CN101755884A - Process and device for sterile blood sampling of live pigs - Google Patents
Process and device for sterile blood sampling of live pigs Download PDFInfo
- Publication number
- CN101755884A CN101755884A CN200810202905A CN200810202905A CN101755884A CN 101755884 A CN101755884 A CN 101755884A CN 200810202905 A CN200810202905 A CN 200810202905A CN 200810202905 A CN200810202905 A CN 200810202905A CN 101755884 A CN101755884 A CN 101755884A
- Authority
- CN
- China
- Prior art keywords
- blood sampling
- pig
- blood
- live
- sterile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The invention relates to a process and a device for sterile blood sampling of live pigs. The process comprises: fixing a live pig in a sterilized hermetic blood sampling system; puncturing the heart the live pig and the great vessel connected with the heart through a puncture needle to perform sterile blood sampling; and connecting the puncture needle with a blood collection container through a duct to collect blood, wherein the blood sampling speed is 0.5 to 5 L/1 to 10 minutes, and the blood sampling amount is 0.5 to 5 L/100 kg/pig. Compared with the prior art, the process has the advantages of simplicity, easy implementation, effective prevention of microbial contamination to pig blood, low cost and the like.
Description
Technical field
The present invention relates to the animal fibrin glue material, especially relate to a kind of sterile blood sampling of live pigs technology and device.
Background technology
Pig blood is a kind of good resource.Whole blood contains protein 18%, and wherein blood plasma accounts for 65% of whole blood, contains protein 8%, and red blood cell partly accounts for 35% of whole blood, and protein content is 36%.Extract fibrinogen and fibrin ferment in the pig blood, after the series of process processing, can substitute human blood, be prepared into effective styptic---Fibrin Glue, can stop wild oozing of blood of operation art and tiny phleborrhagia with getting instant result, simultaneously surgical wound surface is implemented effectively tissue sealing, reduce oozing out of tissue fluid effectively, accelerate wound healing.A large amount of clinical application research results confirms the validity and the security of this product.
Pig blood is the biomaterial for medical purpose that raw material is made, and its maximum risk is biological risk. wherein microbial contamination is most important.
Pig slaughtering generally adopts the assassination depletion method at present.After live pig caused dusk by physics (electricity or electromagnetic wave stunning method) or chemistry (carbon dioxide process) means, fleam inserted heart of pig and trunk on every side thereof, allows the blood nature flow out from the edge of a knife; Blood flow is gone into the blood pond or is got blood container by the blood concentrated flow.Because by pollutions such as the addle of hair, health and mouth and nose liquid, the blood sanitary index is very poor through knife-edge part for the pig blood flow.
As everyone knows, blood is the very complicated biochemical system of composition.Very stable in vivo under the normal physiological situation, unstable after exsomatizing, very fast generation series of biochemical reactions is as blood coagulation, haemolysis, oxidation etc.Because be good nutrient matrix, be easy to be subjected to contamination by micro again, breeding fast, thus make pig blood rotten, lose value.
In the production process of Fibrin Glue, micro organism quantity how to control raw material and intermediate products effectively is very important.Such as, we adopt the final sterilization mode of gamma rays as aseptic Fibrin Glue main body powder, how to determine the dosage of gamma rays, guarantee that to reach it (is the horizontal SAL of aseptic assurance 〉=10 that product meets the sterilization requirement
-6), closely related with the content of microorganisms of per unit sample in the sample subject to sterilization.If we adopt the irradiation dose of 25KGy to sterilize, requirement according to EN552:1994 (Sterilization of medical devices-validationand routine control of sterilization by irradiation), the sample of every least unit, bacterial content can not be greater than 100cfu (clone form unit clonogenic unit).In fact, if we do not apply effective control measure, utilize and assassinate the pig blood that depletion method obtained, after a production cycle, the content of microorganisms (cfu) of predose intermediate products per unit sample can be up to up to ten million.
How the blood supply pig is implemented sterile blood sampling effectively, thereby reduce the quantity of microbial contamination in the raw material blood as far as possible, do not see open source information both at home and abroad.
