CN101799329B - Method for detecting color of Chinese herbal tablet - Google Patents

Abstract

The invention discloses a method for detecting the color of the Chinese herbal tablet, comprising the followings steps: converting the information on the color of the contrasted Chinese herbal tablet and the Chinese herbal tablet to be detected into the CIELab value, and calculating the difference between the color of the contrasted Chinese herbal tablet and the color of the Chinese herbal tablet to be detected according to the CIEDE2000 color difference calculation formula. The method can be used for quantitatively describing the color of the Chinese herbal tablet and the difference between the color of the contrasted Chinese herbal tablet and the color of the Chinese herbal tablet to be detected so as to change the status quo of the traditional method for judging the color of the Chinese herbal tablet fully by the subjective feeling and experience and better ensure the quality of the Chinese herbal tablet. The CIELab value is obtained in such a way of putting the contrasted Chinese herbal tablet and the Chinese herbal tablet to be detected on the white background, taking a picture for the contrasted Chinese herbal tablet by using a CCD camera when a certain condition being met, collecting the picture for the contrasted Chinese herbal tablet in a computer and converting the information on the color of the contrasted Chinese herbal tablet in the picture into the CIELab value.

Description

A kind of method that detects prepared slices of Chinese crude drugs appearance luster

Technical field

The present invention relates to the colorimetry field, relate in particular to a kind of detection method of prepared slices of Chinese crude drugs appearance luster.

Background technology

The color on measure solid surface; In some industry prerequisite and means as the product quality Detection & Controling; Like industries such as printing, weaving, printing and dyeing, the size of value of chromatism directly affects the evaluation of product quality grade, and the client is to the approval degree of product.In the control of the processing quality of the prepared slices of Chinese crude drugs and selling, equally with the medicine materical crude slice appearance luster as one of leading indicator of judging the quality quality.In Chinese medicine preparation, the consumption of the size of the duration and degree of heating, the length of concocted time, auxiliary material etc. all can be directly reflected on the color and luster of concocting the back medicine materical crude slice, and the chemical constitution and the biological action of medicine materical crude slice concocted in final influence.Prepared slices of Chinese crude drugs appearance luster quality assessment at present also rests on the subjective judgement, entirely with the macroscopic experience of operating personnel.Though this can reflect quality, the craft feature of medicine materical crude slice relatively, because of it has sizable subjectivity, can't realize quantizing, be difficult to control prepared slice quality consistance, make prepared slice quality unstable.

Certain observe and lighting condition under the color of any object, all available 3 digital parameters are described, Here it is color coordinates that people say.All colours can be organized into a three dimensions, also claims color space.Commonly used now is that ClELAB and two uniform colour spaces of CIELUV that International Commission on Illumination (CIE) is recommended are represented light source colour or object color and aberration thereof, and wherein CIELAB is more commonly used.The CIELAB color space is to utilize L, a, three different coordinate axis of b, position and the code name of indication color in geometric coordinate figure.It is based on a kind of color can not be simultaneously green not only red, be indigo plant but also be yellow this theory and setting up.When a kind of color was represented with CIELab, the L axle was represented lightness, deceived in the bottom, and in vain on the top :+a representes redness, and-a representes green; + b representes yellow, and-b representes blueness.The color change of any color can be used a, and b numerical value representes that the level of any color changes and can represent with the L value, uses L, a, and three numerical value of b just can be described any color of occurring in nature.Aberration representes that with regard to being meant with the mode of numerical value two kinds of colors are to the difference on the human eye perceives.If two colored samples all press L, a, b demarcates color, then between the two total color difference and the general available formula calculating of each individual event aberration:

Total color difference Δ E _Ab=[(Δ L) ²+ (Δ is a) ²+ (Δ b) ²] ^1/2

Luminosity equation Δ L=L ₁-L ₂(lightness difference)

Colour difference Δ a=a ₁-a ₂. (red/green difference)

Δ b=b ₁-b ₂. (Huang/blue difference)

Wherein 1 is sample, and 2 is standard sample (" the CIELAB color space and application thereof ", Zhao Xiuping, printing quality and standardization, 2005, (5): 11-13).

But above-mentioned formula can not correctly be expressed the visual experience of human eye based on an inhomogeneous color space; Therefore many

Subscript s and b indicate to calculate contrast color and the sample look in a pair of color of aberration respectively, down with; Δ h wherein _Ab'=h _{Ab, b}'-h _{Ab, s}'.

In the 4th step, calculate S _L, S _cAnd S _H, and R _TAnd R _C

S _L, S _cAnd S _HBe called weighting function, purpose is the homogeneity of proofreading and correct the CIE color space.It is defined as follows:

$S_L=1+\frac{0.015\×{(\stackrel{\‾}{L^{\′}}-50)}^2}{\sqrt{20+{(\stackrel{\‾}{L^{\′}}-50)}^2}}$ $S_C=1+0.045\×\stackrel{\‾}{C_{\mathrm{ab}}^{\′}}$ $S_H=1+0.015\×\stackrel{\‾}{C_{\mathrm{ab}}^{\′}}\×T$

Wherein,

$T=1-0.17\×\cos (\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}-{30}^0)+0.24\×\cos \left(2\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}\right)+0.32\×\cos (3\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}+6^0)-0.2\×\cos (4\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}-{63}^0)$

The L ', the h that divide two colors calculating _Ab' arithmetic mean.

