CN101791431B - Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue - Google Patents
Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue Download PDFInfo
- Publication number
- CN101791431B CN101791431B CN2010101055732A CN201010105573A CN101791431B CN 101791431 B CN101791431 B CN 101791431B CN 2010101055732 A CN2010101055732 A CN 2010101055732A CN 201010105573 A CN201010105573 A CN 201010105573A CN 101791431 B CN101791431 B CN 101791431B
- Authority
- CN
- China
- Prior art keywords
- periodontal
- biodegradable
- diaphragm
- regeneration
- periodontal tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Materials For Medical Uses (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a preparation method of a biodegradable diaphragm for promoting regeneration of periodontal tissue, belonging to the field of biological regeneration medicine. In the invention, polylactic acid, polycaprolactone, polylactic acid-glycollic acid copolymer, collagen, modified chitosan and other biodegradable synthetic polyester polymers or natural biodegradable high molecular materials are used as raw materials to prepare a thin-film material with a nano structure through the electrostatic spinning process, and then, various growth factors are loaded on the thin-film material through the supermolecule loading technology. The diaphragm for reparative regeneration of the periodontal tissue, which is prepared by the method of the invention, has good biocompatibility and can realize the purposes of inhibiting inflammation and inducing regeneration of the periodontal tissue through the controlled release of the loaded growth factors.
Description
Technical field
The invention belongs to the bio-regeneration medical domain, be specifically related to a kind of method for preparing of biodegradable diaphragm for promoting regeneration of periodontal tissue.
Background technology
Periodontal is one of human the most common, the most multiple disease, and wherein modal is exactly periodontitis.The significant pathological change of periodontal is the periodontal support disorganization: frontal resorption, destroy and absorb with in various degree cementum attached to the connective tissue on the sclerotin, will cause odontoseisis to come off if can not get efficacious therapy.In the microbial environment in oral cavity, the main composition part of antibacterial, the lipopolysaccharide of derived from gram-negative bacteria can cause irritating bone forfeiture of similar periodontal disease.LPS reacts the expression [1-3] that stimulates interleukin-1 ' beta ', tumor necrosis factor-alpha, interleukin-6 and RNAKL through activating inherent immunity.The production process of inflammatory factor is by the signal path of enzyme induction and the activatory result of transforming factor.Lipopolysaccharide causes this process through associating CD14 and Toll appearance receptor, and that play a major role is Toll-2 and Toll-4 [4-6].No matter be that which kind of Toll appearance receptor works, LPS can increase RANKL, IL-1, PGE
2With TNF-α [7].The various factors can promote osteoclast activity, propagation and differentiation.In addition, activatory mononuclear cell, macrophage and fibroblast can produce and destroy the IL-1 of periodontal tissue, PGE
2, cytokine such as TNF-α, with respect to the periodontal tissue of health, the amount of these cytokines has at periodontal destruction place increases [8-14] significantly.These cytokines reflect in the destructive process of present periodontal tissue, and its product comprises the enzyme of participating in inflammatory reaction and the matrix metalloproteinase and the prostaglandin of mediated factor.In addition, the antiinflammatory factor is passed through RNAKL-dependency and non-dependency line path activation osteoclast directly or indirectly, thereby has caused irreversible bone destruction [15,16].Having high degree of specificity recognition system inherent immunity is the first line of defence [17] of defence pathogen invasion.Inherent immunocyte comprises that macrophage and BMDC can express a series of Toll-appearance receptor, and they can be united each other and form high degree of specificity and express the molecular system that is associated.Importantly, the lipopolysaccharide that extracts from gram negative bacteria is through the serious inflammatory reaction of combination initiation of CD14 and Toll appearance receptor (mainly being TLR-2 and TLR-4 receptor).Behind the lipopolysaccharide identification TLR-4, the cell-mediated production of cytokines of inherent immunity, cause a series of meticulous signal transduction pathway after, the adaptive immunoreation [5,6] that the host promptly produces.Periodontal tissue takes place acutely inflamed the time, and the expression of TLR-2 and TLR-4 can increase, and has shown that the increase of these receptors can influence cytokine expression ability [18].IRAK, the activation immediately of TRAF6 path behind the TLR-4 path activation MyD88-dependency path, the nuclear factor (NF-κ B) that finally has the cytokine induction function is activated.And the TRAF6 path replenishes and/or needs cytokines mRNA to transcribe in the process of circulation with the stable various paths of mRNA different protein receptors, ERK1/2 for example, and JNK and p38 are essential [19,20].
