CN101750397A - Method for accurately measuring bamboo wood starch content and rapid look-up table - Google Patents
Method for accurately measuring bamboo wood starch content and rapid look-up table Download PDFInfo
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Abstract
The invention discloses a method for accurately measuring bamboo wood starch content; I2 and KI are used for preparing detection reagent; one bamboo tube is cut off on the bamboo wood by taking any bamboo joint as a symmetric point, the bamboo tube is crushed into bamboo powder which is dried; the standard light absorption coefficient of the pure starch is determined to be 0.0136; the starch in the bamboo powder is completely extracted, after the filter residue is separated, the light absorption degree is detected, the measurement values of the light absorption degree is recorded more than 10 times, the arithmetic mean value of the measurement values is taken, and the arithmetic mean value and the standard light absorption coefficient are substituted to a computational formula, after calculation, accurate starch content value in the bamboo wood is obtained; on the basis of the measurement method, the invention further discloses a bamboo wood starch content rapid look-up table; the detection reagent is coated on the cross section of the bamboo wood, the color data after reaction is carried out is recorded, the data corresponds to the starch content value of the bamboo wood, measured by the method, so as to obtain the rapid look-up table; the technology can improve the accuracy of the starch content of the bamboo wood, reduce the measurement cost, is beneficial to leading bamboo wood enterprises to know the starch content of the bamboo wood fully before the bamboo wood is used, simplifies the measuring procedures of the starch content of the bamboo wood and is good for being popularized and applied in vast rural areas with simple and crude experiment conditions.
Description
Technical field
The invention belongs to the bamboo processing utilization field, be specifically related to a kind ofly before and after the bamboo processing utilization, content of starch in the bamboo wood is carried out accurate method for measuring.Simultaneously, the invention still further relates to a kind of quick parallel tables of bamboo wood starch content that utilizes this assay method to make up.
Background technology
Bamboo grove has the laudatory title of the world " second largest forest ", is the bamboo industry of primary raw material with the bamboo, is the pollution-free industry that generally acknowledge in the whole world.In recent years, along with the develop rapidly of Chinese national economy and improving constantly of living standards of the people, driven whole bamboo wood industry and developed rapidly.Because the demand of global timber resources enlarges day by day, and timber resources supply day is becoming tight, for satisfying the needs of the national economic development and people's lives, usable range, utilization ratio and the serviceable life of improving bamboo wood and bamboo wood product are imperative.And the bamboo wood product is as bamboo furniture (bamboo chair, bamboo bed, bamboo table etc.) and bamboo handicraft, bamboo wood sheet material (bamboo shaving plate, bamboo plywood, bamboo-wood plyboard etc.), because bamboo wood itself contains a large amount of starch materials, be easy to suffer the wood powder moth as the moth erosion of Lyctus brunneus Stephens, black wool bark beetle.Therefore, how to measure the content of starch in the bamboo wood quickly and accurately, adopt different disposal routes with bamboo wood, thereby reach the usable range and the life-span of improving the bamboo wood product, and determine that best cutting cycle just becomes the technical matters that needs to be resolved hurrily in the prior art at different content of starch.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, the method for content of starch in a kind of accurately measuring bamboo wood is provided, to satisfy related industry pressing for this technology.
The present invention also aims to provide a kind of quick parallel tables of bamboo wood starch content that makes up based on described assay method, use this chart can allow the user more convenient, determine content of starch in the bamboo wood apace.
Purpose of the present invention is achieved by the following technical programs.
Except as otherwise noted, percentage of the present invention is percent by weight.
