CN101779967A - Method for directly measuring blood flowing velocity by using ultrasound microbubble (microsphere) contrast agent - Google Patents
Method for directly measuring blood flowing velocity by using ultrasound microbubble (microsphere) contrast agent Download PDFInfo
- Publication number
- CN101779967A CN101779967A CN200910076811A CN200910076811A CN101779967A CN 101779967 A CN101779967 A CN 101779967A CN 200910076811 A CN200910076811 A CN 200910076811A CN 200910076811 A CN200910076811 A CN 200910076811A CN 101779967 A CN101779967 A CN 101779967A
- Authority
- CN
- China
- Prior art keywords
- microbubble
- flowing velocity
- blood flow
- flow rate
- ultrasound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses a method for directly measuring blood flowing velocity by using ultrasound microbubble (microsphere) contrast agent and is characterized in that the method comprises the following steps that: 1) the ultrasound microbubble contrast agent is injected intravenously; 2) an ultrasonic contrast imaging technique is adopted to observe the movement of a microbubble signal and the movement is recorded; 3) the coordinates of microbubble in the image of each frame are positioned and the distance between the coordinates is calculated to obtain the moving distance of the microbubble; and 4) the calculated displacement of the microbubble signal is divided by time to obtain the flowing velocity of the microbubble. By adopting the method for measuring the blood flowing velocity, the invention has the advantages that the blood flowing velocity is directly through the ultrasound microbubble for the first time in the world, the flowing velocity of blood in capillaries can be particularly measured and the flowing velocity of blood in great vessels such as heart great vessels can be measured.
Description
Technical field
The present invention relates to a kind of method of measuring blood flow rate, be specifically related to a kind of method of utilizing ultrasound microbubble contrast agent commercial measurement blood flow rate to comprise blood flow rate in the trunks such as little blood flow and heart.
Background technology
The blood flow measurement technology is started late, adopt indicator dilution method the earliest, has occurred the indicator tracing subsequently and based on the electromagnetic blood flowmeter of Hall effect etc., but the shortcoming of these method maximums has wound to tissue exactly.In order to reach non-invasive measurement, two kinds of gauges based on Doppler effect have appearred in succession---laser-Doppler blood flowmeter and ultrasonic doppler blood flow amount meter.Wherein laser method only can be measured the blood flow rate of very shallow table, almost can not be used for clinical blood flow velocity measurement.The multispectral method of reining in is by emission pulse ultrasonic (or continuous ultrasound ripple, irradiation target (blood vessel), and receive its scatter echo signal, by demodulation, obtain doppler frequency (frequency displacement) fd of echo, can obtain corresponding blood flow velocity, but influenced by angle, signal intensity etc., blood flow rate can not be accurately measured in some cases, and the measurement of little blood flow rate can't be carried out.
Also having a kind of method is the MRA method.It belongs to one of MR imaging method, utilizes the technology of the flowing effect demonstration blood vessel image of blood.Different with the orthovoltage x-ray angiography, MRA does not need the inject contrast material usually.The technology that is mainly used in MRA leap if having time method (time of flight, TOF), the phase-contrast method (phase contrast, PC) and black blood technology.With another difference of orthovoltage x-ray angiography be that the signal of MRA can two dimension or three-dimensional acquisition and demonstration, also can two dimension combine collection (MOTSA) with three dimensional constitution.So far, MRA is mainly used in the imaging than trunk, can't replace routine angiography or DSA in the demonstration of capillary vessel, and it costs an arm and a leg.
As seen by aforementioned, prior art has the shortcoming that himself is difficult to overcome.Therefore, the method for seeking a kind of new measurement blood flow rate also just becomes the industry common recognition.
In recent years, emerging ultrasonic contrast technology is along with the continuous development of acoustic contrast and technology of preparing reaches its maturity and the rapid raising of ultrasonic instrument resolution, and people such as professor Klibanov find: one microvesicle also can be by ultrasonic discovery.Concrete document is referring to The measurement of backscatter fromindividual contrast agent microbubblesSboros, V.; Pye, S.D.; Anderson, T.; Moran, C.M.; Averkiou, M.; MacDonald, C.A.; Gomatam, J.Ultrasonics Symposium, 2002.Proceedings.2002IEEEVolume 2, Issue, 8-11Oct.2002Page (s): 1945-1947vol.2Digital Object Identifier 10.1109/ULTSYM.2002.1192680
Find that based on this we propose to utilize ultrasonic microbubble directly to measure the theory of blood flow rate first, comprise the blood flow rate in little blood flow rate and the cardiovascular injuries in the world.