R _T＝-sin(2Δθ)×R _C

Wherein,

it be rotation angle by tone decision;

$R_C=2\sqrt{\frac{{\stackrel{\‾}{C_{\mathrm{Ab}}}}^7}{{\stackrel{\‾}{C_{\mathrm{Ab}}}}^7+{25}^7}},$ It is the rotation amplitude that changes according to chroma.

The 5th step, K _L, K _CAnd K _HSelection.

Parameter factors K _L, K _CAnd K _HBe the correction coefficient relevant with service condition. they are the factors that affect the aberration sensation.K under the given standard observation condition of CIE _L=K _C=K _H=1.CIE specified standard condition is: D ₆₅Light source, illumination 1000lx, greater than 4 ° of visual fields, color even.

In the 6th step, calculate aberration with formula (1).

In the reality, colour photometer just can be accomplished above-mentioned measurements and calculations process, thus can measure intuitively and demarcate the solid surface color and and check sample between aberration, colour photometer is widely used in fields such as weaving, printings.But when colour photometer is measured the solid surface color, require the measured object surface necessary smooth, smooth, and, between colour photometer and the measured object slit can not be arranged in order to guarantee single light source incident.As everyone knows, the prepared slices of Chinese crude drugs come in every shape, the most surfaces out-of-flatness, and can not reach to have this requirement of slit between colour photometer and the measured object.Therefore, can not measure the color and luster of prepared slices of Chinese crude drugs outward appearance, be necessary to develop the new method that detects prepared slices of Chinese crude drugs appearance luster with the colour photometer of routine.

Summary of the invention

On the basis of year research, CIE had announced up-to-date aberration computing formula: CIEDE2000 (1) in 1999, and this colour difference formula is present best in theory colour difference formula.

${\mathrm{\ΔE}}_{\mathrm{ab}}=\sqrt{{\left(\frac{\mathrm{\Δ}{L}^{\′}}{K_L{S}_L}\right)}^2+{\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)}^2+{\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}^2+R_T\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}---\left(1\right)$

Zheng Yuanlin etc. have introduced the computation process of CIEDE2000 and the meaning of each parameter (" up-to-date colour difference formula: CIEDE2000 " in detail on " printing quality and standardization " the 7th phase in 2004; Zheng Yuanlin; Liu Shiwei, " printing quality and standardization ", 2004 (4): 34-37).

At first, calculate L, a, b, C at the CIELAB color space _AbEquivalent.

$L=116\×f\left(\frac{Y}{Y_n}\right)-16$

$a=500\×[f\left(\frac{X}{X_n}\right)-f\left(\frac{Y}{Y_n}\right)]$

$b=200\×[f\left(\frac{Y}{Y_n}\right)-f\left(\frac{Z}{Z_n}\right)]$

$C_{\mathrm{ab}}=\sqrt{a^2+b^2}$

Wherein, $f\left(I\right)=I^{\frac{1}{3}},I>0.008856$

$f\left(I\right)=7.787\×I+\frac{16}{116},I\≤0.008856$

Calculate a ' and C ' then, h '.

L′＝L a′＝(1+G)×a b′＝b $C_{\mathrm{ab}}^{\′}=\sqrt{a^{\′2}+b^{\′2}}$ $h^{\′}={\tan }^{-1}\left(\frac{b^{\′}}{a^{\′}}\right)$

Wherein, $G=0.5\×(1-\sqrt{\frac{{\stackrel{\‾}{C_{\mathrm{Ab}}}}^7}{{\stackrel{\‾}{C_{\mathrm{Ab}}}}^7+{25}^7}}),$ The adjustment factor of a axle of its expression CIELAB color space is the function of chroma.Here,

Be the C of two colors will calculating _AbArithmetic mean.

In the 3rd step, calculate Δ L ', Δ C ' _Ab, Δ H ' _Ab

Δ L ', Δ C ' _Ab, Δ H ' _AbRepresent luminosity equation, chroma difference and hue difference respectively, computing formula is following:

ΔL′＝L _b′-L _s′ ΔC _ab′＝C _ab，b′-C _ab，s′

$\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}=2\×\sqrt{C_{\mathrm{ab},b}^{\′}\×C_{\mathrm{ab},s}^{\′}}\×\sin \left(\frac{\mathrm{\Δ}{h}_{\mathrm{ab}}^{\′}}{2}\right)$

The object of the present invention is to provide a kind of method that detects prepared slices of Chinese crude drugs appearance luster; On the basis of quantificational description prepared slices of Chinese crude drugs appearance luster; Can judge the aberration between the tested prepared slices of Chinese crude drugs and the contrast medicine materical crude slice objectively; Change tradition and rely on subjective feeling and experience to judge the present situation of prepared slices of Chinese crude drugs color and luster fully, thereby can guarantee the quality of the prepared slices of Chinese crude drugs better.