The preparation that relevant employing asepsis is carried out cow's serum has following open report.Li Yong discloses " with the method for multi-joint bag aseptic production of animal raw serum in full sealing environment (Chinese patent application number 200510096345.2).Patent is described and is adopted blood taking needle to be connected with the blood bag with conduit by three-way pipe, blood taking needle inserts in the blood vessel of ox neck, and ox blood is introduced blood bag, the separation that the promoting circulation of blood of going forward side by side is clear by conduit, this invention has realized carrying out the production of aseptic animal raw serum in same sealing system.Ma Zhongren etc. have reported a kind of manufacturing technique of fetal bovine serum.This technology comprises full closed aseptic collection, cryogenic separation, micro-pore-film filtration.Be characterized in: conceived about 8 months cow is carried out caesarean birth, antenatal employing Baoding method of lying on one's side, the method for general anesthesia and local narcotic phase combination is adopted in anesthesia.Tire Niu Jinhang full closed aseptic cardiac puncture is taken a blood sample.(Chinese patent application number: 200610104473.1).
Summary of the invention
Purpose of the present invention be exactly provide in order to overcome the defective that above-mentioned prior art exists a kind of simple and easy to do, prevent the pollution of microorganism to pig blood, sterile blood sampling of live pigs technology and device that cost is low effectively.
Purpose of the present invention can be achieved through the following technical solutions: a kind of sterile blood sampling of live pigs technology, it is characterized in that, this technology is that live pig is fixed in the airtight blood sampling system after the sterilization, by puncture needle the heart of live pig and the trunk that links to each other with heart are punctured and to carry out sterile blood sampling, and puncture needle is connected with blood collection container with conduit, blood is collected, and blood sampling speed is 0.5～5L/1～10 minute, and blood sampling volume is a 0.5～5L/100kg/ pig.
Described blood sampling speed is 2～3L/1～4 minute, and blood sampling volume is a 2～3L/100kg/ pig.
Moist heat sterilization is adopted in the sterilization of described airtight blood sampling system, hot air sterilization, and EOG gaseous sterilization or gamma ray are sterilized, and all need sterilize before the each use of airtight blood sampling system.
Described puncture needle is tubular puncture needle, and its diameter is 0.2～6cm, and length is 5～100cm, and making material is metal or glass, pottery, or hard macromolecular material, and described tubular puncture needle is disposable use, and is capable of washing, and bears sterilizing methods and handle.
The diameter of described tubular puncture needle is 4～5cm, and length is 50～70cm, and the making material is a stainless steel, copper, iron or plastics, preferred 316L stainless steel.
The point of puncture of described puncture needle is a pig when uprightly carrying on the back the chest position, the arbitrfary point of thorax body surface, and preferred breastbone, the formed body surface of first rib and clavicle trigonum, described trunk comprises sustainer, pulmonary vein, pulmonary artery, vena cava anterior.
Described blood collection container material stable by chemical property, that can stand strong acid and highly basic is made, can bear sterilizing methods handles, and be disposable use, described conduit material stable by chemical property, that can stand strong acid and highly basic is made, can bear sterilizing methods and handle, and be disposable use.
The preparation material of described blood collection container includes but not limited to glass, plastics, and metal, the preparation material of described conduit includes but not limited to glass, plastics, metal.
The fixture that a kind of sterile blood sampling of live pigs technology is used, it is characterized in that, this fixture comprises hinge, track and folder pig device, and described hinge hangs upside down live pig in orbit, and described folder pig device is fixed on the frame, folder pig device has a plurality of, each folder pig device is a station, and each station has two tracks of live pig turnover, and live pig enters the station blood sampling by a track, after blood sampling finishes, transport station by another track.
Described folder pig device adopts electronic, pneumatic or mechanical as power, and to control its folding, described fixture is wholy set in the airtight blood sampling system.
The present invention is intended to design sterile blood sampling device and the technology of a cover at the blood supply pig, successfully obtains aseptic pig blood by this invention, and this pig blood will be as producing biomaterial for medical purpose---the acceptable material of Fibrin Glue.Compared with prior art, have the following advantages:
1, simple and easy to do:
The present invention is based on that pig slaughtering is assassinated the bloodletting technology and the sterile blood sampling technology that develops.Because adopt folder pig device to hang upside down, the blood supply pig of having hit unconsciously is fixed, the blood sampling operator is easy to the blood sampling point is cleaned and sterilizes, and also is easy to determine point of puncture, thereby brings great convenience for aseptic puncture and blood sampling.
2, prevent the pollution of microorganism effectively to pig blood
Owing to adopted tubular puncture needle, the closed system that airtight blood collection container and corresponding conduit are formed is as long as the operator through screwing up discipline according to the operation of blood sampling S.O.P., just can reduce contamination by micro in the blood collection procedure to the utmost.
