CN101792718A - Fungus strain for deteriorating biological plastics and use thereof - Google Patents
Fungus strain for deteriorating biological plastics and use thereof Download PDFInfo
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Abstract
The invention discloses a fungus strain for deteriorating biological plastics and use thereof. The microbial preservation number of the fungus strain (Bionectria ochroleuca) is CGMCC NO.3470. The fungus strain has wide adaptation scope for natural environmental conditions of temperature, pH value, and the like, and has the capacity for deteriorating the biological plastics in the pH range of 4 to 11. The fungus strain is derived from ordinary soil, is easy to culture and store, and can be fermented for culturing by utilizing soybean oil, glycerin, or glucose as replaced carbon sources of PBS (Polybutadiene-styrene). The fungus strain can quickly deteriorate the biological plastics of PBS, PBSA, and the like and wastes thereof, promote the use of biodegradable plastic and reduce white pollution, and is beneficial to environmental protection.
Description
Technical field
The present invention relates to a fungus strains, the Bionectria that relates in particular to biological plasticss such as a strain degraded PBS, PBSA belongs to fungal bacterial strain.
Background technology
The production of plastics and application have brought huge convenience and economic interests to people, world's plastics gross annual output amount is above 1.7 hundred million tons now, and annual speed with 2,500 ten thousand tons in environment, accumulate (the lucky equality of history. China's degradable plastics is studied and production status. the Shanghai plastics, 2006,6 (2): 4～8).China is one of ten big plastics production and consumption states in the world, the plastic refuse that annual various Plastic Packaging Materialss, agricultural mulching, pot for growing seedlings, preservative film, disposable daily use Sundry goods and medical material etc. are difficult to recycle reaches more than 3,500 ten thousand tons, thereby the waste or used plastics in the environment because of its nature be difficult for to decompose cause problem of environmental pollution of being on the rise (Chen Xirong. degradative plastics: the most important thing of plastic development. China packaging industry, 2006, (5): 14～17; Tang Saizhen etc. promote the thinking of biodegradable plastic practicalization. new material industry, 2005, (7): 53～59; Zhao Hongyu etc. biodegradable plastic and Sustainable development. Chinese plastics, 2006, (11): 14～20).
" plastic limit " that issuing and implementation played on June 1st, 2008 in the plastic limit of countries in the world and China further shown the attention of the environmental pollution that people cause plastics, but these measures still can not fundamentally solve " white pollution " problem.At present, the research of the substitute-biodegradable plastic of conventional plastic (as PLA, PHA, PBS, PBSA etc.) has become the research focus of 21 century countries in the world plastic working industry.China has built up the PBS production line of producing 20000 tons per year in May, 2007, and its output will rise year by year.As seen, the research and development of biodegradable plastic and the central topic of using the new technology material that has become 21 century.
But, along with the continuous increase of biodegradable plastic usage quantity, problem of environmental pollution new, that can not be ignored has appearred.1. synthetic plastics and biodegradable plastic are separated separately and reclaim, even also be extremely difficult in the Xian Jin garbage retrieving system in the world; 2. the PLA class material as the biodegradable plastic main component is difficult to decompose in fact at normal temperatures, performance is highly stable in normal storage and use, normal temperature decomposes in physical environment still needs for a long time, only just can decompose when also assurance contacts with the microorganism of specific (under conditions such as compost) in certain humidity.Therefore, say still there is not complete contaminated solution problem in some sense; 3. agricultural biological degradable plastics such as plastic sheeting for farm use can not decompose then or after the stubble crop is used immediately, causes sowing at second stubble crop, grows seedlings, has a strong impact on during farming the use of agricultural machinery and implement and follow-up production; More or less some difficult degradation compositions of existence in the composition of 4. present various degradable plasticss; 5. biodegradable plastic is for the influence of the microbial ecological in environment system and whether can secondary pollution still unclear.The foreign study result shows that microorganism is one of key factor that solves the biological plastics degraded.
The existing abroad report of enzyme liberating PLA that from microorganism, extracts or the research document of PBS or patent, and the report microorganism is as the sole carbon source utilization with PLA or PBS.But up to the present, fungi, especially Bionectria belong to fungal bacterial strain degraded PBS and utilize other carbon sources to produce degrading enzymes degraded PBS and the report that microorganism strains or fermented liquid are applied directly to degraded in soil PBS plastic film for agricultural use the like waste is not seen both at home and abroad.