As a rule, the key that obtains paradenlal tissue regeneration clinically is the use of barrier film, and the effect of this barrier film is to organize the field planting at the root face of gingiva and epithelial cell, and leaves certain space [21] to the regeneration of periodontal membrane tissue.The theory of this guided bone tissue regeneration helps the reconstruction of tissue bond epithelium, but it can not guide the propagation [22] that can promote the alveolar bone regenerative cell.Seeking out paradenlal tissue regeneration must join together some The key factor.Bone marrow stroma stem cell and periodontal fibroblast are two kinds of cells that can make the paradenlal tissue regeneration of forfeiture.In addition, needing blood supplies to come for tissue forms nutrient source and template [23,24] to be provided with the support of certain mechanical strength.We have used the method for some paradenlal tissue regenerations and periodontal tissue's engineering to overcome the defective of conventional method.The method of cell source, support manufacturing method and somatomedin and gene is improved.Periodontal membrane can be that basic methods realizes with the timbering material through some with the regeneration that other dental branches are held tissue.In conjunction with in the pig body, having induced the formation of tooth, and produce well-regulated periodontal tissue 33% success rate is arranged from the gel-chrondroitin-hyaluronidase support of body tooth bud cell.In another one research, people's periodontal ligament cell is implanted in to have produced on the hydroxyapatite/chitosan stent of porous nano microcrystalline and interconnects ingrown microtubule tissue.Chitosan/collagen scaffold and TGF-1 gene and people's periodontal ligament cell have combined to obtain the regeneration of ideal periodontal membrane tissue.This support is compared people's periodontal ligament cell with the method for TGF-1L associating and has been obtained propagation [25] significantly with simple support.Recently some research steerings the biological activity growth factor release is increased the research of the formation of periodontal tissue, the release that this substrate can the refined control factor comes for the recovery of periodontal tissue's damage method to be provided.For example, the PDGF that discharges in the tricalcium phosphate can promote to have the generation [26] of periodontal local damage patient VEGF.The release of IGF-1 has caused new bone tissue, cementum and pericemental new life in external dextran-gel micro-ball.The effect of somatomedin in periodontal reparative regeneration process caused widely to be paid close attention to, because spontaneous growth factor source is limited, therefore effect and the function of a large amount of experiments in the research exogenous growth factor is arranged.Research shows that multiple somatomedin can regulate the biosynthesis of the propagation of periodontal relevant cell, chemotactic, differentiation and extracellular matrix, can obviously promote the regeneration and the reparation of periodontal tissue.Yet, exogenous growth factor half-life weak point, local use is very fast diluted, and inactivation only just can make somatomedin continue efficiently to play a role by suitable carriers with metabolism or because of the effect of body endoenzyme, promotes tissue regeneration better.
In sum; In the report of existing document, as yet not relevant for the combination Biodegradable nano structure diaphragm described in the present invention, and with the mode of somatomedin controlled release to periodontal tissue's inflammation and regeneration; Segmentation suppresses and induces, to realize the correlational study report of promoting regeneration of periodontal tissue.
List of references
1.Nakashima?T，Kobayashi?Y，Yamasaki?S，Kawakami?A，Eguchi?K，Sasaki?H，Sakai?H.Protein?expressionand?functional?difference?of?membrane-bound?and?soluble?receptor?activator?of?NF-kappaB?ligand：modulation?of?the?expression?by?osteotropic?factors?and?cytokines.Biochem?Biophys?Res?Commun?2000；275(3)：768-75.
2.Jiang?Y，Mehta?CK，Hsu?TY，Alsulaimani?FF.Bacteria?induce?osteoclastogenesis?via?anosteoblast-independent?pathway.Infect?Immun?2002；70(6)：3143-8.
