CN101762646B - Method for measuring content of lactitol - Google Patents
Method for measuring content of lactitol Download PDFInfo
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- CN101762646B CN101762646B CN 200810185072 CN200810185072A CN101762646B CN 101762646 B CN101762646 B CN 101762646B CN 200810185072 CN200810185072 CN 200810185072 CN 200810185072 A CN200810185072 A CN 200810185072A CN 101762646 B CN101762646 B CN 101762646B
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Abstract
The invention provides a method for measuring the content of lactitol, which adopts a high performance liquid internal standard method to carry out the measuring. The method comprises the following steps: (1) preparing a lactitol sample to be measured, and adding the lactitol sample to arabinose solution, so as to dissolve and dilute the lactitol sample; (2) analyzing the lactitol sample to be measured by HPLC, wherein carbohydrate analytical columns are selected as chromatographic columns, calcium-ion coordination exchanger is adopted as padding, water is adopted as a mobile phase, arabinose is adopted as the internal standard, the temperature of the columns is 90 DEG C, the flow rate is 0.5mL/min, a differential detector is adopted, the sample injection volume is 20mul, and the theoretical plate number is larger than or equal to 3,000. The invention has the advantages of accurate measuring, convenient operation, high specificity and high sensitivity.
Description
Technical field
The present invention relates to a kind of chromatogram monitoring method, be specifically related to a kind of method of high effective liquid chromatography for measuring content of lactitol.
Background technology
Lactitol (having another name called: lactitol or lactitolum monohydrate) is the disaccharide derivant that is made up of sorbierite and galactose, not by disaccharidase hydrolysis in the intestines and stomach, and gets into colon with original shape.In colon, be degraded to short chain organic acid (being mainly acetate, propionic acid and butyric acid) by intestinal flora (mainly being bacteroid and lactobacillus), the acidifying colonic contents, thus reduced the absorption of colon to ammonia.These article can be converted into low molecular weight organic acid, cause osmotic pressure rising in the colon, thereby increase the water cut and the volume of ight soil, produce laxative effective.Because these article mouthfeel is fresh and sweet, once once is being used as the sweetener in the food additives.In the provincial standard of Shanghai City; Announced that the employing oxidimetry measures its content; But because the initiation material of preparation Lactitol is a lactose; Intermedium sweet mellow wine, sorbierite, galactitol all can be participated in redox reaction, easily assay are caused interference, influence last testing result; And also there are some shortcomings of common chemical method in this method, such as the visual error of more loaded down with trivial details, the terminal point determining of operation etc.; Therefore utilize oxidimetry to measure content of lactitol, it is inaccurate to have testing result, the specificity of method, reappearance, recovery rate variance, complex operation, shortcomings such as visual error.
Summary of the invention
In order to overcome above-mentioned shortcoming, the present invention adopts high performance liquid chromatography to measure the content that contains Lactitol in the Lactitol sample first.
The invention provides a kind of method of measuring content of lactitol, it is to adopt high performance liquid chromatography to carry out the mensuration of content of lactitol.The object of the invention can realize through following experimental procedure:
(1) preparation of Lactitol sample to be measured is got the Lactitol sample and is added the arabinose WS, dissolving, dilution.
(2) with high performance liquid chromatography Lactitol sample to be measured is analyzed, wherein,
Chromatographic column: carbohydrate analysis column, filler are calcium ion type coordination permutoid;
Moving phase: water;
Interior mark: arabinose;
Column temperature: 90 ℃;
Flow velocity: 0.5mL/min;
Detecting device: differential detecting device;
Sampling volume: 20 μ l;
Theoretical cam curve >=3000.
Wherein, the preparation method of arabinose internal standard compound is to be diluted with water to the solution that every mL contains 10-500mg, standard specimen article in making.
The invention has the advantages that: measure accurate, easy and simple to handle, specificity is strong, highly sensitive.
1. the superiority of instrumental analysis: the core of red, orange, green, blue, yellow (ROGBY) is can component to be measured be separated earlier with other component, post analysis, and the present invention has used suitable chromatographic resolution system, Lactitol and internal standard compound arabinose and all the other components ability good separation.Guaranteed the accurately quantitative of characteristic peak, the analytical approach of this instrumentation is compared with chemical analysis, and specificity is strong, and sensitivity improves greatly.
2. this assay method also has easy and simple to handlely, analyzes characteristics fast.
3. this assay method has adopted internal standard method, and subtle change and the operational accidental error of variation, chromatographic condition of having avoided sample size is to the influence of quantitative result.
Description of drawings
Fig. 1 is the HPLC collection of illustrative plates of lactitolum monohydrate reference substance and internal standard compound matter, and retention time is that the absorption peak of 16.379min is the lactitolum monohydrate reference substance, and retention time is that the absorption peak of 18.593min is an internal standard compound matter.
Embodiment
Embodiment 1
Precision takes by weighing lactitolum monohydrate and each intermedium 250mg, and the arabinose WS with 2% is diluted to 5mg/ml, and precision is measured 20 μ l samples and analyzed, and the content of lactitol that obtains wherein is 99.5%.
