CN101755669A - Exploration and utilization of wheat wild kindred species gene resource - Google Patents
Exploration and utilization of wheat wild kindred species gene resource Download PDFInfo
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- CN101755669A CN101755669A CN200810238636A CN200810238636A CN101755669A CN 101755669 A CN101755669 A CN 101755669A CN 200810238636 A CN200810238636 A CN 200810238636A CN 200810238636 A CN200810238636 A CN 200810238636A CN 101755669 A CN101755669 A CN 101755669A
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Abstract
Wild kindred species of wheat have various types, variation and extremely rich hereditary bases, contain a plurality of favorable genes that a plurality of wheat gene libraries lack and are precious gene resources in the wheat heredity improvement. The hereditary bases of wheat can be greatly enriched by transferring the favorable genes of exogenous species by a method of combining chromosome engineering and a plurality of techniques. The chromosome engineering technique is widely applied to the heredity improvement research of wheat as a result of maturation and effectiveness. According to the predesign of people, the chromosome engineering basic materials are utilized to change the chromosome composition by the chromosome operation of addition, replacement, reduction, translocation and the like so as to directionally change the technique system of heredity properties.
Description
Technical field
The excavation and the utilization of the nearly edge species gene resource of wheat wild belong to the Crop Genetics and Breeding field.
Background technology
The wild relatives of wheat is of a great variety, variation is various, hereditary basis is very abundant, is containing the excellent genes that is lacked in many wheat cdnas storehouse, is the valuable genetic resources of genetic improvement of wheat.The method that combines by chromosome engineering and multiple technologies shifts into wheat with the excellent genes of external source species, can greatly enrich the hereditary basis of wheat.At present, there be more than 20 with the wheat relative genus of wheat hybridizing success by distant hybridization, belong to (Roegneria), haynaldia villosa genus (Dasypyrum), Hordeum (Hordeum), wild barley genus (Critesion) etc. as Aegilops (Aegilops), Secale (Secale), couchgrass genus (Elytrigia), Agropyron (Agropyron), goose hat grass, wherein belong to the hybridization of wheat the most successful with couchgrass.Utilize the chromosome engineering technology chromosome set, chromosome and the chromosome segment of multiple nearly edge species such as rye, haynaldia villosa, middle couchgrass, lyme grass, tall and big goatweed, barley to be imported wheat, select a collection of amphidiploid, alien addition line, alien substitution and different translocation line, and bred the kind that large tracts of land is promoted on producing.
Summary of the invention
The chromosome engineering technology is because of its technology maturation and the fruitful genetic improvement research that is widely used in wheat.Chromosome engineering is meant the design in advance according to people, utilizes the chromosome engineering basic material, form by its chromosome of chromosome operation changes such as additional, replacement, reduction and transposition, and then the technical system of its hereditary capacity of directed change.Utilize the chromosome engineering technology can on chromosome base group, chromosome and three levels of chromosome segment, carry out to the wheat foreign gene transfer.
1, utilize chromosome set to the wheat foreign gene transfer
Foreign gene transfer mainly comprises synthetic complete amphidiploid and two kinds of approach of partial amphidiploid on chromosome base group level.Amphidiploid is meant the new species that has from a complete set of chromosome set be combined into of two parents, source and different in kinds fully, and its chromosome number is parents' chromosome number sum (Li Zhensheng, 1996).Amphidiploid fully between having cultivated both at home and abroad at present between multiple genus or having planted is as hexaploid triticale, OCTOPLOID TRITICALE, hexaploid tuftlet wheat, hexaploid lamb wheat and (Li Zhensheng, 1996 such as octoploid lamb wheat, octoploid little barley; Rong Junkang etc., 1989).Partial amphidiploid has only a part of chromosome base group of parents, and what wherein have important value is a complete set of chromosome and the additional type that indivedual chromosome base groups of affinity species are arranged that has wheat.This partial amphidiploid generally is by wheat and the hybridization of relationship kind, backcrosses with wheat parent and hybrid then, selects and the cytological Identification acquisition through separating again.China has bred multiple partial amphidiploid (Li Zhensheng, 1996 of affinity species such as common wheat and long fringe couchgrass, middle couchgrass, shore wheat; Fu Jie etc., 1993; Wang Hong is firm etc., and 2006).These valuable intermediate materials can be used as the bridge parent of further foreign gene transfer, have crucial value in the genetic improvement of wheat.
2, utilize wheat alien substitution foreign gene transfer
The wheat alien substitution is meant exogenous chromosome is shifted into wheat genetic background, replaces the formed distinct species material of chromosome of wheat.Can be divided between kind replacement two big classes between replacement and kind according to chromosome source.Wherein the disome alien substitution has utilization and researching value most between kind, it is not only the bridge material that shifts the external source excellent genes, also can be used as the basic material of homeologous relation and chromosome set evolutionary relationship etc. between hereditary effect, chromosome nonhomologous and the chromosome of wheat of research chromosome nonhomologous in Wheat Background.