Summary of the invention
It is the method that the measurement blood flow rate of medium comprises blood flow rate in the trunks such as blood capillary and heart with ultrasonic microbubble (microsphere) that the technical problem to be solved in the present invention provides a kind of.
In order to solve the problems of the technologies described above, a kind of using ultrasound microbubble of the present invention (microsphere) is directly measured the method for blood flow rate, and it is characterized in that: it comprises following steps:
1) intravenous injection ultrasound microbubble contrast agent;
2) adopt moving of ultrasonic contrast imaging technique observation microbubble signals, and video recording;
3) coordinate of microvesicle in the every loyal image in location, the distance between coordinates computed and obtain the distance that microvesicle moves then;
4) obtain the flowing velocity of microvesicle divided by the time by the displacement of calculating microbubble signals.
Further, step 2) described ultrasonic contrast imaging technique comprises and all can show the technology that microbubble signals moves, as harmonic wave imaging technique, subharmonic technology etc.
Further again, step 3) is with the motion track of software track microbubble signals, comprises manual tracking and automatic tracking method.
Adopt the method for the measurement blood flow rate of technique scheme, its beneficial effect is to utilize ultrasonic microbubble directly to measure blood flow rate in the world first, especially can directly measure the blood flow rate in the blood capillary, certainly, also can measure the blood flow rate in the trunks such as comprising heart.
Description of drawings
Below in conjunction with drawings and Examples technical solution of the present invention is further specified:
Fig. 1 is that one of embodiment of the invention is obtained the sketch map that the ultrasonic microbubble signal moves picture recording to experiment in vitro.
Fig. 2 is the sketch map that one of embodiment of the invention is obtained the coordinate of microbubble signals in every loyal image.
Fig. 3 is that one of embodiment of the invention is calculated the blood flow rate of gained and the standard speed of pump compares, and difference does not have the sketch map of significance (p＞0.05).
Fig. 4 is that two of the embodiment of the invention shows the mobile sketch map of microbubble signals in the tumor blood capillary.
Fig. 5 is that two of the embodiment of the invention utilizes DFY quantitative analysis diagnostic apparatus to draw the motion track of microbubble signals in the tumor blood capillary and the sketch map of computational speed.
The specific embodiment
Fig. 1-Fig. 3 has provided first embodiment of the present invention.
The microvesicle that present embodiment uses adopts the Powdered phospholipid microvesicle of spray affair seasoning preparation, and its Main Ingredients and Appearance is phosphatidylcholine, PHOSPHATIDYL ETHANOLAMINE, perfluoropropane gas.The microvesicle diameter is between the 1-3 micron.Specific practice can be referring to another related application of the inventor, title: emulsion spray drying method for preparing ultrasound microbubble contrast agent in batch; Application number is 200610103942.8; Open (bulletin) number: CN1958076
Certainly, the ultrasound microbubble contrast agent that the present invention can also adopt other method to obtain, employed instrument and equipment and material etc. all are can be by the conventional equipment and the material of commercial channel acquisition.
In Fig. 1, the preparation external model adopts the harmonic wave imaging technique of colorful ultrasonic aplio to observe moving of microbubble signals and picture recording.
In this technical field, using maximum at present is the harmonic wave imaging technique, and the subharmonic imaging technique uses lessly relatively.If but can show microbubble signals, can use in the present invention.
In Fig. 2, measure the coordinate of microbubble signals in every loyal image.
In Fig. 3, draw the motion track of microvesicle and calculate its speed with DFY quantitative analysis diagnostic apparatus.
What present embodiment adopted is ultrasonic quantitative analysis diagnostic apparatus (the production permit lot number of DDFY common on the market; River medicine device is supervised accurate word 97 No. 223001).Can certainly select the product of other model and specification for use.
Use the ultrasonic quantitative analysis diagnostic apparatus of DFY by the loyal coordinate that obtains the microbubble signals central point, calculate the distance between every loyal coordinate automatically, promptly obtain instantaneous translational speed divided by the time that every chastity experienced with this distance; With after the coordinate distance addition between all chastity again divided by the average speed that promptly obtains the total time that all chastity experienced moving;
Automatic tracking method is meant by the radiofrequency signal of microvesicle being analyzed or the image file that obtains behind the ultrasonic instrument processing initial data being carried out computer handle automatically, distinguish single microbubble signals, and calculate the coordinate of microbubble signals central point automatically, adopt formula to calculate instantaneous velocity and the average speed that moves then with manually method is identical.