Summary of the invention
One of purpose of the present invention is to be difficult to the problem of degrading in the short period of time under field conditions (factors) and the effectively Bionectria genus fungal bacterial strain of deteriorating biological plastics (especially poly butylene succinate (PBS)) of a strain is provided in order to solve biodegradable plastic PBS.
Two of purpose of the present invention is purposes that above-mentioned fungal bacterial strain is used for deteriorating biological plastics (especially poly butylene succinate) aspect.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
Red shell bacterium (Bionectria ochroleuca BFM-X1) is given birth in a strain of the present invention effectively fungal bacterial strain light color of deteriorating biological plastics, and its microbial preservation number is: CGMCCNO.3470; The classification name is: Bionectria ochroleuca; The preservation time: on December 10th, 2009; Preservation address: BeiChen West Road, Chaoyang District, BeiJing City, Institute of Microorganism, Academia Sinica; Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC).
The morphological specificity of fungal bacterial strain of the present invention: bright ivory white of bacterium colony initial stage on the PDA substratum, mycelia is thin, be close on the substratum, the late stage of culture bacterium colony becomes the rape Huang in substratum back side color by the look of cultivating the initial stage, gradually become the traffic Huang, finally become broom yellow's (with reference to RALK7 international standard colour atla), cultivating down at 28 ℃ promptly had generation of conidium in 3 days.The elongated upright general about 20-30 μ m of conidiophore, the verticillate conidium stigma in top, it is agglomerating that gathering is given birth on the conidium top, the more smooth nothing of outer wall every, oval to nearly sausage shape, wall is thin, colourless, big or small 4.2-7.3 * 2.3-4.0 μ m, average 5.35 * 2.60 μ m.
The separating screening method of bacterial strain Bionectria ochroleuca BFM-X1 of the present invention: get fresh forest soil 10g in the beaker that fills the 100ml sterilized water, beaker is placed on the magnetic stirring apparatus leaves standstill 10min after stirring 10min, suspension is diluted successively with 10 times of gradient dilution methods obtain 10
-5, draw 0.2ml above-mentioned 10
-5The soil diluent is put into 28 ℃ of constant temperature culture in the incubator after smearing evenly with glass spatula on the minimal medium that contains PBS emulsion (composition the is following) flat board that is added with paraxin.Fungal colony occurs after 2-3 days, transparent circle appears in some periphery of bacterial colonies simultaneously.After cultivating 72h, select transparent circle at the bacterium colony more than the 2cm and purifying on new PDA flat board, be inoculated in the central authorities of the inorganic salt plate culture medium that contains PBS emulsion when treating bacterium colony length about picking mycelia piece 2mm to diameter 2cm, and be that centrosymmetry is put into 4 of the PBS films of the equal size that sterilising treatment crosses (20mm * 20mm), the space is 2mm with it.After will handling ware and placing incubator constant temperature secretly to cultivate 10d, find not have the mycelia of the bacterial strain of degradation function not expand and grow into PBS film surface, and the bacterial strain with degradation function can expand to the film surface from the inoculation point, mycelial growth is in great numbers.At this moment, the PBS diaphragm below the mycelia has been degraded or only surplus degraded relic in latter stage.
It is as follows that the screening of bacterial strain Bionectria ochroleuca BFM-X1 of the present invention and degradation characteristic are measured the substratum that adopts:
The screening and culturing based component: 0.01g ferrous sulfate (sterilization separately), 0.5g Repone K, 2g SODIUMNITRATE, 1g dipotassium hydrogen phosphate, 0.5g sal epsom, 0.0005g manganous sulfate, paraxin 0.05g, agar 15g and quantitative PBS emulsion, it is 1000ml that deionized water is regulated cumulative volume.The sterilization postcooling falls dull and stereotyped stand-by.
Fermention medium: do not add agar and paraxin, all the other compositions are the same.
When measuring, carbon source replaces PBS emulsion in solid or the liquid nutrient medium respectively with 1% glucose (W/V), 1% soybean oil (V/V) or 1% glycerine (V/V) etc.
On reciprocating type shaking table (120rpm), the bottled amount of fermention medium is the 250ml/500ml triangular flask, and inoculum size is that 5%, 28 ℃ of following ferment at constant temperature cultivated for 1 week.