3, utilize wheat alien addition line foreign gene transfer
Alien addition line is that the method for utilizing chromosome engineering is advanced the wheat genetic background to the chromosome transfer of carrying foreign gene and a kind of aneuploid material of forming, according to the number of additional exogenous chromosome, it can be divided into monomer, binary and multiple alien addition line, wherein the research of binary alien addition line and value are the highest, it is not only the bridge material of further creation alien substitution and different translocation line, also is affiliation, interaction of genes, gene expression and the important genetic stocks that carries out gene location between the research Origin of Species and evolution, the chromosome set.
4, utilize the alien translocation line foreign gene transfer
When the chromosome of the nearly edge species of wheat being imported the genetic background of wheat by chromosome addition and replacement, except that bringing some excellent genes into, many bad genes have also been introduced simultaneously, and the alien addition line and the alien substitution that have chromosome nonhomologous have lability in heredity, thereby have limited their utilization.The chromosome nonhomologous fragment that will have an excellent genes is transferred to and bred different translocation line on the chromosome of wheat is to address the above problem and utilize good heterologous gene to carry out the effective ways of wheat breed improvement.
Embodiment
1, utilize chromosome set to the wheat foreign gene transfer
Foreign gene transfer mainly comprises synthetic complete amphidiploid and two kinds of approach of partial amphidiploid on chromosome base group level.Amphidiploid is meant the new species that has from a complete set of chromosome set be combined into of two parents, source and different in kinds fully, and its chromosome number is parents' chromosome number sum (Li Zhensheng, 1996).Amphidiploid fully between having cultivated both at home and abroad at present between multiple genus or having planted is as hexaploid triticale, OCTOPLOID TRITICALE, hexaploid tuftlet wheat, hexaploid lamb wheat and (Li Zhensheng, 1996 such as octoploid lamb wheat, octoploid little barley; Rong Junkang etc., 1989).Partial amphidiploid has only a part of chromosome base group of parents, and what wherein have important value is a complete set of chromosome and the additional type that indivedual chromosome base groups of affinity species are arranged that has wheat.This partial amphidiploid generally is by wheat and the hybridization of relationship kind, backcrosses with wheat parent and hybrid then, selects and the cytological Identification acquisition through separating again.China has bred multiple partial amphidiploid (Li Zhensheng, 1996 of affinity species such as common wheat and long fringe couchgrass, middle couchgrass, shore wheat; Fu Jie etc., 1993; Wang Hong is firm etc., and 2006).These valuable intermediate materials can be used as the bridge parent of further foreign gene transfer, have crucial value in the genetic improvement of wheat.
2, utilize wheat alien substitution foreign gene transfer
The wheat alien substitution is meant exogenous chromosome is shifted into wheat genetic background, replaces the formed distinct species material of chromosome of wheat.Can be divided between kind replacement two big classes between replacement and kind according to chromosome source.Wherein the disome alien substitution has utilization and researching value most between kind, it is not only the bridge material that shifts the external source excellent genes, also can be used as the basic material of homeologous relation and chromosome set evolutionary relationship etc. between hereditary effect, chromosome nonhomologous and the chromosome of wheat of research chromosome nonhomologous in Wheat Background.
3, utilize wheat alien addition line foreign gene transfer
Alien addition line is that the method for utilizing chromosome engineering is advanced the wheat genetic background to the chromosome transfer of carrying foreign gene and a kind of aneuploid material of forming, according to the number of additional exogenous chromosome, it can be divided into monomer, binary and multiple alien addition line, wherein the research of binary alien addition line and value are the highest, it is not only the bridge material of further creation alien substitution and different translocation line, also is affiliation, interaction of genes, gene expression and the important genetic stocks that carries out gene location between the research Origin of Species and evolution, the chromosome set.
4, utilize the alien translocation line foreign gene transfer
When the chromosome of the nearly edge species of wheat being imported the genetic background of wheat by chromosome addition and replacement, except that bringing some excellent genes into, many bad genes have also been introduced simultaneously, and the alien addition line and the alien substitution that have chromosome nonhomologous have lability in heredity, thereby have limited their utilization.The chromosome nonhomologous fragment that will have an excellent genes is transferred to and bred different translocation line on the chromosome of wheat is to address the above problem and utilize good heterologous gene to carry out the effective ways of wheat breed improvement.
