CN101759618A - Method for extracting lutein ester compounds form marigolds - Google Patents
Method for extracting lutein ester compounds form marigolds Download PDFInfo
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- CN101759618A CN101759618A CN200810184478A CN200810184478A CN101759618A CN 101759618 A CN101759618 A CN 101759618A CN 200810184478 A CN200810184478 A CN 200810184478A CN 200810184478 A CN200810184478 A CN 200810184478A CN 101759618 A CN101759618 A CN 101759618A
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- ester compounds
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Abstract
The invention discloses a method for extracting lutein ester compounds form marigolds, relating to an extraction method for extracting natural and edible uranidin lutein ester compounds from dried flower particles of the marigolds by adopting column chromatography and comprising two steps of ultrasonic extraction and the column chromatography. The extraction rate of the obtained lutein ester compounds can achieve 8.4%-8.6%, the lutein ester compounds have favorable purity after being determined by ultraviolet spectrophotometry, and the color scale E474nm1% is approximate to 698.
Description
Technical field
The present invention relates to extract in a kind of dried marigold flower pellets the method for lutein ester compounds.
Background technology
Flower of Aztec Marigold (Tagetes erecta L.) belongs to composite family (Compositae) Tagetes (Tagetes Linn.) plant, is one of the abundantest source of edible natural usefulness yellow pigment lutein ester compounds.The lutein ester compounds is the compound of a class long-chain polyene hydrocarbon.At first, they have good pigmentation, can be applicable in poultry and egg and the beverage.Secondly, they have good antioxygenation, can be developed to uses such as healthcare products.In addition, they also have effects such as anticancer, uvioresistant.At present, the lutein ester compounds has been used in the industries such as healthcare products, food nutrition supplement, fodder additives, tobacco and makeup.In addition, the parent xenthophylls of lutein ester compounds is owing to have complicated steric configuration, and the difficulty of synthetic is bigger, and therefore extraction separation obtains the lutein ester compounds and becomes fast way from plant.About the existing report of the research of from dried marigold flower pellets, extracting the lutein ester compounds, but generally all there are productive rate and the lower problem of purity.
Summary of the invention
The object of the present invention is to provide the technology that adopts column chromatography from dried marigold flower pellets, to extract the lutein ester compounds,
The present invention is achieved through the following technical solutions:
The present invention adopts column chromatography to extract the extracting method of natural edible flavochrome lutein ester compounds from dried marigold flower pellets, and it is made up of supersound extraction and these two steps of column chromatography.
A kind of lutein ester compounds method of extracting from Flower of Aztec Marigold is characterized in that this method may further comprise the steps:
A) preparation of dried marigold flower pellets crude extract
Dried marigold flower pellets is pulverized, crossed 10～20 mesh sieves, after the ethanol of doubly measuring with 10-30 carries out supersound extraction, filter, the dregs of a decoction filter with ethanol supersound extraction once more, merge above-mentioned filtrate, obtain clear filtrate behind the suction filtration,, obtain pale brown look extract at drying under reduced pressure with decompression filtrate recycling ethanol;
B) column chromatography prepares the lutein ester compounds
After the said extracted thing added dissolve with ethanol, to put forward the thing amount: 1: 1～1: 1.5 (weight ratio) mixed of silica gel amount (200～300 order) is even, silicagel column is gone up in back to be dried, silicagel column is interior according to the exsiccant applied sample amount: the ratio of silica gel amount 1: 5～1: 10 (weight ratio) is filled 200～300 purpose silica gel in silicagel column, with an amount of sherwood oil: 3: 1 wash-outs of ethyl acetate, the salmon liquid under the wash-out concentrate and are the lutein ester compounds.
Extraction yield of the present invention can reach 8.4%～8.6%, and ultraviolet spectrophotometer is measured it absorption (with acetone as solvent) in 400～500 scopes, and it is better to be indicated as lutein ester compounds and purity, its look valency E
474nm 1%Be about 698.
Compared with prior art, this method technology is simple, low production cost, and cost pick-up rate height can not destroy effective constituent.The simple column chromatography method of this employing, the extraction yield of the lutein ester compounds that obtains can reach 8.4%～8.6%, and determined by ultraviolet spectrophotometry shows that its purity is better, its look valency E
474nm 1%Be about 698.
Embodiment
Embodiment 1:
Get dried marigold flower pellets, pulverize the back and cross 20 mesh sieves, take by weighing 10.0g, the medical ethanol 200ml of adding 95%, supersound extraction 30min under the room temperature filters, the dregs of a decoction add 95% medical ethanol 200ml, supersound extraction 30min, filter and remove residue again, the dregs of a decoction are extracted once again, merging filtrate, suction filtration obtains clear filtrate, decompression filtrate recycling ethanol to doing, is obtained 2.4g palm fibre xanchromatic at 40 ℃ of drying under reduced pressure and slightly extracts; After 95% the medical ethanol dissolving of above-mentioned crude extract with 20ml, with crude extract weight: 1: 1.3 ratio of silica gel weight (200～300 order) is admixed silica gel and is mixed, silicagel column is gone up in back to be dried, adorn 200～300 purpose silica gel 54g in the silicagel column, use the 900ml sherwood oil: 3: 1 wash-outs of ethyl acetate, salmon liquid under the wash-out concentrates, promptly obtain the lutein ester compounds of 0.855g at 40 ℃ of drying under reduced pressure, yield is 8.55% (in the amount of dried marigold flower pellets), its look valency of spectrophotometry E
474nm 1%Be about 698.