In order to solve the problems of the technologies described above, the technical scheme that the present invention adopts is:

A kind of method that detects prepared slices of Chinese crude drugs appearance luster mainly may further comprise the steps:

1) quantification of contrast prepared slices of Chinese crude drugs appearance luster

To contrast the prepared slices of Chinese crude drugs and place on the white background, CIE specified standard light source D ₆₅Directly over the said contrast prepared slices of Chinese crude drugs; The intensity of illumination of said light source is 1000-2000lx, takes the picture of the said contrast prepared slices of Chinese crude drugs by the CCD camera, gathers computing machine; The colouring information of the prepared slices of Chinese crude drugs of contrast described in the picture is converted into the CIELab value; Wherein the L value is described the brightness of color, and a value is described the chromatic value of color from the green to the redness, and b describes the chromatic value of color from the blueness to the yellow;

2) quantification of tested prepared slices of Chinese crude drugs appearance luster

Under the condition identical with step 1, take the picture of the said tested prepared slices of Chinese crude drugs of picked at random by the CCD camera, gather computing machine, the colouring information of the tested prepared slices of Chinese crude drugs described in the picture is converted into the CIELab value;

3) calculating of aberration

The said contrast prepared slices of Chinese crude drugs that step 1 and 2 is obtained and the CIELab value substitution formula (1) of the said tested prepared slices of Chinese crude drugs,

${\mathrm{\ΔE}}_{\mathrm{ab}}=\sqrt{{\left(\frac{\mathrm{\Δ}{L}^{\′}}{K_L{S}_L}\right)}^2+{\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)}^2+{\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}^2+R_T\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}---\left(1\right)$

CIELab value with said contrast prepared slices of Chinese crude drugs appearance luster is contrast, calculates said tested prepared slices of Chinese crude drugs appearance luster and the aberration that contrasts prepared slices of Chinese crude drugs appearance luster;

4) evaluation of prepared slices of Chinese crude drugs appearance luster

Repeating step 2 and step 3 obtain the aberration of many batches of tested prepared slices of Chinese crude drugs appearance luster, confirm the aberration acceptable ranges.

More representative in order to guarantee to measure the result, the present invention preferably adopts the mean value of CIELab value of the above prepared slices of Chinese crude drugs of a slice as the CIELab value of the contrast prepared slices of Chinese crude drugs or the tested prepared slices of Chinese crude drugs, and concrete steps comprise:

1) quantification of contrast prepared slices of Chinese crude drugs appearance luster

A slice or above mutual nonoverlapping contrast prepared slices of Chinese crude drugs are placed on the white background CIE specified standard light source D ₆₅Directly over the said contrast prepared slices of Chinese crude drugs, the intensity of illumination of said light source is 1000-2000lx, is taken the picture of the said contrast prepared slices of Chinese crude drugs by the CCD camera; Gather computing machine; The colouring information of every or each said contrast prepared slices of Chinese crude drugs in the picture is separately converted to the CIELab value, and with the CIELab value of mean value as the said contrast prepared slices of Chinese crude drugs, wherein the L value is described the brightness of color; The a value is described the chromatic value of color from the green to the redness, and b describes the chromatic value of color from the blueness to the yellow;

2) quantification of tested prepared slices of Chinese crude drugs appearance luster

Under the condition identical with step 1; Picture by mutual nonoverlapping said tested prepared slices of Chinese crude drugs more than a slice of CCD camera shooting picked at random; Gather computing machine; The colouring information of every or each said tested prepared slices of Chinese crude drugs in the picture is separately converted to the CIELab value, with the CIELab value of mean value as the said tested prepared slices of Chinese crude drugs;

3) calculating of aberration

The said contrast prepared slices of Chinese crude drugs that step 1 and 2 is obtained and the CIELab value substitution formula (1) of the said tested prepared slices of Chinese crude drugs; CIELab value with said contrast prepared slices of Chinese crude drugs appearance luster is contrast, calculates said tested prepared slices of Chinese crude drugs appearance luster and the aberration that contrasts prepared slices of Chinese crude drugs appearance luster.

${\mathrm{\ΔE}}_{\mathrm{ab}}=\sqrt{{\left(\frac{\mathrm{\Δ}{L}^{\′}}{K_L{S}_L}\right)}^2+{\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)}^2+{\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}^2+R_T\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}---\left(1\right)$

4) evaluation of prepared slices of Chinese crude drugs appearance luster

Repeating step 2 and step 3 obtain the aberration of many batches of tested prepared slices of Chinese crude drugs appearance luster, confirm the aberration acceptable ranges.

In the inventive method, said light source D ₆₅Preferably be made up of incandescent lamp and fluorescent light, wherein the intensity of incandescent lamp accounts for 60-80%, remains the intensity into fluorescent light.

When the CCD camera was taken the picture of the said contrast prepared slices of Chinese crude drugs or the tested prepared slices of Chinese crude drugs, preferred pixel was greater than 5,000,000 CCD camera.

Said camera is apart from the preferred 10-30cm of the distance of medicine materical crude slice, and camera visual angle and light source angle are preferably less than 10 degree.

In the inventive method. preferably the colouring information of the said contrast prepared slices of Chinese crude drugs or the tested prepared slices of Chinese crude drugs is converted into the CIELab value with the software of Adobe Photoshop9.0 or above version; Concrete operations are: read in the color and luster information photo files of each batch medicine materical crude slice outward appearance respectively with Adobe Photoshop9.0, in " messagewindow " selection " Lab color " pattern of this software.Select medicine materical crude slice colour examining zone with the evil spirit rod, use " on average " order in the menu again, make the color and luster in the selected colour examining zone even.Just can directly read lustre index data L, a, b value in the selection area at " messagewindow ".

The contrast prepared slices of Chinese crude drugs according to the invention have country imperial, adopt the medicine materical crude slice of this national standard, identify the contrast medicine materical crude slice that is provided like the national biological product medicine; Do not have country imperial, like the prepared slices of Chinese crude drugs that Jiangxi camphor tree one band is concocted through " the camphor tree group method " of the uniqueness of successive dynasties formation, the then said contrast prepared slices of Chinese crude drugs are the medicine materical crude slice that experienced pharmaceutical worker differentiates, picks out.