Claims (4)
1. utilize chromosome set to the wheat foreign gene transfer
Foreign gene transfer mainly comprises synthetic complete amphidiploid and two kinds of approach of partial amphidiploid on chromosome base group level, amphidiploid is meant to have from two parents fully, the new species of a complete set of chromosome set be combined into of source and different in kind, its chromosome number is parents' chromosome number sum, partial amphidiploid has only a part of chromosome base group of parents, what wherein have important value is a complete set of chromosome and the additional type that indivedual chromosome base groups of affinity species are arranged that has wheat, this partial amphidiploid generally is by wheat and the hybridization of relationship kind, backcross with wheat parent and hybrid then, select and the cytological Identification acquisition through separating again.
2. utilize wheat alien substitution foreign gene transfer
The wheat alien substitution is meant exogenous chromosome is shifted into wheat genetic background, replace the formed distinct species material of chromosome of wheat, can be divided between kind replacement two big classes between replacement and kind according to chromosome source, wherein the disome alien substitution has utilization and researching value most between kind, it is not only the bridge material that shifts the external source excellent genes, also can be used as the basic material of homeologous relation and chromosome set evolutionary relationship etc. between hereditary effect, chromosome nonhomologous and the chromosome of wheat of research chromosome nonhomologous in Wheat Background.
3. utilize wheat alien addition line foreign gene transfer
Alien addition line is that the method for utilizing chromosome engineering is advanced the wheat genetic background to the chromosome transfer of carrying foreign gene and a kind of aneuploid material of forming, according to the number of additional exogenous chromosome, it can be divided into monomer, binary and multiple alien addition line, wherein the research of binary alien addition line and value are the highest, it is not only the bridge material of further creation alien substitution and different translocation line, also is affiliation, interaction of genes, gene expression and the important genetic stocks that carries out gene location between the research Origin of Species and evolution, the chromosome set.
4. utilize the alien translocation line foreign gene transfer
By chromosome addition and replacement the chromosome of the nearly edge species of wheat is imported the genetic background of wheat, the chromosome nonhomologous fragment that will have an excellent genes is transferred to and bred different translocation line on the chromosome of wheat is to address the above problem and utilize good heterologous gene to carry out the effective ways of wheat breed improvement.
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| CN200810238636A CN101755669A (en) | 2008-12-19 | 2008-12-19 | Exploration and utilization of wheat wild kindred species gene resource |
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| CN200810238636A CN101755669A (en) | 2008-12-19 | 2008-12-19 | Exploration and utilization of wheat wild kindred species gene resource |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125447A (en) * | 2011-11-24 | 2013-06-05 | 上海西普尔-必凯实验动物有限公司 | Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1C3H/HeJ |
| CN103688846A (en) * | 2013-12-02 | 2014-04-02 | 西北农林科技大学 | Wheat-tritileymus 3D (3Ns) alien substitution line cultivation method and identification method |
| CN103719021A (en) * | 2012-10-10 | 2014-04-16 | 上海西普尔-必凯实验动物有限公司 | Construction for Chromosome 1 to replace wild mus musculus strain C57BL/6. Zaozhuang 2-Chr1 |
| CN110055317A (en) * | 2019-04-29 | 2019-07-26 | 中国农业科学院作物科学研究所 | The method of external source function candidate gene is excavated in wheat distance edge hybrid offspring's Small piece transposition system |
-
2008
- 2008-12-19 CN CN200810238636A patent/CN101755669A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125447A (en) * | 2011-11-24 | 2013-06-05 | 上海西普尔-必凯实验动物有限公司 | Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1C3H/HeJ |
| CN103125447B (en) * | 2011-11-24 | 2014-10-15 | 上海西普尔-必凯实验动物有限公司 | Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1C3H/HeJ |
| CN103719021A (en) * | 2012-10-10 | 2014-04-16 | 上海西普尔-必凯实验动物有限公司 | Construction for Chromosome 1 to replace wild mus musculus strain C57BL/6. Zaozhuang 2-Chr1 |
| CN103719021B (en) * | 2012-10-10 | 2017-06-20 | 上海西普尔-必凯实验动物有限公司 | No. 1 chromosome replaces the structure of the Chr1 of wild house mice strain C57BL/6. Zaozhuang 2 |
| CN103688846A (en) * | 2013-12-02 | 2014-04-02 | 西北农林科技大学 | Wheat-tritileymus 3D (3Ns) alien substitution line cultivation method and identification method |
| CN110055317A (en) * | 2019-04-29 | 2019-07-26 | 中国农业科学院作物科学研究所 | The method of external source function candidate gene is excavated in wheat distance edge hybrid offspring's Small piece transposition system |
| CN110055317B (en) * | 2019-04-29 | 2022-04-26 | 中国农业科学院作物科学研究所 | Method for discovering exogenous functional candidate genes in wheat distant hybridization progeny small-fragment translocation line |
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Open date: 20100630 |