Embodiment 2:
Get dried marigold flower pellets, pulverize the back and cross 20 mesh sieves, take by weighing 20.0g, the medical ethanol 400ml of adding 95%, supersound extraction 30mm under the room temperature filters, the dregs of a decoction add 95% medical ethanol 400ml, supersound extraction 30min, filter and remove residue again, the dregs of a decoction are extracted once again, merging filtrate, suction filtration obtains clear filtrate, decompression filtrate recycling ethanol to doing, is obtained 5.6g palm fibre xanchromatic at 40 ℃ of drying under reduced pressure and slightly extracts; After 95% the medical ethanol dissolving of above-mentioned crude extract with 40ml, with the crude extract amount: 1: 1.3 ratio of silica gel amount (200～300 order) is admixed silica gel and is mixed, silicagel column is gone up in back to be dried, adorn 200～300 purpose silica gel 80g in the silicagel column, use the 900ml sherwood oil: 3: 1 wash-outs of ethyl acetate, salmon liquid under the wash-out is concentrated, promptly obtain 1.168g lutein ester compounds at 40 ℃ of drying under reduced pressure, yield is 8.41% (in the amount of dried marigold flower pellets), its look valency of spectrophotometry E
474nm 1%Be about 696.
Claims (1)
1. one kind is extracted lutein ester compounds method from Flower of Aztec Marigold, it is characterized in that this method may further comprise the steps:
A) preparation of dried marigold flower pellets crude extract
Dried marigold flower pellets is pulverized, crossed 10～20 mesh sieves, after the ethanol of doubly measuring with 10-30 carries out supersound extraction, filter, the dregs of a decoction filter with ethanol supersound extraction once more, merge above-mentioned filtrate, obtain clear filtrate behind the suction filtration,, obtain pale brown look extract at drying under reduced pressure with decompression filtrate recycling ethanol;
B) column chromatography prepares the lutein ester compounds
After the said extracted thing added dissolve with ethanol, to put forward the thing amount: 1: 1～1: 1.5 (weight ratio) mixed of silica gel amount (200～300 order) is even, silicagel column is gone up in back to be dried, silicagel column is interior according to the exsiccant applied sample amount: the ratio of silica gel amount 1: 5～1: 10 (weight ratio) is filled 200～300 purpose silica gel in silicagel column, with an amount of sherwood oil: 3: 1 wash-outs of ethyl acetate, the salmon liquid under the wash-out concentrate and are the lutein ester compounds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810184478A CN101759618A (en) | 2008-12-25 | 2008-12-25 | Method for extracting lutein ester compounds form marigolds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200810184478A CN101759618A (en) | 2008-12-25 | 2008-12-25 | Method for extracting lutein ester compounds form marigolds |
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| CN101759618A true CN101759618A (en) | 2010-06-30 |
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| CN200810184478A Pending CN101759618A (en) | 2008-12-25 | 2008-12-25 | Method for extracting lutein ester compounds form marigolds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102838519A (en) * | 2012-03-13 | 2012-12-26 | 华北制药集团新药研究开发有限责任公司 | Method for separating and purifying all-trans high-purity lutein esters powder |
| CN103992256A (en) * | 2014-05-17 | 2014-08-20 | 昆明涞章医药科技有限公司 | Method for preparing high-purity lutein esters from pot marigold |
| CN108299265A (en) * | 2018-01-17 | 2018-07-20 | 济宁医学院 | A method of extracting zeaxanthin and lutein from corn and its by-product |
| CN110590629B (en) * | 2019-09-29 | 2021-07-13 | 山东天音生物科技有限公司 | Method for separating and purifying lutein from marigold oleoresin |
| CN114957073A (en) * | 2021-02-25 | 2022-08-30 | 内蒙古昶辉生物科技股份有限公司 | Method for preparing lutein ester from marigold ointment and lutein ester |
-
2008
- 2008-12-25 CN CN200810184478A patent/CN101759618A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102838519A (en) * | 2012-03-13 | 2012-12-26 | 华北制药集团新药研究开发有限责任公司 | Method for separating and purifying all-trans high-purity lutein esters powder |
| CN102838519B (en) * | 2012-03-13 | 2014-05-14 | 华北制药集团新药研究开发有限责任公司 | Method for separating and purifying all-trans high-purity lutein esters powder |
| CN103992256A (en) * | 2014-05-17 | 2014-08-20 | 昆明涞章医药科技有限公司 | Method for preparing high-purity lutein esters from pot marigold |
| CN103992256B (en) * | 2014-05-17 | 2016-09-07 | 昆明涞章医药科技有限公司 | A kind of method preparing high-purity lutein ester from pot marigold |
| CN108299265A (en) * | 2018-01-17 | 2018-07-20 | 济宁医学院 | A method of extracting zeaxanthin and lutein from corn and its by-product |
| CN110590629B (en) * | 2019-09-29 | 2021-07-13 | 山东天音生物科技有限公司 | Method for separating and purifying lutein from marigold oleoresin |
| CN114957073A (en) * | 2021-02-25 | 2022-08-30 | 内蒙古昶辉生物科技股份有限公司 | Method for preparing lutein ester from marigold ointment and lutein ester |
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Application publication date: 20100630 |