Embodiment 2
Precision takes by weighing the about 0.1g of lactitolum monohydrate reference substance, puts in the 5ml measuring bottle, and with 2% arabinose WS dilution, constant volume shakes up, and precision is measured sample solution 20 μ l samples and analyzed, and the content of lactitol that obtains wherein is 99.8%.
The above is merely an embodiment of the present invention, and this embodiment does not only produce any constraint to the present invention as explanation.
Claims (2)
1. method of measuring content of lactitol is characterized in that may further comprise the steps:
(1) preparation of Lactitol sample to be measured is got the Lactitol sample and is added the arabinose WS, dissolving, dilution;
(2) with high performance liquid chromatography Lactitol sample to be measured is analyzed, wherein,
Chromatographic column: carbohydrate analysis column, filler are calcium ion type coordination permutoid;
Moving phase: water;
Interior mark: arabinose;
Column temperature: 90 ℃;
Flow velocity: 0.5mL/min;
Detecting device: differential detecting device;
Sampling volume: 20 μ l;
Theoretical cam curve >=3000.
2. assay method according to claim 1 is characterized in that: the preparation method of arabinose internal standard compound is to be diluted with water to the solution that every mL contains 10～500mg, standard specimen article in making.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810185072 CN101762646B (en) | 2008-12-26 | 2008-12-26 | Method for measuring content of lactitol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810185072 CN101762646B (en) | 2008-12-26 | 2008-12-26 | Method for measuring content of lactitol |
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| CN101762646A CN101762646A (en) | 2010-06-30 |
| CN101762646B true CN101762646B (en) | 2012-09-05 |
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| CN 200810185072 Active CN101762646B (en) | 2008-12-26 | 2008-12-26 | Method for measuring content of lactitol |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI629479B (en) * | 2013-09-30 | 2018-07-11 | 養樂多本社股份有限公司 | Detection and quantification method of galactooligosaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2466030A1 (en) * | 2001-11-07 | 2003-05-15 | Pharmacia Corporation | Intraorally disintegrating valdecoxib compositions prepared by fluid bed granulation process |
| JP3987563B2 (en) * | 2006-10-23 | 2007-10-10 | 第一三共株式会社 | Peptides and their uses |
| JP3987562B2 (en) * | 2006-10-23 | 2007-10-10 | 第一三共株式会社 | Peptides and their uses |
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2008
- 2008-12-26 CN CN 200810185072 patent/CN101762646B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2466030A1 (en) * | 2001-11-07 | 2003-05-15 | Pharmacia Corporation | Intraorally disintegrating valdecoxib compositions prepared by fluid bed granulation process |
| JP3987563B2 (en) * | 2006-10-23 | 2007-10-10 | 第一三共株式会社 | Peptides and their uses |
| JP3987562B2 (en) * | 2006-10-23 | 2007-10-10 | 第一三共株式会社 | Peptides and their uses |
Non-Patent Citations (4)
| Title |
|---|
| 刘文庸，徐立平，张卫东，万维勤.乳糖醇的HPLC-蒸发光散射检测器测定.《中国医药工业杂志》.2001,第32卷(第9期),414-415. * |
| 陈悦 |
| 陈悦;陈青俊.HPLC示差折光分析法测定乳糖醇的含量.《西北药学杂志》.1997,第12卷(第4期),147-148. * |
| 陈青俊.HPLC示差折光分析法测定乳糖醇的含量.《西北药学杂志》.1997,第12卷(第4期),147-148. |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI629479B (en) * | 2013-09-30 | 2018-07-11 | 養樂多本社股份有限公司 | Detection and quantification method of galactooligosaccharide |
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| CN101762646A (en) | 2010-06-30 |
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Inventor after: Luo Rong Inventor after: Lu Hongguo Inventor after: Zhu Lingling Inventor after: Huang Shuping Inventor after: Gu Gaoqiang Inventor before: Lu Hongguo Inventor before: Zhu Lingling Inventor before: Huang Shuping Inventor before: Gu Gaoqiang |
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Free format text: CORRECT: INVENTOR; FROM: LU HONGGUO ZHU LINGLING HUANG SHUPING GU GAOQIANG TO: LUO RONG LU HONGGUO ZHU LINGLING HUANG SHUPING GU GAOQIANG |
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Effective date of registration: 20160616 Address after: 225321 Taizhou Jiangnan Road, No. 1, Jiangsu, China Patentee after: Yangtze River Pharmaceutical Co., Ltd. Patentee after: Jiangsu Haici Biological Pharmaceutical Co., Ltd. of Yangtze River Pharmaceutical Group Address before: 225321 Yangtze River Jiangnan Road, Taizhou, Zhejiang Province, the Yangtze River, the Yangtze River Pharmaceutical Group Co., Ltd. No. 1, Jiangsu Patentee before: Yangtze River Pharmaceutical Co., Ltd. |