The method of content of starch in a kind of accurately measuring bamboo wood may further comprise the steps:
1, detectable preparation: take by weighing 5.0g I
2With 7.5g KI, adding 100ml concentration is 35%～75% ethanolic solution, treats I
2Dissolve the back dilution fully with KI and be settled to 1L;
2, sample collection: on bamboo wood, be thick bamboo tube of symmetric points interceptings with any ring;
3, sample is produced: thick bamboo tube was crushed to the bamboo powder of 40～60 mesh sieves, the bamboo powder is dried by the fire to over dry again, and sealed standby;
4, measure the standard absorptivity of pure starch:
1. dispose pure starch solution: take by weighing the starch 0.1g of purity more than 99.9%, add the absorbent cotton of 100ml distilled water and 1g, be heated to boiling 10min, carry out suction filtration then while hot, and use hot wash, so that starch is fully by filtering;
2. after treating that filtrate is cooled to room temperature, be settled to 500ml, according to the preparation of the proportioning in the table 1 standard detection liquid, the standard detection liquid that detects different starch concentrations respectively is the absorbance numerical value at 580nm place in wavelength set then:
Table 1 typical curve solution ratio table
3. use statistical method, the linear dependence that calculates absorbance and starch solution concentration is absorptivity, as calculated, obtains the absorptivity of standard detection liquid at 580nm wavelength place and is: 0.0136;
5, the mensuration of content of starch in the bamboo powder:
1. extract the starch in the bamboo wood: take by weighing the standby bamboo powder of 1.0g, add 100ml distilled water, be heated to boiling 10min, carry out the suction filtration first time while hot; It is standby that concentrated hydrochloric acid and water are configured to aqueous hydrochloric acid solution by 1: 1 volume ratio; After for the first time suction filtration is finished, in the suction filtration residue, add the aqueous hydrochloric acid solution extracting 3 minutes of 25ml, carry out the suction filtration second time subsequently; After for the second time suction filtration was finished, suction filtration was for the third time carried out in the aqueous hydrochloric acid solution extracting 3 minutes of adding 25ml in the residue once more afterwards; Then in the suction filtration residue, add the concentrated hydrochloric acid of 25ml, shake gently, carry out suction filtration behind the 20s once more and separate; Last reusable heat water cleans residue, and adds an iodine liquid and detect the whether also residual starch that has, and if any residual starch, then continues to use hot water injection, suction filtration, collection filtrate;
2. the mensuration of absorbance: with the filter residue in the hydro-extractor separating filtrate, treat that filtrate is cooled to room temperature after, be settled to 500ml with distilled water, fully shake up; After leaving standstill 3～24 hours, use the supernatant of pipette, extract 25ml and the iodine liquid of 2.5ml respectively, and be settled to 50ml with distilled water, fully shaking up the back is that its absorbance is detected at the 580nm place at wavelength; And concentrated hydrochloric acid and water is configured to aqueous hydrochloric acid solution by 1: 19 volume ratio, get 2.5ml iodine liquid and be settled to absorbance reference as a setting behind the 50ml with this aqueous hydrochloric acid solution;
3. bamboo wood starch content calculates: the absorbance measuring value of record more than 10 times, get its arithmetic mean, and the formula below the substitution calculates the content of starch value W in the bamboo wood:
Wherein:
The percent of W content of starch;
The arithmetic mean value of α sample solution absorbance;
The absorptivity 0.0136 of the pure starch of A;
The bamboo opaque amount (unit: mg) that M is used to measure.
In the step 2, respectively intercept a thick bamboo tube by three positions, the high direction branch of bamboo wood bar upper, middle and lower, each thick bamboo tube all is symmetric points interceptings with the ring, and the length of each thick bamboo tube equals on this position bamboo wood the distance between two rings, and then measures the content of starch of bamboo wood different parts.
In order to allow the user can determine the content of starch of bamboo wood more easy when bamboo is felled, fast, on the basis of said determination method, the present invention also provides a kind of bamboo wood starch content quick parallel tables, and this chart adopts following method to make:
1, detectable preparation: take by weighing 5.0g I
2With 7.5g KI, adding 100ml concentration is 50% ethanolic solution, treats I
2Dissolve the back dilution fully with KI and be settled to 1L;
2, sample collection: schedule to last with a year, gathered respectively one of 1～7 year livings bamboo wood in every month respectively, divide base portion, middle part, three positions of taper to intercept a thick bamboo tube, and carry out mark with the position of bamboo wood of living in chronological order by the high direction of bar to every bamboo wood;
3, the color data of record sample: detectable is coated in the arbitrary cross section at each thick bamboo tube, reacts after 12～14 hours, measures the color data in thick bamboo tube cross section, and writes down the color data of each thick bamboo tube respectively;
4, the thick bamboo tube that will write down color data was crushed to the bamboo powder of 40～60 mesh sieves, dried by the fire to over dry, sealed standby;
5, adopt the method for content of starch in the aforementioned accurately measuring bamboo wood to measure the content of starch value of each sample bamboo powder;
6, measured content of starch numerical value is corresponding one by one with the color data of this sample, set up bamboo wood starch content rapid look-up table.