The cultural characters that belongs to the bacterial strain Bionectria ochroleuca BFM-X1 of fungi of the present invention:
(1) culture temperature: 5 ℃-37 ℃, 25 ℃-35 ℃ of optimum temperature ranges;
(2) cultivate pH value 4-11.But growth in thickness, aerial hyphae, the best pH of sporulation quantity etc. has nothing in common with each other.
(3) this bacterial strain aerial hyphae on the MEA substratum is undeveloped, and mycelia is very thin, have certain transparent texture, tightly be attached on the substratum, and the mycelia color is similar to the substratum background color, and to be shallow chestnut yellowish pink, and back side pigment is not obvious.Aerial hyphae tubbiness, abundant, tight on the YES substratum, oyster white general again light red, similar cheese has therefrom radial wrinkle around the mind-set; The bottom pigment is that beige is to drabon look gradually dark from outside to inside, and the pleat line that highlights is like the chrysanthemum inflorescence.Between MEA and YES, mycelia interweaves, and to present suede cotton-shaped aerial hyphae on the CYA substratum, but consolidation is attached at and is beige on the substratum, and radial wrinkle around the mind-set is also arranged therefrom, and back side pigment is light orange red.
Extract the rDNA of bacterial strain of the present invention simultaneously and measure the regional gene order (fungi universal primer: ITS1 5 '-TCCGTAGGTGAACCTGCGG-3 ' and ITS4 5 '-TCCTCCGCTTATTGATATGC-3 ') of ITS (comprising 5.8S), this bacterial strain ITS regional gene sequence is carried out the sequence homology comparison at the NCBI gene pool, and the homology of discovery and Bionectriaochroleuca is all more than 99%.Morphological specificity (the Schroers HJ.2001 that contrast Bionectria belongs to; Zhuang WY 2007), combining form is learned and molecular biology method identifies that bacterial strain BFM-X1 is that Bionectria belongs to bacterial strain, confirms the make a living red shell of light color life (Bionectria ochroleuca (Schwein.) the Schroers ﹠amp of neocosmospora of bacterial strain of the present invention; Samuels) fungi.
In addition, the fungal bacterial strain with belonging to of following characteristic of living neocosmospora (Bionectria) also has the characteristic of efficient degradation poly butylene succinate (PBS), and these bacterial strains also should belong within protection scope of the present invention:
(1) available carbon source material: soybean oil, glycerine, glucose and PBS; Available carbon source concentration scope is 1wt%～10wt%.
(2) indoor degradation temperature scope: 15 ℃～38 ℃, 25 ℃～35 ℃ of the suitableeest degradation temperatures;
(3) degraded pH value 4～11, the down best degraded pH of specified conditions scope is 4～8;
Fungal bacterial strain of the present invention also comprises mutant, varient or its various meta-bolitess, the derivative of bacterial strain Bionectria ochroleuca BFM-X1 of the energy efficient degradation PBS of the above bacterial strain.
Fungal bacterial strain of the present invention obtains culture or nutrient solution by following culture medium culturing, be used for degradation treatment: liquid culture: 0.001% ferrous sulfate, 0.05% Repone K, 0.2% SODIUMNITRATE, 0.1% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.0005% manganous sulfate, 1%PBS emulsion are adjusted to required cumulative volume than row with deionized water by above-mentioned.Add agar by 1.5% during solid culture.Autoclaving is stand-by.In addition, the carbon source material that is utilized in the above-mentioned substratum can be used soybean oil, glycerine, and glucose replaces; The carbon source concentration scope of being utilized is 1wt%～10wt%.During liquid culture bacterial strain of the present invention inserted by 5% inoculum size and be equipped with in the 500ml triangular flask of 250ml fermention medium,, carry out fermentation culture under 28 ℃ at reciprocating type shaking table (120rpm).Cultivate promptly can be used for the degrading processing of PBS of fermented liquid after 7～10 days.
Bacterial strain of the present invention degradation efficiency in a short time is very remarkable, temperature on average be lower than use bacterial strain fermentation liquor of the present invention under 20 ℃ of conditions and handle soil after, the degradation rate of PBS film in the time of 70 days that is embedded in the soil is 23.02%, and the following 70 days degradation rate of manually preserving moisture reaches 70.84%; Be higher than under 28 ℃ of conditions at monthly mean temperature, be embedded in PBS film in the soil in the time of 70 days degradation rate up to 75.44%, and the following 70 days degradation rate of situation of manually preserving moisture degraded fully when reaching 96.73%, the 75 day and checking can not find PBS plastics film relic in the soil.Behind the fermentation liquor treatment soil of control soil temperature and humidity condition in laboratory with this bacterial strain, be embedded in PBS plastics film in the soil in the time of 30 days degradation rate up to 65.48%.