3.Kikuchi?T，Yoshikai?Y，Miyoshi?J，Katsuki?M，Musikacharoen?T，Mitani?A，Tanaka?S，Noguchi?T，Matsuguchi?T.Cot/Tpl2?is?essential?for?RANKL?induction?by?lipid?A?in?osteoblasts.J?Dent?Res?2003；82(7)：546-50.
4.Yang?H，Young?DW，Gusovsky?F，Chow?JC.Cellular?events?mediated?by?lipopolysaccharide-stimulatedtoll-like?receptor?4.MD-2?is?required?for?activation?of?mitogen-activated?protein?kinases?and?Elk-1.J?BiolChem?2000；275(27)：20861-6.
5.Kirschning?CJ，Bauer?S.Toll-like?receptors：cellular?signal?transducers?for?exogenous?molecular?patternscausing?immune?responses.Int?J?Med?Microbiol?2001；291(4)：251-60.
6.Kirschning?CJ，Wesche?H，Merrill?Ayres?T，Rothe?M.Human?toll-like?receptor?2?confers?responsiveness?tobacterial?lipopolysaccharide.J?Exp?Med?1998；188(11)：2091-7.
7.Roux?S，Orcel?P.Bone?loss.Factors?that?regulate?osteoclast?differentiation：an?update.Arthritis?Res?2000；2(6)：451-456.
8.Reddi?K，Wilson?M，Nair?S，Poole?S，Henderson?B.Comparison?of?the?pro-inflammatorycytokine-stimulating?activity?of?the?surface-associated?proteins?of?periodontopathic?bacteria.J?PeriodontalRes?1996；31(2)：120-30.
9.Lee?HJ，Kang?IK，Chung?CP，Choi?SM.The?subgingival?microflora?and?gingival?crevicular?fluid?cytokinesin?refractory?periodontitis.J?Clin?Periodontol?1995；22(11)：885-90.
10.Ejeil?AL，Gaultier?F，Igondjo-Tchen?S，Senni?K，Pellat?B，Godeau?G，Gogly?B.Are?cytokines?linked?tocollagen?breakdown?during?periodontal?disease?progression?J?Periodontol?2003；74(2)：196-201.
11.Gamonal?J，Acevedo?A，Bascones?A，Jorge?O，Silva?A.Levels?of?interleukin-1?beta，-8，and-10?andRANTES?in?gingival?crevicular?fluid?and?cell?populations?in?adult?periodontitis?patients?and?the?effect?ofperiodontal?treatment.J?Periodontol?2000；71(10)：1535-45.
12.Geivelis?M，Turner?DW，Pederson?ED，Lamberts?BL.Measurements?of?interleukin-6?in?gingival?crevicularfluid?from?adults?with?destructive?periodontal?disease.J?Periodontol?1993；64(10)：980-3.
13.Gorska?R，Gregorek?H，Kowalski?J，Laskus-Perendyk?A，Syczewska?M，Madalinski?K.Relationshipbetween?clinical?parameters?and?cytokine?profiles?in?inflamed?gingival?tissue?and?serum?samples?frompatients?with?chronic?periodontitis.J?Clin?Periodontol?2003；30(12)：1046-52.
14.Stashenko?P，Jandinski?JJ，Fujiyoshi?P，Rynar?J，Socransky?SS.Tissue?levels?of?bone?resorptive?cytokinesin?periodontal?disease.J?Periodontol?1991；62(8)：504-9.
15.Graves?DT.The?potential?role?of?chemokines?and?inflammatory?cytokines?in?periodontal?diseaseprogression.Clin?Infect?Dis?1999；28(3)：482-90.
16.Graves?DT，Delima?AJ，Assuma?R，Amar?S，Oates?T，Cochran?D.Interleukin-1?and?tumor?necrosis?factorantagonists?inhibit?the?progression?of?inflammatory?cell?infiltration?toward?alveolar?bone?in?experimentalperiodontitis.J?Periodontol?1998；69(12)：1419-25.
17.Janeway?CA，Jr.，Medzhitov?R.Innate?immune?recognition.Annu?Rev?Immunol?2002；20：197-216.
18.Mori?Y，Yoshimura?A，Ukai?T，Lien?E，Espevik?T，Hara?Y.Immunohistochemical?localization?of?Toll-likereceptors?2?and?4?in?gingival?tissue?from?patients?with?periodontitis.Oral?Microbiol?Immunol?2003；18(1)：54-8.
