CN101748184A - Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product - Google Patents
Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product Download PDFInfo
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Abstract
The invention relates to a biodegradation test method of a synthetic oil and vegetable oil-based lubricating oil product. The collected activated sludge is accessed to a synthetic oil and vegetable oil-based lubricating oil degradation culture medium after acclimatization and the tested microorganism bacteria solution biomass in the degradation culture medium and the lubricating oil organic matter residue in a liquid phase culture medium are served as an evaluation method and standard for evaluating the biodegradation rate of the synthetic oil and vegetable oil-based lubricating oil after seven days of continuous shaking culture under constant temperature. The biodegradation test method is a biodegradation evaluation method of the synthetic oil and vegetable oil-based lubricating oil, which has simple and convenient operation, good data reproducibility and stable repeatability and is applicable to biodegradable lubricating oil with different specifications and viscosity grades. The method is applied in the biodegradation tests of castor-based fully-synthetic high-performance lubricating oil products and uses the castor oil which has the degradation rate of about 96% in the literature reports as the reference oil, the tested degradation rate of the castor oil is 97%, and the degradation rate of the castor-based fully-synthetic high-performance lubricating oil reaches up to 85%.
Description
[technical field]
The present invention relates to the evaluation method of biological degradation lubricating oil, the biodegradation test method of particularly a kind of synthetic oil and plant oil based lube product.
[background technology]
At present, many in the world countries have all begun the development and the use of biological degradability lubricating oil, therefore simultaneously also just have corresponding biodegradability establishment of standard, the lubricating oil biodegradability standard of various countries must be decided according to the social present situation of various countries.The biodegradation method of measuring lube product mainly contains: the one, by the consumption of mensuration oxygen and the generation of carbonic acid gas, evaluate biological degradation rate; The 2nd, by measuring lubricating oil, evaluate its biological degradation rate through microorganism culturing residual quantity later.These two methods are to use maximum test method principles at present.According to these two kinds of methods, formulated OECD 301B and the CEC L-33-A-93 method more widely used respectively.OECD 301B method complex operation, will cause the personal errors influence in experimentation big, influence the Repeatability and Reproducibility of test-results, but experimental result is the biological degradation requirement of reaction test sample conscientiously, final product is a carbonic acid gas, environment is not had secondary pollution, and this just causes the harshness to the test sample requirement; CEC L-33-A-93 method is easy and simple to handle, adopts infrared spectroscopic determination lubricating oil through cultivating residual quantity later, and the reliability of this method, the Repeatability and Reproducibility additive method of comparing will be got well, thereby is generally accepted and use.This method is applicable to the biological degradation qualification test of hydraulic efficiency oil and low-viscosity oil usually, for the higher lubricating oil of other viscosity, because the influence in the water quality liquid phase, profit dispersing property in its culturing process descends, profit is difficult to separation etc. in the follow-up nutrient solution treating processes, all can cause the test-results instability, and error is bigger, sometimes can be up to 60%, or even fall flat.
[summary of the invention]
The object of the present invention is to provide the biodegradation test method of a kind of synthetic oil and plant oil based lube product, can overcome the defective of prior art.Specific integral part according to synthetic oil and plant oil based lubricant formula can be utilized this characteristic by microorganism, by gathering the active sludge that obtains, through being linked into after the domestication in synthetic oil and the plant oil based lubricating oil degraded substratum, through cultivating, microbial inoculum biomass in the mensuration degraded substratum and the lubricating oil organic substance residues amount in the liquid phase medium are as the assessment method and the standard of evaluation synthetic oil and plant oil based lubricating oil biological degradation rate.The present invention is easy and simple to handle, and data reproduction and repeatability are reliable and stable, with low cost, is applicable to the Biodegradable lubricant oil of all size and viscosity grade.Has wide application prospect.
The step that the biodegradation test method of synthetic oil provided by the invention and plant oil based lube product comprises:
1) domestication of purpose bacterial classification: gather active sludge from sewage work, after aeration processing in 24～48 hours, be dissolved in the sterilized water, stir, obtain bacteria suspension, draw bacteria suspension in liquid domestication substratum, place 26～28 ℃ of thermostat containers lucifuge shaking culture 3～5 days under 180～200r/min, treat that substratum becomes muddy this bacterium liquid of back absorption and is inoculated in repeatedly in the identical domestication substratum, each inoculum size is the 5mL/100mL substratum.Cultivate according to above-mentioned culture condition.Dominant bacteria after the domestication is Rhodopseudomonas (Pseudomonas), Clostridium (Clostridium), Mycobacterium good gas heterotroph bacteriums such as (Mycobacterium).
