CN101766507A - Calf gender-determined reproduction method - Google Patents
Calf gender-determined reproduction method Download PDFInfo
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- CN101766507A CN101766507A CN200810148078A CN200810148078A CN101766507A CN 101766507 A CN101766507 A CN 101766507A CN 200810148078 A CN200810148078 A CN 200810148078A CN 200810148078 A CN200810148078 A CN 200810148078A CN 101766507 A CN101766507 A CN 101766507A
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Abstract
The invention discloses a calf gender-determined reproduction method which is used for the artificial reproduction of calves. The technical scheme is that the pH value of the vaginal mucus of a maternal body is determined before mating; when the pH value is larger than 7, the born calves after mating are male calves; and when the pH valve is less than 7, the born calves after mating are female calves. The invention has the advantages that the gender-determined reproduction can be accurately realized, the development of the cattle raising industry is effectively promoted, the progress of the breed improvement is promoted and the economic benefit of cattle raisers is increased; and the technical measures are simple and feasible, and the popularization and the application in rural areas are facilitated.
Description
Technical field
The present invention relates to a kind of qualitative mating system that calf breeds that is used for.
Background technology
Along with the development of cattle-raising, be to improve the economic benefit of cattle-raising, the dynamics to breed improvement has been strengthened in various places, yet because frozen semen breeding broad application, the phenomenon that ratio that cattle produces female calf descends occurred.Statistics shows that it only is 32.4~47% that the cattle of application frozen semen breeding produces female calf rate.The development that this can seriously restrict cattle-raising undoubtedly influences the process of various places breed improvement, has also influenced the raising of cowboying family economic benefit.
In recent years, the sex controll technology has progressively obtained application in breeding calf.Sex controll is meant by intervening artificially or operation, makes parent breed a kind of biotechnology of required sex offspring according to people's hope.At present, be used for the sex controll technology that calf breeds and mainly contain following two kinds, the one, embryo's biotechnology, as utilize individual size, the density of X, y sperm and institute is electrically charged with centrifuging, Filtration, electrophoresis method etc. the X sperm is separated with y sperm, obtain required sex sperm, perhaps before embryo transfer, embryo gender is identified, yet owing to be subjected to the restriction of factors such as cost, technical conditions, this method is not applied in production practices; The 2nd, utilize breeding insemination environmental condition control calf sex ratio, be representative with the arginine method.The arginine method is to be high, medium and low concentration with normal saline with arginine dilution, preceding 20~the 30min of semen deposition imports a certain concentration solution 1~2ml to the intravaginal of breeding parent, import high and low concentration and can improve male calf productive rate, then can improve female calf productive rate during the input intermediate concentration.Although it is about about 80% that the arginine method can be brought up to the productive rate of female calf, yet still have sizable gap from qualitative breeding.So-called qualitative breeding is meant and hundred-percently according to producing needs breeds out male calf or female calf.
Summary of the invention
Technical problem to be solved by this invention provides a kind of qualitative mating system that calf breeds that is used for.
The technical solution adopted for the present invention to solve the technical problems is: calf gender-determined reproduction method of the present invention is characterized in that: determine the mucous pH value of parent vagina before the breeding, when pH value greater than 7 the time, the calf that breeding is given birth to is male cattle calf; When pH value less than 7 the time, the calf that breeding is given birth to is female calf.
In technique scheme, when the parent age be can not determine, definite pH reagent paper that can adopt of described pH value was measured.
In technique scheme, when the parent age can be determined, the following calculating formula of definite employing of described pH value was calculated:
Parent below 10 years old: (9+ breeding month a umber)-parent age+9, the last digit of its total calf that is given birth to that breeds during for odd number is male calf, and the last digit of its total calf that is given birth to that breeds during for even number is female cattle calf;
Parent more than 10 years old: (19+ breeding month a umber)-parent age+19, the last digit of its total calf that is given birth to that breeds during for odd number is male calf, and the last digit of its total calf that is given birth to that breeds during for even number is female cattle calf;
In the formula: the parent age was as the criterion with the copulation reproductive age, and a breeding month umber is as the criterion with the lunar calendar.