19.An?H，Yu?Y，Zhang?M，Xu?H，Qi?R，Yan?X，Liu?S，Wang?W，Guo?Z，Guo?J，Qin?Z，Cao?X.Involvement?ofERK，p38?and?NF-kappaB?signal?transduction?in?regulation?of?TLR2，TLR4?and?TLR9?gene?expressioninduced?by?lipopolysaccharide?in?mouse?dendritic?cells.Immunology?2002；106(1)：38-45.
20.Schumann?RR，Pfeil?D，Lamping?N，Kirschning?C，Scherzinger?G，Schlag?P，Karawajew?L，Herrmann?F.Lipopolysaccharide?induces?the?rapid?tyrosine?phosphorylation?of?the?mitogen-activated?protein?kinaseserk-1?and?p38?in?cultured?human?vascular?endothelial?cells?requiring?the?presence?of?soluble?CD14.Blood1996；87(7)：2805-14.
21.Pan?SX，Li?Y，Feng?HL，Bai?W，Gu?YY.In?vitro?aging?of?mineralized?collagen-based?composite?as?guidedtissue?regeneration?membrane.Materials?Science?and?Engineering：C?2006；26(4)：724-729.
22.Owen?GR，Jackson?J，Chehroudi?B，Burt?H，Brunette?DM.A?PLGA?membrane?controlling?cell?behaviourfor?promoting?tissue?regeneration.Biomaterials?2005；26(35)：7447-56.
23.Kuo?TF，Huang?AT，Chang?HH，Lin?FH，Chen?ST，Chen?RS，Chou?CH，Lin?HC，Chiang?H，Chen?MH.Regeneration?of?dentin-pulp?complex?with?cementum?and?periodontal?ligament?formation?using?dental?budcells?in?gelatin-chondroitin-hyaluronan?tri-copolymer?scaffold?in?swine.J?Biomed?Mater?Res?A?2008；86(4)：1062-8.
24.Taba?M，Jr.，Jin?Q，Sugai?JV，Giannobile?WV.Current?concepts?in?periodontal?bioengineering.OrthodCraniofac?Res?2005；8(4)：292-302.
25.Zhang?Y-F，Cheng?X-R，Yun?C，Bin?S，Chen?X-H，Xu?D-X，Jin?K.Three-dimensionalNanohydroxyapatite/Chitosan?Scaffolds?as?Potential?Tissue?Engineered?Periodontal?Tissue.J?BiomaterAppl?2007；21(4)：333-349.
26.Cooke?JW，Sarment?DP，Whitesman?LA，Miller?SE，Jin?Q，Lynch?SE，Giannobile?WV.Effect?ofrhPDGF-BB?delivery?on?mediators?of?periodontal?wound?repair.Tissue?Eng?2006；12(6)：1441-50.
Summary of the invention
The object of the present invention is to provide a kind of method for preparing of biodegradable diaphragm for promoting regeneration of periodontal tissue.
The method for preparing of the biodegradable diaphragm for promoting regeneration of periodontal tissue that the present invention proposes; With one to several kinds in polylactic acid (PLA), polycaprolactone (PCL), polylactic acid-glycolic guanidine-acetic acid copolymer (PLGA), collagen (collagen) or the modification of chitosan (chitosan); Be dissolved in polarity, the volatile organic solvent; The concentration of control solution is 8-25wt%, through electrospinning process, makes the Biodegradable material thin film with nanostructured; Gained Biodegradable material thin film is adsorbed through the self assembly of supermolecule static; To be loaded on the macromolecule material film respectively with the somatomedin that promotes the periodontal osteanagenesis to the ERK/P38 cell pathway is inhibited; Through vacuum drying, behind the sterilization packaging, promptly get required product; Wherein: electrospinning process applies the high direct voltage scope and is: 0.5-50KV; Spinning head apart from the distance of gathering-device is: 5-100cm.
Among the present invention, be used for the polymer solution of electrostatic spinning, with the difference of the polymer that is added, polymeric blends, and the difference of molecular weight, the viscosity of solution system is also different.Specifically being advisable through electrostatic field gravitation filamentation.