Domestication substratum: KH
2PO
43.4g; Na
2HPO
41.5g; (NH
4)
2SO
44.0g; MgSO
40.7g; Yeast powder 0.01g; Castor-based lubricating oil 2g; Tap water 1000mL; Adjusting pH is 7.2-7.6
2) determining of seed culture growth curve: will tame the more vigorous bacterial classification of back growth fraction, adopt visible spectrophotometer under the 660nm wavelength, to measure mixed bacteria liquid OD (absorbancy) value, draw the growth curve of biological degradation mixed bacteria liquid, the bacterium liquid of new generation of selecting logarithmic phase to enliven is inoculated in the time in the degraded substratum.
Seed culture medium: peptone 1.4 quality %, NaCl 0.6 quality %, glucose 0.3 quality %, extractum carnis 0.5 quality %, KH
2PO
40.5 quality %, K
2HPO
40.4 quality %; Culture temperature is 37 ℃, and the medium pH value is 7.5.
3) and Experiment of Biodegradation of Lubricant: select eugonic bacterium liquid to be lubricated oily biodegradation test according to the seed culture growth curve, from seed culture medium, draw mixed bacteria liquid, being linked into ready needs adds in the lubricating oil biological degradation substratum of bacterium liquid, place 37 ℃, rotating speed is in the constant temperature oscillator of 200r/min, and biodegrade was cultivated 7 days in the darkroom.
Biodegradation test comprises:
(neutral bottle) 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid;
(test bottle) 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid+2g synthetic oil and plant oil based lubricating oil;
(reference test bottle) 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid+2g reference oil;
(poisoning bottle) the 100mL mineral base nutritive medium+plant oil based lubricating oil of 2g synthetic oil;
(reference poisons bottle) 100mL mineral base nutritive medium+2g reference oil.
Reference oil is that degradation rate reaches the Viscotrol C (bibliographical information) about 96%.
Biological degradation substratum mineral nutrition liquid: KH
2PO
43.4g; Na
2HPO
41.5g; (NH
4)
2SO
44.0g; MgSO
40.7g; Yeast powder 0.01g; Tap water 1000mL; Adjusting pH is 7.2-7.6; Reference oil can be selected the higher vegetables oil of biological degradation rate reported in the document.
4) sample pretreatment and check and analysis: inserting the biological medium of mixed bacteria liquid, after cultivating through 7 days, is 1% aqueous hydrochloric acid and 20gNaCl dissolving with massfraction, adopts ultrasonic cell disruption instrument that nutrient solution is carried out fragmentation; Is to detect index with TOC, pretreated sample is carried out the mensuration of TC (total carbon) and IC (inorganic carbon) respectively, try to achieve the TOC value according to TOC (total organic carbon) calculation formula (TOC=TC-IC).According to the changing conditions of TOC value before and after cultivating, try to achieve the TOC degradation rate of testing sample and reference sample, the biodegradability of analytic sample.
Ultrasonic disruption condition (adopting mode intermittently): 200W, 2min, work 2s stops 1s.After this step finished, ultrasonic wave can make the material heating in the container, and the temperature of this moment will remain in 20～25 ℃ the scope;
5) biological degradation rate is measured: the microorganism species biomass velocity of variation with in the microscopic examination water quality lubricating oil biological degradation substratum can be used as the qualitative index that this lubricating oil of evaluation is utilized by microorganism.
Adopt the total organic carbon detection method, the organism velocity of variation in the detection water quality substratum is as its biodegradable quantitative target, and calculation formula is as follows:
P: poison irreducible oil content in the bottle, % (mean value)
T: irreducible oil content in the test bottle, % (mean value)
According to consumption and microorganism species increasing amount in culturing process the biodegradability of jointly evaluating lubricating oil of lubricating oil in liquid phase water quality.This method has following improvement technically: reduced the water quality organic matter biodegradation cultivate in to the requirement for restriction of lubricating oil viscosity; Improve in the substratum overcritical to mineral base nutritive substance, adopted usual nutrient matter both can satisfy the biological degradation culture experiment; Biological degradation rate for lubricating oil, annotate from the another one aspect, water quality lubricating oil substratum is through after the microorganism culturing, behind ultrasonic cell-break, the microorganism cells content is discharged, adopt the organic substance residues in the total organic carbon analytical method detection aqueous solutions then, the lubricating oil organism of loss then generates carbonic acid gas, is used as the biological degradation rate measurement result of synthetic oil and plant oil based lubricating oil with this.