In the present embodiment, the flow rate of liquid of 3 groups of standards is respectively 37.14MM/S among Fig. 3,21.01MM/S, 4.35MM/S, be 36.55MM/S ± 3.5MM/S with the speed that this law obtained, 25.04MM/S ± 2.8MM/S, 4.80MM/S ± 1.0MM/S, there was no significant difference between corresponding group;
Fig. 4-Fig. 5 has provided second embodiment of the present invention.
At first, preparation subcutaneous rat tumor model adopts the harmonic wave imaging technique of colorful ultrasonic aplio to observe moving of microbubble signals and picture recording.
Secondly, measure the coordinate of microbubble signals in every loyal image, as shown in Figure 4.
At last, draw the motion track of microvesicle and calculate its speed with DFY quantitative analysis diagnostic apparatus, as shown in Figure 5.
Claims (3)
1. a using ultrasound microbubble (microsphere) is directly measured the method for blood flow rate, and it is characterized in that: it comprises following steps:
1) intravenous injection ultrasound microbubble contrast agent;
2) adopt moving of ultrasonic contrast imaging technique observation microbubble signals, and video recording;
3) coordinate of microvesicle in the every loyal image in location, the distance between coordinates computed and obtain the distance that microvesicle moves then;
4) obtain the flowing velocity of microvesicle divided by the time by the displacement of calculating microbubble signals.
2. using ultrasound microbubble as claimed in claim 1 (microsphere) is directly measured the method for blood flow rate, it is characterized in that: step 2) described in the ultrasonic contrast imaging technique comprise and all can show the technology that microbubble signals moves, as harmonic wave imaging technique, subharmonic technology etc.
3. measure the method for blood flow rate as claim 1 ultrasonic microbubble, it is characterized in that: step 3) is with the motion track of software track microbubble signals, comprises manual tracking and automatic tracking method, the automatic tracking technique of lay special stress on microbubble signals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910076811A CN101779967A (en) | 2009-01-21 | 2009-01-21 | Method for directly measuring blood flowing velocity by using ultrasound microbubble (microsphere) contrast agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910076811A CN101779967A (en) | 2009-01-21 | 2009-01-21 | Method for directly measuring blood flowing velocity by using ultrasound microbubble (microsphere) contrast agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101779967A true CN101779967A (en) | 2010-07-21 |
Family
ID=42520206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910076811A Pending CN101779967A (en) | 2009-01-21 | 2009-01-21 | Method for directly measuring blood flowing velocity by using ultrasound microbubble (microsphere) contrast agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101779967A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340620A (en) * | 2013-05-31 | 2013-10-09 | 中国科学院深圳先进技术研究院 | Tube wall stress phase angle measuring method and system |
| CN103839281A (en) * | 2014-03-27 | 2014-06-04 | 武汉大学 | Method for measuring blood flow velocity by utilization of radiography microbubbles |
| CN107753062A (en) * | 2017-11-27 | 2018-03-06 | 西安交通大学 | TCD,transcranial Doppler cerebral angiography super-resolution imaging method based on Markov chain Monte-Carlo multi-target tracking |
| CN109152563A (en) * | 2016-03-22 | 2019-01-04 | 帝国创新技术有限公司 | Fluid stream analysis |
| CN110279393A (en) * | 2018-03-19 | 2019-09-27 | 洋华光电股份有限公司 | Capilary detection device and method |
| CN111728643A (en) * | 2020-06-24 | 2020-10-02 | 南京超维景生物科技有限公司 | Single blood vessel ultrasonic blood flow perfusion imaging method and device thereof |
| CN112603372A (en) * | 2020-12-22 | 2021-04-06 | 安徽医科大学第一附属医院 | Method for obtaining blood flow in ultrasonic image |
| CN112785580A (en) * | 2021-01-28 | 2021-05-11 | 深圳睿心智能医疗科技有限公司 | Method and device for determining blood vessel flow velocity |
-
2009
- 2009-01-21 CN CN200910076811A patent/CN101779967A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340620B (en) * | 2013-05-31 | 2016-03-30 | 中国科学院深圳先进技术研究院 | A kind of measuring method of tube wall stress phase angle and system |
| CN103340620A (en) * | 2013-05-31 | 2013-10-09 | 中国科学院深圳先进技术研究院 | Tube wall stress phase angle measuring method and system |
| CN103839281A (en) * | 2014-03-27 | 2014-06-04 | 武汉大学 | Method for measuring blood flow velocity by utilization of radiography microbubbles |