The invention has the beneficial effects as follows, can realize accurately that the qualitative of cattle breeds, can promote the development of cattle-raising effectively, promote the process of breed improvement, improve cowboying family economic benefit; Technical measures are simple, help applying in vast rural area.
The specific embodiment
The present invention is further described below in conjunction with drawings and Examples.
Calf gender-determined reproduction method of the present invention is characterized in that, determines the mucous pH value of parent vagina before the breeding, when pH value greater than 7 the time, the calf that breeding is given birth to is male cattle calf; When pH value less than 7 the time, the calf that breeding is given birth to is female calf.
Scientist discovers the sex-determination factor, in the reproductive process of the single tire of mammal, after male subtracts plain the separation, formation contains two kinds of chromosomal spermatids of X, Y, female individuals subtracts the plain back of separating with regard to a kind of ovum cell that contains X chromosome, and in fertilization process, form the male that develops into of XY type zygote, form the female individuals that develops into of XX type.The key of sex difference is exactly the spermatid that male contains Y chromosome.
The applicant is by discovering that to what cattle and calf bred the control of sex is that female individuals determines for many years, and the sex-controlled key of cattle just is the selectivity of parent to X, two kinds of spermatids of Y.Parent is in fertilization process, and parent depends on the physiological law of parent self again to the selectivity of the spermatid that contains X, Y chromosome, and parent has maximum sex controll power.By to the sexes of 100 calves and breed the age of parent, the investigation and the analysis in breeding month, prove that the age of calf sex and parent, the month of breeding are closely related, there is corresponding relation in the while with the mucous pH value of parent vagina.Did experiment with the cow of oneself feeding as 1985, cow did not breed in 1 year, and 3 years old age is when each oestrus, mucus flowed out, inserting vagina with the pH reagent paper takes out and pH reagent paper chromatograph check analysis, with chromatograph value 7 is neutrality, subtract a little less than greater than 7 being, and be weak acid less than 7, this cow of 3 years old is in this year as a result, the lunar calendar 3,5,7,9, November, pH value is weak acid less than 7,2,4,6,8,10, the December pH value subtracts a little less than being greater than 7.1986, to the test that uses the same method of 6 years old cow, the result the lunar calendar 1,3,5,7,9, November, pH value subtracts a little less than being greater than 7,2,4,6,8,10, the December pH value is weak acid less than 7.1987~1998 years, respectively to 2,4,5,8,10,11,14,16 years old cow rotates test, under the situation of in a year, not breeding, test with the pH reagent paper, the result who measures is, the age of cow be odd number at the pH value of odd number month of lunar calendar test less than 7, the age is even, test month is that the pH value of odd number is greater than 7.
Therefore, when the age of breeding parent be can not determine, definite pH reagent paper that can adopt of described pH value was measured.When the age of breeding parent can be determined, the following calculating formula of definite employing of described pH value was calculated:
Parent below 10 years old: (9+ breeding month a umber)-parent age+9, the last digit of its total calf that is given birth to that breeds during for odd number is male calf, and the last digit of its total calf that is given birth to that breeds during for even number is female cattle calf;
Parent more than 10 years old: (19+ breeding month a umber)-parent age+19, the last digit of its total calf that is given birth to that breeds during for odd number is male calf, and the last digit of its total calf that is given birth to that breeds during for even number is female cattle calf;
In the formula: the parent age was as the criterion with the copulation reproductive age, and a breeding month umber is as the criterion with the lunar calendar.