The present invention is a kind of biology techniques that utilizes the microbiological deterioration lube product of occurring in nature.This method is a sole carbon source with synthetic oil and plant oil based lube product, be equipped with other essential nutritive ingredients of enough nitrogenous sources and microorganism growth, be prepared into the group water solution substratum, add certain bioactive nature microorganism bacterial classification, under constant temp and logical oxygen condition, after cultivation after a while, the lube product in the group water solution substratum is utilized by microbial consumption, thereby reaches the purpose of degraded.Wherein the content assaying method of lubricating oil in water quality adopts the higher total organic carbon detection method of tolerance range.This method has been applied in the biological degradation testing experiment of the complete synthesis high perofmrnace lubricating oils product of castor-oil plant base, and with the Viscotrol C of biological degradation rate in the bibliographical information about 96% as reference oil, the Viscotrol C biological degradation rate that records is 97%, and the biological degradation rate of the complete synthesis high perofmrnace lubricating oils of castor-oil plant base has reached 85%.
The present invention is a kind of easy and simple to handle, data reproduction and stable synthetic oil and the plant base lubricating oil biological degradation assessment method of repeatability, is applicable to the Biodegradable lubricant oil of all size and viscosity grade.Easy and simple to handle, with low cost, have wide application prospect.
[description of drawings]
The growth curve of Fig. 1 mixed bacteria liquid.
[embodiment]
One, substratum preparation
Domestication substratum: KH
2PO
43.4g; Na
2HPO
41.5g; (NH
4)
2SO
44.0g; MgSO
40.7g; Yeast powder 0.01g; Castor-based lubricating oil 2g; Tap water 1000mL; Adjusting pH is 7.2-7.6
Seed culture medium: peptone 1.4%, NaCl 0.6%, glucose 0.3%, extractum carnis 0.5%, KH
2PO
40.5%, K
2HPO
40.4%; Culture temperature is 37 ℃, and the medium pH value is 7.5.
Degraded substratum mineral nutrition liquid: KH
2PO
43.4g; Na
2HPO
41.5g; (NH
4)
2SO
44.0g; MgSO
40.7g; Yeast powder 0.01g; Tap water 1000mL; Adjusting pH is 7.2-7.6.
Two, soil collection and degradation bacteria domestication
1) requirement of collection soil: gather from the active sludge in the aeration tank, sampling depth 15～30cm gets final product; The utensil of gathering is through soil collector and plastic closures bag after the sterilization.
2) soil and processing: after soil is gathered, after aeration processing in 24 hours, can sieve the bacterium test.To take by weighing (interior glaze pearl is some) 5.0g to the 45mL sterilized water for every part from the pedotheque of active sludge factory different azimuth collection, 10min (120r/min) fully vibrates, in the 250mL triangular flask, draw the 5mL bacteria suspension then in 100mL liquid domestication substratum, place 26～28 ℃ of thermostat containers lucifuge shaking culture 3～5 days under 180～200r/min, treat that substratum becomes muddy this bacterium liquid of back absorption and is inoculated in repeatedly in all identical domestication substratum of composition, concentration, each inoculum size is the 5mL/100mL substratum.Still cultivate according to above-mentioned culture condition.Dominant bacteria after the domestication is Rhodopseudomonas (Pseudomonas), Clostridium (Clostridium), Mycobacterium good gas heterotroph bacteriums such as (Mycobacterium), and total viable bacteria concentration is 1.0 * 10
5～1.0 * 10
6Individual/mL.
Three, seed culture
1) determining of seed culture growth curve: will tame the more vigorous above-mentioned bacterium liquid of back growth fraction, adopt visible spectrophotometer under the 660nm wavelength, to measure mixed bacteria liquid OD (absorbancy) value, draw the growth curve of biological degradation mixed bacteria liquid, select its best inoculation time.
2) seed enlarged culturing: will under certain temperature condition, logical oxygen situation, cultivate the stable stage of its growth through the microorganism mixed bacteria liquid after the domestication, and be linked in the lubricating oil degraded substratum as kind of daughter bacteria liquid.