The said contrast prepared slices of Chinese crude drugs that the present invention once takes or the maximum quantity of the tested prepared slices of Chinese crude drugs are by the camera lens visual field and the decision of medicine materical crude slice volume; Such as the rhizoma alismatis medicine materical crude slice; Because volume is big, generally once takes 5-6 piece medicine materical crude slice at most, Fructus Aurantii once can be taken 20-30 piece medicine materical crude slice.

The acceptability limit of tested prepared slices of Chinese crude drugs aberration according to the invention is generally less than 10, but can be because of the difference of prepared slices of Chinese crude drugs kind difference to some extent.

In the inventive method, camera is taken is actually the light that said prepared slices of Chinese crude drugs reflection source is launched, so light source, light intensity are the principal elements that influences the CIELab pH-value determination pH.Foundation below by test explanation the inventive method.

Test 1 camphor tree group method bran and fry rhizoma alismatis

Frying pan is heated to 260 ℃, adds the wheat bran of mixing thoroughly with honey, fry to wheat bran and smolder, add living rhizoma alismatis sheet 250g again, the 6min that stir-fries, the surface is the glassy yellow taking-up and cools, and sieve goes wheat bran promptly to get.

Help the method bran to fry the rhizoma alismatis rhizoma alismatis medicine materical crude slice from above-mentioned camphor tree; Pick out 100 conduct tests that meet the experience requirement most through experienced old pharmaceutical worker and use the contrast prepared slices of Chinese crude drugs; Remaining medicine materical crude slice is divided into three types of dark color, Neutral colour and light medicine materical crude slice according to the color and luster depth, as the tested prepared slices of Chinese crude drugs.

Test of the influence of 2 Different Light to the medicine materical crude slice color difference measurement

Test used light source and constitute, investigate under certain total light intensity the influence of the paired prepared slices of Chinese crude drugs color difference measurement of the intensity groups of said two kinds of light sources by incandescent lamp and fluorescent light.

6 of the contrast prepared slices of Chinese crude drugs of picked at random test 1 preparation; Each 6 of said dark color, Neutral colour and the light tested prepared slices of Chinese crude drugs; Under condition shown in the table 1, the method pictures taken of record is utilized Adobe Photoshop9.0 to handle picture and is obtained the CIELab value to specifications; And calculating the value of chromatism of each tested prepared slices of Chinese crude drugs sample with respect to the contrast prepared slices of Chinese crude drugs, the result sees table 2-4.

Table 1 Different Light is to the test condition of the influence of medicine materical crude slice color difference measurement

^a: refer to camera visual angle and light source angle; ^b: refer to the distance of camera apart from medicine materical crude slice.

The dark tested medicine materical crude slice color difference measurement result of table 2

The tested medicine materical crude slice color difference measurement of table 3 Neutral colour result

The light tested medicine materical crude slice color difference measurement result of table 4

The result shows, Different Light is bigger to the influence of medicine materical crude slice color difference measurement, and the intensity of incandescent lamp is between 60-80% the time, and it is stable to measure the result, so the intensity of preferred incandescent lamp accounts for total light intensity 60-80%, remains the light source composition into fluorescent light.In addition, no matter under which kind of light source condition, all much smaller than the aberration of dark and the light tested prepared slices of Chinese crude drugs, the result is consistent with visual inspection near the aberration of the tested prepared slices of Chinese crude drugs of Neutral colour of the contrast prepared slices of Chinese crude drugs.

Test of the influence of 3 different light intensity degree to the medicine materical crude slice color difference measurement

Test used light source and be made up of incandescent lamp and fluorescent light, wherein incandescent lamp intensity accounts for 80% of total light intensity, and fluorescent light intensity accounts for 20% of total light intensity, investigates the influence of different total light intensities to prepared slices of Chinese crude drugs color difference measurement.

6 of the contrast prepared slices of Chinese crude drugs of picked at random test 1 preparation; Each 6 of said dark color, Neutral colour and the light tested prepared slices of Chinese crude drugs; Under condition shown in the table 5, the method pictures taken of record is utilized Adobe Photoshop9.0 to handle picture and is obtained the CIELab value to specifications; And calculating the value of chromatism of each tested prepared slices of Chinese crude drugs sample with respect to the contrast prepared slices of Chinese crude drugs, the result sees table 6-8.

The result shows, light source is formed when certain, and the color difference measurement influence to three kinds of tested medicine materical crude slice when light intensity changes in the 1000-2000lx scope is little.

Test 4 camphor trees group method bran and fry confirming of rhizoma alismatis medicine materical crude slice colour difference acceptability limit

Test used light source and be made up of incandescent lamp and fluorescent light, wherein incandescent lamp intensity accounts for 80% of total light intensity, and fluorescent light intensity accounts for 20% of total light intensity, and total light intensity is 1000lx, investigates the colour difference variation range of the said prepared slices of Chinese crude drugs of different batches.

Concoct 10 batches of brans according to Test Example 1 described concocting method and fry the rhizoma alismatis medicine materical crude slice, 100 of each batch picked at random are as the tested prepared slices of Chinese crude drugs.

4～5 of the contrast prepared slices of Chinese crude drugs of picked at random test 1 preparation; And 4～5 of the tested prepared slices of Chinese crude drugs of above-mentioned each batch; The method pictures taken of putting down in writing to specifications; Utilize Adobe Photoshop9.0 to handle picture and obtain the CIELab value, and calculate the value of chromatism of each tested prepared slices of Chinese crude drugs sample with respect to the contrast prepared slices of Chinese crude drugs, the result sees table 9.