Bacterial strain of the present invention except the PBS that can degrade, the poly-succinic/hexanodioic acid-butanediol ester (PBSA) of can also effectively degrading, and degradation time is shorter, efficient is higher.
Bacterial strain Bionectria ochroleuca BFM-X1 of the present invention has the ability of degraded PBS to the wide accommodation of natural environmental conditions such as temperature, pH in the pH4-11 scope.Bacterium source of the present invention is cultivated easily and is preserved in normal soil, can utilize soybean oil, glycerine, or glucose carries out fermentation culture as the alternative carbon source material of PBS to it.Bacterial strain of the present invention can be accelerated the degraded of biological plastics such as PBS, PBSA and waste thereof, promotes the use of biodegradable plastic, reduces white pollution, helps environment protection.
Description of drawings
Fig. 1 is fungal bacterial strain screening of the present invention and degradation function technological line figure.
Fig. 2 utilizes the degradation rate of different carbon sources to the PBS film for bacterial strain Bionectria ochroleuca BFM-X1 of the present invention.
Fig. 3 for bacterial strain Bionectria ochroleuca BFM-X1 of the present invention to the PBS film relic scintigram in kind of degrading in time.
Fig. 4 cultivates the degradation efficiency of 18d to the PBS film for bacterial strain Bionectria ochroleuca BFM-X1 of the present invention under certain carbon source differing temps.
Fig. 5 be under the condition of different pH bacterial strain Bionectria ochroleucaBFM-X1 of the present invention to the degradation efficiency of PBS film.
Fig. 6 is for behind the fermentation liquor treatment soil of bacterial strain of the present invention, PBS film degradation efficient in the following 30 days soil of indoor control soil temperature and humidity condition.
After Fig. 7 (a) is the usefulness fermentation liquor treatment soil of bacterial strain of the present invention, PBS film degradation rate in 28 ℃ of following outdoor soil of month samming.
Fig. 8 (b) is with behind the fermentation liquor treatment soil of bacterial strain of the present invention PBS film degradation rate in the outdoor soil under a month samming is lower than 20 ℃.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall within the scope of protection of the present invention the details of technical solution of the present invention and form.
Embodiment 1
The screening method of the bacterial strain Bionectria ochroleuca BFM-X1 of efficient degradation PBS of the present invention:
(1) soil of isolated strains picks up under the Changji state Xinjiang Agricultural Univ Western Hills test forest farm spruce forest.Get fresh soil 10g in the beaker that fills the 100ml sterilized water, beaker is placed on the magnetic stirring apparatus leaves standstill 10min after stirring 10min, dilute successively with 10 times of gradient dilution methods and obtain 10
-5Diluent is drawn 0.2ml above-mentioned 10
-5The soil diluent is put into 28 ℃ of constant temperature culture in the incubator after smearing evenly with glass spatula on the minimal medium flat board of the 1%PBS emulsion that is added with paraxin.Minimal medium: 0.001% ferrous sulfate (must sterilize separately, be added in the total amount during use), 0.05% Repone K, 0.2% SODIUMNITRATE, 0.1% dipotassium hydrogen phosphate, 0.05% sal epsom, 0.0005% manganous sulfate and 1.5% agar.
(2) fungal colony occurs after 2-3 days, transparent circle appears in some periphery of bacterial colonies simultaneously.After cultivating 72h, select transparent circle at the bacterium colony more than the 2cm and purifying on new PDA flat board.
(3) be inoculated in the dull and stereotyped central authorities of the minimal medium of the above-mentioned PBS of containing emulsion about the mycelia piece 2mm of picking purifying bacterial strain, and be 4 of the PBS films that centrosymmetry is put into 2cm * 2cm that 70% alcohol disinfecting handled with it, the space is 2mm.In the time of will handling ware and place the dark 5～7d of cultivation of thermostat container, there is the bacterial strain of degradation function to expand to the film surface from the inoculation point, mycelial growth is in great numbers on face, PBS diaphragm below the mycelia has been degraded or only surplus degraded relic in latter stage at this moment, do not have the bacterial strain of degradation capability only in the gap of four films, to grow to the PBS film, can not expand to the film surface.