Four, biological degradation is cultivated
1) preparation of culturing bottle: (neutral bottle) 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid; (test bottle) 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid+2g synthetic oil and plant base lubricating oil; (reference test bottle) 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid+2g reference oil; (poisoning bottle) 100mL mineral base nutritive medium+2g synthetic oil plant base lubricating oil; (reference poisons bottle) 100mL mineral base nutritive medium+2g reference oil.(annotate: reference oil can be selected higher vegetables oil such as the Viscotrol C of biological degradation rate reported in the document, and its biological degradation rate is 96%).
2) training method: select eugonic bacterium liquid to be lubricated the biodegradation test of oil according to the seed culture growth curve, from seed culture medium, draw the 1mL mixed bacteria liquid, being linked into above-mentioned ready needs adds in the Erlenmeyer flask of bacterium liquid, place 37 ℃, rotating speed is in the constant temperature oscillator of 200r/min, and cultivated 7 days in the darkroom.
Table 1 biological degradation row is tested required flask quantity and distribution
| Incubation period (my god) | The test bottle of every kind of oil to be measured | Every kind of oil to be measured poison bottle | The test bottle of every kind of reference oil | Every kind of reference oil poison bottle | Neutral bottle |
| ??0 | ??3 | ??- | ??3 | ??- | ??2 |
| ??21 | ??3 | ??2 | ??3 | ??2 | ??- |
Annotate: " 0 day " flask should be prepared after on-test again, and it is extracted at once
Five, nutrient solution is handled and the total organic carbon detection method
1) breakdown of emulsion and breaking cell wall: after incubation period finishes, in each Erlenmeyer flask, add about 1mL aqueous hydrochloric acid and 20gNaCl, NaCl dissolves by shaking, after treating to dissolve fully, adopt ultrasonic cell disruption instrument with the microorganism wall fragmentation in the substratum, emit content thereby make, also can reach the purpose of further breakdown of emulsion on the other hand.Ultrasonic disruption condition (adopting mode intermittently): 200W, 2min, work 2s stops 1s.After this step finished, ultrasonic wave can make the material heating in the container, and the temperature of this moment will remain in 20～25 ℃ the scope, if overheated, can be placed in the cooling bath and carry out.After every bottle of solution ultrasonic disruption finishes, liquid wash residual on the horn is recovered in the Erlenmeyer flask test of the next sample of being allowed for access with distilled water.
2) total organic carbon detects: this experiment characterizes the variation of lubricating oil with the variation of TOC content, promptly is to detect index with TOC, to the employing of the organic content mutation analysis in testing sample TOC method.
Pretreated sample is carried out the mensuration of TC (total carbon) and IC (inorganic carbon) respectively, try to achieve the TOC value according to TOC (total organic carbon) calculation formula (TOC=TC-IC).According to the changing conditions of TOC value before and after cultivating, try to achieve the TOC degradation rate of testing sample and reference sample, the biodegradability of analytic sample.
Six, biological degradation rate measuring method
1) microorganism species biomass: the microorganism species biomass velocity of variation with in the microscopic examination water quality lubricating oil biological degradation substratum can be used as the qualitative index that its lubricating oil of evaluation is utilized by microorganism.
2) biological degradation rate: adopt the total organic carbon analytical procedure, the organism velocity of variation in the detection water quality substratum is as its biodegradable quantitative target.Calculation formula is as follows:
P: poison irreducible oil content in the bottle, % (mean value)
T: irreducible oil content in the test bottle, % (mean value).
Concrete Application Example
Biological degradation with castor-based lubricating oil is determined as example, and reference oil is selected document (Wang Kun, Fang Jianhua, the research [J] of lubricating oil biological degradation rapid assay methods, petroleum journal, 2004,20 (6)) degradation rate of report reaches 96% Viscotrol C in the report, according to the aforesaid operations method:
One, the mixed bacteria liquid that filters out carries out seed culture and measures growth curve, determines best seed stage
The growth curve of mixed bacteria liquid, its result as shown in Figure 1.The growth curve of Fig. 1 mixed bacteria liquid.
By last figure growth curve as can be seen, the lag phase of mixed bacteria liquid is shorter, begins to enter stationary phase at 11h.In addition, enter decline phase for up to 11h the stationary phase of mixed bacteria liquid after cultivating 23h.
