CN101768644B

CN101768644B - Method utilizing enzyme to catalyze H2O2 to discolor sugar juice

Info

Publication number: CN101768644B
Application number: CN2010100456406A
Authority: CN
Inventors: 陆海勤; 陈永生; 谢彩锋; 李凯; 李淑贞; 杨同香
Original assignee: Guangxi University
Current assignee: Guangxi University
Priority date: 2010-01-20
Filing date: 2010-01-20
Publication date: 2012-03-28
Anticipated expiration: 2030-01-20
Also published as: CN101768644A

Abstract

The invention discloses a method utilizing enzyme to catalyze H2O2 to discolor sugar juice. The method includes the following steps: in the clearing technique of the sugarcane or beet sugar manufacturing process, peroxidase and the H2O2 are adopted to discolor the sugar juice; the peroxidase and the H2O2 are diluted by softened water, the diluted concentration of the peroxidase is within 0.01 to 0.1%, and the diluted concentration of the H2O2 is within 0.01 to 0.05%; the peroxidase and the H2O2 are mixed together according to the proportion of 1:8 to 8:1 so as to obtain peroxidase mixed solution; finally, the peroxidase mixed solution is added to the sugar juice to discolor the sugar juice. The method in the invention can effectively remove polyphenols in the sugar juice, lower the color value, improve the product quality of white granulated sugar, reduce the residual sulfur content, and advance the product grade.

Description

A kind of enzyme catalysis H that uses 2O 2The method that syrup is decoloured

Technical field

The present invention relates to reduce in the sugar manufacturing process method of syrup colour, specifically is a kind of enzyme catalysis H that uses ₂O ₂The method that syrup is decoloured.

Technical background

The pigment that sugarcane itself contains has fat-soluble and water-soluble two big types; Fat-soluble pigment is water insoluble basically, and major part can be removed when adding the ash heating, does not influence quality product; Water colo(u)r mainly is various polyphenols, and they are the major causes that make white sugar and various sugar products band looks.Research shows, in sugar refining technology defecation method at present, the sulfurous method clarification can be removed about 30% of aldehydes matter in the mixing juice, and the carbonatation process clarification can be removed about 50%.The polyphenols that the end is removed gets into crystal in sugar boiling process, influence quality product.If the aldehydes matter that contains in the crystal is more than a certain numerical value, variable color easily in storage process influences the grade of product.

In clarifying process, more removing polyphenols, is one of measure that guarantees quality product.The efficient specificity of enzyme has obtained widespread use in food service industry, and px is the plant extract enzyme, is a kind of safe foodstuff additive.

Summary of the invention

The purpose of this invention is to provide a kind of with superoxide enzyme catalysis H ₂O ₂Polyphenol oxidation in the syrup is formed deposition remove, make the method for sugar juice decoloring.

The principle of decolouring is following:

But px catalysis H ₂O ₂The product of oxidation phenolic material is a quinones, and the further condensation of quinones forms HMW and water-insoluble polymer, and its primitive reaction is:

AH+H ₂O ₂→·A+H ₂O

In the formula: AH is a substrate phenol, and A is a radical, and the high reactivity benzene oxyradical of generation etc. is diffused into solution everywhere, itself reacts with phenol molecule or benzene oxyradical, generates the polymkeric substance of a series of poorly water-solubles, can remove through deposition or filtration.

The technical scheme that the present invention solves the problems of the technologies described above is:

A kind of enzyme catalysis H that uses ₂O ₂To the method that syrup is decoloured, this method is in the clarification process of sugarcane or beet sugar manufacture process, with px and H ₂O ₂With the softening water dilution, the px weaker concn is 0.01%～0.1%, H ₂O ₂Weaker concn is 0.01～0.05%, with 1: 8～8: 1 mixed and after becoming the px mixed solution, adds in the syrup, and syrup is decoloured.

Above-mentioned syrup is the mixing juice that cane milling workshop section comes out, and with the px mixed solution syrup is decoloured, and the addition of px mixed solution is the 0.1ppm～10ppm of mixing juice weight.

Above-mentioned syrup is to ooze out the diffusion juice that workshop section comes out from beet or sugarcane, with the px mixed solution diffusion juice is decoloured, and the addition of px mixed solution is the 0.1ppm～10ppm of diffusion juice weight.

Above-mentioned syrup is the clear juice that comes out from clarification workshop section, with the px mixed solution clear juice is decoloured, and the addition of px mixed solution is the 0.1ppm～10ppm of clear juice weight.

Above-mentioned syrup is the raw sugar slurry or the retro-dissolved syrup that come out from evaporation section, with the px mixed solution raw sugar slurry or retro-dissolved syrup is decoloured, and the addition of px mixed solution is the 0.1ppm～10ppm of syrup weight.

Above-mentioned syrup refers to any two kinds, three kinds or four kinds in mixing juice, diffusion juice, clear juice, syrup or the retro-dissolved syrup; Add the px mixed solution in the syrup to amount be the 0.1ppm～10ppm of all kinds of syrup weight, its total consumption is not more than the 30ppm of all kinds of syrup gross weights of being decoloured.