| US11298103B2 (en) | 2016-03-22 | 2022-04-12 | Imperial College Innovations Limited | Fluid flow analysis |
| CN109152563A (en) * | 2016-03-22 | 2019-01-04 | 帝国创新技术有限公司 | Fluid stream analysis |
| CN107753062A (en) * | 2017-11-27 | 2018-03-06 | 西安交通大学 | TCD,transcranial Doppler cerebral angiography super-resolution imaging method based on Markov chain Monte-Carlo multi-target tracking |
| CN107753062B (en) * | 2017-11-27 | 2021-03-16 | 西安交通大学 | Transcranial ultrasonic cerebrovascular angiography super-resolution imaging method based on Markov chain Monte Carlo multi-target tracking |
| CN110279393A (en) * | 2018-03-19 | 2019-09-27 | 洋华光电股份有限公司 | Capilary detection device and method |
| CN110279393B (en) * | 2018-03-19 | 2023-04-28 | 洋华光电股份有限公司 | Microvascular detection device and method |
| CN111728643A (en) * | 2020-06-24 | 2020-10-02 | 南京超维景生物科技有限公司 | Single blood vessel ultrasonic blood flow perfusion imaging method and device thereof |
| CN112603372A (en) * | 2020-12-22 | 2021-04-06 | 安徽医科大学第一附属医院 | Method for obtaining blood flow in ultrasonic image |
| CN112785580A (en) * | 2021-01-28 | 2021-05-11 | 深圳睿心智能医疗科技有限公司 | Method and device for determining blood vessel flow velocity |
| CN112785580B (en) * | 2021-01-28 | 2024-02-02 | 深圳睿心智能医疗科技有限公司 | Method and device for determining vascular flow velocity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101779967A (en) | Method for directly measuring blood flowing velocity by using ultrasound microbubble (microsphere) contrast agent | |
| Heiles et al. | Ultrafast 3D ultrasound localization microscopy using a 32$\times $32 matrix array | |
| Leow et al. | Flow velocity mapping using contrast enhanced high-frame-rate plane wave ultrasound and image tracking: Methods and initial in vitro and in vivo evaluation | |
| Ackermann et al. | Detection and tracking of multiple microbubbles in ultrasound B-mode images | |
| Gessner et al. | Mapping microvasculature with acoustic angiography yields quantifiable differences between healthy and tumor-bearing tissue volumes in a rodent model | |
| Kim et al. | Noninvasive measurement of steady and pulsating velocity profiles and shear rates in arteries using echo PIV: in vitro validation studies | |
| Lucidarme et al. | Blood flow quantification with contrast-enhanced US:“entrance in the section” phenomenon—phantom and rabbit study | |
| Guo et al. | Three-dimensional power Doppler imaging: a phantom study to quantify vessel stenosis | |
| Prinz et al. | Can echocardiographic particle image velocimetry correctly detect motion patterns as they occur in blood inside heart chambers? A validation study using moving phantoms | |
| Calderon-Arnulphi et al. | In vivo evaluation of quantitative MR angiography in a canine carotid artery stenosis model | |
| Yeom et al. | Improvement of ultrasound speckle image velocimetry using image enhancement techniques | |
| Hansen et al. | Vector flow imaging compared with digital subtraction angiography for stenosis assessment in the superficial femoral artery–a study of vector concentration, velocity ratio and stenosis degree percentage | |
| Li et al. | Flow convergence flow rates from 3-dimensional reconstruction of color Doppler flow maps for computing transvalvular regurgitant flows without geometric assumptions: an in vitro quantitative flow study | |
| CN101647716B (en) | Device for directly measuring blood flow velocity | |
| HISTAND et al. | Transcutaneous measurement of blood velocity profiles and flow | |
| Tsujino et al. | Quantitative measurement of volume flow rate (cardiac output) by the multibeam Doppler method | |
| Riemer et al. | Contrast agent-free assessment of blood flow and wall shear stress in the rabbit aorta using ultrasound image velocimetry | |
| Niu et al. | Real-time texture analysis for identifying optimum microbubble concentration in 2-D ultrasonic particle image velocimetry | |
| Niu et al. | A 2 D non‐invasive ultrasonic method for simultaneous measurement of arterial strain and flow pattern | |
| CN103340620B (en) | A kind of measuring method of tube wall stress phase angle and system | |
| Kenwright et al. | A protocol for improved measurement of arterial flow rate in preclinical ultrasound | |
| Gaitini | Introduction to color Doppler ultrasound of the skin | |
| CN104546012B (en) | Cardiac function assessment method and apparatus | |
| Picot et al. | Rapid volume flow rate estimation using transverse colour Doppler imaging | |
| Claudon et al. | Power Doppler US: evaluation of the morphology of stenoses with a flow phantom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20100721 |