Two, the total organic carbon detected value that finishes residual substance in the Erlenmeyer flask of back is cultivated in the castor-based lubricating oil biological degradation.
Three, the biological degradation rate result calculates:
The biological degradation rate of Viscotrol C:
Table 1 Viscotrol C total organic carbon data (unit: g/100ml)
Neutral bottle total organic carbon mean value: 0.182203
Revise 0 day culturing bottle total organic carbon value: 0.866157,0.821157,0.862007;
Revise back mean value X=0.849774;
Standard deviation s=0.0203
Variation factor Cv=2.389%＜5%;
Revised test bottle and poison bottle data results such as following table:
Table 2 is revised back 0 day culturing bottle, test bottle and is poisoned a bottle data results (unit: g/100ml)
According to require variation relation conefficient Cv%＜5% to method, the biological degradation rate of the Viscotrol C of mensuration is 97.14%.
2, the complete synthesis lubricating oil biological degradation rate of castor-oil plant base
Table 3 lubricating oil total organic carbon data (unit: g/100ml)
Neutral bottle total organic carbon mean value is: 0.182203;
Revise 0 day culturing bottle total organic carbon value: 0.620081,0.599222,0.584017;
Revise back mean value X=0.601107;
Standard deviation s=0.0148;
Variation factor Cv=2.459%＜5%;
Revised test bottle and poison bottle data results such as following table:
Table 4 is revised back 0 day culturing bottle, test bottle and is poisoned a bottle data results (unit: g/100ml)
According to require variation relation conefficient Cv%＜5% to method, the biological degradation rate of the lubricating oil of mensuration is 85.49%.
As can be seen, adopt this method to cultivate through 6 days from last table data, the biological degradation rate of Viscotrol C and the complete synthesis lubricating oil of castor-oil plant base has reached 97.14% and 85.49% respectively, and the variation relation conefficient of parallel test is within the confidence level scope.
The effect explanation
1, to the mixed bacteria liquid seed culture of magnifying after the domestication, be to strengthen the activity of bacterium liquid in the degraded culture medium;
2, by the improvement to the degraded culture medium, reduced the requirement to the culture medium nutriment;
3, under the simulating natural environment condition by to the degraded culture medium condition of culture optimization, improved the ability of microbial degradation plant base lubricating oil, and the method for the CEC that compares, shortened degradation time, the dull and stereotyped computational methods of the bacterium liquid of cultivating early stage have also been simplified, not only can realize the repeatability that operates also greatly improving the biodegradation effect;
4, adopt the method for ultrasonic cell-break, the microorganism content is discharged, the microbial degradation artificial oil of mensuration and the ability of plant base lubricating oil are effective degradation capability.
Claims (6)
1. the biodegradation test method of a synthetic oil and plant oil based lube product is characterized in that the step that it comprises:
1) collection is from the active sludge of sewage work, after aeration processing in 24-48 hour, be dissolved in the sterilized water, stir, obtain bacteria suspension, draw this bacteria suspension in liquid domestication substratum, place 26～28 ℃ of thermostat containers lucifuge shaking culture 3～5 days under 180～200r/min, treat that substratum becomes muddy this bacterium liquid of back absorption and is inoculated in repeatedly in all identical domestication substratum of composition, concentration, each inoculum size is the 5mL/100mL substratum.Still cultivate according to above-mentioned culture condition.Dominant bacteria after the domestication is Rhodopseudomonas (Pseudomonas), Clostridium (Clostridium), Mycobacterium good gas heterotroph bacteriums such as (Mycobacterium), and total viable bacteria concentration is 1.0 * 10
5～1.0 * 10
6Individual/mL.