The tested medicine materical crude slice color difference measurement of table 9 different batches result

Table 5 different light intensity degree is to the test condition of the influence of medicine materical crude slice color difference measurement

The dark tested medicine materical crude slice color difference measurement result of table 6

The tested medicine materical crude slice color difference measurement of table 7 Neutral colour result

The light tested medicine materical crude slice color difference measurement result of table 8

The result shows that each batch camphor tree group method bran stir-fry medicine materical crude slice, combines with production is actual less than 6 with respect to the aberration that contrasts the prepared slices of Chinese crude drugs, and tentative camphor tree helps method bran stir-fry medicine materical crude slice aberration acceptability limit must not be greater than 8.0.

Above-mentioned test 2 and 3 result show; No matter under which kind of condition; Near the aberration of the tested prepared slices of Chinese crude drugs of Neutral colour of the contrast prepared slices of Chinese crude drugs all much smaller than the aberration of dark and the light tested prepared slices of Chinese crude drugs; The result is consistent with visual inspection; Explain that the inventive method has reached other purpose of quantification shade deviation, utilize the inventive method can change tradition and rely on subjective feeling and experience to judge the present situation of prepared slices of Chinese crude drugs color and luster fully, thereby objective foundation can be provided for the modern management of prepared slices of Chinese crude drugs quality.

Embodiment:

Below in conjunction with embodiment the present invention is further described, but the invention is not restricted to the following example.

Embodiment 1 camphor tree group method bran is fried the color measuring of stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice

Get living rhizoma alismatis medicine materical crude slice 250g, add salt solution, stir, seal vexed profit, take out.Pot is burnt to 260 ℃, be sprinkled into wheat bran, treat to add the good stir-baked RHIZOMA ALISMATIS with salt solution sheet of vexed profit after wheat bran is smoldered, the 6min that constantly stir-fries, it is golden yellow that the surface is, and taking-up cools, and sieve goes wheat bran promptly to get.Prepare 10 batches altogether.

Help the method bran to fry the stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice from above-mentioned camphor tree, pick out 100 conduct tests that meet the experience requirement most by experienced old pharmaceutical worker and use the contrast camphor tree to help method bran stir-fry stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice; 100 rhizoma alismatis medicine materical crude slice of difference picked at random help the method bran to fry the stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice as tested camphor tree from each batch again.

1) contrast camphor tree group method bran is fried the quantification of stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice appearance luster

Help the method bran to fry the stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice 5 mutual nonoverlapping contrast camphor trees and place on the white background D that incandescent lamp and fluorescent light constitute ₆₅Directly over said contrast rhizoma alismatis medicine materical crude slice, the intensity of illumination of said light source is 1000lx, and wherein incandescent lamp accounts for 80%, and fluorescent light accounts for 20%; Take said contrast camphor tree by the CCD camera of 5,000,000 pixels and help the method bran to fry the picture of stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice, camera is apart from medicine materical crude slice 10cm, and camera visual angle and light source angle 10 are spent.Computing machine is advanced in said picture collection; Open AdobePhotoshop9.0, read in said picture, in " messagewindow " selection " Lab color " pattern of this software.Select each sheet rhizoma alismatis as the colour examining zone with the evil spirit rod, use " on average " order in the menu again, make the color and luster in the selected colour examining zone even.Directly read lustre index data L, a, b value in the selection area at " messagewindow ", then obtain contrasting the Lab value of rhizoma alismatis medicine materical crude slice.

2) tested camphor tree helps the method bran to fry the quantification of stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice appearance luster

Under the condition identical with step 1; Take the picture that the mutual nonoverlapping said tested camphor trees of first 5 of picked at random group method bran is fried the stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice by the CCD camera; Gather computing machine, with the mean value of the Lab value of the tested rhizoma alismatis medicine materical crude slice of each sheet Lab value as the said tested prepared slices of Chinese crude drugs;

3) calculating of aberration

The said contrast rhizoma alismatis medicine materical crude slice that step 1 and 2 is obtained and the CIELab value substitution formula (1) of said tested rhizoma alismatis medicine materical crude slice,

${\mathrm{\ΔE}}_{\mathrm{ab}}=\sqrt{{\left(\frac{\mathrm{\Δ}{L}^{\′}}{K_L{S}_L}\right)}^2+{\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)}^2+{\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}^2+R_T\left(\frac{\mathrm{\Δ}{C}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)\left(\frac{\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}---\left(1\right)$

CIELab value with said contrast rhizoma alismatis medicine materical crude slice appearance luster is contrast, calculates said tested rhizoma alismatis medicine materical crude slice appearance luster and the aberration that contrasts rhizoma alismatis medicine materical crude slice appearance luster.

4) camphor tree group method bran is fried the evaluation of stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice appearance luster

Repeating step 2 and step 3, up to color difference measurement and the calculating of accomplishing all 10 batches tested rhizoma alismatis medicine materical crude slice appearance luster, the result sees table 10.

Table 10 different batches camphor tree group method bran is fried stir-baked RHIZOMA ALISMATIS with salt solution sheet color difference measurement result

According to experimental result, confirm that camphor tree group method bran fries stir-baked RHIZOMA ALISMATIS with salt solution medicine materical crude slice aberration acceptable ranges and must not be greater than 8.0.