Among the present invention, the mixture of one or more in said polarity, easy volatile solvent employing chloroform, dichloromethane, methanol, ethanol, propanol, hexafluoro propanol, ethyl acetate or the acetone.
Among the present invention; Said supermolecule load technology; Be meant static self-assembling method layer by layer, this method realizes the orderly assembling to biomacromolecule and polyelectrolyte based on the weak interaction force between macromole (electrostatic attraction, model Dehua power, hydrogen bond etc.); Except that biological activity that can keep biomacromolecule and space structure, also can on various material surfaces with complex space configuration, realize.
Among the present invention, said one type of somatomedin is inhibited micromolecular compound or an inhibitor of pair cell ERK/P38 cell pathway.Adopt: SB203580 (p38 cell pathway inhibitor), PD98059 (ERK cell pathway inhibitor), but be not restricted to this two example.
Among the present invention, micromolecular compound or the peptide sequence of said another kind of somatomedin for the periodontal osteanagenesis is had inducing action comprises like BMP (BMP), platelet-derived property somatomedin (PDGF), but is not restricted to this two example.
Advantage of the present invention: this biodegradable diaphragm for promoting regeneration of periodontal tissue has excellent biological compatibility and vivo degradation property; The more important thing is through to growth factor-loaded timesharing, typing controlled release, can realize that segmentation suppresses P38, ERK path (inflammation-inhibiting) and induces the activation paradenlal tissue regeneration.
Description of drawings
Scanning electron microscope (SEM) photo of the biodegradable diaphragm that Fig. 1 makes after for PCL electrostatic spinning among the embodiment 1.
Scanning electron microscope (SEM) photo of the biodegradable diaphragm that Fig. 2 makes after for PLGA electrostatic spinning among the embodiment 2.
Fig. 3 is PLGA: COLLAGEN=2 among the embodiment 3: scanning electron microscope (SEM) photo of the biodegradable diaphragm that makes behind 1 electrostatic spinning.
The specific embodiment
Further specify the present invention through embodiment below.
Embodiment 1
Employing PCL is a raw material, is dissolved in the chloroform, processes the solution that concentration is 15% (wt).This mixture solution is joined in the electrostatic spinning syringe through syringe pump control; The voltage of setting high tension generator is 10KV; Collecting distance is 15cm; Can obtain by the random thin film of forming of the superfine fibre of nanoscale diameter, then that this thin film is repeatedly for use after the final vacuum drying with deionized water wash.(PEI 1-3mg/ml) in the solution, soaked 5-10 minute, used the deionized water wash of pH=7.0 then earlier this thin film to be immersed PEI.Again BMP-2 and PDGF are processed the solution of 0.1-10 μ g/ml concentration, place thin film this solution to soak and adsorbed in 10-30 minute, and used the deionized water wash of pH=7.0 then.Immerse successively then PAH (PAH, 3mg/ml) and polyacrylic acid (PAA, 3mg/ml) in the solution, circulation 3-4 time is all used the deionized water eluting after each deposition.Next, P38 inhibitor (SB203580) and ERK inhibitor (PD98059) are dissolved in the solution that forms 0.1-15 μ g/ml concentration in the dimethyl sulfoxine (DMSO).Place this solution to soak 15-20 minute on the treated diaphragm in front.Use stream of nitrogen gas dry subsequently with ethanol elution then.Promptly obtain the degradable diaphragm for promoting regeneration of periodontal tissue.
As can be seen from Figure 1, PCL has formed nanofibrous structures through electrostatic spinning process, the PCL fibre diameter from several micron to the hundreds of nanometer.And fibrous membrane structure has porosity characteristic, can the useful space be provided for the growth factor loads.