3, with the pig slaughtering line locking, greatly reduce investment intensity
The pig slaughtering streamline comprises following key link 1) kill before shower, 2) electric fiber crops hit dizzy, 3) assassinate blood sampling, 4) depilation, 5) ketoboidies and internal organ verification of synchronization, 6) cool off acid discharge.This sterile blood sampling device can be used as pig slaughtering streamline 3) assassinate the blood sampling link, can not exert an influence to whole piece pig slaughtering continuous productive process process, thereby greatly reduce investment.
Description of drawings
Fig. 1 is the schematic diagram of live pig fixture;
Fig. 2 is the schematic diagram of folder pig device.
The specific embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
Preparation before the blood sampling: open uviol lamp between blood-collecting car, to whole workshop sterilization 30 minutes, sterilization was turned off uviol lamp after finishing.Check whether water power is normal; Start trial run elevator one circle.The fixed position, workshop is uncarted and be placed on to the blood sampling instrument that sterilization is good.
Wash one's hands: earlier the hand water is drenched, smears soap again, then both hands wash palm, the back of the hand, wrist repeatedly by rubbing with the hands, refer between and nail, until a large amount of foams occurring; Water flushing both hands are rubbed with the hands while washing again, do not have soapy feeling until both hands.Put on mouth mask, gloves.
Hand is carried out disinfection: the watering can of 75% alcohol is housed in using, and facing to the hand spray, the palm of every hand and the back of the hand spray 3 times.
The tool car table top is sterilized: with 75% alcohol wipe tool car table top.
The blood sampling instrument: each blood supply pig is adopted the cover blood sampling instrument through aseptic process.This cover instrument comprises: 5000ml blood collecting bottle (be with plug, advance blood vessel and blast pipe), advance silicone tube and the blood sampling knife that blood vessel links to each other with blood collecting bottle, scalpel, cloth bag and stainless steel basket.Blood collecting bottle places tool car lower floor, and remainder places the upper strata.
Blood sampling: record is by the ear tag numbering of blood sampling pig.
The sterilization of blood sampling site: after pig is fixed, be the center with predetermined blood sampling entry point, to diameter 10cm scope, 0.05% Iodophor part is even sprayed 10 times and is carried out disinfection.This process repeats at least twice.
Predetermined blood sampling entry point: in oxter, a pig left side: go up apart from clavicular line 3-4cm, right apart from tracheae line 2-3cm place.The skin incision 1.5-2cm that opens 1-1.5cm with the aseptic operation cutter along parallel side with tracheae is long, exposes hypodermis.With right hand forefinger and middle finger fastening blood sampling knife, palm withstands the blood sampling knife bottom, and holds with a firm grip.Palmar aspect is to oneself, and the back of the hand gently pastes the pig body, and the point of a knife of blood sampling knife inserts skin incision.Knife bar and tracheae line parallel, and with the angle of veritcal linearity into about 30-35 °.Firmly blood sampling knife being boosted, 14.2cm is the blood sampling knife first screw thread place in the pig health.Forefinger, middle finger and thumb clamp blood sampling knife, and palm unclamps the blood sampling knife bottom, and axle and cutter head slide into the end downwards by gravity in the blood sampling knife.Blood with blood sampling knife sidepiece draining hole in silica gel catheter flows to blood collecting bottle.After blood flow is most, with the silicone tube of stainless steel clamp two-fold.Extract blood sampling knife. scalpel, blood sampling knife are rinsed well to be put in reclaim in the instrument cases.
The flushing both hands, the bloodstain that may speckle with is rinsed well.The blood collecting bottle that blood is housed is wrapped, and sticked the label that has filled, be transported on the lorry then.Send clean room to be for further processing.Three batches of sterile blood sampling statistical analyses as a result see Table 1.