Embodiment 2 pharmacopeia method salt are fried the color measuring of rhizoma alismatis medicine materical crude slice

Get living rhizoma alismatis medicine materical crude slice 250g, add salt solution, stir, seal vexed profit, take out.Pot is burnt to 300 ℃, add the good living rhizoma alismatis medicine materical crude slice of vexed profit, the 4min that constantly stir-fries, taking-up cools promptly and gets.Prepare 10 batches altogether.

Fry the rhizoma alismatis medicine materical crude slice from above-mentioned each batch pharmacopeia method salt, rule of thumb select 100 conduct tests that meet the pharmacopeia regulation most by old pharmaceutical worker and fry the rhizoma alismatis medicine materical crude slice with contrast pharmacopeia method salt; Again respectively from each batch 100 of picked at random fry the rhizoma alismatis medicine materical crude slice as tested pharmacopeia method salt.

According to the identical step of embodiment 1, under following condition, the tested pharmacopeia method salt of measuring and calculate all batches is fried the aberration of rhizoma alismatis medicine materical crude slice, and the result sees table 11.

The D that incandescent lamp and fluorescent light constitute ₆₅Directly over said contrast rhizoma alismatis medicine materical crude slice, the intensity of illumination of said light source is 1500lx, and wherein incandescent lamp accounts for 80%, and fluorescent light accounts for 20%; Take the picture that said pharmacopeia method salt is fried the rhizoma alismatis medicine materical crude slice by the CCD camera of 7,000,000 pixels, camera is apart from medicine materical crude slice 20cm, and camera visual angle and light source angle 5 are spent.

Table 11 different batches pharmacopeia method salt is fried rhizoma alismatis sheet color difference measurement result

According to experimental result, confirm that pharmacopeia method salt fries rhizoma alismatis medicine materical crude slice aberration acceptable ranges and must not be greater than 8.0.

The mensuration of embodiment 3 camphor trees group method honey stir-baked FRUCTUS AURANTII in bran color and luster

It is subsequent use to get 100 kilograms of living Fructus Aurantii medicine materical crude slice, gets 1.5 kilograms of honeybees and mixes with 10 kilograms of wheat bran, stirs.Pot is burnt to 200 ℃, be sprinkled into the above sweet bran that mixes, treat that the wheat bran back that smolders adds the livings Fructus Aurantii medicine materical crude slice of camphor tree group's method, constantly stir-frying was faint yellow to the surface in 2 minutes, and taking-up cools, and sieves and goes wheat bran promptly to get.Prepare 4 batches altogether.

From above-mentioned " camphor tree group method " sweet stir-baked FRUCTUS AURANTII in bran medicine materical crude slice, pick out 100 conduct tests that meet the experience requirement most through experienced old pharmaceutical worker and help the sweet stir-baked FRUCTUS AURANTII in bran medicine materical crude slice of method with the contrast camphor tree; Again respectively from each batch 100 Fructus Aurantii medicine materical crude slice of picked at random as tested camphor tree group method honey stir-baked FRUCTUS AURANTII in bran medicine materical crude slice.

According to the identical step of embodiment 1, under following condition, measure and calculate the aberration of the tested camphor tree group method honey stir-baked FRUCTUS AURANTII in bran medicine materical crude slice of all batches, the result sees table 12.

The D that incandescent lamp and fluorescent light constitute ₆₅Directly over said contrast rhizoma alismatis medicine materical crude slice, the intensity of illumination of said light source is 2000lx, and wherein incandescent lamp accounts for 70%, and fluorescent light accounts for 30%; Take the picture that camphor tree is helped method honey stir-baked FRUCTUS AURANTII in bran medicine materical crude slice by the CCD camera of 5,000,000 pixels, camera is apart from medicine materical crude slice 30cm, and camera visual angle and light source angle 8 are spent.

The method honey stir-baked FRUCTUS AURANTII in bran medicine materical crude slice color difference measurement result of table 12 different batches camphor tree group

According to experimental result, confirm that camphor tree group method honey stir-baked FRUCTUS AURANTII in bran aberration acceptable ranges must not be greater than 8.0.

Claims (4)

1. method that detects prepared slices of Chinese crude drugs appearance luster is characterized in that: mainly comprise:

1) quantification of contrast prepared slices of Chinese crude drugs appearance luster

To contrast the prepared slices of Chinese crude drugs and place on the white background, CIE specified standard light source D65 is perpendicular to directly over the said contrast prepared slices of Chinese crude drugs, and the intensity of illumination of said light source is 1000-2000lx; Take the picture of the said contrast prepared slices of Chinese crude drugs by the CCD camera; Gather computing machine, the colouring information that contrasts the prepared slices of Chinese crude drugs described in the picture is converted into the CIELab value, wherein the L value is described the brightness of color; The a value is described the chromatic value of color from the green to the redness, and b describes the chromatic value of color from the blueness to the yellow; Said camera is apart from medicine materical crude slice 10-30cm, and camera visual angle and light source angle are less than 10 degree;

2) quantification of tested prepared slices of Chinese crude drugs appearance luster

Under the condition identical with step 1, take the picture of the said tested prepared slices of Chinese crude drugs of picked at random by the CCD camera, gather computing machine, the colouring information of the tested prepared slices of Chinese crude drugs described in the picture is converted into the CIELab value;

3) calculating of aberration

The said contrast prepared slices of Chinese crude drugs that step 1 and 2 is obtained and the CIELab value substitution formula (1) of the said tested prepared slices of Chinese crude drugs,

