CN101757688A - Method for making bracket by using fibrin glue - Google Patents
Method for making bracket by using fibrin glue Download PDFInfo
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- CN101757688A CN101757688A CN200810202902A CN200810202902A CN101757688A CN 101757688 A CN101757688 A CN 101757688A CN 200810202902 A CN200810202902 A CN 200810202902A CN 200810202902 A CN200810202902 A CN 200810202902A CN 101757688 A CN101757688 A CN 101757688A
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Abstract
The invention relates to a method for making bracket by using fibrin glue. The method comprises the following steps that: 1) a solution A1 and a solution B1 are respectively prepared; 2) the solutions A1 and B2 with the same amount are rapidly mixed uniformly and then poured into a mould; 3) the mixture is frozen and dried; and 4) then the mixture is exposed to gamma rays for sterilization. The solution A1 contains fibrinogen, carboxymethyl cellulose, aminopeptidase and glycerol in weight proportion of 3-9:1:0.05-0.2:15-25; and the solution B1 contains 1-5 IU/ml of thrombosin and 2-8 mg/ml of calcium chloride. Compared with the prior art, the invention has the advantages that the method is simple, reliable and practical, and the structure can be changed at will.
Description
Technical field
The present invention relates to the animal fibrin glue material, especially relate to a kind of method with Fibrin Glue material construction support.
Background technology
Fibrin Glue is by fiber-rich proteinogen component (Fibrinogen-Rich-Fraction FRF.Wherein mainly contain Fibrinogen, the XIII factor, fibronectin), the biomaterial formed such as thrombin, calcium chloride.Final stage by simulation blood coagulation reaction forms fibrin with the thrombin activation Fibrinogen.Use atomic force fluorescence microscopies such as Liu detect the elastic limit and the producibility of single fiber protein yarn, display fibers albumen has thundering producibility and elasticity, its physical property far is better than spider silk, and this result's publication is on " science " magazine in August, 2006.
The application of fibrin glue material also mainly concentrates on clinical hemostasis aspect at present, and application in other respects also has very big extending space, especially the tissue engineering bracket aspect.Fibrin can promote fibroblast and smooth muscle cell secretory cell epimatrix; Can with numerous cellular matrixs (as fibronectin), somatomedin is (as fibroblast growth factor, vascular endothelial cell growth factor etc.) combination makes it become a kind of " intelligence biological material ", as the initial albumen of a kind of wound healing and tissue repair.Cause gradually that in recent years people more and more pay attention to.The mechanical strength of fibrin colloid, physical characteristics such as extensibility, degradation speed can be regulated by adding thrombin, the XIII factor, calcium ion, fibrinolytic material and other adjuvant; In addition, Fibrin Glue (no matter being human blood source or animal blood source) in the extensive use of surgical clinical, proves that this material and the tissue compatibility are fabulous, not only has abundant raw material sources, and the host does not produce immunoreation to graft, and is safe and reliable.So the application of Fibrin Glue is still waiting to excavate.
Summary of the invention
Purpose of the present invention is exactly to provide a kind of method simple and reliable, the random method of mapped structure, usefulness Fibrin Glue material construction support that application is strong in order to overcome the defective that above-mentioned prior art exists.
Purpose of the present invention can be achieved through the following technical solutions: a kind of method with Fibrin Glue material construction support, it is characterized in that, this method may further comprise the steps: (1) disposes A1 and B1 solution respectively, (2) with A1 and the quick mixing of B1 solution equivalent and pour mould into, (3) lyophilization, the sterilization of (4) gamma-ray irradiation; Described A1 solution comprises that weight ratio is 3～9: 1: 0.05～0.2: 15～25 Fibrinogen, carboxymethyl cellulose, aminopeptidase and glycerol; Described B1 solution is thrombin 1～5IU/ml, calcium chloride 2～8mg/ml.
Described A1 solution comprises that weight ratio is 6: 1: 0.1: 18 Fibrinogen, carboxymethyl cellulose, aminopeptidase and glycerol; Described B1 solution is thrombin 2IU/ml, calcium chloride 4.4mg/ml.
Described A1 and B1 solution mixing and pour mould in 1～3 minute, and after 1～3 minute, from mould, take out.
Described A1 and B1 solution mixing and pour mould in 2 minutes, and after 2 minutes, from mould, take out.
Described lyophilization makes the moisture content of material below 1%.
It is 0.5～1% that described lyophilization makes the moisture content of material.
The gamma-ray irradiation that described gamma-ray irradiation sterilization is 20～30KGY dosage 24～72 hours.
Compared with prior art, the technology that the present invention adopts is simply effective, as long as providing suitable mould all can produce with the fibrin is the support of raw material, be beneficial to the application of Fibrin Glue, fibrinogenic concentration has the adjustment space, can produce fibrin scaffold which of different nature, ray sterilizing technology after the employing lyophilizing, can guarantee that fibrin is not destroyed, this point is the basis that makes tissue engineering bracket.
Description of drawings
Fig. 1 is the mould sketch map of tubular support;
Fig. 2 is freeze-drying curve figure.