(4) aseptic technique picking mycelia piece from the degraded relic is transferred on the PDA flat board, and purifying is also confirmed degradation property, obtains efficient degrading bacterial strain Bionectria ochroleuca BFM-X1.
Test example 1
Be taken at the Bionectria ochroleuca BFM-X1 bacterium piece 2mm that cultivated on the PDA substratum 7 days, be inoculated into the central authorities that ware is handled in each test, and be 4 of the PBS films that centrosymmetry is put into 2cm * 2cm that 70% alcohol disinfecting handled with it, the space is 2mm.Temperature relation is measured ware (establish a processing every 5 ℃ between 10 ℃～40 ℃, establish 7 thermogrades altogether), and constant temperature is secretly cultivated 18d under each Temperature Treatment respectively.All the other each tests are handled ware and are placed 28 ℃ of constant temperature secretly to cultivate 18d, and control treatment 3 wares are all established in each test.Contrast ware except that not connecing Bionectria ochroleuca BFM-X1 bacterial strain, all the other same test group.Cultivate and take each film of testing the processing ware after finishing respectively gently off, blot residual film surface-moisture, scan remaining PBS film image respectively with filter paper with sterilized water gently flushing repeatedly.Be used for determining that degradation rate handles parallel 10 wares that connect with the test of timing relationship, from cultivating second day, the next day, takes out a ware and takes 4 films gently off, flushing, scans remaining PBS film image.Use ImageJ 1.38x software processes scanning picture at last, calculate the area that the film loss is handled in each test.The decrement of its area is and respectively handles the degradation rate of bacterial strain to it.Calculation formula is: area sum/4 * 100% of degradation rate (%)=film loss.Correlation test the results are shown in Table 1 and Fig. 2～5.
Fig. 2 utilizes the degradation rate of different carbon sources to the PBS film for bacterial strain Bionectria ochroleuca BFM-X1.Fungal bacterial strain of the present invention all can produce Degradation, degradation effect significant difference to the PBS film with certain density glycerine, soybean oil, glucose, PBS emulsion on respectively as the substratum of sole carbon source when specified temp is secretly cultivated 18d.Fig. 3 for bacterial strain Bionectria ochroleuca BFM-X1 of the present invention to the PBS film relic scintigram in kind of degrading in time.Under certain carbon source specified temp, degradation rate is approaching very during 18d.Degrade fully in the time of the 20th day, do not had film solid relic in the ware.Fig. 4 cultivates the degradation efficiency of 18d to the PBS film for bacterial strain Bionectria ochroleuca BFM-X1 of the present invention under certain carbon source differing temps.Fig. 5 be under the condition of different pH bacterial strain Bionectria ochroleuca BFM-X1 of the present invention to the degradation efficiency of PBS film.Bacterial strain of the present invention is when pH4, and 14d can degrade PBS film 100% fully under specified temp and certain carbon source and the carbon source concentration condition.The pH scope of degraded is 4-11.
Bacterial strain BFM-X1 of the present invention is to the degradation rate (%) of PBS film on the different emulsion concentration of table 1 substratum
The PBS film degradation was tested after the indoor bacterial strain fermentation liquor of the present invention of test example 2 experiments was handled soil.
The soil of sterilizing and stirring with bacterial strain fermentation liquor of the present invention that earlier shop 3cm is thick in test board (by every 1kg soil with 150ml fermented liquid stir process) is lay (in advance each sheet PBS film being carried out weighing) PBS film (4cm * 4cm) according to the order of sequence then, cover fermentation liquor treatment soil 3cm again, cover gauze above and preserve moisture.Every other day spray quantitative aqua sterilisa and keep certain humidity, the temperature control constant temperature culture.Sampling (get 2 parallel) every other day under the situation of not destroying the material surface structure, is carefully cleaned surperficial earth, dries, and weighs, and utilizes the weight weight-loss method to weigh the material degradation rate.Control group is handled and is not sprayed bacterial strain fermentation liquor of the present invention, and all the other are the same.Test-results is seen Fig. 6.Test-results as seen, laboratory control soil temperature and humidity condition is with behind the fermentation liquor treatment soil of bacterial strain of the present invention, be embedded in PBS film in the soil in the time of 30 days degradation rate up to 65.48%.
Test example 3 usefulness bacterial strain fermentation liquor of the present invention is in the degraded test of outdoor natural nursery lot to the PBS film.