The present invention compared with prior art has following effect:

1. improve the decolorizing effect of clarifying process, in various syrups, use, the rate of removing of polyphenol reaches 20%～80%, and colour reduces about 50%, and effect is obvious, thereby can improve the quality of products;

2. reduce the white sugar residual sulfur content.The sulfurous method clarification process mainly is to remove through sulfurous acid deposition adsorpting pigment, uses the present invention can suitably reduce intensity of sulfitation, reduces the consumption of sulphur, and then reduces the residual of sulfurous gas in the white sugar, improves food safety.

3. technology is simple, need not to improve original technology, only needs to increase dilution tank and adds pipeline and valve, and consumption seldom; Cost is low, and efficient is high, can reduce the syrup colour significantly; Improve product hierarchy, and reduce the consumption of other finings (lime, sulphur, phosphoric acid), remarkable in economical benefits.

Embodiment

Below in conjunction with embodiment content of the present invention is further described.But need to prove that embodiment does not constitute the restriction to claim protection domain of the present invention.

Embodiment one

In the mixing juice of milling shop, add px and H ₂O ₂Mixture, px weaker concn are 0.05%, H ₂O ₂Weaker concn is 0.02%, and with 8: 1 mixed, its consumption contains the phenol amount according to mixing juice to be decided after the dilution; Add-on is 0.1ppm～10ppm; Need not increase whipping appts, in the pumping process, mix and reaction, the reaction times from be added to stove drying with before; Totally 20 minutes, the polyphenol rate of removing was 17.82%.

Embodiment two

Add px and H in the clear juice after the clarification of sugarcane sulfurous method ₂O ₂Mixture, px weaker concn are 0.05%, H ₂O ₂Weaker concn is 0.02%, and with 6: 2 mixed, its consumption was decided according to color value of clear juice after the dilution; Add-on is 0.1ppm～10ppm, need not increase whipping appts, in the pumping process, mixes and reaction; Reaction times finishes from being added to evaporative process; Totally 60 minutes, the polyphenol rate of removing was 59.53%, and colour reduces by 42.25%.

Embodiment three

In the raw sugar slurry that evaporation section comes out, add px and H ₂O ₂Mixture, px weaker concn be for O.01%, H ₂O ₂Weaker concn is for O.01%, after the dilution, with 1: 8 mixed; Its consumption is decided according to the syrup colour, add-on for O.1ppm～1Oppm, need not increase whipping appts; In the pumping process, mix and reaction, the reaction times from be added to get into boiling pan before, totally 30 minutes; The polyphenol rate of removing is 76.07%, and colour reduces by 51.42%.

As a kind of conversion of the embodiment of the invention, also can be used for the syrup of beet-sugar factory, and as required, with px and H ₂O ₂Add any two kinds, three kinds or four kinds in mixing juice, diffusion juice, clear juice, raw sugar slurry or the retro-dissolved syrup simultaneously respectively to; Consumption is decided according to the colour height of syrup; Add to px amount in the syrup be all kinds of syrup weight O.1ppm～10ppm, its total consumption is not more than the 30ppm of all kinds of syrup gross weights of being decoloured.

The embodiment of the invention is described px and H ₂O ₂Be required to be the solution after softening water dilutes, can directly add in the corresponding syrup.

Claims

1. use enzyme catalysis H for one kind ₂O ₂Method to syrup is decoloured is characterized in that: this method adopts px and H in the clarification process of sugarcane or beet sugar manufacture process ₂O ₂Syrup is decoloured, with px and H ₂O ₂With the softening water dilution, the px weaker concn is 0.01%～0.1%, H ₂O ₂Weaker concn is 0.01～0.05%, become the px mixed solution with 1: 8～8: 1 mixed after, add in the syrup, it is decoloured.

2. a kind of enzyme catalysis H that uses according to claim 1 ₂O ₂Method to syrup is decoloured is characterized in that: described syrup is the mixing juice that cane milling workshop section comes out, and with the px mixed solution syrup is decoloured, and the addition of px mixed solution is the 0.1ppm～10ppm of mixing juice weight.

3. a kind of enzyme catalysis H that uses according to claim 1 ₂O ₂The method that syrup is decoloured; It is characterized in that: described syrup is to ooze out the diffusion juice that workshop section comes out from beet or sugarcane; With the px mixed solution diffusion juice is decoloured, the addition of px mixed solution is the 0.1ppm～10ppm of diffusion juice weight.

4. a kind of enzyme catalysis H that uses according to claim 1 ₂O ₂Method to syrup is decoloured is characterized in that: described syrup is the clear juice that comes out from clarification workshop section, with the px mixed solution clear juice is decoloured, and the addition of px mixed solution is the 0.1ppm～10ppm of clear juice weight.

5. a kind of enzyme catalysis H that uses according to claim 1 ₂O ₂The method that syrup is decoloured; It is characterized in that: described syrup is the raw sugar slurry or the retro-dissolved syrup that come out from evaporation section; With the px mixed solution raw sugar slurry or retro-dissolved syrup are decoloured, the addition of px mixed solution is the 0.1ppm～10ppm of syrup weight.

6. a kind of enzyme catalysis H that uses according to claim 1 ₂O ₂The method that syrup is decoloured; It is characterized in that: described syrup refers to any two kinds, three kinds or four kinds in mixing juice, diffusion juice, clear juice, raw sugar slurry or the retro-dissolved syrup; Add the px mixed solution in the syrup to amount be the 0.1ppm～10ppm of all kinds of syrup weight, its total consumption is not more than the 30ppm of all kinds of syrup gross weights of being decoloured.