The specific embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
Configuration solution:
One, fibrinogen solution 10ml standby (A1), wherein Fibrinogen 30mg/ml, carboxyl methyl cellulose 5mg/ml, Aminopeptidase A PN 0.5mg/ml, glycerol 90mg/ are every part.Two, prepare catalyst solution (B1) 10ml, thrombin 2IU/ml in the B1 solution, calcium chloride 4.4mg/ml is standby.
The preparation gel tube:
As shown in Figure 1, after A1 solution and the stirring of B1 solution mixing, the glass sock 1 and glass rod 2 external members of the rapid good skin plug 3 of filler plug.
Lyophilizing: carefully take off gel tube and put into ready plate, lyophilizing, freeze-drying curve is as shown in Figure 2.
Sterilization: adopt 3 layers of plastic bag that freeze-dried material is sealed, irradiation sterilization is 24 hours under 20-30KGY dosage.
Embodiment 2
A kind of method with Fibrin Glue material construction support, this method may further comprise the steps: (1) disposes A1 and B1 solution respectively, (2) with A1 and B1 solution equivalent mixing and pour mould in 1 minute, and after 1 minute, from mould, take out, (3) lyophilization, the moisture content that makes material is 0.5%, and (4) adopt the gamma-ray irradiation sterilization in 72 hours of 20KGY dosage; Described A1 solution comprises that weight ratio is 3: 1: 0.05: 15 Fibrinogen, carboxymethyl cellulose, aminopeptidase and glycerol; Described B1 solution is thrombin 1IU/ml, calcium chloride 2mg/ml.
Embodiment 3
A kind of method with Fibrin Glue material construction support, this method may further comprise the steps: (1) disposes A1 and B1 solution respectively, (2) with A1 and B1 solution equivalent mixing and pour mould in 3 minutes, and after 3 minutes, from mould, take out, (3) lyophilization, the moisture content that makes material is 1%, and (4) adopt the gamma-ray irradiation sterilization in 36 hours of 20KGY dosage; Described A1 solution comprises that weight ratio is 9: 1: 0.2: 25 Fibrinogen, carboxymethyl cellulose, aminopeptidase and glycerol; Described B1 solution is thrombin 5IU/ml, calcium chloride 8mg/ml.
Claims (7)
1. method with Fibrin Glue material construction support, it is characterized in that this method may further comprise the steps: (1) disposes A1 and B1 solution respectively, and (2) are with A1 and the quick mixing of B1 solution equivalent and pour mould into, (3) lyophilization, the sterilization of (4) gamma-ray irradiation; Described A1 solution comprises that weight ratio is 3～9: 1: 0.05～0.2: 15～25 Fibrinogen, carboxymethyl cellulose, aminopeptidase and glycerol; Described B1 solution is thrombin 1～5IU/ml, calcium chloride 2～8mg/ml.
2. a kind of method with Fibrin Glue material construction support according to claim 1 is characterized in that described A1 solution comprises that weight ratio is 6: 1: 0.1: 18 Fibrinogen, carboxymethyl cellulose, aminopeptidase and glycerol; Described B1 solution is thrombin 2IU/ml, calcium chloride 4.4mg/ml.
3. a kind of method with Fibrin Glue material construction support according to claim 1 is characterized in that, described A1 and B1 solution mixing and pour mould in 1～3 minute, and after 1～3 minute, from mould, take out.
4. a kind of method with Fibrin Glue material construction support according to claim 3 is characterized in that, described A1 and B1 solution mixing and pour mould in 2 minutes, and after 2 minutes, from mould, take out.
5. a kind of method with Fibrin Glue material construction support according to claim 1 is characterized in that described lyophilization makes the moisture content of material below 1%.
6. a kind of method with Fibrin Glue material construction support according to claim 1 is characterized in that it is 0.5～1% that described lyophilization makes the moisture content of material.
7. a kind of method with Fibrin Glue material construction support according to claim 1 is characterized in that, the gamma-ray irradiation that described gamma-ray irradiation sterilization is 20～30KGY dosage 24～72 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810202902A CN101757688A (en) | 2008-11-18 | 2008-11-18 | Method for making bracket by using fibrin glue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810202902A CN101757688A (en) | 2008-11-18 | 2008-11-18 | Method for making bracket by using fibrin glue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101757688A true CN101757688A (en) | 2010-06-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810202902A Pending CN101757688A (en) | 2008-11-18 | 2008-11-18 | Method for making bracket by using fibrin glue |
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| CN (1) | CN101757688A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105705172A (en) * | 2013-11-19 | 2016-06-22 | 上海松力生物技术有限公司 | Hydrophilic electrospinning biological composite stent material used for tissue regeneration and preparation method and application thereof |
-
2008
- 2008-11-18 CN CN200810202902A patent/CN101757688A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105705172A (en) * | 2013-11-19 | 2016-06-22 | 上海松力生物技术有限公司 | Hydrophilic electrospinning biological composite stent material used for tissue regeneration and preparation method and application thereof |
| CN105705172B (en) * | 2013-11-19 | 2021-12-03 | 上海松力生物技术有限公司 | Hydrophilic electrostatic spinning biological composite scaffold material for tissue regeneration and preparation method and application thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Open date: 20100630 |