CN101799438A - Method for detecting clonorchis sinensis eggs in gallstone - Google Patents
Method for detecting clonorchis sinensis eggs in gallstone Download PDFInfo
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- CN101799438A CN101799438A CN201010123552A CN201010123552A CN101799438A CN 101799438 A CN101799438 A CN 101799438A CN 201010123552 A CN201010123552 A CN 201010123552A CN 201010123552 A CN201010123552 A CN 201010123552A CN 101799438 A CN101799438 A CN 101799438A
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- clonorchis sinensis
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Abstract
The invention relates to a method for detecting clonorchis sinensis eggs, in particular to a method for detecting clonorchis sinensis eggs in gallstone, comprising the following steps: 1) firstly grinding stones and filtering the ground stones to prepare platelets; 2) observing whether the platelets contain clonorchis sinensis eggs through an optical microscope; 3) observing the platelets or the sectioned stones through a scanning electron microscope if the clonorchis sinensis eggs are not observed; and 4) detecting the clonorchis sinensis eggs by polymerase chain reaction if the clonorchis sinensis eggs are not observed. By detecting the clonorchis sinensis eggs in gallstone, the invention obviously increases the detection rate of infection caused by clonorchis sinensis, provides a new method for clinically preventing and treating infection caused by clonorchis sinensis and exploring the correlation between the gallstone and infection caused by clonorchis sinensis so as to reduce the incidence of the gallstone, improves the precision of diagnosis and achieves the aims of early diagnosis, early treatment and early prevention.
Description
Technical field
The present invention relates to a kind of method that detects clonorchis sinensis eggs, be specifically related to detect in a kind of cholelith the method for clonorchis sinensis eggs.
Background technology
Gall stone is one of common disease, frequently-occurring disease of general surgery in the present worldwide, and the incidence of disease is about 10% in the crowd.Clonorchis sinensis has popular very widely in China, influence the crowd in China and have 8.7 hundred million at least, and infection population is estimated to reach 1,500 ten thousand, is the most important food source property parasite of popular China.Microscopy finds that clonorchis sinensis eggs is to make a definite diagnosis the main method that clonorchis sinensis infects, at present main adopt ight soil and scope to obtain the intestinal juice that duodenum contains bile after, the method of carrying out the ordinary optical microscope microscopy of laminating detects clonorchis sinensis eggs, the result shows that the direct slice method recall rate of ight soil is minimum, bile precipitation microscopy recall rate is about 42.6%, recall rate is low, cause easily and fail to pinpoint a disease in diagnosis, cause the high rate of the high relapse rate and the cholecystolithiasis of postoperative cholecystolithiasis, incured loss through delay the opportunity that clinical prevention treatment clonorchis sinensis infects.
Summary of the invention
Result of study shows that clonorchis sinensis infects and cholecystolithiasis has closely related property at present, at ordinary optical microscopy method, cause the shortcoming of clonorchis sinensis eggs omission easily, the present invention proposes to detect in a kind of cholelith the new method of clonorchis sinensis eggs, improved the recall rate that clonorchis sinensis infects, infect and explore the correlativity of cholecystolithiasis and clonorchis sinensis infection for clinical prevention treatment clonorchis sinensis, thereby for the incidence that reduces cholecystolithiasis provides new method, reach diagnosis early, early treatment, the early purpose of prevention.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions:
Detect the method for clonorchis sinensis eggs in a kind of cholelith,
1) at first calculus is ground, laminate after the filtration;
2) whether contain clonorchis sinensis eggs by the above-mentioned thin slice of observation by light microscope;
3), above-mentioned thin slice or the calculus cut open are observed by scanning electron microscope if do not observed clonorchis sinensis eggs;
4) if do not observe clonorchis sinensis eggs yet, detect by polyase chain reaction detecting method.
Further, described calculus adds physiological saline and grinds, and is coated with flakiness by adding physiological saline after the filtered through gauze.
Further, described thin slice is to be at least 100 times low power optical microscope to scan whole thin slice by enlargement ratio earlier, and then is that Powerful Light Microscope more than 400 times is observed its microtexture with enlargement ratio.
Further, described polymerase chain reaction detection is to utilize the special gene primer of clonorchis sinensis that suspicious specimen is carried out DNA analysis.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention detects clonorchis sinensis eggs by cholelith, significantly improved the recall rate that clonorchis sinensis infects, infect and explore the correlativity of cholecystolithiasis and clonorchis sinensis infection for clinical prevention treatment clonorchis sinensis, thereby for the incidence that reduces cholecystolithiasis provides new method, increased the accuracy of diagnosis, reach diagnosis early, early treatment, the early purpose of prevention.