In the step 2, each thick bamboo tube all should be symmetric points with the ring, and length equals on this position bamboo wood the distance between two rings.
Preferably, base portion is chosen in liftoff 1 meter and intercepts the long thick bamboo tube of one section 2 slub space.It is long that the middle part is chosen in one section 2 slub space of center intercepting of whole section bamboo wood, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the thick bamboo tube of 1cm.One section 2 slub space of taper intercepting are long, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the thick bamboo tube of 1cm.
In the step 3, further, finish the image in the thick bamboo tube cross section of color reaction with scanner or camera record, and these images are corresponding one by one with the color data and the content of starch numerical value of this thick bamboo tube sample, be used to set up the quick parallel tables of bamboo wood starch content.
With respect to prior art, the present invention has the following advantages:
1, reduces the cost that bamboo wood starch content is measured;
2, improve the degree of accuracy that bamboo wood starch content is measured;
3, be beneficial to the serviceable life of improving the bamboo wood product, widen the usable range of bamboo wood product;
4, be beneficial to bamboo wood enterprise before using bamboo wood, bamboo wood starch content is had sufficient understanding;
5, simplify the program that bamboo wood starch content is measured, be beneficial in the simple and crude vast rural area of experiment condition and promote.
Embodiment
The present invention is described in further detail below by embodiment, but they are not limitation of the invention.
Embodiment 1
Measure the content of starch of imperial bamboo different parts:
1, takes by weighing 5g I earlier
2With 7.5g KI, adding 100ml concentration is that 50% ethanolic solution is with I
2With KI dissolving, after treating to dissolve fully again dilution be settled to 1L.
2, get one of 4 years imperial bamboo wood of life of Simao Diqu in October, 2008, divide base portion, middle part, three positions of taper respectively to gather a thick bamboo tube by the high direction of bar.
3, on three thick bamboo tubes of base portion, middle part, taper, split respectively and get the bamboo chip that a slice chord length is 5cm, respectively bamboo chip is placed drying in oven, split again after the taking-up and be small pieces, put into comminutor and pulverize, with 40-60 mesh sieve screening bamboo powder.Again the bamboo powder is put into baking oven and dry by the fire to over dry, stand-by with the encapsulation of plastic cement sealed bag.
4, configuration starch standard detection solution: accurately take by weighing purity at the starch 0.1g more than 99.9%, add the absorbent cotton of 100ml distilled water and 1g, be heated to boiling 10min.Carry out suction filtration then while hot, and, guarantee that starch is fully by filtering with 80 ℃ hot wash.After the filtrate that makes is cooled to room temperature, be settled to 500ml.After configuring standard detection solution according to the proportioning of each solution in the table 2, detect each solution absorbency numerical value at the 580nm place:
Table 2 typical curve solution ratio table
Use statistical method, calculation of starch solution concentration and absorbance correlativity are absorptivity, and obtaining starch is that the absorptivity at 580nm place is at wavelength: 0.0136.