Three batches of sterile blood sampling result statistics of table 1
Embodiment 2
Shown in Fig. 1～2, a kind of sterile blood sampling of live pigs technology is clamped live pig, and is hung upside down on track 2 by hinges 1 with folder pig device 3, folder pig device 3 is fixed on the frame 4, folder pig device has a plurality of, and folder pig device adopts electric cylinder 5 its foldings of control, and each folder pig device is a station, each station has two tracks of live pig turnover, live pig enters the station blood sampling by a track, after blood sampling finishes, transports station by another track.In the whole airtight blood sampling system that is fixed in after the sterilization of the fixture that accompanies live pig, by puncture needle the heart of live pig and the trunk that links to each other with heart are punctured and to carry out sterile blood sampling, moist heat sterilization is adopted in the sterilization of this airtight blood sampling system, and puncture needle is connected with blood collection container with conduit, blood is collected, blood sampling speed is 0.5～5L/1～10 minute, and blood sampling volume is a 0.5～5L/100kg/ pig.Described puncture needle is tubular puncture needle, and diameter is 0.2～6cm, and length is 5～100cm, and making material is the 316L stainless steel, and tubular puncture needle is disposable use, and is capable of washing, and can bear above-mentioned moist heat sterilization and handle.Blood collection container glass material stable by chemical property, that can stand strong acid and highly basic is made, can bear sterilizing methods handles, and be disposable use, described conduit plastic material stable by chemical property, that can stand strong acid and highly basic is made, can bear sterilizing methods and handle, and be disposable use.
In the whole airtight blood sampling system that is fixed in after the sterilization of the fixture that accompanies live pig, by puncture needle the heart of live pig and the trunk that links to each other with heart are punctured and to carry out sterile blood sampling, moist heat sterilization is adopted in the sterilization of this airtight blood sampling system, and puncture needle is connected with blood collection container with conduit, blood is collected, blood sampling speed is 2～3L/1～4 minute, and blood sampling volume is a 2～3L/100kg/ pig.Described puncture needle is tubular puncture needle, and its diameter is 4～5cm, and length is 50～70cm, making material is that chemical property glass material stable, that can stand strong acid and highly basic is made, tubular puncture needle is disposable use, and is capable of washing, and can bear above-mentioned moist heat sterilization and handle.Blood collection container plastic material stable by chemical property, that can stand strong acid and highly basic is made, can bear sterilizing methods handles, and be disposable use, described conduit plastic material stable by chemical property, that can stand strong acid and highly basic is made, can bear sterilizing methods and handle, and be disposable use.
The point of puncture of puncture needle is a pig when uprightly carrying on the back the chest position, the arbitrfary point of thorax body surface, and preferred breastbone, the formed body surface of first rib and clavicle trigonum, described trunk comprises sustainer, pulmonary vein, pulmonary artery, vena cava anterior.
The folder pig device of the fixture of above-mentioned live pig can adopt electronic, and is pneumatic or mechanical as power, to control its folding.
Claims (10)
1. sterile blood sampling of live pigs technology, it is characterized in that, this technology is that live pig is fixed in the airtight blood sampling system after the sterilization, by puncture needle the heart of live pig and the trunk that links to each other with heart are punctured and to carry out sterile blood sampling, and puncture needle is connected with blood collection container with conduit, blood is collected, and blood sampling speed is 0.5～5L/1～10 minute, and blood sampling volume is a 0.5～5L/100kg/ pig.
2. a kind of sterile blood sampling of live pigs technology according to claim 1 is characterized in that, described blood sampling speed is 2～3L/1～4 minute, and blood sampling volume is a 2～3L/100kg/ pig.
3. a kind of sterile blood sampling of live pigs technology according to claim 1, it is characterized in that moist heat sterilization, hot air sterilization are adopted in the sterilization of described airtight blood sampling system, EOG gaseous sterilization or gamma ray are sterilized, and all need sterilize before the each use of airtight blood sampling system.
4. a kind of sterile blood sampling of live pigs technology according to claim 1, it is characterized in that, described puncture needle is tubular puncture needle, and its diameter is 0.2～6cm, and length is 5～100cm, making material is metal or glass, pottery, or hard macromolecular material, described tubular puncture needle is disposable use, capable of washing, and bear sterilizing methods and handle.
5. a kind of sterile blood sampling of live pigs technology according to claim 4 is characterized in that the diameter of described tubular puncture needle is 4～5cm, and length is 50～70cm, and the making material is a stainless steel, copper, iron or plastics, preferred 316L stainless steel.
6. a kind of sterile blood sampling of live pigs technology according to claim 1, it is characterized in that, the point of puncture of described puncture needle is that pig is when uprightly carrying on the back the chest position, the arbitrfary point of thorax body surface, preferred breastbone, the formed body surface of first rib and clavicle trigonum, described trunk comprises sustainer, pulmonary vein, pulmonary artery, vena cava anterior.
7. a kind of sterile blood sampling of live pigs technology according to claim 1, it is characterized in that, described blood collection container material stable by chemical property, that can stand strong acid and highly basic is made, can bear sterilizing methods handles, and be disposable use, described conduit material stable by chemical property, that can stand strong acid and highly basic is made, and can bear sterilizing methods and handle, and be disposable use.