Embodiment
Detect the method for clonorchis sinensis eggs in the cholelith of the present invention, at first get 1～3 of cholecystolithiasis, diameter 0.2～2.2cm adds an amount of physiological saline, grinds calculus with mortar, adds an amount of physiological saline again, filters more than or equal to 260 order nylon yarn.Add that a small amount of physiological saline is coated with flakiness and mark is treated microscopy well.2～3 of every part of calculus thin slices.Earlier scan whole thin slice with optical microscope then, and then,, just no longer carry out following step if observe clonorchis sinensis eggs with 400 times of Powerful Light Microscope or above observation microtexture at low power lens (at least 100 times).
If do not observed clonorchis sinensis eggs, observe by scanning electron microscope again, the scanning electron microscope microscopy is to position suspicious in the above-mentioned thin slice, at least amplify 7 times～1,000,000 times or more than observe, carry out microscopy after also can directly cutting open simultaneously to the part calculus, if observe clonorchis sinensis eggs, just no longer carry out following step.
If do not observed clonorchis sinensis eggs yet, detect by polymerase chain reaction (PCR) detection method at last.It is that calculus is filtered the ordinary optical microscope microscopy, the scanning electron microscope microscopy is difficult to observe clonorchis sinensis eggs utilizing above-mentioned grinding that polymerase chain reaction detects, to utilize the special gene primer of clonorchis sinensis that suspicious specimen is carried out DNA analysis, with accurate judgement patient's pathogenic infection.
The present invention is not limited to above-mentioned embodiment, if various changes of the present invention or distortion are not broken away from the spirit and scope of the present invention, if these changes and distortion belong within claim of the present invention and the equivalent technologies scope, then the present invention also is intended to comprise these changes and distortion.
Claims (4)
1. detect the method for clonorchis sinensis eggs in the cholelith, it is characterized in that:
1) at first calculus is ground, laminate after the filtration;
2) whether contain clonorchis sinensis eggs by the above-mentioned thin slice of observation by light microscope;
3), above-mentioned thin slice or the calculus cut open are observed by scanning electron microscope if do not observed clonorchis sinensis eggs;
4) if do not observe clonorchis sinensis eggs yet, detect by polyase chain reaction detecting method.
2. detect the method for clonorchis sinensis eggs in the cholelith according to claim 1, it is characterized in that: described calculus adds physiological saline and grinds, and is coated with flakiness by adding physiological saline after the filtered through gauze.
3. detect the method for clonorchis sinensis eggs in the cholelith according to claim 1, it is characterized in that: described thin slice is to be at least 100 times low power optical microscope to scan whole thin slice by enlargement ratio earlier, and then is that Powerful Light Microscope more than 400 times is observed its microtexture with enlargement ratio.
4. detect the method for clonorchis sinensis eggs in the cholelith according to claim 1, it is characterized in that: it is to utilize the special gene primer of clonorchis sinensis that suspicious specimen is carried out DNA analysis that described polymerase chain reaction detects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010123552A CN101799438A (en) | 2010-03-10 | 2010-03-10 | Method for detecting clonorchis sinensis eggs in gallstone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010123552A CN101799438A (en) | 2010-03-10 | 2010-03-10 | Method for detecting clonorchis sinensis eggs in gallstone |
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| CN101799438A true CN101799438A (en) | 2010-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201010123552A Pending CN101799438A (en) | 2010-03-10 | 2010-03-10 | Method for detecting clonorchis sinensis eggs in gallstone |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102998319A (en) * | 2011-09-15 | 2013-03-27 | 广州市番禺区胆囊病研究所 | Method for studying microstructure of clonorchis sinensis egg in gallstone |
| CN111240004A (en) * | 2020-03-11 | 2020-06-05 | 北京埃鲁克技术检测有限责任公司 | System and method for automatically identifying two insects by microscope |
-
2010
- 2010-03-10 CN CN201010123552A patent/CN101799438A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102998319A (en) * | 2011-09-15 | 2013-03-27 | 广州市番禺区胆囊病研究所 | Method for studying microstructure of clonorchis sinensis egg in gallstone |
| CN102998319B (en) * | 2011-09-15 | 2015-04-01 | 广州市番禺区胆囊病研究所 | Method for studying microstructure of clonorchis sinensis egg in gallstone |
| CN111240004A (en) * | 2020-03-11 | 2020-06-05 | 北京埃鲁克技术检测有限责任公司 | System and method for automatically identifying two insects by microscope |
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Application publication date: 20100811 |