Embodiment 2
Employing PLGA is a raw material, is dissolved in the chloroform, processes the solution that concentration is 20% (wt).This mixture solution is joined in the electrostatic spinning syringe through syringe pump control; The voltage of setting high tension generator is 20KV; Collecting distance is 20cm; Can obtain by the random thin film of forming of the superfine fibre of nanoscale diameter, then that this thin film is repeatedly for use after the final vacuum drying with deionized water wash.(PEI 1-3mg/ml) in the solution, soaked 5-10 minute, used the deionized water wash of pH=7.0 then earlier this thin film to be immersed PEI.Again BMP-2 and PDGF are processed the solution of 0.1-10 μ g/ml concentration, place thin film this solution to soak and adsorbed in 10-30 minute, and used the deionized water wash of pH=7.0 then.Immerse successively then PAH (PAH, 3mg/ml) and polyacrylic acid (PAA, 3mg/ml) in the solution, circulation 3-4 time is all used the deionized water eluting after each deposition.Next, P38 inhibitor (SB203580) and ERK inhibitor (PD98059) are dissolved in the solution that forms 0.1-15 μ g/ml concentration in the dimethyl sulfoxine (DMSO).Place this solution to soak 15-20 minute on the treated diaphragm in front.Use stream of nitrogen gas dry subsequently with ethanol elution then.Promptly obtain the degradable diaphragm for promoting regeneration of periodontal tissue.
As can be seen from Figure 2, PLGA has formed ultra-fine fiber construction through electrostatic spinning process, and the PLGA fibre diameter mainly is distributed in micron level.The PLGA superfine fibre is formed porous, random membrane structure.
Embodiment 3
Adopting PLGA and collagen protein (collagen) is raw material (2: 1), is dissolved in the hexafluoro propanol, processes the solution that concentration is 10% (wt).This mixture solution is joined in the electrostatic spinning syringe through syringe pump control; The voltage of setting high tension generator is 15KV; Collecting distance is 15cm; Can obtain by the random thin film of forming of the superfine fibre of nanoscale diameter, then that this thin film is repeatedly for use after the final vacuum drying with deionized water wash.BMP-2 and PDGF are processed the solution of 0.1-10 μ g/ml concentration, place thin film this solution to soak and adsorbed in 10-30 minute, and used the deionized water wash of pH=7.0 then.Immerse successively then PAH (PAH, 3mg/ml) and polyacrylic acid (PAA, 3mg/ml) in the solution, circulation 3-4 time is all used the deionized water eluting after each deposition.Next, P38 inhibitor (SB203580) and ERK inhibitor (PD98059) are dissolved in the solution that forms 0.1-15 μ g/ml concentration in the dimethyl sulfoxine (DMSO).Place this solution to soak 15-20 minute on the treated diaphragm in front.Use stream of nitrogen gas dry subsequently with ethanol elution then.Promptly obtain the degradable diaphragm for promoting regeneration of periodontal tissue.
As can be seen from Figure 3, PLGA/ collagen blending liquid has formed micron-sized superfine fibre through electrostatic spinning process, and has porous, random membrane structure, can be somatomedin load and cell adhesion advantage is provided.
Embodiment 4
Adopting PCL and modification of chitosan (chitosan) is raw material (1: 1), is dissolved in the hexafluoro propanol, processes the solution that concentration is 15% (wt).This mixture solution is joined in the electrostatic spinning syringe through syringe pump control; The voltage of setting high tension generator is 15KV; Collecting distance is 10cm; Can obtain by the random thin film of forming of the superfine fibre of nanoscale diameter, then that this thin film is repeatedly for use after the final vacuum drying with deionized water wash.BMP-2 and PDGF are processed the solution of 0.1-10 μ g/ml concentration, place thin film this solution to soak and adsorbed in 10-30 minute, and used the deionized water wash of pH=7.0 then.Immerse successively then PAH (PAH, 3mg/ml) and polyacrylic acid (PAA, 3mg/ml) in the solution, circulation 3-4 time is all used the deionized water eluting after each deposition.Next, P38 inhibitor (SB203580) and ERK inhibitor (PD98059) are dissolved in the solution that forms 0.1-15 μ g/ml concentration in the dimethyl sulfoxine (DMSO).Place this solution to soak 15-20 minute on the treated diaphragm in front.Use stream of nitrogen gas dry subsequently with ethanol elution then.Promptly obtain the degradable diaphragm for promoting regeneration of periodontal tissue.