The applicant is formal qualitative breeding breeding test in 1999, and 2 years old then cow is in lunar calendar breeding on February 23, and the pH test is less than 7 before the breeding, and qualitative is female, the female calf of lunar calendar December 28 daily outputs then.7 years old then cow lunar calendar breeding on March 20, the PH test is less than 7 before the breeding, and numerical calculation is an even number, and qualitative is female, gives birth to female calf at the positive beginning of the month 4 in 2000.2000 with pH value method of testing breeding, lunar calendar breeding on February 13, and pH tests less than 7, and qualitative is female, and giving birth to calf November 28 then is cow.When 3 years 13 years old cow lunar calendar breeding on March 13, pH tested less than 7, and qualitative is female, and twelfth month 29 was given birth to female calf then.
When adopting frozen semen to join essence, when the pH value of breeding parent vagina mucus and frozen semen all greater than 7 the time, the calf that breeding is given birth to is male cattle calf; When the pH value of breeding parent vagina mucus and frozen semen all less than 7 the time, the calf that breeding is given birth to is female calf.
Claims (4)
1. calf gender-determined reproduction method is characterized in that: determine the mucous pH value of parent vagina before the breeding, when pH value greater than 7 the time, the calf that breeding is given birth to is male cattle calf; When pH value less than 7 the time, the calf that breeding is given birth to is female calf.
2. calf gender-determined reproduction method according to claim 1 is characterized in that: when adopting frozen semen to join essence, when the pH value of breeding parent vagina mucus and frozen semen all greater than 7 the time, the calf that breeding is given birth to is male cattle calf; When the pH value of breeding parent vagina mucus and frozen semen all less than 7 the time, the calf that breeding is given birth to is female calf.
3. calf gender-determined reproduction method as claimed in claim 1 or 2, it is characterized in that: determining of described pH value is to adopt the pH reagent paper to measure.
4. calf gender-determined reproduction method as claimed in claim 1 or 2, it is characterized in that: the following calculating formula of definite employing of described pH value is calculated:
Parent below 10 years old: (9+ breeding month a umber)-parent age+9, the last digit of its total calf that is given birth to that breeds during for odd number is male calf, and the last digit of its total calf that is given birth to that breeds during for even number is female cattle calf;
Parent more than 10 years old: (19+ breeding month a umber)-parent age+19, the last digit of its total calf that is given birth to that breeds during for odd number is male calf, and the last digit of its total calf that is given birth to that breeds during for even number is female cattle calf;
In the formula: the parent age was as the criterion with the copulation reproductive age, and a breeding month umber is as the criterion with the lunar calendar.
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Publication number | Priority date | Publication date | Assignee | Title |
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CN104255653A (en) * | 2014-09-16 | 2015-01-07 | 重庆东奇畜禽养殖有限公司 | Beef improvement technology |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1494824A (en) * | 2002-02-02 | 2004-05-12 | 王桂军 | Cattle directional lemale-male breeding method solution |
CN1524411A (en) * | 2003-09-16 | 2004-09-01 | 汪家玲 | Method for sex determination of cow calving |
CN1593396A (en) * | 2004-06-25 | 2005-03-16 | 王德 | Liquor for increasing cow born ratio and its manufacturing method |
CN101322489A (en) * | 2008-08-01 | 2008-12-17 | 内蒙古蒙牛乳业(集团)股份有限公司 | Deer X/Y sperm separation freezing sperm and producing method thereof and use |
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- 2008-12-29 CN CN200810148078A patent/CN101766507A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1494824A (en) * | 2002-02-02 | 2004-05-12 | 王桂军 | Cattle directional lemale-male breeding method solution |
CN1524411A (en) * | 2003-09-16 | 2004-09-01 | 汪家玲 | Method for sex determination of cow calving |
CN1593396A (en) * | 2004-06-25 | 2005-03-16 | 王德 | Liquor for increasing cow born ratio and its manufacturing method |
CN101322489A (en) * | 2008-08-01 | 2008-12-17 | 内蒙古蒙牛乳业(集团)股份有限公司 | Deer X/Y sperm separation freezing sperm and producing method thereof and use |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104255653A (en) * | 2014-09-16 | 2015-01-07 | 重庆东奇畜禽养殖有限公司 | Beef improvement technology |
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