${\mathrm{\ΔE}}_{\mathrm{ab}}=\sqrt{{\left(\frac{{\mathrm{\ΔL}}^{\′}}{K_L{S}_L}\right)}^2+{\left(\frac{{\mathrm{\ΔC}}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)}^2+{\left(\frac{{\mathrm{\ΔH}}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}^2+R_T\left(\frac{{\mathrm{\ΔC}}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)\left(\frac{{\mathrm{\ΔH}}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}---\left(1\right)$

CIELab value with said contrast prepared slices of Chinese crude drugs appearance luster is contrast, calculates said tested prepared slices of Chinese crude drugs appearance luster and the aberration that contrasts prepared slices of Chinese crude drugs appearance luster, and the implication of concrete computation process and each parameter is following:

At first, calculate L, a, b, C at the CIELAB color space _AbValue

$L=116\×f\left(\frac{Y}{Y_n}\right)-16$

$a=500\×[f\left(\frac{X}{X_n}\right)-f\left(\frac{Y}{Y_n}\right)]$

$b=200\×[f\left(\frac{Y}{Y_n}\right)-f\left(\frac{Z}{Z_n}\right)]$

$C_{\mathrm{ab}}=\sqrt{a^2+b^2}$

Wherein, $\begin{array}{cc}f\left(I\right)=I^{\frac{1}{3}}& I>0.008856\\ f\left(I\right)=7.787\×I+\frac{16}{116}& I\≤0.008856\end{array};$

Calculate a ' and C ' then, h '

L′＝L a′＝(1+G)×a b′＝b $C_{\mathrm{ab}}^{\′}=\sqrt{a^{\′2}+b^{\′2}}$ $h^{\′}={\tan }^{-1}\left(\frac{b^{\′}}{a^{\′}}\right)$

Wherein,

The adjustment factor of a axle of expression CIELAB color space is the function of chroma,

Be the C of two colors will calculating _AbArithmetic mean;

In the 3rd step, calculate Δ L ', Δ C ' _Ab, Δ H ' _Ab

ΔL′＝L′ _b-L′ _s ΔC′ _ab＝C′ _ab，b-C′ _ab，s

$\mathrm{\Δ}{H}_{\mathrm{ab}}^{\′}=2\×\sqrt{C_{\mathrm{ab},b}^{\′}\×C_{\mathrm{ab},s}^{\′}}\×\sin \left(\frac{{\mathrm{\Δh}}_{\mathrm{ab}}^{\′}}{2}\right)$

Subscript s and b indicate to calculate contrast color and the sample look in a pair of color of aberration respectively, down with,

Δ h ' wherein _Ab=h ' _{Ab, b}-h ' _{Ab, s}

In the 4th step, calculate S _L, S _cAnd S _H, and R _TAnd R _C

$S_L=1+\frac{0.015\×{(\stackrel{\‾}{L^{\′}}-50)}^2}{\sqrt{20+{(\stackrel{\‾}{L^{\′}}-50)}^2}}$ $S_C=1+0.045\×\stackrel{\‾}{C_{\mathrm{ab}}^{\′}}$ $S_H=1+0.015\×\stackrel{\‾}{C_{\mathrm{ab}}^{\′}}\×T$

Wherein,

$T=1-0.17\×\cos (\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}-{30}^0)+0.24\×\cos \left(2\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}\right)+0.32\×\cos (3\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}+6^0)-0.2\×\cos (4\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}-{63}^0)$

The L ', the h ' that divide two colors calculating _AbArithmetic mean

R _T＝-sin(2Δθ)×R _C

Wherein, It is the rotation angle by the tone decision for

$R_C=2\sqrt{\frac{{\stackrel{\‾}{C}}_{\mathrm{Ab}}^7}{{\stackrel{\‾}{C}}_{\mathrm{Ab}}^7+{25}^7}},$ It is the rotation amplitude that changes according to chroma;

The 5th step, K _L, K _CAnd K _HSelection

K _L＝K _C＝K _H＝l；

In the 6th step, calculate aberration with formula (1);

4) evaluation of prepared slices of Chinese crude drugs appearance luster

Repeating step 2 and step 3 obtain the aberration of many batches of tested prepared slices of Chinese crude drugs appearance luster, confirm the aberration acceptable ranges.

2. method that detects prepared slices of Chinese crude drugs appearance luster is characterized in that: mainly comprise:

1) quantification of contrast prepared slices of Chinese crude drugs appearance luster

Mutual nonoverlapping contrast prepared slices of Chinese crude drugs more than a slice are placed on the white background; CIE specified standard light source D65 is perpendicular to directly over the said contrast prepared slices of Chinese crude drugs, and the intensity of illumination of said light source is 1000-2000lx, is taken the picture of the said contrast prepared slices of Chinese crude drugs by the CCD camera; Gather computing machine; The colouring information of every or each said contrast prepared slices of Chinese crude drugs in the picture is separately converted to the CIELab value, and with the CIELab value of mean value as the said contrast prepared slices of Chinese crude drugs, wherein the L value is described the brightness of color; The a value is described the chromatic value of color from the green to the redness, and b describes the chromatic value of color from the blueness to the yellow; Said camera is apart from medicine materical crude slice 10-30cm, and camera visual angle and light source angle are less than 10 degree;

2) quantification of tested prepared slices of Chinese crude drugs appearance luster