2) determining of seed culture growth curve: will tame the more vigorous bacterial classification of back growth fraction and in seed culture medium, cultivate, culture temperature is 37 ℃, adopt visible spectrophotometer under the 660nm wavelength, to measure mixed bacteria liquid OD value, draw the growth curve of biological degradation mixed bacteria liquid, the bacterium liquid of new generation of selecting logarithmic phase to enliven is inoculated in the time in the degraded substratum;
3) select eugonic bacterium liquid to be lubricated the biodegradation test of oil according to the seed culture growth curve, from seed culture medium, draw mixed bacteria liquid, being linked into ready needs adds in the lubricating oil biological degradation substratum mineral nutrition liquid of bacterium liquid, place 37 ℃, rotating speed is in the constant temperature oscillator of 200r/min, and biodegrade was cultivated 7 days in the darkroom;
Biodegradation test comprises:
Neutral bottle: 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid;
Test bottle: 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid+2g synthetic oil and plant base lubricating oil;
Reference test bottle: 100mL mineral base nutritive medium+1mL kind daughter bacteria liquid+2g reference oil;
Poison bottle: 100mL mineral base nutritive medium+2g synthetic oil plant base lubricating oil;
Reference poisons bottle: 100mL mineral base nutritive medium+2g reference oil;
4) biological medium of access mixed bacteria liquid, after cultivating through 7 days, aqueous hydrochloric acid with 1% and 20gNaCl dissolving adopt ultrasonic cell disruption instrument with the microorganism wall fragmentation in the substratum; Pretreated sample is carried out the mensuration of TC (total carbon) and IC (inorganic carbon) respectively, try to achieve the TOC value according to TOC (total organic carbon) calculation formula (TOC=TC-IC).According to the changing conditions of TOC value before and after cultivating, try to achieve the TOC degradation rate of testing sample and reference sample, the biodegradability of analytic sample.
5), biological degradation rate is measured:
With the microorganism species biomass velocity of variation in the microscopic examination water quality lubricating oil biological degradation substratum, can be used as the qualitative index that its lubricating oil of evaluation is utilized by microorganism;
Adopt the total organic carbon analytical procedure, the organism velocity of variation in the detection water quality substratum is as its biodegradable quantitative target, and calculation formula is as follows:
P: poison irreducible oil content in the bottle, % (mean value)
T: irreducible oil content in the test bottle, % (mean value).
2. method according to claim 1 is characterized in that described domestication substratum is: KH
2PO
43.4g; Na
2HPO
41.5g; (NH
4)
2SO
44.0g; MgSO
40.7g; Yeast powder 0.01g; Castor-based lubricating oil 2g; Tap water 1000mL; PH is 7.2-7.6.
3. method according to claim 1 is characterized in that described seed culture medium is: peptone 1.4%, NaCl 0.6%, glucose 0.3%, extractum carnis 0.5%, KH
2PO
40.5%, K
2HPO
40.4%; The pH value is 7.5.
4. method according to claim 1 is characterized in that described reference oil is that degradation rate reaches 96% Viscotrol C.
5. method according to claim 1 is characterized in that described biological degradation substratum mineral nutrition liquid is KH
2PO
43.4g; Na
2HPO
41.5g; (NH
4)
2SO
44.0g; MgSO
40.7g; Yeast powder 0.01g; It is 7.2-7.6 that tap water 1000mL regulates pH.
6. method according to claim 1 is characterized in that described ultrasonic disruption condition adopts mode intermittently: 200W, 2min, work 2s stops 1s, after this step finishes, ultrasonic wave can make the material heating in the container, and the temperature of this moment will remain in 20～25 ℃ the scope.
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| CN 200910245237 CN101748184A (en) | 2009-12-31 | 2009-12-31 | Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product |
| PCT/CN2010/079882 WO2011079725A1 (en) | 2009-12-31 | 2010-12-16 | Method for detecting biodegradation of synthetic oil and vegetable oil-based lubricating oil product |
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|---|---|---|---|
| CN 200910245237 CN101748184A (en) | 2009-12-31 | 2009-12-31 | Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product |
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| CN111103412B (en) * | 2018-10-25 | 2024-01-23 | 中国石油化工股份有限公司 | Rapid determination method for anaerobic feasibility of petrochemical wastewater |
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| CN101748184A (en) * | 2009-12-31 | 2010-06-23 | 天津南开大学蓖麻工程科技有限公司 | Biodegradation test method of synthetic oil and vegetable oil-based lubricating oil product |
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| CN103822895A (en) * | 2014-01-28 | 2014-05-28 | 天津南开大学蓖麻工程科技有限公司 | Indoor degradation analysis method for synthetic lubricating oil under non-single degradation factors |
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| CN106635863B (en) * | 2016-07-19 | 2018-09-11 | 桂林理工大学 | Anaerobic degradation handles the cultural method of the Clostridium strain YB-7 of oil extraction waste water |
| CN108196040A (en) * | 2018-01-12 | 2018-06-22 | 天津大学 | A kind of method for evaluating thiocarbamide urea formaldehyde biological degradability |
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