5, take by weighing base portion, middle part, each 1.0g of taper bamboo wood powder respectively, add 100ml distilled water respectively, be heated to boiling 10min.While hot solution is carried out the suction filtration first time; After for the first time suction filtration was finished, the volume ratio of adding concentrated hydrochloric acid and water was 1: 1 hydrochloric acid solution 25ml in the residue, and the suction filtration second time is carried out in extracting 3 minutes subsequently; After for the second time suction filtration was finished, the volume ratio of adding concentrated hydrochloric acid and water was 1: 1 hydrochloric acid solution 25ml in the residue once more, and suction filtration is for the third time carried out in extracting 3 minutes; After suction filtration is finished for the third time, add the concentrated hydrochloric acid of 20ml in the residue, shake gently and make its mixing, carrying out suction filtration the 4th time behind the 20s separates.After the 4th time suction filtration is finished, clean residue with 80 ℃ hot water, suction filtration adds an iodine liquid at last to residue once more, detects the whether also residual starch that has.If any residual starch, continue hot water injection, suction filtration with 80 ℃.Filtrate is if contain filter residue, and available hydro-extractor separates.After treating that starch filtrate is cooled to room temperature, filtrate and cleansing solution together poured in the volumetric flask be settled to 500ml, fully shake up with distilled water.Use the starch supernatant of pipette, extract 25ml and the KI-I of 2.5ml respectively
2Liquid mixes, and uses the distilled water constant volume to 50ml then respectively, fully shakes up, and detects its absorbance under the wavelength of 580nm.Get 2.5mlKI-I simultaneously
2Liquid is settled to absorbance reference as a setting behind the 50ml with the hydrochloric acid solution of 1+19.
Each bamboo powder sample is respectively got 10 absorbance measuring values, and gets arithmetic mean.Formula below the substitution calculates the content of starch value at each position, specifically sees Table 3.
Wherein:
The percent of W content of starch;
The arithmetic mean value of α sample solution absorbance;
The absorptivity 0.0136 of the pure starch of A;
The bamboo opaque amount (unit: mg) that M is used to measure;
Each position content of starch of 4 years imperial bamboo woods of life of table 3 in October, 2008 Simao Diqu
| Specimen coding | Sample weight g | Absorptivity | Absorbance | Starch-containing amount g | The starch percentage composition |
| Base portion | ??1.00 | ??0.0136 | ??0.186 | ??0.0290 | ??2.91％ |
| The middle part | ??1.00 | ??0.0136 | ??0.168 | ??0.0224 | ??2.23％ |
| Specimen coding | Sample weight g | Absorptivity | Absorbance | Starch-containing amount g | The starch percentage composition |
| Taper | ??1.00 | ??0.0136 | ??0.163 | ??0.0205 | ??2.05％ |
Embodiment 2
Repeat embodiment 1, following difference is arranged: the hot water with 60 ℃ during suction filtration cleans residue.
Embodiment 3
Repeat embodiment 1, following difference is arranged: the hot water with 40 ℃ during suction filtration cleans residue.
Embodiment 4
Set up the imperial bamboo content of starch rapid look-up table of Yunnan Simao Diqu life in 1～5 year:
1, reagent preparation: take by weighing 5.0g I
2With 7.5g KI, adding 100ml concentration is 50% ethanolic solution, treats I
2Dissolve the back fully with KI and be settled to 1L with distilled water diluting;
2, sample collection: at the Yunnan Simao Diqu, scheduled to last with 1 year, gathered each one of 1～5 year living bamboo wood in every month respectively, divide base portion (2 slub lengths are upwards got at liftoff 1 meter), middle part (rounding one section 2 slub length in center of bamboo), three positions of taper (getting 2 rings that the diameter of a cross-section of a tree trunk 1.3 meters above the ground is not less than 6cm) to intercept a thick bamboo tube to every bamboo wood by the high direction of bar, each thick bamboo tube is symmetric points with the ring, length equals the distance between two rings in this position, and in chronological order and be in the bamboo wood different parts and carry out mark;
3, the color data of record sample: the xsect at each thick bamboo tube is coated detectable, reacts after 12～14 hours, measures the color and the record data of each thick bamboo tube respectively with tintmeter;
4, the thick bamboo tube that will write down color data was crushed to the bamboo powder of 40～60 mesh sieves, dried by the fire to over dry, sealed standby;
5, adopt the method for content of starch in the aforementioned accurately measuring bamboo wood to measure the content of starch of each sample bamboo powder, and with this content of starch numerical value as sample.
6, measured content of starch numerical value is corresponding one by one with the color data of this sample, obtain bamboo wood starch content rapid look-up table, see table 4 for details.
