particle combines with the DNA chain is good, and it is black to have demonstrated outer rim, the double-line arrays of central, clear.The wide about 12～19nm of the external diameter of the barium tungstate nanometer double-line arrays that makes, the about 6～10nm of internal diameter.Demonstrate rule of this preparation method's synthetic barium tungstate nanometer double-line arrays pattern, size is more even, can realize controllable growth.
Fig. 2 is the transmission electron microscope photo of the barium tungstate nanometer double-line arrays of embodiment 2 preparations, as can be seen from the figure, because the increase of barium wolframate concentration, be greater than the speed of heteronuclear growth with the speed of nucleus growth, so the deposition of barium wolframate particle on the DNA chain is not very even, but still can generate nanometer double-line arrays.The barium tungstate nanometer double-line arrays that therefore, can prepare the pattern rule by the control concentration of reactants.
Fig. 3 is the transmission electron microscope photo of the barium tungstate nanometer double-line arrays of embodiment 3 preparations, can obviously find out the barium tungstate nanometer double-line arrays pattern from figure.Because the corresponding rising of temperature, make the unfolded wider width of dna double chain, so the external diameter broad of the barium tungstate nanometer double-line arrays that makes, pattern is regular but the barium tungstate nanometer double-line arrays that makes remains unchanged.Therefore, can make the barium tungstate nanometer double-line arrays of certain width, make that utilizing bacillus coli gene group dna molecular to construct nano-device as the template of synthetic barium tungstate nanometer double-line arrays becomes possibility by the control Heating temperature.
Fig. 4 is the EDS collection of illustrative plates of the barium tungstate nanometer double-line arrays of embodiment 1 preparation.Can see that from collection of illustrative plates the barium tungstate nanometer double-line arrays that we prepare is elementary composition by W, Ba and O, C and Cu peak are that the P peak is from DNA owing to make due to the substrate of copper mesh among the figure, and the Na peak is from Na
2WO
4So, there is not other impurity component, hence one can see that, and sample is the very high barium wolframate of purity.
Fig. 5 is the XRD figure spectrum of the barium tungstate nanometer double-line arrays of embodiment 1 preparation.XRD figure spectrum and standard card contrast are learnt that the barium wolframate that we make is cubic scheelite-type structure, and the unit cell parameters that calculates is a=5.562
C=12.701
° peak of locating does not have impurity peaks to exist from DNA in 2 θ=21.3.Therefore, the barium tungstate crystal well-crystallized of this method preparation, purity is higher.
Fig. 6 is the excited Raman collection of illustrative plates of the barium tungstate nanometer double-line arrays of embodiment 1 preparation.From measured result, be that the barium tungstate nanometer double-line arrays that template prepares is a tetragonal with bacillus coli gene group DNA.925,831,795 and 333cm
-1The peak value at place depends on different vibrating modes, corresponds respectively to υ 1 (Ag), υ 3 (Bg), υ 3 (Eg) and υ 2 (Bg).At 924cm
-1Place's intensity is the highest, shows that the SRS gain factor of product is higher.At 1122cm
-1The peak value at place derives from DNA..Raman collection of illustrative plates further proof is that the barium tungstate nanometer double-line arrays that template prepares has good excited Raman performance with bacillus coli gene group DNA, is expected at light, has more wide practical value in the field of nanometer devices of electricity and catalytic activity.
Claims (1)
1. one kind is utilized bacillus coli gene group DNA for template prepares the method for barium tungstate nanometer double-line arrays, it is characterized in that: said method comprising the steps of:
(1) gets the Ba (NO of 5～10mM
3)
2Solution 100～200 μ L join in the bacillus coli gene group dna solution, mix, and then at 4～6 ℃, 48～72h is hatched in 80～90 rev/mins of shaking table vibrations;
(2) in above-mentioned solution, add the Na of 5～10mM
2WO
4Solution 100～200 μ L, behind the mixing, in 4～6 ℃, 48～72h is hatched in 80～90 rev/mins of shaking table vibrations;
(3) then above-mentioned mixing solutions is positioned over 80～85 ℃ thermostat metal and heats 6～8h in bathing, can obtain with bacillus coli gene group DNA is the barium tungstate nanometer double-line arrays of template;
Used bacillus coli gene group dna solution is 100～200 μ L in the step (1), and its concentration is 1.03～2.10 μ g/ μ L, and optical density(OD) (OD) value is 1.78～1.81, with preceding mixing repeatedly, DNA is scattered in the deionized water fully.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1923706A (en) * | 2006-09-14 | 2007-03-07 | 上海交通大学 | Preparation method of dendroid barium tungstate nano crystal |
| US20070113779A1 (en) * | 2005-11-02 | 2007-05-24 | The Research Foundation Of State University Of New York | Metal oxide and metal fluoride nanostructures and methods of making same |
| CN101372357A (en) * | 2008-09-27 | 2009-02-25 | 燕山大学 | Method for controlling micrometre zinc sulphide morphology by Escherichia coli biological template |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070113779A1 (en) * | 2005-11-02 | 2007-05-24 | The Research Foundation Of State University Of New York | Metal oxide and metal fluoride nanostructures and methods of making same |
| CN1923706A (en) * | 2006-09-14 | 2007-03-07 | 上海交通大学 | Preparation method of dendroid barium tungstate nano crystal |
| CN101372357A (en) * | 2008-09-27 | 2009-02-25 | 燕山大学 | Method for controlling micrometre zinc sulphide morphology by Escherichia coli biological template |
Non-Patent Citations (2)
| Title |
|---|
| Hongtao Shi et al..Polymer-Directed Synthesis of Penniform BaWO4 Nanostructures in Reverse Micelles.《J. AM. CHEM. SOC.》.2003,3450-3451. * |
| 周萍 等.不同形状的钨酸钡纳米粒子的合成.《应用化学》.2006,第23卷(第4期),370-373. * |
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