Earlier wholely, do the ridge that 30cm is wide, 80cm is long, distance between the ridges 25cm, the lay that (before test, each sheet PBS material carried out weighing) according to the order of sequence on every ridge 2 row PBS films (8cm * 8cm), diaphragm spacing 2cm, spread one deck thin soil on the film, treatment group is evenly sprayed 250ml with watering can and is secretly cultivated (the strain culturing fermented liquid of the present invention that reciprocating type shaking table (120rpm) 7～10d) obtains, earthing 5cm again through 28 ℃ of constant temperature.Bacterial strain fermentation liquor of the present invention is not sprayed on the control group ridge, directly earthing 5cm.In order to grasp the degraded influence of humidity to the PBS film, artificial quantitatively water spray is preserved moisture and is not preserved moisture and keeps two groups of state of nature next day that treatment group and control group being established on the bed of ridge again respectively.The sampling in 5 days of every interval under the situation of not destroying the material surface structure, is carefully cleaned surperficial earth, is dried, weighs, and utilizes the weight weight-loss method to weigh degradation rate.The per-cent that the variation of testing front and back weight is accounted for original weight is considered as degradation rate.Get 2 parallel samples at every turn.
The calculating of degradation rate: degradation rate=[(original sample quality-degraded back sample mass)/original sample quality] * 100%.
Correlation test the results are shown in Figure 7 (a) and Fig. 8 (b).Be higher than under 28 ℃ of conditions at monthly mean temperature, the PBS film that is embedded in the soil is 80.78% when degradation rate was up to 75.44%, 75 day in the time of 70 days; And the following 70 days degradation rate of situation of manually preserving moisture degraded fully when reaching 96.73%, the 75 day and checking can not find PBS plastics film relic (Fig. 7 (a)) in the soil.Be lower than at temperature on average use bacterial strain fermentation liquor of the present invention under 20 ℃ the condition and handle soil after, the PBS film that is embedded in the soil is 42.72% when degradation rate is 23.02%, 75 day in the time of 70 days; The following 70 days degradation rate of the condition of manually preserving moisture reaches 50.84%; Reached 70.65% (Fig. 8 (b)) in the time of 75 days.
Claims (10)
1. the fungal bacterial strain of a strain deteriorating biological plastics (Bionectriaochroleuca), its microbial preservation number are: CGMCC NO.3470.
2. the mutant of the described fungal bacterial strain of claim 1, varient or derivatives thereof.
3. according to mutant, the varient or derivatives thereof of the described fungal bacterial strain of claim 2, it is characterized in that: the carbon source material that it utilized is selected from soybean oil, glycerine, glucose or biological plastics poly butylene succinate; The carbon source concentration scope of being utilized is 1wt%～10wt%.
4. strain culture or the fermentation culture that obtains by the described fungal bacterial strain of claim 1.
5. by the mutant of claim 2 or 3 described fungal bacterial strains, strain culture or the fermentation culture that the varient or derivatives thereof obtains.
6. the purposes of the described fungal bacterial strain of claim 1 in deteriorating biological plastics.
7. according to the described purposes of claim 6, it is characterized in that, comprising: the described fungal bacterial strain of claim 1 is cultivated, obtained strain culture or fermentation culture; With resultant strain culture or fermentation culture deteriorating biological plastics; Wherein, microorganism culturing and deteriorating biological plastics environment PH are controlled at 4-11.
8. the mutant of claim 2 or 3 described fungal bacterial strains, the purposes of varient or derivatives thereof in deteriorating biological plastics.
9. according to claim 4 or 5 described strain cultures or the purposes of fermentation culture in deteriorating biological plastics.
10. according to claim 6,8 or 9 described purposes, it is characterized in that described biological plastics comprises poly butylene succinate or poly-succinic/hexanodioic acid-butanediol ester.
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| CN103613782A (en) * | 2013-11-28 | 2014-03-05 | 南京工程学院 | Waste plastic recycling method based on mycelia |
| CN107354101A (en) * | 2017-07-10 | 2017-11-17 | 广东省农业科学院农业资源与环境研究所 | The Penicillium citrinum fungi of one plant of degradable film and its application |
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| CN107894343A (en) * | 2017-10-20 | 2018-04-10 | 广东省农业科学院农业资源与环境研究所 | A kind of recovery method for plastic sheeting in liquid and soil |
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| CN101792718B (en) | 2011-11-16 |
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