Application Example 1
Measured its content of starch in any imperial bamboo of Yunnan Simao Diqu collection in 2009:
1, reagent preparation: take by weighing 5.0g I
2With 7.5g KI, adding 100ml concentration is 50% ethanolic solution, treats I
2Dissolve the back fully with KI and be settled to 1L with distilled water diluting;
2, sample collection: adopt any three the imperial bamboos of Simao Diqu life in 2009 4 years as the identification sample, and sample is numbered.
3, the color data of record sample: every sample bamboo is according to base portion, middle part, taper, respectively get one with the ring be symmetric points, length equal two slub spaces in this position from thick bamboo tube, the xsect of every thick bamboo tube is polished, coat detectable, react after 12～14 hours, measure the color and the record data of each thick bamboo tube respectively with tintmeter; Data and table 4 contrast with record, can obtain the content of starch at each position, base portion color data L=59.22 as sample bamboo one, a=9.56, b=22.49 finds the L value of the imperial bamboo base portion of life in 4 years July, a value in table 4, b value and its are the most approaching, and then the content of starch that obtains the base portion of sample bamboo one is 0.02%.Find the content of starch such as the table 5 at three each positions of sample bamboo respectively with this method, and and then understand the content of starch basic condition of whole bamboo wood and even full wafer bamboo grove, and understand the growing state of bamboo grove.
The imperial bamboo content of starch results of comparison of table 5 Simao Diqu life in 4 years
Claims (6)
1. the method for content of starch in the accurately measuring bamboo wood may further comprise the steps:
(1) detectable preparation: take by weighing 5.0g I
2With 7.5g KI, adding 100ml concentration is 35%～75% ethanolic solution, treats I
2Dissolve the back dilution fully with KI and be settled to 1L;
(2) sample collection: on bamboo wood, be thick bamboo tube of symmetric points interceptings with any ring;
(3) sample is produced: thick bamboo tube was crushed to the bamboo powder of 40～60 mesh sieves, the bamboo powder is dried by the fire to over dry again, and sealed standby;
(4) the standard absorptivity of the pure starch of mensuration:
1. dispose pure starch solution: take by weighing the starch 0.1g of purity more than 99.9%, add the absorbent cotton of 100ml distilled water and 1g, be heated to boiling 10min, carry out suction filtration then while hot, and use hot wash, so that starch is fully by filtering;
2. after treating that filtrate is cooled to room temperature, be settled to 500ml, according to the preparation of the proportioning in the table 1 standard detection liquid, the standard detection liquid that detects different starch concentrations respectively is the absorbance numerical value at 580nm place in wavelength set then:
Table 1 typical curve solution ratio table
3. use statistical method, the linear dependence that calculates absorbance and starch solution concentration is absorptivity, as calculated, obtains the absorptivity of standard detection liquid at 580nm wavelength place and is: 0.0136;
(5) mensuration of content of starch in the bamboo powder:
1. extract the starch in the bamboo powder: take by weighing the standby bamboo powder of 1.0g, add 100ml distilled water, be heated to boiling 10min, carry out the suction filtration first time while hot; It is standby that concentrated hydrochloric acid and water are configured to aqueous hydrochloric acid solution by 1: 1 volume ratio; After for the first time suction filtration is finished, in the suction filtration residue, add the aqueous hydrochloric acid solution extracting 3 minutes of 25ml, carry out the suction filtration second time subsequently; After for the second time suction filtration was finished, suction filtration was for the third time carried out in the aqueous hydrochloric acid solution extracting 3 minutes of adding 25ml in the residue once more afterwards; Then in the suction filtration residue, add the concentrated hydrochloric acid of 25ml, shake gently, carry out suction filtration behind the 20s once more and separate; Last reusable heat water cleans residue, and adds an iodine liquid and detect the whether also residual starch that has, and if any residual starch, then continues to use hot water injection, suction filtration, collection filtrate;
2. the mensuration of absorbance: with the filter residue in the hydro-extractor separating filtrate, treat that filtrate is cooled to room temperature after, be settled to 500ml with distilled water, fully shake up; After leaving standstill 3～24 hours, use the supernatant of pipette, extract 25ml and the iodine liquid of 2.5ml respectively, and be settled to 50ml with distilled water, fully shaking up the back is that its absorbance is detected at the 580nm place at wavelength; And concentrated hydrochloric acid and water is configured to aqueous hydrochloric acid solution by 1: 19 volume ratio, get 2.5ml iodine liquid and be settled to absorbance reference as a setting behind the 50ml with this aqueous hydrochloric acid solution;
3. bamboo wood starch content calculates: write down 10 times or above absorbance measuring value, get its arithmetic mean, the formula below the substitution calculates the content of starch value W in the bamboo wood:
Wherein:
The percent of W content of starch;
The arithmetic mean value of α sample solution absorbance;
The absorptivity 0.0136 of the pure starch of A;
The bamboo opaque amount (unit: mg) that M is used to measure.