Claims (3)
1. a biodegradable is urged the diaphragm-operated method for preparing of periodontal tissue's reparative regeneration; It is characterized in that in polylactic acid, polycaprolactone, polylactic acid-glycolic guanidine-acetic acid copolymer, collagen or the modification of chitosan one or more; Be dissolved in polarity, the volatile organic solvent; The concentration of control solution is 8-25wt%, through electrospinning process, makes the Biodegradable material thin film with nanostructured; Gained Biodegradable material thin film is adsorbed through the self assembly of supermolecule static; Will be to ERK cell pathway or the inhibited somatomedin and the somatomedin that promotes the periodontal osteanagenesis of P38 cell pathway; Be loaded to respectively on the Biodegradable material thin film; Through vacuum drying, behind the sterilization packaging, promptly get required product; Wherein: electrospinning process applies the high direct voltage scope and is: 0.5-50KV; Spinning head apart from the distance of gathering-device is: 5-100cm; Said inhibited somatomedin adopts SB203580 or PD98059, promotes that the somatomedin of periodontal osteanagenesis is BMP or PDGF.
2. biodegradable according to claim 1 is urged the diaphragm-operated method for preparing of periodontal tissue's reparative regeneration, it is characterized in that the polarity easy volatile solvent that is adopted is: the mixture of one or more solvents in chloroform, dichloromethane, methanol, ethanol, propanol, hexafluoro propanol, ethyl acetate or the acetone.
3. biodegradable according to claim 1 is urged the diaphragm-operated method for preparing of periodontal tissue's reparative regeneration, it is characterized in that said supermolecule static self assembly absorption is meant static self-assembling method layer by layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101055732A CN101791431B (en) | 2010-02-04 | 2010-02-04 | Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101055732A CN101791431B (en) | 2010-02-04 | 2010-02-04 | Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101791431A CN101791431A (en) | 2010-08-04 |
| CN101791431B true CN101791431B (en) | 2012-11-07 |
Family
ID=42584483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101055732A Expired - Fee Related CN101791431B (en) | 2010-02-04 | 2010-02-04 | Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101791431B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106492275A (en) * | 2016-11-11 | 2017-03-15 | 上海市口腔病防治院 | A kind of medicine/factor controlled release membranes support and preparation method thereof |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102926129A (en) * | 2012-11-09 | 2013-02-13 | 无锡中科光远生物材料有限公司 | Preparation method of electrostatic spinning macromolecule anti-adhesion fiber membrane with good flexibility |
| CN103349797B (en) * | 2013-07-22 | 2015-04-01 | 广西医科大学 | Absorbable polylactic acid film for enhancing airtightness of absorbable root canal post of deciduous tooth |
| CN103751847A (en) * | 2013-11-25 | 2014-04-30 | 同济大学 | Preparation method of tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating |
| CN104831476A (en) * | 2015-04-17 | 2015-08-12 | 东南大学苏州研究院 | Preparation method for degradable polylactic acid-glycolic acid/polycaprolactone/I-type collagen electrospun fiber membrane material |
| CN106310366B (en) * | 2016-09-29 | 2019-04-30 | 武汉生物工程学院 | A kind of Guide Periodontal Tissue Regeneration barrier film and the preparation method and application thereof |
| CN106521646B (en) * | 2016-10-05 | 2019-06-07 | 桂林理工大学 | A kind of preparation method of poly lactide-glycolide acid electrostatic spinning solution |
| CN106693070B (en) * | 2016-11-11 | 2020-09-08 | 上海市口腔病防治院 | Membranous biological repair material for periodontal tissue regeneration |
| CN106390193B (en) * | 2016-12-01 | 2019-04-23 | 上海市同济医院 | A kind of Biodegradable nano tunica fibrosa piece preparation method applied to the indirect vascular bypass art of neurosurgery |
| CN110064074A (en) * | 2018-01-23 | 2019-07-30 | 苏州博创同康生物工程有限公司 | A kind of compound support frame material and its preparation method and application |
| CN108096634B (en) * | 2018-03-07 | 2018-11-30 | 中国人民解放军陆军军医大学第一附属医院 | A kind of artificial bone graft's material and application thereof |
| CN110141687B (en) * | 2019-05-30 | 2021-10-08 | 四川大学 | Gradient material for guiding regeneration of periodontal hard and soft tissues and preparation method thereof |