Under the condition identical with step 1; Picture by mutual nonoverlapping said tested prepared slices of Chinese crude drugs more than a slice of CCD camera shooting picked at random; Gather computing machine; The colouring information of every or each said tested prepared slices of Chinese crude drugs in the picture is separately converted to the CIELab value, with the CIELab value of mean value as the said tested prepared slices of Chinese crude drugs;

3) calculating of aberration

The said contrast prepared slices of Chinese crude drugs that step 1 and 2 is obtained and the CIELab value substitution formula (1) of the said tested prepared slices of Chinese crude drugs,

${\mathrm{\ΔE}}_{\mathrm{ab}}=\sqrt{{\left(\frac{{\mathrm{\ΔL}}^{\′}}{K_L{S}_L}\right)}^2+{\left(\frac{{\mathrm{\ΔC}}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)}^2+{\left(\frac{{\mathrm{\ΔH}}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}^2+R_T\left(\frac{{\mathrm{\ΔC}}_{\mathrm{ab}}^{\′}}{k_c{S}_c}\right)\left(\frac{{\mathrm{\ΔH}}_{\mathrm{ab}}^{\′}}{k_H{S}_H}\right)}---\left(1\right)$

CIELab value with said contrast prepared slices of Chinese crude drugs appearance luster is contrast, calculates said tested prepared slices of Chinese crude drugs appearance luster and the aberration that contrasts prepared slices of Chinese crude drugs appearance luster, and the implication of concrete computation process and each parameter is following:

At first, calculate L, a, b, C at the CIELAB color space _AbValue

$L=116\×f\left(\frac{Y}{Y_n}\right)-16$

$a=500\×[f\left(\frac{X}{X_n}\right)-f\left(\frac{Y}{Y_n}\right)]$

$b=200\×[f\left(\frac{Y}{Y_n}\right)-f\left(\frac{Z}{Z_n}\right)]$

$C_{\mathrm{ab}}=\sqrt{a^2+b^2}$

Wherein, $\begin{array}{cc}f\left(I\right)=I^{\frac{1}{3}}& I>0.008856\\ f\left(I\right)=7.787\×I+\frac{16}{116}& I\≤0.008856\end{array};$

Calculate a ' and C ' then, h '

L′＝L a′＝(1+G)×a b′＝b $C_{\mathrm{ab}}^{\′}=\sqrt{a^{\′2}+b^{\′2}}$ $h^{\′}={\tan }^{-1}\left(\frac{b^{\′}}{a^{\′}}\right)$

Wherein,

The adjustment factor of a axle of expression CIELAB color space is the function of chroma,

Be the C of two colors will calculating _AbArithmetic mean;

In the 3rd step, calculate Δ L ', Δ C ' _Ab, Δ H ' _Ab

ΔL′＝L′ _b-L′ _s ΔC′ _ab＝C′ _ab，b-C′ _ab，s

${\mathrm{\ΔH}}_{\mathrm{ab}}^{\′}=2\×\sqrt{C_{\mathrm{ab},b}^{\′}\×C_{\mathrm{ab},s}^{\′}}\×\sin \left(\frac{{\mathrm{\Δh}}_{\mathrm{ab}}^{\′}}{2}\right)$

Subscript s and b indicate to calculate contrast color and the sample look in a pair of color of aberration respectively, down with,

Δ h ' wherein _Ab=h ' _{Ab, b}-h ' _{Ab, s}

In the 4th step, calculate S _L, S _cAnd S _H, and R _TAnd R _C

$S_L=1+\frac{0.015\×{(\stackrel{\‾}{L^{\′}}-50)}^2}{\sqrt{20+{(\stackrel{\‾}{L^{\′}}-50)}^2}}$ $S_C=1+0.045\×\stackrel{\‾}{C_{\mathrm{ab}}^{\′}}$ $S_H=1+0.015\×\stackrel{\‾}{C_{\mathrm{ab}}^{\′}}\×T$

Wherein,

$T=1-0.17\×\cos (\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}-{30}^0)+0.24\×\cos \left(2\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}\right)+0.32\×\cos (3\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}+6^0)-0.2\×\cos (4\stackrel{\‾}{h_{\mathrm{ab}}^{\′}}-{63}^0)$

The L ', the h ' that divide two colors calculating _AbArithmetic mean

R _T＝-sin(2Δθ)×R _C

Wherein, It is the rotation angle by the tone decision for

$R_C=2\sqrt{\frac{{\stackrel{\‾}{C}}_{\mathrm{Ab}}^7}{{\stackrel{\‾}{C}}_{\mathrm{Ab}}^7+{25}^7}},$ It is the rotation amplitude that changes according to chroma;

The 5th step, K _L, K _CAnd K _HSelection

K _L＝K _C＝K _H＝l；

In the 6th step, calculate aberration with formula (1);

4) evaluation of prepared slices of Chinese crude drugs appearance luster

Repeating step 2 and step 3 obtain the aberration of many batches of tested prepared slices of Chinese crude drugs appearance luster, confirm the aberration acceptable ranges.

3. a kind of method that detects prepared slices of Chinese crude drugs appearance luster according to claim 1 and 2 is characterized in that: said light source D ₆₅Be made up of incandescent lamp and fluorescent light, wherein the intensity of incandescent lamp accounts for 60-80%, remains the intensity into fluorescent light.

4. a kind of method that detects prepared slices of Chinese crude drugs appearance luster according to claim 1 and 2, it is characterized in that: the pixel of said CCD camera is greater than 5,000,000.