8. a kind of sterile blood sampling of live pigs technology according to claim 7 is characterized in that the preparation material of described blood collection container includes but not limited to glass, plastics, and metal, the preparation material of described conduit includes but not limited to glass, plastics, metal.
9. fixture that the described sterile blood sampling of live pigs technology of claim 1 is used, it is characterized in that, this fixture comprises hinge, track and folder pig device, and described hinge hangs upside down live pig in orbit, and described folder pig device is fixed on the frame, folder pig device has a plurality of, each folder pig device is a station, and each station has two tracks of live pig turnover, and live pig enters the station blood sampling by a track, after blood sampling finishes, transport station by another track.
10. the fixture that sterile blood sampling of live pigs technology according to claim 9 is used is characterized in that, described folder pig device adopts electronic, pneumatic or mechanical as power, and to control its folding, described fixture is wholy set in the airtight blood sampling system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810202905A CN101755884A (en) | 2008-11-18 | 2008-11-18 | Process and device for sterile blood sampling of live pigs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810202905A CN101755884A (en) | 2008-11-18 | 2008-11-18 | Process and device for sterile blood sampling of live pigs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101755884A true CN101755884A (en) | 2010-06-30 |
Family
ID=42487389
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810202905A Pending CN101755884A (en) | 2008-11-18 | 2008-11-18 | Process and device for sterile blood sampling of live pigs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101755884A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222496A (en) * | 2013-04-16 | 2013-07-31 | 庞作仁 | Pumped and collected waterfowl blood production system and process |
| CN108782695A (en) * | 2018-08-01 | 2018-11-13 | 漯河双汇肉业有限公司 | A kind of white dirty line hitch gear |
| CN111771942A (en) * | 2020-07-11 | 2020-10-16 | 安徽聚丰畜禽开发有限公司 | Full-automatic slaughter house that stabs and kills is with small-size device of stabbing |
-
2008
- 2008-11-18 CN CN200810202905A patent/CN101755884A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222496A (en) * | 2013-04-16 | 2013-07-31 | 庞作仁 | Pumped and collected waterfowl blood production system and process |
| CN108782695A (en) * | 2018-08-01 | 2018-11-13 | 漯河双汇肉业有限公司 | A kind of white dirty line hitch gear |
| CN111771942A (en) * | 2020-07-11 | 2020-10-16 | 安徽聚丰畜禽开发有限公司 | Full-automatic slaughter house that stabs and kills is with small-size device of stabbing |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106580344B (en) | The Venous Sampling Methods of the robot of venous blood collection robot and application | |
| CN107582073A (en) | A kind of self-service finger tip blood-taking device of clinical laboratory | |
| CN209172983U (en) | A kind of department of general surgery's medical instrument cleaning sterilizing device | |
| CN105054946B (en) | A kind of blood sampling auxiliary robot | |
| CN107049508A (en) | A kind of medical operation knife support | |
| CN101755884A (en) | Process and device for sterile blood sampling of live pigs | |
| CN105769220A (en) | Quality control system for guaranteeing biological safety of animal plasma, serum or blood | |
| CN204864175U (en) | Case and bag with sterilization and heating peritoneal dialysis liquid function | |
| CN109846902A (en) | A kind of preparation method of allosome source rich platelet gelator | |
| CN201350187Y (en) | Portable checkbox | |
| CN204542147U (en) | A kind of blood-taking device | |
| CN207479069U (en) | Scope Sterility Assurance system | |
| CN108853571A (en) | A kind of collagen protein sponge and its preparation process | |
| CN211751234U (en) | A degassing unit for medical treatment detector utensils | |
| CN204274894U (en) | The special ease of Use platform of nurse-in-charge | |
| CN201375571Y (en) | Disposable anus nursing set | |
| CN209695936U (en) | Medicinal ozone therapeutic instrument | |
| CN208808523U (en) | Disposable throat swab collector | |
| CN206279202U (en) | A kind of clinical treatment equipment of mother stem cell | |
| CN209695392U (en) | A kind of packet for culture of drawing blood | |
| CN211534798U (en) | Multifunctional venous blood sampling tool box | |
| CN205084055U (en) | Comprehensive sterilizer is used to stethoscope | |
| CN210406555U (en) | Anoectochilus formosanus inoculation inspection table | |
| CN211494865U (en) | Novel tool placing box for child medical doctors | |
| CN207785536U (en) | A kind of clinical laboratory's chlorination equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100630 |