| CN110559479B (en) * | 2019-09-12 | 2021-12-07 | 苏州高哲斯环境新材料有限公司 | Preparation method of tissue regeneration guiding membrane for tooth restoration |
| CN113209384B (en) * | 2021-05-08 | 2022-03-25 | 宁波市第一医院 | Pelvic floor patch for gynecology and preparation method thereof |
| CN114276295B (en) * | 2021-12-27 | 2023-12-05 | 南京医科大学附属口腔医院 | Guided tissue regeneration membrane containing TPE-NIM fluorescent probe and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739491A (en) * | 2005-09-15 | 2006-03-01 | 同济大学 | A kind of nanometer fiber slow-releasing system and its production and application |
| CN101280467A (en) * | 2008-05-20 | 2008-10-08 | 暨南大学 | Preparation and application of chitosan-based nano-fiber |
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
-
2010
- 2010-02-04 CN CN2010101055732A patent/CN101791431B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739491A (en) * | 2005-09-15 | 2006-03-01 | 同济大学 | A kind of nanometer fiber slow-releasing system and its production and application |
| CN101280467A (en) * | 2008-05-20 | 2008-10-08 | 暨南大学 | Preparation and application of chitosan-based nano-fiber |
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106492275A (en) * | 2016-11-11 | 2017-03-15 | 上海市口腔病防治院 | A kind of medicine/factor controlled release membranes support and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101791431A (en) | 2010-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101791431B (en) | Preparation method of biodegradable diaphragm for promoting regeneration of periodontal tissue | |
| Gao et al. | Electrospun nanofibers promote wound healing: Theories, techniques, and perspectives | |
| He et al. | Scaffold strategies for modulating immune microenvironment during bone regeneration | |
| Hasani-Sadrabadi et al. | Hierarchically patterned polydopamine-containing membranes for periodontal tissue engineering | |
| Kharbikar et al. | Modulating the foreign body response of implants for diabetes treatment | |
| Venugopal et al. | Interaction of cells and nanofiber scaffolds in tissue engineering | |
| Wang et al. | Collagen-based biomaterials for tissue engineering | |
| Eldeeb et al. | Biomaterials for tissue engineering applications and current updates in the field: A comprehensive review | |
| US20110038921A1 (en) | Methods and compositions for temporal release of agents from a biodegradable scaffold | |
| Kerimoglu et al. | Poly (lactic-co-glycolic acid) based drug delivery devices for tissue engineering and regenerative medicine | |
| Ping et al. | Modulating immune microenvironment during bone repair using biomaterials: focusing on the role of macrophages | |
| CN102497889A (en) | A method for producing a device applicable to biological tissues, particularly a patch for treating damaged tissues, and a device obtained by said method | |
| Wang et al. | Cell–scaffold interactions in tissue engineering for oral and craniofacial reconstruction | |
| Guo et al. | In vitro evaluation of electrospun PLGA/PLLA/PDLLA blend fibers loaded with naringin for guided bone regeneration | |
| CN103751847A (en) | Preparation method of tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating | |
| CN102423506A (en) | Medicine sustained release nanofiber membrane for treating periodontitis and preparation method for medicine sustained release nanofiber membrane | |
| Bu et al. | In situ precipitation of cluster and acicular hydroxyapatite onto porous poly (γ-benzyl-l-glutamate) microcarriers for bone tissue engineering | |
| CN103877621B (en) | Electrospun fiber-reinforced calcium phosphate bone cement composite material and application thereof | |
| Shabani et al. | Ion-exchange polymer nanofibers for enhanced osteogenic differentiation of stem cells and ectopic bone formation | |
| Zhu et al. | Advanced injectable hydrogels for cartilage tissue engineering | |
| EP3759265A1 (en) | Elongate scaffold comprising inner and outer portion | |
| Awasthi et al. | Recent progress in development of dressings used for diabetic wounds with special emphasis on scaffolds | |
| Wang et al. | Strategies of cell and cell-free therapies for periodontal regeneration: the state of the art | |
| Huang et al. | Controlled delivery of growth factor by hierarchical nanostructured core–shell nanofibers for the efficient repair of critical-sized rat calvarial defect | |
| KR101238539B1 (en) | A method of preparing nanofiber for bone tissue regeneration using biopolymer containing gelatin-apatite |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121107 Termination date: 20180204 |