2. the method for content of starch in the accurately measuring bamboo wood according to claim 1, it is characterized in that: in the step (2), respectively intercept a thick bamboo tube by three positions, the high direction branch of bamboo wood bar upper, middle and lower, each thick bamboo tube all is the symmetric points interceptings with the ring, the length of each thick bamboo tube equals on this position bamboo wood the distance between two rings, and then measures the content of starch of bamboo wood different parts.
3. the method for content of starch in the accurately measuring bamboo wood according to claim 1 is characterized in that: on the basis of this assay method, make a kind of bamboo wood starch content rapid look-up table, method for making may further comprise the steps:
(1) detectable preparation: take by weighing 5.0g I
2With 7.5g KI, adding 100ml concentration is 50% ethanolic solution, treats I
2Dissolve the back dilution fully with KI and be settled to 1L;
(2) sample collection: schedule to last with a year, gathered respectively one of 1～7 year livings bamboo wood in every month respectively, divide base portion, middle part, three positions of taper to intercept a thick bamboo tube, and carry out mark with the position of bamboo wood of living in chronological order by the high direction of bar to every bamboo wood;
(3) color data of record sample: detectable is coated in the arbitrary cross section at each thick bamboo tube, reacts after 12～14 hours, measures the color data in thick bamboo tube cross section, and writes down the color data of each thick bamboo tube respectively;
(4) thick bamboo tube that will write down color data was crushed to the bamboo powder of 40～60 mesh sieves, dried by the fire to over dry, sealed standby;
(5) measure the content of starch value of each sample bamboo powder according to the method for content of starch in the described accurately measuring bamboo wood of claim 1;
(6) measured content of starch numerical value is corresponding one by one with the color data of this sample, promptly obtain bamboo wood starch content rapid look-up table.
4. the method for content of starch in the accurately measuring bamboo wood according to claim 3 is characterized in that: in the step (2), each thick bamboo tube all is symmetric points with the ring, and length equals on this position bamboo wood the distance between two rings.
5. the method for content of starch in the accurately measuring bamboo wood according to claim 3 is characterized in that: in the step (2), base portion is chosen in liftoff 1 meter and intercepts the long thick bamboo tube of one section 2 slub space; It is long that the middle part is chosen in one section 2 slub space of center intercepting of whole section bamboo wood, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the thick bamboo tube of 1cm; One section 2 slub space of taper intercepting are long, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than the thick bamboo tube of 1cm.
6. the method for content of starch in the accurately measuring bamboo wood according to claim 3, it is characterized in that: in the step (3), further, finish the image in the thick bamboo tube cross section of color reaction with scanner or camera record, and these images are combined with the bamboo wood starch content rapid look-up table that step (6) obtains, obtain the quick parallel tables of bamboo wood starch content.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103592250A (en) * | 2013-11-08 | 2014-02-19 | 浙江省农业科学院 | Method for quickly measuring apparent amylose content of rice with high flux |
| CN109855928A (en) * | 2018-12-04 | 2019-06-07 | 界首市万花巢生物科技有限公司 | A method of extracting starch and content of starch detection from potato |
-
2009
- 2009-12-29 CN CN 200910163256 patent/CN101750397A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103592250A (en) * | 2013-11-08 | 2014-02-19 | 浙江省农业科学院 | Method for quickly measuring apparent amylose content of rice with high flux |
| CN109855928A (en) * | 2018-12-04 | 2019-06-07 | 界首市万花巢生物科技有限公司 | A method of extracting starch and content of starch detection from potato |
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Open date: 20100623 |